benzamidine sepharose 6b Search Results


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  • 98
    GE Healthcare benzamidine sepharose 6b
    SDS-PAGE analysis of purified protease. Concentrated culture supernatant was applied to a <t>benzamidine-Sepharose</t> 6B column (2 ml) in 50 mM Tris-HCl (pH 8.0)-0.5 M NaCl. The retained enzyme was eluted by applying 0.1 M glycine-HCl (pH 3.0). Fractions of 1 ml were collected and monitored for enzyme activity. Enzyme activity was determined by using azocoll as a substrate. Fractions showing proteolytic activity were pooled. Protein concentration was determined by the Bradford method, and this fraction was subjected to SDS-PAGE analysis under reducing conditions.
    Benzamidine Sepharose 6b, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 98/100, based on 178 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    GE Healthcare benzamidine sepharose 6b resin
    Affinity liquid chromatography of Crotalus durissus terrificus crude venom with <t>Benzamidine-Sepharose</t> 6B resin (column AxiChrom 100/300® (100 mm × 300 mm × 350 mL). The column was equilibrated with 0.05 M Tris-HCl pH 7.4 (buffer 1). Sample: 15 g of crude venom was suspended in buffer 1. The sample elution was carried out with 525 mL of buffer 1 followed by 525 mL of 0.05 M Tris-HCl/0.5 M NaCl pH 7.4 (buffer 2) and 1,050 mL of 0.02 M glycine pH 3.2 (buffer 3), at flow rate 10 mL/min, and collected 25 mL/tube. Absorbance 280 nm
    Benzamidine Sepharose 6b Resin, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 81/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/benzamidine sepharose 6b resin/product/GE Healthcare
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    90
    GE Healthcare benzamidine sepharose 6b column
    Affinity liquid chromatography of Crotalus durissus terrificus crude venom with <t>Benzamidine-Sepharose</t> 6B resin (column AxiChrom 100/300® (100 mm × 300 mm × 350 mL). The column was equilibrated with 0.05 M Tris-HCl pH 7.4 (buffer 1). Sample: 15 g of crude venom was suspended in buffer 1. The sample elution was carried out with 525 mL of buffer 1 followed by 525 mL of 0.05 M Tris-HCl/0.5 M NaCl pH 7.4 (buffer 2) and 1,050 mL of 0.02 M glycine pH 3.2 (buffer 3), at flow rate 10 mL/min, and collected 25 mL/tube. Absorbance 280 nm
    Benzamidine Sepharose 6b Column, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 38 article reviews
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    83
    GE Healthcare benzamidine sepharose 6b beads
    Affinity liquid chromatography of Crotalus durissus terrificus crude venom with <t>Benzamidine-Sepharose</t> 6B resin (column AxiChrom 100/300® (100 mm × 300 mm × 350 mL). The column was equilibrated with 0.05 M Tris-HCl pH 7.4 (buffer 1). Sample: 15 g of crude venom was suspended in buffer 1. The sample elution was carried out with 525 mL of buffer 1 followed by 525 mL of 0.05 M Tris-HCl/0.5 M NaCl pH 7.4 (buffer 2) and 1,050 mL of 0.02 M glycine pH 3.2 (buffer 3), at flow rate 10 mL/min, and collected 25 mL/tube. Absorbance 280 nm
    Benzamidine Sepharose 6b Beads, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 83/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    GE Healthcare benzamidin sepharose 6b
    Affinity liquid chromatography of Crotalus durissus terrificus crude venom with <t>Benzamidine-Sepharose</t> 6B resin (column AxiChrom 100/300® (100 mm × 300 mm × 350 mL). The column was equilibrated with 0.05 M Tris-HCl pH 7.4 (buffer 1). Sample: 15 g of crude venom was suspended in buffer 1. The sample elution was carried out with 525 mL of buffer 1 followed by 525 mL of 0.05 M Tris-HCl/0.5 M NaCl pH 7.4 (buffer 2) and 1,050 mL of 0.02 M glycine pH 3.2 (buffer 3), at flow rate 10 mL/min, and collected 25 mL/tube. Absorbance 280 nm
    Benzamidin Sepharose 6b, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 79/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    GE Healthcare benzamidine
    Inhibition by HAI-1–58K of MAT molecules with and without purification by <t>benzamidine</t> chromatography. A , SDS-PAGE and silver staining showing the removal of fragment Tyr 81 –Lys 379 . MAT before treatment with immobilized benzamidine ( lane
    Benzamidine, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 94/100, based on 201 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    SDS-PAGE analysis of purified protease. Concentrated culture supernatant was applied to a benzamidine-Sepharose 6B column (2 ml) in 50 mM Tris-HCl (pH 8.0)-0.5 M NaCl. The retained enzyme was eluted by applying 0.1 M glycine-HCl (pH 3.0). Fractions of 1 ml were collected and monitored for enzyme activity. Enzyme activity was determined by using azocoll as a substrate. Fractions showing proteolytic activity were pooled. Protein concentration was determined by the Bradford method, and this fraction was subjected to SDS-PAGE analysis under reducing conditions.

    Journal: Journal of Bacteriology

    Article Title: Proteome and Transcriptome Analysis of the Virulence Genes Regulated by the VirR/VirS System in Clostridium perfringens

    doi: 10.1128/JB.184.10.2587-2594.2002

    Figure Lengend Snippet: SDS-PAGE analysis of purified protease. Concentrated culture supernatant was applied to a benzamidine-Sepharose 6B column (2 ml) in 50 mM Tris-HCl (pH 8.0)-0.5 M NaCl. The retained enzyme was eluted by applying 0.1 M glycine-HCl (pH 3.0). Fractions of 1 ml were collected and monitored for enzyme activity. Enzyme activity was determined by using azocoll as a substrate. Fractions showing proteolytic activity were pooled. Protein concentration was determined by the Bradford method, and this fraction was subjected to SDS-PAGE analysis under reducing conditions.

    Article Snippet: Concentrated supernatant solution was applied to a benzamidine-Sepharose 6B (Amersham Pharmacia Biotech United Kingdom, Ltd., Buckinghamshire, England) affinity column (bed volume, 2 ml) equilibrated with 50 mM Tris-HCl (pH 8.0) containing 0.5 M NaCl.

    Techniques: SDS Page, Purification, Activity Assay, Protein Concentration

    Affinity liquid chromatography of Crotalus durissus terrificus crude venom with Benzamidine-Sepharose 6B resin (column AxiChrom 100/300® (100 mm × 300 mm × 350 mL). The column was equilibrated with 0.05 M Tris-HCl pH 7.4 (buffer 1). Sample: 15 g of crude venom was suspended in buffer 1. The sample elution was carried out with 525 mL of buffer 1 followed by 525 mL of 0.05 M Tris-HCl/0.5 M NaCl pH 7.4 (buffer 2) and 1,050 mL of 0.02 M glycine pH 3.2 (buffer 3), at flow rate 10 mL/min, and collected 25 mL/tube. Absorbance 280 nm

    Journal: The Journal of Venomous Animals and Toxins Including Tropical Diseases

    Article Title: Heterologous fibrin sealant derived from snake venom: from bench to bedside – an overview

    doi: 10.1186/s40409-017-0109-8

    Figure Lengend Snippet: Affinity liquid chromatography of Crotalus durissus terrificus crude venom with Benzamidine-Sepharose 6B resin (column AxiChrom 100/300® (100 mm × 300 mm × 350 mL). The column was equilibrated with 0.05 M Tris-HCl pH 7.4 (buffer 1). Sample: 15 g of crude venom was suspended in buffer 1. The sample elution was carried out with 525 mL of buffer 1 followed by 525 mL of 0.05 M Tris-HCl/0.5 M NaCl pH 7.4 (buffer 2) and 1,050 mL of 0.02 M glycine pH 3.2 (buffer 3), at flow rate 10 mL/min, and collected 25 mL/tube. Absorbance 280 nm

    Article Snippet: This was applied in an AxiChrom 100/300® chromatographic column (100 mm x 300 mm x 350 mL) (GE HealthCare Life Science, Sweden) encased with affinity resin Benzamidine Sepharose 6B® (GE HealthCare Life Science, Sweden) previously equilibrated with 525 mL of 0.05 M Tris-HCl pH 7.4 buffer (buffer 1).

    Techniques: Liquid Chromatography, Flow Cytometry

    Inhibition by HAI-1–58K of MAT molecules with and without purification by benzamidine chromatography. A , SDS-PAGE and silver staining showing the removal of fragment Tyr 81 –Lys 379 . MAT before treatment with immobilized benzamidine ( lane

    Journal: The Journal of Biological Chemistry

    Article Title: Identification of the Matriptase Second CUB Domain as the Secondary Site for Interaction with Hepatocyte Growth Factor Activator Inhibitor Type-1 *

    doi: 10.1074/jbc.M110.115816

    Figure Lengend Snippet: Inhibition by HAI-1–58K of MAT molecules with and without purification by benzamidine chromatography. A , SDS-PAGE and silver staining showing the removal of fragment Tyr 81 –Lys 379 . MAT before treatment with immobilized benzamidine ( lane

    Article Snippet: A 0.5-ml sample of HEPES buffer containing r-EK-treated pro-MAT was incubated for 30 min with 50 μl of a slurry of immobilized benzamidine (benzamidine-Sepharose 6B; GE Healthcare) at 22 °C with rocking.

    Techniques: Inhibition, Purification, Chromatography, SDS Page, Silver Staining