Journal: Cell Death & Disease
Article Title: Inhibition of lymphatic proliferation by the selective VEGFR-3 inhibitor SAR131675 ameliorates diabetic nephropathy in db/db mice
Figure Lengend Snippet: SAR131675 attenuates apoptosis and oxidative stress in the kidneys in db/db mice. Glomerulosclerosis and tubulointerstitial fibrosis were determined at 20 weeks in db/m and db/db mice treated with or without SAR131675. a Representative images of terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) staining in the glomeruli and tubules and quantitative data. b Representative western blots for B cell leukemia/lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax), and β-actin in the kidneys and quantitative data. c Twenty-four hour urinary 8-hydroxy-deoxyguanosine (8-OH-dG) and isoprostane levels. * P
Article Snippet: Western blot analysis Protein extracts were prepared from cultured cells and homogenates of mouse renal cortex or medulla by using mammalian protein extraction reagent (Intron Biotechnology, Gyeonggi-Do, Korea) lysis buffer to which protease inhibitors (Roche, Indianapolis) was added, and Western blots were performed with specific antibodies for VEGF-C (Invitrogen, Carlsbad, CA, USA), VEGFR-1 (Epitomics, Burlingame, CA, USA), VEGFR-2 (Cell Signaling Technology, Danvers, MA, USA) and VEGFR3 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), LYVE-1 (Novus Biologicals, Littleton, CO, USA), podoplanin (ReliaTech GmbH, Wolfenbüttel, Germany), α-SMA (Abcam, Cambridge, UK), fibronectin (ProteinTech, Chicago, IL, USA), inducible nitric oxide synthase (iNOS) (BD Biosciences, San Diego, CA, USA), CD68 (Bio-Rad, Richmond, CA, USA), arginase I (Santa Cruz Biotechnology, Santa Cruz, CA, USA), arginase II (Santa Cruz Biotechnology, Santa Cruz, CA, USA), B cell leukemia/lymphoma 2 (Bcl-2), Bcl-2-associated X protein (Bax) (Santa Cruz Biotechnology, Santa Cruz, CA, USA), and β-actin (Sigma-Aldrich, St. Louis, MO, USA).
Techniques: Mouse Assay, End Labeling, TUNEL Assay, Staining, Western Blot