Journal: Frontiers in Pharmacology
Article Title: The Long Non-coding RNA MEG3/miR-let-7c-5p Axis Regulates Ethanol-Induced Hepatic Steatosis and Apoptosis by Targeting NLRC5
Figure Lengend Snippet: Reducing MEG3 expression inhibits EtOH-induced hepatic apoptosis. (A,B) Effect of MEG3 on expression of protein Bax/Bcl2, cleaved caspase 3 and cleaved caspase 9 in EtOH-induced AML-12 cells. The expression of proteins were determined by western blot. β-actin served as a loading control. (C) Flow cytometry analyses of apoptosis. The abscissa represents FITC-Annexin V staining and the ordinate represents PI staining. Data shown are the mean ± SD from three independent experiments. ∗ P
Article Snippet: The categories and concentrations of primary antibodies: anti-PPAR-α (Rabbit, 1:800, ab8934, Abcam, United Kingdom), SREBP-1c (Rabbit, 1:800, ab28481, Abcam, United Kingdom), anti-Bax (Rabbit, 1:800, ab32503, Abcam, United Kingdom), anti-Bcl2 (Rabbit, 1:800, ab196495, Abcam, United Kingdom), anti-caspase 9 (Rabbit, 1:800, ab184786, Abcam, United Kingdom), and anti-NLRC5 (Rabbit, 1:800, ab117624, Abcam, United Kingdom); anti-caspase 3 (Rabbit, 1:800, #8242, Cell Signaling Tech, Danvers, MA, United States), anti-β-actin (Mouse, 1:300, sc47778, Santa Cruz, CA, United States).
Techniques: Expressing, Western Blot, Flow Cytometry, Cytometry, Staining