bbvi Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    New England Biolabs bbvi
    Characterization of in situ restriction digestion of dsDNA colonies. ( A ) Autoradiograms obtained from surface-released products labeled at their 3′ ends with [α- 33 P]ddNTP. Digestions were performed for 1 h at various concentrations (indicated) of <t>BbvI.</t> The gel images show <t>DNA</t> cleavage as a function of enzyme concentration (left) and near-quantitative cleavage of the 347-bases substrate to the expected products of 300–304 and 47 bases (right). ( B ) Electropherograms obtained from surface-released products at different enzymatic reaction times for 0.25 µ/µl BbvI followed by 3′ end labeling with ddNTP-Cy5.
    Bbvi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 74 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bbvi/product/New England Biolabs
    Average 99 stars, based on 74 article reviews
    Price from $9.99 to $1999.99
    bbvi - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    99
    Thermo Fisher bsexi bbvi
    Characterization of in situ restriction digestion of dsDNA colonies. ( A ) Autoradiograms obtained from surface-released products labeled at their 3′ ends with [α- 33 P]ddNTP. Digestions were performed for 1 h at various concentrations (indicated) of <t>BbvI.</t> The gel images show <t>DNA</t> cleavage as a function of enzyme concentration (left) and near-quantitative cleavage of the 347-bases substrate to the expected products of 300–304 and 47 bases (right). ( B ) Electropherograms obtained from surface-released products at different enzymatic reaction times for 0.25 µ/µl BbvI followed by 3′ end labeling with ddNTP-Cy5.
    Bsexi Bbvi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bsexi bbvi/product/Thermo Fisher
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    bsexi bbvi - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    bbvi  (Roche)
    86
    Roche bbvi
    Segregation of the c.1922G > A MANBA mutation in the family . A, Family pedigree. The arrow denotes the proband. Genetic status for the c.1922G > A mutation is indicated by black and white symbols. B, Restriction enzyme analysis of exon 14 in the patient, his family and three control subjects (denoted C1 to C3). PCR-amplified exon 14 (a 423 bp fragment) was digested with <t>MaeIII</t> or <t>BbvI</t> and analyzed by gel electrophoresis.
    Bbvi, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bbvi/product/Roche
    Average 86 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    bbvi - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    92
    Thermo Fisher bbvi
    Scheme showing the strategy for hairpin-bisulfite PCR. ( A ) Genomic <t>DNA</t> sequence corresponding to the domain of the Peg3 imprinting control region analyzed in this study. CpG sites are numbered and shown in bold. The <t>BbvI</t> recognition/cleavage site is indicated in gray. Sequences indicated by solid arrows correspond to the modified primers (Cs substituted by Ts) used for PCR. ( B ) Diagram illustrating the ligation of the BbvI-cleavaged genomic DNA with the hairpin (labeled in gray). The resulting DNA is bisulfite-converted and subjected to PCR amplification with the oligonucleotides indicated in (A). The resulting amplification bands were cloned and Sanger sequenced (see Figure 5B and C ).
    Bbvi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bbvi/product/Thermo Fisher
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    bbvi - by Bioz Stars, 2020-04
    92/100 stars
      Buy from Supplier

    86
    Sangon Biotech bbvi
    Scheme showing the strategy for hairpin-bisulfite PCR. ( A ) Genomic <t>DNA</t> sequence corresponding to the domain of the Peg3 imprinting control region analyzed in this study. CpG sites are numbered and shown in bold. The <t>BbvI</t> recognition/cleavage site is indicated in gray. Sequences indicated by solid arrows correspond to the modified primers (Cs substituted by Ts) used for PCR. ( B ) Diagram illustrating the ligation of the BbvI-cleavaged genomic DNA with the hairpin (labeled in gray). The resulting DNA is bisulfite-converted and subjected to PCR amplification with the oligonucleotides indicated in (A). The resulting amplification bands were cloned and Sanger sequenced (see Figure 5B and C ).
    Bbvi, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bbvi/product/Sangon Biotech
    Average 86 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    bbvi - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    86
    Thermo Fisher restriction endonuclease bbvi
    Distribution of RFLP patterns of cbbL gene fragments from the red-like cbbL gene libraries of the HKO, HSM, and HNPK soil samples. cbbL <t>PCR</t> products were digested with the restriction endonuclease <t>BbvI.</t>
    Restriction Endonuclease Bbvi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction endonuclease bbvi/product/Thermo Fisher
    Average 86 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    restriction endonuclease bbvi - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    86
    Thermo Fisher fastdigest bbvi
    Distribution of RFLP patterns of cbbL gene fragments from the red-like cbbL gene libraries of the HKO, HSM, and HNPK soil samples. cbbL <t>PCR</t> products were digested with the restriction endonuclease <t>BbvI.</t>
    Fastdigest Bbvi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fastdigest bbvi/product/Thermo Fisher
    Average 86 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    fastdigest bbvi - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    91
    Thermo Fisher bbvi restriction enzyme
    Distribution of RFLP patterns of cbbL gene fragments from the red-like cbbL gene libraries of the HKO, HSM, and HNPK soil samples. cbbL <t>PCR</t> products were digested with the restriction endonuclease <t>BbvI.</t>
    Bbvi Restriction Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bbvi restriction enzyme/product/Thermo Fisher
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bbvi restriction enzyme - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    85
    Thermo Fisher bbvi restriction enzyme solution
    Distribution of RFLP patterns of cbbL gene fragments from the red-like cbbL gene libraries of the HKO, HSM, and HNPK soil samples. cbbL <t>PCR</t> products were digested with the restriction endonuclease <t>BbvI.</t>
    Bbvi Restriction Enzyme Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bbvi restriction enzyme solution/product/Thermo Fisher
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    bbvi restriction enzyme solution - by Bioz Stars, 2020-04
    85/100 stars
      Buy from Supplier

    Image Search Results


    Characterization of in situ restriction digestion of dsDNA colonies. ( A ) Autoradiograms obtained from surface-released products labeled at their 3′ ends with [α- 33 P]ddNTP. Digestions were performed for 1 h at various concentrations (indicated) of BbvI. The gel images show DNA cleavage as a function of enzyme concentration (left) and near-quantitative cleavage of the 347-bases substrate to the expected products of 300–304 and 47 bases (right). ( B ) Electropherograms obtained from surface-released products at different enzymatic reaction times for 0.25 µ/µl BbvI followed by 3′ end labeling with ddNTP-Cy5.

    Journal: Nucleic Acids Research

    Article Title: BTA, a novel reagent for DNA attachment on glass and efficient generation of solid-phase amplified DNA colonies

    doi: 10.1093/nar/gnj023

    Figure Lengend Snippet: Characterization of in situ restriction digestion of dsDNA colonies. ( A ) Autoradiograms obtained from surface-released products labeled at their 3′ ends with [α- 33 P]ddNTP. Digestions were performed for 1 h at various concentrations (indicated) of BbvI. The gel images show DNA cleavage as a function of enzyme concentration (left) and near-quantitative cleavage of the 347-bases substrate to the expected products of 300–304 and 47 bases (right). ( B ) Electropherograms obtained from surface-released products at different enzymatic reaction times for 0.25 µ/µl BbvI followed by 3′ end labeling with ddNTP-Cy5.

    Article Snippet: Taq polymerase was supplied from Promega, Terminal Transferase, BbvI and bacteriophage lambda DNA were from New England Biolabs.

    Techniques: In Situ, Labeling, Concentration Assay, End Labeling

    Characterization of DNA colonies generated using BTA chemistry by Sybr-Green I staining. Histograms of colonies average intensity ( A ) Before BbvI digestion colonies are double stranded and their length is 347 bp. ( B ) After BbvI digestion, colonies are still double stranded and their length is reduced to 43 bp, showing consequently a lower average intensity.

    Journal: Nucleic Acids Research

    Article Title: BTA, a novel reagent for DNA attachment on glass and efficient generation of solid-phase amplified DNA colonies

    doi: 10.1093/nar/gnj023

    Figure Lengend Snippet: Characterization of DNA colonies generated using BTA chemistry by Sybr-Green I staining. Histograms of colonies average intensity ( A ) Before BbvI digestion colonies are double stranded and their length is 347 bp. ( B ) After BbvI digestion, colonies are still double stranded and their length is reduced to 43 bp, showing consequently a lower average intensity.

    Article Snippet: Taq polymerase was supplied from Promega, Terminal Transferase, BbvI and bacteriophage lambda DNA were from New England Biolabs.

    Techniques: Generated, SYBR Green Assay, Staining

    RT-PCR analysis of GluR5 and GluR6 editing in single cerebellar granule cells A , GluR5 RT-PCR amplification products from 3 granule cells. The samples were separated on a 6% polyacrylamide gel that was stained with ethidium bromide and photographed. The lane marked (-) contains undigested RT-PCR products. Lanes 1–3 show RT-PCR products from each cell after digestion with Bbv I. The sizes of the uncut GluR5 product (313 bp) and the edited (R, 187 bp) and unedited (Q, 106 bp) restriction fragments are indicated at the sides of each panel. The lane labelled M contains a 1 kb DNA ladder (Life Sciences) as a size standard. B , sequence analysis of GluR6-specific RT-PCR products obtained from 2 granule cells (GC-5–4 and GC-5-3). The position of the Q/R site is indicated. Note that for both cells a termination signal is only detected in the ddG lane.

    Journal: The Journal of Physiology

    Article Title: High-affinity kainate-type ion channels in rat cerebellar granule cells

    doi: 10.1111/j.1469-7793.1998.401bk.x

    Figure Lengend Snippet: RT-PCR analysis of GluR5 and GluR6 editing in single cerebellar granule cells A , GluR5 RT-PCR amplification products from 3 granule cells. The samples were separated on a 6% polyacrylamide gel that was stained with ethidium bromide and photographed. The lane marked (-) contains undigested RT-PCR products. Lanes 1–3 show RT-PCR products from each cell after digestion with Bbv I. The sizes of the uncut GluR5 product (313 bp) and the edited (R, 187 bp) and unedited (Q, 106 bp) restriction fragments are indicated at the sides of each panel. The lane labelled M contains a 1 kb DNA ladder (Life Sciences) as a size standard. B , sequence analysis of GluR6-specific RT-PCR products obtained from 2 granule cells (GC-5–4 and GC-5-3). The position of the Q/R site is indicated. Note that for both cells a termination signal is only detected in the ddG lane.

    Article Snippet: The GluR5 and GluR6 RT-PCR products amplified from single granule cells (10 μl of a 50 μl reaction) were digested for 4–6 h at 37°C with 4–6 U of Bbv I (New England Biolabs, Beverly, MA, USA).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Amplification, Staining, Sequencing

    Segregation of the c.1922G > A MANBA mutation in the family . A, Family pedigree. The arrow denotes the proband. Genetic status for the c.1922G > A mutation is indicated by black and white symbols. B, Restriction enzyme analysis of exon 14 in the patient, his family and three control subjects (denoted C1 to C3). PCR-amplified exon 14 (a 423 bp fragment) was digested with MaeIII or BbvI and analyzed by gel electrophoresis.

    Journal: BMC Medical Genetics

    Article Title: A MANBA mutation resulting in residual beta-mannosidase activity associated with severe leukoencephalopathy: a possible pseudodeficiency variant

    doi: 10.1186/1471-2350-10-84

    Figure Lengend Snippet: Segregation of the c.1922G > A MANBA mutation in the family . A, Family pedigree. The arrow denotes the proband. Genetic status for the c.1922G > A mutation is indicated by black and white symbols. B, Restriction enzyme analysis of exon 14 in the patient, his family and three control subjects (denoted C1 to C3). PCR-amplified exon 14 (a 423 bp fragment) was digested with MaeIII or BbvI and analyzed by gel electrophoresis.

    Article Snippet: For restriction enzyme analysis of exon 14, amplicons were incubated with MaeIII or BbvI (Roche Diagnostics, Germany, and New England Biolabs, MA, respectively), and analysed on a 1.8% agarose gel.

    Techniques: Mutagenesis, Polymerase Chain Reaction, Amplification, Nucleic Acid Electrophoresis

    Scheme showing the strategy for hairpin-bisulfite PCR. ( A ) Genomic DNA sequence corresponding to the domain of the Peg3 imprinting control region analyzed in this study. CpG sites are numbered and shown in bold. The BbvI recognition/cleavage site is indicated in gray. Sequences indicated by solid arrows correspond to the modified primers (Cs substituted by Ts) used for PCR. ( B ) Diagram illustrating the ligation of the BbvI-cleavaged genomic DNA with the hairpin (labeled in gray). The resulting DNA is bisulfite-converted and subjected to PCR amplification with the oligonucleotides indicated in (A). The resulting amplification bands were cloned and Sanger sequenced (see Figure 5B and C ).

    Journal: Nucleic Acids Research

    Article Title: Strand-specific CpG hemimethylation, a novel epigenetic modification functional for genomic imprinting

    doi: 10.1093/nar/gkx518

    Figure Lengend Snippet: Scheme showing the strategy for hairpin-bisulfite PCR. ( A ) Genomic DNA sequence corresponding to the domain of the Peg3 imprinting control region analyzed in this study. CpG sites are numbered and shown in bold. The BbvI recognition/cleavage site is indicated in gray. Sequences indicated by solid arrows correspond to the modified primers (Cs substituted by Ts) used for PCR. ( B ) Diagram illustrating the ligation of the BbvI-cleavaged genomic DNA with the hairpin (labeled in gray). The resulting DNA is bisulfite-converted and subjected to PCR amplification with the oligonucleotides indicated in (A). The resulting amplification bands were cloned and Sanger sequenced (see Figure 5B and C ).

    Article Snippet: Briefly, 50 ng of DNA isolated from 4C sorted neuronal nuclei was digested with 2.5 U BbvI (Thermo Fisher Scientific) for 1 h at 37°C.

    Techniques: Polymerase Chain Reaction, Sequencing, Modification, Ligation, Labeling, Amplification, Clone Assay

    Distribution of RFLP patterns of cbbL gene fragments from the red-like cbbL gene libraries of the HKO, HSM, and HNPK soil samples. cbbL PCR products were digested with the restriction endonuclease BbvI.

    Journal: Applied and Environmental Microbiology

    Article Title: Diversity of Green-Like and Red-Like Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase Large-Subunit Genes (cbbL) in Differently Managed Agricultural Soils

    doi: 10.1128/AEM.71.1.175-184.2005

    Figure Lengend Snippet: Distribution of RFLP patterns of cbbL gene fragments from the red-like cbbL gene libraries of the HKO, HSM, and HNPK soil samples. cbbL PCR products were digested with the restriction endonuclease BbvI.

    Article Snippet: Ten microliters of each PCR product was hydrolyzed with 2 U of the restriction endonuclease BbvI (MBI Fermentas).

    Techniques: Polymerase Chain Reaction