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  • 99
    Cell Signaling Technology Inc bax
    Tanshinol inhibits the growth of HepG2 cell in a xenograft model. Notes: ( A ) A xenograft model of HCC was established using HepG2 cell. Mice in the experimental groups were administrated tanshinol intragastrically daily for 5 weeks. Control mice in vehicle-treated group received the same dose of vehicle only. Tumor volumes in each group were calculated. ( B ) Five weeks after inoculation, tumor tissues in each group were dissected from mice. ( C ) Dissected tumors were weighed. The average weight of tumors from each group of mice was calculated. ( D ) The expression of <t>Bcl-2</t> and <t>Bax</t> was detected in xenografts by IHC. Scale bar: 100 µm. ** P
    Bax, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bax/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bax - by Bioz Stars, 2021-03
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    86
    Santa Cruz Biotechnology bax
    Effect of CuNPs/TiO 2 (0, 1, 10, or 100 µg/mL) alone (white bars) and in combination with vitex ( Vitex agnus-castus , 10 µg/mL; black bars) on the viability (Trypan blue exclusion assays) ( A ), proliferation (expression of proliferating cell nuclear antigen, <t>PCNA,</t> quantitative immunocytochemistry) ( B ), apoptosis (expression of <t>bax,</t> quantitative immunocytochemistry) ( C ), and secretion of progesterone (enzyme-linked immunosorbent assay, ELISA) ( D ), testosterone (ELISA) ( E ), and 17 β -estradiol (ELISA) ( F ) in cultured porcine ovarian granulosa cells. The values are expressed as the mean ± SEM; a: effect of vitex–significant ( p
    Bax, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bax/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bax - by Bioz Stars, 2021-03
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    99
    Cell Signaling Technology Inc anti bax
    Effect of CuNPs/TiO 2 (0, 1, 10, or 100 µg/mL) alone (white bars) and in combination with vitex ( Vitex agnus-castus , 10 µg/mL; black bars) on the viability (Trypan blue exclusion assays) ( A ), proliferation (expression of proliferating cell nuclear antigen, <t>PCNA,</t> quantitative immunocytochemistry) ( B ), apoptosis (expression of <t>bax,</t> quantitative immunocytochemistry) ( C ), and secretion of progesterone (enzyme-linked immunosorbent assay, ELISA) ( D ), testosterone (ELISA) ( E ), and 17 β -estradiol (ELISA) ( F ) in cultured porcine ovarian granulosa cells. The values are expressed as the mean ± SEM; a: effect of vitex–significant ( p
    Anti Bax, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti bax/product/Cell Signaling Technology Inc
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti bax - by Bioz Stars, 2021-03
    99/100 stars
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    86
    Santa Cruz Biotechnology anti bax
    HT and OP attenuated the altered expression of GRP78, <t>CHOP,</t> Bcl2 and <t>Bax</t> induced by acrolein in H9c2 cells. ( A , B , E – H ) The expression of GRP78, CHOP, Bcl2 and Bax at the protein and mRNA levels after H9c2 cells were pretreated with HT (5 µM) for 24 h, and the combination with acrolein (20 µM) for 12 h, as measured by Western blotting and qRT-PCR. ( C , D , I – L ) The expression of GRP78, CHOP, Bcl2 and Bax at the protein and mRNA levels after H9c2 cells were pretreated with OP (20 µM) for 24 h, and the combination with acrolein (20 µM) for 12 h, as measured by Western blotting and qRT-PCR. * p
    Anti Bax, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti bax/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti bax - by Bioz Stars, 2021-03
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    N/A
    The Bax Antibody BAX 962 PE from Novus Biologicals is a mouse monoclonal antibody to Bax This antibody reacts with human monkey mouse negative rat negative The Bax Antibody BAX
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    N/A
    The Bax Antibody BAX 962 PerCP from Novus Biologicals is a mouse monoclonal antibody to Bax This antibody reacts with human monkey mouse negative rat negative The Bax Antibody BAX
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    N/A
    Category Protein Products Peptides Bax BH3L63A Size 1 mg
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    Image Search Results


    Tanshinol inhibits the growth of HepG2 cell in a xenograft model. Notes: ( A ) A xenograft model of HCC was established using HepG2 cell. Mice in the experimental groups were administrated tanshinol intragastrically daily for 5 weeks. Control mice in vehicle-treated group received the same dose of vehicle only. Tumor volumes in each group were calculated. ( B ) Five weeks after inoculation, tumor tissues in each group were dissected from mice. ( C ) Dissected tumors were weighed. The average weight of tumors from each group of mice was calculated. ( D ) The expression of Bcl-2 and Bax was detected in xenografts by IHC. Scale bar: 100 µm. ** P

    Journal: OncoTargets and therapy

    Article Title: Tanshinol inhibits the growth, migration and invasion of hepatocellular carcinoma cells via regulating the PI3K-AKT signaling pathway

    doi: 10.2147/OTT.S185997

    Figure Lengend Snippet: Tanshinol inhibits the growth of HepG2 cell in a xenograft model. Notes: ( A ) A xenograft model of HCC was established using HepG2 cell. Mice in the experimental groups were administrated tanshinol intragastrically daily for 5 weeks. Control mice in vehicle-treated group received the same dose of vehicle only. Tumor volumes in each group were calculated. ( B ) Five weeks after inoculation, tumor tissues in each group were dissected from mice. ( C ) Dissected tumors were weighed. The average weight of tumors from each group of mice was calculated. ( D ) The expression of Bcl-2 and Bax was detected in xenografts by IHC. Scale bar: 100 µm. ** P

    Article Snippet: Then, the cells were incubated with primary antibody Bcl-2 or Bax (1:100) (Cell Signaling Technology, Danvers, MA, USA) for overnight.

    Techniques: Mouse Assay, Expressing, Immunohistochemistry

    Tanshinol increases the apoptosis of HepG2 cell. Notes: ( A ) Representative images of Hoechst staining of cells treated with tanshinol. Arrows: apoptotic nuclear changes. Scale bar: 500 µm. ( B ) The apoptosis of HepG2 cell was analyzed by flow cytometry with Annexin V-FITC/PI staining. ( C ) The expression of Bax and Bcl-2 were detected using Western blotting assay. GAPDH was used as loading control. ( D ) The expression of Bcl-2 and Bax in HepG2 cell was analyzed using immunohistochemical staining. * P

    Journal: OncoTargets and therapy

    Article Title: Tanshinol inhibits the growth, migration and invasion of hepatocellular carcinoma cells via regulating the PI3K-AKT signaling pathway

    doi: 10.2147/OTT.S185997

    Figure Lengend Snippet: Tanshinol increases the apoptosis of HepG2 cell. Notes: ( A ) Representative images of Hoechst staining of cells treated with tanshinol. Arrows: apoptotic nuclear changes. Scale bar: 500 µm. ( B ) The apoptosis of HepG2 cell was analyzed by flow cytometry with Annexin V-FITC/PI staining. ( C ) The expression of Bax and Bcl-2 were detected using Western blotting assay. GAPDH was used as loading control. ( D ) The expression of Bcl-2 and Bax in HepG2 cell was analyzed using immunohistochemical staining. * P

    Article Snippet: Then, the cells were incubated with primary antibody Bcl-2 or Bax (1:100) (Cell Signaling Technology, Danvers, MA, USA) for overnight.

    Techniques: Staining, Flow Cytometry, Cytometry, Expressing, Western Blot, Immunohistochemistry

    Effect of CuNPs/TiO 2 (0, 1, 10, or 100 µg/mL) alone (white bars) and in combination with vitex ( Vitex agnus-castus , 10 µg/mL; black bars) on the viability (Trypan blue exclusion assays) ( A ), proliferation (expression of proliferating cell nuclear antigen, PCNA, quantitative immunocytochemistry) ( B ), apoptosis (expression of bax, quantitative immunocytochemistry) ( C ), and secretion of progesterone (enzyme-linked immunosorbent assay, ELISA) ( D ), testosterone (ELISA) ( E ), and 17 β -estradiol (ELISA) ( F ) in cultured porcine ovarian granulosa cells. The values are expressed as the mean ± SEM; a: effect of vitex–significant ( p

    Journal: Nanomaterials

    Article Title: Abatement of the Stimulatory Effect of Copper Nanoparticles Supported on Titania on Ovarian Cell Functions by Some Plants and Phytochemicals

    doi: 10.3390/nano10091859

    Figure Lengend Snippet: Effect of CuNPs/TiO 2 (0, 1, 10, or 100 µg/mL) alone (white bars) and in combination with vitex ( Vitex agnus-castus , 10 µg/mL; black bars) on the viability (Trypan blue exclusion assays) ( A ), proliferation (expression of proliferating cell nuclear antigen, PCNA, quantitative immunocytochemistry) ( B ), apoptosis (expression of bax, quantitative immunocytochemistry) ( C ), and secretion of progesterone (enzyme-linked immunosorbent assay, ELISA) ( D ), testosterone (ELISA) ( E ), and 17 β -estradiol (ELISA) ( F ) in cultured porcine ovarian granulosa cells. The values are expressed as the mean ± SEM; a: effect of vitex–significant ( p

    Article Snippet: Immunocytochemical Analysis of Proliferation and Apoptosis MarkersImmunocytochemistry was used to detect the presence of PCNA and bax in the cells, as described previously [ , , , , , , ], by means of primary monoclonal antibodies against PCNA and bax (dilution 1:500; from Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), secondary swine antibodies against mouse IgG labeled with horseradish peroxidase (dilution 1:1000; Servac, Prague, Czech Republic) and visualized by DAB-substrate staining (Roche Diagnostics GmbH, Manheim, Germany).

    Techniques: Expressing, Immunocytochemistry, Enzyme-linked Immunosorbent Assay, Cell Culture

    Effect of CuNPs/TiO 2 (0, 1, 10, or 100 µg/mL) alone (white bars) and in combination with rutin (10 µg/mL; black bars) on the viability (Trypan blue exclusion assays) ( A ), proliferation (expression of proliferating cell nuclear antigen, PCNA, quantitative immunocytochemistry) ( B ), apoptosis (expression of bax, quantitative immunocytochemistry) ( C ), and secretion of progesterone (enzyme-linked immunosorbent assay, ELISA) ( D ), testosterone (ELISA) ( E ), and 17 β -estradiol (ELISA) ( F ) in cultured porcine ovarian granulosa cells. The values are expressed as the mean ± SEM; a: effect of rutin–significant ( p

    Journal: Nanomaterials

    Article Title: Abatement of the Stimulatory Effect of Copper Nanoparticles Supported on Titania on Ovarian Cell Functions by Some Plants and Phytochemicals

    doi: 10.3390/nano10091859

    Figure Lengend Snippet: Effect of CuNPs/TiO 2 (0, 1, 10, or 100 µg/mL) alone (white bars) and in combination with rutin (10 µg/mL; black bars) on the viability (Trypan blue exclusion assays) ( A ), proliferation (expression of proliferating cell nuclear antigen, PCNA, quantitative immunocytochemistry) ( B ), apoptosis (expression of bax, quantitative immunocytochemistry) ( C ), and secretion of progesterone (enzyme-linked immunosorbent assay, ELISA) ( D ), testosterone (ELISA) ( E ), and 17 β -estradiol (ELISA) ( F ) in cultured porcine ovarian granulosa cells. The values are expressed as the mean ± SEM; a: effect of rutin–significant ( p

    Article Snippet: Immunocytochemical Analysis of Proliferation and Apoptosis MarkersImmunocytochemistry was used to detect the presence of PCNA and bax in the cells, as described previously [ , , , , , , ], by means of primary monoclonal antibodies against PCNA and bax (dilution 1:500; from Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), secondary swine antibodies against mouse IgG labeled with horseradish peroxidase (dilution 1:1000; Servac, Prague, Czech Republic) and visualized by DAB-substrate staining (Roche Diagnostics GmbH, Manheim, Germany).

    Techniques: Expressing, Immunocytochemistry, Enzyme-linked Immunosorbent Assay, Cell Culture

    Effect of CuNPs/TiO 2 (0, 1, 10, or 100 µg/mL) alone (white bars) and in combination with apigenin (10 µg/mL; black bars) on the viability (Trypan blue exclusion assays) ( A ), proliferation (expression of proliferating cell nuclear antigen, PCNA, quantitative immunocytochemistry) ( B ), apoptosis (expression of bax, quantitative immunocytochemistry) ( C ), and secretion of progesterone (enzyme-linked immunosorbent assay, ELISA) ( D ), testosterone (ELISA) ( E ), and 17 β -estradiol (ELISA) ( F ) in cultured porcine ovarian granulosa cells. The values are expressed as the mean ± SEM; a: effect of apigenin–significant ( p

    Journal: Nanomaterials

    Article Title: Abatement of the Stimulatory Effect of Copper Nanoparticles Supported on Titania on Ovarian Cell Functions by Some Plants and Phytochemicals

    doi: 10.3390/nano10091859

    Figure Lengend Snippet: Effect of CuNPs/TiO 2 (0, 1, 10, or 100 µg/mL) alone (white bars) and in combination with apigenin (10 µg/mL; black bars) on the viability (Trypan blue exclusion assays) ( A ), proliferation (expression of proliferating cell nuclear antigen, PCNA, quantitative immunocytochemistry) ( B ), apoptosis (expression of bax, quantitative immunocytochemistry) ( C ), and secretion of progesterone (enzyme-linked immunosorbent assay, ELISA) ( D ), testosterone (ELISA) ( E ), and 17 β -estradiol (ELISA) ( F ) in cultured porcine ovarian granulosa cells. The values are expressed as the mean ± SEM; a: effect of apigenin–significant ( p

    Article Snippet: Immunocytochemical Analysis of Proliferation and Apoptosis MarkersImmunocytochemistry was used to detect the presence of PCNA and bax in the cells, as described previously [ , , , , , , ], by means of primary monoclonal antibodies against PCNA and bax (dilution 1:500; from Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), secondary swine antibodies against mouse IgG labeled with horseradish peroxidase (dilution 1:1000; Servac, Prague, Czech Republic) and visualized by DAB-substrate staining (Roche Diagnostics GmbH, Manheim, Germany).

    Techniques: Expressing, Immunocytochemistry, Enzyme-linked Immunosorbent Assay, Cell Culture

    Effect of CuNPs/TiO 2 (0, 1, 10, or 100 µg/mL) alone (white bars) and in combination with buckwheat ( Fagopyrum esculentum , 10 µg/mL; black bars) on the viability (Trypan blue exclusion assays) ( A ), proliferation (expression of proliferating cell nuclear antigen, PCNA, quantitative immunocytochemistry) ( B ), apoptosis (expression of bax, quantitative immunocytochemistry) ( C ), and secretion of progesterone (enzyme-linked immunosorbent assay, ELISA) ( D ), testosterone (ELISA) ( E ), and 17 β -estradiol (ELISA) ( F ) in cultured porcine ovarian granulosa cells. The values are expressed as the mean ± SEM; a: effect of buckwheat–significant ( p

    Journal: Nanomaterials

    Article Title: Abatement of the Stimulatory Effect of Copper Nanoparticles Supported on Titania on Ovarian Cell Functions by Some Plants and Phytochemicals

    doi: 10.3390/nano10091859

    Figure Lengend Snippet: Effect of CuNPs/TiO 2 (0, 1, 10, or 100 µg/mL) alone (white bars) and in combination with buckwheat ( Fagopyrum esculentum , 10 µg/mL; black bars) on the viability (Trypan blue exclusion assays) ( A ), proliferation (expression of proliferating cell nuclear antigen, PCNA, quantitative immunocytochemistry) ( B ), apoptosis (expression of bax, quantitative immunocytochemistry) ( C ), and secretion of progesterone (enzyme-linked immunosorbent assay, ELISA) ( D ), testosterone (ELISA) ( E ), and 17 β -estradiol (ELISA) ( F ) in cultured porcine ovarian granulosa cells. The values are expressed as the mean ± SEM; a: effect of buckwheat–significant ( p

    Article Snippet: Immunocytochemical Analysis of Proliferation and Apoptosis MarkersImmunocytochemistry was used to detect the presence of PCNA and bax in the cells, as described previously [ , , , , , , ], by means of primary monoclonal antibodies against PCNA and bax (dilution 1:500; from Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA), secondary swine antibodies against mouse IgG labeled with horseradish peroxidase (dilution 1:1000; Servac, Prague, Czech Republic) and visualized by DAB-substrate staining (Roche Diagnostics GmbH, Manheim, Germany).

    Techniques: Expressing, Immunocytochemistry, Enzyme-linked Immunosorbent Assay, Cell Culture

    HT and OP attenuated the altered expression of GRP78, CHOP, Bcl2 and Bax induced by acrolein in H9c2 cells. ( A , B , E – H ) The expression of GRP78, CHOP, Bcl2 and Bax at the protein and mRNA levels after H9c2 cells were pretreated with HT (5 µM) for 24 h, and the combination with acrolein (20 µM) for 12 h, as measured by Western blotting and qRT-PCR. ( C , D , I – L ) The expression of GRP78, CHOP, Bcl2 and Bax at the protein and mRNA levels after H9c2 cells were pretreated with OP (20 µM) for 24 h, and the combination with acrolein (20 µM) for 12 h, as measured by Western blotting and qRT-PCR. * p

    Journal: International Journal of Molecular Sciences

    Article Title: Protective Effects of Olive Leaf Extract on Acrolein-Exacerbated Myocardial Infarction via an Endoplasmic Reticulum Stress Pathway

    doi: 10.3390/ijms19020493

    Figure Lengend Snippet: HT and OP attenuated the altered expression of GRP78, CHOP, Bcl2 and Bax induced by acrolein in H9c2 cells. ( A , B , E – H ) The expression of GRP78, CHOP, Bcl2 and Bax at the protein and mRNA levels after H9c2 cells were pretreated with HT (5 µM) for 24 h, and the combination with acrolein (20 µM) for 12 h, as measured by Western blotting and qRT-PCR. ( C , D , I – L ) The expression of GRP78, CHOP, Bcl2 and Bax at the protein and mRNA levels after H9c2 cells were pretreated with OP (20 µM) for 24 h, and the combination with acrolein (20 µM) for 12 h, as measured by Western blotting and qRT-PCR. * p

    Article Snippet: The primary antibodies included: anti-GRP78 (1:1000, proteintech, Wuhan, China), anti-CHOP and anti-F4/80 (1:1000, Cell Signaling Technology, Danvers, MA, USA), anti-Bcl2 and anti-Bax (1:500, Santa Cruz Biotechnology, Dallas, TX, USA), anti-β actin (1:1000, BOSTER, Wuhan, China), anti-GAPDH (1:1000, Beyotime, Shanghai, China).

    Techniques: Expressing, Western Blot, Quantitative RT-PCR

    Effect of OLE on myocardium protection through the pathway of ERS, Bcl2/Bax in rats. ( A , B ) The protein expression of GRP78, CHOP, Bcl2 and Bax in different groups, as detected by Western blotting; ( C – F ) The mRNA expression of GRP78, CHOP, Bcl2 and Bax in different groups, as detected by qRT-PCR. * p

    Journal: International Journal of Molecular Sciences

    Article Title: Protective Effects of Olive Leaf Extract on Acrolein-Exacerbated Myocardial Infarction via an Endoplasmic Reticulum Stress Pathway

    doi: 10.3390/ijms19020493

    Figure Lengend Snippet: Effect of OLE on myocardium protection through the pathway of ERS, Bcl2/Bax in rats. ( A , B ) The protein expression of GRP78, CHOP, Bcl2 and Bax in different groups, as detected by Western blotting; ( C – F ) The mRNA expression of GRP78, CHOP, Bcl2 and Bax in different groups, as detected by qRT-PCR. * p

    Article Snippet: The primary antibodies included: anti-GRP78 (1:1000, proteintech, Wuhan, China), anti-CHOP and anti-F4/80 (1:1000, Cell Signaling Technology, Danvers, MA, USA), anti-Bcl2 and anti-Bax (1:500, Santa Cruz Biotechnology, Dallas, TX, USA), anti-β actin (1:1000, BOSTER, Wuhan, China), anti-GAPDH (1:1000, Beyotime, Shanghai, China).

    Techniques: Expressing, Western Blot, Quantitative RT-PCR

    Effect of OLE on myocardial apoptosis and infiltration of macrophages induced by acrolein. ( A , B ) Representative photographs of heart sections with immunohistochemical staining of Bax and F4/80 (400×) in different groups.

    Journal: International Journal of Molecular Sciences

    Article Title: Protective Effects of Olive Leaf Extract on Acrolein-Exacerbated Myocardial Infarction via an Endoplasmic Reticulum Stress Pathway

    doi: 10.3390/ijms19020493

    Figure Lengend Snippet: Effect of OLE on myocardial apoptosis and infiltration of macrophages induced by acrolein. ( A , B ) Representative photographs of heart sections with immunohistochemical staining of Bax and F4/80 (400×) in different groups.

    Article Snippet: The primary antibodies included: anti-GRP78 (1:1000, proteintech, Wuhan, China), anti-CHOP and anti-F4/80 (1:1000, Cell Signaling Technology, Danvers, MA, USA), anti-Bcl2 and anti-Bax (1:500, Santa Cruz Biotechnology, Dallas, TX, USA), anti-β actin (1:1000, BOSTER, Wuhan, China), anti-GAPDH (1:1000, Beyotime, Shanghai, China).

    Techniques: Immunohistochemistry, Staining

    Acrolein altered the expression of GRP78, CHOP, Bcl2 and Bax in H9c2 cells. ( A , C , E , F , H , I ) The expression of GRP78, CHOP, Bcl2 and Bax at the protein and mRNA levels after H9c2 cells were treated with acrolein (20 µM) for the indicated time points (0, 3, 6, 12, 24 h), as measured by Western blotting and qRT-PCR; ( B , D , G , H , J , K ) The expression of GRP78, CHOP, Bcl2 and Bax at the protein and mRNA levels after the H9c2 cells were treated with acrolein (0, 5, 10, 20, 40 µM) for 12 h, as measured by Western blotting and qRT-PCR. * p

    Journal: International Journal of Molecular Sciences

    Article Title: Protective Effects of Olive Leaf Extract on Acrolein-Exacerbated Myocardial Infarction via an Endoplasmic Reticulum Stress Pathway

    doi: 10.3390/ijms19020493

    Figure Lengend Snippet: Acrolein altered the expression of GRP78, CHOP, Bcl2 and Bax in H9c2 cells. ( A , C , E , F , H , I ) The expression of GRP78, CHOP, Bcl2 and Bax at the protein and mRNA levels after H9c2 cells were treated with acrolein (20 µM) for the indicated time points (0, 3, 6, 12, 24 h), as measured by Western blotting and qRT-PCR; ( B , D , G , H , J , K ) The expression of GRP78, CHOP, Bcl2 and Bax at the protein and mRNA levels after the H9c2 cells were treated with acrolein (0, 5, 10, 20, 40 µM) for 12 h, as measured by Western blotting and qRT-PCR. * p

    Article Snippet: The primary antibodies included: anti-GRP78 (1:1000, proteintech, Wuhan, China), anti-CHOP and anti-F4/80 (1:1000, Cell Signaling Technology, Danvers, MA, USA), anti-Bcl2 and anti-Bax (1:500, Santa Cruz Biotechnology, Dallas, TX, USA), anti-β actin (1:1000, BOSTER, Wuhan, China), anti-GAPDH (1:1000, Beyotime, Shanghai, China).

    Techniques: Expressing, Western Blot, Quantitative RT-PCR