Journal: International Journal of Molecular Sciences
Article Title: Protective Effects of Olive Leaf Extract on Acrolein-Exacerbated Myocardial Infarction via an Endoplasmic Reticulum Stress Pathway
Figure Lengend Snippet: Acrolein altered the expression of GRP78, CHOP, Bcl2 and Bax in H9c2 cells. ( A , C , E , F , H , I ) The expression of GRP78, CHOP, Bcl2 and Bax at the protein and mRNA levels after H9c2 cells were treated with acrolein (20 µM) for the indicated time points (0, 3, 6, 12, 24 h), as measured by Western blotting and qRT-PCR; ( B , D , G , H , J , K ) The expression of GRP78, CHOP, Bcl2 and Bax at the protein and mRNA levels after the H9c2 cells were treated with acrolein (0, 5, 10, 20, 40 µM) for 12 h, as measured by Western blotting and qRT-PCR. * p
Article Snippet: The primary antibodies included: anti-GRP78 (1:1000, proteintech, Wuhan, China), anti-CHOP and anti-F4/80 (1:1000, Cell Signaling Technology, Danvers, MA, USA), anti-Bcl2 and anti-Bax (1:500, Santa Cruz Biotechnology, Dallas, TX, USA), anti-β actin (1:1000, BOSTER, Wuhan, China), anti-GAPDH (1:1000, Beyotime, Shanghai, China).
Techniques: Expressing, Western Blot, Quantitative RT-PCR