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    Millipore bavachin
    The effect of <t>bavachin</t> on insulin and AMPK signaling pathway-mediated glucose uptake: ( A ) the effect of bavachin on insulin-stimulated glucose uptake in differentiated adipocytes and myoblasts. Differentiated 3T3-L1 adipocytes ( left panel) and C2C12 myoblasts ( right panel) were serum-starved and then exposed to bavachin (0.5, 2 and 10 µM for adipocytes, 1, 5 and 10 nM for myoblasts) for 24 h. After 1 h incubation of insulin (100 nM), the cultures were incubated with 2-( N -(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino)-2-deoxyglucose (2-NBDG) labeling-medium for 1 h. Fluorescence retained in the cell monolayer was measured with excitation wavelength at 465 nm and emission wavelength at 540 nm. Data are expressed as mean ± SD. # p
    Bavachin, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bavachin/product/Millipore
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bavachin - by Bioz Stars, 2022-08
    88/100 stars
      Buy from Supplier

    93
    MedChemExpress bavachin
    <t>Bavachin</t> induces GSH depletion and lipid peroxidation accumulation in osteosarcoma (OS) cells. (a, b) Bavachin induces GSH depletion in a dose-related manner over 24 h. (c, d) Bavachin greatly induces ROS generation in OS cells over 24 h. (e, f) Bavachin increases MDA accumulation in a dose-related manner. (g, h) Western blotting analysis shows the expression of p-STAT3, STAT3, P53, SLC7A11, and GPX4 in OS cells treated with bavachin (10 μ M, 20 μ M, and 40 μ M) for 24 h. p
    Bavachin, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bavachin/product/MedChemExpress
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bavachin - by Bioz Stars, 2022-08
    93/100 stars
      Buy from Supplier

    Image Search Results


    The effect of bavachin on insulin and AMPK signaling pathway-mediated glucose uptake: ( A ) the effect of bavachin on insulin-stimulated glucose uptake in differentiated adipocytes and myoblasts. Differentiated 3T3-L1 adipocytes ( left panel) and C2C12 myoblasts ( right panel) were serum-starved and then exposed to bavachin (0.5, 2 and 10 µM for adipocytes, 1, 5 and 10 nM for myoblasts) for 24 h. After 1 h incubation of insulin (100 nM), the cultures were incubated with 2-( N -(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino)-2-deoxyglucose (2-NBDG) labeling-medium for 1 h. Fluorescence retained in the cell monolayer was measured with excitation wavelength at 465 nm and emission wavelength at 540 nm. Data are expressed as mean ± SD. # p

    Journal: International Journal of Molecular Sciences

    Article Title: Bavachin from Psoralea corylifolia Improves Insulin-Dependent Glucose Uptake through Insulin Signaling and AMPK Activation in 3T3-L1 Adipocytes

    doi: 10.3390/ijms17040527

    Figure Lengend Snippet: The effect of bavachin on insulin and AMPK signaling pathway-mediated glucose uptake: ( A ) the effect of bavachin on insulin-stimulated glucose uptake in differentiated adipocytes and myoblasts. Differentiated 3T3-L1 adipocytes ( left panel) and C2C12 myoblasts ( right panel) were serum-starved and then exposed to bavachin (0.5, 2 and 10 µM for adipocytes, 1, 5 and 10 nM for myoblasts) for 24 h. After 1 h incubation of insulin (100 nM), the cultures were incubated with 2-( N -(7-nitrobenz-2-oxa-1,3-diazol-4-yl) amino)-2-deoxyglucose (2-NBDG) labeling-medium for 1 h. Fluorescence retained in the cell monolayer was measured with excitation wavelength at 465 nm and emission wavelength at 540 nm. Data are expressed as mean ± SD. # p

    Article Snippet: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) and Cell Proliferation Assay To determine the effect of bavachin on pre-adipocyte viability, 3T3-L1 pre-adipocytes were incubated with different concentration of bavachin for 72 h. The cells were subjected to MTT assay (Sigma).

    Techniques: Incubation, Labeling, Fluorescence

    Effect of bavachin on proliferation and differentiation of adipocytes: ( A ) 3T3-L1 cells were differentiated with 0.5, 2 and 10 µM bavachin. Lipid accumulation was quantified by measuring absorbance of ORO staining. Images of adipocytes stained with ORO ( upper panel, magnification, 40×) and differentiated adipocytes ( lower panel, magnification, 100×) were visualized by light microscopy. Scale bar = 50 μm. Data are expressed as mean ± SD. # p

    Journal: International Journal of Molecular Sciences

    Article Title: Bavachin from Psoralea corylifolia Improves Insulin-Dependent Glucose Uptake through Insulin Signaling and AMPK Activation in 3T3-L1 Adipocytes

    doi: 10.3390/ijms17040527

    Figure Lengend Snippet: Effect of bavachin on proliferation and differentiation of adipocytes: ( A ) 3T3-L1 cells were differentiated with 0.5, 2 and 10 µM bavachin. Lipid accumulation was quantified by measuring absorbance of ORO staining. Images of adipocytes stained with ORO ( upper panel, magnification, 40×) and differentiated adipocytes ( lower panel, magnification, 100×) were visualized by light microscopy. Scale bar = 50 μm. Data are expressed as mean ± SD. # p

    Article Snippet: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) and Cell Proliferation Assay To determine the effect of bavachin on pre-adipocyte viability, 3T3-L1 pre-adipocytes were incubated with different concentration of bavachin for 72 h. The cells were subjected to MTT assay (Sigma).

    Techniques: Staining, Light Microscopy

    Effect of bavachin on the expression of adipogenic factors: ( A ) Effect of bavachin on PPARγ and C/EBPα protein levels of differentiated adipocytes. Pre-adipocytes were differentiated with or without bavachin at indicated concentrations. Cells were harvested on differentiation Day 5 (D5) and protein extracts (30 µg) were analyzed for PPARγ and C/EBPα expression by Western blot analysis. Actin was used as a loading control; ( B ) Effect of bavachin on the mRNA expression of PPARγ and C/EBPα. Differentiated adipocytes were harvested and lysed on D5. Gene expression levels of PPARγ and C/EBPα were determined by quantitative real-time polymerase chain reaction (qPCR); ( C ) Effect of bavachin on transactivation of PPARγ. PPARγ transactivation is presented as relative luciferase activity as described above; ( D ) Effect of bavachin on gene expression and secretion of adiponectin in differentiated adipocytes determined by real time RT-PCR and ELISA, respectively, as mentioned in the Materials and Methods. Data are expressed as mean ± SD. # p

    Journal: International Journal of Molecular Sciences

    Article Title: Bavachin from Psoralea corylifolia Improves Insulin-Dependent Glucose Uptake through Insulin Signaling and AMPK Activation in 3T3-L1 Adipocytes

    doi: 10.3390/ijms17040527

    Figure Lengend Snippet: Effect of bavachin on the expression of adipogenic factors: ( A ) Effect of bavachin on PPARγ and C/EBPα protein levels of differentiated adipocytes. Pre-adipocytes were differentiated with or without bavachin at indicated concentrations. Cells were harvested on differentiation Day 5 (D5) and protein extracts (30 µg) were analyzed for PPARγ and C/EBPα expression by Western blot analysis. Actin was used as a loading control; ( B ) Effect of bavachin on the mRNA expression of PPARγ and C/EBPα. Differentiated adipocytes were harvested and lysed on D5. Gene expression levels of PPARγ and C/EBPα were determined by quantitative real-time polymerase chain reaction (qPCR); ( C ) Effect of bavachin on transactivation of PPARγ. PPARγ transactivation is presented as relative luciferase activity as described above; ( D ) Effect of bavachin on gene expression and secretion of adiponectin in differentiated adipocytes determined by real time RT-PCR and ELISA, respectively, as mentioned in the Materials and Methods. Data are expressed as mean ± SD. # p

    Article Snippet: 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide (MTT) and Cell Proliferation Assay To determine the effect of bavachin on pre-adipocyte viability, 3T3-L1 pre-adipocytes were incubated with different concentration of bavachin for 72 h. The cells were subjected to MTT assay (Sigma).

    Techniques: Expressing, Western Blot, Real-time Polymerase Chain Reaction, Luciferase, Activity Assay, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay

    Bavachin induces GSH depletion and lipid peroxidation accumulation in osteosarcoma (OS) cells. (a, b) Bavachin induces GSH depletion in a dose-related manner over 24 h. (c, d) Bavachin greatly induces ROS generation in OS cells over 24 h. (e, f) Bavachin increases MDA accumulation in a dose-related manner. (g, h) Western blotting analysis shows the expression of p-STAT3, STAT3, P53, SLC7A11, and GPX4 in OS cells treated with bavachin (10 μ M, 20 μ M, and 40 μ M) for 24 h. p

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Bavachin Induces Ferroptosis through the STAT3/P53/SLC7A11 Axis in Osteosarcoma Cells

    doi: 10.1155/2021/1783485

    Figure Lengend Snippet: Bavachin induces GSH depletion and lipid peroxidation accumulation in osteosarcoma (OS) cells. (a, b) Bavachin induces GSH depletion in a dose-related manner over 24 h. (c, d) Bavachin greatly induces ROS generation in OS cells over 24 h. (e, f) Bavachin increases MDA accumulation in a dose-related manner. (g, h) Western blotting analysis shows the expression of p-STAT3, STAT3, P53, SLC7A11, and GPX4 in OS cells treated with bavachin (10 μ M, 20 μ M, and 40 μ M) for 24 h. p

    Article Snippet: Bavachin was purchased from MCE (China), and pifithrin-α (PFT-α), deferoxamine (DFO), ferrostatin-1 (Fer-1), and liproxstatin-1 (Lip-1) were purchased from Topscience (China).

    Techniques: Multiple Displacement Amplification, Western Blot, Expressing

    SLC7A11 overexpression alleviates bavachin-induced ferroptosis in osteosarcoma (OS) cells. OS cells were transfected with a SLC7A11 overexpressing plasmid and treated with bavachin (40 μ M, 24 h). (a, b) Western blotting demonstrates the relative levels of SLC7A11 and GPX4 protein expression. (c) Overexpression of SLC7A11 restores bavachin-induced GSH depletion in OS cells. (d, e) Overexpression of SLC7A11 reduces bavachin-induced ROS and MDA accumulation. (f) Overexpression of SLC7A11 recovers bavachin-induced ferroptosis in OS cells. ∗ p

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Bavachin Induces Ferroptosis through the STAT3/P53/SLC7A11 Axis in Osteosarcoma Cells

    doi: 10.1155/2021/1783485

    Figure Lengend Snippet: SLC7A11 overexpression alleviates bavachin-induced ferroptosis in osteosarcoma (OS) cells. OS cells were transfected with a SLC7A11 overexpressing plasmid and treated with bavachin (40 μ M, 24 h). (a, b) Western blotting demonstrates the relative levels of SLC7A11 and GPX4 protein expression. (c) Overexpression of SLC7A11 restores bavachin-induced GSH depletion in OS cells. (d, e) Overexpression of SLC7A11 reduces bavachin-induced ROS and MDA accumulation. (f) Overexpression of SLC7A11 recovers bavachin-induced ferroptosis in OS cells. ∗ p

    Article Snippet: Bavachin was purchased from MCE (China), and pifithrin-α (PFT-α), deferoxamine (DFO), ferrostatin-1 (Fer-1), and liproxstatin-1 (Lip-1) were purchased from Topscience (China).

    Techniques: Over Expression, Transfection, Plasmid Preparation, Western Blot, Expressing, Multiple Displacement Amplification

    Bavachin-induced ferrous iron accumulation initiates death of osteosarcoma (OS) cells. (a) Bavachin (24 h) induced ferrous iron accumulation in OS cells occurs in a concentration-dependent manner. (b) Deferoxamine (DFO) rescues cells from bavachin-induced cell death. (c, d) Western blotting analysis shows the protein levels of transferrin receptor (TFRC), divalent metal transporter-1 (DMT1), ferritin light chain (FTH), and ferritin heavy chain (FTL) in MG63 and HOS cells treated with bavachin (10 μ M, 20 μ M, and 40 μ M) for 24 h. ∗ p

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Bavachin Induces Ferroptosis through the STAT3/P53/SLC7A11 Axis in Osteosarcoma Cells

    doi: 10.1155/2021/1783485

    Figure Lengend Snippet: Bavachin-induced ferrous iron accumulation initiates death of osteosarcoma (OS) cells. (a) Bavachin (24 h) induced ferrous iron accumulation in OS cells occurs in a concentration-dependent manner. (b) Deferoxamine (DFO) rescues cells from bavachin-induced cell death. (c, d) Western blotting analysis shows the protein levels of transferrin receptor (TFRC), divalent metal transporter-1 (DMT1), ferritin light chain (FTH), and ferritin heavy chain (FTL) in MG63 and HOS cells treated with bavachin (10 μ M, 20 μ M, and 40 μ M) for 24 h. ∗ p

    Article Snippet: Bavachin was purchased from MCE (China), and pifithrin-α (PFT-α), deferoxamine (DFO), ferrostatin-1 (Fer-1), and liproxstatin-1 (Lip-1) were purchased from Topscience (China).

    Techniques: Concentration Assay, Western Blot

    p-STAT3 activation downregulates P53 expression to rescue bavachin-induced ferroptosis in osteosarcoma (OS) cells. (a, b) Western blotting shows the relative levels of p-STAT3, STAT3, P53, and GPX4 protein expression. (c) p-STAT3 activation restores bavachin-induced GSH depletion. (d, e) p-STAT3 activation reduces bavachin-induced ROS and MDA accumulation. (f) p-STAT3 activation rescues bavachin-induced ferroptosis in OS cells. ∗ p

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Bavachin Induces Ferroptosis through the STAT3/P53/SLC7A11 Axis in Osteosarcoma Cells

    doi: 10.1155/2021/1783485

    Figure Lengend Snippet: p-STAT3 activation downregulates P53 expression to rescue bavachin-induced ferroptosis in osteosarcoma (OS) cells. (a, b) Western blotting shows the relative levels of p-STAT3, STAT3, P53, and GPX4 protein expression. (c) p-STAT3 activation restores bavachin-induced GSH depletion. (d, e) p-STAT3 activation reduces bavachin-induced ROS and MDA accumulation. (f) p-STAT3 activation rescues bavachin-induced ferroptosis in OS cells. ∗ p

    Article Snippet: Bavachin was purchased from MCE (China), and pifithrin-α (PFT-α), deferoxamine (DFO), ferrostatin-1 (Fer-1), and liproxstatin-1 (Lip-1) were purchased from Topscience (China).

    Techniques: Activation Assay, Expressing, Western Blot, Multiple Displacement Amplification

    Schematic diagram displays potential mechanism of bavachin-induced ferroptosis in osteosarcoma cells. LP: lipid peroxidation.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Bavachin Induces Ferroptosis through the STAT3/P53/SLC7A11 Axis in Osteosarcoma Cells

    doi: 10.1155/2021/1783485

    Figure Lengend Snippet: Schematic diagram displays potential mechanism of bavachin-induced ferroptosis in osteosarcoma cells. LP: lipid peroxidation.

    Article Snippet: Bavachin was purchased from MCE (China), and pifithrin-α (PFT-α), deferoxamine (DFO), ferrostatin-1 (Fer-1), and liproxstatin-1 (Lip-1) were purchased from Topscience (China).

    Techniques:

    Bavachin induces ferroptosis in osteosarcoma (OS) cells. (a) The ultrastructure of MG63 and HOS cells was observed using transmission electronic microscopy. Cells in the bavachin (40 μ M, 24 h) group display shrinking mitochondria, higher density of the mitochondrial membrane, and reduction or disappearance of the mitochondrial cristae. (b) Merged images of JC-1 staining demonstrate the change in the mitochondrial membrane potential (MMP) (red: aggregates and high MMP; green: monomers and low MMP). (c) CCK8 assay shows that deferoxamine (DFO), ferrostatin-1 (Fer-1), and liproxstatin-1 (Lip-1) rescue bavachin-induced cell death in OS cells. ∗∗∗ p

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Bavachin Induces Ferroptosis through the STAT3/P53/SLC7A11 Axis in Osteosarcoma Cells

    doi: 10.1155/2021/1783485

    Figure Lengend Snippet: Bavachin induces ferroptosis in osteosarcoma (OS) cells. (a) The ultrastructure of MG63 and HOS cells was observed using transmission electronic microscopy. Cells in the bavachin (40 μ M, 24 h) group display shrinking mitochondria, higher density of the mitochondrial membrane, and reduction or disappearance of the mitochondrial cristae. (b) Merged images of JC-1 staining demonstrate the change in the mitochondrial membrane potential (MMP) (red: aggregates and high MMP; green: monomers and low MMP). (c) CCK8 assay shows that deferoxamine (DFO), ferrostatin-1 (Fer-1), and liproxstatin-1 (Lip-1) rescue bavachin-induced cell death in OS cells. ∗∗∗ p

    Article Snippet: Bavachin was purchased from MCE (China), and pifithrin-α (PFT-α), deferoxamine (DFO), ferrostatin-1 (Fer-1), and liproxstatin-1 (Lip-1) were purchased from Topscience (China).

    Techniques: Transmission Assay, Microscopy, Staining, CCK-8 Assay

    Bavachin inhibits osteosarcoma (OS) cell viability. (a) Chemical structure of bavachin. (b) CCK8 assay shows bavachin inhibits OS cells in a concentration- (5 μ M, 10 μ M, 20 μ M, 40 μ M, and 80 μ M) and time- (24 h, 48 h, and 72 h) dependent manner. (c) Morphologic features of OS cells on microscopy. Cells became round and showed shrunk after bavachin (10 μ M, 20 μ M, and 40 μ M) treatment for 24 h.

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Bavachin Induces Ferroptosis through the STAT3/P53/SLC7A11 Axis in Osteosarcoma Cells

    doi: 10.1155/2021/1783485

    Figure Lengend Snippet: Bavachin inhibits osteosarcoma (OS) cell viability. (a) Chemical structure of bavachin. (b) CCK8 assay shows bavachin inhibits OS cells in a concentration- (5 μ M, 10 μ M, 20 μ M, 40 μ M, and 80 μ M) and time- (24 h, 48 h, and 72 h) dependent manner. (c) Morphologic features of OS cells on microscopy. Cells became round and showed shrunk after bavachin (10 μ M, 20 μ M, and 40 μ M) treatment for 24 h.

    Article Snippet: Bavachin was purchased from MCE (China), and pifithrin-α (PFT-α), deferoxamine (DFO), ferrostatin-1 (Fer-1), and liproxstatin-1 (Lip-1) were purchased from Topscience (China).

    Techniques: CCK-8 Assay, Concentration Assay, Microscopy

    P53 inactivation upregulates SLC7A11 to alleviate bavachin-induced ferroptosis in osteosarcoma (OS) cells. OS cells were pretreated with pifithrin- α (PFT- α , 5 μ M) followed by treatment with bavachin (40 μ M, 24 h). (a, b) Western blotting shows the relative levels of P53, SLC7A11, and GPX4 protein expression. (c) PFT- α (5 μ M) restores bavachin-induced GSH depletion. (d, e) PFT- α reduces bavachin-induced ROS and MDA accumulation. (f) PFT- α recovers bavachin-induced ferroptosis in OS cells. ∗ p

    Journal: Oxidative Medicine and Cellular Longevity

    Article Title: Bavachin Induces Ferroptosis through the STAT3/P53/SLC7A11 Axis in Osteosarcoma Cells

    doi: 10.1155/2021/1783485

    Figure Lengend Snippet: P53 inactivation upregulates SLC7A11 to alleviate bavachin-induced ferroptosis in osteosarcoma (OS) cells. OS cells were pretreated with pifithrin- α (PFT- α , 5 μ M) followed by treatment with bavachin (40 μ M, 24 h). (a, b) Western blotting shows the relative levels of P53, SLC7A11, and GPX4 protein expression. (c) PFT- α (5 μ M) restores bavachin-induced GSH depletion. (d, e) PFT- α reduces bavachin-induced ROS and MDA accumulation. (f) PFT- α recovers bavachin-induced ferroptosis in OS cells. ∗ p

    Article Snippet: Bavachin was purchased from MCE (China), and pifithrin-α (PFT-α), deferoxamine (DFO), ferrostatin-1 (Fer-1), and liproxstatin-1 (Lip-1) were purchased from Topscience (China).

    Techniques: Western Blot, Expressing, Multiple Displacement Amplification