bamhi restriction enzymes Promega Search Results


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  • 91
    Promega restriction enzymes bamhi
    Determination of copy number by Southern blot. A: EgDEF1 gene; B: gypsy retroelement. One L2T-related genotype (left: LMC343 or LMC51) is compared to one non-L2T-related genotype (right: FC2317 or FC2318). Lowercase letters signal the hybridizing bands that are predicted by the in silico digestion of the Eg133H20 BAC sequence with the appropriate restriction enzyme (a: 4,069 bp <t>EcoRI</t> fragment; b: 4,841 bp HindIII fragment; c: 6,251 <t>BamHI</t> fragment; d: 6,138 bp HindIII fragment; e: 13,132 bp BamHI fragment), asterisks indicate supernumerary bands.
    Restriction Enzymes Bamhi, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 327 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Promega ndei bamhi restriction enzymes
    Determination of copy number by Southern blot. A: EgDEF1 gene; B: gypsy retroelement. One L2T-related genotype (left: LMC343 or LMC51) is compared to one non-L2T-related genotype (right: FC2317 or FC2318). Lowercase letters signal the hybridizing bands that are predicted by the in silico digestion of the Eg133H20 BAC sequence with the appropriate restriction enzyme (a: 4,069 bp <t>EcoRI</t> fragment; b: 4,841 bp HindIII fragment; c: 6,251 <t>BamHI</t> fragment; d: 6,138 bp HindIII fragment; e: 13,132 bp BamHI fragment), asterisks indicate supernumerary bands.
    Ndei Bamhi Restriction Enzymes, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 135 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Promega restriction enzyme bamhi
    Determination of copy number by Southern blot. A: EgDEF1 gene; B: gypsy retroelement. One L2T-related genotype (left: LMC343 or LMC51) is compared to one non-L2T-related genotype (right: FC2317 or FC2318). Lowercase letters signal the hybridizing bands that are predicted by the in silico digestion of the Eg133H20 BAC sequence with the appropriate restriction enzyme (a: 4,069 bp <t>EcoRI</t> fragment; b: 4,841 bp HindIII fragment; c: 6,251 <t>BamHI</t> fragment; d: 6,138 bp HindIII fragment; e: 13,132 bp BamHI fragment), asterisks indicate supernumerary bands.
    Restriction Enzyme Bamhi, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 36 article reviews
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    85
    Promega bamh1 restriction enzyme
    PCR ( A ), Southern blot ( B ) and sequence analysis ( C ) of the selected zeocin-resistant clones. (A). Aliquots from three different clones obtained from cells that expressed wt IN (wt 1–3 ), D116A IN (D116A 1–3 ), or no IN (-IN) were subjected to total genomic DNA extraction and PCR using 5′-U3-Zeo and 3′-U5-Zeo primers (B). Total genomic DNA was digested by <t>BamH1</t> restriction enzyme and submitted to Southern blot analysis with (5′- 32 P) 5′-U3-Zeo as a probe. M: DNA size marker (bp). (C) Total genomic DNA was cut by BamH1 restriction enzyme, ligated, amplified using 5′-U3-junction and 3′-U5-junction primers and sequenced using the same primers. The name of the disrupted ORF is mentioned as well as the integration site inside the gene. The 5 bp repeats are underlined, dotted lines indicate base deletions at the integration site. ( D ) The sequences of the integrated products obtained either in an in vitro concerted integration assay [data obtained from Ref. ( 17 )] or from the in vivo yeast integration system were compared. The number of duplications and deletions in both systems are reported. 'Other duplications' indicate the number of cases that exhibited duplications comprising sizes from 3 to 6 bp.
    Bamh1 Restriction Enzyme, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Promega bamh1 restriction endonucleases
    PCR ( A ), Southern blot ( B ) and sequence analysis ( C ) of the selected zeocin-resistant clones. (A). Aliquots from three different clones obtained from cells that expressed wt IN (wt 1–3 ), D116A IN (D116A 1–3 ), or no IN (-IN) were subjected to total genomic DNA extraction and PCR using 5′-U3-Zeo and 3′-U5-Zeo primers (B). Total genomic DNA was digested by <t>BamH1</t> restriction enzyme and submitted to Southern blot analysis with (5′- 32 P) 5′-U3-Zeo as a probe. M: DNA size marker (bp). (C) Total genomic DNA was cut by BamH1 restriction enzyme, ligated, amplified using 5′-U3-junction and 3′-U5-junction primers and sequenced using the same primers. The name of the disrupted ORF is mentioned as well as the integration site inside the gene. The 5 bp repeats are underlined, dotted lines indicate base deletions at the integration site. ( D ) The sequences of the integrated products obtained either in an in vitro concerted integration assay [data obtained from Ref. ( 17 )] or from the in vivo yeast integration system were compared. The number of duplications and deletions in both systems are reported. 'Other duplications' indicate the number of cases that exhibited duplications comprising sizes from 3 to 6 bp.
    Bamh1 Restriction Endonucleases, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Promega restriction enzymes
    PCR ( A ), Southern blot ( B ) and sequence analysis ( C ) of the selected zeocin-resistant clones. (A). Aliquots from three different clones obtained from cells that expressed wt IN (wt 1–3 ), D116A IN (D116A 1–3 ), or no IN (-IN) were subjected to total genomic DNA extraction and PCR using 5′-U3-Zeo and 3′-U5-Zeo primers (B). Total genomic DNA was digested by <t>BamH1</t> restriction enzyme and submitted to Southern blot analysis with (5′- 32 P) 5′-U3-Zeo as a probe. M: DNA size marker (bp). (C) Total genomic DNA was cut by BamH1 restriction enzyme, ligated, amplified using 5′-U3-junction and 3′-U5-junction primers and sequenced using the same primers. The name of the disrupted ORF is mentioned as well as the integration site inside the gene. The 5 bp repeats are underlined, dotted lines indicate base deletions at the integration site. ( D ) The sequences of the integrated products obtained either in an in vitro concerted integration assay [data obtained from Ref. ( 17 )] or from the in vivo yeast integration system were compared. The number of duplications and deletions in both systems are reported. 'Other duplications' indicate the number of cases that exhibited duplications comprising sizes from 3 to 6 bp.
    Restriction Enzymes, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 486 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Promega restriction endonuclease bamhi
    Restriction enzyme analysis of genomic Arg827/04 <t>DNA</t> in comparison with HAdV-3p strain GB and HAdV-16p strain Ch79. <t>BamHI</t> profiles for HAdV-16p and the 3-16 intertypic recombinant Arg827/04 exhibited identical band patterns, which are illustrated in a single lane. HindIII profiles for HAdV-3p and the 3-16 intertypic recombinant Arg827/04 exhibited identical band patterns that were also illustrated in a single lane. M, 1-kbp plus 100-bp molecular markers.
    Restriction Endonuclease Bamhi, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Promega bamhi hindiii
    Restriction enzyme analysis of genomic Arg827/04 <t>DNA</t> in comparison with HAdV-3p strain GB and HAdV-16p strain Ch79. <t>BamHI</t> profiles for HAdV-16p and the 3-16 intertypic recombinant Arg827/04 exhibited identical band patterns, which are illustrated in a single lane. HindIII profiles for HAdV-3p and the 3-16 intertypic recombinant Arg827/04 exhibited identical band patterns that were also illustrated in a single lane. M, 1-kbp plus 100-bp molecular markers.
    Bamhi Hindiii, supplied by Promega, used in various techniques. Bioz Stars score: 89/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Determination of copy number by Southern blot. A: EgDEF1 gene; B: gypsy retroelement. One L2T-related genotype (left: LMC343 or LMC51) is compared to one non-L2T-related genotype (right: FC2317 or FC2318). Lowercase letters signal the hybridizing bands that are predicted by the in silico digestion of the Eg133H20 BAC sequence with the appropriate restriction enzyme (a: 4,069 bp EcoRI fragment; b: 4,841 bp HindIII fragment; c: 6,251 BamHI fragment; d: 6,138 bp HindIII fragment; e: 13,132 bp BamHI fragment), asterisks indicate supernumerary bands.

    Journal: PLoS ONE

    Article Title: DNA Methylation and Expression of the EgDEF1 Gene and Neighboring Retrotransposons in mantled Somaclonal Variants of Oil Palm

    doi: 10.1371/journal.pone.0091896

    Figure Lengend Snippet: Determination of copy number by Southern blot. A: EgDEF1 gene; B: gypsy retroelement. One L2T-related genotype (left: LMC343 or LMC51) is compared to one non-L2T-related genotype (right: FC2317 or FC2318). Lowercase letters signal the hybridizing bands that are predicted by the in silico digestion of the Eg133H20 BAC sequence with the appropriate restriction enzyme (a: 4,069 bp EcoRI fragment; b: 4,841 bp HindIII fragment; c: 6,251 BamHI fragment; d: 6,138 bp HindIII fragment; e: 13,132 bp BamHI fragment), asterisks indicate supernumerary bands.

    Article Snippet: Southern blot Seven micrograms of oil palm genomic DNA extracts were digested in parallel by either EcoRI, HindIII or BamHI restriction enzymes (Promega) according to the manufacturer's instructions.

    Techniques: Southern Blot, In Silico, BAC Assay, Sequencing

    PCR ( A ), Southern blot ( B ) and sequence analysis ( C ) of the selected zeocin-resistant clones. (A). Aliquots from three different clones obtained from cells that expressed wt IN (wt 1–3 ), D116A IN (D116A 1–3 ), or no IN (-IN) were subjected to total genomic DNA extraction and PCR using 5′-U3-Zeo and 3′-U5-Zeo primers (B). Total genomic DNA was digested by BamH1 restriction enzyme and submitted to Southern blot analysis with (5′- 32 P) 5′-U3-Zeo as a probe. M: DNA size marker (bp). (C) Total genomic DNA was cut by BamH1 restriction enzyme, ligated, amplified using 5′-U3-junction and 3′-U5-junction primers and sequenced using the same primers. The name of the disrupted ORF is mentioned as well as the integration site inside the gene. The 5 bp repeats are underlined, dotted lines indicate base deletions at the integration site. ( D ) The sequences of the integrated products obtained either in an in vitro concerted integration assay [data obtained from Ref. ( 17 )] or from the in vivo yeast integration system were compared. The number of duplications and deletions in both systems are reported. 'Other duplications' indicate the number of cases that exhibited duplications comprising sizes from 3 to 6 bp.

    Journal: Nucleic Acids Research

    Article Title: Chromosomal integration of LTR-flanked DNA in yeast expressing HIV-1 integrase: down regulation by RAD51

    doi: 10.1093/nar/gkl843

    Figure Lengend Snippet: PCR ( A ), Southern blot ( B ) and sequence analysis ( C ) of the selected zeocin-resistant clones. (A). Aliquots from three different clones obtained from cells that expressed wt IN (wt 1–3 ), D116A IN (D116A 1–3 ), or no IN (-IN) were subjected to total genomic DNA extraction and PCR using 5′-U3-Zeo and 3′-U5-Zeo primers (B). Total genomic DNA was digested by BamH1 restriction enzyme and submitted to Southern blot analysis with (5′- 32 P) 5′-U3-Zeo as a probe. M: DNA size marker (bp). (C) Total genomic DNA was cut by BamH1 restriction enzyme, ligated, amplified using 5′-U3-junction and 3′-U5-junction primers and sequenced using the same primers. The name of the disrupted ORF is mentioned as well as the integration site inside the gene. The 5 bp repeats are underlined, dotted lines indicate base deletions at the integration site. ( D ) The sequences of the integrated products obtained either in an in vitro concerted integration assay [data obtained from Ref. ( 17 )] or from the in vivo yeast integration system were compared. The number of duplications and deletions in both systems are reported. 'Other duplications' indicate the number of cases that exhibited duplications comprising sizes from 3 to 6 bp.

    Article Snippet: Southern blot analysis was performed on genomic DNA according to a previously described protocol ( ) and using BamH1 restriction enzyme (PROMEGA) and the 32 P-labeled ODN, 5′-32 P-U3-Zeo described above as probe.

    Techniques: Polymerase Chain Reaction, Southern Blot, Sequencing, Clone Assay, DNA Extraction, Marker, Amplification, In Vitro, In Vivo

    Restriction enzyme analysis of genomic Arg827/04 DNA in comparison with HAdV-3p strain GB and HAdV-16p strain Ch79. BamHI profiles for HAdV-16p and the 3-16 intertypic recombinant Arg827/04 exhibited identical band patterns, which are illustrated in a single lane. HindIII profiles for HAdV-3p and the 3-16 intertypic recombinant Arg827/04 exhibited identical band patterns that were also illustrated in a single lane. M, 1-kbp plus 100-bp molecular markers.

    Journal: Journal of Clinical Microbiology

    Article Title: Molecular Characterization of an Adenovirus 3-16 Intertypic Recombinant Isolated in Argentina from an Infant Hospitalized with Acute Respiratory Infection ▿

    doi: 10.1128/JCM.02289-09

    Figure Lengend Snippet: Restriction enzyme analysis of genomic Arg827/04 DNA in comparison with HAdV-3p strain GB and HAdV-16p strain Ch79. BamHI profiles for HAdV-16p and the 3-16 intertypic recombinant Arg827/04 exhibited identical band patterns, which are illustrated in a single lane. HindIII profiles for HAdV-3p and the 3-16 intertypic recombinant Arg827/04 exhibited identical band patterns that were also illustrated in a single lane. M, 1-kbp plus 100-bp molecular markers.

    Article Snippet: For genomic characterization and genome type identification by REA, 1 μg of viral DNA was initially digested with restriction endonuclease BamHI according to the manufacturer's recommendations (Promega, Madison, WI) and further characterized by digestion with BglII, HindIII, SmaI, and XhoI.

    Techniques: Recombinant