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  • 94
    Promega bamhi restriction enzymes
    Bamhi Restriction Enzymes, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Promega bamhi restriction enzyme
    Bamhi Restriction Enzyme, supplied by Promega, used in various techniques. Bioz Stars score: 79/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    77
    Promega bamh1 restriction enzyme
    PCR ( A ), Southern blot ( B ) and sequence analysis ( C ) of the selected zeocin-resistant clones. (A). Aliquots from three different clones obtained from cells that expressed wt IN (wt 1–3 ), D116A IN (D116A 1–3 ), or no IN (-IN) were subjected to total genomic DNA extraction and PCR using 5′-U3-Zeo and 3′-U5-Zeo primers (B). Total genomic DNA was digested by <t>BamH1</t> restriction enzyme and submitted to Southern blot analysis with (5′- 32 P) 5′-U3-Zeo as a probe. M: DNA size marker (bp). (C) Total genomic DNA was cut by BamH1 restriction enzyme, ligated, amplified using 5′-U3-junction and 3′-U5-junction primers and sequenced using the same primers. The name of the disrupted ORF is mentioned as well as the integration site inside the gene. The 5 bp repeats are underlined, dotted lines indicate base deletions at the integration site. ( D ) The sequences of the integrated products obtained either in an in vitro concerted integration assay [data obtained from Ref. ( 17 )] or from the in vivo yeast integration system were compared. The number of duplications and deletions in both systems are reported. 'Other duplications' indicate the number of cases that exhibited duplications comprising sizes from 3 to 6 bp.
    Bamh1 Restriction Enzyme, supplied by Promega, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamh1 restriction enzyme/product/Promega
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    76
    Promega bamh1 restriction endonucleases
    PCR ( A ), Southern blot ( B ) and sequence analysis ( C ) of the selected zeocin-resistant clones. (A). Aliquots from three different clones obtained from cells that expressed wt IN (wt 1–3 ), D116A IN (D116A 1–3 ), or no IN (-IN) were subjected to total genomic DNA extraction and PCR using 5′-U3-Zeo and 3′-U5-Zeo primers (B). Total genomic DNA was digested by <t>BamH1</t> restriction enzyme and submitted to Southern blot analysis with (5′- 32 P) 5′-U3-Zeo as a probe. M: DNA size marker (bp). (C) Total genomic DNA was cut by BamH1 restriction enzyme, ligated, amplified using 5′-U3-junction and 3′-U5-junction primers and sequenced using the same primers. The name of the disrupted ORF is mentioned as well as the integration site inside the gene. The 5 bp repeats are underlined, dotted lines indicate base deletions at the integration site. ( D ) The sequences of the integrated products obtained either in an in vitro concerted integration assay [data obtained from Ref. ( 17 )] or from the in vivo yeast integration system were compared. The number of duplications and deletions in both systems are reported. 'Other duplications' indicate the number of cases that exhibited duplications comprising sizes from 3 to 6 bp.
    Bamh1 Restriction Endonucleases, supplied by Promega, used in various techniques. Bioz Stars score: 76/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Promega bamhi
    PCR ( A ), Southern blot ( B ) and sequence analysis ( C ) of the selected zeocin-resistant clones. (A). Aliquots from three different clones obtained from cells that expressed wt IN (wt 1–3 ), D116A IN (D116A 1–3 ), or no IN (-IN) were subjected to total genomic DNA extraction and PCR using 5′-U3-Zeo and 3′-U5-Zeo primers (B). Total genomic DNA was digested by <t>BamH1</t> restriction enzyme and submitted to Southern blot analysis with (5′- 32 P) 5′-U3-Zeo as a probe. M: DNA size marker (bp). (C) Total genomic DNA was cut by BamH1 restriction enzyme, ligated, amplified using 5′-U3-junction and 3′-U5-junction primers and sequenced using the same primers. The name of the disrupted ORF is mentioned as well as the integration site inside the gene. The 5 bp repeats are underlined, dotted lines indicate base deletions at the integration site. ( D ) The sequences of the integrated products obtained either in an in vitro concerted integration assay [data obtained from Ref. ( 17 )] or from the in vivo yeast integration system were compared. The number of duplications and deletions in both systems are reported. 'Other duplications' indicate the number of cases that exhibited duplications comprising sizes from 3 to 6 bp.
    Bamhi, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 1956 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bamhi/product/Promega
    Average 94 stars, based on 1956 article reviews
    Price from $9.99 to $1999.99
    bamhi - by Bioz Stars, 2020-01
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    PCR ( A ), Southern blot ( B ) and sequence analysis ( C ) of the selected zeocin-resistant clones. (A). Aliquots from three different clones obtained from cells that expressed wt IN (wt 1–3 ), D116A IN (D116A 1–3 ), or no IN (-IN) were subjected to total genomic DNA extraction and PCR using 5′-U3-Zeo and 3′-U5-Zeo primers (B). Total genomic DNA was digested by BamH1 restriction enzyme and submitted to Southern blot analysis with (5′- 32 P) 5′-U3-Zeo as a probe. M: DNA size marker (bp). (C) Total genomic DNA was cut by BamH1 restriction enzyme, ligated, amplified using 5′-U3-junction and 3′-U5-junction primers and sequenced using the same primers. The name of the disrupted ORF is mentioned as well as the integration site inside the gene. The 5 bp repeats are underlined, dotted lines indicate base deletions at the integration site. ( D ) The sequences of the integrated products obtained either in an in vitro concerted integration assay [data obtained from Ref. ( 17 )] or from the in vivo yeast integration system were compared. The number of duplications and deletions in both systems are reported. 'Other duplications' indicate the number of cases that exhibited duplications comprising sizes from 3 to 6 bp.

    Journal: Nucleic Acids Research

    Article Title: Chromosomal integration of LTR-flanked DNA in yeast expressing HIV-1 integrase: down regulation by RAD51

    doi: 10.1093/nar/gkl843

    Figure Lengend Snippet: PCR ( A ), Southern blot ( B ) and sequence analysis ( C ) of the selected zeocin-resistant clones. (A). Aliquots from three different clones obtained from cells that expressed wt IN (wt 1–3 ), D116A IN (D116A 1–3 ), or no IN (-IN) were subjected to total genomic DNA extraction and PCR using 5′-U3-Zeo and 3′-U5-Zeo primers (B). Total genomic DNA was digested by BamH1 restriction enzyme and submitted to Southern blot analysis with (5′- 32 P) 5′-U3-Zeo as a probe. M: DNA size marker (bp). (C) Total genomic DNA was cut by BamH1 restriction enzyme, ligated, amplified using 5′-U3-junction and 3′-U5-junction primers and sequenced using the same primers. The name of the disrupted ORF is mentioned as well as the integration site inside the gene. The 5 bp repeats are underlined, dotted lines indicate base deletions at the integration site. ( D ) The sequences of the integrated products obtained either in an in vitro concerted integration assay [data obtained from Ref. ( 17 )] or from the in vivo yeast integration system were compared. The number of duplications and deletions in both systems are reported. 'Other duplications' indicate the number of cases that exhibited duplications comprising sizes from 3 to 6 bp.

    Article Snippet: Southern blot analysis was performed on genomic DNA according to a previously described protocol ( ) and using BamH1 restriction enzyme (PROMEGA) and the 32 P-labeled ODN, 5′-32 P-U3-Zeo described above as probe.

    Techniques: Polymerase Chain Reaction, Southern Blot, Sequencing, Clone Assay, DNA Extraction, Marker, Amplification, In Vitro, In Vivo