bamhi restriction enzymes New England Biolabs Search Results


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  • 99
    Thermo Fisher bamhi
    Bamhi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9497 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 9497 article reviews
    Price from $9.99 to $1999.99
    bamhi - by Bioz Stars, 2020-01
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    85
    New England Biolabs enzymes bamhi
    Enzymes Bamhi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 85/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzymes bamhi/product/New England Biolabs
    Average 85 stars, based on 14 article reviews
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    enzymes bamhi - by Bioz Stars, 2020-01
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    89
    New England Biolabs restriction enzymes bamhi hf
    Restriction Enzymes Bamhi Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 89/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 89 stars, based on 20 article reviews
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    99
    New England Biolabs ecori
    Ecori, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 8258 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs sali hf
    Restriction map of a FLASH plasmid harboring an extension unit Extension units are released from their plasmid vectors using a quadruple digest. In the example shown, the extension unit contains coding sequence for four TALE repeats (colored rectangles). Digestion of the plasmid with BbsI and <t>BamHI</t> releases the unit from the plasmid with appropriate overhangs for use in the FLASH assembly method. Digestion of the plasmid with the additional restriction enzymes XbaI and <t>SalI</t> prevents the released unit fragment religating back into the vector, thereby eliminating the need to gel purify the fragment.
    Sali Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 285 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sali hf/product/New England Biolabs
    Average 99 stars, based on 285 article reviews
    Price from $9.99 to $1999.99
    sali hf - by Bioz Stars, 2020-01
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    Image Search Results


    Restriction map of a FLASH plasmid harboring an extension unit Extension units are released from their plasmid vectors using a quadruple digest. In the example shown, the extension unit contains coding sequence for four TALE repeats (colored rectangles). Digestion of the plasmid with BbsI and BamHI releases the unit from the plasmid with appropriate overhangs for use in the FLASH assembly method. Digestion of the plasmid with the additional restriction enzymes XbaI and SalI prevents the released unit fragment religating back into the vector, thereby eliminating the need to gel purify the fragment.

    Journal: Current protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]

    Article Title: Engineering Customized TALE Nucleases (TALENs) and TALE Transcription Factors by Fast Ligation-based Automatable Solid-phase High-throughput (FLASH) Assembly

    doi: 10.1002/0471142727.mb1216s103

    Figure Lengend Snippet: Restriction map of a FLASH plasmid harboring an extension unit Extension units are released from their plasmid vectors using a quadruple digest. In the example shown, the extension unit contains coding sequence for four TALE repeats (colored rectangles). Digestion of the plasmid with BbsI and BamHI releases the unit from the plasmid with appropriate overhangs for use in the FLASH assembly method. Digestion of the plasmid with the additional restriction enzymes XbaI and SalI prevents the released unit fragment religating back into the vector, thereby eliminating the need to gel purify the fragment.

    Article Snippet: ) 100X Bovine Serum Albumin (BSA) (10 mg/ml) (NEB cat. no. B9001S) Restriction enzymes (NEB): BamHI-HF (cat. no. R3136L) XbaI (cat. no. R0145L) BbsI (cat. no. R0539L) SalI-HF (cat. no. R3138L).

    Techniques: Plasmid Preparation, Sequencing