bamhi restriction endonuclease Search Results


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  • 99
    New England Biolabs dna restriction enzymes
    Map of PCR primer binding sites and restriction enzyme cut sizes for the barcode fragment of COI. <t>BamHI</t> restriction sites are only found in haplotype group A alleles while AflIII and <t>BseYI</t> restriction sites are only found in haplotype group B alleles.
    Dna Restriction Enzymes, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 458 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher bamhi restriction endonuclease
    Degradation of linearized mtDNA in control and mutant mitoEagI-expressing HEK 293 cells. a Southern blot showing the degradation of mtDNA within the first 18 h of induced expression of mitoEagI (E) in control, MGME1 p.I9Qfs*32 knockout (‘MGME1 ko’), and exonuclease-deficient POLG (‘POLG p.D274A’) cells. <t>BamHI</t> endonuclease-linearized <t>DNA</t> (B) was labeled with a mitochondrial probe represented by an asterisk as well as a probe specific for nuclear 18S ribosomal DNA (‘18S’). Note that persistent bands with one end in the vicinity of oriL in MGME1 ko and mutated POLG cells are already present before induction (time point ‘0’, lowest arrowhead). These linear mtDNA species are due to leaky mitoEagI expression and their presence is not related to the induced massive double-strand breaks. b Quantification of full-length mtDNA confirms the efficient cleavage of mtDNA by mitoEagI and ( c ) the persistence of mitoEagI-linearized mtDNA in MGME1 ko and POLG p.D274A cells. Band intensities were first normalized to 18S ribosomal DNA intensities then to intensities of the full-length mtDNA in each cell line before induction. Error bars represent standard errors of the mean (SEM) in three independent experiments (including both available MGME1 knockout clones). Significance was calculated by applying one-way ANOVA test. * P
    Bamhi Restriction Endonuclease, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Roche restriction endonucleases bamhi
    Degradation of linearized mtDNA in control and mutant mitoEagI-expressing HEK 293 cells. a Southern blot showing the degradation of mtDNA within the first 18 h of induced expression of mitoEagI (E) in control, MGME1 p.I9Qfs*32 knockout (‘MGME1 ko’), and exonuclease-deficient POLG (‘POLG p.D274A’) cells. <t>BamHI</t> endonuclease-linearized <t>DNA</t> (B) was labeled with a mitochondrial probe represented by an asterisk as well as a probe specific for nuclear 18S ribosomal DNA (‘18S’). Note that persistent bands with one end in the vicinity of oriL in MGME1 ko and mutated POLG cells are already present before induction (time point ‘0’, lowest arrowhead). These linear mtDNA species are due to leaky mitoEagI expression and their presence is not related to the induced massive double-strand breaks. b Quantification of full-length mtDNA confirms the efficient cleavage of mtDNA by mitoEagI and ( c ) the persistence of mitoEagI-linearized mtDNA in MGME1 ko and POLG p.D274A cells. Band intensities were first normalized to 18S ribosomal DNA intensities then to intensities of the full-length mtDNA in each cell line before induction. Error bars represent standard errors of the mean (SEM) in three independent experiments (including both available MGME1 knockout clones). Significance was calculated by applying one-way ANOVA test. * P
    Restriction Endonucleases Bamhi, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    TaKaRa bamhi restriction endonuclease
    Degradation of linearized mtDNA in control and mutant mitoEagI-expressing HEK 293 cells. a Southern blot showing the degradation of mtDNA within the first 18 h of induced expression of mitoEagI (E) in control, MGME1 p.I9Qfs*32 knockout (‘MGME1 ko’), and exonuclease-deficient POLG (‘POLG p.D274A’) cells. <t>BamHI</t> endonuclease-linearized <t>DNA</t> (B) was labeled with a mitochondrial probe represented by an asterisk as well as a probe specific for nuclear 18S ribosomal DNA (‘18S’). Note that persistent bands with one end in the vicinity of oriL in MGME1 ko and mutated POLG cells are already present before induction (time point ‘0’, lowest arrowhead). These linear mtDNA species are due to leaky mitoEagI expression and their presence is not related to the induced massive double-strand breaks. b Quantification of full-length mtDNA confirms the efficient cleavage of mtDNA by mitoEagI and ( c ) the persistence of mitoEagI-linearized mtDNA in MGME1 ko and POLG p.D274A cells. Band intensities were first normalized to 18S ribosomal DNA intensities then to intensities of the full-length mtDNA in each cell line before induction. Error bars represent standard errors of the mean (SEM) in three independent experiments (including both available MGME1 knockout clones). Significance was calculated by applying one-way ANOVA test. * P
    Bamhi Restriction Endonuclease, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    SibEnzyme bamhi restriction endonucleases
    Number of lesions per 10 kb <t>mtDNA</t> induced by AhlI and <t>BamHI</t> restriction endonucleases. Fragment 1 had an AhlI recognition site; fragment 2 had a BamHI recognition site; fragment 3 had recognition site for both restriction endonucleases; fragment 4 lacked recognition sites for either AhlI or BamHI.
    Bamhi Restriction Endonucleases, supplied by SibEnzyme, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher restriction endonucleases bamhi
    PCR amplification and restriction enzyme digestion of light chain and H CC coding sequences. Agarose gel electrophoresis of PCR products of light chain and H CC fragments confirms their 1371 and 621 bp size, respectively; A) Double digestion of pET28b(+) light chain and H CC with <t>BamHI</t> and <t>HindIII</t> endonucleases indicates insertion of these two gene segments into the expression vector; B) SM: DNA size marker, bp : base pair
    Restriction Endonucleases Bamhi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 180 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs bamhi restriction endonuclease enzyme
    Twinkle displaces <t>BamHI-E111A</t> bound to forked duplex DNA substrate and unwinds the forked duplex. A and B, indicated concentrations of Twinkle (hexamer) were incubated at 37 °C for 30 min with a BamHI forked duplex substrate (substrate 27) (0.5
    Bamhi Restriction Endonuclease Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Promega restriction endonuclease bamhi
    Restriction enzyme analysis of genomic Arg827/04 <t>DNA</t> in comparison with HAdV-3p strain GB and HAdV-16p strain Ch79. <t>BamHI</t> profiles for HAdV-16p and the 3-16 intertypic recombinant Arg827/04 exhibited identical band patterns, which are illustrated in a single lane. HindIII profiles for HAdV-3p and the 3-16 intertypic recombinant Arg827/04 exhibited identical band patterns that were also illustrated in a single lane. M, 1-kbp plus 100-bp molecular markers.
    Restriction Endonuclease Bamhi, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    New England Biolabs bamhi hf restriction endonuclease
    Restriction enzyme analysis of genomic Arg827/04 <t>DNA</t> in comparison with HAdV-3p strain GB and HAdV-16p strain Ch79. <t>BamHI</t> profiles for HAdV-16p and the 3-16 intertypic recombinant Arg827/04 exhibited identical band patterns, which are illustrated in a single lane. HindIII profiles for HAdV-3p and the 3-16 intertypic recombinant Arg827/04 exhibited identical band patterns that were also illustrated in a single lane. M, 1-kbp plus 100-bp molecular markers.
    Bamhi Hf Restriction Endonuclease, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs dna restriction endonucleases
    Partial schematic maps of <t>DNA/protein</t> sequence in the beginning (a, b) or the end (c, d) regions of recombinant CPP-dmCocE fusion proteins on the corresponding expression vectors. Except <t>BamHI</t> sites, all other restriction sites (NdeI, XhoI) are from the
    Dna Restriction Endonucleases, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 154 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs restriction endonuclease digestion
    Partial schematic maps of <t>DNA/protein</t> sequence in the beginning (a, b) or the end (c, d) regions of recombinant CPP-dmCocE fusion proteins on the corresponding expression vectors. Except <t>BamHI</t> sites, all other restriction sites (NdeI, XhoI) are from the
    Restriction Endonuclease Digestion, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 405 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Thermo Fisher bamhi enzymes
    Electrophoresis of digested pBSC-ptr using enzyme/ Lane 1: Digested pBSC-ptr by <t>KpnI</t> and <t>BamHI/Lane</t> 2: 100-bp DNA ladder marker
    Bamhi Enzymes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Map of PCR primer binding sites and restriction enzyme cut sizes for the barcode fragment of COI. BamHI restriction sites are only found in haplotype group A alleles while AflIII and BseYI restriction sites are only found in haplotype group B alleles.

    Journal: Systematic entomology

    Article Title: A tale of two haplotype groups: Evaluating the New World Junonia ring species hypothesis using the distribution of divergent COI haplotypes

    doi: 10.1111/syen.12120

    Figure Lengend Snippet: Map of PCR primer binding sites and restriction enzyme cut sizes for the barcode fragment of COI. BamHI restriction sites are only found in haplotype group A alleles while AflIII and BseYI restriction sites are only found in haplotype group B alleles.

    Article Snippet: PCR products of the correct size were evaluated by a triple digest using BseYI, AflIII, and BamHI restriction endonucleases (New England Biolabs, Ipswich, MA, USA) ( ).

    Techniques: Polymerase Chain Reaction, Binding Assay

    Digestion and Southern analyses of the P-SSP7 genome. (A) Schematic genome map showing the positions of the restriction enzyme cleavage sites (red) and the expected fragment sizes after digestion with BamHI alone (top) and both BamHI and PmeI (bottom) based on the revised genome arrangement shown in Fig. 1C. (B) Restriction digestion of the P-SSP7 genome extracted from phage particles (lanes 3 and 4) and the genome cloned into a fosmid (lanes 5 and 6), with BamHI alone (lanes 3 and 5) or with BamHI and PmeI (lanes 4 and 6), separated by pulse field gel electrophoresis. Note that the only difference for digestion of the cloned genome is the presence of an additional fragment corresponding to the size of the fosmid vector. Fragments corresponding to the expected sizes shown in (A) are marked with the appropriate letter designations (a to f). Fragment size markers (M): 1 kb DNA ladder (lane 1) and Lambda DNA cut with HindIII (lane 2), are shown. (C) Southern analyses of the restriction digested DNA in (B) using 4 probes (denoted above the lanes) show that the repeat region appears twice on the genome on the same fragments as the first and last ORFs. The positions of the gene probes on the genome are shown as light blue boxes and the repeat region probe as green boxes in the top panel of (A). Lane numbering and fragment designations are the same as in (B).

    Journal: PLoS ONE

    Article Title: The P-SSP7 Cyanophage Has a Linear Genome with Direct Terminal Repeats

    doi: 10.1371/journal.pone.0036710

    Figure Lengend Snippet: Digestion and Southern analyses of the P-SSP7 genome. (A) Schematic genome map showing the positions of the restriction enzyme cleavage sites (red) and the expected fragment sizes after digestion with BamHI alone (top) and both BamHI and PmeI (bottom) based on the revised genome arrangement shown in Fig. 1C. (B) Restriction digestion of the P-SSP7 genome extracted from phage particles (lanes 3 and 4) and the genome cloned into a fosmid (lanes 5 and 6), with BamHI alone (lanes 3 and 5) or with BamHI and PmeI (lanes 4 and 6), separated by pulse field gel electrophoresis. Note that the only difference for digestion of the cloned genome is the presence of an additional fragment corresponding to the size of the fosmid vector. Fragments corresponding to the expected sizes shown in (A) are marked with the appropriate letter designations (a to f). Fragment size markers (M): 1 kb DNA ladder (lane 1) and Lambda DNA cut with HindIII (lane 2), are shown. (C) Southern analyses of the restriction digested DNA in (B) using 4 probes (denoted above the lanes) show that the repeat region appears twice on the genome on the same fragments as the first and last ORFs. The positions of the gene probes on the genome are shown as light blue boxes and the repeat region probe as green boxes in the top panel of (A). Lane numbering and fragment designations are the same as in (B).

    Article Snippet: The DNA (0.5 µg per reaction) was digested with BamHI and with a combination of BamHI and PmeI (New England Biolabs).

    Techniques: Clone Assay, Nucleic Acid Electrophoresis, Plasmid Preparation, Lambda DNA Preparation

    Schematic illustration of the arrangement of the P-SSP7 genome. (A) Sequencing of the ends of the P-SSP7 genome extracted directly from phage particles. Arrows, and numbers under the arrows, indicate the sequences acquired: Blue from the entire genome and green from end fragments produced by digestion of the genome with the BamHI and PmeI restriction enzymes. The positions of the primers used for sequencing are shown in black type at the beginning of the arrows. Genome numbering for the primers and sequences is that for the originally published sequence [5] . The purple line denotes the 728 bp region found to be upstream of ORF1 in this study, but positioned downstream of ORF54 in the originally published sequence. The repeat regions are shown in red at both ends of the genome. (B) Diagram showing the arrangement of the P-SSP7 genome as originally published (GenBank accession numbers: AY939843.1, [5] and GU071093 [16] . (C) Diagram of the revised genome arrangement based on the results from this study (updated GeneBank submission, accession number: AY939843.2).

    Journal: PLoS ONE

    Article Title: The P-SSP7 Cyanophage Has a Linear Genome with Direct Terminal Repeats

    doi: 10.1371/journal.pone.0036710

    Figure Lengend Snippet: Schematic illustration of the arrangement of the P-SSP7 genome. (A) Sequencing of the ends of the P-SSP7 genome extracted directly from phage particles. Arrows, and numbers under the arrows, indicate the sequences acquired: Blue from the entire genome and green from end fragments produced by digestion of the genome with the BamHI and PmeI restriction enzymes. The positions of the primers used for sequencing are shown in black type at the beginning of the arrows. Genome numbering for the primers and sequences is that for the originally published sequence [5] . The purple line denotes the 728 bp region found to be upstream of ORF1 in this study, but positioned downstream of ORF54 in the originally published sequence. The repeat regions are shown in red at both ends of the genome. (B) Diagram showing the arrangement of the P-SSP7 genome as originally published (GenBank accession numbers: AY939843.1, [5] and GU071093 [16] . (C) Diagram of the revised genome arrangement based on the results from this study (updated GeneBank submission, accession number: AY939843.2).

    Article Snippet: The DNA (0.5 µg per reaction) was digested with BamHI and with a combination of BamHI and PmeI (New England Biolabs).

    Techniques: Sequencing, Produced

    Degradation of linearized mtDNA in control and mutant mitoEagI-expressing HEK 293 cells. a Southern blot showing the degradation of mtDNA within the first 18 h of induced expression of mitoEagI (E) in control, MGME1 p.I9Qfs*32 knockout (‘MGME1 ko’), and exonuclease-deficient POLG (‘POLG p.D274A’) cells. BamHI endonuclease-linearized DNA (B) was labeled with a mitochondrial probe represented by an asterisk as well as a probe specific for nuclear 18S ribosomal DNA (‘18S’). Note that persistent bands with one end in the vicinity of oriL in MGME1 ko and mutated POLG cells are already present before induction (time point ‘0’, lowest arrowhead). These linear mtDNA species are due to leaky mitoEagI expression and their presence is not related to the induced massive double-strand breaks. b Quantification of full-length mtDNA confirms the efficient cleavage of mtDNA by mitoEagI and ( c ) the persistence of mitoEagI-linearized mtDNA in MGME1 ko and POLG p.D274A cells. Band intensities were first normalized to 18S ribosomal DNA intensities then to intensities of the full-length mtDNA in each cell line before induction. Error bars represent standard errors of the mean (SEM) in three independent experiments (including both available MGME1 knockout clones). Significance was calculated by applying one-way ANOVA test. * P

    Journal: Nature Communications

    Article Title: Linear mitochondrial DNA is rapidly degraded by components of the replication machinery

    doi: 10.1038/s41467-018-04131-w

    Figure Lengend Snippet: Degradation of linearized mtDNA in control and mutant mitoEagI-expressing HEK 293 cells. a Southern blot showing the degradation of mtDNA within the first 18 h of induced expression of mitoEagI (E) in control, MGME1 p.I9Qfs*32 knockout (‘MGME1 ko’), and exonuclease-deficient POLG (‘POLG p.D274A’) cells. BamHI endonuclease-linearized DNA (B) was labeled with a mitochondrial probe represented by an asterisk as well as a probe specific for nuclear 18S ribosomal DNA (‘18S’). Note that persistent bands with one end in the vicinity of oriL in MGME1 ko and mutated POLG cells are already present before induction (time point ‘0’, lowest arrowhead). These linear mtDNA species are due to leaky mitoEagI expression and their presence is not related to the induced massive double-strand breaks. b Quantification of full-length mtDNA confirms the efficient cleavage of mtDNA by mitoEagI and ( c ) the persistence of mitoEagI-linearized mtDNA in MGME1 ko and POLG p.D274A cells. Band intensities were first normalized to 18S ribosomal DNA intensities then to intensities of the full-length mtDNA in each cell line before induction. Error bars represent standard errors of the mean (SEM) in three independent experiments (including both available MGME1 knockout clones). Significance was calculated by applying one-way ANOVA test. * P

    Article Snippet: One microgram of total DNA was digested with BamHI restriction endonuclease (Fermentas).

    Techniques: Mutagenesis, Expressing, Southern Blot, Knock-Out, Labeling

    Degradation of the 2.1-kb mtDNA fragment in control and siRNA-treated mitoPstI-expressing HEK 293 cells. a Southern blot showing the appearance and degradation of the 2.1-kb mtDNA fragment within the first 4 h of induced mitoPstI (P) expression in control cells and in MGME1 , POLG , or TWNK siRNA-treated HEK 293 cells. BamHI endonuclease-linearized DNA (B) was labeled with a mitochondrial probe represented by an asterisk as well as a probe specific for nuclear 18S ribosomal DNA (‘18S’). b Quantification of full-length mtDNA confirms the efficient cleavage of mtDNA by mitoPstI and c the persistence of the 2.1-kb mtDNA fragment in MGME1 , POLG , and TWNK knockdown cells. Band intensities were first normalized to 18S ribosomal DNA intensities then to intensities of the full-length mtDNA in each cell line at the starting time point and, in panel ( c ), additionally to the highest 2.1-kb fragment value on each blot. Error bars represent standard errors of the mean (SEM) in three independent experiments. Significance was calculated by applying one-way ANOVA test. * P

    Journal: Nature Communications

    Article Title: Linear mitochondrial DNA is rapidly degraded by components of the replication machinery

    doi: 10.1038/s41467-018-04131-w

    Figure Lengend Snippet: Degradation of the 2.1-kb mtDNA fragment in control and siRNA-treated mitoPstI-expressing HEK 293 cells. a Southern blot showing the appearance and degradation of the 2.1-kb mtDNA fragment within the first 4 h of induced mitoPstI (P) expression in control cells and in MGME1 , POLG , or TWNK siRNA-treated HEK 293 cells. BamHI endonuclease-linearized DNA (B) was labeled with a mitochondrial probe represented by an asterisk as well as a probe specific for nuclear 18S ribosomal DNA (‘18S’). b Quantification of full-length mtDNA confirms the efficient cleavage of mtDNA by mitoPstI and c the persistence of the 2.1-kb mtDNA fragment in MGME1 , POLG , and TWNK knockdown cells. Band intensities were first normalized to 18S ribosomal DNA intensities then to intensities of the full-length mtDNA in each cell line at the starting time point and, in panel ( c ), additionally to the highest 2.1-kb fragment value on each blot. Error bars represent standard errors of the mean (SEM) in three independent experiments. Significance was calculated by applying one-way ANOVA test. * P

    Article Snippet: One microgram of total DNA was digested with BamHI restriction endonuclease (Fermentas).

    Techniques: Expressing, Southern Blot, Labeling

    Number of lesions per 10 kb mtDNA induced by AhlI and BamHI restriction endonucleases. Fragment 1 had an AhlI recognition site; fragment 2 had a BamHI recognition site; fragment 3 had recognition site for both restriction endonucleases; fragment 4 lacked recognition sites for either AhlI or BamHI.

    Journal: Toxicology

    Article Title: Simplified qPCR method for detecting excessive mtDNA damage induced by exogenous factors

    doi: 10.1016/j.tox.2017.03.010

    Figure Lengend Snippet: Number of lesions per 10 kb mtDNA induced by AhlI and BamHI restriction endonucleases. Fragment 1 had an AhlI recognition site; fragment 2 had a BamHI recognition site; fragment 3 had recognition site for both restriction endonucleases; fragment 4 lacked recognition sites for either AhlI or BamHI.

    Article Snippet: mtDNA was treated with AhlI and BamHI restriction endonucleases (SibEnzyme, Russia) separately, and fragments 1–4 were then amplified by qPCR.

    Techniques:

    PCR amplification and restriction enzyme digestion of light chain and H CC coding sequences. Agarose gel electrophoresis of PCR products of light chain and H CC fragments confirms their 1371 and 621 bp size, respectively; A) Double digestion of pET28b(+) light chain and H CC with BamHI and HindIII endonucleases indicates insertion of these two gene segments into the expression vector; B) SM: DNA size marker, bp : base pair

    Journal: Avicenna Journal of Medical Biotechnology

    Article Title: Production and Characterization of Recombinant Light Chain and Carboxyterminal Heavy Chain Fragments of Tetanus Toxin

    doi:

    Figure Lengend Snippet: PCR amplification and restriction enzyme digestion of light chain and H CC coding sequences. Agarose gel electrophoresis of PCR products of light chain and H CC fragments confirms their 1371 and 621 bp size, respectively; A) Double digestion of pET28b(+) light chain and H CC with BamHI and HindIII endonucleases indicates insertion of these two gene segments into the expression vector; B) SM: DNA size marker, bp : base pair

    Article Snippet: After confirmation of the selected clones by sequencing, inserts were digested with restriction endonucleases BamHI and HindIII (Fermentas, Moscow, Russia) and ligated in pET28b(+) expression vector (Merck Millipore, Darmstadt, Germany). pET28b(+) light chain or HCC constructs were transformed into (E. coli ) BL21 (DE3) expression host.

    Techniques: Polymerase Chain Reaction, Amplification, Agarose Gel Electrophoresis, Expressing, Plasmid Preparation, Marker

    Twinkle displaces BamHI-E111A bound to forked duplex DNA substrate and unwinds the forked duplex. A and B, indicated concentrations of Twinkle (hexamer) were incubated at 37 °C for 30 min with a BamHI forked duplex substrate (substrate 27) (0.5

    Journal: The Journal of Biological Chemistry

    Article Title: Biochemical Characterization of the Human Mitochondrial Replicative Twinkle Helicase

    doi: 10.1074/jbc.M115.712026

    Figure Lengend Snippet: Twinkle displaces BamHI-E111A bound to forked duplex DNA substrate and unwinds the forked duplex. A and B, indicated concentrations of Twinkle (hexamer) were incubated at 37 °C for 30 min with a BamHI forked duplex substrate (substrate 27) (0.5

    Article Snippet: The catalytically inactive BamHI-E111A restriction endonuclease, used in a previous study , was provided by New England Biolabs (Ipswich, MA).

    Techniques: Incubation

    Amino acid substitutions that inactivate DNA binding by RPA negatively affect its ability to stimulate FANCJ disruption of BamHI-E111A protein-DNA substrate interaction. A ). B , reaction mixtures containing BamHI-E111A-bound forked duplex DNA substrate, FANCJ, and either wild-type RPA ( RPA ) or mutant Aro1-RPA were incubated and analyzed as described under “Experimental Procedures.” Representative gel image showing proteinase K-digested products from at least three independent experiments is shown. Star denotes 5′- 32 P end label.

    Journal: The Journal of Biological Chemistry

    Article Title: Novel Function of the Fanconi Anemia Group J or RECQ1 Helicase to Disrupt Protein-DNA Complexes in a Replication Protein A-stimulated Manner *

    doi: 10.1074/jbc.M113.542456

    Figure Lengend Snippet: Amino acid substitutions that inactivate DNA binding by RPA negatively affect its ability to stimulate FANCJ disruption of BamHI-E111A protein-DNA substrate interaction. A ). B , reaction mixtures containing BamHI-E111A-bound forked duplex DNA substrate, FANCJ, and either wild-type RPA ( RPA ) or mutant Aro1-RPA were incubated and analyzed as described under “Experimental Procedures.” Representative gel image showing proteinase K-digested products from at least three independent experiments is shown. Star denotes 5′- 32 P end label.

    Article Snippet: The 26-bp forked duplex DNA substrate containing the BamHI restriction endonuclease recognition sequence was preincubated with BamHI-E111A (38 n m ) for 15 min at 30 °C followed by addition of RPA (10 n m ) and further incubation at 30 °C for 15 min. BamHI-WT (0.25 units; New England Biolabs) was added to reaction mixtures and incubated for 15 min at 30 °C, followed by addition of proteinase K (500 μg/ml) with Stop buffer and further incubation at 37 °C for 10 min. DNA products were resolved by electrophoresis on nondenaturing 12% polyacrylamide gels.

    Techniques: Binding Assay, Recombinase Polymerase Amplification, Mutagenesis, Incubation

    Restriction enzyme analysis of genomic Arg827/04 DNA in comparison with HAdV-3p strain GB and HAdV-16p strain Ch79. BamHI profiles for HAdV-16p and the 3-16 intertypic recombinant Arg827/04 exhibited identical band patterns, which are illustrated in a single lane. HindIII profiles for HAdV-3p and the 3-16 intertypic recombinant Arg827/04 exhibited identical band patterns that were also illustrated in a single lane. M, 1-kbp plus 100-bp molecular markers.

    Journal: Journal of Clinical Microbiology

    Article Title: Molecular Characterization of an Adenovirus 3-16 Intertypic Recombinant Isolated in Argentina from an Infant Hospitalized with Acute Respiratory Infection ▿

    doi: 10.1128/JCM.02289-09

    Figure Lengend Snippet: Restriction enzyme analysis of genomic Arg827/04 DNA in comparison with HAdV-3p strain GB and HAdV-16p strain Ch79. BamHI profiles for HAdV-16p and the 3-16 intertypic recombinant Arg827/04 exhibited identical band patterns, which are illustrated in a single lane. HindIII profiles for HAdV-3p and the 3-16 intertypic recombinant Arg827/04 exhibited identical band patterns that were also illustrated in a single lane. M, 1-kbp plus 100-bp molecular markers.

    Article Snippet: For genomic characterization and genome type identification by REA, 1 μg of viral DNA was initially digested with restriction endonuclease BamHI according to the manufacturer's recommendations (Promega, Madison, WI) and further characterized by digestion with BglII, HindIII, SmaI, and XhoI.

    Techniques: Recombinant

    Partial schematic maps of DNA/protein sequence in the beginning (a, b) or the end (c, d) regions of recombinant CPP-dmCocE fusion proteins on the corresponding expression vectors. Except BamHI sites, all other restriction sites (NdeI, XhoI) are from the

    Journal: Molecular Pharmaceutics

    Article Title: Cell Permeable Cocaine Esterases Constructed by Chemical Conjugation and Genetic Recombination

    doi: 10.1021/mp200623w

    Figure Lengend Snippet: Partial schematic maps of DNA/protein sequence in the beginning (a, b) or the end (c, d) regions of recombinant CPP-dmCocE fusion proteins on the corresponding expression vectors. Except BamHI sites, all other restriction sites (NdeI, XhoI) are from the

    Article Snippet: The Phusion® site-directed mutagenesis kit and DNA restriction endonucleases (NdeI, NcoI, KpnI, BamHI) were purchased from New England Biolabs (Ipswich, MA).

    Techniques: Sequencing, Recombinant, Conditioned Place Preference, Expressing

    Electrophoresis of digested pBSC-ptr using enzyme/ Lane 1: Digested pBSC-ptr by KpnI and BamHI/Lane 2: 100-bp DNA ladder marker

    Journal: Iranian Journal of Parasitology

    Article Title: Gene Regulation of Pteridine Reductase 1 in Leishmania Promastigotes and Amastigotes Using a Full-Length Antisense Construct

    doi:

    Figure Lengend Snippet: Electrophoresis of digested pBSC-ptr using enzyme/ Lane 1: Digested pBSC-ptr by KpnI and BamHI/Lane 2: 100-bp DNA ladder marker

    Article Snippet: Construction of the antisense ptr1 gene Recombinant pBluescript containing L. major ptr1 gene (accession no. EF113119) was digested with KpnI and BamHI enzymes and purified using a Fermentas DNA purification kit (Cat. No. k0513) and then subcloned into pcDNA3 digested with KpnI and BamHI.

    Techniques: Electrophoresis, Marker

    Identification of recombinant plasmid pcDNA-rPTR using enzyme digestion. Lane 1:100-bp DNA ladder marker. Lane 2: pcDNA-rPTR digested with KpnI and BamHI

    Journal: Iranian Journal of Parasitology

    Article Title: Gene Regulation of Pteridine Reductase 1 in Leishmania Promastigotes and Amastigotes Using a Full-Length Antisense Construct

    doi:

    Figure Lengend Snippet: Identification of recombinant plasmid pcDNA-rPTR using enzyme digestion. Lane 1:100-bp DNA ladder marker. Lane 2: pcDNA-rPTR digested with KpnI and BamHI

    Article Snippet: Construction of the antisense ptr1 gene Recombinant pBluescript containing L. major ptr1 gene (accession no. EF113119) was digested with KpnI and BamHI enzymes and purified using a Fermentas DNA purification kit (Cat. No. k0513) and then subcloned into pcDNA3 digested with KpnI and BamHI.

    Techniques: Recombinant, Plasmid Preparation, Marker