bacterial alkaline phosphatase bap Search Results


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  • 86
    Thermo Fisher bacterial alkaline phosphatase bap
    Bacterial Alkaline Phosphatase Bap, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bacterial alkaline phosphatase bap/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bacterial alkaline phosphatase bap - by Bioz Stars, 2023-09
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    86
    Millipore bacterial alkaline phosphatase bap
    Affinity purification of brain lysate on LRP1b ectodomains bound <t>to</t> <t>anti-FLAG</t> agarose. FLAG-tagged ectodomains containing the ligand-binding region II, III, and IV of LRP1b were purified from the supernatant of stably transfected 293S cells using anti-FLAG agarose. The immobilized ectodomains and FLAG-tagged <t>BAP</t> as a control were incubated with the same amount of brain lysate overnight and then washed extensively. Bound proteins were eluted with 3xFLAG peptide and separated by SDS-PAGE (4 to 15% acrylamide). The nonbinding fraction (flowthrough) was diluted 1:200 and loaded as a control. Protein bands were visualized with Coomassie (ectodomains II and IV) or silver staining (ectodomain III). Bands were cut out and analyzed by reversed-phase nano-HPLC-ion-trap mass spectroscopy. Proteins identified in each band by mass spectroscopy are indicated. Ecto, ectodomain; LamRP, laminin receptor precursor.
    Bacterial Alkaline Phosphatase Bap, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bacterial alkaline phosphatase bap/product/Millipore
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bacterial alkaline phosphatase bap - by Bioz Stars, 2023-09
    86/100 stars
      Buy from Supplier

    86
    FUJIFILM bacterial alkaline phosphatase bap
    Affinity purification of brain lysate on LRP1b ectodomains bound <t>to</t> <t>anti-FLAG</t> agarose. FLAG-tagged ectodomains containing the ligand-binding region II, III, and IV of LRP1b were purified from the supernatant of stably transfected 293S cells using anti-FLAG agarose. The immobilized ectodomains and FLAG-tagged <t>BAP</t> as a control were incubated with the same amount of brain lysate overnight and then washed extensively. Bound proteins were eluted with 3xFLAG peptide and separated by SDS-PAGE (4 to 15% acrylamide). The nonbinding fraction (flowthrough) was diluted 1:200 and loaded as a control. Protein bands were visualized with Coomassie (ectodomains II and IV) or silver staining (ectodomain III). Bands were cut out and analyzed by reversed-phase nano-HPLC-ion-trap mass spectroscopy. Proteins identified in each band by mass spectroscopy are indicated. Ecto, ectodomain; LamRP, laminin receptor precursor.
    Bacterial Alkaline Phosphatase Bap, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bacterial alkaline phosphatase bap/product/FUJIFILM
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bacterial alkaline phosphatase bap - by Bioz Stars, 2023-09
    86/100 stars
      Buy from Supplier

    86
    TaKaRa bacterial alkaline phosphatase bap
    Affinity purification of brain lysate on LRP1b ectodomains bound <t>to</t> <t>anti-FLAG</t> agarose. FLAG-tagged ectodomains containing the ligand-binding region II, III, and IV of LRP1b were purified from the supernatant of stably transfected 293S cells using anti-FLAG agarose. The immobilized ectodomains and FLAG-tagged <t>BAP</t> as a control were incubated with the same amount of brain lysate overnight and then washed extensively. Bound proteins were eluted with 3xFLAG peptide and separated by SDS-PAGE (4 to 15% acrylamide). The nonbinding fraction (flowthrough) was diluted 1:200 and loaded as a control. Protein bands were visualized with Coomassie (ectodomains II and IV) or silver staining (ectodomain III). Bands were cut out and analyzed by reversed-phase nano-HPLC-ion-trap mass spectroscopy. Proteins identified in each band by mass spectroscopy are indicated. Ecto, ectodomain; LamRP, laminin receptor precursor.
    Bacterial Alkaline Phosphatase Bap, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bacterial alkaline phosphatase bap/product/TaKaRa
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bacterial alkaline phosphatase bap - by Bioz Stars, 2023-09
    86/100 stars
      Buy from Supplier

    Image Search Results


    Affinity purification of brain lysate on LRP1b ectodomains bound to anti-FLAG agarose. FLAG-tagged ectodomains containing the ligand-binding region II, III, and IV of LRP1b were purified from the supernatant of stably transfected 293S cells using anti-FLAG agarose. The immobilized ectodomains and FLAG-tagged BAP as a control were incubated with the same amount of brain lysate overnight and then washed extensively. Bound proteins were eluted with 3xFLAG peptide and separated by SDS-PAGE (4 to 15% acrylamide). The nonbinding fraction (flowthrough) was diluted 1:200 and loaded as a control. Protein bands were visualized with Coomassie (ectodomains II and IV) or silver staining (ectodomain III). Bands were cut out and analyzed by reversed-phase nano-HPLC-ion-trap mass spectroscopy. Proteins identified in each band by mass spectroscopy are indicated. Ecto, ectodomain; LamRP, laminin receptor precursor.

    Journal:

    Article Title: Normal Development and Fertility of Knockout Mice Lacking the Tumor Suppressor Gene LRP1b Suggest Functional Compensation by LRP1

    doi: 10.1128/MCB.24.9.3782-3793.2004

    Figure Lengend Snippet: Affinity purification of brain lysate on LRP1b ectodomains bound to anti-FLAG agarose. FLAG-tagged ectodomains containing the ligand-binding region II, III, and IV of LRP1b were purified from the supernatant of stably transfected 293S cells using anti-FLAG agarose. The immobilized ectodomains and FLAG-tagged BAP as a control were incubated with the same amount of brain lysate overnight and then washed extensively. Bound proteins were eluted with 3xFLAG peptide and separated by SDS-PAGE (4 to 15% acrylamide). The nonbinding fraction (flowthrough) was diluted 1:200 and loaded as a control. Protein bands were visualized with Coomassie (ectodomains II and IV) or silver staining (ectodomain III). Bands were cut out and analyzed by reversed-phase nano-HPLC-ion-trap mass spectroscopy. Proteins identified in each band by mass spectroscopy are indicated. Ecto, ectodomain; LamRP, laminin receptor precursor.

    Article Snippet: As a control, bacterial alkaline phosphatase (BAP) containing three N-terminal FLAG epitopes (Sigma) was bound to M2 anti-FLAG agarose and incubated the same way with brain tissue.

    Techniques: Affinity Purification, Ligand Binding Assay, Purification, Stable Transfection, Transfection, Incubation, SDS Page, Silver Staining, Mass Spectrometry