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  • 96
    Vector Laboratories biotinylated wisteria floribunda lectin (wfa, wfl)
    Biotinylated Wisteria Floribunda Lectin (Wfa, Wfl), supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated wisteria floribunda lectin (wfa, wfl)/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated wisteria floribunda lectin (wfa, wfl) - by Bioz Stars, 2024-06
    96/100 stars
      Buy from Supplier

    96
    Vector Laboratories biotinylated wfa
    <t>WFA</t> binding to sections of murine muscle . Transverse sections of quadriceps from wild type ( wt ) ( A ), mdx ( B ), α7 integrin null ( C ), or Akt-overexpressing ( Akt-DTg ) ( D ), were stained with <t>biotinylated</t> WFA, and bound lectin was detected with fluorescein-streptavidin using indirect immunofluorescence microscopy. Note punctuate staining in wild type and α7-null, compared with robust staining surrounding muscle cells in mdx and Akt-DTg mice. Bar , 50 μ m .
    Biotinylated Wfa, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated wfa/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated wfa - by Bioz Stars, 2024-06
    96/100 stars
      Buy from Supplier

    96
    Vector Laboratories b 1355
    <t>WFA</t> binding to sections of murine muscle . Transverse sections of quadriceps from wild type ( wt ) ( A ), mdx ( B ), α7 integrin null ( C ), or Akt-overexpressing ( Akt-DTg ) ( D ), were stained with <t>biotinylated</t> WFA, and bound lectin was detected with fluorescein-streptavidin using indirect immunofluorescence microscopy. Note punctuate staining in wild type and α7-null, compared with robust staining surrounding muscle cells in mdx and Akt-DTg mice. Bar , 50 μ m .
    B 1355, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/b 1355/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    b 1355 - by Bioz Stars, 2024-06
    96/100 stars
      Buy from Supplier

    96
    Vector Laboratories biotinylated wisteria floribunda lectin
    KEY RESOURCES TABLE
    Biotinylated Wisteria Floribunda Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated wisteria floribunda lectin/product/Vector Laboratories
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated wisteria floribunda lectin - by Bioz Stars, 2024-06
    96/100 stars
      Buy from Supplier

    Image Search Results


    WFA binding to sections of murine muscle . Transverse sections of quadriceps from wild type ( wt ) ( A ), mdx ( B ), α7 integrin null ( C ), or Akt-overexpressing ( Akt-DTg ) ( D ), were stained with biotinylated WFA, and bound lectin was detected with fluorescein-streptavidin using indirect immunofluorescence microscopy. Note punctuate staining in wild type and α7-null, compared with robust staining surrounding muscle cells in mdx and Akt-DTg mice. Bar , 50 μ m .

    Journal: The Journal of Biological Chemistry

    Article Title: High Throughput Screening for Compounds That Alter Muscle Cell Glycosylation Identifies New Role for N -Glycans in Regulating Sarcolemmal Protein Abundance and Laminin Binding

    doi: 10.1074/jbc.M111.334581

    Figure Lengend Snippet: WFA binding to sections of murine muscle . Transverse sections of quadriceps from wild type ( wt ) ( A ), mdx ( B ), α7 integrin null ( C ), or Akt-overexpressing ( Akt-DTg ) ( D ), were stained with biotinylated WFA, and bound lectin was detected with fluorescein-streptavidin using indirect immunofluorescence microscopy. Note punctuate staining in wild type and α7-null, compared with robust staining surrounding muscle cells in mdx and Akt-DTg mice. Bar , 50 μ m .

    Article Snippet: Biotinylated WFA (B-1355, Vector), 1:1500 in PBS, was added to sections at 4 °C overnight, and sections were washed three times with PBS.

    Techniques: Binding Assay, Staining, Immunofluorescence, Microscopy

    Lobeline treatment increases laminin binding. Lysates of surface biotinylated wild type ( wt ) ( A ) or mdx myotubes ( B ) treated with lobeline ( L ) or control ( C ) were separated by SDS-PAGE and blotted, laminin was incubated with the blots, and bound laminin was detected with anti-laminin. Blots were stripped and reprobed with mAb IIH6.

    Journal: The Journal of Biological Chemistry

    Article Title: High Throughput Screening for Compounds That Alter Muscle Cell Glycosylation Identifies New Role for N -Glycans in Regulating Sarcolemmal Protein Abundance and Laminin Binding

    doi: 10.1074/jbc.M111.334581

    Figure Lengend Snippet: Lobeline treatment increases laminin binding. Lysates of surface biotinylated wild type ( wt ) ( A ) or mdx myotubes ( B ) treated with lobeline ( L ) or control ( C ) were separated by SDS-PAGE and blotted, laminin was incubated with the blots, and bound laminin was detected with anti-laminin. Blots were stripped and reprobed with mAb IIH6.

    Article Snippet: Biotinylated WFA (B-1355, Vector), 1:1500 in PBS, was added to sections at 4 °C overnight, and sections were washed three times with PBS.

    Techniques: Binding Assay, SDS Page, Incubation

    Complex N -glycans are required for WFA binding. A , C2C12 myoblasts were treated with DMNJ or vehicle control, and binding of biotinylated PHA ( solid line ) was determined by flow cytometry, using biotinylated BSA as a control ( dotted line ). PHA binding was virtually eliminated by treatment with DMNJ, which inhibits mannosidase I and prevents conversion of high mannose N -glycans to complex N -glycans. B , loss of complex N -glycans abrogated WFA binding in cells treated with or without lobeline. Left , PHA binding determined in plate binding assay; PHA binding is not affected by lobeline ( L −) but is abolished by DMNJ in both control ( C +) and lobeline-treated ( L +) cells. Right , DMNJ treatment markedly abrogates WFA binding to cells treated with control ( C +) and lobeline ( L +), whereas cells bearing complex N -glycans demonstrate increased WFA binding after lobeline treatment ( L −). Data are mean ± S.D. ( error bars ) of six replicate samples from one of three independent experiments.

    Journal: The Journal of Biological Chemistry

    Article Title: High Throughput Screening for Compounds That Alter Muscle Cell Glycosylation Identifies New Role for N -Glycans in Regulating Sarcolemmal Protein Abundance and Laminin Binding

    doi: 10.1074/jbc.M111.334581

    Figure Lengend Snippet: Complex N -glycans are required for WFA binding. A , C2C12 myoblasts were treated with DMNJ or vehicle control, and binding of biotinylated PHA ( solid line ) was determined by flow cytometry, using biotinylated BSA as a control ( dotted line ). PHA binding was virtually eliminated by treatment with DMNJ, which inhibits mannosidase I and prevents conversion of high mannose N -glycans to complex N -glycans. B , loss of complex N -glycans abrogated WFA binding in cells treated with or without lobeline. Left , PHA binding determined in plate binding assay; PHA binding is not affected by lobeline ( L −) but is abolished by DMNJ in both control ( C +) and lobeline-treated ( L +) cells. Right , DMNJ treatment markedly abrogates WFA binding to cells treated with control ( C +) and lobeline ( L +), whereas cells bearing complex N -glycans demonstrate increased WFA binding after lobeline treatment ( L −). Data are mean ± S.D. ( error bars ) of six replicate samples from one of three independent experiments.

    Article Snippet: Biotinylated WFA (B-1355, Vector), 1:1500 in PBS, was added to sections at 4 °C overnight, and sections were washed three times with PBS.

    Techniques: Binding Assay, Flow Cytometry

    KEY RESOURCES TABLE

    Journal: Molecular cell

    Article Title: An Atlas of Human Glycosylation Pathways Enables Display of the Human Glycome by Gene Engineered Cells

    doi: 10.1016/j.molcel.2019.05.017

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Biotinylated Wisteria Floribunda Lectin (WFA, WFL) , Vector Laboratories , Cat#B-1355.

    Techniques: Recombinant, Plasmid Preparation, Construct, Software