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    MedChemExpress nicorandil treatment
    <t>Nicorandil</t> alleviates myocardial remodelling in diabetic rats. A: A1: gross morphology; A2: HE staining of cross shaft of musculi papillares in heart A3: HE staining longitudinal section; A4: HE staining of cross section; B: heart weight/bodyweight; C: cardiomyocyte cell diameter. DM: Diabetic mellitus. N7.5: nicorandil, 7.5 mg/kg·day; N15: nicorandil, 15 mg/kg·day. * P < 0.05 compared with control; # P < 0.05 compared with DM; Data are means ± SD
    Nicorandil Treatment, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nicorandil treatment/product/MedChemExpress
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    nicorandil treatment - by Bioz Stars, 2024-03
    92/100 stars
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    92
    Echelon Biosciences 3 a aminocholestane
    A–C A mass ELISA was used to detect specific PIP species after exposure to anti‐FcγRII/III (2.4G2) with or without <t>3‐a‐aminocholestane</t> (SHIP 1 inhibitor), SF1670 (PTEN inhibitor) and LY294002 (PI‐3K inhibitor). In M‐MOP cells (left panel) and primary microglia (right panel), PI(4,5)P 2 (A), PI(3,4)P 2 (B) and PIP 3 (C) were detected. All data show the mean ± SD of three independent experiments and were analysed by two‐way ANOVA (log‐transformed data were used in C) with Sidak’s multiple comparison tests performed. * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001 (blue: P522 and red: R522).
    3 A Aminocholestane, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/3 a aminocholestane/product/Echelon Biosciences
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    3 a aminocholestane - by Bioz Stars, 2024-03
    92/100 stars
      Buy from Supplier

    Image Search Results


    Nicorandil alleviates myocardial remodelling in diabetic rats. A: A1: gross morphology; A2: HE staining of cross shaft of musculi papillares in heart A3: HE staining longitudinal section; A4: HE staining of cross section; B: heart weight/bodyweight; C: cardiomyocyte cell diameter. DM: Diabetic mellitus. N7.5: nicorandil, 7.5 mg/kg·day; N15: nicorandil, 15 mg/kg·day. * P < 0.05 compared with control; # P < 0.05 compared with DM; Data are means ± SD

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Nicorandil alleviates apoptosis in diabetic cardiomyopathy through PI3K/Akt pathway

    doi: 10.1111/jcmm.14413

    Figure Lengend Snippet: Nicorandil alleviates myocardial remodelling in diabetic rats. A: A1: gross morphology; A2: HE staining of cross shaft of musculi papillares in heart A3: HE staining longitudinal section; A4: HE staining of cross section; B: heart weight/bodyweight; C: cardiomyocyte cell diameter. DM: Diabetic mellitus. N7.5: nicorandil, 7.5 mg/kg·day; N15: nicorandil, 15 mg/kg·day. * P < 0.05 compared with control; # P < 0.05 compared with DM; Data are means ± SD

    Article Snippet: Miltefosine (MTF, MCE, USA) and rapamycin (Rapa, MCE, USA) were used to inhibit PI3K/Akt/mTOR pathway at the time of nicorandil treatment in HG stimulation for 24 hr.

    Techniques: Staining

    Nicorandil alleviates cardiac dysfunction in diabetic rats. A1: representive 2D echocardiograms. A2: representative M‐mode echocardiograms. A3: representative pulse‐wave Doppler echocardiograms of mitral inflow. A4: representative tissue Doppler echocardiograms. B1: Left ventricle ejection fraction (LVEF). B2: Fractional shortening (FS). B3: Early to late mitral flow (E/A). B4: Ratio of diastolic mitral annulus velocities (e’/a’). B5: E/e’. B6: Left ventricle end‐diastolic dimension (LVEDd). DM: Diabetic mellitus. N7.5: nicorandil, 7.5 mg/kg·day; N15: nicorandil, 15 mg/kg·day. * P < 0.05 compared with control; # P < 0.05 compared with DM; Data are means ± SD

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Nicorandil alleviates apoptosis in diabetic cardiomyopathy through PI3K/Akt pathway

    doi: 10.1111/jcmm.14413

    Figure Lengend Snippet: Nicorandil alleviates cardiac dysfunction in diabetic rats. A1: representive 2D echocardiograms. A2: representative M‐mode echocardiograms. A3: representative pulse‐wave Doppler echocardiograms of mitral inflow. A4: representative tissue Doppler echocardiograms. B1: Left ventricle ejection fraction (LVEF). B2: Fractional shortening (FS). B3: Early to late mitral flow (E/A). B4: Ratio of diastolic mitral annulus velocities (e’/a’). B5: E/e’. B6: Left ventricle end‐diastolic dimension (LVEDd). DM: Diabetic mellitus. N7.5: nicorandil, 7.5 mg/kg·day; N15: nicorandil, 15 mg/kg·day. * P < 0.05 compared with control; # P < 0.05 compared with DM; Data are means ± SD

    Article Snippet: Miltefosine (MTF, MCE, USA) and rapamycin (Rapa, MCE, USA) were used to inhibit PI3K/Akt/mTOR pathway at the time of nicorandil treatment in HG stimulation for 24 hr.

    Techniques:

    Nicorandil alleviate cardiac fibrosis in type 2 diabetic rat. A: Masson's trichrome staining and Picorosirius Red staining of myocardium. Immunohistochemical staining of Collagen I and Collagen III; B: Western blot analysis of MMP2, MMP9, Collagen I and Collagen III. DM: Diabetic mellitus, N7.5: nicorandil, 7.5 mg/kg·day; N15: nicorandil, 15 mg/kg·day. * P < 0.05 compared with control; # P < 0.05 compared with DM; # P < 0.05 compared with HG + N, Data are means ± SD

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Nicorandil alleviates apoptosis in diabetic cardiomyopathy through PI3K/Akt pathway

    doi: 10.1111/jcmm.14413

    Figure Lengend Snippet: Nicorandil alleviate cardiac fibrosis in type 2 diabetic rat. A: Masson's trichrome staining and Picorosirius Red staining of myocardium. Immunohistochemical staining of Collagen I and Collagen III; B: Western blot analysis of MMP2, MMP9, Collagen I and Collagen III. DM: Diabetic mellitus, N7.5: nicorandil, 7.5 mg/kg·day; N15: nicorandil, 15 mg/kg·day. * P < 0.05 compared with control; # P < 0.05 compared with DM; # P < 0.05 compared with HG + N, Data are means ± SD

    Article Snippet: Miltefosine (MTF, MCE, USA) and rapamycin (Rapa, MCE, USA) were used to inhibit PI3K/Akt/mTOR pathway at the time of nicorandil treatment in HG stimulation for 24 hr.

    Techniques: Staining, Immunohistochemical staining, Western Blot

    Nicorandil alleviates cardiac apoptosis in type 2 diabetic rat. A: TUNEL staining and TUNEL‐positive cells rate. B: Western blot analysis of Bax/Bcl‐2 and cleaved caspase‐3. C: Level of nitric oxide and ADMA in serum. D: Western blot analysis of p‐eNOS. DM: Diabetic mellitus, N7.5: nicorandil, 7.5 mg/kg·day; N15: nicorandil, 15 mg/kg·day. * P < 0.05 compared with control; # P < 0.05 compared with DM; # P < 0.05 compared with HG + N, Data are means ± SD

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Nicorandil alleviates apoptosis in diabetic cardiomyopathy through PI3K/Akt pathway

    doi: 10.1111/jcmm.14413

    Figure Lengend Snippet: Nicorandil alleviates cardiac apoptosis in type 2 diabetic rat. A: TUNEL staining and TUNEL‐positive cells rate. B: Western blot analysis of Bax/Bcl‐2 and cleaved caspase‐3. C: Level of nitric oxide and ADMA in serum. D: Western blot analysis of p‐eNOS. DM: Diabetic mellitus, N7.5: nicorandil, 7.5 mg/kg·day; N15: nicorandil, 15 mg/kg·day. * P < 0.05 compared with control; # P < 0.05 compared with DM; # P < 0.05 compared with HG + N, Data are means ± SD

    Article Snippet: Miltefosine (MTF, MCE, USA) and rapamycin (Rapa, MCE, USA) were used to inhibit PI3K/Akt/mTOR pathway at the time of nicorandil treatment in HG stimulation for 24 hr.

    Techniques: TUNEL Assay, Staining, Western Blot

    Apoptosis level reduced after nicorandil treatment in high glucose‐induced H9c2 cardiomyocyte. A: Western blot analysis of bax and bcl‐2 in high glucose‐induced H9c2 cardiomyocyte after nicorandil treatment with different concentrations for 24 h. B: Western bolt analysis of cleaved caspase‐3 in high glucose‐induced H9c2 cardiomyocyte after nicorandil treatment. HG (33.3 mmol/L), NG (5.5 mmol/L), n1: nicorandil (10 µmol); n2: nicorandil (50 µmol); n3: nicorandil (100 µmol). # P < 0.05 compared with NG; * P < 0.05 compared with HG, Data are means ± SD

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Nicorandil alleviates apoptosis in diabetic cardiomyopathy through PI3K/Akt pathway

    doi: 10.1111/jcmm.14413

    Figure Lengend Snippet: Apoptosis level reduced after nicorandil treatment in high glucose‐induced H9c2 cardiomyocyte. A: Western blot analysis of bax and bcl‐2 in high glucose‐induced H9c2 cardiomyocyte after nicorandil treatment with different concentrations for 24 h. B: Western bolt analysis of cleaved caspase‐3 in high glucose‐induced H9c2 cardiomyocyte after nicorandil treatment. HG (33.3 mmol/L), NG (5.5 mmol/L), n1: nicorandil (10 µmol); n2: nicorandil (50 µmol); n3: nicorandil (100 µmol). # P < 0.05 compared with NG; * P < 0.05 compared with HG, Data are means ± SD

    Article Snippet: Miltefosine (MTF, MCE, USA) and rapamycin (Rapa, MCE, USA) were used to inhibit PI3K/Akt/mTOR pathway at the time of nicorandil treatment in HG stimulation for 24 hr.

    Techniques: Western Blot

    Nicorandil protects H9C2 cells from apoptosis through PI3K/AKT pathway. A: Western blot analysis of Bax/Bcl‐2 and cleaved caspase‐3 in high glucose‐induced H9c2 cardiomyocyte after nicorandil treatment or both nicorandil treatment and 5‐HD which is a inhibitor of nicorandil. B: TUNEL assay of apoptosis rate of high glucose‐induced H9c2 cardiomyocyte after nicorandil treatment or both nicorandil treatment and nicorandil inhibitor(5‐HD, 500 µmol) (scale bar: 20 µm). I:NG, II:HG, III:HG + N, IV:HG + N+5‐HD; C: Western blot analysis of phosphorylation level of PI3K, AKT, eNOS and mTOR in high glucose‐induced H9c2 cardiomyocyte after nicorandil treatment or both nicorandil treatment and nicorandil inhibitor(5‐HD). N: Nicorandil (100 µmol); NG: normal glucose (5.5 mmol/L); HG: high glucose (25 mmol/L). * P < 0.05 compared with NG; # P < 0.05 compared with HG; & P < 0.05 compared with HG + N, Data are means ± SD

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Nicorandil alleviates apoptosis in diabetic cardiomyopathy through PI3K/Akt pathway

    doi: 10.1111/jcmm.14413

    Figure Lengend Snippet: Nicorandil protects H9C2 cells from apoptosis through PI3K/AKT pathway. A: Western blot analysis of Bax/Bcl‐2 and cleaved caspase‐3 in high glucose‐induced H9c2 cardiomyocyte after nicorandil treatment or both nicorandil treatment and 5‐HD which is a inhibitor of nicorandil. B: TUNEL assay of apoptosis rate of high glucose‐induced H9c2 cardiomyocyte after nicorandil treatment or both nicorandil treatment and nicorandil inhibitor(5‐HD, 500 µmol) (scale bar: 20 µm). I:NG, II:HG, III:HG + N, IV:HG + N+5‐HD; C: Western blot analysis of phosphorylation level of PI3K, AKT, eNOS and mTOR in high glucose‐induced H9c2 cardiomyocyte after nicorandil treatment or both nicorandil treatment and nicorandil inhibitor(5‐HD). N: Nicorandil (100 µmol); NG: normal glucose (5.5 mmol/L); HG: high glucose (25 mmol/L). * P < 0.05 compared with NG; # P < 0.05 compared with HG; & P < 0.05 compared with HG + N, Data are means ± SD

    Article Snippet: Miltefosine (MTF, MCE, USA) and rapamycin (Rapa, MCE, USA) were used to inhibit PI3K/Akt/mTOR pathway at the time of nicorandil treatment in HG stimulation for 24 hr.

    Techniques: Western Blot, TUNEL Assay

    PI3K/AKT pathway inhibition blocked the protection of nicorandil on H9c2 cardiomyocyte treated with high glucose. A: Western blot analysis of Bax/Bcl‐2 and cleaved caspase‐3 in high glucose‐induced H9c2 cardiomyocyte after nicorandil treatment or both nicorandil treatment and PI3K/mTOR inhibitors. B: TUNEL assay of apoptosis rate of high glucose‐induced H9c2 cardiomyocyte after nicorandil treatment or both nicorandil treatment and PI3K/mTOR inhibitors (scale bar: 20 µm). C: Western blot analysis of p‐eNOS in high glucose‐induced H9c2 cardiomyocyte after nicorandil treatment or both nicorandil treatment and PI3K/mTOR inhibitors. N: Nicorandil (100 µmol); MTF: miltefosine (100 µmol); Rapa: rapamycin (100 µmol) NG: normal glucose (5.5 mmol/L); HG: high glucose (25 mmol/L). * P < 0.05 compared with NG; # P < 0.05 compared with HG; & P < 0.05 compared with HG + N, Data are means ± SD

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Nicorandil alleviates apoptosis in diabetic cardiomyopathy through PI3K/Akt pathway

    doi: 10.1111/jcmm.14413

    Figure Lengend Snippet: PI3K/AKT pathway inhibition blocked the protection of nicorandil on H9c2 cardiomyocyte treated with high glucose. A: Western blot analysis of Bax/Bcl‐2 and cleaved caspase‐3 in high glucose‐induced H9c2 cardiomyocyte after nicorandil treatment or both nicorandil treatment and PI3K/mTOR inhibitors. B: TUNEL assay of apoptosis rate of high glucose‐induced H9c2 cardiomyocyte after nicorandil treatment or both nicorandil treatment and PI3K/mTOR inhibitors (scale bar: 20 µm). C: Western blot analysis of p‐eNOS in high glucose‐induced H9c2 cardiomyocyte after nicorandil treatment or both nicorandil treatment and PI3K/mTOR inhibitors. N: Nicorandil (100 µmol); MTF: miltefosine (100 µmol); Rapa: rapamycin (100 µmol) NG: normal glucose (5.5 mmol/L); HG: high glucose (25 mmol/L). * P < 0.05 compared with NG; # P < 0.05 compared with HG; & P < 0.05 compared with HG + N, Data are means ± SD

    Article Snippet: Miltefosine (MTF, MCE, USA) and rapamycin (Rapa, MCE, USA) were used to inhibit PI3K/Akt/mTOR pathway at the time of nicorandil treatment in HG stimulation for 24 hr.

    Techniques: Inhibition, Western Blot, TUNEL Assay

    A–C A mass ELISA was used to detect specific PIP species after exposure to anti‐FcγRII/III (2.4G2) with or without 3‐a‐aminocholestane (SHIP 1 inhibitor), SF1670 (PTEN inhibitor) and LY294002 (PI‐3K inhibitor). In M‐MOP cells (left panel) and primary microglia (right panel), PI(4,5)P 2 (A), PI(3,4)P 2 (B) and PIP 3 (C) were detected. All data show the mean ± SD of three independent experiments and were analysed by two‐way ANOVA (log‐transformed data were used in C) with Sidak’s multiple comparison tests performed. * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001 (blue: P522 and red: R522).

    Journal: The EMBO Journal

    Article Title: PIP2 depletion and altered endocytosis caused by expression of Alzheimer's disease‐protective variant PLCγ2 R522

    doi: 10.15252/embj.2020105603

    Figure Lengend Snippet: A–C A mass ELISA was used to detect specific PIP species after exposure to anti‐FcγRII/III (2.4G2) with or without 3‐a‐aminocholestane (SHIP 1 inhibitor), SF1670 (PTEN inhibitor) and LY294002 (PI‐3K inhibitor). In M‐MOP cells (left panel) and primary microglia (right panel), PI(4,5)P 2 (A), PI(3,4)P 2 (B) and PIP 3 (C) were detected. All data show the mean ± SD of three independent experiments and were analysed by two‐way ANOVA (log‐transformed data were used in C) with Sidak’s multiple comparison tests performed. * P < 0.05, ** P < 0.01, *** P < 0.001 and **** P < 0.0001 (blue: P522 and red: R522).

    Article Snippet: Designated wells were inhibited by pre‐exposure for 2 h with 3‐a‐aminocholestane (20 µM, B‐0341), LY294002 (10 µM, B‐0294) or SF1670 (5 µM, B‐0350) (Echelon Biosciences).

    Techniques: Enzyme-linked Immunosorbent Assay, Transformation Assay