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  • 94
    MedChemExpress flubendazole flu
    Flubendazole Flu, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/flubendazole flu/product/MedChemExpress
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    93
    Echelon Biosciences ly294002
    A . Fura2 340/380 time traces from M-CSF-differentiated macrophages derived from conditionally-immortalised macrophage precursor cell lines (M-MØP) of Plcg2 P522 mice (P522, blue) and Plcg2 R522 mice (R522, red). One set of cell lines, generated from male mice, is shown. Cells were exposed to 5µg/ml anti-FcγRII/III along with 20µM EGTA and 2µM Ionomycin as indicated. B . Fura2 340/380 time traces from primary microglia derived from the cortex of Plcg2 R522 mice (blue: P522) and Plcg2 R522 mice (red: R522) with or without pre-exposure for 2 hours with Edelfosine <t>(10µM).</t> Cells were exposed to 5µg/ml anti-FcγRII/III along with EGTA and 2µM Ionomycin. Data in A and B shows the mean±SD of 3 independent experiments analysed by two-way ANOVA with Sidak post-hoc tests. PLCG2 R522 hiPSC-derived microglia show increased cytosolic Ca 2+ influx in comparison to controls following activation of PLCγ2 using anti-CD32. Cytoplasmic Ca2+ increase following activation of PLCγ2 using anti-CD32 Representative Ca 2+ traces ( C ) and graphical summary ( D ). 3 independent PLCG2 R522 CRISPR-engineered clones were examined. Data shown is mean±SD of 4 independent experiments and were analysed by one-way ANOVA with Tukey’s multiple comparison test (** = p<0.01; *** = p<0.001; **** = p< 0.0001).
    Ly294002, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    A . Fura2 340/380 time traces from M-CSF-differentiated macrophages derived from conditionally-immortalised macrophage precursor cell lines (M-MØP) of Plcg2 P522 mice (P522, blue) and Plcg2 R522 mice (R522, red). One set of cell lines, generated from male mice, is shown. Cells were exposed to 5µg/ml anti-FcγRII/III along with 20µM EGTA and 2µM Ionomycin as indicated. B . Fura2 340/380 time traces from primary microglia derived from the cortex of Plcg2 R522 mice (blue: P522) and Plcg2 R522 mice (red: R522) with or without pre-exposure for 2 hours with Edelfosine (10µM). Cells were exposed to 5µg/ml anti-FcγRII/III along with EGTA and 2µM Ionomycin. Data in A and B shows the mean±SD of 3 independent experiments analysed by two-way ANOVA with Sidak post-hoc tests. PLCG2 R522 hiPSC-derived microglia show increased cytosolic Ca 2+ influx in comparison to controls following activation of PLCγ2 using anti-CD32. Cytoplasmic Ca2+ increase following activation of PLCγ2 using anti-CD32 Representative Ca 2+ traces ( C ) and graphical summary ( D ). 3 independent PLCG2 R522 CRISPR-engineered clones were examined. Data shown is mean±SD of 4 independent experiments and were analysed by one-way ANOVA with Tukey’s multiple comparison test (** = p<0.01; *** = p<0.001; **** = p< 0.0001).

    Journal: bioRxiv

    Article Title: The Alzheimer’s disease protective P522R variant of PLCG2 , consistently enhances stimulus-dependent PLCγ2 activation, depleting substrate and altering cell function

    doi: 10.1101/2020.04.27.059600

    Figure Lengend Snippet: A . Fura2 340/380 time traces from M-CSF-differentiated macrophages derived from conditionally-immortalised macrophage precursor cell lines (M-MØP) of Plcg2 P522 mice (P522, blue) and Plcg2 R522 mice (R522, red). One set of cell lines, generated from male mice, is shown. Cells were exposed to 5µg/ml anti-FcγRII/III along with 20µM EGTA and 2µM Ionomycin as indicated. B . Fura2 340/380 time traces from primary microglia derived from the cortex of Plcg2 R522 mice (blue: P522) and Plcg2 R522 mice (red: R522) with or without pre-exposure for 2 hours with Edelfosine (10µM). Cells were exposed to 5µg/ml anti-FcγRII/III along with EGTA and 2µM Ionomycin. Data in A and B shows the mean±SD of 3 independent experiments analysed by two-way ANOVA with Sidak post-hoc tests. PLCG2 R522 hiPSC-derived microglia show increased cytosolic Ca 2+ influx in comparison to controls following activation of PLCγ2 using anti-CD32. Cytoplasmic Ca2+ increase following activation of PLCγ2 using anti-CD32 Representative Ca 2+ traces ( C ) and graphical summary ( D ). 3 independent PLCG2 R522 CRISPR-engineered clones were examined. Data shown is mean±SD of 4 independent experiments and were analysed by one-way ANOVA with Tukey’s multiple comparison test (** = p<0.01; *** = p<0.001; **** = p< 0.0001).

    Article Snippet: Designated wells were inhibited by pre exposure for 2 hours with 3-a-aminocholestane (20µM, B-0341), LY294002 (10µM, B-0294) or SF1670 (5µM, B-0350) (Echelon Bioscience).

    Techniques: Derivative Assay, Generated, Activation Assay, CRISPR, Clone Assay

    A . M-MØP (blue: P522; red: R522) were loaded with Fluo-8 Ca 2+ indicator and examined for peak changes in fluoresce after exposure to 5ug/ml anti-FcγRII/III (2.4G2) with or without pre-exposure for 2 hours with Edelfosine (10µM) or U73122 (5µM) or Xestospogin C (5µM). B . Left panel shows LNA GapmeR percentage knockdown of Plcg2 gene expression in M-MOP cells within the P522 control (blue) and R522 (red) variant lines. Right panel shows Fura2 340/380 time traces from M-MØP cell lines (blue: P522; red: R522) which have undergone Plcg2 knockdown (square symbols) using an antisense LNA GapmeR. Cells were exposed to 5µg/ml anti-FcγRII/III and 2µM Ionomycin. C . Microglia from Plcg2 P522 (blue: P522) mice and Plcg2 R522 mice (red: R522) from neonate cortex (solid colour) or hippocampus (striped) were loaded with Fluo-8 Ca 2+ indicator. These cells were then examined for peak changes in fluoresce after exposure to 5µg/ml anti-FcγRII/III. These readings were taken with or without pre-exposure for 2 hours with Edelfosine (10µM) or U73122 (5µM). D Cortical microglia from Plcg2 P522 (blue: P522) mice and Plcg2 R522 mice (red: R522) from neonate cortex (solid colour) or hippocampus (striped) were loaded with Fluo-8 Ca 2+ indicator. These cells were then examined for peak changes in fluoresce after exposure to LPS (50ng/ml) or Aβ 1-42 oligomers (40µM). Data shown as the mean±SD of 3 independent experiments. Data in A, C and D were analysed by two-way ANOVA with Dunnett’s multiple Comparison test. See also Supplementary figure 3.

    Journal: bioRxiv

    Article Title: The Alzheimer’s disease protective P522R variant of PLCG2 , consistently enhances stimulus-dependent PLCγ2 activation, depleting substrate and altering cell function

    doi: 10.1101/2020.04.27.059600

    Figure Lengend Snippet: A . M-MØP (blue: P522; red: R522) were loaded with Fluo-8 Ca 2+ indicator and examined for peak changes in fluoresce after exposure to 5ug/ml anti-FcγRII/III (2.4G2) with or without pre-exposure for 2 hours with Edelfosine (10µM) or U73122 (5µM) or Xestospogin C (5µM). B . Left panel shows LNA GapmeR percentage knockdown of Plcg2 gene expression in M-MOP cells within the P522 control (blue) and R522 (red) variant lines. Right panel shows Fura2 340/380 time traces from M-MØP cell lines (blue: P522; red: R522) which have undergone Plcg2 knockdown (square symbols) using an antisense LNA GapmeR. Cells were exposed to 5µg/ml anti-FcγRII/III and 2µM Ionomycin. C . Microglia from Plcg2 P522 (blue: P522) mice and Plcg2 R522 mice (red: R522) from neonate cortex (solid colour) or hippocampus (striped) were loaded with Fluo-8 Ca 2+ indicator. These cells were then examined for peak changes in fluoresce after exposure to 5µg/ml anti-FcγRII/III. These readings were taken with or without pre-exposure for 2 hours with Edelfosine (10µM) or U73122 (5µM). D Cortical microglia from Plcg2 P522 (blue: P522) mice and Plcg2 R522 mice (red: R522) from neonate cortex (solid colour) or hippocampus (striped) were loaded with Fluo-8 Ca 2+ indicator. These cells were then examined for peak changes in fluoresce after exposure to LPS (50ng/ml) or Aβ 1-42 oligomers (40µM). Data shown as the mean±SD of 3 independent experiments. Data in A, C and D were analysed by two-way ANOVA with Dunnett’s multiple Comparison test. See also Supplementary figure 3.

    Article Snippet: Designated wells were inhibited by pre exposure for 2 hours with 3-a-aminocholestane (20µM, B-0341), LY294002 (10µM, B-0294) or SF1670 (5µM, B-0350) (Echelon Bioscience).

    Techniques: Expressing, Variant Assay

    A mass ELISA was used to detect specific PIP species after exposure to anti-FcγRII/III (2.4G2) with or without 3-a-aminocholestane (SHIP 1 inhibitor), SF1670 (PTEN inhibitor) and LY294002 (PI-3K inhibitor). In M-MOP cells (left panel) and primary microglia (right panel) PI(4,5)P 2 (A) , PI(3,4)P 2 (B) and PIP 3 (C) were detected. All data shows the mean±SD of 3 independent experiments were analysed by 2 way ANOVA (log-transformed data was used in C) with Sidak’s multiple comparison tests performed *=p<0.05, **=p<0.01, ***=p<0.001, ****=p<0.0001. (blue: P522; red: R522)

    Journal: bioRxiv

    Article Title: The Alzheimer’s disease protective P522R variant of PLCG2 , consistently enhances stimulus-dependent PLCγ2 activation, depleting substrate and altering cell function

    doi: 10.1101/2020.04.27.059600

    Figure Lengend Snippet: A mass ELISA was used to detect specific PIP species after exposure to anti-FcγRII/III (2.4G2) with or without 3-a-aminocholestane (SHIP 1 inhibitor), SF1670 (PTEN inhibitor) and LY294002 (PI-3K inhibitor). In M-MOP cells (left panel) and primary microglia (right panel) PI(4,5)P 2 (A) , PI(3,4)P 2 (B) and PIP 3 (C) were detected. All data shows the mean±SD of 3 independent experiments were analysed by 2 way ANOVA (log-transformed data was used in C) with Sidak’s multiple comparison tests performed *=p<0.05, **=p<0.01, ***=p<0.001, ****=p<0.0001. (blue: P522; red: R522)

    Article Snippet: Designated wells were inhibited by pre exposure for 2 hours with 3-a-aminocholestane (20µM, B-0341), LY294002 (10µM, B-0294) or SF1670 (5µM, B-0350) (Echelon Bioscience).

    Techniques: Enzyme-linked Immunosorbent Assay, Transformation Assay