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  • 91
    Carl Zeiss axio imager a1
    Effects of anti-HER2 antibody BH1 and tumor necrosis factor-α on DNA synthesis and cell cycle in SK-BR-3 cells. ( A ) Effects on DNA synthesis were tested by 24-h BrdU incorporation assay. Cells were plated on coverslips in 12-well plates, treated as described in Figure 5 , and were exposed to BrdU during 24 h prior to indicated days. Nuclear BrdU was detected by indirect immunofluorescence assay. Nuclei were counterstained with DAPI, examined using Zeiss <t>Axio</t> Imager.A1 microscope. Representative photographs (3–5 frames per coverslip) were acquired. Percent BrdU incorporation was calculated after manual counting of BrdU + and BrdU - nuclei. * Denotes p-values ≤ 0.05 (BH1 vs. IgG1, BH1 + TNF-α vs. IgG1 + TNF-α). T-bars: SD. ( B - D ). Effects on cell cycle were analyzed by flow cytometry. SK-BR-3 cells were treated with 20 μg/ml BH1 or IgG isotype control antibody in the presence or absence of 1000 U/ml TNF-α. Cells were fixed on day 1 ( B ), day 3 ( C ) and day 6 ( D ), stained with propidium iodide and the cell cycle distribution was analysed with flow cytometry. Fraction of cells present in G2/M-phase (G2/M), S-phase (S) and G1-phase (G1) are shown in purple, green and orange respectively. Fraction of apoptotic cells observed as a SubG1 peak is shown in black.
    Axio Imager A1, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 91/100, based on 893 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Carl Zeiss axio imager a1 optical microscope
    Effects of anti-HER2 antibody BH1 and tumor necrosis factor-α on DNA synthesis and cell cycle in SK-BR-3 cells. ( A ) Effects on DNA synthesis were tested by 24-h BrdU incorporation assay. Cells were plated on coverslips in 12-well plates, treated as described in Figure 5 , and were exposed to BrdU during 24 h prior to indicated days. Nuclear BrdU was detected by indirect immunofluorescence assay. Nuclei were counterstained with DAPI, examined using Zeiss <t>Axio</t> Imager.A1 microscope. Representative photographs (3–5 frames per coverslip) were acquired. Percent BrdU incorporation was calculated after manual counting of BrdU + and BrdU - nuclei. * Denotes p-values ≤ 0.05 (BH1 vs. IgG1, BH1 + TNF-α vs. IgG1 + TNF-α). T-bars: SD. ( B - D ). Effects on cell cycle were analyzed by flow cytometry. SK-BR-3 cells were treated with 20 μg/ml BH1 or IgG isotype control antibody in the presence or absence of 1000 U/ml TNF-α. Cells were fixed on day 1 ( B ), day 3 ( C ) and day 6 ( D ), stained with propidium iodide and the cell cycle distribution was analysed with flow cytometry. Fraction of cells present in G2/M-phase (G2/M), S-phase (S) and G1-phase (G1) are shown in purple, green and orange respectively. Fraction of apoptotic cells observed as a SubG1 peak is shown in black.
    Axio Imager A1 Optical Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Carl Zeiss axio imager a1 fluorescent microscope
    Effects of anti-HER2 antibody BH1 and tumor necrosis factor-α on DNA synthesis and cell cycle in SK-BR-3 cells. ( A ) Effects on DNA synthesis were tested by 24-h BrdU incorporation assay. Cells were plated on coverslips in 12-well plates, treated as described in Figure 5 , and were exposed to BrdU during 24 h prior to indicated days. Nuclear BrdU was detected by indirect immunofluorescence assay. Nuclei were counterstained with DAPI, examined using Zeiss <t>Axio</t> Imager.A1 microscope. Representative photographs (3–5 frames per coverslip) were acquired. Percent BrdU incorporation was calculated after manual counting of BrdU + and BrdU - nuclei. * Denotes p-values ≤ 0.05 (BH1 vs. IgG1, BH1 + TNF-α vs. IgG1 + TNF-α). T-bars: SD. ( B - D ). Effects on cell cycle were analyzed by flow cytometry. SK-BR-3 cells were treated with 20 μg/ml BH1 or IgG isotype control antibody in the presence or absence of 1000 U/ml TNF-α. Cells were fixed on day 1 ( B ), day 3 ( C ) and day 6 ( D ), stained with propidium iodide and the cell cycle distribution was analysed with flow cytometry. Fraction of cells present in G2/M-phase (G2/M), S-phase (S) and G1-phase (G1) are shown in purple, green and orange respectively. Fraction of apoptotic cells observed as a SubG1 peak is shown in black.
    Axio Imager A1 Fluorescent Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 127 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Carl Zeiss axio imager a1 fluorescence microscope
    Effects of anti-HER2 antibody BH1 and tumor necrosis factor-α on DNA synthesis and cell cycle in SK-BR-3 cells. ( A ) Effects on DNA synthesis were tested by 24-h BrdU incorporation assay. Cells were plated on coverslips in 12-well plates, treated as described in Figure 5 , and were exposed to BrdU during 24 h prior to indicated days. Nuclear BrdU was detected by indirect immunofluorescence assay. Nuclei were counterstained with DAPI, examined using Zeiss <t>Axio</t> Imager.A1 microscope. Representative photographs (3–5 frames per coverslip) were acquired. Percent BrdU incorporation was calculated after manual counting of BrdU + and BrdU - nuclei. * Denotes p-values ≤ 0.05 (BH1 vs. IgG1, BH1 + TNF-α vs. IgG1 + TNF-α). T-bars: SD. ( B - D ). Effects on cell cycle were analyzed by flow cytometry. SK-BR-3 cells were treated with 20 μg/ml BH1 or IgG isotype control antibody in the presence or absence of 1000 U/ml TNF-α. Cells were fixed on day 1 ( B ), day 3 ( C ) and day 6 ( D ), stained with propidium iodide and the cell cycle distribution was analysed with flow cytometry. Fraction of cells present in G2/M-phase (G2/M), S-phase (S) and G1-phase (G1) are shown in purple, green and orange respectively. Fraction of apoptotic cells observed as a SubG1 peak is shown in black.
    Axio Imager A1 Fluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 492 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Carl Zeiss axio imager a1 epifluorescence microscope
    Effects of anti-HER2 antibody BH1 and tumor necrosis factor-α on DNA synthesis and cell cycle in SK-BR-3 cells. ( A ) Effects on DNA synthesis were tested by 24-h BrdU incorporation assay. Cells were plated on coverslips in 12-well plates, treated as described in Figure 5 , and were exposed to BrdU during 24 h prior to indicated days. Nuclear BrdU was detected by indirect immunofluorescence assay. Nuclei were counterstained with DAPI, examined using Zeiss <t>Axio</t> Imager.A1 microscope. Representative photographs (3–5 frames per coverslip) were acquired. Percent BrdU incorporation was calculated after manual counting of BrdU + and BrdU - nuclei. * Denotes p-values ≤ 0.05 (BH1 vs. IgG1, BH1 + TNF-α vs. IgG1 + TNF-α). T-bars: SD. ( B - D ). Effects on cell cycle were analyzed by flow cytometry. SK-BR-3 cells were treated with 20 μg/ml BH1 or IgG isotype control antibody in the presence or absence of 1000 U/ml TNF-α. Cells were fixed on day 1 ( B ), day 3 ( C ) and day 6 ( D ), stained with propidium iodide and the cell cycle distribution was analysed with flow cytometry. Fraction of cells present in G2/M-phase (G2/M), S-phase (S) and G1-phase (G1) are shown in purple, green and orange respectively. Fraction of apoptotic cells observed as a SubG1 peak is shown in black.
    Axio Imager A1 Epifluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 89/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Carl Zeiss axio imager a1 light microscope
    Effects of anti-HER2 antibody BH1 and tumor necrosis factor-α on DNA synthesis and cell cycle in SK-BR-3 cells. ( A ) Effects on DNA synthesis were tested by 24-h BrdU incorporation assay. Cells were plated on coverslips in 12-well plates, treated as described in Figure 5 , and were exposed to BrdU during 24 h prior to indicated days. Nuclear BrdU was detected by indirect immunofluorescence assay. Nuclei were counterstained with DAPI, examined using Zeiss <t>Axio</t> Imager.A1 microscope. Representative photographs (3–5 frames per coverslip) were acquired. Percent BrdU incorporation was calculated after manual counting of BrdU + and BrdU - nuclei. * Denotes p-values ≤ 0.05 (BH1 vs. IgG1, BH1 + TNF-α vs. IgG1 + TNF-α). T-bars: SD. ( B - D ). Effects on cell cycle were analyzed by flow cytometry. SK-BR-3 cells were treated with 20 μg/ml BH1 or IgG isotype control antibody in the presence or absence of 1000 U/ml TNF-α. Cells were fixed on day 1 ( B ), day 3 ( C ) and day 6 ( D ), stained with propidium iodide and the cell cycle distribution was analysed with flow cytometry. Fraction of cells present in G2/M-phase (G2/M), S-phase (S) and G1-phase (G1) are shown in purple, green and orange respectively. Fraction of apoptotic cells observed as a SubG1 peak is shown in black.
    Axio Imager A1 Light Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 88/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Carl Zeiss axio imager a1 microscopy system
    Effects of anti-HER2 antibody BH1 and tumor necrosis factor-α on DNA synthesis and cell cycle in SK-BR-3 cells. ( A ) Effects on DNA synthesis were tested by 24-h BrdU incorporation assay. Cells were plated on coverslips in 12-well plates, treated as described in Figure 5 , and were exposed to BrdU during 24 h prior to indicated days. Nuclear BrdU was detected by indirect immunofluorescence assay. Nuclei were counterstained with DAPI, examined using Zeiss <t>Axio</t> Imager.A1 microscope. Representative photographs (3–5 frames per coverslip) were acquired. Percent BrdU incorporation was calculated after manual counting of BrdU + and BrdU - nuclei. * Denotes p-values ≤ 0.05 (BH1 vs. IgG1, BH1 + TNF-α vs. IgG1 + TNF-α). T-bars: SD. ( B - D ). Effects on cell cycle were analyzed by flow cytometry. SK-BR-3 cells were treated with 20 μg/ml BH1 or IgG isotype control antibody in the presence or absence of 1000 U/ml TNF-α. Cells were fixed on day 1 ( B ), day 3 ( C ) and day 6 ( D ), stained with propidium iodide and the cell cycle distribution was analysed with flow cytometry. Fraction of cells present in G2/M-phase (G2/M), S-phase (S) and G1-phase (G1) are shown in purple, green and orange respectively. Fraction of apoptotic cells observed as a SubG1 peak is shown in black.
    Axio Imager A1 Microscopy System, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/axio imager a1 microscopy system/product/Carl Zeiss
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    Price from $9.99 to $1999.99
    axio imager a1 microscopy system - by Bioz Stars, 2020-07
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    99
    Carl Zeiss axio imager a1 digital camera
    Effects of anti-HER2 antibody BH1 and tumor necrosis factor-α on DNA synthesis and cell cycle in SK-BR-3 cells. ( A ) Effects on DNA synthesis were tested by 24-h BrdU incorporation assay. Cells were plated on coverslips in 12-well plates, treated as described in Figure 5 , and were exposed to BrdU during 24 h prior to indicated days. Nuclear BrdU was detected by indirect immunofluorescence assay. Nuclei were counterstained with DAPI, examined using Zeiss <t>Axio</t> Imager.A1 microscope. Representative photographs (3–5 frames per coverslip) were acquired. Percent BrdU incorporation was calculated after manual counting of BrdU + and BrdU - nuclei. * Denotes p-values ≤ 0.05 (BH1 vs. IgG1, BH1 + TNF-α vs. IgG1 + TNF-α). T-bars: SD. ( B - D ). Effects on cell cycle were analyzed by flow cytometry. SK-BR-3 cells were treated with 20 μg/ml BH1 or IgG isotype control antibody in the presence or absence of 1000 U/ml TNF-α. Cells were fixed on day 1 ( B ), day 3 ( C ) and day 6 ( D ), stained with propidium iodide and the cell cycle distribution was analysed with flow cytometry. Fraction of cells present in G2/M-phase (G2/M), S-phase (S) and G1-phase (G1) are shown in purple, green and orange respectively. Fraction of apoptotic cells observed as a SubG1 peak is shown in black.
    Axio Imager A1 Digital Camera, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Carl Zeiss axio imager a1 immunofluorescence microscope
    Effects of anti-HER2 antibody BH1 and tumor necrosis factor-α on DNA synthesis and cell cycle in SK-BR-3 cells. ( A ) Effects on DNA synthesis were tested by 24-h BrdU incorporation assay. Cells were plated on coverslips in 12-well plates, treated as described in Figure 5 , and were exposed to BrdU during 24 h prior to indicated days. Nuclear BrdU was detected by indirect immunofluorescence assay. Nuclei were counterstained with DAPI, examined using Zeiss <t>Axio</t> Imager.A1 microscope. Representative photographs (3–5 frames per coverslip) were acquired. Percent BrdU incorporation was calculated after manual counting of BrdU + and BrdU - nuclei. * Denotes p-values ≤ 0.05 (BH1 vs. IgG1, BH1 + TNF-α vs. IgG1 + TNF-α). T-bars: SD. ( B - D ). Effects on cell cycle were analyzed by flow cytometry. SK-BR-3 cells were treated with 20 μg/ml BH1 or IgG isotype control antibody in the presence or absence of 1000 U/ml TNF-α. Cells were fixed on day 1 ( B ), day 3 ( C ) and day 6 ( D ), stained with propidium iodide and the cell cycle distribution was analysed with flow cytometry. Fraction of cells present in G2/M-phase (G2/M), S-phase (S) and G1-phase (G1) are shown in purple, green and orange respectively. Fraction of apoptotic cells observed as a SubG1 peak is shown in black.
    Axio Imager A1 Immunofluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Carl Zeiss axio imager a1 compound microscope
    Effects of anti-HER2 antibody BH1 and tumor necrosis factor-α on DNA synthesis and cell cycle in SK-BR-3 cells. ( A ) Effects on DNA synthesis were tested by 24-h BrdU incorporation assay. Cells were plated on coverslips in 12-well plates, treated as described in Figure 5 , and were exposed to BrdU during 24 h prior to indicated days. Nuclear BrdU was detected by indirect immunofluorescence assay. Nuclei were counterstained with DAPI, examined using Zeiss <t>Axio</t> Imager.A1 microscope. Representative photographs (3–5 frames per coverslip) were acquired. Percent BrdU incorporation was calculated after manual counting of BrdU + and BrdU - nuclei. * Denotes p-values ≤ 0.05 (BH1 vs. IgG1, BH1 + TNF-α vs. IgG1 + TNF-α). T-bars: SD. ( B - D ). Effects on cell cycle were analyzed by flow cytometry. SK-BR-3 cells were treated with 20 μg/ml BH1 or IgG isotype control antibody in the presence or absence of 1000 U/ml TNF-α. Cells were fixed on day 1 ( B ), day 3 ( C ) and day 6 ( D ), stained with propidium iodide and the cell cycle distribution was analysed with flow cytometry. Fraction of cells present in G2/M-phase (G2/M), S-phase (S) and G1-phase (G1) are shown in purple, green and orange respectively. Fraction of apoptotic cells observed as a SubG1 peak is shown in black.
    Axio Imager A1 Compound Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 92/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Carl Zeiss model de axio imager a1 microscope
    Effects of anti-HER2 antibody BH1 and tumor necrosis factor-α on DNA synthesis and cell cycle in SK-BR-3 cells. ( A ) Effects on DNA synthesis were tested by 24-h BrdU incorporation assay. Cells were plated on coverslips in 12-well plates, treated as described in Figure 5 , and were exposed to BrdU during 24 h prior to indicated days. Nuclear BrdU was detected by indirect immunofluorescence assay. Nuclei were counterstained with DAPI, examined using Zeiss <t>Axio</t> Imager.A1 microscope. Representative photographs (3–5 frames per coverslip) were acquired. Percent BrdU incorporation was calculated after manual counting of BrdU + and BrdU - nuclei. * Denotes p-values ≤ 0.05 (BH1 vs. IgG1, BH1 + TNF-α vs. IgG1 + TNF-α). T-bars: SD. ( B - D ). Effects on cell cycle were analyzed by flow cytometry. SK-BR-3 cells were treated with 20 μg/ml BH1 or IgG isotype control antibody in the presence or absence of 1000 U/ml TNF-α. Cells were fixed on day 1 ( B ), day 3 ( C ) and day 6 ( D ), stained with propidium iodide and the cell cycle distribution was analysed with flow cytometry. Fraction of cells present in G2/M-phase (G2/M), S-phase (S) and G1-phase (G1) are shown in purple, green and orange respectively. Fraction of apoptotic cells observed as a SubG1 peak is shown in black.
    Model De Axio Imager A1 Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Effects of anti-HER2 antibody BH1 and tumor necrosis factor-α on DNA synthesis and cell cycle in SK-BR-3 cells. ( A ) Effects on DNA synthesis were tested by 24-h BrdU incorporation assay. Cells were plated on coverslips in 12-well plates, treated as described in Figure 5 , and were exposed to BrdU during 24 h prior to indicated days. Nuclear BrdU was detected by indirect immunofluorescence assay. Nuclei were counterstained with DAPI, examined using Zeiss Axio Imager.A1 microscope. Representative photographs (3–5 frames per coverslip) were acquired. Percent BrdU incorporation was calculated after manual counting of BrdU + and BrdU - nuclei. * Denotes p-values ≤ 0.05 (BH1 vs. IgG1, BH1 + TNF-α vs. IgG1 + TNF-α). T-bars: SD. ( B - D ). Effects on cell cycle were analyzed by flow cytometry. SK-BR-3 cells were treated with 20 μg/ml BH1 or IgG isotype control antibody in the presence or absence of 1000 U/ml TNF-α. Cells were fixed on day 1 ( B ), day 3 ( C ) and day 6 ( D ), stained with propidium iodide and the cell cycle distribution was analysed with flow cytometry. Fraction of cells present in G2/M-phase (G2/M), S-phase (S) and G1-phase (G1) are shown in purple, green and orange respectively. Fraction of apoptotic cells observed as a SubG1 peak is shown in black.

    Journal: BMC Cancer

    Article Title: Novel anti-HER2 monoclonal antibodies: synergy and antagonism with tumor necrosis factor-?

    doi: 10.1186/1471-2407-12-450

    Figure Lengend Snippet: Effects of anti-HER2 antibody BH1 and tumor necrosis factor-α on DNA synthesis and cell cycle in SK-BR-3 cells. ( A ) Effects on DNA synthesis were tested by 24-h BrdU incorporation assay. Cells were plated on coverslips in 12-well plates, treated as described in Figure 5 , and were exposed to BrdU during 24 h prior to indicated days. Nuclear BrdU was detected by indirect immunofluorescence assay. Nuclei were counterstained with DAPI, examined using Zeiss Axio Imager.A1 microscope. Representative photographs (3–5 frames per coverslip) were acquired. Percent BrdU incorporation was calculated after manual counting of BrdU + and BrdU - nuclei. * Denotes p-values ≤ 0.05 (BH1 vs. IgG1, BH1 + TNF-α vs. IgG1 + TNF-α). T-bars: SD. ( B - D ). Effects on cell cycle were analyzed by flow cytometry. SK-BR-3 cells were treated with 20 μg/ml BH1 or IgG isotype control antibody in the presence or absence of 1000 U/ml TNF-α. Cells were fixed on day 1 ( B ), day 3 ( C ) and day 6 ( D ), stained with propidium iodide and the cell cycle distribution was analysed with flow cytometry. Fraction of cells present in G2/M-phase (G2/M), S-phase (S) and G1-phase (G1) are shown in purple, green and orange respectively. Fraction of apoptotic cells observed as a SubG1 peak is shown in black.

    Article Snippet: Nuclei were counterstained with DAPI and cells were observed using Zeiss Axio Imager.A1 microscope (Jena, Germany).

    Techniques: DNA Synthesis, BrdU Incorporation Assay, Immunofluorescence, Microscopy, Flow Cytometry, Cytometry, Staining

    Strong binding of anti-HER2 antibodies to ERBB2 -amplified breast cancer cell lines. ERRB2 amplification was analyzed by FISH analysis. Cells were harvested at confluence, fixed and subjected to dual-color FISH using Texas Red-labeled ERBB2 (red) and FITC-labeled chromosome 17 α-satellite CEP17 (green) DNA probes (left panel). For generation of anti-HER2 monoclonal antibodies, mice were immunized with SK-BR-3 cells, followed by a recombinant protein composed of extracellular domain of HER2 fused to human IgG1 Fc domain (HER2 ECD). Antibodies were purified from conditioned hybridoma cell culture media by affinity chromatography. For immunofluorescence assay, cells were seeded on coverslips in 6-well plates, incubated 36 h in cell culture medium, fixed, and permeabilized. Fixed cells were incubated with anti-HER2 antibodies, and the primary antibody binding was detected using Alexa 488- conjugated anti-mouse IgG (BH1, BH2, BH5, BH6, BH7 panels; green). Nuclei were counterstained with DAPI (blue) and pictures were acquired using Zeiss Axio Imager.A1 microscope.

    Journal: BMC Cancer

    Article Title: Novel anti-HER2 monoclonal antibodies: synergy and antagonism with tumor necrosis factor-?

    doi: 10.1186/1471-2407-12-450

    Figure Lengend Snippet: Strong binding of anti-HER2 antibodies to ERBB2 -amplified breast cancer cell lines. ERRB2 amplification was analyzed by FISH analysis. Cells were harvested at confluence, fixed and subjected to dual-color FISH using Texas Red-labeled ERBB2 (red) and FITC-labeled chromosome 17 α-satellite CEP17 (green) DNA probes (left panel). For generation of anti-HER2 monoclonal antibodies, mice were immunized with SK-BR-3 cells, followed by a recombinant protein composed of extracellular domain of HER2 fused to human IgG1 Fc domain (HER2 ECD). Antibodies were purified from conditioned hybridoma cell culture media by affinity chromatography. For immunofluorescence assay, cells were seeded on coverslips in 6-well plates, incubated 36 h in cell culture medium, fixed, and permeabilized. Fixed cells were incubated with anti-HER2 antibodies, and the primary antibody binding was detected using Alexa 488- conjugated anti-mouse IgG (BH1, BH2, BH5, BH6, BH7 panels; green). Nuclei were counterstained with DAPI (blue) and pictures were acquired using Zeiss Axio Imager.A1 microscope.

    Article Snippet: Nuclei were counterstained with DAPI and cells were observed using Zeiss Axio Imager.A1 microscope (Jena, Germany).

    Techniques: Binding Assay, Amplification, Fluorescence In Situ Hybridization, Labeling, Mouse Assay, Recombinant, Purification, Cell Culture, Affinity Chromatography, Immunofluorescence, Incubation, Microscopy