Journal: Bioscience Reports

Article Title: Evaluation of subcutaneous infiltration of autologous platelet-rich plasma on skin-wound healing in dogs

doi: 10.1042/BSR20160503

Figure Lengend Snippet: Collagen bundles and collagen IA1 staining in control and PRP treated skin wounds Skin, dog. ( a ) Control wound and ( b ) PRP-treated wound at third week. Fibroblasts indicated by arrows, EP, epidermis; Ft, fibrous tissue (collagen) (Masson’s trichrome ×200). ( c ) Lesion score for collagen amount and organization recorded in second and third week. ( d ) Brown coloured collagen IA1 (arrows) in the control wound and ( e ) in the PRP wound at the second week. ( f ) Evaluation of the intensity of collagen IA1 in IHC stained sections at the second and third week. ( g ) Collagen IA1 staining in the control wound and ( h ) in the PRP wound at third week (avidin–biotin–peroxidase complex method, Mayer’s haematoxylin counterstain ×400).

Article Snippet: The immunohistochemical (IHC) staining was carried out using the primary antibody against COLIA1 (Novus Biologicals, Europe) prepared in rabbits and avidin–biotin–peroxidase complex method (Dako, LSAB +system-HRP, North America, Inc.).

Techniques: Staining, Immunohistochemistry, Avidin-Biotin Assay