avian myeloblastosis virus Search Results


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  • 99
    New England Biolabs avian myeloblastosis virus reverse transcriptase
    Avian Myeloblastosis Virus Reverse Transcriptase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 122 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher avian myeloblastosis virus reverse transcriptase
    Primer extension analysis for in vivo activity of Rv2966c. Primer extension reaction was carried out using avian <t>myeloblastosis</t> virus reverse transcriptase to monitor the in vivo methylation carried out by Rv2996c. Products were analyzed by running a
    Avian Myeloblastosis Virus Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 657 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega avian myeloblastosis virus reverse transcriptase
    Primer extension analysis of human brain RNA. Oligonucleotides GSP-6, PEXT1 and PEXT2 flanking the 5′ termini of the human LGA cDNA clone were 32 P end-labeled with [γ- 32 P]ATP and T4 polynucleotide kinase. Poly(A) + mRNA of human brain (approx. 0.5–1 µg) was mixed with the radioactive oligonucleotides and reverse-transcribed with avian <t>myeloblastosis</t> virus reverse transcriptase; the products from primer extension reactions were separated by electrophoresis on an 8% (w/v) denaturing polyacrylamide gel. Lanes are labeled with the name of the specific primer used in each case. The size of the single-stranded cDNA products (in bases) was estimated approximately by comparison with ФX174 HinfI DNA markers shown at the left in lane M. C+ lane: product amplified with a positive control of kanamycin. C- lanes: negative controls using PEXT2 with mRNA omitted. The black arrow indicates the specific GA products of approx. 140 bases obtained by reverse transcription with primer PEXT2. Top panel: scheme showing the approximate position of the three primers with regard to the TSS (+1) and the two first ATG codons of human LGA transcript.
    Avian Myeloblastosis Virus Reverse Transcriptase, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 4113 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Boehringer Mannheim avian myeloblastosis virus reverse transcriptase
    Transgenic mRNA and protein expression. ( A ) Design of primers to detect spliced hybrid GAD-HGH RNA (see text). ( B ) Reverse transcription–PCR analysis of tissues from tg(+) mice ( + ) and non-tg(+) mice (−) mice, in the presence (RT+) or absence (RT−) of avian <t>myeloblastosis</t> virus reverse transcriptase (AMV-RT) by using transgene-specific primers ( Upper ) and hypoxanthine-guanine phosphoribosyltransferase-specific primers ( Lower ). M, 100-bp molecular marker (GIBCO/BRL); B, brain; K, kidney; L, liver; T, thymus; H, heart; I, islets. The origin of the different bands is discussed in the text. Tissue lysates from adult ( C and D ) or day 2 ( E ) tg(+) mice ( + ) and non-tg(+) mice (−) were immunoprecipitated with Stiff Man Syndrome patient serum and Western blotted with mAb mN65 against GAD65 ( C and E ). The membrane in C was stripped and reprobed with GAD65-specific rabbit polyclonal antisera, 7673 ( D ). Sizes of proteins were estimated from coelectrophoresed, prestained markers (not shown).
    Avian Myeloblastosis Virus Reverse Transcriptase, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 92/100, based on 339 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega avian myeloblastosis virus rt
    Transgenic mRNA and protein expression. ( A ) Design of primers to detect spliced hybrid GAD-HGH RNA (see text). ( B ) Reverse transcription–PCR analysis of tissues from tg(+) mice ( + ) and non-tg(+) mice (−) mice, in the presence (RT+) or absence (RT−) of avian <t>myeloblastosis</t> virus reverse transcriptase (AMV-RT) by using transgene-specific primers ( Upper ) and hypoxanthine-guanine phosphoribosyltransferase-specific primers ( Lower ). M, 100-bp molecular marker (GIBCO/BRL); B, brain; K, kidney; L, liver; T, thymus; H, heart; I, islets. The origin of the different bands is discussed in the text. Tissue lysates from adult ( C and D ) or day 2 ( E ) tg(+) mice ( + ) and non-tg(+) mice (−) were immunoprecipitated with Stiff Man Syndrome patient serum and Western blotted with mAb mN65 against GAD65 ( C and E ). The membrane in C was stripped and reprobed with GAD65-specific rabbit polyclonal antisera, 7673 ( D ). Sizes of proteins were estimated from coelectrophoresed, prestained markers (not shown).
    Avian Myeloblastosis Virus Rt, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 397 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare avian myeloblastosis virus reverse transcriptase
    Transgenic mRNA and protein expression. ( A ) Design of primers to detect spliced hybrid GAD-HGH RNA (see text). ( B ) Reverse transcription–PCR analysis of tissues from tg(+) mice ( + ) and non-tg(+) mice (−) mice, in the presence (RT+) or absence (RT−) of avian <t>myeloblastosis</t> virus reverse transcriptase (AMV-RT) by using transgene-specific primers ( Upper ) and hypoxanthine-guanine phosphoribosyltransferase-specific primers ( Lower ). M, 100-bp molecular marker (GIBCO/BRL); B, brain; K, kidney; L, liver; T, thymus; H, heart; I, islets. The origin of the different bands is discussed in the text. Tissue lysates from adult ( C and D ) or day 2 ( E ) tg(+) mice ( + ) and non-tg(+) mice (−) were immunoprecipitated with Stiff Man Syndrome patient serum and Western blotted with mAb mN65 against GAD65 ( C and E ). The membrane in C was stripped and reprobed with GAD65-specific rabbit polyclonal antisera, 7673 ( D ). Sizes of proteins were estimated from coelectrophoresed, prestained markers (not shown).
    Avian Myeloblastosis Virus Reverse Transcriptase, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 306 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Seikagaku avian myeloblastosis virus reverse transcriptase
    Transgenic mRNA and protein expression. ( A ) Design of primers to detect spliced hybrid GAD-HGH RNA (see text). ( B ) Reverse transcription–PCR analysis of tissues from tg(+) mice ( + ) and non-tg(+) mice (−) mice, in the presence (RT+) or absence (RT−) of avian <t>myeloblastosis</t> virus reverse transcriptase (AMV-RT) by using transgene-specific primers ( Upper ) and hypoxanthine-guanine phosphoribosyltransferase-specific primers ( Lower ). M, 100-bp molecular marker (GIBCO/BRL); B, brain; K, kidney; L, liver; T, thymus; H, heart; I, islets. The origin of the different bands is discussed in the text. Tissue lysates from adult ( C and D ) or day 2 ( E ) tg(+) mice ( + ) and non-tg(+) mice (−) were immunoprecipitated with Stiff Man Syndrome patient serum and Western blotted with mAb mN65 against GAD65 ( C and E ). The membrane in C was stripped and reprobed with GAD65-specific rabbit polyclonal antisera, 7673 ( D ). Sizes of proteins were estimated from coelectrophoresed, prestained markers (not shown).
    Avian Myeloblastosis Virus Reverse Transcriptase, supplied by Seikagaku, used in various techniques. Bioz Stars score: 92/100, based on 147 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene avian myeloblastosis virus reverse transcriptase
    Transgenic mRNA and protein expression. ( A ) Design of primers to detect spliced hybrid GAD-HGH RNA (see text). ( B ) Reverse transcription–PCR analysis of tissues from tg(+) mice ( + ) and non-tg(+) mice (−) mice, in the presence (RT+) or absence (RT−) of avian <t>myeloblastosis</t> virus reverse transcriptase (AMV-RT) by using transgene-specific primers ( Upper ) and hypoxanthine-guanine phosphoribosyltransferase-specific primers ( Lower ). M, 100-bp molecular marker (GIBCO/BRL); B, brain; K, kidney; L, liver; T, thymus; H, heart; I, islets. The origin of the different bands is discussed in the text. Tissue lysates from adult ( C and D ) or day 2 ( E ) tg(+) mice ( + ) and non-tg(+) mice (−) were immunoprecipitated with Stiff Man Syndrome patient serum and Western blotted with mAb mN65 against GAD65 ( C and E ). The membrane in C was stripped and reprobed with GAD65-specific rabbit polyclonal antisera, 7673 ( D ). Sizes of proteins were estimated from coelectrophoresed, prestained markers (not shown).
    Avian Myeloblastosis Virus Reverse Transcriptase, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa avian myeloblastosis virus amv reverse transcriptase xl
    Transgenic mRNA and protein expression. ( A ) Design of primers to detect spliced hybrid GAD-HGH RNA (see text). ( B ) Reverse transcription–PCR analysis of tissues from tg(+) mice ( + ) and non-tg(+) mice (−) mice, in the presence (RT+) or absence (RT−) of avian <t>myeloblastosis</t> virus reverse transcriptase (AMV-RT) by using transgene-specific primers ( Upper ) and hypoxanthine-guanine phosphoribosyltransferase-specific primers ( Lower ). M, 100-bp molecular marker (GIBCO/BRL); B, brain; K, kidney; L, liver; T, thymus; H, heart; I, islets. The origin of the different bands is discussed in the text. Tissue lysates from adult ( C and D ) or day 2 ( E ) tg(+) mice ( + ) and non-tg(+) mice (−) were immunoprecipitated with Stiff Man Syndrome patient serum and Western blotted with mAb mN65 against GAD65 ( C and E ). The membrane in C was stripped and reprobed with GAD65-specific rabbit polyclonal antisera, 7673 ( D ). Sizes of proteins were estimated from coelectrophoresed, prestained markers (not shown).
    Avian Myeloblastosis Virus Amv Reverse Transcriptase Xl, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega avian myeloblastosis virus reverse transcription
    Transgenic mRNA and protein expression. ( A ) Design of primers to detect spliced hybrid GAD-HGH RNA (see text). ( B ) Reverse transcription–PCR analysis of tissues from tg(+) mice ( + ) and non-tg(+) mice (−) mice, in the presence (RT+) or absence (RT−) of avian <t>myeloblastosis</t> virus reverse transcriptase (AMV-RT) by using transgene-specific primers ( Upper ) and hypoxanthine-guanine phosphoribosyltransferase-specific primers ( Lower ). M, 100-bp molecular marker (GIBCO/BRL); B, brain; K, kidney; L, liver; T, thymus; H, heart; I, islets. The origin of the different bands is discussed in the text. Tissue lysates from adult ( C and D ) or day 2 ( E ) tg(+) mice ( + ) and non-tg(+) mice (−) were immunoprecipitated with Stiff Man Syndrome patient serum and Western blotted with mAb mN65 against GAD65 ( C and E ). The membrane in C was stripped and reprobed with GAD65-specific rabbit polyclonal antisera, 7673 ( D ). Sizes of proteins were estimated from coelectrophoresed, prestained markers (not shown).
    Avian Myeloblastosis Virus Reverse Transcription, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega avian myeloblastosis virus reverse transcription system
    Transgenic mRNA and protein expression. ( A ) Design of primers to detect spliced hybrid GAD-HGH RNA (see text). ( B ) Reverse transcription–PCR analysis of tissues from tg(+) mice ( + ) and non-tg(+) mice (−) mice, in the presence (RT+) or absence (RT−) of avian <t>myeloblastosis</t> virus reverse transcriptase (AMV-RT) by using transgene-specific primers ( Upper ) and hypoxanthine-guanine phosphoribosyltransferase-specific primers ( Lower ). M, 100-bp molecular marker (GIBCO/BRL); B, brain; K, kidney; L, liver; T, thymus; H, heart; I, islets. The origin of the different bands is discussed in the text. Tissue lysates from adult ( C and D ) or day 2 ( E ) tg(+) mice ( + ) and non-tg(+) mice (−) were immunoprecipitated with Stiff Man Syndrome patient serum and Western blotted with mAb mN65 against GAD65 ( C and E ). The membrane in C was stripped and reprobed with GAD65-specific rabbit polyclonal antisera, 7673 ( D ). Sizes of proteins were estimated from coelectrophoresed, prestained markers (not shown).
    Avian Myeloblastosis Virus Reverse Transcription System, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 65 article reviews
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    Image Search Results


    Primer extension analysis for in vivo activity of Rv2966c. Primer extension reaction was carried out using avian myeloblastosis virus reverse transcriptase to monitor the in vivo methylation carried out by Rv2996c. Products were analyzed by running a

    Journal: The Journal of Biological Chemistry

    Article Title: Structural and Functional Characterization of Rv2966c Protein Reveals an RsmD-like Methyltransferase from Mycobacterium tuberculosis and the Role of Its N-terminal Domain in Target Recognition *

    doi: 10.1074/jbc.M110.200428

    Figure Lengend Snippet: Primer extension analysis for in vivo activity of Rv2966c. Primer extension reaction was carried out using avian myeloblastosis virus reverse transcriptase to monitor the in vivo methylation carried out by Rv2996c. Products were analyzed by running a

    Article Snippet: Primer extension was carried out using avian myeloblastosis virus reverse transcriptase (Fermentas) as per the manufacturer's protocol.

    Techniques: In Vivo, Activity Assay, Methylation

    Primer extension analysis of human brain RNA. Oligonucleotides GSP-6, PEXT1 and PEXT2 flanking the 5′ termini of the human LGA cDNA clone were 32 P end-labeled with [γ- 32 P]ATP and T4 polynucleotide kinase. Poly(A) + mRNA of human brain (approx. 0.5–1 µg) was mixed with the radioactive oligonucleotides and reverse-transcribed with avian myeloblastosis virus reverse transcriptase; the products from primer extension reactions were separated by electrophoresis on an 8% (w/v) denaturing polyacrylamide gel. Lanes are labeled with the name of the specific primer used in each case. The size of the single-stranded cDNA products (in bases) was estimated approximately by comparison with ФX174 HinfI DNA markers shown at the left in lane M. C+ lane: product amplified with a positive control of kanamycin. C- lanes: negative controls using PEXT2 with mRNA omitted. The black arrow indicates the specific GA products of approx. 140 bases obtained by reverse transcription with primer PEXT2. Top panel: scheme showing the approximate position of the three primers with regard to the TSS (+1) and the two first ATG codons of human LGA transcript.

    Journal: PLoS ONE

    Article Title: Mammalian Glutaminase Gls2 Gene Encodes Two Functional Alternative Transcripts by a Surrogate Promoter Usage Mechanism

    doi: 10.1371/journal.pone.0038380

    Figure Lengend Snippet: Primer extension analysis of human brain RNA. Oligonucleotides GSP-6, PEXT1 and PEXT2 flanking the 5′ termini of the human LGA cDNA clone were 32 P end-labeled with [γ- 32 P]ATP and T4 polynucleotide kinase. Poly(A) + mRNA of human brain (approx. 0.5–1 µg) was mixed with the radioactive oligonucleotides and reverse-transcribed with avian myeloblastosis virus reverse transcriptase; the products from primer extension reactions were separated by electrophoresis on an 8% (w/v) denaturing polyacrylamide gel. Lanes are labeled with the name of the specific primer used in each case. The size of the single-stranded cDNA products (in bases) was estimated approximately by comparison with ФX174 HinfI DNA markers shown at the left in lane M. C+ lane: product amplified with a positive control of kanamycin. C- lanes: negative controls using PEXT2 with mRNA omitted. The black arrow indicates the specific GA products of approx. 140 bases obtained by reverse transcription with primer PEXT2. Top panel: scheme showing the approximate position of the three primers with regard to the TSS (+1) and the two first ATG codons of human LGA transcript.

    Article Snippet: 0.5–1 µg) was mixed with the radioactive oligonucleotides and reverse transcribed with avian myeloblastosis virus reverse transcriptase, with the use of the protocol provided by the manufacturer (Primer Extension Analysis kit; Promega).

    Techniques: Labeling, Electrophoresis, Amplification, Positive Control

    Transcript analysis of the brnQ gene. (A) Northern blot analysis of ORF442. Ten micrograms of an RNA sample was separated electrophoretically and transferred by Northern blotting onto a membrane. The blot was probed with a radiolabeled 2.5-kb DNA fragment encompassing brnQ . The sizes of the ribosomal RNAs are marked with arrowheads, and the BrnQ product is indicated by an arrow. (B) Mapping of the 5′ end of the brnQ transcript by primer extension analysis. Total RNA from the parent strain was hybridized with an oligonucleotide complementary to the mRNA of the brnQ locus and extended by avian myeloblastosis virus reverse transcriptase (lane P). Lanes T, G, C, and A correspond to a dideoxy sequencing reaction performed with the same primer. The sequence encompassing the initiation start site (marked by an arrowhead) is enlarged.

    Journal: Applied and Environmental Microbiology

    Article Title: Cloning and Nucleotide Sequencing of a Staphylococcus aureus Gene Encoding a Branched-Chain-Amino-Acid Transporter

    doi:

    Figure Lengend Snippet: Transcript analysis of the brnQ gene. (A) Northern blot analysis of ORF442. Ten micrograms of an RNA sample was separated electrophoretically and transferred by Northern blotting onto a membrane. The blot was probed with a radiolabeled 2.5-kb DNA fragment encompassing brnQ . The sizes of the ribosomal RNAs are marked with arrowheads, and the BrnQ product is indicated by an arrow. (B) Mapping of the 5′ end of the brnQ transcript by primer extension analysis. Total RNA from the parent strain was hybridized with an oligonucleotide complementary to the mRNA of the brnQ locus and extended by avian myeloblastosis virus reverse transcriptase (lane P). Lanes T, G, C, and A correspond to a dideoxy sequencing reaction performed with the same primer. The sequence encompassing the initiation start site (marked by an arrowhead) is enlarged.

    Article Snippet: To define the transcription unit more precisely, the 5′ end of the transcript was mapped with the avian myeloblastosis virus reverse transcriptase system (Promega).

    Techniques: Northern Blot, Sequencing

    Transgenic mRNA and protein expression. ( A ) Design of primers to detect spliced hybrid GAD-HGH RNA (see text). ( B ) Reverse transcription–PCR analysis of tissues from tg(+) mice ( + ) and non-tg(+) mice (−) mice, in the presence (RT+) or absence (RT−) of avian myeloblastosis virus reverse transcriptase (AMV-RT) by using transgene-specific primers ( Upper ) and hypoxanthine-guanine phosphoribosyltransferase-specific primers ( Lower ). M, 100-bp molecular marker (GIBCO/BRL); B, brain; K, kidney; L, liver; T, thymus; H, heart; I, islets. The origin of the different bands is discussed in the text. Tissue lysates from adult ( C and D ) or day 2 ( E ) tg(+) mice ( + ) and non-tg(+) mice (−) were immunoprecipitated with Stiff Man Syndrome patient serum and Western blotted with mAb mN65 against GAD65 ( C and E ). The membrane in C was stripped and reprobed with GAD65-specific rabbit polyclonal antisera, 7673 ( D ). Sizes of proteins were estimated from coelectrophoresed, prestained markers (not shown).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Widespread expression of an autoantigen-GAD65 transgene does not tolerize non-obese diabetic mice and can exacerbate disease

    doi:

    Figure Lengend Snippet: Transgenic mRNA and protein expression. ( A ) Design of primers to detect spliced hybrid GAD-HGH RNA (see text). ( B ) Reverse transcription–PCR analysis of tissues from tg(+) mice ( + ) and non-tg(+) mice (−) mice, in the presence (RT+) or absence (RT−) of avian myeloblastosis virus reverse transcriptase (AMV-RT) by using transgene-specific primers ( Upper ) and hypoxanthine-guanine phosphoribosyltransferase-specific primers ( Lower ). M, 100-bp molecular marker (GIBCO/BRL); B, brain; K, kidney; L, liver; T, thymus; H, heart; I, islets. The origin of the different bands is discussed in the text. Tissue lysates from adult ( C and D ) or day 2 ( E ) tg(+) mice ( + ) and non-tg(+) mice (−) were immunoprecipitated with Stiff Man Syndrome patient serum and Western blotted with mAb mN65 against GAD65 ( C and E ). The membrane in C was stripped and reprobed with GAD65-specific rabbit polyclonal antisera, 7673 ( D ). Sizes of proteins were estimated from coelectrophoresed, prestained markers (not shown).

    Article Snippet: Five micrograms of tissue RNA extracted with guanidium isothiocyanate/phenol (RNAzol, Cinna/Biotecx, Houston, TX) were primed with random hexamers, reverse-transcribed with avian myeloblastosis virus reverse transcriptase (Boehringer Mannheim), and equal amounts (quantified by spectrophotometry) amplified by PCR.

    Techniques: Transgenic Assay, Expressing, Polymerase Chain Reaction, Mouse Assay, Marker, Immunoprecipitation, Western Blot