atpase Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Millipore anti na k atpase antibody
    Decreased K ATP channel subunit expression in ankyrin-B +/− hearts. Detergent-soluble ankyrin-B +/− heart lysates expressed significantly less ankyrin-B ( A ), Kir6.2 ( B ), SUR1 ( C ), SUR2A ( D ), <t>Na/K-ATPase</t> ( H ), and NCX1 ( I ). There were no significant differences in the expression of ankyrin-G ( E ), Kir6.1 ( F ), or NHERF1 (loading control; G ). J , quantitative data demonstrating a significant decrease in the expression of ankyrin-B, NCX1, Na/K-ATPase, and K ATP channel proteins in ankyrin-B +/− compared with wild-type detergent-soluble mouse heart lysates. Expression of proteins was normalized to wild-type expression. IB , immunoblot. *, p
    Anti Na K Atpase Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti na k atpase antibody/product/Millipore
    Average 95 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    anti na k atpase antibody - by Bioz Stars, 2020-02
    95/100 stars
      Buy from Supplier

    78
    Agrisera vacuolor adenosine triphosphatase v atpase antibody
    Decreased K ATP channel subunit expression in ankyrin-B +/− hearts. Detergent-soluble ankyrin-B +/− heart lysates expressed significantly less ankyrin-B ( A ), Kir6.2 ( B ), SUR1 ( C ), SUR2A ( D ), <t>Na/K-ATPase</t> ( H ), and NCX1 ( I ). There were no significant differences in the expression of ankyrin-G ( E ), Kir6.1 ( F ), or NHERF1 (loading control; G ). J , quantitative data demonstrating a significant decrease in the expression of ankyrin-B, NCX1, Na/K-ATPase, and K ATP channel proteins in ankyrin-B +/− compared with wild-type detergent-soluble mouse heart lysates. Expression of proteins was normalized to wild-type expression. IB , immunoblot. *, p
    Vacuolor Adenosine Triphosphatase V Atpase Antibody, supplied by Agrisera, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vacuolor adenosine triphosphatase v atpase antibody/product/Agrisera
    Average 78 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    vacuolor adenosine triphosphatase v atpase antibody - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    90
    Cell Signaling Technology Inc na k atpase
    Muscles of LAMA2 MD patients and dy W /dy W mice contain high amounts of laminin-α4 and show deficits in BM ( A ) Representative immunofluorescence images (magnified images in insets) of human muscle biopsy cross sections stained for laminin-α4 and laminin-β1γ1. n = 3 LAMA2 MD patients or healthy controls. ( B ) Western blot analysis and quantification of laminin chains in human muscle biopsies. α-Actinin was used as loading control. n = 4 controls, n = 3 LAMA2 MD patients. ( C ) Representative immunofluorescence images (magnified images in insets) of triceps muscle from 8-week-old mice stained for laminin-α4 and laminin-β1γ1. n = 4 mice per genotype. ( D ) Western blot analysis and quantification of laminin-α4 (left graph), laminin-β1γ1 (middle graph), and Lama4 transcripts (right graph) from triceps muscle of 8-week-old mice. n = 3 mice per genotype (Western blot) and n = 4 mice per genotype (quantitative reverse transcription polymerase chain reaction). ( E ) Schematic of subcellular fractionation. ( F ) Western blot analysis of S1 to S4 of a muscle biopsy from a control patient. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and Na + /K + <t>-ATPase</t> (sodium-potassium adenosine triphosphatase) were used as markers for cytosolic and membrane proteins, respectively. ( G ) Western blot analysis of S3 and S4 fractions from control and LAMA2 MD patient muscle biopsies probed for the indicated proteins. Right: Ratio of the laminin-β1γ1 intensity in S3 and S4 fraction. n = 4 healthy controls, n = 3 LAMA2 MD patients. Data are means ± SEM. * P
    Na K Atpase, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 441 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/na k atpase/product/Cell Signaling Technology Inc
    Average 90 stars, based on 441 article reviews
    Price from $9.99 to $1999.99
    na k atpase - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

    79
    SAS institute atpases
    Muscles of LAMA2 MD patients and dy W /dy W mice contain high amounts of laminin-α4 and show deficits in BM ( A ) Representative immunofluorescence images (magnified images in insets) of human muscle biopsy cross sections stained for laminin-α4 and laminin-β1γ1. n = 3 LAMA2 MD patients or healthy controls. ( B ) Western blot analysis and quantification of laminin chains in human muscle biopsies. α-Actinin was used as loading control. n = 4 controls, n = 3 LAMA2 MD patients. ( C ) Representative immunofluorescence images (magnified images in insets) of triceps muscle from 8-week-old mice stained for laminin-α4 and laminin-β1γ1. n = 4 mice per genotype. ( D ) Western blot analysis and quantification of laminin-α4 (left graph), laminin-β1γ1 (middle graph), and Lama4 transcripts (right graph) from triceps muscle of 8-week-old mice. n = 3 mice per genotype (Western blot) and n = 4 mice per genotype (quantitative reverse transcription polymerase chain reaction). ( E ) Schematic of subcellular fractionation. ( F ) Western blot analysis of S1 to S4 of a muscle biopsy from a control patient. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and Na + /K + <t>-ATPase</t> (sodium-potassium adenosine triphosphatase) were used as markers for cytosolic and membrane proteins, respectively. ( G ) Western blot analysis of S3 and S4 fractions from control and LAMA2 MD patient muscle biopsies probed for the indicated proteins. Right: Ratio of the laminin-β1γ1 intensity in S3 and S4 fraction. n = 4 healthy controls, n = 3 LAMA2 MD patients. Data are means ± SEM. * P
    Atpases, supplied by SAS institute, used in various techniques. Bioz Stars score: 79/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atpases/product/SAS institute
    Average 79 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    atpases - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    79
    rdi research diagnostics atpases
    Muscles of LAMA2 MD patients and dy W /dy W mice contain high amounts of laminin-α4 and show deficits in BM ( A ) Representative immunofluorescence images (magnified images in insets) of human muscle biopsy cross sections stained for laminin-α4 and laminin-β1γ1. n = 3 LAMA2 MD patients or healthy controls. ( B ) Western blot analysis and quantification of laminin chains in human muscle biopsies. α-Actinin was used as loading control. n = 4 controls, n = 3 LAMA2 MD patients. ( C ) Representative immunofluorescence images (magnified images in insets) of triceps muscle from 8-week-old mice stained for laminin-α4 and laminin-β1γ1. n = 4 mice per genotype. ( D ) Western blot analysis and quantification of laminin-α4 (left graph), laminin-β1γ1 (middle graph), and Lama4 transcripts (right graph) from triceps muscle of 8-week-old mice. n = 3 mice per genotype (Western blot) and n = 4 mice per genotype (quantitative reverse transcription polymerase chain reaction). ( E ) Schematic of subcellular fractionation. ( F ) Western blot analysis of S1 to S4 of a muscle biopsy from a control patient. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and Na + /K + <t>-ATPase</t> (sodium-potassium adenosine triphosphatase) were used as markers for cytosolic and membrane proteins, respectively. ( G ) Western blot analysis of S3 and S4 fractions from control and LAMA2 MD patient muscle biopsies probed for the indicated proteins. Right: Ratio of the laminin-β1γ1 intensity in S3 and S4 fraction. n = 4 healthy controls, n = 3 LAMA2 MD patients. Data are means ± SEM. * P
    Atpases, supplied by rdi research diagnostics, used in various techniques. Bioz Stars score: 79/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atpases/product/rdi research diagnostics
    Average 79 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    atpases - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    79
    Cytoskeleton Inc atpases
    Muscles of LAMA2 MD patients and dy W /dy W mice contain high amounts of laminin-α4 and show deficits in BM ( A ) Representative immunofluorescence images (magnified images in insets) of human muscle biopsy cross sections stained for laminin-α4 and laminin-β1γ1. n = 3 LAMA2 MD patients or healthy controls. ( B ) Western blot analysis and quantification of laminin chains in human muscle biopsies. α-Actinin was used as loading control. n = 4 controls, n = 3 LAMA2 MD patients. ( C ) Representative immunofluorescence images (magnified images in insets) of triceps muscle from 8-week-old mice stained for laminin-α4 and laminin-β1γ1. n = 4 mice per genotype. ( D ) Western blot analysis and quantification of laminin-α4 (left graph), laminin-β1γ1 (middle graph), and Lama4 transcripts (right graph) from triceps muscle of 8-week-old mice. n = 3 mice per genotype (Western blot) and n = 4 mice per genotype (quantitative reverse transcription polymerase chain reaction). ( E ) Schematic of subcellular fractionation. ( F ) Western blot analysis of S1 to S4 of a muscle biopsy from a control patient. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and Na + /K + <t>-ATPase</t> (sodium-potassium adenosine triphosphatase) were used as markers for cytosolic and membrane proteins, respectively. ( G ) Western blot analysis of S3 and S4 fractions from control and LAMA2 MD patient muscle biopsies probed for the indicated proteins. Right: Ratio of the laminin-β1γ1 intensity in S3 and S4 fraction. n = 4 healthy controls, n = 3 LAMA2 MD patients. Data are means ± SEM. * P
    Atpases, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 79/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atpases/product/Cytoskeleton Inc
    Average 79 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    atpases - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    88
    Developmental Studies Hybridoma Bank k atpase
    The immunoblots and relative intensities of Prdx6 in membrane-fraction proteins of livers of the fresh water (FW) and seawater (SW) milkfish acclimated to 28°C (white bar) and 18°C (stripe bar) for 1 week, respectively . In the representative immunoblot, single immunoreactive bands at 100 and 25 kDa represent Na + , K + <t>-ATPase</t> <t>(NKA),</t> and Prdx6, respectively. The NKA was used as the loading control of the membrane fraction. Different letters (a and b) indicate significant differences between the 28° and 18°C group, and (x and y) indicate significant differences between the FW and SW group. Values are means ± SEM, n = 6. The Student's t -test pairwise comparison was used following two-way ANOVA, P
    K Atpase, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 88/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k atpase/product/Developmental Studies Hybridoma Bank
    Average 88 stars, based on 51 article reviews
    Price from $9.99 to $1999.99
    k atpase - by Bioz Stars, 2020-02
    88/100 stars
      Buy from Supplier

    93
    Millipore k atpase
    The immunoblots and relative intensities of Prdx6 in membrane-fraction proteins of livers of the fresh water (FW) and seawater (SW) milkfish acclimated to 28°C (white bar) and 18°C (stripe bar) for 1 week, respectively . In the representative immunoblot, single immunoreactive bands at 100 and 25 kDa represent Na + , K + <t>-ATPase</t> <t>(NKA),</t> and Prdx6, respectively. The NKA was used as the loading control of the membrane fraction. Different letters (a and b) indicate significant differences between the 28° and 18°C group, and (x and y) indicate significant differences between the FW and SW group. Values are means ± SEM, n = 6. The Student's t -test pairwise comparison was used following two-way ANOVA, P
    K Atpase, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k atpase/product/Millipore
    Average 93 stars, based on 83 article reviews
    Price from $9.99 to $1999.99
    k atpase - by Bioz Stars, 2020-02
    93/100 stars
      Buy from Supplier

    79
    Seca Inc atpase seca
    The immunoblots and relative intensities of Prdx6 in membrane-fraction proteins of livers of the fresh water (FW) and seawater (SW) milkfish acclimated to 28°C (white bar) and 18°C (stripe bar) for 1 week, respectively . In the representative immunoblot, single immunoreactive bands at 100 and 25 kDa represent Na + , K + <t>-ATPase</t> <t>(NKA),</t> and Prdx6, respectively. The NKA was used as the loading control of the membrane fraction. Different letters (a and b) indicate significant differences between the 28° and 18°C group, and (x and y) indicate significant differences between the FW and SW group. Values are means ± SEM, n = 6. The Student's t -test pairwise comparison was used following two-way ANOVA, P
    Atpase Seca, supplied by Seca Inc, used in various techniques. Bioz Stars score: 79/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atpase seca/product/Seca Inc
    Average 79 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    atpase seca - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    77
    Tocris atpase inhibitor
    The immunoblots and relative intensities of Prdx6 in membrane-fraction proteins of livers of the fresh water (FW) and seawater (SW) milkfish acclimated to 28°C (white bar) and 18°C (stripe bar) for 1 week, respectively . In the representative immunoblot, single immunoreactive bands at 100 and 25 kDa represent Na + , K + <t>-ATPase</t> <t>(NKA),</t> and Prdx6, respectively. The NKA was used as the loading control of the membrane fraction. Different letters (a and b) indicate significant differences between the 28° and 18°C group, and (x and y) indicate significant differences between the FW and SW group. Values are means ± SEM, n = 6. The Student's t -test pairwise comparison was used following two-way ANOVA, P
    Atpase Inhibitor, supplied by Tocris, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atpase inhibitor/product/Tocris
    Average 77 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    atpase inhibitor - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    97
    Agrisera v atpase
    Electrophoretic polypeptide pattern and Western blot analysis of tonoplast proteins from the potato cultivars Desiree and Mozart. Tonoplast vesicles were isolated from leaves of plants grown in the absence and presence of NaCl as indicated in the figure by 0 and 60 mM, respectively. (A,B) Coomassie blue stained gel of tonoplast proteins from Desiree and Mozart, respectively. Lane M represents the protein marker. Western blots (C–J) were scanned into gray scale and the optical density was quantified by Scion Image. Blots were probed with <t>anti-V-PPase</t> (C–F) and <t>anti-V-ATPase</t> (A-subunit) (G–J) of mung bean. Panels (C,G,D,H) show the effect of salt treatment on the amount of V-H + -ATPase and the V-H + -PPase in both cultivars. Panels (E,I,F,J) show the effect of the cultivar on the amount of V-H + -ATPase and the V-H + -PPase in both salt treatments. The molecular masses of the immunostained polypeptides are indicated (arrows). Values represent mean ± SEM of four independent replicates. Asterisks indicate a statistically significant treatment effect (Student’s t -test; P
    V Atpase, supplied by Agrisera, used in various techniques. Bioz Stars score: 97/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/v atpase/product/Agrisera
    Average 97 stars, based on 32 article reviews
    Price from $9.99 to $1999.99
    v atpase - by Bioz Stars, 2020-02
    97/100 stars
      Buy from Supplier

    76
    Becton Dickinson mitochondrial atpase
    Electrophoretic polypeptide pattern and Western blot analysis of tonoplast proteins from the potato cultivars Desiree and Mozart. Tonoplast vesicles were isolated from leaves of plants grown in the absence and presence of NaCl as indicated in the figure by 0 and 60 mM, respectively. (A,B) Coomassie blue stained gel of tonoplast proteins from Desiree and Mozart, respectively. Lane M represents the protein marker. Western blots (C–J) were scanned into gray scale and the optical density was quantified by Scion Image. Blots were probed with <t>anti-V-PPase</t> (C–F) and <t>anti-V-ATPase</t> (A-subunit) (G–J) of mung bean. Panels (C,G,D,H) show the effect of salt treatment on the amount of V-H + -ATPase and the V-H + -PPase in both cultivars. Panels (E,I,F,J) show the effect of the cultivar on the amount of V-H + -ATPase and the V-H + -PPase in both salt treatments. The molecular masses of the immunostained polypeptides are indicated (arrows). Values represent mean ± SEM of four independent replicates. Asterisks indicate a statistically significant treatment effect (Student’s t -test; P
    Mitochondrial Atpase, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 76/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mitochondrial atpase/product/Becton Dickinson
    Average 76 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    mitochondrial atpase - by Bioz Stars, 2020-02
    76/100 stars
      Buy from Supplier

    87
    MBL International k atpase
    Electrophoretic polypeptide pattern and Western blot analysis of tonoplast proteins from the potato cultivars Desiree and Mozart. Tonoplast vesicles were isolated from leaves of plants grown in the absence and presence of NaCl as indicated in the figure by 0 and 60 mM, respectively. (A,B) Coomassie blue stained gel of tonoplast proteins from Desiree and Mozart, respectively. Lane M represents the protein marker. Western blots (C–J) were scanned into gray scale and the optical density was quantified by Scion Image. Blots were probed with <t>anti-V-PPase</t> (C–F) and <t>anti-V-ATPase</t> (A-subunit) (G–J) of mung bean. Panels (C,G,D,H) show the effect of salt treatment on the amount of V-H + -ATPase and the V-H + -PPase in both cultivars. Panels (E,I,F,J) show the effect of the cultivar on the amount of V-H + -ATPase and the V-H + -PPase in both salt treatments. The molecular masses of the immunostained polypeptides are indicated (arrows). Values represent mean ± SEM of four independent replicates. Asterisks indicate a statistically significant treatment effect (Student’s t -test; P
    K Atpase, supplied by MBL International, used in various techniques. Bioz Stars score: 87/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k atpase/product/MBL International
    Average 87 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    k atpase - by Bioz Stars, 2020-02
    87/100 stars
      Buy from Supplier

    77
    Millipore control atpase
    Electrophoretic polypeptide pattern and Western blot analysis of tonoplast proteins from the potato cultivars Desiree and Mozart. Tonoplast vesicles were isolated from leaves of plants grown in the absence and presence of NaCl as indicated in the figure by 0 and 60 mM, respectively. (A,B) Coomassie blue stained gel of tonoplast proteins from Desiree and Mozart, respectively. Lane M represents the protein marker. Western blots (C–J) were scanned into gray scale and the optical density was quantified by Scion Image. Blots were probed with <t>anti-V-PPase</t> (C–F) and <t>anti-V-ATPase</t> (A-subunit) (G–J) of mung bean. Panels (C,G,D,H) show the effect of salt treatment on the amount of V-H + -ATPase and the V-H + -PPase in both cultivars. Panels (E,I,F,J) show the effect of the cultivar on the amount of V-H + -ATPase and the V-H + -PPase in both salt treatments. The molecular masses of the immunostained polypeptides are indicated (arrows). Values represent mean ± SEM of four independent replicates. Asterisks indicate a statistically significant treatment effect (Student’s t -test; P
    Control Atpase, supplied by Millipore, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/control atpase/product/Millipore
    Average 77 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    control atpase - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    88
    Thermo Fisher k atpase
    STED microscopy dissecting the localization of Na + ,K + <t>-ATPase</t> in cultured striatal neurons . The 5 × 3 mosaic shows a comparision of the confocal (left) and STED images (middle) of the Alexa-594 immunolabelled <t>α3</t> NKA in dendritic spines. Postprocessing of the raw STED data by a Richardson-Lucy deconvolution further enhances the details as shown in the right row of images. Scale bar: 500 nm
    K Atpase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k atpase/product/Thermo Fisher
    Average 88 stars, based on 38 article reviews
    Price from $9.99 to $1999.99
    k atpase - by Bioz Stars, 2020-02
    88/100 stars
      Buy from Supplier

    83
    Santa Cruz Biotechnology atpase alpha3
    STED microscopy dissecting the localization of Na + ,K + <t>-ATPase</t> in cultured striatal neurons . The 5 × 3 mosaic shows a comparision of the confocal (left) and STED images (middle) of the Alexa-594 immunolabelled <t>α3</t> NKA in dendritic spines. Postprocessing of the raw STED data by a Richardson-Lucy deconvolution further enhances the details as shown in the right row of images. Scale bar: 500 nm
    Atpase Alpha3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 83/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atpase alpha3/product/Santa Cruz Biotechnology
    Average 83 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    atpase alpha3 - by Bioz Stars, 2020-02
    83/100 stars
      Buy from Supplier

    79
    MitoSciences mitochondrial atpase
    STED microscopy dissecting the localization of Na + ,K + <t>-ATPase</t> in cultured striatal neurons . The 5 × 3 mosaic shows a comparision of the confocal (left) and STED images (middle) of the Alexa-594 immunolabelled <t>α3</t> NKA in dendritic spines. Postprocessing of the raw STED data by a Richardson-Lucy deconvolution further enhances the details as shown in the right row of images. Scale bar: 500 nm
    Mitochondrial Atpase, supplied by MitoSciences, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mitochondrial atpase/product/MitoSciences
    Average 79 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    mitochondrial atpase - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    96
    Santa Cruz Biotechnology v atpase
    ( A ) Triple immunofluorescence labeling of a kidney section from AQP2-cre + tdTomato + (PC reporter) mice showing: AQP2 (red cells, upper left);. <t>V-ATPase</t> E1 (green cells, ICs, upper right); tdTomato (blue cells, lower left) staining in inner medulla. On the merged image (lower right) most AQP2 cells were also positive for tdTomato (purple cells, white arrows) while some were negative for tdTomato (red cells, white arrowheads). The dashed box contains a tubule imaged from another kidney section that contained an isolated cell that was positive for both AQP2 and V-ATPAse E1 (Yellow cell, asterisk). Magnification 40X, confocal microscopy. ( B ) Triple immunofluorescence labeling of a kidney section from V-ATPase <t>B1-cre</t> + tdTomato + (IC reporter) mice showing: V-ATPase B1 (red cells, upper left). tdTomato (green cells, upper right) and nuclei (blue, lower left) staining in inner medulla. On the merged image (lower right) most V-ATPase B1 positive cells were also tdTomato positive (yellow cells, white arrows) while a few cells were tdTomato positive but were V-ATPase B1 negative (green cells, white arrowheads). Magnification 20X, immunofluorescent microscopy.
    V Atpase, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/v atpase/product/Santa Cruz Biotechnology
    Average 96 stars, based on 95 article reviews
    Price from $9.99 to $1999.99
    v atpase - by Bioz Stars, 2020-02
    96/100 stars
      Buy from Supplier

    79
    Trinity Biotech p97 atpase
    ( A ) Triple immunofluorescence labeling of a kidney section from AQP2-cre + tdTomato + (PC reporter) mice showing: AQP2 (red cells, upper left);. <t>V-ATPase</t> E1 (green cells, ICs, upper right); tdTomato (blue cells, lower left) staining in inner medulla. On the merged image (lower right) most AQP2 cells were also positive for tdTomato (purple cells, white arrows) while some were negative for tdTomato (red cells, white arrowheads). The dashed box contains a tubule imaged from another kidney section that contained an isolated cell that was positive for both AQP2 and V-ATPAse E1 (Yellow cell, asterisk). Magnification 40X, confocal microscopy. ( B ) Triple immunofluorescence labeling of a kidney section from V-ATPase <t>B1-cre</t> + tdTomato + (IC reporter) mice showing: V-ATPase B1 (red cells, upper left). tdTomato (green cells, upper right) and nuclei (blue, lower left) staining in inner medulla. On the merged image (lower right) most V-ATPase B1 positive cells were also tdTomato positive (yellow cells, white arrows) while a few cells were tdTomato positive but were V-ATPase B1 negative (green cells, white arrowheads). Magnification 20X, immunofluorescent microscopy.
    P97 Atpase, supplied by Trinity Biotech, used in various techniques. Bioz Stars score: 79/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p97 atpase/product/Trinity Biotech
    Average 79 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    p97 atpase - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

    77
    4Gene non atpase
    ( A ) Triple immunofluorescence labeling of a kidney section from AQP2-cre + tdTomato + (PC reporter) mice showing: AQP2 (red cells, upper left);. <t>V-ATPase</t> E1 (green cells, ICs, upper right); tdTomato (blue cells, lower left) staining in inner medulla. On the merged image (lower right) most AQP2 cells were also positive for tdTomato (purple cells, white arrows) while some were negative for tdTomato (red cells, white arrowheads). The dashed box contains a tubule imaged from another kidney section that contained an isolated cell that was positive for both AQP2 and V-ATPAse E1 (Yellow cell, asterisk). Magnification 40X, confocal microscopy. ( B ) Triple immunofluorescence labeling of a kidney section from V-ATPase <t>B1-cre</t> + tdTomato + (IC reporter) mice showing: V-ATPase B1 (red cells, upper left). tdTomato (green cells, upper right) and nuclei (blue, lower left) staining in inner medulla. On the merged image (lower right) most V-ATPase B1 positive cells were also tdTomato positive (yellow cells, white arrows) while a few cells were tdTomato positive but were V-ATPase B1 negative (green cells, white arrowheads). Magnification 20X, immunofluorescent microscopy.
    Non Atpase, supplied by 4Gene, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/non atpase/product/4Gene
    Average 77 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    non atpase - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    91
    Abcam k atpase
    ( A ) Triple immunofluorescence labeling of a kidney section from AQP2-cre + tdTomato + (PC reporter) mice showing: AQP2 (red cells, upper left);. <t>V-ATPase</t> E1 (green cells, ICs, upper right); tdTomato (blue cells, lower left) staining in inner medulla. On the merged image (lower right) most AQP2 cells were also positive for tdTomato (purple cells, white arrows) while some were negative for tdTomato (red cells, white arrowheads). The dashed box contains a tubule imaged from another kidney section that contained an isolated cell that was positive for both AQP2 and V-ATPAse E1 (Yellow cell, asterisk). Magnification 40X, confocal microscopy. ( B ) Triple immunofluorescence labeling of a kidney section from V-ATPase <t>B1-cre</t> + tdTomato + (IC reporter) mice showing: V-ATPase B1 (red cells, upper left). tdTomato (green cells, upper right) and nuclei (blue, lower left) staining in inner medulla. On the merged image (lower right) most V-ATPase B1 positive cells were also tdTomato positive (yellow cells, white arrows) while a few cells were tdTomato positive but were V-ATPase B1 negative (green cells, white arrowheads). Magnification 20X, immunofluorescent microscopy.
    K Atpase, supplied by Abcam, used in various techniques. Bioz Stars score: 91/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k atpase/product/Abcam
    Average 91 stars, based on 54 article reviews
    Price from $9.99 to $1999.99
    k atpase - by Bioz Stars, 2020-02
    91/100 stars
      Buy from Supplier

    87
    Accurate Chemical & Scientific Corporation k atpase
    ( A ) Triple immunofluorescence labeling of a kidney section from AQP2-cre + tdTomato + (PC reporter) mice showing: AQP2 (red cells, upper left);. <t>V-ATPase</t> E1 (green cells, ICs, upper right); tdTomato (blue cells, lower left) staining in inner medulla. On the merged image (lower right) most AQP2 cells were also positive for tdTomato (purple cells, white arrows) while some were negative for tdTomato (red cells, white arrowheads). The dashed box contains a tubule imaged from another kidney section that contained an isolated cell that was positive for both AQP2 and V-ATPAse E1 (Yellow cell, asterisk). Magnification 40X, confocal microscopy. ( B ) Triple immunofluorescence labeling of a kidney section from V-ATPase <t>B1-cre</t> + tdTomato + (IC reporter) mice showing: V-ATPase B1 (red cells, upper left). tdTomato (green cells, upper right) and nuclei (blue, lower left) staining in inner medulla. On the merged image (lower right) most V-ATPase B1 positive cells were also tdTomato positive (yellow cells, white arrows) while a few cells were tdTomato positive but were V-ATPase B1 negative (green cells, white arrowheads). Magnification 20X, immunofluorescent microscopy.
    K Atpase, supplied by Accurate Chemical & Scientific Corporation, used in various techniques. Bioz Stars score: 87/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k atpase/product/Accurate Chemical & Scientific Corporation
    Average 87 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    k atpase - by Bioz Stars, 2020-02
    87/100 stars
      Buy from Supplier

    92
    Cell Signaling Technology Inc k atpase
    Expression of hNGAL-R in Caco-2 BBE cells. RT-PCR for hNGAL-R and GAPDH in colon-like Caco-2 BBE cells ( A ). A PCR product of 296 bp is amplified from colon-like Caco-2 BBE cell cDNA using specific primers for human NGAL-R and reverse transcriptase (+RT), but not in the control reaction without reverse transcriptase (-RT). The housekeeping gene human GAPDH is used as a control. A 326 bp PCR product is only amplified in the presence of reverse transcriptase (+RT). Immunoblotting of colon-like Caco-2 BBE cell homogenate (Ho) and plasma membranes (PM) ( B ). Specific signals are detected in PM of colon-like Caco-2 BBE cells with antibodies against hNGAL-R (α-CT-24p3-R; 1:500) and the <t>α1-subunit</t> of Na + ,K + <t>-ATPase</t> (1:500). Live immunofluorescence staining of non-permeabilized colon- and duodenum-like Caco-2 BBE cells ( C and D ). Immunofluorescence staining with α-NT-24p3-R (1:100) reveals hNGAL-R expression (red fluorescence) at apical (asterisks) and lateral plasma membranes (arrows) of colon-like Caco-2 BBE cells ( C ). No staining for hNGAL-R is detected in duodenum-like Caco-2 BBE cells ( D ).
    K Atpase, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 92/100, based on 112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k atpase/product/Cell Signaling Technology Inc
    Average 92 stars, based on 112 article reviews
    Price from $9.99 to $1999.99
    k atpase - by Bioz Stars, 2020-02
    92/100 stars
      Buy from Supplier

    99
    Epitomics k atpase
    Expression of hNGAL-R in Caco-2 BBE cells. RT-PCR for hNGAL-R and GAPDH in colon-like Caco-2 BBE cells ( A ). A PCR product of 296 bp is amplified from colon-like Caco-2 BBE cell cDNA using specific primers for human NGAL-R and reverse transcriptase (+RT), but not in the control reaction without reverse transcriptase (-RT). The housekeeping gene human GAPDH is used as a control. A 326 bp PCR product is only amplified in the presence of reverse transcriptase (+RT). Immunoblotting of colon-like Caco-2 BBE cell homogenate (Ho) and plasma membranes (PM) ( B ). Specific signals are detected in PM of colon-like Caco-2 BBE cells with antibodies against hNGAL-R (α-CT-24p3-R; 1:500) and the <t>α1-subunit</t> of Na + ,K + <t>-ATPase</t> (1:500). Live immunofluorescence staining of non-permeabilized colon- and duodenum-like Caco-2 BBE cells ( C and D ). Immunofluorescence staining with α-NT-24p3-R (1:100) reveals hNGAL-R expression (red fluorescence) at apical (asterisks) and lateral plasma membranes (arrows) of colon-like Caco-2 BBE cells ( C ). No staining for hNGAL-R is detected in duodenum-like Caco-2 BBE cells ( D ).
    K Atpase, supplied by Epitomics, used in various techniques. Bioz Stars score: 99/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k atpase/product/Epitomics
    Average 99 stars, based on 54 article reviews
    Price from $9.99 to $1999.99
    k atpase - by Bioz Stars, 2020-02
    99/100 stars
      Buy from Supplier

    85
    Millipore atpase assays
    Immunostaining of MCF10a cells overexpressing <t>subunit</t> a isoforms using an antibody against <t>V-ATPase.</t> MCF10a cells were grown as a monolayer on coverslips in 6-well plates. Cells were immunostained using an antibody against subunit A of V-ATPase. Images
    Atpase Assays, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atpase assays/product/Millipore
    Average 85 stars, based on 35 article reviews
    Price from $9.99 to $1999.99
    atpase assays - by Bioz Stars, 2020-02
    85/100 stars
      Buy from Supplier

    86
    Synaptic Systems v atpase
    Immunostaining of MCF10a cells overexpressing <t>subunit</t> a isoforms using an antibody against <t>V-ATPase.</t> MCF10a cells were grown as a monolayer on coverslips in 6-well plates. Cells were immunostained using an antibody against subunit A of V-ATPase. Images
    V Atpase, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 86/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/v atpase/product/Synaptic Systems
    Average 86 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    v atpase - by Bioz Stars, 2020-02
    86/100 stars
      Buy from Supplier

    85
    Horizon Discovery v atpase
    Intracellular localization of subunit <t>a3</t> and markers for the Golgi apparatus and lysosomes in breast cancer cells MCF10a, MCF10CA1a, MB231, and SUM149 cells were grown on poly-d-lysine coated coverslips. To assess whether a3 localizes to the Golgi and lysosomes, cells were fixed, permeabilized, and immunostained using antibodies against subunit a3 of the <t>V-ATPase</t> and Giantin or LAMP2 as markers for the Golgi and lysosomes, respectively. Cells were then incubated with secondary antibodies as described under Materials and Methods. Images were taken with identical exposure times and antibody concentrations. A. Representative images of each of the cell lines stained for the Golgi apparatus marker Giantin ( left ), subunit a3 ( second from left ), the merged images ( second from right ), and the merged image magnified 3.5X ( labeled ‘Zoom’, far right ). B. Representative images of each of the cell lines stained for the lysosomal marker LAMP2 ( left ), subunit a3 ( second from left ), the merged images ( second from right ), and the inset of the merged image magnified 3.5X ( labeled ‘Zoom’, far right ). Colocalization is indicated by the presence of yellow pixels. The images in A and B depict representative staining from a minimum of two separate experiments, with an average of 50 cells imaged per experiment for each condition tested.
    V Atpase, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/v atpase/product/Horizon Discovery
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    v atpase - by Bioz Stars, 2020-02
    85/100 stars
      Buy from Supplier

    77
    Seca Inc cytoplasmic atpase
    Intracellular localization of subunit <t>a3</t> and markers for the Golgi apparatus and lysosomes in breast cancer cells MCF10a, MCF10CA1a, MB231, and SUM149 cells were grown on poly-d-lysine coated coverslips. To assess whether a3 localizes to the Golgi and lysosomes, cells were fixed, permeabilized, and immunostained using antibodies against subunit a3 of the <t>V-ATPase</t> and Giantin or LAMP2 as markers for the Golgi and lysosomes, respectively. Cells were then incubated with secondary antibodies as described under Materials and Methods. Images were taken with identical exposure times and antibody concentrations. A. Representative images of each of the cell lines stained for the Golgi apparatus marker Giantin ( left ), subunit a3 ( second from left ), the merged images ( second from right ), and the merged image magnified 3.5X ( labeled ‘Zoom’, far right ). B. Representative images of each of the cell lines stained for the lysosomal marker LAMP2 ( left ), subunit a3 ( second from left ), the merged images ( second from right ), and the inset of the merged image magnified 3.5X ( labeled ‘Zoom’, far right ). Colocalization is indicated by the presence of yellow pixels. The images in A and B depict representative staining from a minimum of two separate experiments, with an average of 50 cells imaged per experiment for each condition tested.
    Cytoplasmic Atpase, supplied by Seca Inc, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cytoplasmic atpase/product/Seca Inc
    Average 77 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    cytoplasmic atpase - by Bioz Stars, 2020-02
    77/100 stars
      Buy from Supplier

    88
    Upstate Biotechnology Inc k atpase
    Colocalization of subunits of ENaC and Na + -, K + <t>-ATPase</t> with markers specific for TI and TII cells. The preparations of isolated cells used in these experiments contain not only TI cells and TII cells, but various other lung cells liberated by enzymatic digestion, including airway cells, macrophages, and cells from the vascular and interstitial compartments. Immunocytochemistry was performed as described in Methods . The same field of cells is shown in each row, stained with three antibodies and three different fluorophores specific for each antibody. The colors shown in each column are the result of scanning with the appropriate laser for each fluorophore. In the various columns, one can appreciate staining for ( a ) antibodies against various channel or pump proteins (blue color); ( b ) RTI40, a marker for TI cells (red color); and ( c ) RTII70, a marker for TII cells (green color). Column d shows a composite image, created by superimposition of the three separate images shown in columns a , b , and c . Arrows indicate one representative TI cell, one TII cell, and a cell that is neither a TI nor a TII cell. Every TI and TII cell expressed all of the pump/channel proteins. This fact can be appreciated most easily when one looks in column d at the spectral shifts caused by overlapping red (TI) or green (TII) markers with the blue (pump/channel) protein. In this composite image, colocalization of a transport protein subunit (ENaC or Na + -, K + -ATPase) with either TI or TII cell plasma membrane causes a color shift, indicating colocalization of the protein subunit with TI or TII plasma membranes. For example, combination of blue (subunit) with red (TI) is pinkish purple, whereas a combination of blue (subunit) with green (TII) is bluish green. Specific antibodies are labeled in rows 1–5: row 1, αENaC; row 2, βENaC; row 3, γENaC; row 4, <t>α</t> 1 -Na + -, K + -ATPase; row 5, β 1 -Na + -, K + -ATPase; row 6, Cos7 cells stained for αENaC; and row 7, no primary antibody control panel with mixed lung cells.
    K Atpase, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 88/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k atpase/product/Upstate Biotechnology Inc
    Average 88 stars, based on 37 article reviews
    Price from $9.99 to $1999.99
    k atpase - by Bioz Stars, 2020-02
    88/100 stars
      Buy from Supplier

    78
    Cytoskeleton Inc atpase assay kinesin atpase assay kit
    Colocalization of subunits of ENaC and Na + -, K + <t>-ATPase</t> with markers specific for TI and TII cells. The preparations of isolated cells used in these experiments contain not only TI cells and TII cells, but various other lung cells liberated by enzymatic digestion, including airway cells, macrophages, and cells from the vascular and interstitial compartments. Immunocytochemistry was performed as described in Methods . The same field of cells is shown in each row, stained with three antibodies and three different fluorophores specific for each antibody. The colors shown in each column are the result of scanning with the appropriate laser for each fluorophore. In the various columns, one can appreciate staining for ( a ) antibodies against various channel or pump proteins (blue color); ( b ) RTI40, a marker for TI cells (red color); and ( c ) RTII70, a marker for TII cells (green color). Column d shows a composite image, created by superimposition of the three separate images shown in columns a , b , and c . Arrows indicate one representative TI cell, one TII cell, and a cell that is neither a TI nor a TII cell. Every TI and TII cell expressed all of the pump/channel proteins. This fact can be appreciated most easily when one looks in column d at the spectral shifts caused by overlapping red (TI) or green (TII) markers with the blue (pump/channel) protein. In this composite image, colocalization of a transport protein subunit (ENaC or Na + -, K + -ATPase) with either TI or TII cell plasma membrane causes a color shift, indicating colocalization of the protein subunit with TI or TII plasma membranes. For example, combination of blue (subunit) with red (TI) is pinkish purple, whereas a combination of blue (subunit) with green (TII) is bluish green. Specific antibodies are labeled in rows 1–5: row 1, αENaC; row 2, βENaC; row 3, γENaC; row 4, <t>α</t> 1 -Na + -, K + -ATPase; row 5, β 1 -Na + -, K + -ATPase; row 6, Cos7 cells stained for αENaC; and row 7, no primary antibody control panel with mixed lung cells.
    Atpase Assay Kinesin Atpase Assay Kit, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atpase assay kinesin atpase assay kit/product/Cytoskeleton Inc
    Average 78 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    atpase assay kinesin atpase assay kit - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    94
    Abcam atpase
    Expression of candidate cysteine transporters in rat heart. a ASCT1. The top panel shows the results of a representative RT-PCR. Lane 1 contains a ladder; lane 2 contains cDNA isolated from cardiomyocytes; lane 3 contains RNA isolated from cardiomyocytes; lane 4 contains cDNA isolated from brain; and lane 5 contains water. The middle panel shows Western blotting results. Lanes 1 and 2 contain brain synaptosomal membrane vesicles (BSMV); lanes 3 and 4 contain cardiac sarcolemmal vesicles (CSV). The bottom panel shows the same membrane following stripping and re-probing for the <t>α</t> 1 subunit of Na + , K + , <t>ATPase.</t> b ASCT2. The top panel shows the results of a representative RT-PCR. Lane 1 contains a ladder; lane 2 contains cDNA isolated from brain; lane 3 contains RNA isolated from cardiomyocytes; lane 4 contains water; lanes 5 and 6 contain cDNA isolated from cardiomyocytes. The middle panel shows Western blot results. Lane 1 contains a ladder; lane 2 contains BSMV; lane 3 contains CSV. The bottom panel shows the same membrane following stripping and re-probing for the α 1 subunit of Na + , K + , ATPase. Lanes 1 and 2 BSMV; lanes 3 and 4 CSV. c SNAT1. The top panel shows RT-PCR results. Lane 1 contains a ladder; lane 2 contains cDNA isolated from cardiomyocytes; lane 3 contains cDNA isolated from the brain. The middle panel shows Western blot results. Lane 1 contains a ladder; lane 2 contains CSV; lane 3 contains cardiomyocytes; lane 4 contains BSMV. The bottom panel shows the same membrane following stripping and re-probing for the α 1 subunit of Na + , K + , ATPase. Lane 1 contains CSV; lane 2 contains cardiomyocytes; lane 3 contains BSMV. d SNAT2. The top panel shows RT-PCR results. Lane 1 contains a ladder; lane 2 contains cDNA isolated from cardiomyocytes; lane 3 contains water; lane 4 contains cDNA isolated from the brain; lane 5 contains RNA isolated from cardiomyocytes. The middle panel shows Western blot results. Lane 1 contains a ladder; lane 2 contains L6 myotube cells; lane 3 contains BSMV; lane 4 contains cardiomyocytes; lane 5 contains CSV. The bottom panel shows the same membrane following stripping and re-probing for the α 1 subunit of Na + , K + , ATPase. All of the experiments were repeated three to five times with similar results
    Atpase, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atpase/product/Abcam
    Average 94 stars, based on 41 article reviews
    Price from $9.99 to $1999.99
    atpase - by Bioz Stars, 2020-02
    94/100 stars
      Buy from Supplier

    Image Search Results


    Decreased K ATP channel subunit expression in ankyrin-B +/− hearts. Detergent-soluble ankyrin-B +/− heart lysates expressed significantly less ankyrin-B ( A ), Kir6.2 ( B ), SUR1 ( C ), SUR2A ( D ), Na/K-ATPase ( H ), and NCX1 ( I ). There were no significant differences in the expression of ankyrin-G ( E ), Kir6.1 ( F ), or NHERF1 (loading control; G ). J , quantitative data demonstrating a significant decrease in the expression of ankyrin-B, NCX1, Na/K-ATPase, and K ATP channel proteins in ankyrin-B +/− compared with wild-type detergent-soluble mouse heart lysates. Expression of proteins was normalized to wild-type expression. IB , immunoblot. *, p

    Journal: The Journal of Biological Chemistry

    Article Title: Ankyrin-B Regulates Kir6.2 Membrane Expression and Function in Heart *

    doi: 10.1074/jbc.M110.147868

    Figure Lengend Snippet: Decreased K ATP channel subunit expression in ankyrin-B +/− hearts. Detergent-soluble ankyrin-B +/− heart lysates expressed significantly less ankyrin-B ( A ), Kir6.2 ( B ), SUR1 ( C ), SUR2A ( D ), Na/K-ATPase ( H ), and NCX1 ( I ). There were no significant differences in the expression of ankyrin-G ( E ), Kir6.1 ( F ), or NHERF1 (loading control; G ). J , quantitative data demonstrating a significant decrease in the expression of ankyrin-B, NCX1, Na/K-ATPase, and K ATP channel proteins in ankyrin-B +/− compared with wild-type detergent-soluble mouse heart lysates. Expression of proteins was normalized to wild-type expression. IB , immunoblot. *, p

    Article Snippet: Protein A-conjugated agarose beads (Rockland Immunochemicals, Inc.) were incubated with either control IgG or affinity-purified anti-ankyrin-B, anti-Kir6.2, anti-NCX1, or anti-Na/K-ATPase antibody in co-immunoprecipitation binding buffer (PBS with 0.1% Triton X-100 and protease inhibitor mixture (Sigma)) for 12 h at 4 °C.

    Techniques: Expressing

    Ankyrin-B forms a macromolecular complex with K ATP , Na/K-ATPase, and NCX that is reduced in ankyrin-B +/− heart. A–D , detergent-soluble lysates from wild-type and ankyrin-B +/− mouse hearts were used for co-immunoprecipitations with the indicated antibodies. IB , immunoblot; IP , immunoprecipitation. Co-immunoprecipitations of ankyrin-B +/− lysates used doubled amounts of input lysate to compensate for the reduction of ankyrin-B. E and F , detergent-soluble lysates from wild-type and ankyrin-B +/− mouse hearts were used for control co-immunoprecipitations, demonstrating no interaction of Na/K-ATPase ( NKA ) and NCX with SERCA2, Ca v 1.2, and Na v 1.5.

    Journal: The Journal of Biological Chemistry

    Article Title: Ankyrin-B Regulates Kir6.2 Membrane Expression and Function in Heart *

    doi: 10.1074/jbc.M110.147868

    Figure Lengend Snippet: Ankyrin-B forms a macromolecular complex with K ATP , Na/K-ATPase, and NCX that is reduced in ankyrin-B +/− heart. A–D , detergent-soluble lysates from wild-type and ankyrin-B +/− mouse hearts were used for co-immunoprecipitations with the indicated antibodies. IB , immunoblot; IP , immunoprecipitation. Co-immunoprecipitations of ankyrin-B +/− lysates used doubled amounts of input lysate to compensate for the reduction of ankyrin-B. E and F , detergent-soluble lysates from wild-type and ankyrin-B +/− mouse hearts were used for control co-immunoprecipitations, demonstrating no interaction of Na/K-ATPase ( NKA ) and NCX with SERCA2, Ca v 1.2, and Na v 1.5.

    Article Snippet: Protein A-conjugated agarose beads (Rockland Immunochemicals, Inc.) were incubated with either control IgG or affinity-purified anti-ankyrin-B, anti-Kir6.2, anti-NCX1, or anti-Na/K-ATPase antibody in co-immunoprecipitation binding buffer (PBS with 0.1% Triton X-100 and protease inhibitor mixture (Sigma)) for 12 h at 4 °C.

    Techniques: Immunoprecipitation

    Muscles of LAMA2 MD patients and dy W /dy W mice contain high amounts of laminin-α4 and show deficits in BM ( A ) Representative immunofluorescence images (magnified images in insets) of human muscle biopsy cross sections stained for laminin-α4 and laminin-β1γ1. n = 3 LAMA2 MD patients or healthy controls. ( B ) Western blot analysis and quantification of laminin chains in human muscle biopsies. α-Actinin was used as loading control. n = 4 controls, n = 3 LAMA2 MD patients. ( C ) Representative immunofluorescence images (magnified images in insets) of triceps muscle from 8-week-old mice stained for laminin-α4 and laminin-β1γ1. n = 4 mice per genotype. ( D ) Western blot analysis and quantification of laminin-α4 (left graph), laminin-β1γ1 (middle graph), and Lama4 transcripts (right graph) from triceps muscle of 8-week-old mice. n = 3 mice per genotype (Western blot) and n = 4 mice per genotype (quantitative reverse transcription polymerase chain reaction). ( E ) Schematic of subcellular fractionation. ( F ) Western blot analysis of S1 to S4 of a muscle biopsy from a control patient. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and Na + /K + -ATPase (sodium-potassium adenosine triphosphatase) were used as markers for cytosolic and membrane proteins, respectively. ( G ) Western blot analysis of S3 and S4 fractions from control and LAMA2 MD patient muscle biopsies probed for the indicated proteins. Right: Ratio of the laminin-β1γ1 intensity in S3 and S4 fraction. n = 4 healthy controls, n = 3 LAMA2 MD patients. Data are means ± SEM. * P

    Journal: Science translational medicine

    Article Title: Linker proteins restore basement membrane and correct LAMA2-related muscular dystrophy in mice

    doi: 10.1126/scitranslmed.aal4649

    Figure Lengend Snippet: Muscles of LAMA2 MD patients and dy W /dy W mice contain high amounts of laminin-α4 and show deficits in BM ( A ) Representative immunofluorescence images (magnified images in insets) of human muscle biopsy cross sections stained for laminin-α4 and laminin-β1γ1. n = 3 LAMA2 MD patients or healthy controls. ( B ) Western blot analysis and quantification of laminin chains in human muscle biopsies. α-Actinin was used as loading control. n = 4 controls, n = 3 LAMA2 MD patients. ( C ) Representative immunofluorescence images (magnified images in insets) of triceps muscle from 8-week-old mice stained for laminin-α4 and laminin-β1γ1. n = 4 mice per genotype. ( D ) Western blot analysis and quantification of laminin-α4 (left graph), laminin-β1γ1 (middle graph), and Lama4 transcripts (right graph) from triceps muscle of 8-week-old mice. n = 3 mice per genotype (Western blot) and n = 4 mice per genotype (quantitative reverse transcription polymerase chain reaction). ( E ) Schematic of subcellular fractionation. ( F ) Western blot analysis of S1 to S4 of a muscle biopsy from a control patient. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and Na + /K + -ATPase (sodium-potassium adenosine triphosphatase) were used as markers for cytosolic and membrane proteins, respectively. ( G ) Western blot analysis of S3 and S4 fractions from control and LAMA2 MD patient muscle biopsies probed for the indicated proteins. Right: Ratio of the laminin-β1γ1 intensity in S3 and S4 fraction. n = 4 healthy controls, n = 3 LAMA2 MD patients. Data are means ± SEM. * P

    Article Snippet: For immunostaining and Western blot analysis, the following antibodies were used: α-actinin (1:5000; Sigma, catalog no. A7732), agrin [chicken, for detection of mag, produced in-house , 1:1000 for Western blots, 1:200 for immunostainings], eMHC (1:100 for Western blot, 1:1200 for immunostainings; developed by H. Blau, obtained from Developmental Studies Hybridoma Bank, catalog no. F1.652), F4/80 (1:100; Abcam, catalog no. ab6640), laminin-α1 (for detection of αLNNd by Western blot, 1:2000; R & D Systems, catalog no. AF4187), αLNNd [for detection of αLNNd by immunostainings, 1:100 ( )], laminin-α2 [for Western blot analysis , 1:500], laminin-α2 (N-terminal, for immunostainings of human samples, clone 4H8-2, 1:400; Sigma, catalog no. L0663), laminin-α2 (C-terminal, for immunostainings of human samples, clone 5H2, 1:5000; Merck Millipore, catalog no. MAB1922), laminin-α4 ( ) (1:1000 for Western blots, 1:200 for immunostainings), laminin-α5 [clone 504, gift from L. Sorokin , 1:1000], laminin-β1γ1 (1:1000 for Western blots, 1:100 for immunostainings; Sigma, catalog no. L9393), laminin-γ1 (1:200; Millipore, catalog no. MAB1914), Na+ /K+ -ATPase (1:1000; Cell Signaling, catalog no. 3010), GAPDH (1:1000; Cell Signaling, catalog no. 2118), and CD31 (1:100; Abcam, catalog no. ab9498).

    Techniques: Mouse Assay, Immunofluorescence, Staining, Western Blot, Reverse Transcription Polymerase Chain Reaction, Fractionation

    A–E : phosphorylation of Akt and protein levels of total and plasma membrane (PM) glucose transporters (GLUT4 and GLUT3) in the ventromedial hypothalamus (VMH) of nondiabetic control (CON) and streptozotocin-diabetic (DIAB) rats in the basal state (i.e., not subjected to hyperinsulinemic clamp) and during acute intravenous infusion of insulin during hyperinsulinemic clamp. Nondiabetic control rats had been infused chronically (i.e., for 2 wk) with artificial cerebrospinal fluid (aCSF) into the VMH (CON/VMHaCSF); diabetic rats had been infused chronically with aCSF (DIAB/VMHaCSF) or regular insulin (DIAB/VMH-Ins) into the VMH. Actin and Na-K-ATPase proteins were used as internal control to normalize data for total and PM GLUT levels, respectively. F : correlation analysis of total GLUT4 with peak epinephrine levels in nondiabetic controls (CON/VMHaCSF) and diabetic rats (DIAB/VMHaCSF and DIAB/VMH-Ins). GLUT4 content is expressed relative to control rat GLUT4 content during the basal state (CON/VMHaCSF). Values are means ± SE ( n = 8/group). * P

    Journal: American Journal of Physiology - Endocrinology and Metabolism

    Article Title: Insulin regulates GLUT4 in the ventromedial hypothalamus to restore the sympathoadrenal response to hypoglycemia in diabetic rats

    doi: 10.1152/ajpendo.00324.2018

    Figure Lengend Snippet: A–E : phosphorylation of Akt and protein levels of total and plasma membrane (PM) glucose transporters (GLUT4 and GLUT3) in the ventromedial hypothalamus (VMH) of nondiabetic control (CON) and streptozotocin-diabetic (DIAB) rats in the basal state (i.e., not subjected to hyperinsulinemic clamp) and during acute intravenous infusion of insulin during hyperinsulinemic clamp. Nondiabetic control rats had been infused chronically (i.e., for 2 wk) with artificial cerebrospinal fluid (aCSF) into the VMH (CON/VMHaCSF); diabetic rats had been infused chronically with aCSF (DIAB/VMHaCSF) or regular insulin (DIAB/VMH-Ins) into the VMH. Actin and Na-K-ATPase proteins were used as internal control to normalize data for total and PM GLUT levels, respectively. F : correlation analysis of total GLUT4 with peak epinephrine levels in nondiabetic controls (CON/VMHaCSF) and diabetic rats (DIAB/VMHaCSF and DIAB/VMH-Ins). GLUT4 content is expressed relative to control rat GLUT4 content during the basal state (CON/VMHaCSF). Values are means ± SE ( n = 8/group). * P

    Article Snippet: Polyvinylidene difluoride (PVDF) membranes (Millipore) were incubated with the following primary antibodies: anti-phosphorylated Akt (1:1,000 dilution; Cell Signaling Technology, Danvers, MA), anti-Akt (1:1,000 dilution; Cell Signaling Technology), anti-GLUT3 (1:1,000; Santa Cruz Biotechnology, Santa Cruz, CA), anti-GLUT4 (1:1,000 dilution; Millipore), β-actin (1:1,000, Sigma-Aldrich, St. Louis, MO), and Na-K-ATPase (1:1,000 dilution; Cell Signaling Technology).

    Techniques:

    The immunoblots and relative intensities of Prdx6 in membrane-fraction proteins of livers of the fresh water (FW) and seawater (SW) milkfish acclimated to 28°C (white bar) and 18°C (stripe bar) for 1 week, respectively . In the representative immunoblot, single immunoreactive bands at 100 and 25 kDa represent Na + , K + -ATPase (NKA), and Prdx6, respectively. The NKA was used as the loading control of the membrane fraction. Different letters (a and b) indicate significant differences between the 28° and 18°C group, and (x and y) indicate significant differences between the FW and SW group. Values are means ± SEM, n = 6. The Student's t -test pairwise comparison was used following two-way ANOVA, P

    Journal: Frontiers in Physiology

    Article Title: The Antioxidant Peroxiredoxin 6 (Prdx6) Exhibits Different Profiles in the Livers of Seawater- and Fresh Water-Acclimated Milkfish, Chanos chanos, upon Hypothermal Challenge

    doi: 10.3389/fphys.2016.00580

    Figure Lengend Snippet: The immunoblots and relative intensities of Prdx6 in membrane-fraction proteins of livers of the fresh water (FW) and seawater (SW) milkfish acclimated to 28°C (white bar) and 18°C (stripe bar) for 1 week, respectively . In the representative immunoblot, single immunoreactive bands at 100 and 25 kDa represent Na + , K + -ATPase (NKA), and Prdx6, respectively. The NKA was used as the loading control of the membrane fraction. Different letters (a and b) indicate significant differences between the 28° and 18°C group, and (x and y) indicate significant differences between the FW and SW group. Values are means ± SEM, n = 6. The Student's t -test pairwise comparison was used following two-way ANOVA, P

    Article Snippet: Then, the immunoblotting was performed as described above except that Na+ , K+ -ATPase (NKA; α5, Developmental Studies Hybridoma Bank, Iowa City, IA, USA) was used as the loading control.

    Techniques: Western Blot

    Electrophoretic polypeptide pattern and Western blot analysis of tonoplast proteins from the potato cultivars Desiree and Mozart. Tonoplast vesicles were isolated from leaves of plants grown in the absence and presence of NaCl as indicated in the figure by 0 and 60 mM, respectively. (A,B) Coomassie blue stained gel of tonoplast proteins from Desiree and Mozart, respectively. Lane M represents the protein marker. Western blots (C–J) were scanned into gray scale and the optical density was quantified by Scion Image. Blots were probed with anti-V-PPase (C–F) and anti-V-ATPase (A-subunit) (G–J) of mung bean. Panels (C,G,D,H) show the effect of salt treatment on the amount of V-H + -ATPase and the V-H + -PPase in both cultivars. Panels (E,I,F,J) show the effect of the cultivar on the amount of V-H + -ATPase and the V-H + -PPase in both salt treatments. The molecular masses of the immunostained polypeptides are indicated (arrows). Values represent mean ± SEM of four independent replicates. Asterisks indicate a statistically significant treatment effect (Student’s t -test; P

    Journal: Frontiers in Plant Science

    Article Title: Salinity Tolerance of Two Potato Cultivars (Solanum tuberosum) Correlates With Differences in Vacuolar Transport Activity

    doi: 10.3389/fpls.2018.00737

    Figure Lengend Snippet: Electrophoretic polypeptide pattern and Western blot analysis of tonoplast proteins from the potato cultivars Desiree and Mozart. Tonoplast vesicles were isolated from leaves of plants grown in the absence and presence of NaCl as indicated in the figure by 0 and 60 mM, respectively. (A,B) Coomassie blue stained gel of tonoplast proteins from Desiree and Mozart, respectively. Lane M represents the protein marker. Western blots (C–J) were scanned into gray scale and the optical density was quantified by Scion Image. Blots were probed with anti-V-PPase (C–F) and anti-V-ATPase (A-subunit) (G–J) of mung bean. Panels (C,G,D,H) show the effect of salt treatment on the amount of V-H + -ATPase and the V-H + -PPase in both cultivars. Panels (E,I,F,J) show the effect of the cultivar on the amount of V-H + -ATPase and the V-H + -PPase in both salt treatments. The molecular masses of the immunostained polypeptides are indicated (arrows). Values represent mean ± SEM of four independent replicates. Asterisks indicate a statistically significant treatment effect (Student’s t -test; P

    Article Snippet: The primary antibodies were specific for subunit A of the V-ATPase and the V-PPase (AS09 467 and AS09-465, respectively, Agrisera, Sweden).

    Techniques: Western Blot, Isolation, Staining, Marker

    STED microscopy dissecting the localization of Na + ,K + -ATPase in cultured striatal neurons . The 5 × 3 mosaic shows a comparision of the confocal (left) and STED images (middle) of the Alexa-594 immunolabelled α3 NKA in dendritic spines. Postprocessing of the raw STED data by a Richardson-Lucy deconvolution further enhances the details as shown in the right row of images. Scale bar: 500 nm

    Journal: BMC Neuroscience

    Article Title: Spatial distribution of Na+-K+-ATPase in dendritic spines dissected by nanoscale superresolution STED microscopy

    doi: 10.1186/1471-2202-12-16

    Figure Lengend Snippet: STED microscopy dissecting the localization of Na + ,K + -ATPase in cultured striatal neurons . The 5 × 3 mosaic shows a comparision of the confocal (left) and STED images (middle) of the Alexa-594 immunolabelled α3 NKA in dendritic spines. Postprocessing of the raw STED data by a Richardson-Lucy deconvolution further enhances the details as shown in the right row of images. Scale bar: 500 nm

    Article Snippet: Antibodies In both imaging and biochemical assays the well characterized mouse monoclonal anti-α3 Na + ,K+ -ATPase (Affinity bioreagents, MA3-915) was used [ ].

    Techniques: Microscopy, Cell Culture

    ( A ) Triple immunofluorescence labeling of a kidney section from AQP2-cre + tdTomato + (PC reporter) mice showing: AQP2 (red cells, upper left);. V-ATPase E1 (green cells, ICs, upper right); tdTomato (blue cells, lower left) staining in inner medulla. On the merged image (lower right) most AQP2 cells were also positive for tdTomato (purple cells, white arrows) while some were negative for tdTomato (red cells, white arrowheads). The dashed box contains a tubule imaged from another kidney section that contained an isolated cell that was positive for both AQP2 and V-ATPAse E1 (Yellow cell, asterisk). Magnification 40X, confocal microscopy. ( B ) Triple immunofluorescence labeling of a kidney section from V-ATPase B1-cre + tdTomato + (IC reporter) mice showing: V-ATPase B1 (red cells, upper left). tdTomato (green cells, upper right) and nuclei (blue, lower left) staining in inner medulla. On the merged image (lower right) most V-ATPase B1 positive cells were also tdTomato positive (yellow cells, white arrows) while a few cells were tdTomato positive but were V-ATPase B1 negative (green cells, white arrowheads). Magnification 20X, immunofluorescent microscopy.

    Journal: Scientific Reports

    Article Title: Whole Transcriptome Analysis of Renal Intercalated Cells Predicts Lipopolysaccharide Mediated Inhibition of Retinoid X Receptor alpha Function

    doi: 10.1038/s41598-018-36921-z

    Figure Lengend Snippet: ( A ) Triple immunofluorescence labeling of a kidney section from AQP2-cre + tdTomato + (PC reporter) mice showing: AQP2 (red cells, upper left);. V-ATPase E1 (green cells, ICs, upper right); tdTomato (blue cells, lower left) staining in inner medulla. On the merged image (lower right) most AQP2 cells were also positive for tdTomato (purple cells, white arrows) while some were negative for tdTomato (red cells, white arrowheads). The dashed box contains a tubule imaged from another kidney section that contained an isolated cell that was positive for both AQP2 and V-ATPAse E1 (Yellow cell, asterisk). Magnification 40X, confocal microscopy. ( B ) Triple immunofluorescence labeling of a kidney section from V-ATPase B1-cre + tdTomato + (IC reporter) mice showing: V-ATPase B1 (red cells, upper left). tdTomato (green cells, upper right) and nuclei (blue, lower left) staining in inner medulla. On the merged image (lower right) most V-ATPase B1 positive cells were also tdTomato positive (yellow cells, white arrows) while a few cells were tdTomato positive but were V-ATPase B1 negative (green cells, white arrowheads). Magnification 20X, immunofluorescent microscopy.

    Article Snippet: V-ATPase-cre mice : In this mice, to visualize, V-ATPase, goat anti-mouse V-ATPase B1/B2 antibody (E20) (sc21209, 1:50 dilution, Santa Cruz, CA), to visualize tdTomato, anti-RFP-FITC conjugated antibody (ab34764, 1:200 dilution, abcam, MA) were incubated overnight at 4 °C.

    Techniques: Immunofluorescence, Labeling, Mouse Assay, Staining, Isolation, Confocal Microscopy, Microscopy

    Expression of hNGAL-R in Caco-2 BBE cells. RT-PCR for hNGAL-R and GAPDH in colon-like Caco-2 BBE cells ( A ). A PCR product of 296 bp is amplified from colon-like Caco-2 BBE cell cDNA using specific primers for human NGAL-R and reverse transcriptase (+RT), but not in the control reaction without reverse transcriptase (-RT). The housekeeping gene human GAPDH is used as a control. A 326 bp PCR product is only amplified in the presence of reverse transcriptase (+RT). Immunoblotting of colon-like Caco-2 BBE cell homogenate (Ho) and plasma membranes (PM) ( B ). Specific signals are detected in PM of colon-like Caco-2 BBE cells with antibodies against hNGAL-R (α-CT-24p3-R; 1:500) and the α1-subunit of Na + ,K + -ATPase (1:500). Live immunofluorescence staining of non-permeabilized colon- and duodenum-like Caco-2 BBE cells ( C and D ). Immunofluorescence staining with α-NT-24p3-R (1:100) reveals hNGAL-R expression (red fluorescence) at apical (asterisks) and lateral plasma membranes (arrows) of colon-like Caco-2 BBE cells ( C ). No staining for hNGAL-R is detected in duodenum-like Caco-2 BBE cells ( D ).

    Journal: PLoS ONE

    Article Title: Expression and Function of the Lipocalin-2 (24p3/NGAL) Receptor in Rodent and Human Intestinal Epithelia

    doi: 10.1371/journal.pone.0071586

    Figure Lengend Snippet: Expression of hNGAL-R in Caco-2 BBE cells. RT-PCR for hNGAL-R and GAPDH in colon-like Caco-2 BBE cells ( A ). A PCR product of 296 bp is amplified from colon-like Caco-2 BBE cell cDNA using specific primers for human NGAL-R and reverse transcriptase (+RT), but not in the control reaction without reverse transcriptase (-RT). The housekeeping gene human GAPDH is used as a control. A 326 bp PCR product is only amplified in the presence of reverse transcriptase (+RT). Immunoblotting of colon-like Caco-2 BBE cell homogenate (Ho) and plasma membranes (PM) ( B ). Specific signals are detected in PM of colon-like Caco-2 BBE cells with antibodies against hNGAL-R (α-CT-24p3-R; 1:500) and the α1-subunit of Na + ,K + -ATPase (1:500). Live immunofluorescence staining of non-permeabilized colon- and duodenum-like Caco-2 BBE cells ( C and D ). Immunofluorescence staining with α-NT-24p3-R (1:100) reveals hNGAL-R expression (red fluorescence) at apical (asterisks) and lateral plasma membranes (arrows) of colon-like Caco-2 BBE cells ( C ). No staining for hNGAL-R is detected in duodenum-like Caco-2 BBE cells ( D ).

    Article Snippet: A rabbit polyclonal antibody against the α1-subunit of Na+ ,K+ -ATPase (Cell Signaling Technology; 1:500), a mouse polyclonal anti β-actin antibody (1: 5,000 v/v; Sigma-Aldrich) and a rabbit polyclonal anti DMT-1 antibody (1: 500 v/v [ ]) were used for immunoblotting.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Amplification, Immunofluorescence, Staining, Fluorescence

    Immunostaining of MCF10a cells overexpressing subunit a isoforms using an antibody against V-ATPase. MCF10a cells were grown as a monolayer on coverslips in 6-well plates. Cells were immunostained using an antibody against subunit A of V-ATPase. Images

    Journal: The Journal of Biological Chemistry

    Article Title: The Function of Vacuolar ATPase (V-ATPase) a Subunit Isoforms in Invasiveness of MCF10a and MCF10CA1a Human Breast Cancer Cells *

    doi: 10.1074/jbc.M113.503771

    Figure Lengend Snippet: Immunostaining of MCF10a cells overexpressing subunit a isoforms using an antibody against V-ATPase. MCF10a cells were grown as a monolayer on coverslips in 6-well plates. Cells were immunostained using an antibody against subunit A of V-ATPase. Images

    Article Snippet: A monoclonal antibody raised against the V-ATPase subunit A (Sigma, clone 4F5) was used to determine the in situ localization of V-ATPases.

    Techniques: Immunostaining

    Immunostaining of MCF10a and MCF10CA1a cells using an antibody against V-ATPase. MCF10a and MCF10CA1a cells were grown as a monolayer on coverslips in 6-well plates. Cells were immunostained using an antibody against subunit A of V-ATPase (part of the

    Journal: The Journal of Biological Chemistry

    Article Title: The Function of Vacuolar ATPase (V-ATPase) a Subunit Isoforms in Invasiveness of MCF10a and MCF10CA1a Human Breast Cancer Cells *

    doi: 10.1074/jbc.M113.503771

    Figure Lengend Snippet: Immunostaining of MCF10a and MCF10CA1a cells using an antibody against V-ATPase. MCF10a and MCF10CA1a cells were grown as a monolayer on coverslips in 6-well plates. Cells were immunostained using an antibody against subunit A of V-ATPase (part of the

    Article Snippet: A monoclonal antibody raised against the V-ATPase subunit A (Sigma, clone 4F5) was used to determine the in situ localization of V-ATPases.

    Techniques: Immunostaining

    Intracellular localization of subunit a3 and markers for the Golgi apparatus and lysosomes in breast cancer cells MCF10a, MCF10CA1a, MB231, and SUM149 cells were grown on poly-d-lysine coated coverslips. To assess whether a3 localizes to the Golgi and lysosomes, cells were fixed, permeabilized, and immunostained using antibodies against subunit a3 of the V-ATPase and Giantin or LAMP2 as markers for the Golgi and lysosomes, respectively. Cells were then incubated with secondary antibodies as described under Materials and Methods. Images were taken with identical exposure times and antibody concentrations. A. Representative images of each of the cell lines stained for the Golgi apparatus marker Giantin ( left ), subunit a3 ( second from left ), the merged images ( second from right ), and the merged image magnified 3.5X ( labeled ‘Zoom’, far right ). B. Representative images of each of the cell lines stained for the lysosomal marker LAMP2 ( left ), subunit a3 ( second from left ), the merged images ( second from right ), and the inset of the merged image magnified 3.5X ( labeled ‘Zoom’, far right ). Colocalization is indicated by the presence of yellow pixels. The images in A and B depict representative staining from a minimum of two separate experiments, with an average of 50 cells imaged per experiment for each condition tested.

    Journal: Oncotarget

    Article Title: The a3 isoform of subunit a of the vacuolar ATPase localizes to the plasma membrane of invasive breast tumor cells and is overexpressed in human breast cancer

    doi: 10.18632/oncotarget.10063

    Figure Lengend Snippet: Intracellular localization of subunit a3 and markers for the Golgi apparatus and lysosomes in breast cancer cells MCF10a, MCF10CA1a, MB231, and SUM149 cells were grown on poly-d-lysine coated coverslips. To assess whether a3 localizes to the Golgi and lysosomes, cells were fixed, permeabilized, and immunostained using antibodies against subunit a3 of the V-ATPase and Giantin or LAMP2 as markers for the Golgi and lysosomes, respectively. Cells were then incubated with secondary antibodies as described under Materials and Methods. Images were taken with identical exposure times and antibody concentrations. A. Representative images of each of the cell lines stained for the Golgi apparatus marker Giantin ( left ), subunit a3 ( second from left ), the merged images ( second from right ), and the merged image magnified 3.5X ( labeled ‘Zoom’, far right ). B. Representative images of each of the cell lines stained for the lysosomal marker LAMP2 ( left ), subunit a3 ( second from left ), the merged images ( second from right ), and the inset of the merged image magnified 3.5X ( labeled ‘Zoom’, far right ). Colocalization is indicated by the presence of yellow pixels. The images in A and B depict representative staining from a minimum of two separate experiments, with an average of 50 cells imaged per experiment for each condition tested.

    Article Snippet: Briefly, an siRNA pool specific for the subunit a3 isoform of the V-ATPase was purchased from Dharmacon.

    Techniques: Incubation, Staining, Marker, Labeling

    Subunit a3 colocalizes with a leading edge marker in migrating breast cancer cells MCF10CA1a, MB231, and SUM149 breast cancer cells were grown to confluence on poly-d-lysine coated coverslips. A wound was made in the cell monolayer and cells were allowed to migrate for 4 h in order to induce the formation of a leading edge. Cells were then fixed, permeabilized, and immunostained using antibodies against subunit a3 of the V-ATPase and the leading edge marker cortactin followed by incubation with secondary antibodies as described under Materials and Methods. Images were taken with identical exposure times and antibody concentrations. Shown are representative images of MCF10CA1a, MB231, and SUM149 cells stained for cortactin ( left ), a3 ( second from left ), and DAPI ( second from right ). The merged images are present to the far right. White arrows indicate subunit a3 and cortactin colocalization at the leading edge. The images depict representative staining from a minimum of two separate experiments, with 50-80 cells imaged per experiment for each condition tested.

    Journal: Oncotarget

    Article Title: The a3 isoform of subunit a of the vacuolar ATPase localizes to the plasma membrane of invasive breast tumor cells and is overexpressed in human breast cancer

    doi: 10.18632/oncotarget.10063

    Figure Lengend Snippet: Subunit a3 colocalizes with a leading edge marker in migrating breast cancer cells MCF10CA1a, MB231, and SUM149 breast cancer cells were grown to confluence on poly-d-lysine coated coverslips. A wound was made in the cell monolayer and cells were allowed to migrate for 4 h in order to induce the formation of a leading edge. Cells were then fixed, permeabilized, and immunostained using antibodies against subunit a3 of the V-ATPase and the leading edge marker cortactin followed by incubation with secondary antibodies as described under Materials and Methods. Images were taken with identical exposure times and antibody concentrations. Shown are representative images of MCF10CA1a, MB231, and SUM149 cells stained for cortactin ( left ), a3 ( second from left ), and DAPI ( second from right ). The merged images are present to the far right. White arrows indicate subunit a3 and cortactin colocalization at the leading edge. The images depict representative staining from a minimum of two separate experiments, with 50-80 cells imaged per experiment for each condition tested.

    Article Snippet: Briefly, an siRNA pool specific for the subunit a3 isoform of the V-ATPase was purchased from Dharmacon.

    Techniques: Marker, Incubation, Staining

    Intracellular localization of subunit a3 and markers for early and late endosomes in breast cancer cells MCF10a, MCF10CA1a, MB231, and SUM149 cells were grown on poly-d-lysine coated coverslips. To assess whether a3 localizes to endosomal compartments, cells were fixed, permeabilized, and immunostained using antibodies against subunit a3 of the V-ATPase and EEA1 or Rab7 as markers for early endosomes and late endosomes, respectively. Cells were then incubated with secondary antibodies as described under Materials and Methods. Images were taken with identical exposure times and antibody concentrations. A. Representative images of each of the cell lines stained for the early endosome marker EEA1 ( left ), subunit a3 ( second from left ), the merged images ( second from right ), and the inset of the merged image magnified 3.5X ( labeled ‘Zoom’, far right ). B. Representative images of each of the cell lines stained for the late endosome marker Rab7 ( left ), subunit a3 ( second from left ), the merged images ( second from right ), and the merged image magnified 3.5X ( labeled ‘Zoom’, far right ). Colocalization is indicated by the presence of yellow pixels. The images in A and B depict representative staining from a minimum of two separate experiments, with an average of 50 cells imaged per experiment for each condition tested.

    Journal: Oncotarget

    Article Title: The a3 isoform of subunit a of the vacuolar ATPase localizes to the plasma membrane of invasive breast tumor cells and is overexpressed in human breast cancer

    doi: 10.18632/oncotarget.10063

    Figure Lengend Snippet: Intracellular localization of subunit a3 and markers for early and late endosomes in breast cancer cells MCF10a, MCF10CA1a, MB231, and SUM149 cells were grown on poly-d-lysine coated coverslips. To assess whether a3 localizes to endosomal compartments, cells were fixed, permeabilized, and immunostained using antibodies against subunit a3 of the V-ATPase and EEA1 or Rab7 as markers for early endosomes and late endosomes, respectively. Cells were then incubated with secondary antibodies as described under Materials and Methods. Images were taken with identical exposure times and antibody concentrations. A. Representative images of each of the cell lines stained for the early endosome marker EEA1 ( left ), subunit a3 ( second from left ), the merged images ( second from right ), and the inset of the merged image magnified 3.5X ( labeled ‘Zoom’, far right ). B. Representative images of each of the cell lines stained for the late endosome marker Rab7 ( left ), subunit a3 ( second from left ), the merged images ( second from right ), and the merged image magnified 3.5X ( labeled ‘Zoom’, far right ). Colocalization is indicated by the presence of yellow pixels. The images in A and B depict representative staining from a minimum of two separate experiments, with an average of 50 cells imaged per experiment for each condition tested.

    Article Snippet: Briefly, an siRNA pool specific for the subunit a3 isoform of the V-ATPase was purchased from Dharmacon.

    Techniques: Incubation, Staining, Marker, Labeling

    Subunit a3 localizes to the plasma membrane of human breast cancer cells MCF10a, MCF10CA1a, MB231, and SUM149 cells were grown to confluence on poly-d-lysine coated coverslips. To assess the effects of cell migration on a3 localization, a wound was made in the cell monolayer and cells were allowed to migrate for 4 h. Cells were then fixed, permeabilized, and immunostained using antibodies against subunit a3 of the V-ATPase and Alexa Fluor® 568 phalloidin to stain actin followed by incubation with secondary antibodies as described under Materials and Methods. Images were taken with identical exposure times and antibody concentrations. A. Shown are representative images of confluent MCF10a, MCF10CA1a, MB231, and SUM149 cells stained for phalloidin ( left ), subunit a3 (second from left ), DAPI ( second from right ), and the merged images ( right ). B. Shown are representative images of MCF10a, MCF10CA1a, MB231, and SUM149 cells (with monolayer wounded) stained for phalloidin ( left ), subunit a3 (second from left ), DAPI ( second from right ), and the merged images ( right ). White arrows indicate plasma membrane subunit a3. C. Quantification of plasma membrane staining for subunit a3 in confluent or migrating cells. To quantitate plasma membrane staining, 50-80 cells from three separate experiments were counted and the number of cells showing plasma membrane a3 localization was determined. The graphed data represents the percentage of cells showing a3 localization to the plasma membrane. All error bars indicate S.E. *, p

    Journal: Oncotarget

    Article Title: The a3 isoform of subunit a of the vacuolar ATPase localizes to the plasma membrane of invasive breast tumor cells and is overexpressed in human breast cancer

    doi: 10.18632/oncotarget.10063

    Figure Lengend Snippet: Subunit a3 localizes to the plasma membrane of human breast cancer cells MCF10a, MCF10CA1a, MB231, and SUM149 cells were grown to confluence on poly-d-lysine coated coverslips. To assess the effects of cell migration on a3 localization, a wound was made in the cell monolayer and cells were allowed to migrate for 4 h. Cells were then fixed, permeabilized, and immunostained using antibodies against subunit a3 of the V-ATPase and Alexa Fluor® 568 phalloidin to stain actin followed by incubation with secondary antibodies as described under Materials and Methods. Images were taken with identical exposure times and antibody concentrations. A. Shown are representative images of confluent MCF10a, MCF10CA1a, MB231, and SUM149 cells stained for phalloidin ( left ), subunit a3 (second from left ), DAPI ( second from right ), and the merged images ( right ). B. Shown are representative images of MCF10a, MCF10CA1a, MB231, and SUM149 cells (with monolayer wounded) stained for phalloidin ( left ), subunit a3 (second from left ), DAPI ( second from right ), and the merged images ( right ). White arrows indicate plasma membrane subunit a3. C. Quantification of plasma membrane staining for subunit a3 in confluent or migrating cells. To quantitate plasma membrane staining, 50-80 cells from three separate experiments were counted and the number of cells showing plasma membrane a3 localization was determined. The graphed data represents the percentage of cells showing a3 localization to the plasma membrane. All error bars indicate S.E. *, p

    Article Snippet: Briefly, an siRNA pool specific for the subunit a3 isoform of the V-ATPase was purchased from Dharmacon.

    Techniques: Migration, Staining, Incubation

    Colocalization of subunits of ENaC and Na + -, K + -ATPase with markers specific for TI and TII cells. The preparations of isolated cells used in these experiments contain not only TI cells and TII cells, but various other lung cells liberated by enzymatic digestion, including airway cells, macrophages, and cells from the vascular and interstitial compartments. Immunocytochemistry was performed as described in Methods . The same field of cells is shown in each row, stained with three antibodies and three different fluorophores specific for each antibody. The colors shown in each column are the result of scanning with the appropriate laser for each fluorophore. In the various columns, one can appreciate staining for ( a ) antibodies against various channel or pump proteins (blue color); ( b ) RTI40, a marker for TI cells (red color); and ( c ) RTII70, a marker for TII cells (green color). Column d shows a composite image, created by superimposition of the three separate images shown in columns a , b , and c . Arrows indicate one representative TI cell, one TII cell, and a cell that is neither a TI nor a TII cell. Every TI and TII cell expressed all of the pump/channel proteins. This fact can be appreciated most easily when one looks in column d at the spectral shifts caused by overlapping red (TI) or green (TII) markers with the blue (pump/channel) protein. In this composite image, colocalization of a transport protein subunit (ENaC or Na + -, K + -ATPase) with either TI or TII cell plasma membrane causes a color shift, indicating colocalization of the protein subunit with TI or TII plasma membranes. For example, combination of blue (subunit) with red (TI) is pinkish purple, whereas a combination of blue (subunit) with green (TII) is bluish green. Specific antibodies are labeled in rows 1–5: row 1, αENaC; row 2, βENaC; row 3, γENaC; row 4, α 1 -Na + -, K + -ATPase; row 5, β 1 -Na + -, K + -ATPase; row 6, Cos7 cells stained for αENaC; and row 7, no primary antibody control panel with mixed lung cells.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Alveolar epithelial type I cells contain transport proteins and transport sodium, supporting an active role for type I cells in regulation of lung liquid homeostasis

    doi: 10.1073/pnas.042689399

    Figure Lengend Snippet: Colocalization of subunits of ENaC and Na + -, K + -ATPase with markers specific for TI and TII cells. The preparations of isolated cells used in these experiments contain not only TI cells and TII cells, but various other lung cells liberated by enzymatic digestion, including airway cells, macrophages, and cells from the vascular and interstitial compartments. Immunocytochemistry was performed as described in Methods . The same field of cells is shown in each row, stained with three antibodies and three different fluorophores specific for each antibody. The colors shown in each column are the result of scanning with the appropriate laser for each fluorophore. In the various columns, one can appreciate staining for ( a ) antibodies against various channel or pump proteins (blue color); ( b ) RTI40, a marker for TI cells (red color); and ( c ) RTII70, a marker for TII cells (green color). Column d shows a composite image, created by superimposition of the three separate images shown in columns a , b , and c . Arrows indicate one representative TI cell, one TII cell, and a cell that is neither a TI nor a TII cell. Every TI and TII cell expressed all of the pump/channel proteins. This fact can be appreciated most easily when one looks in column d at the spectral shifts caused by overlapping red (TI) or green (TII) markers with the blue (pump/channel) protein. In this composite image, colocalization of a transport protein subunit (ENaC or Na + -, K + -ATPase) with either TI or TII cell plasma membrane causes a color shift, indicating colocalization of the protein subunit with TI or TII plasma membranes. For example, combination of blue (subunit) with red (TI) is pinkish purple, whereas a combination of blue (subunit) with green (TII) is bluish green. Specific antibodies are labeled in rows 1–5: row 1, αENaC; row 2, βENaC; row 3, γENaC; row 4, α 1 -Na + -, K + -ATPase; row 5, β 1 -Na + -, K + -ATPase; row 6, Cos7 cells stained for αENaC; and row 7, no primary antibody control panel with mixed lung cells.

    Article Snippet: Immunocytochemical studies were performed by using commercially available rabbit polyclonal antibodies to two subunits of Na+ -, K+ -ATPase (anti-Na+ -, K+ -ATPase α-1 and anti-Na+ -, K+ -ATPase β-1, Upstate Biotechnology, Lake Placid, NY).

    Techniques: Isolation, Immunocytochemistry, Staining, Marker, Labeling

    Expression of candidate cysteine transporters in rat heart. a ASCT1. The top panel shows the results of a representative RT-PCR. Lane 1 contains a ladder; lane 2 contains cDNA isolated from cardiomyocytes; lane 3 contains RNA isolated from cardiomyocytes; lane 4 contains cDNA isolated from brain; and lane 5 contains water. The middle panel shows Western blotting results. Lanes 1 and 2 contain brain synaptosomal membrane vesicles (BSMV); lanes 3 and 4 contain cardiac sarcolemmal vesicles (CSV). The bottom panel shows the same membrane following stripping and re-probing for the α 1 subunit of Na + , K + , ATPase. b ASCT2. The top panel shows the results of a representative RT-PCR. Lane 1 contains a ladder; lane 2 contains cDNA isolated from brain; lane 3 contains RNA isolated from cardiomyocytes; lane 4 contains water; lanes 5 and 6 contain cDNA isolated from cardiomyocytes. The middle panel shows Western blot results. Lane 1 contains a ladder; lane 2 contains BSMV; lane 3 contains CSV. The bottom panel shows the same membrane following stripping and re-probing for the α 1 subunit of Na + , K + , ATPase. Lanes 1 and 2 BSMV; lanes 3 and 4 CSV. c SNAT1. The top panel shows RT-PCR results. Lane 1 contains a ladder; lane 2 contains cDNA isolated from cardiomyocytes; lane 3 contains cDNA isolated from the brain. The middle panel shows Western blot results. Lane 1 contains a ladder; lane 2 contains CSV; lane 3 contains cardiomyocytes; lane 4 contains BSMV. The bottom panel shows the same membrane following stripping and re-probing for the α 1 subunit of Na + , K + , ATPase. Lane 1 contains CSV; lane 2 contains cardiomyocytes; lane 3 contains BSMV. d SNAT2. The top panel shows RT-PCR results. Lane 1 contains a ladder; lane 2 contains cDNA isolated from cardiomyocytes; lane 3 contains water; lane 4 contains cDNA isolated from the brain; lane 5 contains RNA isolated from cardiomyocytes. The middle panel shows Western blot results. Lane 1 contains a ladder; lane 2 contains L6 myotube cells; lane 3 contains BSMV; lane 4 contains cardiomyocytes; lane 5 contains CSV. The bottom panel shows the same membrane following stripping and re-probing for the α 1 subunit of Na + , K + , ATPase. All of the experiments were repeated three to five times with similar results

    Journal: Amino acids

    Article Title: Oxidative stress increases SNAT1 expression and stimulates cysteine uptake in freshly isolated rat cardiomyocytes

    doi: 10.1007/s00726-010-0664-6

    Figure Lengend Snippet: Expression of candidate cysteine transporters in rat heart. a ASCT1. The top panel shows the results of a representative RT-PCR. Lane 1 contains a ladder; lane 2 contains cDNA isolated from cardiomyocytes; lane 3 contains RNA isolated from cardiomyocytes; lane 4 contains cDNA isolated from brain; and lane 5 contains water. The middle panel shows Western blotting results. Lanes 1 and 2 contain brain synaptosomal membrane vesicles (BSMV); lanes 3 and 4 contain cardiac sarcolemmal vesicles (CSV). The bottom panel shows the same membrane following stripping and re-probing for the α 1 subunit of Na + , K + , ATPase. b ASCT2. The top panel shows the results of a representative RT-PCR. Lane 1 contains a ladder; lane 2 contains cDNA isolated from brain; lane 3 contains RNA isolated from cardiomyocytes; lane 4 contains water; lanes 5 and 6 contain cDNA isolated from cardiomyocytes. The middle panel shows Western blot results. Lane 1 contains a ladder; lane 2 contains BSMV; lane 3 contains CSV. The bottom panel shows the same membrane following stripping and re-probing for the α 1 subunit of Na + , K + , ATPase. Lanes 1 and 2 BSMV; lanes 3 and 4 CSV. c SNAT1. The top panel shows RT-PCR results. Lane 1 contains a ladder; lane 2 contains cDNA isolated from cardiomyocytes; lane 3 contains cDNA isolated from the brain. The middle panel shows Western blot results. Lane 1 contains a ladder; lane 2 contains CSV; lane 3 contains cardiomyocytes; lane 4 contains BSMV. The bottom panel shows the same membrane following stripping and re-probing for the α 1 subunit of Na + , K + , ATPase. Lane 1 contains CSV; lane 2 contains cardiomyocytes; lane 3 contains BSMV. d SNAT2. The top panel shows RT-PCR results. Lane 1 contains a ladder; lane 2 contains cDNA isolated from cardiomyocytes; lane 3 contains water; lane 4 contains cDNA isolated from the brain; lane 5 contains RNA isolated from cardiomyocytes. The middle panel shows Western blot results. Lane 1 contains a ladder; lane 2 contains L6 myotube cells; lane 3 contains BSMV; lane 4 contains cardiomyocytes; lane 5 contains CSV. The bottom panel shows the same membrane following stripping and re-probing for the α 1 subunit of Na + , K + , ATPase. All of the experiments were repeated three to five times with similar results

    Article Snippet: The quality of the protein loading in these experiments was tested by stripping the nitrocellulose membranes and re-probing with an antibody to the α 1-subunit of Na+ , K+ , ATPase (Abcam, Cambridge, UK).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Isolation, Western Blot, Stripping Membranes