atpase Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore adenosine triphosphatase atpase
    Adenosine Triphosphatase Atpase, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adenosine triphosphatase atpase/product/Millipore
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    adenosine triphosphatase atpase - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    88
    Agrisera vacuolor adenosine triphosphatase v atpase antibody
    Vacuolor Adenosine Triphosphatase V Atpase Antibody, supplied by Agrisera, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vacuolor adenosine triphosphatase v atpase antibody/product/Agrisera
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    vacuolor adenosine triphosphatase v atpase antibody - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    90
    Biomol GmbH atpase assay adenosine triphosphatase atpase activity
    Atpase Assay Adenosine Triphosphatase Atpase Activity, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atpase assay adenosine triphosphatase atpase activity/product/Biomol GmbH
    Average 90 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    atpase assay adenosine triphosphatase atpase activity - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    93
    Cell Signaling Technology Inc na k atpase
    Effect of garlic and its metabolites on Na + /K + <t>-ATPase</t> expression. (A,B) Western blot and bar graph of Na + /K + -ATPase from H9C2 cells. (C,D) Western blot and bar graph of Na + /K + -ATPase from rat heart. (Gels and blots were cropped and run under same experimental conditions). ( N = 3). Data were shown as mean ± SEM, ∗ p
    Na K Atpase, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 523 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/na k atpase/product/Cell Signaling Technology Inc
    Average 93 stars, based on 523 article reviews
    Price from $9.99 to $1999.99
    na k atpase - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    89
    Cytoskeleton Inc atpases
    Effect of garlic and its metabolites on Na + /K + <t>-ATPase</t> expression. (A,B) Western blot and bar graph of Na + /K + -ATPase from H9C2 cells. (C,D) Western blot and bar graph of Na + /K + -ATPase from rat heart. (Gels and blots were cropped and run under same experimental conditions). ( N = 3). Data were shown as mean ± SEM, ∗ p
    Atpases, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 89/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atpases/product/Cytoskeleton Inc
    Average 89 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    atpases - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

    90
    rdi research diagnostics atpase
    Cell fractionation of shortened MBP-GFP hybrid protein. Cells expressing MBP[SS128]-GFP were fractionated into inner and outer membranes as described in Materials and Methods. The immunoblot shown was decorated with anti-GFP antibody. MBP[SS128]-GFP migrated at a predicted molecular mass of 40 kDa and fractionated with the outer membrane. An apparent smaller-molecular-weight derivative of this protein was also found in the inner membrane fraction. Molecular weight (MW) standards are as shown. IM, inner membrane fraction; IM-W, KCl wash of inner membrane fraction; OM, outer membrane fraction; OM-W, KCl wash of outer membrane fraction. Although not shown, <t>OmpA</t> and <t>ATPase</t> were used to monitor fractionation of the outer membrane and inner membranes, respectively.
    Atpase, supplied by rdi research diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atpase/product/rdi research diagnostics
    Average 90 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    atpase - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    89
    SAS institute atpases
    Cell fractionation of shortened MBP-GFP hybrid protein. Cells expressing MBP[SS128]-GFP were fractionated into inner and outer membranes as described in Materials and Methods. The immunoblot shown was decorated with anti-GFP antibody. MBP[SS128]-GFP migrated at a predicted molecular mass of 40 kDa and fractionated with the outer membrane. An apparent smaller-molecular-weight derivative of this protein was also found in the inner membrane fraction. Molecular weight (MW) standards are as shown. IM, inner membrane fraction; IM-W, KCl wash of inner membrane fraction; OM, outer membrane fraction; OM-W, KCl wash of outer membrane fraction. Although not shown, <t>OmpA</t> and <t>ATPase</t> were used to monitor fractionation of the outer membrane and inner membranes, respectively.
    Atpases, supplied by SAS institute, used in various techniques. Bioz Stars score: 89/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atpases/product/SAS institute
    Average 89 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    atpases - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

    95
    Millipore atpase
    a) Effects of digitoxin on the level of ATF3, EGR1 protein. MDA-MB-453 cells were treated with 0, 0.1, 0.2 or 1 μ g/ml of digitoxin and after 1 and 24 h extracts were prepared and analyzed by Western blotting; an antibody to β-actin was used as a loading control. b) siRNA to ERK2. Cell were pretreated with control (nonsilencing) or MAPK1 (ERK2) siRNA for 24 h, exposed to digitoxin (0.4 μg/ml) for 24 h and percent inhibition of cell proliferation determined by the MTT assay. Percentages are normalized to DMSO. c) Inhibition of <t>Na+-K+-ATPase</t> activity in response to increasing concentrations of <t>paclitaxel</t> or digitoxin. The Na+-K+-ATPase assay was performed as described in Materials and Methods. The DMSO controls contained 3.3% DMSO. BARS: SD of triplicate assays.
    Atpase, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atpase/product/Millipore
    Average 95 stars, based on 50 article reviews
    Price from $9.99 to $1999.99
    atpase - by Bioz Stars, 2020-07
    95/100 stars
      Buy from Supplier

    92
    Upstate Biotechnology Inc k atpase
    a) Effects of digitoxin on the level of ATF3, EGR1 protein. MDA-MB-453 cells were treated with 0, 0.1, 0.2 or 1 μ g/ml of digitoxin and after 1 and 24 h extracts were prepared and analyzed by Western blotting; an antibody to β-actin was used as a loading control. b) siRNA to ERK2. Cell were pretreated with control (nonsilencing) or MAPK1 (ERK2) siRNA for 24 h, exposed to digitoxin (0.4 μg/ml) for 24 h and percent inhibition of cell proliferation determined by the MTT assay. Percentages are normalized to DMSO. c) Inhibition of <t>Na+-K+-ATPase</t> activity in response to increasing concentrations of <t>paclitaxel</t> or digitoxin. The Na+-K+-ATPase assay was performed as described in Materials and Methods. The DMSO controls contained 3.3% DMSO. BARS: SD of triplicate assays.
    K Atpase, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 92/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k atpase/product/Upstate Biotechnology Inc
    Average 92 stars, based on 42 article reviews
    Price from $9.99 to $1999.99
    k atpase - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    90
    Abcam atpase
    Expression of candidate cysteine transporters in rat heart. a ASCT1. The top panel shows the results of a representative RT-PCR. Lane 1 contains a ladder; lane 2 contains cDNA isolated from cardiomyocytes; lane 3 contains RNA isolated from cardiomyocytes; lane 4 contains cDNA isolated from brain; and lane 5 contains water. The middle panel shows Western blotting results. Lanes 1 and 2 contain brain synaptosomal membrane vesicles (BSMV); lanes 3 and 4 contain cardiac sarcolemmal vesicles (CSV). The bottom panel shows the same membrane following stripping and re-probing for the <t>α</t> 1 subunit of Na + , K + , <t>ATPase.</t> b ASCT2. The top panel shows the results of a representative RT-PCR. Lane 1 contains a ladder; lane 2 contains cDNA isolated from brain; lane 3 contains RNA isolated from cardiomyocytes; lane 4 contains water; lanes 5 and 6 contain cDNA isolated from cardiomyocytes. The middle panel shows Western blot results. Lane 1 contains a ladder; lane 2 contains BSMV; lane 3 contains CSV. The bottom panel shows the same membrane following stripping and re-probing for the α 1 subunit of Na + , K + , ATPase. Lanes 1 and 2 BSMV; lanes 3 and 4 CSV. c SNAT1. The top panel shows RT-PCR results. Lane 1 contains a ladder; lane 2 contains cDNA isolated from cardiomyocytes; lane 3 contains cDNA isolated from the brain. The middle panel shows Western blot results. Lane 1 contains a ladder; lane 2 contains CSV; lane 3 contains cardiomyocytes; lane 4 contains BSMV. The bottom panel shows the same membrane following stripping and re-probing for the α 1 subunit of Na + , K + , ATPase. Lane 1 contains CSV; lane 2 contains cardiomyocytes; lane 3 contains BSMV. d SNAT2. The top panel shows RT-PCR results. Lane 1 contains a ladder; lane 2 contains cDNA isolated from cardiomyocytes; lane 3 contains water; lane 4 contains cDNA isolated from the brain; lane 5 contains RNA isolated from cardiomyocytes. The middle panel shows Western blot results. Lane 1 contains a ladder; lane 2 contains L6 myotube cells; lane 3 contains BSMV; lane 4 contains cardiomyocytes; lane 5 contains CSV. The bottom panel shows the same membrane following stripping and re-probing for the α 1 subunit of Na + , K + , ATPase. All of the experiments were repeated three to five times with similar results
    Atpase, supplied by Abcam, used in various techniques. Bioz Stars score: 90/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atpase/product/Abcam
    Average 90 stars, based on 41 article reviews
    Price from $9.99 to $1999.99
    atpase - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    85
    Seca Inc cytoplasmic atpase
    Expression of candidate cysteine transporters in rat heart. a ASCT1. The top panel shows the results of a representative RT-PCR. Lane 1 contains a ladder; lane 2 contains cDNA isolated from cardiomyocytes; lane 3 contains RNA isolated from cardiomyocytes; lane 4 contains cDNA isolated from brain; and lane 5 contains water. The middle panel shows Western blotting results. Lanes 1 and 2 contain brain synaptosomal membrane vesicles (BSMV); lanes 3 and 4 contain cardiac sarcolemmal vesicles (CSV). The bottom panel shows the same membrane following stripping and re-probing for the <t>α</t> 1 subunit of Na + , K + , <t>ATPase.</t> b ASCT2. The top panel shows the results of a representative RT-PCR. Lane 1 contains a ladder; lane 2 contains cDNA isolated from brain; lane 3 contains RNA isolated from cardiomyocytes; lane 4 contains water; lanes 5 and 6 contain cDNA isolated from cardiomyocytes. The middle panel shows Western blot results. Lane 1 contains a ladder; lane 2 contains BSMV; lane 3 contains CSV. The bottom panel shows the same membrane following stripping and re-probing for the α 1 subunit of Na + , K + , ATPase. Lanes 1 and 2 BSMV; lanes 3 and 4 CSV. c SNAT1. The top panel shows RT-PCR results. Lane 1 contains a ladder; lane 2 contains cDNA isolated from cardiomyocytes; lane 3 contains cDNA isolated from the brain. The middle panel shows Western blot results. Lane 1 contains a ladder; lane 2 contains CSV; lane 3 contains cardiomyocytes; lane 4 contains BSMV. The bottom panel shows the same membrane following stripping and re-probing for the α 1 subunit of Na + , K + , ATPase. Lane 1 contains CSV; lane 2 contains cardiomyocytes; lane 3 contains BSMV. d SNAT2. The top panel shows RT-PCR results. Lane 1 contains a ladder; lane 2 contains cDNA isolated from cardiomyocytes; lane 3 contains water; lane 4 contains cDNA isolated from the brain; lane 5 contains RNA isolated from cardiomyocytes. The middle panel shows Western blot results. Lane 1 contains a ladder; lane 2 contains L6 myotube cells; lane 3 contains BSMV; lane 4 contains cardiomyocytes; lane 5 contains CSV. The bottom panel shows the same membrane following stripping and re-probing for the α 1 subunit of Na + , K + , ATPase. All of the experiments were repeated three to five times with similar results
    Cytoplasmic Atpase, supplied by Seca Inc, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cytoplasmic atpase/product/Seca Inc
    Average 85 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    cytoplasmic atpase - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    93
    Santa Cruz Biotechnology atpase alpha3
    Expression of candidate cysteine transporters in rat heart. a ASCT1. The top panel shows the results of a representative RT-PCR. Lane 1 contains a ladder; lane 2 contains cDNA isolated from cardiomyocytes; lane 3 contains RNA isolated from cardiomyocytes; lane 4 contains cDNA isolated from brain; and lane 5 contains water. The middle panel shows Western blotting results. Lanes 1 and 2 contain brain synaptosomal membrane vesicles (BSMV); lanes 3 and 4 contain cardiac sarcolemmal vesicles (CSV). The bottom panel shows the same membrane following stripping and re-probing for the <t>α</t> 1 subunit of Na + , K + , <t>ATPase.</t> b ASCT2. The top panel shows the results of a representative RT-PCR. Lane 1 contains a ladder; lane 2 contains cDNA isolated from brain; lane 3 contains RNA isolated from cardiomyocytes; lane 4 contains water; lanes 5 and 6 contain cDNA isolated from cardiomyocytes. The middle panel shows Western blot results. Lane 1 contains a ladder; lane 2 contains BSMV; lane 3 contains CSV. The bottom panel shows the same membrane following stripping and re-probing for the α 1 subunit of Na + , K + , ATPase. Lanes 1 and 2 BSMV; lanes 3 and 4 CSV. c SNAT1. The top panel shows RT-PCR results. Lane 1 contains a ladder; lane 2 contains cDNA isolated from cardiomyocytes; lane 3 contains cDNA isolated from the brain. The middle panel shows Western blot results. Lane 1 contains a ladder; lane 2 contains CSV; lane 3 contains cardiomyocytes; lane 4 contains BSMV. The bottom panel shows the same membrane following stripping and re-probing for the α 1 subunit of Na + , K + , ATPase. Lane 1 contains CSV; lane 2 contains cardiomyocytes; lane 3 contains BSMV. d SNAT2. The top panel shows RT-PCR results. Lane 1 contains a ladder; lane 2 contains cDNA isolated from cardiomyocytes; lane 3 contains water; lane 4 contains cDNA isolated from the brain; lane 5 contains RNA isolated from cardiomyocytes. The middle panel shows Western blot results. Lane 1 contains a ladder; lane 2 contains L6 myotube cells; lane 3 contains BSMV; lane 4 contains cardiomyocytes; lane 5 contains CSV. The bottom panel shows the same membrane following stripping and re-probing for the α 1 subunit of Na + , K + , ATPase. All of the experiments were repeated three to five times with similar results
    Atpase Alpha3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atpase alpha3/product/Santa Cruz Biotechnology
    Average 93 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    atpase alpha3 - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    85
    Trinity Biotech atpase p97
    Expression of candidate cysteine transporters in rat heart. a ASCT1. The top panel shows the results of a representative RT-PCR. Lane 1 contains a ladder; lane 2 contains cDNA isolated from cardiomyocytes; lane 3 contains RNA isolated from cardiomyocytes; lane 4 contains cDNA isolated from brain; and lane 5 contains water. The middle panel shows Western blotting results. Lanes 1 and 2 contain brain synaptosomal membrane vesicles (BSMV); lanes 3 and 4 contain cardiac sarcolemmal vesicles (CSV). The bottom panel shows the same membrane following stripping and re-probing for the <t>α</t> 1 subunit of Na + , K + , <t>ATPase.</t> b ASCT2. The top panel shows the results of a representative RT-PCR. Lane 1 contains a ladder; lane 2 contains cDNA isolated from brain; lane 3 contains RNA isolated from cardiomyocytes; lane 4 contains water; lanes 5 and 6 contain cDNA isolated from cardiomyocytes. The middle panel shows Western blot results. Lane 1 contains a ladder; lane 2 contains BSMV; lane 3 contains CSV. The bottom panel shows the same membrane following stripping and re-probing for the α 1 subunit of Na + , K + , ATPase. Lanes 1 and 2 BSMV; lanes 3 and 4 CSV. c SNAT1. The top panel shows RT-PCR results. Lane 1 contains a ladder; lane 2 contains cDNA isolated from cardiomyocytes; lane 3 contains cDNA isolated from the brain. The middle panel shows Western blot results. Lane 1 contains a ladder; lane 2 contains CSV; lane 3 contains cardiomyocytes; lane 4 contains BSMV. The bottom panel shows the same membrane following stripping and re-probing for the α 1 subunit of Na + , K + , ATPase. Lane 1 contains CSV; lane 2 contains cardiomyocytes; lane 3 contains BSMV. d SNAT2. The top panel shows RT-PCR results. Lane 1 contains a ladder; lane 2 contains cDNA isolated from cardiomyocytes; lane 3 contains water; lane 4 contains cDNA isolated from the brain; lane 5 contains RNA isolated from cardiomyocytes. The middle panel shows Western blot results. Lane 1 contains a ladder; lane 2 contains L6 myotube cells; lane 3 contains BSMV; lane 4 contains cardiomyocytes; lane 5 contains CSV. The bottom panel shows the same membrane following stripping and re-probing for the α 1 subunit of Na + , K + , ATPase. All of the experiments were repeated three to five times with similar results
    Atpase P97, supplied by Trinity Biotech, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atpase p97/product/Trinity Biotech
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    atpase p97 - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    92
    Epitomics k atpase
    Models of ion transport in hRPTCs. This is a model of the hRPTC with the apical (brush border, facing the lumen) (left hand side) and the basolateral side (right hand side). The principal ion transporters and some of the receptors that regulate them are shown. Starting at 11 o’clock in blue is shown the classic pathway for transporting bicarbonate (HCO 3 - ) into the cell. Filtered NaHCO 3 dissociates into Na + and HCO 3 . HCO 3 - in the luminal fluid and H + secreted into the lumen form H 2 CO 3 . Carbonic anhydrase type 4 (CA IV) in the luminal membrane catalyzes the conversion of H 2 CO 3 to H 2 O and CO 2 .CO 2 diffuses inside the hRPTC where intracellular carbonic anhydrase type 2 (CA II) catalyzes the conversion of CO 2 and H 2 O into H 2 CO 3 which then dissociates into HCO 3 - and H + . At 9 o’clock is <t>NHE3</t> which exchanges one Na + from the lumen with one H + inside the hRPTC. At 7 o’clock HCO 3 - Cl - exchanger (PAT1) is depicted which exchanges luminal Cl - with cytoplasmic HCO 3 - . At 3 o’clock is depicted NBCe1 at the basolateral membrane which electrogenically transports 2–3 Na + and one HCO 3 - into the basolateral space. At 4 o’clock is Na + , K + <t>/ATPase</t> which pumps 3 Na + out of the cell into the blood stream and pumps in 2 K + inside the cell The topic of this manuscript deals with NBCe2, drawn at 8 o’clock. Under a normal sodium load it plays a minor role in Na + and HCO 3 - transport into the hRPTC. There are various plasma membrane receptors that regulate some of these transporters/ exchanger/pumps. The dopamine-1 receptor (D 1 R) (ten o’clock) when stimulated with dopamine (green box) inhibits (red lines) both NHE3 and Na + , K + /ATPase (without the red line, for simplicity) activities resulting in reduced Na + reabsorption and increased Na + excretion. The AT 1 R (5 o’clock) increases Na + , K + /ATPase activity (green arrow) resulting in increased Na + reabsorption. An increase in intracellular Na + increases Na + , K + /ATPase activity (5 o’clock) that is abetted by AT 1 R (green arrow) resulting in increased Na + transport from inside the cell to the basolateral space. The D 1 R and AT 1 R oppose each other. The D 1 R inhibits the AT 1 R, resulting in reduced Na + transport. We showed that NBCe2 is not affected by stimulation of the D 1 R or AT 1 R. Under basal conditions, NBCe1 is more active than NBCe2 (depicted as relatively larger directional transport arrows).
    K Atpase, supplied by Epitomics, used in various techniques. Bioz Stars score: 92/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k atpase/product/Epitomics
    Average 92 stars, based on 54 article reviews
    Price from $9.99 to $1999.99
    k atpase - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    92
    Millipore k atpase
    Effect of vanadate on renal <t>α</t> 1 -Na, <t>K-ATPase</t> protein abundance. Ten days of vanadate injection significantly suppressed α 1 -Na, K-ATPase protein abundance. Histogram bars show the densitometric analyses ratios of α 1 -Na, K-ATPase to β-actin intensity, and the representative immunoblot photographs are present. All values are expressed as the mean ± SD of 8 rats/group. *Significantly different from the sham group ( p
    K Atpase, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k atpase/product/Millipore
    Average 92 stars, based on 83 article reviews
    Price from $9.99 to $1999.99
    k atpase - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    90
    Horizon Discovery v atpase
    Intracellular localization of subunit <t>a3</t> and markers for the Golgi apparatus and lysosomes in breast cancer cells MCF10a, MCF10CA1a, MB231, and SUM149 cells were grown on poly-d-lysine coated coverslips. To assess whether a3 localizes to the Golgi and lysosomes, cells were fixed, permeabilized, and immunostained using antibodies against subunit a3 of the <t>V-ATPase</t> and Giantin or LAMP2 as markers for the Golgi and lysosomes, respectively. Cells were then incubated with secondary antibodies as described under Materials and Methods. Images were taken with identical exposure times and antibody concentrations. A. Representative images of each of the cell lines stained for the Golgi apparatus marker Giantin ( left ), subunit a3 ( second from left ), the merged images ( second from right ), and the merged image magnified 3.5X ( labeled ‘Zoom’, far right ). B. Representative images of each of the cell lines stained for the lysosomal marker LAMP2 ( left ), subunit a3 ( second from left ), the merged images ( second from right ), and the inset of the merged image magnified 3.5X ( labeled ‘Zoom’, far right ). Colocalization is indicated by the presence of yellow pixels. The images in A and B depict representative staining from a minimum of two separate experiments, with an average of 50 cells imaged per experiment for each condition tested.
    V Atpase, supplied by Horizon Discovery, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/v atpase/product/Horizon Discovery
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    v atpase - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    91
    Santa Cruz Biotechnology v atpase
    ( A ) Triple immunofluorescence labeling of a kidney section from AQP2-cre + tdTomato + (PC reporter) mice showing: AQP2 (red cells, upper left);. <t>V-ATPase</t> E1 (green cells, ICs, upper right); tdTomato (blue cells, lower left) staining in inner medulla. On the merged image (lower right) most AQP2 cells were also positive for tdTomato (purple cells, white arrows) while some were negative for tdTomato (red cells, white arrowheads). The dashed box contains a tubule imaged from another kidney section that contained an isolated cell that was positive for both AQP2 and V-ATPAse E1 (Yellow cell, asterisk). Magnification 40X, confocal microscopy. ( B ) Triple immunofluorescence labeling of a kidney section from V-ATPase <t>B1-cre</t> + tdTomato + (IC reporter) mice showing: V-ATPase B1 (red cells, upper left). tdTomato (green cells, upper right) and nuclei (blue, lower left) staining in inner medulla. On the merged image (lower right) most V-ATPase B1 positive cells were also tdTomato positive (yellow cells, white arrows) while a few cells were tdTomato positive but were V-ATPase B1 negative (green cells, white arrowheads). Magnification 20X, immunofluorescent microscopy.
    V Atpase, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/v atpase/product/Santa Cruz Biotechnology
    Average 91 stars, based on 131 article reviews
    Price from $9.99 to $1999.99
    v atpase - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    92
    Thermo Fisher k atpase
    STED microscopy dissecting the localization of Na + ,K + <t>-ATPase</t> in cultured striatal neurons . The 5 × 3 mosaic shows a comparision of the confocal (left) and STED images (middle) of the Alexa-594 immunolabelled <t>α3</t> NKA in dendritic spines. Postprocessing of the raw STED data by a Richardson-Lucy deconvolution further enhances the details as shown in the right row of images. Scale bar: 500 nm
    K Atpase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k atpase/product/Thermo Fisher
    Average 92 stars, based on 38 article reviews
    Price from $9.99 to $1999.99
    k atpase - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    91
    Synaptic Systems v atpase
    STED microscopy dissecting the localization of Na + ,K + <t>-ATPase</t> in cultured striatal neurons . The 5 × 3 mosaic shows a comparision of the confocal (left) and STED images (middle) of the Alexa-594 immunolabelled <t>α3</t> NKA in dendritic spines. Postprocessing of the raw STED data by a Richardson-Lucy deconvolution further enhances the details as shown in the right row of images. Scale bar: 500 nm
    V Atpase, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 91/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/v atpase/product/Synaptic Systems
    Average 91 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    v atpase - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    92
    Tocris atpase inhibitor
    STED microscopy dissecting the localization of Na + ,K + <t>-ATPase</t> in cultured striatal neurons . The 5 × 3 mosaic shows a comparision of the confocal (left) and STED images (middle) of the Alexa-594 immunolabelled <t>α3</t> NKA in dendritic spines. Postprocessing of the raw STED data by a Richardson-Lucy deconvolution further enhances the details as shown in the right row of images. Scale bar: 500 nm
    Atpase Inhibitor, supplied by Tocris, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atpase inhibitor/product/Tocris
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    atpase inhibitor - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    85
    Seca Inc atpase seca
    STED microscopy dissecting the localization of Na + ,K + <t>-ATPase</t> in cultured striatal neurons . The 5 × 3 mosaic shows a comparision of the confocal (left) and STED images (middle) of the Alexa-594 immunolabelled <t>α3</t> NKA in dendritic spines. Postprocessing of the raw STED data by a Richardson-Lucy deconvolution further enhances the details as shown in the right row of images. Scale bar: 500 nm
    Atpase Seca, supplied by Seca Inc, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atpase seca/product/Seca Inc
    Average 85 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    atpase seca - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    92
    Developmental Studies Hybridoma Bank k atpase
    The immunoblots and relative intensities of Prdx6 in membrane-fraction proteins of livers of the fresh water (FW) and seawater (SW) milkfish acclimated to 28°C (white bar) and 18°C (stripe bar) for 1 week, respectively . In the representative immunoblot, single immunoreactive bands at 100 and 25 kDa represent Na + , K + <t>-ATPase</t> <t>(NKA),</t> and Prdx6, respectively. The NKA was used as the loading control of the membrane fraction. Different letters (a and b) indicate significant differences between the 28° and 18°C group, and (x and y) indicate significant differences between the FW and SW group. Values are means ± SEM, n = 6. The Student's t -test pairwise comparison was used following two-way ANOVA, P
    K Atpase, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 92/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k atpase/product/Developmental Studies Hybridoma Bank
    Average 92 stars, based on 61 article reviews
    Price from $9.99 to $1999.99
    k atpase - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    85
    MitoSciences mitochondrial atpase
    The immunoblots and relative intensities of Prdx6 in membrane-fraction proteins of livers of the fresh water (FW) and seawater (SW) milkfish acclimated to 28°C (white bar) and 18°C (stripe bar) for 1 week, respectively . In the representative immunoblot, single immunoreactive bands at 100 and 25 kDa represent Na + , K + <t>-ATPase</t> <t>(NKA),</t> and Prdx6, respectively. The NKA was used as the loading control of the membrane fraction. Different letters (a and b) indicate significant differences between the 28° and 18°C group, and (x and y) indicate significant differences between the FW and SW group. Values are means ± SEM, n = 6. The Student's t -test pairwise comparison was used following two-way ANOVA, P
    Mitochondrial Atpase, supplied by MitoSciences, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mitochondrial atpase/product/MitoSciences
    Average 85 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    mitochondrial atpase - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    92
    MBL International k atpase
    The immunoblots and relative intensities of Prdx6 in membrane-fraction proteins of livers of the fresh water (FW) and seawater (SW) milkfish acclimated to 28°C (white bar) and 18°C (stripe bar) for 1 week, respectively . In the representative immunoblot, single immunoreactive bands at 100 and 25 kDa represent Na + , K + <t>-ATPase</t> <t>(NKA),</t> and Prdx6, respectively. The NKA was used as the loading control of the membrane fraction. Different letters (a and b) indicate significant differences between the 28° and 18°C group, and (x and y) indicate significant differences between the FW and SW group. Values are means ± SEM, n = 6. The Student's t -test pairwise comparison was used following two-way ANOVA, P
    K Atpase, supplied by MBL International, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/k atpase/product/MBL International
    Average 92 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    k atpase - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    Image Search Results


    Effect of garlic and its metabolites on Na + /K + -ATPase expression. (A,B) Western blot and bar graph of Na + /K + -ATPase from H9C2 cells. (C,D) Western blot and bar graph of Na + /K + -ATPase from rat heart. (Gels and blots were cropped and run under same experimental conditions). ( N = 3). Data were shown as mean ± SEM, ∗ p

    Journal: Frontiers in Pharmacology

    Article Title: Novel Sulfur Metabolites of Garlic Attenuate Cardiac Hypertrophy and Remodeling through Induction of Na+/K+-ATPase Expression

    doi: 10.3389/fphar.2017.00018

    Figure Lengend Snippet: Effect of garlic and its metabolites on Na + /K + -ATPase expression. (A,B) Western blot and bar graph of Na + /K + -ATPase from H9C2 cells. (C,D) Western blot and bar graph of Na + /K + -ATPase from rat heart. (Gels and blots were cropped and run under same experimental conditions). ( N = 3). Data were shown as mean ± SEM, ∗ p

    Article Snippet: Garlic and Its Metabolites Failed to Show Any Anti-Hypertrophic Effect in H9C2 Cells in Presence of Na+ /K+ -ATPase Inhibitor To confirm whether garlic and its metabolites showed their anti-hypertrophic effect through Na+ /K+ -ATPase, we treated H9C2 cells with Iso in presence and absence of digoxin, a Na+ /K+ -ATPase inhibitor.

    Techniques: Expressing, Western Blot

    Cell fractionation of shortened MBP-GFP hybrid protein. Cells expressing MBP[SS128]-GFP were fractionated into inner and outer membranes as described in Materials and Methods. The immunoblot shown was decorated with anti-GFP antibody. MBP[SS128]-GFP migrated at a predicted molecular mass of 40 kDa and fractionated with the outer membrane. An apparent smaller-molecular-weight derivative of this protein was also found in the inner membrane fraction. Molecular weight (MW) standards are as shown. IM, inner membrane fraction; IM-W, KCl wash of inner membrane fraction; OM, outer membrane fraction; OM-W, KCl wash of outer membrane fraction. Although not shown, OmpA and ATPase were used to monitor fractionation of the outer membrane and inner membranes, respectively.

    Journal: Journal of Bacteriology

    Article Title: Green Fluorescent Protein Functions as a Reporter for Protein Localization in Escherichia coli †

    doi:

    Figure Lengend Snippet: Cell fractionation of shortened MBP-GFP hybrid protein. Cells expressing MBP[SS128]-GFP were fractionated into inner and outer membranes as described in Materials and Methods. The immunoblot shown was decorated with anti-GFP antibody. MBP[SS128]-GFP migrated at a predicted molecular mass of 40 kDa and fractionated with the outer membrane. An apparent smaller-molecular-weight derivative of this protein was also found in the inner membrane fraction. Molecular weight (MW) standards are as shown. IM, inner membrane fraction; IM-W, KCl wash of inner membrane fraction; OM, outer membrane fraction; OM-W, KCl wash of outer membrane fraction. Although not shown, OmpA and ATPase were used to monitor fractionation of the outer membrane and inner membranes, respectively.

    Article Snippet: Antibodies to OmpA and ATPase (Research Diagnostics, Flanders, N.J.) were used to monitor fractionation of the outer and inner membranes, respectively.

    Techniques: Cell Fractionation, Expressing, Molecular Weight, Fractionation

    a) Effects of digitoxin on the level of ATF3, EGR1 protein. MDA-MB-453 cells were treated with 0, 0.1, 0.2 or 1 μ g/ml of digitoxin and after 1 and 24 h extracts were prepared and analyzed by Western blotting; an antibody to β-actin was used as a loading control. b) siRNA to ERK2. Cell were pretreated with control (nonsilencing) or MAPK1 (ERK2) siRNA for 24 h, exposed to digitoxin (0.4 μg/ml) for 24 h and percent inhibition of cell proliferation determined by the MTT assay. Percentages are normalized to DMSO. c) Inhibition of Na+-K+-ATPase activity in response to increasing concentrations of paclitaxel or digitoxin. The Na+-K+-ATPase assay was performed as described in Materials and Methods. The DMSO controls contained 3.3% DMSO. BARS: SD of triplicate assays.

    Journal: Journal of Carcinogenesis

    Article Title: Digitoxin activates EGR1 and synergizes with paclitaxel on human breast cancer cells

    doi: 10.4103/1477-3163.72578

    Figure Lengend Snippet: a) Effects of digitoxin on the level of ATF3, EGR1 protein. MDA-MB-453 cells were treated with 0, 0.1, 0.2 or 1 μ g/ml of digitoxin and after 1 and 24 h extracts were prepared and analyzed by Western blotting; an antibody to β-actin was used as a loading control. b) siRNA to ERK2. Cell were pretreated with control (nonsilencing) or MAPK1 (ERK2) siRNA for 24 h, exposed to digitoxin (0.4 μg/ml) for 24 h and percent inhibition of cell proliferation determined by the MTT assay. Percentages are normalized to DMSO. c) Inhibition of Na+-K+-ATPase activity in response to increasing concentrations of paclitaxel or digitoxin. The Na+-K+-ATPase assay was performed as described in Materials and Methods. The DMSO controls contained 3.3% DMSO. BARS: SD of triplicate assays.

    Article Snippet: Digitoxin or paclitaxel was added to ATPase (0.05 ml, 0.5 unit/ml, Sigma-Aldrich, St. Louis, MO, USA), mixed and equilibrated for 5 min at 37°C.

    Techniques: Multiple Displacement Amplification, Western Blot, Inhibition, MTT Assay, Activity Assay, ATPase Assay

    Expression of candidate cysteine transporters in rat heart. a ASCT1. The top panel shows the results of a representative RT-PCR. Lane 1 contains a ladder; lane 2 contains cDNA isolated from cardiomyocytes; lane 3 contains RNA isolated from cardiomyocytes; lane 4 contains cDNA isolated from brain; and lane 5 contains water. The middle panel shows Western blotting results. Lanes 1 and 2 contain brain synaptosomal membrane vesicles (BSMV); lanes 3 and 4 contain cardiac sarcolemmal vesicles (CSV). The bottom panel shows the same membrane following stripping and re-probing for the α 1 subunit of Na + , K + , ATPase. b ASCT2. The top panel shows the results of a representative RT-PCR. Lane 1 contains a ladder; lane 2 contains cDNA isolated from brain; lane 3 contains RNA isolated from cardiomyocytes; lane 4 contains water; lanes 5 and 6 contain cDNA isolated from cardiomyocytes. The middle panel shows Western blot results. Lane 1 contains a ladder; lane 2 contains BSMV; lane 3 contains CSV. The bottom panel shows the same membrane following stripping and re-probing for the α 1 subunit of Na + , K + , ATPase. Lanes 1 and 2 BSMV; lanes 3 and 4 CSV. c SNAT1. The top panel shows RT-PCR results. Lane 1 contains a ladder; lane 2 contains cDNA isolated from cardiomyocytes; lane 3 contains cDNA isolated from the brain. The middle panel shows Western blot results. Lane 1 contains a ladder; lane 2 contains CSV; lane 3 contains cardiomyocytes; lane 4 contains BSMV. The bottom panel shows the same membrane following stripping and re-probing for the α 1 subunit of Na + , K + , ATPase. Lane 1 contains CSV; lane 2 contains cardiomyocytes; lane 3 contains BSMV. d SNAT2. The top panel shows RT-PCR results. Lane 1 contains a ladder; lane 2 contains cDNA isolated from cardiomyocytes; lane 3 contains water; lane 4 contains cDNA isolated from the brain; lane 5 contains RNA isolated from cardiomyocytes. The middle panel shows Western blot results. Lane 1 contains a ladder; lane 2 contains L6 myotube cells; lane 3 contains BSMV; lane 4 contains cardiomyocytes; lane 5 contains CSV. The bottom panel shows the same membrane following stripping and re-probing for the α 1 subunit of Na + , K + , ATPase. All of the experiments were repeated three to five times with similar results

    Journal: Amino acids

    Article Title: Oxidative stress increases SNAT1 expression and stimulates cysteine uptake in freshly isolated rat cardiomyocytes

    doi: 10.1007/s00726-010-0664-6

    Figure Lengend Snippet: Expression of candidate cysteine transporters in rat heart. a ASCT1. The top panel shows the results of a representative RT-PCR. Lane 1 contains a ladder; lane 2 contains cDNA isolated from cardiomyocytes; lane 3 contains RNA isolated from cardiomyocytes; lane 4 contains cDNA isolated from brain; and lane 5 contains water. The middle panel shows Western blotting results. Lanes 1 and 2 contain brain synaptosomal membrane vesicles (BSMV); lanes 3 and 4 contain cardiac sarcolemmal vesicles (CSV). The bottom panel shows the same membrane following stripping and re-probing for the α 1 subunit of Na + , K + , ATPase. b ASCT2. The top panel shows the results of a representative RT-PCR. Lane 1 contains a ladder; lane 2 contains cDNA isolated from brain; lane 3 contains RNA isolated from cardiomyocytes; lane 4 contains water; lanes 5 and 6 contain cDNA isolated from cardiomyocytes. The middle panel shows Western blot results. Lane 1 contains a ladder; lane 2 contains BSMV; lane 3 contains CSV. The bottom panel shows the same membrane following stripping and re-probing for the α 1 subunit of Na + , K + , ATPase. Lanes 1 and 2 BSMV; lanes 3 and 4 CSV. c SNAT1. The top panel shows RT-PCR results. Lane 1 contains a ladder; lane 2 contains cDNA isolated from cardiomyocytes; lane 3 contains cDNA isolated from the brain. The middle panel shows Western blot results. Lane 1 contains a ladder; lane 2 contains CSV; lane 3 contains cardiomyocytes; lane 4 contains BSMV. The bottom panel shows the same membrane following stripping and re-probing for the α 1 subunit of Na + , K + , ATPase. Lane 1 contains CSV; lane 2 contains cardiomyocytes; lane 3 contains BSMV. d SNAT2. The top panel shows RT-PCR results. Lane 1 contains a ladder; lane 2 contains cDNA isolated from cardiomyocytes; lane 3 contains water; lane 4 contains cDNA isolated from the brain; lane 5 contains RNA isolated from cardiomyocytes. The middle panel shows Western blot results. Lane 1 contains a ladder; lane 2 contains L6 myotube cells; lane 3 contains BSMV; lane 4 contains cardiomyocytes; lane 5 contains CSV. The bottom panel shows the same membrane following stripping and re-probing for the α 1 subunit of Na + , K + , ATPase. All of the experiments were repeated three to five times with similar results

    Article Snippet: The quality of the protein loading in these experiments was tested by stripping the nitrocellulose membranes and re-probing with an antibody to the α 1-subunit of Na+ , K+ , ATPase (Abcam, Cambridge, UK).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Isolation, Western Blot, Stripping Membranes

    Models of ion transport in hRPTCs. This is a model of the hRPTC with the apical (brush border, facing the lumen) (left hand side) and the basolateral side (right hand side). The principal ion transporters and some of the receptors that regulate them are shown. Starting at 11 o’clock in blue is shown the classic pathway for transporting bicarbonate (HCO 3 - ) into the cell. Filtered NaHCO 3 dissociates into Na + and HCO 3 . HCO 3 - in the luminal fluid and H + secreted into the lumen form H 2 CO 3 . Carbonic anhydrase type 4 (CA IV) in the luminal membrane catalyzes the conversion of H 2 CO 3 to H 2 O and CO 2 .CO 2 diffuses inside the hRPTC where intracellular carbonic anhydrase type 2 (CA II) catalyzes the conversion of CO 2 and H 2 O into H 2 CO 3 which then dissociates into HCO 3 - and H + . At 9 o’clock is NHE3 which exchanges one Na + from the lumen with one H + inside the hRPTC. At 7 o’clock HCO 3 - Cl - exchanger (PAT1) is depicted which exchanges luminal Cl - with cytoplasmic HCO 3 - . At 3 o’clock is depicted NBCe1 at the basolateral membrane which electrogenically transports 2–3 Na + and one HCO 3 - into the basolateral space. At 4 o’clock is Na + , K + /ATPase which pumps 3 Na + out of the cell into the blood stream and pumps in 2 K + inside the cell The topic of this manuscript deals with NBCe2, drawn at 8 o’clock. Under a normal sodium load it plays a minor role in Na + and HCO 3 - transport into the hRPTC. There are various plasma membrane receptors that regulate some of these transporters/ exchanger/pumps. The dopamine-1 receptor (D 1 R) (ten o’clock) when stimulated with dopamine (green box) inhibits (red lines) both NHE3 and Na + , K + /ATPase (without the red line, for simplicity) activities resulting in reduced Na + reabsorption and increased Na + excretion. The AT 1 R (5 o’clock) increases Na + , K + /ATPase activity (green arrow) resulting in increased Na + reabsorption. An increase in intracellular Na + increases Na + , K + /ATPase activity (5 o’clock) that is abetted by AT 1 R (green arrow) resulting in increased Na + transport from inside the cell to the basolateral space. The D 1 R and AT 1 R oppose each other. The D 1 R inhibits the AT 1 R, resulting in reduced Na + transport. We showed that NBCe2 is not affected by stimulation of the D 1 R or AT 1 R. Under basal conditions, NBCe1 is more active than NBCe2 (depicted as relatively larger directional transport arrows).

    Journal: PLoS ONE

    Article Title: Sodium bicarbonate cotransporter NBCe2 gene variants increase sodium and bicarbonate transport in human renal proximal tubule cells

    doi: 10.1371/journal.pone.0189464

    Figure Lengend Snippet: Models of ion transport in hRPTCs. This is a model of the hRPTC with the apical (brush border, facing the lumen) (left hand side) and the basolateral side (right hand side). The principal ion transporters and some of the receptors that regulate them are shown. Starting at 11 o’clock in blue is shown the classic pathway for transporting bicarbonate (HCO 3 - ) into the cell. Filtered NaHCO 3 dissociates into Na + and HCO 3 . HCO 3 - in the luminal fluid and H + secreted into the lumen form H 2 CO 3 . Carbonic anhydrase type 4 (CA IV) in the luminal membrane catalyzes the conversion of H 2 CO 3 to H 2 O and CO 2 .CO 2 diffuses inside the hRPTC where intracellular carbonic anhydrase type 2 (CA II) catalyzes the conversion of CO 2 and H 2 O into H 2 CO 3 which then dissociates into HCO 3 - and H + . At 9 o’clock is NHE3 which exchanges one Na + from the lumen with one H + inside the hRPTC. At 7 o’clock HCO 3 - Cl - exchanger (PAT1) is depicted which exchanges luminal Cl - with cytoplasmic HCO 3 - . At 3 o’clock is depicted NBCe1 at the basolateral membrane which electrogenically transports 2–3 Na + and one HCO 3 - into the basolateral space. At 4 o’clock is Na + , K + /ATPase which pumps 3 Na + out of the cell into the blood stream and pumps in 2 K + inside the cell The topic of this manuscript deals with NBCe2, drawn at 8 o’clock. Under a normal sodium load it plays a minor role in Na + and HCO 3 - transport into the hRPTC. There are various plasma membrane receptors that regulate some of these transporters/ exchanger/pumps. The dopamine-1 receptor (D 1 R) (ten o’clock) when stimulated with dopamine (green box) inhibits (red lines) both NHE3 and Na + , K + /ATPase (without the red line, for simplicity) activities resulting in reduced Na + reabsorption and increased Na + excretion. The AT 1 R (5 o’clock) increases Na + , K + /ATPase activity (green arrow) resulting in increased Na + reabsorption. An increase in intracellular Na + increases Na + , K + /ATPase activity (5 o’clock) that is abetted by AT 1 R (green arrow) resulting in increased Na + transport from inside the cell to the basolateral space. The D 1 R and AT 1 R oppose each other. The D 1 R inhibits the AT 1 R, resulting in reduced Na + transport. We showed that NBCe2 is not affected by stimulation of the D 1 R or AT 1 R. Under basal conditions, NBCe1 is more active than NBCe2 (depicted as relatively larger directional transport arrows).

    Article Snippet: The dopamine-1 receptor (D1 R) (ten o’clock) when stimulated with dopamine (green box) inhibits (red lines) both NHE3 and Na+ ,K+ /ATPase (without the red line, for simplicity) activities resulting in reduced reabsorption and increased Na+ excretion.

    Techniques: Activity Assay

    Effect of HNF4A inhibitors on basal expression of NHE3, PAT1 and Na + ,K + /ATPase in hRPTCs. A) NHE3 protein expression Basal NHE3 protein expression is greater in HV than WT SLC4A5 hRPTCs (N = 3, *P

    Journal: PLoS ONE

    Article Title: Sodium bicarbonate cotransporter NBCe2 gene variants increase sodium and bicarbonate transport in human renal proximal tubule cells

    doi: 10.1371/journal.pone.0189464

    Figure Lengend Snippet: Effect of HNF4A inhibitors on basal expression of NHE3, PAT1 and Na + ,K + /ATPase in hRPTCs. A) NHE3 protein expression Basal NHE3 protein expression is greater in HV than WT SLC4A5 hRPTCs (N = 3, *P

    Article Snippet: The dopamine-1 receptor (D1 R) (ten o’clock) when stimulated with dopamine (green box) inhibits (red lines) both NHE3 and Na+ ,K+ /ATPase (without the red line, for simplicity) activities resulting in reduced reabsorption and increased Na+ excretion.

    Techniques: Expressing

    Effect of vanadate on renal α 1 -Na, K-ATPase protein abundance. Ten days of vanadate injection significantly suppressed α 1 -Na, K-ATPase protein abundance. Histogram bars show the densitometric analyses ratios of α 1 -Na, K-ATPase to β-actin intensity, and the representative immunoblot photographs are present. All values are expressed as the mean ± SD of 8 rats/group. *Significantly different from the sham group ( p

    Journal: Toxicological Research

    Article Title: Vanadate-Induced Renal cAMP and Malondialdehyde Accumulation Suppresses Alpha 1 Sodium Potassium Adenosine Triphosphatase Protein Levels

    doi: 10.5487/TR.2018.34.2.143

    Figure Lengend Snippet: Effect of vanadate on renal α 1 -Na, K-ATPase protein abundance. Ten days of vanadate injection significantly suppressed α 1 -Na, K-ATPase protein abundance. Histogram bars show the densitometric analyses ratios of α 1 -Na, K-ATPase to β-actin intensity, and the representative immunoblot photographs are present. All values are expressed as the mean ± SD of 8 rats/group. *Significantly different from the sham group ( p

    Article Snippet: Cyclic AMP phosphorylates Ser943 on the α subunit of Na, K-ATPase and reduces its activity in the rat TALH ( , ).

    Techniques: Injection

    Representative immunohistochemical staining images of renal α 1 -Na, K-ATPase protein localization in the cortex (A, D), outer medulla (B, E), and inner medulla (C, F) from sham (A–C), and vanadate (D–F) (n = 5/group). Vanadate reduced immunoreactivity both in the cortex (PCT and Pcap; arrows) and the medulla (PTs and MCD; arrow heads). Bar scale = 20 μm.

    Journal: Toxicological Research

    Article Title: Vanadate-Induced Renal cAMP and Malondialdehyde Accumulation Suppresses Alpha 1 Sodium Potassium Adenosine Triphosphatase Protein Levels

    doi: 10.5487/TR.2018.34.2.143

    Figure Lengend Snippet: Representative immunohistochemical staining images of renal α 1 -Na, K-ATPase protein localization in the cortex (A, D), outer medulla (B, E), and inner medulla (C, F) from sham (A–C), and vanadate (D–F) (n = 5/group). Vanadate reduced immunoreactivity both in the cortex (PCT and Pcap; arrows) and the medulla (PTs and MCD; arrow heads). Bar scale = 20 μm.

    Article Snippet: Cyclic AMP phosphorylates Ser943 on the α subunit of Na, K-ATPase and reduces its activity in the rat TALH ( , ).

    Techniques: Immunohistochemistry, Staining

    Intracellular localization of subunit a3 and markers for the Golgi apparatus and lysosomes in breast cancer cells MCF10a, MCF10CA1a, MB231, and SUM149 cells were grown on poly-d-lysine coated coverslips. To assess whether a3 localizes to the Golgi and lysosomes, cells were fixed, permeabilized, and immunostained using antibodies against subunit a3 of the V-ATPase and Giantin or LAMP2 as markers for the Golgi and lysosomes, respectively. Cells were then incubated with secondary antibodies as described under Materials and Methods. Images were taken with identical exposure times and antibody concentrations. A. Representative images of each of the cell lines stained for the Golgi apparatus marker Giantin ( left ), subunit a3 ( second from left ), the merged images ( second from right ), and the merged image magnified 3.5X ( labeled ‘Zoom’, far right ). B. Representative images of each of the cell lines stained for the lysosomal marker LAMP2 ( left ), subunit a3 ( second from left ), the merged images ( second from right ), and the inset of the merged image magnified 3.5X ( labeled ‘Zoom’, far right ). Colocalization is indicated by the presence of yellow pixels. The images in A and B depict representative staining from a minimum of two separate experiments, with an average of 50 cells imaged per experiment for each condition tested.

    Journal: Oncotarget

    Article Title: The a3 isoform of subunit a of the vacuolar ATPase localizes to the plasma membrane of invasive breast tumor cells and is overexpressed in human breast cancer

    doi: 10.18632/oncotarget.10063

    Figure Lengend Snippet: Intracellular localization of subunit a3 and markers for the Golgi apparatus and lysosomes in breast cancer cells MCF10a, MCF10CA1a, MB231, and SUM149 cells were grown on poly-d-lysine coated coverslips. To assess whether a3 localizes to the Golgi and lysosomes, cells were fixed, permeabilized, and immunostained using antibodies against subunit a3 of the V-ATPase and Giantin or LAMP2 as markers for the Golgi and lysosomes, respectively. Cells were then incubated with secondary antibodies as described under Materials and Methods. Images were taken with identical exposure times and antibody concentrations. A. Representative images of each of the cell lines stained for the Golgi apparatus marker Giantin ( left ), subunit a3 ( second from left ), the merged images ( second from right ), and the merged image magnified 3.5X ( labeled ‘Zoom’, far right ). B. Representative images of each of the cell lines stained for the lysosomal marker LAMP2 ( left ), subunit a3 ( second from left ), the merged images ( second from right ), and the inset of the merged image magnified 3.5X ( labeled ‘Zoom’, far right ). Colocalization is indicated by the presence of yellow pixels. The images in A and B depict representative staining from a minimum of two separate experiments, with an average of 50 cells imaged per experiment for each condition tested.

    Article Snippet: Briefly, an siRNA pool specific for the subunit a3 isoform of the V-ATPase was purchased from Dharmacon.

    Techniques: Incubation, Staining, Marker, Labeling

    Subunit a3 colocalizes with a leading edge marker in migrating breast cancer cells MCF10CA1a, MB231, and SUM149 breast cancer cells were grown to confluence on poly-d-lysine coated coverslips. A wound was made in the cell monolayer and cells were allowed to migrate for 4 h in order to induce the formation of a leading edge. Cells were then fixed, permeabilized, and immunostained using antibodies against subunit a3 of the V-ATPase and the leading edge marker cortactin followed by incubation with secondary antibodies as described under Materials and Methods. Images were taken with identical exposure times and antibody concentrations. Shown are representative images of MCF10CA1a, MB231, and SUM149 cells stained for cortactin ( left ), a3 ( second from left ), and DAPI ( second from right ). The merged images are present to the far right. White arrows indicate subunit a3 and cortactin colocalization at the leading edge. The images depict representative staining from a minimum of two separate experiments, with 50-80 cells imaged per experiment for each condition tested.

    Journal: Oncotarget

    Article Title: The a3 isoform of subunit a of the vacuolar ATPase localizes to the plasma membrane of invasive breast tumor cells and is overexpressed in human breast cancer

    doi: 10.18632/oncotarget.10063

    Figure Lengend Snippet: Subunit a3 colocalizes with a leading edge marker in migrating breast cancer cells MCF10CA1a, MB231, and SUM149 breast cancer cells were grown to confluence on poly-d-lysine coated coverslips. A wound was made in the cell monolayer and cells were allowed to migrate for 4 h in order to induce the formation of a leading edge. Cells were then fixed, permeabilized, and immunostained using antibodies against subunit a3 of the V-ATPase and the leading edge marker cortactin followed by incubation with secondary antibodies as described under Materials and Methods. Images were taken with identical exposure times and antibody concentrations. Shown are representative images of MCF10CA1a, MB231, and SUM149 cells stained for cortactin ( left ), a3 ( second from left ), and DAPI ( second from right ). The merged images are present to the far right. White arrows indicate subunit a3 and cortactin colocalization at the leading edge. The images depict representative staining from a minimum of two separate experiments, with 50-80 cells imaged per experiment for each condition tested.

    Article Snippet: Briefly, an siRNA pool specific for the subunit a3 isoform of the V-ATPase was purchased from Dharmacon.

    Techniques: Marker, Incubation, Staining

    Intracellular localization of subunit a3 and markers for early and late endosomes in breast cancer cells MCF10a, MCF10CA1a, MB231, and SUM149 cells were grown on poly-d-lysine coated coverslips. To assess whether a3 localizes to endosomal compartments, cells were fixed, permeabilized, and immunostained using antibodies against subunit a3 of the V-ATPase and EEA1 or Rab7 as markers for early endosomes and late endosomes, respectively. Cells were then incubated with secondary antibodies as described under Materials and Methods. Images were taken with identical exposure times and antibody concentrations. A. Representative images of each of the cell lines stained for the early endosome marker EEA1 ( left ), subunit a3 ( second from left ), the merged images ( second from right ), and the inset of the merged image magnified 3.5X ( labeled ‘Zoom’, far right ). B. Representative images of each of the cell lines stained for the late endosome marker Rab7 ( left ), subunit a3 ( second from left ), the merged images ( second from right ), and the merged image magnified 3.5X ( labeled ‘Zoom’, far right ). Colocalization is indicated by the presence of yellow pixels. The images in A and B depict representative staining from a minimum of two separate experiments, with an average of 50 cells imaged per experiment for each condition tested.

    Journal: Oncotarget

    Article Title: The a3 isoform of subunit a of the vacuolar ATPase localizes to the plasma membrane of invasive breast tumor cells and is overexpressed in human breast cancer

    doi: 10.18632/oncotarget.10063

    Figure Lengend Snippet: Intracellular localization of subunit a3 and markers for early and late endosomes in breast cancer cells MCF10a, MCF10CA1a, MB231, and SUM149 cells were grown on poly-d-lysine coated coverslips. To assess whether a3 localizes to endosomal compartments, cells were fixed, permeabilized, and immunostained using antibodies against subunit a3 of the V-ATPase and EEA1 or Rab7 as markers for early endosomes and late endosomes, respectively. Cells were then incubated with secondary antibodies as described under Materials and Methods. Images were taken with identical exposure times and antibody concentrations. A. Representative images of each of the cell lines stained for the early endosome marker EEA1 ( left ), subunit a3 ( second from left ), the merged images ( second from right ), and the inset of the merged image magnified 3.5X ( labeled ‘Zoom’, far right ). B. Representative images of each of the cell lines stained for the late endosome marker Rab7 ( left ), subunit a3 ( second from left ), the merged images ( second from right ), and the merged image magnified 3.5X ( labeled ‘Zoom’, far right ). Colocalization is indicated by the presence of yellow pixels. The images in A and B depict representative staining from a minimum of two separate experiments, with an average of 50 cells imaged per experiment for each condition tested.

    Article Snippet: Briefly, an siRNA pool specific for the subunit a3 isoform of the V-ATPase was purchased from Dharmacon.

    Techniques: Incubation, Staining, Marker, Labeling

    Subunit a3 localizes to the plasma membrane of human breast cancer cells MCF10a, MCF10CA1a, MB231, and SUM149 cells were grown to confluence on poly-d-lysine coated coverslips. To assess the effects of cell migration on a3 localization, a wound was made in the cell monolayer and cells were allowed to migrate for 4 h. Cells were then fixed, permeabilized, and immunostained using antibodies against subunit a3 of the V-ATPase and Alexa Fluor® 568 phalloidin to stain actin followed by incubation with secondary antibodies as described under Materials and Methods. Images were taken with identical exposure times and antibody concentrations. A. Shown are representative images of confluent MCF10a, MCF10CA1a, MB231, and SUM149 cells stained for phalloidin ( left ), subunit a3 (second from left ), DAPI ( second from right ), and the merged images ( right ). B. Shown are representative images of MCF10a, MCF10CA1a, MB231, and SUM149 cells (with monolayer wounded) stained for phalloidin ( left ), subunit a3 (second from left ), DAPI ( second from right ), and the merged images ( right ). White arrows indicate plasma membrane subunit a3. C. Quantification of plasma membrane staining for subunit a3 in confluent or migrating cells. To quantitate plasma membrane staining, 50-80 cells from three separate experiments were counted and the number of cells showing plasma membrane a3 localization was determined. The graphed data represents the percentage of cells showing a3 localization to the plasma membrane. All error bars indicate S.E. *, p

    Journal: Oncotarget

    Article Title: The a3 isoform of subunit a of the vacuolar ATPase localizes to the plasma membrane of invasive breast tumor cells and is overexpressed in human breast cancer

    doi: 10.18632/oncotarget.10063

    Figure Lengend Snippet: Subunit a3 localizes to the plasma membrane of human breast cancer cells MCF10a, MCF10CA1a, MB231, and SUM149 cells were grown to confluence on poly-d-lysine coated coverslips. To assess the effects of cell migration on a3 localization, a wound was made in the cell monolayer and cells were allowed to migrate for 4 h. Cells were then fixed, permeabilized, and immunostained using antibodies against subunit a3 of the V-ATPase and Alexa Fluor® 568 phalloidin to stain actin followed by incubation with secondary antibodies as described under Materials and Methods. Images were taken with identical exposure times and antibody concentrations. A. Shown are representative images of confluent MCF10a, MCF10CA1a, MB231, and SUM149 cells stained for phalloidin ( left ), subunit a3 (second from left ), DAPI ( second from right ), and the merged images ( right ). B. Shown are representative images of MCF10a, MCF10CA1a, MB231, and SUM149 cells (with monolayer wounded) stained for phalloidin ( left ), subunit a3 (second from left ), DAPI ( second from right ), and the merged images ( right ). White arrows indicate plasma membrane subunit a3. C. Quantification of plasma membrane staining for subunit a3 in confluent or migrating cells. To quantitate plasma membrane staining, 50-80 cells from three separate experiments were counted and the number of cells showing plasma membrane a3 localization was determined. The graphed data represents the percentage of cells showing a3 localization to the plasma membrane. All error bars indicate S.E. *, p

    Article Snippet: Briefly, an siRNA pool specific for the subunit a3 isoform of the V-ATPase was purchased from Dharmacon.

    Techniques: Migration, Staining, Incubation

    ( A ) Triple immunofluorescence labeling of a kidney section from AQP2-cre + tdTomato + (PC reporter) mice showing: AQP2 (red cells, upper left);. V-ATPase E1 (green cells, ICs, upper right); tdTomato (blue cells, lower left) staining in inner medulla. On the merged image (lower right) most AQP2 cells were also positive for tdTomato (purple cells, white arrows) while some were negative for tdTomato (red cells, white arrowheads). The dashed box contains a tubule imaged from another kidney section that contained an isolated cell that was positive for both AQP2 and V-ATPAse E1 (Yellow cell, asterisk). Magnification 40X, confocal microscopy. ( B ) Triple immunofluorescence labeling of a kidney section from V-ATPase B1-cre + tdTomato + (IC reporter) mice showing: V-ATPase B1 (red cells, upper left). tdTomato (green cells, upper right) and nuclei (blue, lower left) staining in inner medulla. On the merged image (lower right) most V-ATPase B1 positive cells were also tdTomato positive (yellow cells, white arrows) while a few cells were tdTomato positive but were V-ATPase B1 negative (green cells, white arrowheads). Magnification 20X, immunofluorescent microscopy.

    Journal: Scientific Reports

    Article Title: Whole Transcriptome Analysis of Renal Intercalated Cells Predicts Lipopolysaccharide Mediated Inhibition of Retinoid X Receptor alpha Function

    doi: 10.1038/s41598-018-36921-z

    Figure Lengend Snippet: ( A ) Triple immunofluorescence labeling of a kidney section from AQP2-cre + tdTomato + (PC reporter) mice showing: AQP2 (red cells, upper left);. V-ATPase E1 (green cells, ICs, upper right); tdTomato (blue cells, lower left) staining in inner medulla. On the merged image (lower right) most AQP2 cells were also positive for tdTomato (purple cells, white arrows) while some were negative for tdTomato (red cells, white arrowheads). The dashed box contains a tubule imaged from another kidney section that contained an isolated cell that was positive for both AQP2 and V-ATPAse E1 (Yellow cell, asterisk). Magnification 40X, confocal microscopy. ( B ) Triple immunofluorescence labeling of a kidney section from V-ATPase B1-cre + tdTomato + (IC reporter) mice showing: V-ATPase B1 (red cells, upper left). tdTomato (green cells, upper right) and nuclei (blue, lower left) staining in inner medulla. On the merged image (lower right) most V-ATPase B1 positive cells were also tdTomato positive (yellow cells, white arrows) while a few cells were tdTomato positive but were V-ATPase B1 negative (green cells, white arrowheads). Magnification 20X, immunofluorescent microscopy.

    Article Snippet: V-ATPase-cre mice : In this mice, to visualize, V-ATPase, goat anti-mouse V-ATPase B1/B2 antibody (E20) (sc21209, 1:50 dilution, Santa Cruz, CA), to visualize tdTomato, anti-RFP-FITC conjugated antibody (ab34764, 1:200 dilution, abcam, MA) were incubated overnight at 4 °C.

    Techniques: Immunofluorescence, Labeling, Mouse Assay, Staining, Isolation, Confocal Microscopy, Microscopy

    STED microscopy dissecting the localization of Na + ,K + -ATPase in cultured striatal neurons . The 5 × 3 mosaic shows a comparision of the confocal (left) and STED images (middle) of the Alexa-594 immunolabelled α3 NKA in dendritic spines. Postprocessing of the raw STED data by a Richardson-Lucy deconvolution further enhances the details as shown in the right row of images. Scale bar: 500 nm

    Journal: BMC Neuroscience

    Article Title: Spatial distribution of Na+-K+-ATPase in dendritic spines dissected by nanoscale superresolution STED microscopy

    doi: 10.1186/1471-2202-12-16

    Figure Lengend Snippet: STED microscopy dissecting the localization of Na + ,K + -ATPase in cultured striatal neurons . The 5 × 3 mosaic shows a comparision of the confocal (left) and STED images (middle) of the Alexa-594 immunolabelled α3 NKA in dendritic spines. Postprocessing of the raw STED data by a Richardson-Lucy deconvolution further enhances the details as shown in the right row of images. Scale bar: 500 nm

    Article Snippet: The primary antibody was mouse monoclonal anti-α3 Na + ,K+ -ATPase (Affinity bioreagents).

    Techniques: Microscopy, Cell Culture

    The immunoblots and relative intensities of Prdx6 in membrane-fraction proteins of livers of the fresh water (FW) and seawater (SW) milkfish acclimated to 28°C (white bar) and 18°C (stripe bar) for 1 week, respectively . In the representative immunoblot, single immunoreactive bands at 100 and 25 kDa represent Na + , K + -ATPase (NKA), and Prdx6, respectively. The NKA was used as the loading control of the membrane fraction. Different letters (a and b) indicate significant differences between the 28° and 18°C group, and (x and y) indicate significant differences between the FW and SW group. Values are means ± SEM, n = 6. The Student's t -test pairwise comparison was used following two-way ANOVA, P

    Journal: Frontiers in Physiology

    Article Title: The Antioxidant Peroxiredoxin 6 (Prdx6) Exhibits Different Profiles in the Livers of Seawater- and Fresh Water-Acclimated Milkfish, Chanos chanos, upon Hypothermal Challenge

    doi: 10.3389/fphys.2016.00580

    Figure Lengend Snippet: The immunoblots and relative intensities of Prdx6 in membrane-fraction proteins of livers of the fresh water (FW) and seawater (SW) milkfish acclimated to 28°C (white bar) and 18°C (stripe bar) for 1 week, respectively . In the representative immunoblot, single immunoreactive bands at 100 and 25 kDa represent Na + , K + -ATPase (NKA), and Prdx6, respectively. The NKA was used as the loading control of the membrane fraction. Different letters (a and b) indicate significant differences between the 28° and 18°C group, and (x and y) indicate significant differences between the FW and SW group. Values are means ± SEM, n = 6. The Student's t -test pairwise comparison was used following two-way ANOVA, P

    Article Snippet: Then, the immunoblotting was performed as described above except that Na+ , K+ -ATPase (NKA; α5, Developmental Studies Hybridoma Bank, Iowa City, IA, USA) was used as the loading control.

    Techniques: Western Blot