atp Thermo Fisher Search Results


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  • 97
    Thermo Fisher atp
    Atp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 727 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher gene exp mnat1 hs01041574 m1
    CDK7, Cyclin H, and <t>MAT1</t> expression is associated with better patient outcome in breast cancer
    Gene Exp Mnat1 Hs01041574 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher unlabeled atp
    CDK7, Cyclin H, and <t>MAT1</t> expression is associated with better patient outcome in breast cancer
    Unlabeled Atp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    Thermo Fisher atp probe
    CDK7, Cyclin H, and <t>MAT1</t> expression is associated with better patient outcome in breast cancer
    Atp Probe, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 82/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 82 stars, based on 7 article reviews
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    78
    Thermo Fisher bodipyfl atp
    CDK7, Cyclin H, and <t>MAT1</t> expression is associated with better patient outcome in breast cancer
    Bodipyfl Atp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 78 stars, based on 4 article reviews
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    83
    Thermo Fisher etheno atp
    CDK7, Cyclin H, and <t>MAT1</t> expression is associated with better patient outcome in breast cancer
    Etheno Atp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    Thermo Fisher ε atp
    CDK7, Cyclin H, and <t>MAT1</t> expression is associated with better patient outcome in breast cancer
    ε Atp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 82/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Thermo Fisher e atp
    Proposed model of coordinated nucleotide metabolism and nucleoside signaling in posthypoxic endothelial cells: in areas of ongoing inflammation, diminished oxygen supply coordinates the induction of CD39, CD73, and AdoRA 2B . At such sites, activated PMN provide a readily available extracellular source of <t>ATP</t> that through two enzymatic steps results in the liberation of extracellular adenosine. Adenosine generated in this fashion is available for activation of surface endothelial adenosine receptors, particularly the AdoRA 2B . Postreceptor increases in intracellular cyclic <t>AMP</t> results in enhanced barrier function. As such, this protective mechanism may provide an innate mechanism to preserve vascular integrity and prevent fulminant intravascular fluid loss.
    E Atp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher atp determination kit
    Proposed model of coordinated nucleotide metabolism and nucleoside signaling in posthypoxic endothelial cells: in areas of ongoing inflammation, diminished oxygen supply coordinates the induction of CD39, CD73, and AdoRA 2B . At such sites, activated PMN provide a readily available extracellular source of <t>ATP</t> that through two enzymatic steps results in the liberation of extracellular adenosine. Adenosine generated in this fashion is available for activation of surface endothelial adenosine receptors, particularly the AdoRA 2B . Postreceptor increases in intracellular cyclic <t>AMP</t> results in enhanced barrier function. As such, this protective mechanism may provide an innate mechanism to preserve vascular integrity and prevent fulminant intravascular fluid loss.
    Atp Determination Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2257 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher atp detection kit
    Proposed model of coordinated nucleotide metabolism and nucleoside signaling in posthypoxic endothelial cells: in areas of ongoing inflammation, diminished oxygen supply coordinates the induction of CD39, CD73, and AdoRA 2B . At such sites, activated PMN provide a readily available extracellular source of <t>ATP</t> that through two enzymatic steps results in the liberation of extracellular adenosine. Adenosine generated in this fashion is available for activation of surface endothelial adenosine receptors, particularly the AdoRA 2B . Postreceptor increases in intracellular cyclic <t>AMP</t> results in enhanced barrier function. As such, this protective mechanism may provide an innate mechanism to preserve vascular integrity and prevent fulminant intravascular fluid loss.
    Atp Detection Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher atp assay kit
    <t>HNK</t> affect energy homeostasis of cancer cells by effecting cellular <t>ATP.</t> (a) Linear standard curves were generated using pure standards of ATP and ADP with R 2 value greater than 0.99 for both ATP and ADP for HPLC. (b) ATP modulation by HNK in B16/F-10 and SKMEL-28 as measured by in a Luciferase based assay. No changes in ATP levels were seen at 24 h, but a statistically significant decrease in ATP levels was seen only in SKMEL-28 cells at 48 h. (c) HPLC was used to evaluate ATP and ADP levels in both cells lines at 24 and 48 h. No changes were seen at 24 h, but ATP levels were statistically lower in both cell lines at 48 h. No changes in ADP levels were observed.
    Atp Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    74
    Thermo Fisher desthiobiotin atp
    Kinase profiling of U2OS cell line in the presence of circadian period–lengthening compounds. (A) Chemical structure and mechanism of the <t>desthiobiotin-ATP</t> probe for kinase active-site labelling. Desthiobiotin-ATP probes are nucleotide derivatives, which covalently modify the active site of enzymes (kinases) with conserved lysine residues in the nucleotide-binding site. The structure of desthiobiotin-ATP consists of a modified biotin attached to the nucleotide by a labile acyl-phosphate bond. (B) Enrichment efficiency of desthiobiotin-ATP labelled (active-site labelled) peptides in quantitative mass spectrometric analysis (n = 3). (C) Kinome profile of U2OS cell line after treatment with circadian period–lengthening compounds (25 μM). Heat map representation of relative abundance (against DMSO control) of 174 kinases in longdaysin-, purvalanol A–, roscovitine-, and SP600125-treated cell lysates (mean, n = 3). Relative expression and inhibition of kinases were measured using TMT-based quantitation by MS.
    Desthiobiotin Atp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 74/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    76
    Thermo Fisher bioluminescence atp determination assay
    Kinase profiling of U2OS cell line in the presence of circadian period–lengthening compounds. (A) Chemical structure and mechanism of the <t>desthiobiotin-ATP</t> probe for kinase active-site labelling. Desthiobiotin-ATP probes are nucleotide derivatives, which covalently modify the active site of enzymes (kinases) with conserved lysine residues in the nucleotide-binding site. The structure of desthiobiotin-ATP consists of a modified biotin attached to the nucleotide by a labile acyl-phosphate bond. (B) Enrichment efficiency of desthiobiotin-ATP labelled (active-site labelled) peptides in quantitative mass spectrometric analysis (n = 3). (C) Kinome profile of U2OS cell line after treatment with circadian period–lengthening compounds (25 μM). Heat map representation of relative abundance (against DMSO control) of 174 kinases in longdaysin-, purvalanol A–, roscovitine-, and SP600125-treated cell lysates (mean, n = 3). Relative expression and inhibition of kinases were measured using TMT-based quantitation by MS.
    Bioluminescence Atp Determination Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 76/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher biotinylated 14 atp
    Kinase profiling of U2OS cell line in the presence of circadian period–lengthening compounds. (A) Chemical structure and mechanism of the <t>desthiobiotin-ATP</t> probe for kinase active-site labelling. Desthiobiotin-ATP probes are nucleotide derivatives, which covalently modify the active site of enzymes (kinases) with conserved lysine residues in the nucleotide-binding site. The structure of desthiobiotin-ATP consists of a modified biotin attached to the nucleotide by a labile acyl-phosphate bond. (B) Enrichment efficiency of desthiobiotin-ATP labelled (active-site labelled) peptides in quantitative mass spectrometric analysis (n = 3). (C) Kinome profile of U2OS cell line after treatment with circadian period–lengthening compounds (25 μM). Heat map representation of relative abundance (against DMSO control) of 174 kinases in longdaysin-, purvalanol A–, roscovitine-, and SP600125-treated cell lysates (mean, n = 3). Relative expression and inhibition of kinases were measured using TMT-based quantitation by MS.
    Biotinylated 14 Atp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher biotin 14 atp
    Kinase profiling of U2OS cell line in the presence of circadian period–lengthening compounds. (A) Chemical structure and mechanism of the <t>desthiobiotin-ATP</t> probe for kinase active-site labelling. Desthiobiotin-ATP probes are nucleotide derivatives, which covalently modify the active site of enzymes (kinases) with conserved lysine residues in the nucleotide-binding site. The structure of desthiobiotin-ATP consists of a modified biotin attached to the nucleotide by a labile acyl-phosphate bond. (B) Enrichment efficiency of desthiobiotin-ATP labelled (active-site labelled) peptides in quantitative mass spectrometric analysis (n = 3). (C) Kinome profile of U2OS cell line after treatment with circadian period–lengthening compounds (25 μM). Heat map representation of relative abundance (against DMSO control) of 174 kinases in longdaysin-, purvalanol A–, roscovitine-, and SP600125-treated cell lysates (mean, n = 3). Relative expression and inhibition of kinases were measured using TMT-based quantitation by MS.
    Biotin 14 Atp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    Thermo Fisher biotinylated dideoxy atp
    Kinase profiling of U2OS cell line in the presence of circadian period–lengthening compounds. (A) Chemical structure and mechanism of the <t>desthiobiotin-ATP</t> probe for kinase active-site labelling. Desthiobiotin-ATP probes are nucleotide derivatives, which covalently modify the active site of enzymes (kinases) with conserved lysine residues in the nucleotide-binding site. The structure of desthiobiotin-ATP consists of a modified biotin attached to the nucleotide by a labile acyl-phosphate bond. (B) Enrichment efficiency of desthiobiotin-ATP labelled (active-site labelled) peptides in quantitative mass spectrometric analysis (n = 3). (C) Kinome profile of U2OS cell line after treatment with circadian period–lengthening compounds (25 μM). Heat map representation of relative abundance (against DMSO control) of 174 kinases in longdaysin-, purvalanol A–, roscovitine-, and SP600125-treated cell lysates (mean, n = 3). Relative expression and inhibition of kinases were measured using TMT-based quantitation by MS.
    Biotinylated Dideoxy Atp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    83
    Thermo Fisher n6 6 aminohexyl atp
    Kinase profiling of U2OS cell line in the presence of circadian period–lengthening compounds. (A) Chemical structure and mechanism of the <t>desthiobiotin-ATP</t> probe for kinase active-site labelling. Desthiobiotin-ATP probes are nucleotide derivatives, which covalently modify the active site of enzymes (kinases) with conserved lysine residues in the nucleotide-binding site. The structure of desthiobiotin-ATP consists of a modified biotin attached to the nucleotide by a labile acyl-phosphate bond. (B) Enrichment efficiency of desthiobiotin-ATP labelled (active-site labelled) peptides in quantitative mass spectrometric analysis (n = 3). (C) Kinome profile of U2OS cell line after treatment with circadian period–lengthening compounds (25 μM). Heat map representation of relative abundance (against DMSO control) of 174 kinases in longdaysin-, purvalanol A–, roscovitine-, and SP600125-treated cell lysates (mean, n = 3). Relative expression and inhibition of kinases were measured using TMT-based quantitation by MS.
    N6 6 Aminohexyl Atp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    76
    Thermo Fisher 1 n6 etheno atp
    Kinase profiling of U2OS cell line in the presence of circadian period–lengthening compounds. (A) Chemical structure and mechanism of the <t>desthiobiotin-ATP</t> probe for kinase active-site labelling. Desthiobiotin-ATP probes are nucleotide derivatives, which covalently modify the active site of enzymes (kinases) with conserved lysine residues in the nucleotide-binding site. The structure of desthiobiotin-ATP consists of a modified biotin attached to the nucleotide by a labile acyl-phosphate bond. (B) Enrichment efficiency of desthiobiotin-ATP labelled (active-site labelled) peptides in quantitative mass spectrometric analysis (n = 3). (C) Kinome profile of U2OS cell line after treatment with circadian period–lengthening compounds (25 μM). Heat map representation of relative abundance (against DMSO control) of 174 kinases in longdaysin-, purvalanol A–, roscovitine-, and SP600125-treated cell lysates (mean, n = 3). Relative expression and inhibition of kinases were measured using TMT-based quantitation by MS.
    1 N6 Etheno Atp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 76/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Thermo Fisher colorimetric atp measurement kit
    Kinase profiling of U2OS cell line in the presence of circadian period–lengthening compounds. (A) Chemical structure and mechanism of the <t>desthiobiotin-ATP</t> probe for kinase active-site labelling. Desthiobiotin-ATP probes are nucleotide derivatives, which covalently modify the active site of enzymes (kinases) with conserved lysine residues in the nucleotide-binding site. The structure of desthiobiotin-ATP consists of a modified biotin attached to the nucleotide by a labile acyl-phosphate bond. (B) Enrichment efficiency of desthiobiotin-ATP labelled (active-site labelled) peptides in quantitative mass spectrometric analysis (n = 3). (C) Kinome profile of U2OS cell line after treatment with circadian period–lengthening compounds (25 μM). Heat map representation of relative abundance (against DMSO control) of 174 kinases in longdaysin-, purvalanol A–, roscovitine-, and SP600125-treated cell lysates (mean, n = 3). Relative expression and inhibition of kinases were measured using TMT-based quantitation by MS.
    Colorimetric Atp Measurement Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Thermo Fisher γ 32 p atp
    Kinase profiling of U2OS cell line in the presence of circadian period–lengthening compounds. (A) Chemical structure and mechanism of the <t>desthiobiotin-ATP</t> probe for kinase active-site labelling. Desthiobiotin-ATP probes are nucleotide derivatives, which covalently modify the active site of enzymes (kinases) with conserved lysine residues in the nucleotide-binding site. The structure of desthiobiotin-ATP consists of a modified biotin attached to the nucleotide by a labile acyl-phosphate bond. (B) Enrichment efficiency of desthiobiotin-ATP labelled (active-site labelled) peptides in quantitative mass spectrometric analysis (n = 3). (C) Kinome profile of U2OS cell line after treatment with circadian period–lengthening compounds (25 μM). Heat map representation of relative abundance (against DMSO control) of 174 kinases in longdaysin-, purvalanol A–, roscovitine-, and SP600125-treated cell lysates (mean, n = 3). Relative expression and inhibition of kinases were measured using TMT-based quantitation by MS.
    γ 32 P Atp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 2272 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher atp desthiobiotin kit
    Semapimod abrogates modification of gp96 with <t>ATP-desthiobiotin.</t> (A) Silver-stained gel of IEC-6 proteins modified by ATP-desthiobiotin and collected on streptavidin-agarose. Cells were pre-treated with 10 μM Semapimod for 15 min and then treated
    Atp Desthiobiotin Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher atp reaction mix
    Semapimod abrogates modification of gp96 with <t>ATP-desthiobiotin.</t> (A) Silver-stained gel of IEC-6 proteins modified by ATP-desthiobiotin and collected on streptavidin-agarose. Cells were pre-treated with 10 μM Semapimod for 15 min and then treated
    Atp Reaction Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    81
    Thermo Fisher triton x 100 thermo fisher kinase
    Semapimod abrogates modification of gp96 with <t>ATP-desthiobiotin.</t> (A) Silver-stained gel of IEC-6 proteins modified by ATP-desthiobiotin and collected on streptavidin-agarose. Cells were pre-treated with 10 μM Semapimod for 15 min and then treated
    Triton X 100 Thermo Fisher Kinase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Thermo Fisher non hydrolysable atp analogue amppcp
    Semapimod abrogates modification of gp96 with <t>ATP-desthiobiotin.</t> (A) Silver-stained gel of IEC-6 proteins modified by ATP-desthiobiotin and collected on streptavidin-agarose. Cells were pre-treated with 10 μM Semapimod for 15 min and then treated
    Non Hydrolysable Atp Analogue Amppcp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher nonradioactive atp
    Semapimod abrogates modification of gp96 with <t>ATP-desthiobiotin.</t> (A) Silver-stained gel of IEC-6 proteins modified by ATP-desthiobiotin and collected on streptavidin-agarose. Cells were pre-treated with 10 μM Semapimod for 15 min and then treated
    Nonradioactive Atp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher luminescent atp determination kit purchase
    Semapimod abrogates modification of gp96 with <t>ATP-desthiobiotin.</t> (A) Silver-stained gel of IEC-6 proteins modified by ATP-desthiobiotin and collected on streptavidin-agarose. Cells were pre-treated with 10 μM Semapimod for 15 min and then treated
    Luminescent Atp Determination Kit Purchase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher luciferase based atp kit
    Semapimod abrogates modification of gp96 with <t>ATP-desthiobiotin.</t> (A) Silver-stained gel of IEC-6 proteins modified by ATP-desthiobiotin and collected on streptavidin-agarose. Cells were pre-treated with 10 μM Semapimod for 15 min and then treated
    Luciferase Based Atp Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 76/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    Thermo Fisher atp bioluminescence determination assay kit
    MN induced mitochondrial dysfunction. <t>HCEnC,</t> HCECi, and FECDi cells were exposed to MN in a dose-dependent manner for 1 h with or without NAC pretreatment and collected for measurement of mitochondrial ROS production by flow cytometry, <t>ATP</t> levels,
    Atp Bioluminescence Determination Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    76
    Thermo Fisher cdk7 cyclin h mnat1 recombinant human protein
    MN induced mitochondrial dysfunction. <t>HCEnC,</t> HCECi, and FECDi cells were exposed to MN in a dose-dependent manner for 1 h with or without NAC pretreatment and collected for measurement of mitochondrial ROS production by flow cytometry, <t>ATP</t> levels,
    Cdk7 Cyclin H Mnat1 Recombinant Human Protein, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 76/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    CDK7, Cyclin H, and MAT1 expression is associated with better patient outcome in breast cancer

    Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

    Article Title: Expression of CDK7, Cyclin H, and MAT1 Is Elevated in Breast Cancer and Is Prognostic in Estrogen Receptor–Positive Breast Cancer

    doi: 10.1158/1078-0432.CCR-15-1104

    Figure Lengend Snippet: CDK7, Cyclin H, and MAT1 expression is associated with better patient outcome in breast cancer

    Article Snippet: Quantitative gene expression analysis was carried out using real-time PCR and Taqman gene expression assays for CDK7 (Hs00361486_m1), Cyclin H (Hs00236923_m1), MAT1 (Hs01041574_m1), and GAPDH (Hs99999905_m1; Life Technologies).

    Techniques: Expressing

    CDK7, Cyclin H, and MAT1 expression is associated with better patient outcome in breast cancer

    Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

    Article Title: Expression of CDK7, Cyclin H, and MAT1 Is Elevated in Breast Cancer and Is Prognostic in Estrogen Receptor–Positive Breast Cancer

    doi: 10.1158/1078-0432.CCR-15-1104

    Figure Lengend Snippet: CDK7, Cyclin H, and MAT1 expression is associated with better patient outcome in breast cancer

    Article Snippet: Quantitative gene expression analysis was carried out using real-time PCR and Taqman gene expression assays for CDK7 (Hs00361486_m1), Cyclin H (Hs00236923_m1), MAT1 (Hs01041574_m1), and GAPDH (Hs99999905_m1; Life Technologies).

    Techniques: Expressing

    Immunohistochemical analysis of CDK7, Cyclin H, and MAT1 expression in breast cancer. CDK7, Cyclin H, and MAT1 antibodies were used to immunostain breast cancer TMAs. The sections were scored as CDK7 low (H-score: 0–160), CDK7 high (H-score: 161–300),

    Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

    Article Title: Expression of CDK7, Cyclin H, and MAT1 Is Elevated in Breast Cancer and Is Prognostic in Estrogen Receptor–Positive Breast Cancer

    doi: 10.1158/1078-0432.CCR-15-1104

    Figure Lengend Snippet: Immunohistochemical analysis of CDK7, Cyclin H, and MAT1 expression in breast cancer. CDK7, Cyclin H, and MAT1 antibodies were used to immunostain breast cancer TMAs. The sections were scored as CDK7 low (H-score: 0–160), CDK7 high (H-score: 161–300),

    Article Snippet: Quantitative gene expression analysis was carried out using real-time PCR and Taqman gene expression assays for CDK7 (Hs00361486_m1), Cyclin H (Hs00236923_m1), MAT1 (Hs01041574_m1), and GAPDH (Hs99999905_m1; Life Technologies).

    Techniques: Immunohistochemistry, Expressing

    CDK7, Cyclin H, and MAT1 mRNA levels are elevated in breast cancer and show evidence of coordinate regulation. A–C , CDK7, Cyclin H, and MAT1 mRNA levels, determined by real-time RT-PCR analysis, were normalized to the expression of GAPDH for RNA

    Journal: Clinical cancer research : an official journal of the American Association for Cancer Research

    Article Title: Expression of CDK7, Cyclin H, and MAT1 Is Elevated in Breast Cancer and Is Prognostic in Estrogen Receptor–Positive Breast Cancer

    doi: 10.1158/1078-0432.CCR-15-1104

    Figure Lengend Snippet: CDK7, Cyclin H, and MAT1 mRNA levels are elevated in breast cancer and show evidence of coordinate regulation. A–C , CDK7, Cyclin H, and MAT1 mRNA levels, determined by real-time RT-PCR analysis, were normalized to the expression of GAPDH for RNA

    Article Snippet: Quantitative gene expression analysis was carried out using real-time PCR and Taqman gene expression assays for CDK7 (Hs00361486_m1), Cyclin H (Hs00236923_m1), MAT1 (Hs01041574_m1), and GAPDH (Hs99999905_m1; Life Technologies).

    Techniques: Quantitative RT-PCR, Expressing

    Proposed model of coordinated nucleotide metabolism and nucleoside signaling in posthypoxic endothelial cells: in areas of ongoing inflammation, diminished oxygen supply coordinates the induction of CD39, CD73, and AdoRA 2B . At such sites, activated PMN provide a readily available extracellular source of ATP that through two enzymatic steps results in the liberation of extracellular adenosine. Adenosine generated in this fashion is available for activation of surface endothelial adenosine receptors, particularly the AdoRA 2B . Postreceptor increases in intracellular cyclic AMP results in enhanced barrier function. As such, this protective mechanism may provide an innate mechanism to preserve vascular integrity and prevent fulminant intravascular fluid loss.

    Journal: The Journal of Experimental Medicine

    Article Title: Coordinated Adenine Nucleotide Phosphohydrolysis and Nucleoside Signaling in Posthypoxic Endothelium

    doi: 10.1084/jem.20030891

    Figure Lengend Snippet: Proposed model of coordinated nucleotide metabolism and nucleoside signaling in posthypoxic endothelial cells: in areas of ongoing inflammation, diminished oxygen supply coordinates the induction of CD39, CD73, and AdoRA 2B . At such sites, activated PMN provide a readily available extracellular source of ATP that through two enzymatic steps results in the liberation of extracellular adenosine. Adenosine generated in this fashion is available for activation of surface endothelial adenosine receptors, particularly the AdoRA 2B . Postreceptor increases in intracellular cyclic AMP results in enhanced barrier function. As such, this protective mechanism may provide an innate mechanism to preserve vascular integrity and prevent fulminant intravascular fluid loss.

    Article Snippet: Ratio of E-ATP (Molecular Probes Inc.) and E-AMP (Sigma-Aldrich) was measured with a 0–50% methanol/H2 O gradient mobile phase (2 ml/min over 10 min).

    Techniques: Generated, Activation Assay

    Induction of functional CD39 is by hypoxia. (A) Confluent HMEC-1 monolayers were exposed to normoxia (pO 2 147 torr, 18 h) or hypoxia (pO 2 20 torr, 18 h). Total RNA was isolated, and CD39 mRNA levels were determined by RT-PCR using semiquantitative analysis (increasing cycle numbers, as indicated). As shown, β-actin transcript was determined in parallel and used as a control. (B) Real-time PCR was employed to confirm hypoxia inducibility of CD39 in cultured endothelial cells (HMEC-1). Data were calculated relative to internal housekeeping gene (β-actin) and are expressed as fold increase over normoxia ± SD at each indicated time. Results are derived from three experiments in each condition. (C) Human saphenous vein was obtained from patients undergoing aorto-coronary bypass surgery and exposed ex vivo to ambient normoxia (pO 2 147 torr, 24 h) or hypoxia (pO 2 20 torr for 2, 8, or 24 h). After total RNA isolation, real-time PCR was performed to investigate CD39 inducibility by hypoxia. Data were calculated relative to internal control (β-actin) and are expressed as fold increase over normoxia ± SD at each indicated time. Results are derived from three experiments in each condition. (D) Increase in surface CD39 surface protein with hypoxic exposure. Confluent HMEC-1 monolayers were exposed to indicated periods of hypoxia, monolayers were washed, surface proteins were biotinylated, and cells were lysed. CD39 was immunoprecipitated with mAb directed against human CD39. Immunoprecipitates were resolved by SDS-PAGE, and resultant Western blots were probed with avidin-peroxidase. A representative experiment of three is shown. (E) Validation etheno-ATP (E-ATP) and etheno-AMP (E-AMP) resolution by HPLC. Shown is a representative tracing indicating resolution of definitive peaks at UV 260 nm. (F) Functional increase in CD39 surface activity by hypoxia. Endothelial monolayers were exposed to 48 h hypoxia or normoxia, washed, and surface CD39 activity was determined by HPLC analysis of E-ATP conversion to E-AMP in the presence of the CD73-inhibitor αβ-methylene-ADP (10 μM, to prevent further metabolism of E-AMP to E-adenosine). Data are derived from five to seven monolayers in each condition, and results are expressed as E-AMP: E-ATP ratio ± SD.

    Journal: The Journal of Experimental Medicine

    Article Title: Coordinated Adenine Nucleotide Phosphohydrolysis and Nucleoside Signaling in Posthypoxic Endothelium

    doi: 10.1084/jem.20030891

    Figure Lengend Snippet: Induction of functional CD39 is by hypoxia. (A) Confluent HMEC-1 monolayers were exposed to normoxia (pO 2 147 torr, 18 h) or hypoxia (pO 2 20 torr, 18 h). Total RNA was isolated, and CD39 mRNA levels were determined by RT-PCR using semiquantitative analysis (increasing cycle numbers, as indicated). As shown, β-actin transcript was determined in parallel and used as a control. (B) Real-time PCR was employed to confirm hypoxia inducibility of CD39 in cultured endothelial cells (HMEC-1). Data were calculated relative to internal housekeeping gene (β-actin) and are expressed as fold increase over normoxia ± SD at each indicated time. Results are derived from three experiments in each condition. (C) Human saphenous vein was obtained from patients undergoing aorto-coronary bypass surgery and exposed ex vivo to ambient normoxia (pO 2 147 torr, 24 h) or hypoxia (pO 2 20 torr for 2, 8, or 24 h). After total RNA isolation, real-time PCR was performed to investigate CD39 inducibility by hypoxia. Data were calculated relative to internal control (β-actin) and are expressed as fold increase over normoxia ± SD at each indicated time. Results are derived from three experiments in each condition. (D) Increase in surface CD39 surface protein with hypoxic exposure. Confluent HMEC-1 monolayers were exposed to indicated periods of hypoxia, monolayers were washed, surface proteins were biotinylated, and cells were lysed. CD39 was immunoprecipitated with mAb directed against human CD39. Immunoprecipitates were resolved by SDS-PAGE, and resultant Western blots were probed with avidin-peroxidase. A representative experiment of three is shown. (E) Validation etheno-ATP (E-ATP) and etheno-AMP (E-AMP) resolution by HPLC. Shown is a representative tracing indicating resolution of definitive peaks at UV 260 nm. (F) Functional increase in CD39 surface activity by hypoxia. Endothelial monolayers were exposed to 48 h hypoxia or normoxia, washed, and surface CD39 activity was determined by HPLC analysis of E-ATP conversion to E-AMP in the presence of the CD73-inhibitor αβ-methylene-ADP (10 μM, to prevent further metabolism of E-AMP to E-adenosine). Data are derived from five to seven monolayers in each condition, and results are expressed as E-AMP: E-ATP ratio ± SD.

    Article Snippet: Ratio of E-ATP (Molecular Probes Inc.) and E-AMP (Sigma-Aldrich) was measured with a 0–50% methanol/H2 O gradient mobile phase (2 ml/min over 10 min).

    Techniques: Functional Assay, Isolation, Reverse Transcription Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Cell Culture, Derivative Assay, Ex Vivo, Immunoprecipitation, SDS Page, Western Blot, Avidin-Biotin Assay, High Performance Liquid Chromatography, Activity Assay

    Role of CD39 in ATP-elicited changes of posthypoxic endothelial permeability. (A) HMEC-1 were loaded with CD39-specific siRNA, control ribonucleotide or mock treated (control) and exposed to hypoxia or normoxia (48 h). Monolayers were washed, surface protein was biotinylated, and cells were lysed. CD39 was immunoprecipitated and resolved by SDS-PAGE, and resultant Western blots were probed with avidin-peroxidase. As a control for specificity, CD73 protein induction by hypoxia was assessed in parallel. (B) Influence of CD39 suppression by siRNA on functional surface protein. HMEC-1 were loaded with CD39-specific siRNA, control-ribonucleotide, or mock treated and CD39 activity was determined by HPLC analysis of E-ATP conversion to E-AMP (in the presence of the CD73-inhibitor αβ-methylene-ADP). Data are derived from five to seven monolayers in each condition, and results are expressed as E-AMP: E-ATP ratio ± SD. (C) Influence of CD39 suppression by siRNA on endothelial barrier. HMEC-1 were loaded with CD39-specific siRNA, control ribonucleotide or mock treated (control), exposed to hypoxia or normoxia (48 h), and permeability to 70 kD FITC in the presence or absence of ATP (100 μM) was assessed (*, P

    Journal: The Journal of Experimental Medicine

    Article Title: Coordinated Adenine Nucleotide Phosphohydrolysis and Nucleoside Signaling in Posthypoxic Endothelium

    doi: 10.1084/jem.20030891

    Figure Lengend Snippet: Role of CD39 in ATP-elicited changes of posthypoxic endothelial permeability. (A) HMEC-1 were loaded with CD39-specific siRNA, control ribonucleotide or mock treated (control) and exposed to hypoxia or normoxia (48 h). Monolayers were washed, surface protein was biotinylated, and cells were lysed. CD39 was immunoprecipitated and resolved by SDS-PAGE, and resultant Western blots were probed with avidin-peroxidase. As a control for specificity, CD73 protein induction by hypoxia was assessed in parallel. (B) Influence of CD39 suppression by siRNA on functional surface protein. HMEC-1 were loaded with CD39-specific siRNA, control-ribonucleotide, or mock treated and CD39 activity was determined by HPLC analysis of E-ATP conversion to E-AMP (in the presence of the CD73-inhibitor αβ-methylene-ADP). Data are derived from five to seven monolayers in each condition, and results are expressed as E-AMP: E-ATP ratio ± SD. (C) Influence of CD39 suppression by siRNA on endothelial barrier. HMEC-1 were loaded with CD39-specific siRNA, control ribonucleotide or mock treated (control), exposed to hypoxia or normoxia (48 h), and permeability to 70 kD FITC in the presence or absence of ATP (100 μM) was assessed (*, P

    Article Snippet: Ratio of E-ATP (Molecular Probes Inc.) and E-AMP (Sigma-Aldrich) was measured with a 0–50% methanol/H2 O gradient mobile phase (2 ml/min over 10 min).

    Techniques: Permeability, Immunoprecipitation, SDS Page, Western Blot, Avidin-Biotin Assay, Functional Assay, Activity Assay, High Performance Liquid Chromatography, Derivative Assay

    HNK affect energy homeostasis of cancer cells by effecting cellular ATP. (a) Linear standard curves were generated using pure standards of ATP and ADP with R 2 value greater than 0.99 for both ATP and ADP for HPLC. (b) ATP modulation by HNK in B16/F-10 and SKMEL-28 as measured by in a Luciferase based assay. No changes in ATP levels were seen at 24 h, but a statistically significant decrease in ATP levels was seen only in SKMEL-28 cells at 48 h. (c) HPLC was used to evaluate ATP and ADP levels in both cells lines at 24 and 48 h. No changes were seen at 24 h, but ATP levels were statistically lower in both cell lines at 48 h. No changes in ADP levels were observed.

    Journal: American journal of surgery

    Article Title: Honokiol affects melanoma cell growth by targeting the AMPK signaling pathway

    doi: 10.1016/j.amjsurg.2014.09.014

    Figure Lengend Snippet: HNK affect energy homeostasis of cancer cells by effecting cellular ATP. (a) Linear standard curves were generated using pure standards of ATP and ADP with R 2 value greater than 0.99 for both ATP and ADP for HPLC. (b) ATP modulation by HNK in B16/F-10 and SKMEL-28 as measured by in a Luciferase based assay. No changes in ATP levels were seen at 24 h, but a statistically significant decrease in ATP levels was seen only in SKMEL-28 cells at 48 h. (c) HPLC was used to evaluate ATP and ADP levels in both cells lines at 24 and 48 h. No changes were seen at 24 h, but ATP levels were statistically lower in both cell lines at 48 h. No changes in ADP levels were observed.

    Article Snippet: Cellular ATP pool in melanoma cells after HNK treatment was determined by a Luminescence based ATP quantification assay kit (Invitrogen) based on previously published protocol ( ).

    Techniques: Generated, High Performance Liquid Chromatography, Luciferase

    Kinase profiling of U2OS cell line in the presence of circadian period–lengthening compounds. (A) Chemical structure and mechanism of the desthiobiotin-ATP probe for kinase active-site labelling. Desthiobiotin-ATP probes are nucleotide derivatives, which covalently modify the active site of enzymes (kinases) with conserved lysine residues in the nucleotide-binding site. The structure of desthiobiotin-ATP consists of a modified biotin attached to the nucleotide by a labile acyl-phosphate bond. (B) Enrichment efficiency of desthiobiotin-ATP labelled (active-site labelled) peptides in quantitative mass spectrometric analysis (n = 3). (C) Kinome profile of U2OS cell line after treatment with circadian period–lengthening compounds (25 μM). Heat map representation of relative abundance (against DMSO control) of 174 kinases in longdaysin-, purvalanol A–, roscovitine-, and SP600125-treated cell lysates (mean, n = 3). Relative expression and inhibition of kinases were measured using TMT-based quantitation by MS.

    Journal: Life Science Alliance

    Article Title: Phenotypic proteomic profiling identifies a landscape of targets for circadian clock–modulating compounds

    doi: 10.26508/lsa.201900603

    Figure Lengend Snippet: Kinase profiling of U2OS cell line in the presence of circadian period–lengthening compounds. (A) Chemical structure and mechanism of the desthiobiotin-ATP probe for kinase active-site labelling. Desthiobiotin-ATP probes are nucleotide derivatives, which covalently modify the active site of enzymes (kinases) with conserved lysine residues in the nucleotide-binding site. The structure of desthiobiotin-ATP consists of a modified biotin attached to the nucleotide by a labile acyl-phosphate bond. (B) Enrichment efficiency of desthiobiotin-ATP labelled (active-site labelled) peptides in quantitative mass spectrometric analysis (n = 3). (C) Kinome profile of U2OS cell line after treatment with circadian period–lengthening compounds (25 μM). Heat map representation of relative abundance (against DMSO control) of 174 kinases in longdaysin-, purvalanol A–, roscovitine-, and SP600125-treated cell lysates (mean, n = 3). Relative expression and inhibition of kinases were measured using TMT-based quantitation by MS.

    Article Snippet: Compound-treated and control cell lysates were labelled with 5 μM of desthiobiotin-ATP (Thermo Fisher Scientific) for 10 min at room temperature.

    Techniques: Binding Assay, Modification, Expressing, Inhibition, Quantitation Assay, Mass Spectrometry

    Circadian period–altering compounds inhibit several kinases in the mammalian system. (A) Schematic representation showing kinase profiling of U2OS cell line in the presence of circadian period–lengthening compounds (longdaysin, purvalanol A, roscovitine, and SP600125) by using desthiobiotin nucleotide probes in combination with TMT-based quantitative proteomics (biological replicates, n = 3). This workflow allows targeted capture and quantification of kinases using the active-site probes (desthiobiotin-ATP). (B) Venn diagram representing the overlap between the kinases inhibited ( p Adj

    Journal: Life Science Alliance

    Article Title: Phenotypic proteomic profiling identifies a landscape of targets for circadian clock–modulating compounds

    doi: 10.26508/lsa.201900603

    Figure Lengend Snippet: Circadian period–altering compounds inhibit several kinases in the mammalian system. (A) Schematic representation showing kinase profiling of U2OS cell line in the presence of circadian period–lengthening compounds (longdaysin, purvalanol A, roscovitine, and SP600125) by using desthiobiotin nucleotide probes in combination with TMT-based quantitative proteomics (biological replicates, n = 3). This workflow allows targeted capture and quantification of kinases using the active-site probes (desthiobiotin-ATP). (B) Venn diagram representing the overlap between the kinases inhibited ( p Adj

    Article Snippet: Compound-treated and control cell lysates were labelled with 5 μM of desthiobiotin-ATP (Thermo Fisher Scientific) for 10 min at room temperature.

    Techniques:

    Semapimod abrogates modification of gp96 with ATP-desthiobiotin. (A) Silver-stained gel of IEC-6 proteins modified by ATP-desthiobiotin and collected on streptavidin-agarose. Cells were pre-treated with 10 μM Semapimod for 15 min and then treated

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Experimental anti-inflammatory drug Semapimod inhibits Toll-like receptor signaling by targeting the TLR chaperone gp96

    doi: 10.4049/jimmunol.1502135

    Figure Lengend Snippet: Semapimod abrogates modification of gp96 with ATP-desthiobiotin. (A) Silver-stained gel of IEC-6 proteins modified by ATP-desthiobiotin and collected on streptavidin-agarose. Cells were pre-treated with 10 μM Semapimod for 15 min and then treated

    Article Snippet: Reagents were purchased from the following suppliers: Semapimod, Medkoo Biosciences (Chapel Hill, NC); recombinant canine gp96, catalog # ADI-SPP-766, Enzo Life Sciences (Farmingdale, NY); recombinant human HSP90, catalog # SPR-101A, StressMarq Biosciences, Victoria BC, Canada; LPS from E. coli 0127:B8, MG132, geldanamycin, radicicol, and NECA, Sigma Aldrich (St. Louis, MO); tripalmytoil – cysteine-serine-(lysine)4 (Pam3 CSK4 ) , Tocris Bioscience (Bristol, UK); ultrapure flagellin from S. typhimurium , InvivoGen (San Diego, CA), recombinant rat IL-1β, Peprotech (Rocky Hill, NJ), peroxynitrite, Cayman Chemical (Ann Arbor, MI) ATP-desthiobiotin kit, Thermo Scientific (Rockford, IL).

    Techniques: Modification, Staining

    MN induced mitochondrial dysfunction. HCEnC, HCECi, and FECDi cells were exposed to MN in a dose-dependent manner for 1 h with or without NAC pretreatment and collected for measurement of mitochondrial ROS production by flow cytometry, ATP levels,

    Journal: Antioxidants & Redox Signaling

    Article Title: Menadione-Induced DNA Damage Leads to Mitochondrial Dysfunction and Fragmentation During Rosette Formation in Fuchs Endothelial Corneal Dystrophy

    doi: 10.1089/ars.2015.6532

    Figure Lengend Snippet: MN induced mitochondrial dysfunction. HCEnC, HCECi, and FECDi cells were exposed to MN in a dose-dependent manner for 1 h with or without NAC pretreatment and collected for measurement of mitochondrial ROS production by flow cytometry, ATP levels,

    Article Snippet: After determining cellular viability, cells were resuspended in 100 μl warm HBSS and transferred to a 96-well plate, and ATP content was determined with recombinant firefly luciferase and D-luciferin in all three cell lines (HCEnC-21T, HCECi, and FECDi) using a bioluminescence ATP Determination Assay Kit (Molecular Probes).

    Techniques: Flow Cytometry, Cytometry