at2 r rabbit polyclonal antibody Search Results


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  • 94
    Alomone Labs aar 012
    Angiotensin II AT 2 receptor immunocytochemistry in the mouse brain. (A) and (B) Antibody 2818-1 from Epitomics (dilution 1:400). The AT 2 receptor antibody reacts with parenchymal microvessels (indicated with arrowheads in A and B, and in box in panel A) in the cerebral motor cortex from -1.22 mm to Bregma, and the immunocytochemistry is similar in wild-type (WT) (A) and AT 2 knock-out (KO) mice (B). (C) and (D) Antibody sc-9040 from Santa Cruz (dilution 1:2000). The antibody detects ciliated ependymal cells (arrowheads) of the lateral ventricle (V), and the staining is similar in the wild type (C) or knockout mice (D) located at the same coordinates as A and B. (E) and (F): antibody <t>AAR-012</t> from Alomone (dilution 1:3000). In the cerebral motor cortex, the antibody detects cell colocalization with the neuronal marker NeuN (indicated with arrowheads in E and F and in the boxes in panel E). The staining is similar in wild-type (E) and AT 2 receptor knockout (F) mice. Scale bar = 20 µm.
    Aar 012, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aar 012/product/Alomone Labs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    aar 012 - by Bioz Stars, 2022-09
    94/100 stars
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    95
    Santa Cruz Biotechnology anti at2r rabbit polyclonal antibody
    Angiotensin II AT 2 receptor immunocytochemistry in the mouse brain. (A) and (B) Antibody 2818-1 from Epitomics (dilution 1:400). The AT 2 receptor antibody reacts with parenchymal microvessels (indicated with arrowheads in A and B, and in box in panel A) in the cerebral motor cortex from -1.22 mm to Bregma, and the immunocytochemistry is similar in wild-type (WT) (A) and AT 2 knock-out (KO) mice (B). (C) and (D) Antibody sc-9040 from Santa Cruz (dilution 1:2000). The antibody detects ciliated ependymal cells (arrowheads) of the lateral ventricle (V), and the staining is similar in the wild type (C) or knockout mice (D) located at the same coordinates as A and B. (E) and (F): antibody <t>AAR-012</t> from Alomone (dilution 1:3000). In the cerebral motor cortex, the antibody detects cell colocalization with the neuronal marker NeuN (indicated with arrowheads in E and F and in the boxes in panel E). The staining is similar in wild-type (E) and AT 2 receptor knockout (F) mice. Scale bar = 20 µm.
    Anti At2r Rabbit Polyclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti at2r rabbit polyclonal antibody/product/Santa Cruz Biotechnology
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti at2r rabbit polyclonal antibody - by Bioz Stars, 2022-09
    95/100 stars
      Buy from Supplier

    94
    Novus Biologicals at2r
    Effects of Ang(1-7) and/or A-779 treatments to the sham and OVX animals for 6 weeks on the expressions of RAS different proteins and osteoclastogenesis modulating factors in the femoral bone heads. ( A ) Western blot analysis bands showing the expressions of AngII, Ang(1-7), AT1R, <t>AT2R,</t> ACE, ACE-2, MasR, RANKL and OPG. ( B ) Quantification of the scanning densitometry of the western blot bands (n = 8/group) expressed as arbitrary units. One-way ANOVA test followed by post hoc Student-Newman-Keuls multiple comparisons test were used for the statistical analysis. Columns and bars represent the mean ± SEM of each group. Statistical significance was considered when *P
    At2r, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/at2r/product/Novus Biologicals
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    at2r - by Bioz Stars, 2022-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Angiotensin II AT 2 receptor immunocytochemistry in the mouse brain. (A) and (B) Antibody 2818-1 from Epitomics (dilution 1:400). The AT 2 receptor antibody reacts with parenchymal microvessels (indicated with arrowheads in A and B, and in box in panel A) in the cerebral motor cortex from -1.22 mm to Bregma, and the immunocytochemistry is similar in wild-type (WT) (A) and AT 2 knock-out (KO) mice (B). (C) and (D) Antibody sc-9040 from Santa Cruz (dilution 1:2000). The antibody detects ciliated ependymal cells (arrowheads) of the lateral ventricle (V), and the staining is similar in the wild type (C) or knockout mice (D) located at the same coordinates as A and B. (E) and (F): antibody AAR-012 from Alomone (dilution 1:3000). In the cerebral motor cortex, the antibody detects cell colocalization with the neuronal marker NeuN (indicated with arrowheads in E and F and in the boxes in panel E). The staining is similar in wild-type (E) and AT 2 receptor knockout (F) mice. Scale bar = 20 µm.

    Journal: PLoS ONE

    Article Title: Commercially Available Angiotensin II At2 Receptor Antibodies Are Nonspecific

    doi: 10.1371/journal.pone.0069234

    Figure Lengend Snippet: Angiotensin II AT 2 receptor immunocytochemistry in the mouse brain. (A) and (B) Antibody 2818-1 from Epitomics (dilution 1:400). The AT 2 receptor antibody reacts with parenchymal microvessels (indicated with arrowheads in A and B, and in box in panel A) in the cerebral motor cortex from -1.22 mm to Bregma, and the immunocytochemistry is similar in wild-type (WT) (A) and AT 2 knock-out (KO) mice (B). (C) and (D) Antibody sc-9040 from Santa Cruz (dilution 1:2000). The antibody detects ciliated ependymal cells (arrowheads) of the lateral ventricle (V), and the staining is similar in the wild type (C) or knockout mice (D) located at the same coordinates as A and B. (E) and (F): antibody AAR-012 from Alomone (dilution 1:3000). In the cerebral motor cortex, the antibody detects cell colocalization with the neuronal marker NeuN (indicated with arrowheads in E and F and in the boxes in panel E). The staining is similar in wild-type (E) and AT 2 receptor knockout (F) mice. Scale bar = 20 µm.

    Article Snippet: One of the antibodies tested (AAR-012 from Alomone lab) was raised against amino acids 21-35, untranslated in AT2 knockout mice.

    Techniques: Immunocytochemistry, Knock-Out, Mouse Assay, Staining, Marker

    Regulation of VEGF, fibronectin and laminin β1 expression in kidneys from hyperglycemic AT2KO mice A. AT2 protein level was measured by immunoblot using a specific antibody. The lower panel shows combined data obtained on kidneys from 5 individual mice for each experimental condition. B. VEGF protein (B) and mRNA (C) levels were measured by immunoblot and RT-qPCR, respectively, on the same number of kidneys as in panel A. Identical experiments were performed for the protein and mRNA levels of fibronectin (D - E) and laminin β1 (F - G). **p

    Journal: Cellular signalling

    Article Title: Acute hyperglycemia rapidly stimulates VEGF mRNA translation in the kidney. Role of angiotensin type 2 receptor (AT2)

    doi: 10.1016/j.cellsig.2010.07.012

    Figure Lengend Snippet: Regulation of VEGF, fibronectin and laminin β1 expression in kidneys from hyperglycemic AT2KO mice A. AT2 protein level was measured by immunoblot using a specific antibody. The lower panel shows combined data obtained on kidneys from 5 individual mice for each experimental condition. B. VEGF protein (B) and mRNA (C) levels were measured by immunoblot and RT-qPCR, respectively, on the same number of kidneys as in panel A. Identical experiments were performed for the protein and mRNA levels of fibronectin (D - E) and laminin β1 (F - G). **p

    Article Snippet: Conformation-specific antibodies directed against activated AT1 and AT2 were from Assay Designs (Ann Arbor, MI), and antibodies directed against AT1 and AT2 were purchased from Alomone Labs (Jerusalem, Israel).

    Techniques: Expressing, Mouse Assay, Quantitative RT-PCR

    VEGF mRNA translation in hyperglycemic kidneys A. VEGF mRNA translation was studied by polysome assay. The shift of VEGF mRNA toward the heaviest fractions in diabetic kidneys indicates active translation, and is reversed by the AT2 but not the AT1 antagonist. Note that the distribution of GAPDH mRNA is unaffected by the various treatments. The figure is representative of experiments performed on kidneys from 3 individual mice. B. Association of hnRNP K with VEGF mRNA was studied by immunoprecipitation with anti-hnRNP K antibody followed by extraction of associated RNA associated and RT-PCR. Note that hnRNP K is constitutively associated with both VEGF and GAPDH mRNAs and that only the association with VEGF mRNA is increased in diabetic kidneys. The figure is representative of experiments performed on kidneys from 3 individual mice. **p

    Journal: Cellular signalling

    Article Title: Acute hyperglycemia rapidly stimulates VEGF mRNA translation in the kidney. Role of angiotensin type 2 receptor (AT2)

    doi: 10.1016/j.cellsig.2010.07.012

    Figure Lengend Snippet: VEGF mRNA translation in hyperglycemic kidneys A. VEGF mRNA translation was studied by polysome assay. The shift of VEGF mRNA toward the heaviest fractions in diabetic kidneys indicates active translation, and is reversed by the AT2 but not the AT1 antagonist. Note that the distribution of GAPDH mRNA is unaffected by the various treatments. The figure is representative of experiments performed on kidneys from 3 individual mice. B. Association of hnRNP K with VEGF mRNA was studied by immunoprecipitation with anti-hnRNP K antibody followed by extraction of associated RNA associated and RT-PCR. Note that hnRNP K is constitutively associated with both VEGF and GAPDH mRNAs and that only the association with VEGF mRNA is increased in diabetic kidneys. The figure is representative of experiments performed on kidneys from 3 individual mice. **p

    Article Snippet: Conformation-specific antibodies directed against activated AT1 and AT2 were from Assay Designs (Ann Arbor, MI), and antibodies directed against AT1 and AT2 were purchased from Alomone Labs (Jerusalem, Israel).

    Techniques: Mouse Assay, Immunoprecipitation, Reverse Transcription Polymerase Chain Reaction

    Angiotensin receptors are expressed in normal murine kidneys AT1 (A) and AT2 (B) were detected in kidney slices as described in the methods section. Arrows indicate localization of AT1 and AT2. Magnification = 20X. AT1 is strongly expressed in the apical and lateral membranes of the tubules (thin arrows), and in mesangial cells (thick arrow),.AT2 is expressed mostly in glomerular endothelial cells (thick arrow) and in the proximal tubules (thin arrow). The pictures are representative of kidneys from 2 individual mice. C) detection of AT1 and AT2 by western blot in kidney cortex homogenates using the same antibodies as in A and B.

    Journal: Cellular signalling

    Article Title: Acute hyperglycemia rapidly stimulates VEGF mRNA translation in the kidney. Role of angiotensin type 2 receptor (AT2)

    doi: 10.1016/j.cellsig.2010.07.012

    Figure Lengend Snippet: Angiotensin receptors are expressed in normal murine kidneys AT1 (A) and AT2 (B) were detected in kidney slices as described in the methods section. Arrows indicate localization of AT1 and AT2. Magnification = 20X. AT1 is strongly expressed in the apical and lateral membranes of the tubules (thin arrows), and in mesangial cells (thick arrow),.AT2 is expressed mostly in glomerular endothelial cells (thick arrow) and in the proximal tubules (thin arrow). The pictures are representative of kidneys from 2 individual mice. C) detection of AT1 and AT2 by western blot in kidney cortex homogenates using the same antibodies as in A and B.

    Article Snippet: Conformation-specific antibodies directed against activated AT1 and AT2 were from Assay Designs (Ann Arbor, MI), and antibodies directed against AT1 and AT2 were purchased from Alomone Labs (Jerusalem, Israel).

    Techniques: Mouse Assay, Western Blot

    Signaling pathways activated by AT1 and AT2 in diabetic kidneys The diagram depicts a summary of the data obtained in this study. AT1 activation by hyperglycemia activates mTOR independently of Akt and leads to inactivation of eEF2K but is not sufficient to cause the dephosphorylation and activation of eEF2. Activation of AT2 stimulates the Akt-mTOR pathway that inactivates eEF2K and activates PP2A that dephosphorylates eEF2; AT2 also mediates hyperglycemia-induced increment in eEF1A. Activation of eEF2 and up-regulation of eEF1A stimulate elongation phase of mRNA translation, leading to increased translation of VEGF mRNA.

    Journal: Cellular signalling

    Article Title: Acute hyperglycemia rapidly stimulates VEGF mRNA translation in the kidney. Role of angiotensin type 2 receptor (AT2)

    doi: 10.1016/j.cellsig.2010.07.012

    Figure Lengend Snippet: Signaling pathways activated by AT1 and AT2 in diabetic kidneys The diagram depicts a summary of the data obtained in this study. AT1 activation by hyperglycemia activates mTOR independently of Akt and leads to inactivation of eEF2K but is not sufficient to cause the dephosphorylation and activation of eEF2. Activation of AT2 stimulates the Akt-mTOR pathway that inactivates eEF2K and activates PP2A that dephosphorylates eEF2; AT2 also mediates hyperglycemia-induced increment in eEF1A. Activation of eEF2 and up-regulation of eEF1A stimulate elongation phase of mRNA translation, leading to increased translation of VEGF mRNA.

    Article Snippet: Conformation-specific antibodies directed against activated AT1 and AT2 were from Assay Designs (Ann Arbor, MI), and antibodies directed against AT1 and AT2 were purchased from Alomone Labs (Jerusalem, Israel).

    Techniques: Activation Assay, De-Phosphorylation Assay

    Effects of Ang(1-7) and/or A-779 treatments to the sham and OVX animals for 6 weeks on the expressions of RAS different proteins and osteoclastogenesis modulating factors in the femoral bone heads. ( A ) Western blot analysis bands showing the expressions of AngII, Ang(1-7), AT1R, AT2R, ACE, ACE-2, MasR, RANKL and OPG. ( B ) Quantification of the scanning densitometry of the western blot bands (n = 8/group) expressed as arbitrary units. One-way ANOVA test followed by post hoc Student-Newman-Keuls multiple comparisons test were used for the statistical analysis. Columns and bars represent the mean ± SEM of each group. Statistical significance was considered when *P

    Journal: Scientific Reports

    Article Title: Angiotensin (1-7) ameliorates the structural and biochemical alterations of ovariectomy-induced osteoporosis in rats via activation of ACE-2/Mas receptor axis

    doi: 10.1038/s41598-017-02570-x

    Figure Lengend Snippet: Effects of Ang(1-7) and/or A-779 treatments to the sham and OVX animals for 6 weeks on the expressions of RAS different proteins and osteoclastogenesis modulating factors in the femoral bone heads. ( A ) Western blot analysis bands showing the expressions of AngII, Ang(1-7), AT1R, AT2R, ACE, ACE-2, MasR, RANKL and OPG. ( B ) Quantification of the scanning densitometry of the western blot bands (n = 8/group) expressed as arbitrary units. One-way ANOVA test followed by post hoc Student-Newman-Keuls multiple comparisons test were used for the statistical analysis. Columns and bars represent the mean ± SEM of each group. Statistical significance was considered when *P

    Article Snippet: The primary antibodies of AngII (Cat #sc-9040), Ang(1-7) (Cat #sc-319824), Mas-receptor (Cat #sc-54848), RANKL (Cat #sc-9073) and OPG (Cat #sc-8468) were supplied from Santa Cruz Biotechnology, Inc. (Dallas, Texas, USA), while the primary antibodies for AT1R (Cat #NBP1-77078), AT2R (Cat #NBP1-77368), ACE (Cat #NBP1-19760), ACE-2 (Cat #NBP1-76614PEP) and the secondary antibody (Goat antirabbit IgG- HRP-1mg Goat mab) were purchased from Novus Biologicals (Littleton, Colorado, USA).

    Techniques: Western Blot