aspartate transaminase Search Results


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  • 99
    Millipore aspartate transaminase
    Aspartate Transaminase, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Randox aspartate aminotransferase
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    FUJIFILM aspartate aminotransferase
    Aspartate Aminotransferase, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 92/100, based on 366 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abbott Laboratories aspartate aminotransferase
    Aspartate Aminotransferase, supplied by Abbott Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 287 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Pointe Scientific aspartate aminotransferase
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    Siemens AG aspartate aminotransferase
    Aspartate Aminotransferase, supplied by Siemens AG, used in various techniques. Bioz Stars score: 92/100, based on 164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioSino aspartate aminotransferase
    Aspartate Aminotransferase, supplied by BioSino, used in various techniques. Bioz Stars score: 92/100, based on 174 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stanbio aspartate aminotransferase
    Aspartate Aminotransferase, supplied by Stanbio, used in various techniques. Bioz Stars score: 92/100, based on 130 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    International Federation of Clinical Chemistry and Laboratory Medicine aspartate aminotransferase
    Aspartate Aminotransferase, supplied by International Federation of Clinical Chemistry and Laboratory Medicine, used in various techniques. Bioz Stars score: 92/100, based on 113 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Teco Diagnostics aspartate aminotransferase
    Aspartate Aminotransferase, supplied by Teco Diagnostics, used in various techniques. Bioz Stars score: 92/100, based on 89 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    Abcam aspartate aminotransferase
    Aspartate Aminotransferase, supplied by Abcam, used in various techniques. Bioz Stars score: 98/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Dade Behring aspartate aminotransferase
    Aspartate Aminotransferase, supplied by Dade Behring, used in various techniques. Bioz Stars score: 92/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ERBA Diagnostics aspartate aminotransferase
    Aspartate Aminotransferase, supplied by ERBA Diagnostics, used in various techniques. Bioz Stars score: 92/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    BioVision aspartate aminotransferase ast or sgot activity colorimetric assay kit
    Aspartate Aminotransferase Ast Or Sgot Activity Colorimetric Assay Kit, supplied by BioVision, used in various techniques. Bioz Stars score: 97/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Catachem aspartate aminotransferase
    Aspartate Aminotransferase, supplied by Catachem, used in various techniques. Bioz Stars score: 92/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher aspartate aminotransferase
    Aspartate Aminotransferase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 303 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam aspartate aminotransferase activity assay kit
    Aspartate Aminotransferase Activity Assay Kit, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck KGaA aspartate aminotransferase
    Aspartate Aminotransferase, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Teco Diagnostics ast sgot reagent
    Ast Sgot Reagent, supplied by Teco Diagnostics, used in various techniques. Bioz Stars score: 88/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Cayman Chemical aspartate aminotransferase
    Aspartate Aminotransferase, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 95/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Sekisui Diagnostics aspartate aminotransferase
    Aspartate Aminotransferase, supplied by Sekisui Diagnostics, used in various techniques. Bioz Stars score: 92/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    got1  (Abcam)
    93
    Abcam got1
    p53 Sustains Aspartate Metabolism under Glutamine Deprivation (A) HCT116 WT and KO clones were cultured in glutamine-free medium for 4 days. Extracellular serine, alanine, and aspartate, normalized to cell number, was quantified over 24 hr. Data are presented as mean ± SEM of one representative experiment (averages of triplicate wells). (B) Isotope tracing of [ 15 N]aspartate into intracellular glutamate and glutamine. Metabolite percentages in glutamine-deprived HCT116 clones are represented as mean ± SEM of triplicate wells. (C) Isotope tracing of [U- 13 C, 15 N]alanine into intracellular glutamate and glutamine. Metabolite percentages in glutamine-deprived HCT116 clones are presented as mean ± SEM of triplicate wells. (D) Stable isotopomer tracing analysis of [U- 13 C]aspartate incorporation into TCA-cycle intermediates in HCT116 isogenic cells 5 days after glutamine starvation. Data are presented as mean ± SEM of one representative experiment (averages of triplicate wells). (E) Proliferation of HCT116 WT cells transiently depleted of <t>GOT1</t> and GOT2 using short interfering RNA (siRNA) and cultured in glutamine-free condition for 6 days. Data are presented as mean ± SEM of one representative experiment (averages of triplicate wells). The downregulation of these two enzymes was confirmed by western blot analysis (bottom panels). (F) Proliferation of HCT116 WT cells transiently depleted of GOT1 and GOT2 under fully fed conditions. Data are presented as mean ± SEM of one representative experiment (averages of triplicate wells). (G) Western blot analysis demonstrating the time course of GOT1 and GOT2 expression in HCT116 parental, WT1, and p53-KO1 clones grown in glutamine-free medium over 8 days. .
    Got1, supplied by Abcam, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Abcam anti aspartate aminotransferase
    p53 Sustains Aspartate Metabolism under Glutamine Deprivation (A) HCT116 WT and KO clones were cultured in glutamine-free medium for 4 days. Extracellular serine, alanine, and aspartate, normalized to cell number, was quantified over 24 hr. Data are presented as mean ± SEM of one representative experiment (averages of triplicate wells). (B) Isotope tracing of [ 15 N]aspartate into intracellular glutamate and glutamine. Metabolite percentages in glutamine-deprived HCT116 clones are represented as mean ± SEM of triplicate wells. (C) Isotope tracing of [U- 13 C, 15 N]alanine into intracellular glutamate and glutamine. Metabolite percentages in glutamine-deprived HCT116 clones are presented as mean ± SEM of triplicate wells. (D) Stable isotopomer tracing analysis of [U- 13 C]aspartate incorporation into TCA-cycle intermediates in HCT116 isogenic cells 5 days after glutamine starvation. Data are presented as mean ± SEM of one representative experiment (averages of triplicate wells). (E) Proliferation of HCT116 WT cells transiently depleted of <t>GOT1</t> and GOT2 using short interfering RNA (siRNA) and cultured in glutamine-free condition for 6 days. Data are presented as mean ± SEM of one representative experiment (averages of triplicate wells). The downregulation of these two enzymes was confirmed by western blot analysis (bottom panels). (F) Proliferation of HCT116 WT cells transiently depleted of GOT1 and GOT2 under fully fed conditions. Data are presented as mean ± SEM of one representative experiment (averages of triplicate wells). (G) Western blot analysis demonstrating the time course of GOT1 and GOT2 expression in HCT116 parental, WT1, and p53-KO1 clones grown in glutamine-free medium over 8 days. .
    Anti Aspartate Aminotransferase, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    N/A
    Sheep polyclonal antibody against Aspartate Transaminase conjugated to Biotin Isotype Note IgG Host Note Sheep Conjugation Note Biotin Application Note ELISA WB
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    Sheep polyclonal antibody against Aspartate Transaminase conjugated to Peroxidase Isotype Note IgG Host Note Sheep Conjugation Note Peroxidase Application Note ELISA WB
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    N/A
    Sheep Polyclonal antibody to Aspartate Transaminase Isotype Note Antiserum Host Note Sheep Conjugation Note Unconjugated Application Note ELISA WB
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    Image Search Results


    p53 Sustains Aspartate Metabolism under Glutamine Deprivation (A) HCT116 WT and KO clones were cultured in glutamine-free medium for 4 days. Extracellular serine, alanine, and aspartate, normalized to cell number, was quantified over 24 hr. Data are presented as mean ± SEM of one representative experiment (averages of triplicate wells). (B) Isotope tracing of [ 15 N]aspartate into intracellular glutamate and glutamine. Metabolite percentages in glutamine-deprived HCT116 clones are represented as mean ± SEM of triplicate wells. (C) Isotope tracing of [U- 13 C, 15 N]alanine into intracellular glutamate and glutamine. Metabolite percentages in glutamine-deprived HCT116 clones are presented as mean ± SEM of triplicate wells. (D) Stable isotopomer tracing analysis of [U- 13 C]aspartate incorporation into TCA-cycle intermediates in HCT116 isogenic cells 5 days after glutamine starvation. Data are presented as mean ± SEM of one representative experiment (averages of triplicate wells). (E) Proliferation of HCT116 WT cells transiently depleted of GOT1 and GOT2 using short interfering RNA (siRNA) and cultured in glutamine-free condition for 6 days. Data are presented as mean ± SEM of one representative experiment (averages of triplicate wells). The downregulation of these two enzymes was confirmed by western blot analysis (bottom panels). (F) Proliferation of HCT116 WT cells transiently depleted of GOT1 and GOT2 under fully fed conditions. Data are presented as mean ± SEM of one representative experiment (averages of triplicate wells). (G) Western blot analysis demonstrating the time course of GOT1 and GOT2 expression in HCT116 parental, WT1, and p53-KO1 clones grown in glutamine-free medium over 8 days. .

    Journal: Cell Metabolism

    Article Title: A Role for p53 in the Adaptation to Glutamine Starvation through the Expression of SLC1A3

    doi: 10.1016/j.cmet.2018.07.005

    Figure Lengend Snippet: p53 Sustains Aspartate Metabolism under Glutamine Deprivation (A) HCT116 WT and KO clones were cultured in glutamine-free medium for 4 days. Extracellular serine, alanine, and aspartate, normalized to cell number, was quantified over 24 hr. Data are presented as mean ± SEM of one representative experiment (averages of triplicate wells). (B) Isotope tracing of [ 15 N]aspartate into intracellular glutamate and glutamine. Metabolite percentages in glutamine-deprived HCT116 clones are represented as mean ± SEM of triplicate wells. (C) Isotope tracing of [U- 13 C, 15 N]alanine into intracellular glutamate and glutamine. Metabolite percentages in glutamine-deprived HCT116 clones are presented as mean ± SEM of triplicate wells. (D) Stable isotopomer tracing analysis of [U- 13 C]aspartate incorporation into TCA-cycle intermediates in HCT116 isogenic cells 5 days after glutamine starvation. Data are presented as mean ± SEM of one representative experiment (averages of triplicate wells). (E) Proliferation of HCT116 WT cells transiently depleted of GOT1 and GOT2 using short interfering RNA (siRNA) and cultured in glutamine-free condition for 6 days. Data are presented as mean ± SEM of one representative experiment (averages of triplicate wells). The downregulation of these two enzymes was confirmed by western blot analysis (bottom panels). (F) Proliferation of HCT116 WT cells transiently depleted of GOT1 and GOT2 under fully fed conditions. Data are presented as mean ± SEM of one representative experiment (averages of triplicate wells). (G) Western blot analysis demonstrating the time course of GOT1 and GOT2 expression in HCT116 parental, WT1, and p53-KO1 clones grown in glutamine-free medium over 8 days. .

    Article Snippet: Primary antibodies used were as follows: SLC1A3 (5685), AGC1 (64169), MDH2 (11908), phospho-p53 (S15) (9284) from Cell Signaling Technology; p53 (DO-1, sc-126), p21 (sc-397), ACTIN (sc-1616), AGC2 (sc-393303), MDM2 (SMP14, sc-965) from Santa Cruz Biotechnology; GOT1 (ab170950), GOT2 (ab90562), MDH1 (ab180152) from Abcam; glutamine synthetase (610517) from BD Transduction Laboratories.

    Techniques: Clone Assay, Cell Culture, Small Interfering RNA, Western Blot, Expressing

    Deletion of SLC1A3 Impedes the ETC and Phenocopies Depletion of the Mitochondrial Aspartate Transporters AGC1 and AGC2 under Glutamine Deprivation (A) Schematic representation of the malate-aspartate shuttle (MAS). In brief, the MAS is a system that allows the transfer of electrons from cytosolic NADH to produce mitochondrial NADH where it is oxidized in the ETC. In the cytoplasm MDH1 catalyzes the reduction of oxaloacetate (OAA), where it accepts an electron from NADH to produce malate and NAD + . Malate can then enter the mitochondria where it is oxidized by MDH2 to OAA, resulting in the formation of mitochondrial NADH. Mitochondrial OAA is transaminated into aspartate by GOT2 whereby aspartate exits the mitochondria in exchange for cytosolic glutamate through a carrier. OAA is recovered in the cytosol by GOT1. By coupling aspartate-glutamate exchange, the aspartate-glutamate carrier is essential for the shuttle and is thought to represent the rate-limiting step. (B) Respiratory profiles of HCT116 WT cells transiently depleted of SLC1A3 and grown for 24 hr in glutamine-free medium, in the presence of mitochondrial inhibitors (oligomycin, FCCP [carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone] antimycin A/rotenone). Arrows indicate incubation of cells with the indicated inhibitors. Data are presented as mean ± SEM of one representative experiment (n = 6 wells). (C) Oxygen consumption rates (OCR) of HCT116 WT and p53-null clones 2 days after glutamine deprivation as in (B). Data are presented as mean ± SEM of one representative experiment (n = 6 wells). (D) Proliferation of HCT116 WT cells transiently depleted of AGC1 or AGC2 using siRNA and cultured in glutamine-free condition (LHS) or complete medium (RHS). Data are presented as mean ± SEM of one representative experiment (averages of triplicate wells). The downregulation of these two proteins was confirmed by western blot (middle panel). (E) HCT116 WT cells transiently depleted of AGC1 or AGC2 using siRNA were grown for 2 days in glutamine-free medium and pulsed with [U- 13 C]aspartate for the final 24 hr. Extracellular levels of aspartate (m+4), alanine, and serine normalized to cell number were quantified over 24 hr. Data are presented as mean ± SEM of one representative experiment (averages of triplicate wells). (F and G) HCT116 WT cells transiently depleted of AGC1 or AGC2 using siRNA were grown for 2 days in glutamine-free medium and pulsed with [U- 13 C]aspartate for the final 24 hr. Intracellular TCA-cycle intermediates (F) and glutamate and glutamine levels (G) were measured. Data are presented as mean ± SEM of one representative experiment (averages of triplicate wells). .

    Journal: Cell Metabolism

    Article Title: A Role for p53 in the Adaptation to Glutamine Starvation through the Expression of SLC1A3

    doi: 10.1016/j.cmet.2018.07.005

    Figure Lengend Snippet: Deletion of SLC1A3 Impedes the ETC and Phenocopies Depletion of the Mitochondrial Aspartate Transporters AGC1 and AGC2 under Glutamine Deprivation (A) Schematic representation of the malate-aspartate shuttle (MAS). In brief, the MAS is a system that allows the transfer of electrons from cytosolic NADH to produce mitochondrial NADH where it is oxidized in the ETC. In the cytoplasm MDH1 catalyzes the reduction of oxaloacetate (OAA), where it accepts an electron from NADH to produce malate and NAD + . Malate can then enter the mitochondria where it is oxidized by MDH2 to OAA, resulting in the formation of mitochondrial NADH. Mitochondrial OAA is transaminated into aspartate by GOT2 whereby aspartate exits the mitochondria in exchange for cytosolic glutamate through a carrier. OAA is recovered in the cytosol by GOT1. By coupling aspartate-glutamate exchange, the aspartate-glutamate carrier is essential for the shuttle and is thought to represent the rate-limiting step. (B) Respiratory profiles of HCT116 WT cells transiently depleted of SLC1A3 and grown for 24 hr in glutamine-free medium, in the presence of mitochondrial inhibitors (oligomycin, FCCP [carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone] antimycin A/rotenone). Arrows indicate incubation of cells with the indicated inhibitors. Data are presented as mean ± SEM of one representative experiment (n = 6 wells). (C) Oxygen consumption rates (OCR) of HCT116 WT and p53-null clones 2 days after glutamine deprivation as in (B). Data are presented as mean ± SEM of one representative experiment (n = 6 wells). (D) Proliferation of HCT116 WT cells transiently depleted of AGC1 or AGC2 using siRNA and cultured in glutamine-free condition (LHS) or complete medium (RHS). Data are presented as mean ± SEM of one representative experiment (averages of triplicate wells). The downregulation of these two proteins was confirmed by western blot (middle panel). (E) HCT116 WT cells transiently depleted of AGC1 or AGC2 using siRNA were grown for 2 days in glutamine-free medium and pulsed with [U- 13 C]aspartate for the final 24 hr. Extracellular levels of aspartate (m+4), alanine, and serine normalized to cell number were quantified over 24 hr. Data are presented as mean ± SEM of one representative experiment (averages of triplicate wells). (F and G) HCT116 WT cells transiently depleted of AGC1 or AGC2 using siRNA were grown for 2 days in glutamine-free medium and pulsed with [U- 13 C]aspartate for the final 24 hr. Intracellular TCA-cycle intermediates (F) and glutamate and glutamine levels (G) were measured. Data are presented as mean ± SEM of one representative experiment (averages of triplicate wells). .

    Article Snippet: Primary antibodies used were as follows: SLC1A3 (5685), AGC1 (64169), MDH2 (11908), phospho-p53 (S15) (9284) from Cell Signaling Technology; p53 (DO-1, sc-126), p21 (sc-397), ACTIN (sc-1616), AGC2 (sc-393303), MDM2 (SMP14, sc-965) from Santa Cruz Biotechnology; GOT1 (ab170950), GOT2 (ab90562), MDH1 (ab180152) from Abcam; glutamine synthetase (610517) from BD Transduction Laboratories.

    Techniques: Incubation, Clone Assay, Cell Culture, Western Blot