aspartate aminotransferase Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Millipore sgot
    HSV-1 replication is inhibited by reducing flux from glucose toward pyrimidine nucleotide synthesis. ( A ) Schematic diagram of glucose flux to pyrimidine nucleotide biosynthesis. Red lines mark siRNA-targeted reactions catalyzed by pyruvate carboxylase (PC) and aspartate <t>transaminase</t> 2 (GOT2). (OAA: oxaloacetate, AKG: oxoglutarate, Gln: glutamine). ( B ) RNA interference knockdown of pyruvate carboxylase (marked by arrow) in MRC5 cells. Cells were transfected with non-targeting siRNAs (NT) or siRNAs targeting pyruvate carboxylase (PC) and harvested at indicated time points after transfection. Pyruvate carboxylase levels in the cells were detected by western blot using specific antibodies. Beta-actin was employed as a loading control. ( C ) Buildup of 13 C 3 -labeled malate after switching MRC5 cells to uniformly 13 C-labeled glucose medium for 2 hours at 10 hpi of HSV-1 (F) infection. The cells have been transfected with a universal non-targeting siRNA (NT) or an siRNA targeting pyruvate carboxylase (PC) 120 h prior to infection (significance: p = 0.007). Symbols indicate experimental data points ±1 s.d.; n = 3; values are given in arbitrary units. ( D ) Production of infectious HSV-1 (F) and HCMV (AD169) virions in cells transfected with siRNAs against pyruvate carboxylase (PC), aspartate transaminase 2 (GOT2), or a universal negative control (NT). The transfection and infection of MRC5 cells were performed as described in Materials and Methods . Values are expressed relative to non-targeting control (±1 s.d.; n = 3). Conditions resulting in significantly altered virus production (p≤0.05) compared to treatment with the universal negative control are marked with a star.
    Sgot, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sgot/product/Millipore
    Average 97 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    sgot - by Bioz Stars, 2020-08
    97/100 stars
      Buy from Supplier

    88
    Roche aspartate aminotransferase serum glutamic oxaloacetic transaminase ast sgot
    HSV-1 replication is inhibited by reducing flux from glucose toward pyrimidine nucleotide synthesis. ( A ) Schematic diagram of glucose flux to pyrimidine nucleotide biosynthesis. Red lines mark siRNA-targeted reactions catalyzed by pyruvate carboxylase (PC) and aspartate <t>transaminase</t> 2 (GOT2). (OAA: oxaloacetate, AKG: oxoglutarate, Gln: glutamine). ( B ) RNA interference knockdown of pyruvate carboxylase (marked by arrow) in MRC5 cells. Cells were transfected with non-targeting siRNAs (NT) or siRNAs targeting pyruvate carboxylase (PC) and harvested at indicated time points after transfection. Pyruvate carboxylase levels in the cells were detected by western blot using specific antibodies. Beta-actin was employed as a loading control. ( C ) Buildup of 13 C 3 -labeled malate after switching MRC5 cells to uniformly 13 C-labeled glucose medium for 2 hours at 10 hpi of HSV-1 (F) infection. The cells have been transfected with a universal non-targeting siRNA (NT) or an siRNA targeting pyruvate carboxylase (PC) 120 h prior to infection (significance: p = 0.007). Symbols indicate experimental data points ±1 s.d.; n = 3; values are given in arbitrary units. ( D ) Production of infectious HSV-1 (F) and HCMV (AD169) virions in cells transfected with siRNAs against pyruvate carboxylase (PC), aspartate transaminase 2 (GOT2), or a universal negative control (NT). The transfection and infection of MRC5 cells were performed as described in Materials and Methods . Values are expressed relative to non-targeting control (±1 s.d.; n = 3). Conditions resulting in significantly altered virus production (p≤0.05) compared to treatment with the universal negative control are marked with a star.
    Aspartate Aminotransferase Serum Glutamic Oxaloacetic Transaminase Ast Sgot, supplied by Roche, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aspartate aminotransferase serum glutamic oxaloacetic transaminase ast sgot/product/Roche
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    aspartate aminotransferase serum glutamic oxaloacetic transaminase ast sgot - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    92
    Teco Diagnostics aspartate aminotransferase ast sgot reagent kit
    HSV-1 replication is inhibited by reducing flux from glucose toward pyrimidine nucleotide synthesis. ( A ) Schematic diagram of glucose flux to pyrimidine nucleotide biosynthesis. Red lines mark siRNA-targeted reactions catalyzed by pyruvate carboxylase (PC) and aspartate <t>transaminase</t> 2 (GOT2). (OAA: oxaloacetate, AKG: oxoglutarate, Gln: glutamine). ( B ) RNA interference knockdown of pyruvate carboxylase (marked by arrow) in MRC5 cells. Cells were transfected with non-targeting siRNAs (NT) or siRNAs targeting pyruvate carboxylase (PC) and harvested at indicated time points after transfection. Pyruvate carboxylase levels in the cells were detected by western blot using specific antibodies. Beta-actin was employed as a loading control. ( C ) Buildup of 13 C 3 -labeled malate after switching MRC5 cells to uniformly 13 C-labeled glucose medium for 2 hours at 10 hpi of HSV-1 (F) infection. The cells have been transfected with a universal non-targeting siRNA (NT) or an siRNA targeting pyruvate carboxylase (PC) 120 h prior to infection (significance: p = 0.007). Symbols indicate experimental data points ±1 s.d.; n = 3; values are given in arbitrary units. ( D ) Production of infectious HSV-1 (F) and HCMV (AD169) virions in cells transfected with siRNAs against pyruvate carboxylase (PC), aspartate transaminase 2 (GOT2), or a universal negative control (NT). The transfection and infection of MRC5 cells were performed as described in Materials and Methods . Values are expressed relative to non-targeting control (±1 s.d.; n = 3). Conditions resulting in significantly altered virus production (p≤0.05) compared to treatment with the universal negative control are marked with a star.
    Aspartate Aminotransferase Ast Sgot Reagent Kit, supplied by Teco Diagnostics, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aspartate aminotransferase ast sgot reagent kit/product/Teco Diagnostics
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    aspartate aminotransferase ast sgot reagent kit - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    84
    Boehringer Mannheim glutamic oxaloacetic transaminase got aspartate aminotransferase
    HSV-1 replication is inhibited by reducing flux from glucose toward pyrimidine nucleotide synthesis. ( A ) Schematic diagram of glucose flux to pyrimidine nucleotide biosynthesis. Red lines mark siRNA-targeted reactions catalyzed by pyruvate carboxylase (PC) and aspartate <t>transaminase</t> 2 (GOT2). (OAA: oxaloacetate, AKG: oxoglutarate, Gln: glutamine). ( B ) RNA interference knockdown of pyruvate carboxylase (marked by arrow) in MRC5 cells. Cells were transfected with non-targeting siRNAs (NT) or siRNAs targeting pyruvate carboxylase (PC) and harvested at indicated time points after transfection. Pyruvate carboxylase levels in the cells were detected by western blot using specific antibodies. Beta-actin was employed as a loading control. ( C ) Buildup of 13 C 3 -labeled malate after switching MRC5 cells to uniformly 13 C-labeled glucose medium for 2 hours at 10 hpi of HSV-1 (F) infection. The cells have been transfected with a universal non-targeting siRNA (NT) or an siRNA targeting pyruvate carboxylase (PC) 120 h prior to infection (significance: p = 0.007). Symbols indicate experimental data points ±1 s.d.; n = 3; values are given in arbitrary units. ( D ) Production of infectious HSV-1 (F) and HCMV (AD169) virions in cells transfected with siRNAs against pyruvate carboxylase (PC), aspartate transaminase 2 (GOT2), or a universal negative control (NT). The transfection and infection of MRC5 cells were performed as described in Materials and Methods . Values are expressed relative to non-targeting control (±1 s.d.; n = 3). Conditions resulting in significantly altered virus production (p≤0.05) compared to treatment with the universal negative control are marked with a star.
    Glutamic Oxaloacetic Transaminase Got Aspartate Aminotransferase, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/glutamic oxaloacetic transaminase got aspartate aminotransferase/product/Boehringer Mannheim
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    glutamic oxaloacetic transaminase got aspartate aminotransferase - by Bioz Stars, 2020-08
    84/100 stars
      Buy from Supplier

    93
    BioVision aspartate aminotransferase
    Effects of hypothermal stress on the mitochondria in the livers of freshwater (FW) and seawater (SW) milkfish. <t>AST,</t> aspartate transaminase; CS, citrate synthase; GDH, glutamate dehydrogenase; GLU, glutamate; α-KG, alpha-ketoglutarate; OAA, oxaloacetate.
    Aspartate Aminotransferase, supplied by BioVision, used in various techniques. Bioz Stars score: 93/100, based on 100 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aspartate aminotransferase/product/BioVision
    Average 93 stars, based on 100 article reviews
    Price from $9.99 to $1999.99
    aspartate aminotransferase - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    95
    Millipore aspartate aminotransferase activity assay alanine transaminase alt
    Effects of hypothermal stress on the mitochondria in the livers of freshwater (FW) and seawater (SW) milkfish. <t>AST,</t> aspartate transaminase; CS, citrate synthase; GDH, glutamate dehydrogenase; GLU, glutamate; α-KG, alpha-ketoglutarate; OAA, oxaloacetate.
    Aspartate Aminotransferase Activity Assay Alanine Transaminase Alt, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aspartate aminotransferase activity assay alanine transaminase alt/product/Millipore
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    aspartate aminotransferase activity assay alanine transaminase alt - by Bioz Stars, 2020-08
    95/100 stars
      Buy from Supplier

    92
    Bioo Scientific aspartate transaminase
    Effects of hypothermal stress on the mitochondria in the livers of freshwater (FW) and seawater (SW) milkfish. <t>AST,</t> aspartate transaminase; CS, citrate synthase; GDH, glutamate dehydrogenase; GLU, glutamate; α-KG, alpha-ketoglutarate; OAA, oxaloacetate.
    Aspartate Transaminase, supplied by Bioo Scientific, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aspartate transaminase/product/Bioo Scientific
    Average 92 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    aspartate transaminase - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    94
    Millipore aspartate aminotransferase activity assay kit
    Effects of hypothermal stress on the mitochondria in the livers of freshwater (FW) and seawater (SW) milkfish. <t>AST,</t> aspartate transaminase; CS, citrate synthase; GDH, glutamate dehydrogenase; GLU, glutamate; α-KG, alpha-ketoglutarate; OAA, oxaloacetate.
    Aspartate Aminotransferase Activity Assay Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aspartate aminotransferase activity assay kit/product/Millipore
    Average 94 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    aspartate aminotransferase activity assay kit - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    92
    Fisher Scientific aspartate aminotransferase
    Effects of hypothermal stress on the mitochondria in the livers of freshwater (FW) and seawater (SW) milkfish. <t>AST,</t> aspartate transaminase; CS, citrate synthase; GDH, glutamate dehydrogenase; GLU, glutamate; α-KG, alpha-ketoglutarate; OAA, oxaloacetate.
    Aspartate Aminotransferase, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aspartate aminotransferase/product/Fisher Scientific
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    aspartate aminotransferase - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    92
    rdi research diagnostics aspartate aminotransferase
    Effects of hypothermal stress on the mitochondria in the livers of freshwater (FW) and seawater (SW) milkfish. <t>AST,</t> aspartate transaminase; CS, citrate synthase; GDH, glutamate dehydrogenase; GLU, glutamate; α-KG, alpha-ketoglutarate; OAA, oxaloacetate.
    Aspartate Aminotransferase, supplied by rdi research diagnostics, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aspartate aminotransferase/product/rdi research diagnostics
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    aspartate aminotransferase - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    93
    Randox aspartate aminotransferase
    Effects of hypothermal stress on the mitochondria in the livers of freshwater (FW) and seawater (SW) milkfish. <t>AST,</t> aspartate transaminase; CS, citrate synthase; GDH, glutamate dehydrogenase; GLU, glutamate; α-KG, alpha-ketoglutarate; OAA, oxaloacetate.
    Aspartate Aminotransferase, supplied by Randox, used in various techniques. Bioz Stars score: 93/100, based on 353 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aspartate aminotransferase/product/Randox
    Average 93 stars, based on 353 article reviews
    Price from $9.99 to $1999.99
    aspartate aminotransferase - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    93
    Beckman Coulter aspartate aminotransferases ast sgot activities
    Effects of hypothermal stress on the mitochondria in the livers of freshwater (FW) and seawater (SW) milkfish. <t>AST,</t> aspartate transaminase; CS, citrate synthase; GDH, glutamate dehydrogenase; GLU, glutamate; α-KG, alpha-ketoglutarate; OAA, oxaloacetate.
    Aspartate Aminotransferases Ast Sgot Activities, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aspartate aminotransferases ast sgot activities/product/Beckman Coulter
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    aspartate aminotransferases ast sgot activities - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    92
    Siemens AG aspartate aminotransferase
    Effects of hypothermal stress on the mitochondria in the livers of freshwater (FW) and seawater (SW) milkfish. <t>AST,</t> aspartate transaminase; CS, citrate synthase; GDH, glutamate dehydrogenase; GLU, glutamate; α-KG, alpha-ketoglutarate; OAA, oxaloacetate.
    Aspartate Aminotransferase, supplied by Siemens AG, used in various techniques. Bioz Stars score: 92/100, based on 164 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aspartate aminotransferase/product/Siemens AG
    Average 92 stars, based on 164 article reviews
    Price from $9.99 to $1999.99
    aspartate aminotransferase - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    94
    Stanbio aspartate aminotransferase
    Effects of hypothermal stress on the mitochondria in the livers of freshwater (FW) and seawater (SW) milkfish. <t>AST,</t> aspartate transaminase; CS, citrate synthase; GDH, glutamate dehydrogenase; GLU, glutamate; α-KG, alpha-ketoglutarate; OAA, oxaloacetate.
    Aspartate Aminotransferase, supplied by Stanbio, used in various techniques. Bioz Stars score: 94/100, based on 130 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aspartate aminotransferase/product/Stanbio
    Average 94 stars, based on 130 article reviews
    Price from $9.99 to $1999.99
    aspartate aminotransferase - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    93
    Ningbo Purebio Biotechnology aspartate aminotransferase
    Effects of hypothermal stress on the mitochondria in the livers of freshwater (FW) and seawater (SW) milkfish. <t>AST,</t> aspartate transaminase; CS, citrate synthase; GDH, glutamate dehydrogenase; GLU, glutamate; α-KG, alpha-ketoglutarate; OAA, oxaloacetate.
    Aspartate Aminotransferase, supplied by Ningbo Purebio Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aspartate aminotransferase/product/Ningbo Purebio Biotechnology
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    aspartate aminotransferase - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

    Image Search Results


    HSV-1 replication is inhibited by reducing flux from glucose toward pyrimidine nucleotide synthesis. ( A ) Schematic diagram of glucose flux to pyrimidine nucleotide biosynthesis. Red lines mark siRNA-targeted reactions catalyzed by pyruvate carboxylase (PC) and aspartate transaminase 2 (GOT2). (OAA: oxaloacetate, AKG: oxoglutarate, Gln: glutamine). ( B ) RNA interference knockdown of pyruvate carboxylase (marked by arrow) in MRC5 cells. Cells were transfected with non-targeting siRNAs (NT) or siRNAs targeting pyruvate carboxylase (PC) and harvested at indicated time points after transfection. Pyruvate carboxylase levels in the cells were detected by western blot using specific antibodies. Beta-actin was employed as a loading control. ( C ) Buildup of 13 C 3 -labeled malate after switching MRC5 cells to uniformly 13 C-labeled glucose medium for 2 hours at 10 hpi of HSV-1 (F) infection. The cells have been transfected with a universal non-targeting siRNA (NT) or an siRNA targeting pyruvate carboxylase (PC) 120 h prior to infection (significance: p = 0.007). Symbols indicate experimental data points ±1 s.d.; n = 3; values are given in arbitrary units. ( D ) Production of infectious HSV-1 (F) and HCMV (AD169) virions in cells transfected with siRNAs against pyruvate carboxylase (PC), aspartate transaminase 2 (GOT2), or a universal negative control (NT). The transfection and infection of MRC5 cells were performed as described in Materials and Methods . Values are expressed relative to non-targeting control (±1 s.d.; n = 3). Conditions resulting in significantly altered virus production (p≤0.05) compared to treatment with the universal negative control are marked with a star.

    Journal: PLoS Pathogens

    Article Title: Divergent Effects of Human Cytomegalovirus and Herpes Simplex Virus-1 on Cellular Metabolism

    doi: 10.1371/journal.ppat.1002124

    Figure Lengend Snippet: HSV-1 replication is inhibited by reducing flux from glucose toward pyrimidine nucleotide synthesis. ( A ) Schematic diagram of glucose flux to pyrimidine nucleotide biosynthesis. Red lines mark siRNA-targeted reactions catalyzed by pyruvate carboxylase (PC) and aspartate transaminase 2 (GOT2). (OAA: oxaloacetate, AKG: oxoglutarate, Gln: glutamine). ( B ) RNA interference knockdown of pyruvate carboxylase (marked by arrow) in MRC5 cells. Cells were transfected with non-targeting siRNAs (NT) or siRNAs targeting pyruvate carboxylase (PC) and harvested at indicated time points after transfection. Pyruvate carboxylase levels in the cells were detected by western blot using specific antibodies. Beta-actin was employed as a loading control. ( C ) Buildup of 13 C 3 -labeled malate after switching MRC5 cells to uniformly 13 C-labeled glucose medium for 2 hours at 10 hpi of HSV-1 (F) infection. The cells have been transfected with a universal non-targeting siRNA (NT) or an siRNA targeting pyruvate carboxylase (PC) 120 h prior to infection (significance: p = 0.007). Symbols indicate experimental data points ±1 s.d.; n = 3; values are given in arbitrary units. ( D ) Production of infectious HSV-1 (F) and HCMV (AD169) virions in cells transfected with siRNAs against pyruvate carboxylase (PC), aspartate transaminase 2 (GOT2), or a universal negative control (NT). The transfection and infection of MRC5 cells were performed as described in Materials and Methods . Values are expressed relative to non-targeting control (±1 s.d.; n = 3). Conditions resulting in significantly altered virus production (p≤0.05) compared to treatment with the universal negative control are marked with a star.

    Article Snippet: siRNA transfection Double stranded siRNA molecules directed against pyruvate carboxylase ( 5′-GACUGUACGCGGCCUUCGATT ), aspartate transaminase 2 ( 5′-CUAUUGAGAGCUUCACACATT ), and a Universal Negative Control (SIC001) were purchased from Sigma.

    Techniques: Transfection, Western Blot, Labeling, Infection, Negative Control

    The mRNA and protein levels of FGF1 in liver were downregulated in the ANIT-induced intrahepatic cholestasis mouse model. Eight-week-old C57BL/6J mice were orally administrated with olive oil (control group) or 75 mg/kg ANIT (cholestasis mouse model) for once and then liver tissues were collected and analyzed after 48 h. (A , B) Evaluation of liver function as determined by serum levels of ALT and AST. (C , D) Serum BA concentrations (C) and hepatic BA pool (D) of each group were measured. (E) The levels of hepatic FGF1 gene expression of each group were examined by real-time polymerase chain reaction (RT-PCR); (F) Representative western blot analysis (up panel) and densitometric quantification (down panel) of FGF1 protein expression in liver tissues from ANIT-induce intrahepatic cholestasis mouse model and control group; data are normalized to GAPDH. Data are presented as mean ± SEM; ****p

    Journal: Frontiers in Pharmacology

    Article Title: Paracrine Fibroblast Growth Factor 1 Functions as Potent Therapeutic Agent for Intrahepatic Cholestasis by Downregulating Synthesis of Bile Acid

    doi: 10.3389/fphar.2019.01515

    Figure Lengend Snippet: The mRNA and protein levels of FGF1 in liver were downregulated in the ANIT-induced intrahepatic cholestasis mouse model. Eight-week-old C57BL/6J mice were orally administrated with olive oil (control group) or 75 mg/kg ANIT (cholestasis mouse model) for once and then liver tissues were collected and analyzed after 48 h. (A , B) Evaluation of liver function as determined by serum levels of ALT and AST. (C , D) Serum BA concentrations (C) and hepatic BA pool (D) of each group were measured. (E) The levels of hepatic FGF1 gene expression of each group were examined by real-time polymerase chain reaction (RT-PCR); (F) Representative western blot analysis (up panel) and densitometric quantification (down panel) of FGF1 protein expression in liver tissues from ANIT-induce intrahepatic cholestasis mouse model and control group; data are normalized to GAPDH. Data are presented as mean ± SEM; ****p

    Article Snippet: Alanine transaminase (ALT) and aspartate transaminase (AST) Assay Kits were purchased from Sigma Aldrich (St. Louis, MO, USA) and performed in accordance with the protocols provided with manufacturers.

    Techniques: Mouse Assay, AST Assay, Expressing, Real-time Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Western Blot

    Effects of hypothermal stress on the mitochondria in the livers of freshwater (FW) and seawater (SW) milkfish. AST, aspartate transaminase; CS, citrate synthase; GDH, glutamate dehydrogenase; GLU, glutamate; α-KG, alpha-ketoglutarate; OAA, oxaloacetate.

    Journal: Scientific Reports

    Article Title: Changes in hypothermal stress-induced hepatic mitochondrial metabolic patterns between fresh water- and seawater-acclimated milkfish, Chanos chanos

    doi: 10.1038/s41598-019-55055-4

    Figure Lengend Snippet: Effects of hypothermal stress on the mitochondria in the livers of freshwater (FW) and seawater (SW) milkfish. AST, aspartate transaminase; CS, citrate synthase; GDH, glutamate dehydrogenase; GLU, glutamate; α-KG, alpha-ketoglutarate; OAA, oxaloacetate.

    Article Snippet: One hundred milligrams of liver tissue homogenate was placed in 500 μL AST assay buffer and all subsequent steps were performed according to the manufacturer’s instructions (K753-100, Biovision).

    Techniques: AST Assay