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    Santa Cruz Biotechnology goat anti asic1
    Endogenous ASIC2 enriches in synaptosomes and co-immunoprecipitates with <t>ASIC1</t>
    Goat Anti Asic1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology asic1
    Acid-sensing ion channel 1 <t>(ASIC1),</t> TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.
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    Alomone Labs asic1
    AT 2 cells contain <t>ACCN2</t> . A : PCR analysis showing expression of ACCN isoforms in cDNA prepared from AT 2 cells. Only ACCN2 <t>(ASIC1)</t> has a visible band. B : PCR reamplification showing expression of ACCN2 variant 2 (ASIC1a, lane 3 ) and lack of expression of ACCN2 variant 1 (ASIC1b, lane 2 ). NCBI, National Center for Biotechnology Information.
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    Santa Cruz Biotechnology dynein ic1 2 cytosolic antibody
    AT 2 cells contain <t>ACCN2</t> . A : PCR analysis showing expression of ACCN isoforms in cDNA prepared from AT 2 cells. Only ACCN2 <t>(ASIC1)</t> has a visible band. B : PCR reamplification showing expression of ACCN2 variant 2 (ASIC1a, lane 3 ) and lack of expression of ACCN2 variant 1 (ASIC1b, lane 2 ). NCBI, National Center for Biotechnology Information.
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    Image Search Results


    Endogenous ASIC2 enriches in synaptosomes and co-immunoprecipitates with ASIC1

    Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience

    Article Title: ASIC2 Subunits Target Acid-Sensing Ion Channels to the Synapse via an Association with PSD-95

    doi: 10.1523/JNEUROSCI.1284-09.2009

    Figure Lengend Snippet: Endogenous ASIC2 enriches in synaptosomes and co-immunoprecipitates with ASIC1

    Article Snippet: Antibody dilutions: anti-HA-HRP 1:750, anti-GFP 1:1000, anti-HA 1:1000, anti-tubulin 1:4000-10000, anti-GluR2/3 1:1000, anti-PSD-95 1:500, rabbit anti-ASIC1 1:3000-5000, goat anti-ASIC1 1:1000, and rabbit anti-ASIC2a 1:50.

    Techniques:

    Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.

    Journal: Frontiers in Cellular Neuroscience

    Article Title: TASK1 and TASK3 Are Coexpressed With ASIC1 in the Ventrolateral Medulla and Contribute to Central Chemoreception in Rats

    doi: 10.3389/fncel.2018.00285

    Figure Lengend Snippet: Acid-sensing ion channel 1 (ASIC1), TASK1 and TASK3 are expressed and colocalized in the VLM neurons of adult rats. (A) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK1-ir (red) and neurofilament-H (blue) in the VLM. (B) Representative high power visual field of the area shown in (A) . (C) Representative confocal photomicrographs showing the colocalization of ASIC1-ir (green), TASK3-ir (red) and neurofilament-H (blue) in the VLM. (D) Representative high power visual field of the area shown in (C) . (A–C) Scale bar = 200 μm; (B,D) scale bar = 20 μm.

    Article Snippet: Immunofluorescence was applied to observe the coexpression of ASIC1 and TASK1 or TASK3.

    Techniques:

    AT 2 cells contain ACCN2 . A : PCR analysis showing expression of ACCN isoforms in cDNA prepared from AT 2 cells. Only ACCN2 (ASIC1) has a visible band. B : PCR reamplification showing expression of ACCN2 variant 2 (ASIC1a, lane 3 ) and lack of expression of ACCN2 variant 1 (ASIC1b, lane 2 ). NCBI, National Center for Biotechnology Information.

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Alveolar nonselective channels are ASIC1a/α-ENaC channels and contribute to AFC

    doi: 10.1152/ajplung.00379.2016

    Figure Lengend Snippet: AT 2 cells contain ACCN2 . A : PCR analysis showing expression of ACCN isoforms in cDNA prepared from AT 2 cells. Only ACCN2 (ASIC1) has a visible band. B : PCR reamplification showing expression of ACCN2 variant 2 (ASIC1a, lane 3 ) and lack of expression of ACCN2 variant 1 (ASIC1b, lane 2 ). NCBI, National Center for Biotechnology Information.

    Article Snippet: To explicitly show this association, we used L2 cell protein lysate to immunoprecipitate (IP) for α-ENaC and immunoblot for ASIC1 and IP for ASIC1 and blot for α-ENaC ( ).

    Techniques: Polymerase Chain Reaction, Expressing, Variant Assay

    KO mice have no detectable ASIC1a. PCR analysis shows expression of ACCN2 in cDNA prepared from wild-type lungs and from rat lung as a positive control. The cDNA prepared from ASIC1 KO lung has no detectable ACCN2 (ASIC1).

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Alveolar nonselective channels are ASIC1a/α-ENaC channels and contribute to AFC

    doi: 10.1152/ajplung.00379.2016

    Figure Lengend Snippet: KO mice have no detectable ASIC1a. PCR analysis shows expression of ACCN2 in cDNA prepared from wild-type lungs and from rat lung as a positive control. The cDNA prepared from ASIC1 KO lung has no detectable ACCN2 (ASIC1).

    Article Snippet: To explicitly show this association, we used L2 cell protein lysate to immunoprecipitate (IP) for α-ENaC and immunoblot for ASIC1 and IP for ASIC1 and blot for α-ENaC ( ).

    Techniques: Mouse Assay, Polymerase Chain Reaction, Expressing, Positive Control

    NSC channel frequency strongly depends on ASIC1a and α-ENaC presence. A : Western blot showing ASIC1 expression in L2 cells treated with scrambled shRNA or ASIC1 silencing vectors. Although the blot is cropped, there are no other bands in the blot. Reduction of ASIC1 protein positively correlates to amount of ASIC1a shRNA used. B , left : percentage patches with NSC channels present under ASIC1 or α-ENaC knockdown conditions. Right : percentage patches with HSC channels under ASIC1 or α-ENaC knockdown conditions. Numbers above bars indicate total number of patches.

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Alveolar nonselective channels are ASIC1a/α-ENaC channels and contribute to AFC

    doi: 10.1152/ajplung.00379.2016

    Figure Lengend Snippet: NSC channel frequency strongly depends on ASIC1a and α-ENaC presence. A : Western blot showing ASIC1 expression in L2 cells treated with scrambled shRNA or ASIC1 silencing vectors. Although the blot is cropped, there are no other bands in the blot. Reduction of ASIC1 protein positively correlates to amount of ASIC1a shRNA used. B , left : percentage patches with NSC channels present under ASIC1 or α-ENaC knockdown conditions. Right : percentage patches with HSC channels under ASIC1 or α-ENaC knockdown conditions. Numbers above bars indicate total number of patches.

    Article Snippet: To explicitly show this association, we used L2 cell protein lysate to immunoprecipitate (IP) for α-ENaC and immunoblot for ASIC1 and IP for ASIC1 and blot for α-ENaC ( ).

    Techniques: Western Blot, Expressing, shRNA

    Interaction of ASIC1a and α-ENaC subunits. A , top : L2 cell protein lysate immunoprecipitated (IP) for α-ENaC and immunoblotted (IB) for ASIC1. Bottom : L2 cell protein lysate immunoprecipitated for ASIC1 and immunoblotted for α-ENaC. B : quantification of mammalian two-hybrid assay between ASIC1a and ENaC subunits. Normalized luciferase luminescence is proportional to binding affinity to ASIC1a. Negative control represents random association, and positive control represents maximum affinity. Fold increase for α-ENaC (6.6 ± 0.73) indicates a high affinity for ASIC1a. Data represent a total n of 15 ( n = 3 for each condition); * P

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Alveolar nonselective channels are ASIC1a/α-ENaC channels and contribute to AFC

    doi: 10.1152/ajplung.00379.2016

    Figure Lengend Snippet: Interaction of ASIC1a and α-ENaC subunits. A , top : L2 cell protein lysate immunoprecipitated (IP) for α-ENaC and immunoblotted (IB) for ASIC1. Bottom : L2 cell protein lysate immunoprecipitated for ASIC1 and immunoblotted for α-ENaC. B : quantification of mammalian two-hybrid assay between ASIC1a and ENaC subunits. Normalized luciferase luminescence is proportional to binding affinity to ASIC1a. Negative control represents random association, and positive control represents maximum affinity. Fold increase for α-ENaC (6.6 ± 0.73) indicates a high affinity for ASIC1a. Data represent a total n of 15 ( n = 3 for each condition); * P

    Article Snippet: To explicitly show this association, we used L2 cell protein lysate to immunoprecipitate (IP) for α-ENaC and immunoblot for ASIC1 and IP for ASIC1 and blot for α-ENaC ( ).

    Techniques: Immunoprecipitation, Two Hybrid Assay, Luciferase, Binding Assay, Negative Control, Positive Control

    ASIC1a KO increases lung water content and reduces alveolar fluid clearance. A : lung wet wt-to-dry wt ratios. Higher wet wt-to-dry wt ratio indicates increased lung water content and decreased alveolar fluid clearance. The difference in the two groups is significant. Data represent n = 8 for each treatment group. B : Evans blue dye assay showed that alveolar fluid clearance was significantly reduced in ASIC1a KO mice compared with wild-type mice. Amiloride blocked about half of AFC in wild-type mice, but there is little residual AFC after amiloride in ASIC1 KO mice. Data represent a total n = 3 mice for each treatment group; n.s., not significant. C : bronchalveolar lavage (BAL) fluid protein from wild-type and ASIC1 KO mice. There is no significant difference in BAL protein between the two groups ( n = 4 mice for each treatment group; P = 0.424).

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Alveolar nonselective channels are ASIC1a/α-ENaC channels and contribute to AFC

    doi: 10.1152/ajplung.00379.2016

    Figure Lengend Snippet: ASIC1a KO increases lung water content and reduces alveolar fluid clearance. A : lung wet wt-to-dry wt ratios. Higher wet wt-to-dry wt ratio indicates increased lung water content and decreased alveolar fluid clearance. The difference in the two groups is significant. Data represent n = 8 for each treatment group. B : Evans blue dye assay showed that alveolar fluid clearance was significantly reduced in ASIC1a KO mice compared with wild-type mice. Amiloride blocked about half of AFC in wild-type mice, but there is little residual AFC after amiloride in ASIC1 KO mice. Data represent a total n = 3 mice for each treatment group; n.s., not significant. C : bronchalveolar lavage (BAL) fluid protein from wild-type and ASIC1 KO mice. There is no significant difference in BAL protein between the two groups ( n = 4 mice for each treatment group; P = 0.424).

    Article Snippet: To explicitly show this association, we used L2 cell protein lysate to immunoprecipitate (IP) for α-ENaC and immunoblot for ASIC1 and IP for ASIC1 and blot for α-ENaC ( ).

    Techniques: Mouse Assay

    NSC channels are not observable in single-channel patches on lung slices from ASIC1 KO mice. Single-channel recordings were measured from AT 2 cells in lung slices. A : single-channel currents ( right ) and distribution of current amplitudes ( left ) in a patch on an AT 2 cell from a wild-type lung slice, which has both NSC and HSC channels (sometimes overlapping). B : single-channel currents ( right ) and distribution of current amplitudes ( left ) in a patch on an AT 2 cell from an ASIC1 KO lung slice, which has only HSC channels.

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Alveolar nonselective channels are ASIC1a/α-ENaC channels and contribute to AFC

    doi: 10.1152/ajplung.00379.2016

    Figure Lengend Snippet: NSC channels are not observable in single-channel patches on lung slices from ASIC1 KO mice. Single-channel recordings were measured from AT 2 cells in lung slices. A : single-channel currents ( right ) and distribution of current amplitudes ( left ) in a patch on an AT 2 cell from a wild-type lung slice, which has both NSC and HSC channels (sometimes overlapping). B : single-channel currents ( right ) and distribution of current amplitudes ( left ) in a patch on an AT 2 cell from an ASIC1 KO lung slice, which has only HSC channels.

    Article Snippet: To explicitly show this association, we used L2 cell protein lysate to immunoprecipitate (IP) for α-ENaC and immunoblot for ASIC1 and IP for ASIC1 and blot for α-ENaC ( ).

    Techniques: Mouse Assay

    NSC channels are sensitive to ASIC1-modifying toxins. A : NSC channels were activated by venom of the Texas coral snake ( Micrurus tener tener ). B : psalmotoxin-1 isolated from the venom of the spider, Psalmopoeus cambridgei (Trinidad chevron tarantula), is a potent and selective acid-sensing ion channel 1a (ASIC1a) blocker (IC 50 ). When applied to AT 2 cells in primary culture, it uniformly decreased NSC open probability. Neither toxin had any effect on HSC channels. * P

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Alveolar nonselective channels are ASIC1a/α-ENaC channels and contribute to AFC

    doi: 10.1152/ajplung.00379.2016

    Figure Lengend Snippet: NSC channels are sensitive to ASIC1-modifying toxins. A : NSC channels were activated by venom of the Texas coral snake ( Micrurus tener tener ). B : psalmotoxin-1 isolated from the venom of the spider, Psalmopoeus cambridgei (Trinidad chevron tarantula), is a potent and selective acid-sensing ion channel 1a (ASIC1a) blocker (IC 50 ). When applied to AT 2 cells in primary culture, it uniformly decreased NSC open probability. Neither toxin had any effect on HSC channels. * P

    Article Snippet: To explicitly show this association, we used L2 cell protein lysate to immunoprecipitate (IP) for α-ENaC and immunoblot for ASIC1 and IP for ASIC1 and blot for α-ENaC ( ).

    Techniques: Isolation