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  • 99
    ATCC f12 medium
    F12 Medium, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 161 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher lipofectamine 2000
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    TaKaRa clontech cdna
    Clontech Cdna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa sybr premi e taq
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    Roche fugene 6 transfection reagent
    Fugene 6 Transfection Reagent, supplied by Roche, used in various techniques. Bioz Stars score: 94/100, based on 20149 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher pcdna3 constructors
    Pcdna3 Constructors, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega transfast transfection reagent
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    Carl Roth GmbH rotifect
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    World Precision Instruments pv820 pneumatic picopump
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    Roche green fluorescent protein gfp
    Green Fluorescent Protein Gfp, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa express c1
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    Thermo Fisher expression vector pcdna3 1
    Expression Vector Pcdna3 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3561 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher amplitaq dna polymerase
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    Qiagen sybrgreen reaction mix
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    Thermo Fisher taqman gene expression assay
    Taqman Gene Expression Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 12710 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher topo ta cloning kit
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    Thermo Fisher superscript ii reverse transcriptase
    Superscript Ii Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 90426 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen d54 mg cells
    Inhibition of ASIC-1 by PcTX-1 reduces migration and proliferation of <t>D54-MG</t> glioma cells. A , representative microscopic images of transwell filters, showing migration of D54-MG cells following a 24-h exposure to PcTX-1, control peptide ( Cont_Pep ), or benzamil ( Benz ). Drugs were present on both sides of the filter throughout the 5-h migration period. In each experiment, the number of cells on the underside of the filter was counted from five randomly selected fields of each image ( n ≥3). B, bar graph showing the average number of migrated cells per field under each condition (normalized to control). C , scratch/wound assay. Phase contrast image of D54-MG cells untreated or treated with PcTX-1, control peptide, or benzamil at 0 and 24 h after scarring. D , summary bar graph showing results expressed as % wound area covered at 24 h, normalized to control conditions ( n ≥4).
    D54 Mg Cells, supplied by Qiagen, used in various techniques. Bioz Stars score: 88/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher gfp
    Inhibition of ASIC-1 by PcTX-1 reduces migration and proliferation of <t>D54-MG</t> glioma cells. A , representative microscopic images of transwell filters, showing migration of D54-MG cells following a 24-h exposure to PcTX-1, control peptide ( Cont_Pep ), or benzamil ( Benz ). Drugs were present on both sides of the filter throughout the 5-h migration period. In each experiment, the number of cells on the underside of the filter was counted from five randomly selected fields of each image ( n ≥3). B, bar graph showing the average number of migrated cells per field under each condition (normalized to control). C , scratch/wound assay. Phase contrast image of D54-MG cells untreated or treated with PcTX-1, control peptide, or benzamil at 0 and 24 h after scarring. D , summary bar graph showing results expressed as % wound area covered at 24 h, normalized to control conditions ( n ≥4).
    Gfp, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 24758 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa pegfp n1 clontech vector
    Inhibition of ASIC-1 by PcTX-1 reduces migration and proliferation of <t>D54-MG</t> glioma cells. A , representative microscopic images of transwell filters, showing migration of D54-MG cells following a 24-h exposure to PcTX-1, control peptide ( Cont_Pep ), or benzamil ( Benz ). Drugs were present on both sides of the filter throughout the 5-h migration period. In each experiment, the number of cells on the underside of the filter was counted from five randomly selected fields of each image ( n ≥3). B, bar graph showing the average number of migrated cells per field under each condition (normalized to control). C , scratch/wound assay. Phase contrast image of D54-MG cells untreated or treated with PcTX-1, control peptide, or benzamil at 0 and 24 h after scarring. D , summary bar graph showing results expressed as % wound area covered at 24 h, normalized to control conditions ( n ≥4).
    Pegfp N1 Clontech Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 89/100, based on 161 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Stratagene quick change kit
    Inhibition of ASIC-1 by PcTX-1 reduces migration and proliferation of <t>D54-MG</t> glioma cells. A , representative microscopic images of transwell filters, showing migration of D54-MG cells following a 24-h exposure to PcTX-1, control peptide ( Cont_Pep ), or benzamil ( Benz ). Drugs were present on both sides of the filter throughout the 5-h migration period. In each experiment, the number of cells on the underside of the filter was counted from five randomly selected fields of each image ( n ≥3). B, bar graph showing the average number of migrated cells per field under each condition (normalized to control). C , scratch/wound assay. Phase contrast image of D54-MG cells untreated or treated with PcTX-1, control peptide, or benzamil at 0 and 24 h after scarring. D , summary bar graph showing results expressed as % wound area covered at 24 h, normalized to control conditions ( n ≥4).
    Quick Change Kit, supplied by Stratagene, used in various techniques. Bioz Stars score: 93/100, based on 1660 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Thermo Fisher pcdna6 myc
    Inhibition of ASIC-1 by PcTX-1 reduces migration and proliferation of <t>D54-MG</t> glioma cells. A , representative microscopic images of transwell filters, showing migration of D54-MG cells following a 24-h exposure to PcTX-1, control peptide ( Cont_Pep ), or benzamil ( Benz ). Drugs were present on both sides of the filter throughout the 5-h migration period. In each experiment, the number of cells on the underside of the filter was counted from five randomly selected fields of each image ( n ≥3). B, bar graph showing the average number of migrated cells per field under each condition (normalized to control). C , scratch/wound assay. Phase contrast image of D54-MG cells untreated or treated with PcTX-1, control peptide, or benzamil at 0 and 24 h after scarring. D , summary bar graph showing results expressed as % wound area covered at 24 h, normalized to control conditions ( n ≥4).
    Pcdna6 Myc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 151 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Inhibition of ASIC-1 by PcTX-1 reduces migration and proliferation of D54-MG glioma cells. A , representative microscopic images of transwell filters, showing migration of D54-MG cells following a 24-h exposure to PcTX-1, control peptide ( Cont_Pep ), or benzamil ( Benz ). Drugs were present on both sides of the filter throughout the 5-h migration period. In each experiment, the number of cells on the underside of the filter was counted from five randomly selected fields of each image ( n ≥3). B, bar graph showing the average number of migrated cells per field under each condition (normalized to control). C , scratch/wound assay. Phase contrast image of D54-MG cells untreated or treated with PcTX-1, control peptide, or benzamil at 0 and 24 h after scarring. D , summary bar graph showing results expressed as % wound area covered at 24 h, normalized to control conditions ( n ≥4).

    Journal: The Journal of Biological Chemistry

    Article Title: Glioma-specific Cation Conductance Regulates Migration and Cell Cycle Progression *

    doi: 10.1074/jbc.M111.311688

    Figure Lengend Snippet: Inhibition of ASIC-1 by PcTX-1 reduces migration and proliferation of D54-MG glioma cells. A , representative microscopic images of transwell filters, showing migration of D54-MG cells following a 24-h exposure to PcTX-1, control peptide ( Cont_Pep ), or benzamil ( Benz ). Drugs were present on both sides of the filter throughout the 5-h migration period. In each experiment, the number of cells on the underside of the filter was counted from five randomly selected fields of each image ( n ≥3). B, bar graph showing the average number of migrated cells per field under each condition (normalized to control). C , scratch/wound assay. Phase contrast image of D54-MG cells untreated or treated with PcTX-1, control peptide, or benzamil at 0 and 24 h after scarring. D , summary bar graph showing results expressed as % wound area covered at 24 h, normalized to control conditions ( n ≥4).

    Article Snippet: We analyzed whole-cell lysates obtained from both wild type D54-MG (D54MG-WT) cells and D54-MG cells stably transfected with the DN-eGFP-ASIC1 cDNA (D54MG-A1DN) for ASIC-1 protein expression.

    Techniques: Inhibition, Migration, Scratch Wound Assay Assay

    Knocking down δENaC has no effect CKI expression. A , lysates from untransfected wild type D54-MG and D54MG-δENaC-DN cells were separated by SDS-PAGE and immunoblotting ( IB ). Each bar represents the normalized density of both bands compared with untransfected D54-MG cells, n ≥3. B , no difference in expression of p21 Cip1 and p27 kip1 proteins was detected between wild type D54-MG and D54MG-δENaC-DN cells. Wild type cells treated with benzamil were used as a positive control ( n ≥6).

    Journal: The Journal of Biological Chemistry

    Article Title: Glioma-specific Cation Conductance Regulates Migration and Cell Cycle Progression *

    doi: 10.1074/jbc.M111.311688

    Figure Lengend Snippet: Knocking down δENaC has no effect CKI expression. A , lysates from untransfected wild type D54-MG and D54MG-δENaC-DN cells were separated by SDS-PAGE and immunoblotting ( IB ). Each bar represents the normalized density of both bands compared with untransfected D54-MG cells, n ≥3. B , no difference in expression of p21 Cip1 and p27 kip1 proteins was detected between wild type D54-MG and D54MG-δENaC-DN cells. Wild type cells treated with benzamil were used as a positive control ( n ≥6).

    Article Snippet: We analyzed whole-cell lysates obtained from both wild type D54-MG (D54MG-WT) cells and D54-MG cells stably transfected with the DN-eGFP-ASIC1 cDNA (D54MG-A1DN) for ASIC-1 protein expression.

    Techniques: Expressing, SDS Page, Positive Control

    Effect of inhibition of ASIC-1 on cell cycle progression and whole-cell current of D54-MG glioma cells. A , flow cytometric analysis of D54-MG cells treated with PcTX-1, control peptide ( Con_Pep ), and benzamil ( Benz ) for 24 h. DNA content is shown as 2 n and 4 n in the x axis where 2 n = cells in G 0 /G 1 phase and 4 n = cells in G 2 /M phase. The bar graph shows the percentage of cells in each cell cycle phase for the different experimental conditions, n ≥4. B , expression of p21 Cip1 and p27 Kip1 proteins in D54-MG cells treated with PcTX-1, control peptide, or benzamil for 24 h. β-Actin was used as a loading control. Accompanying bar graph shows the normalized density as compared with 2% FBS control n ≥6. IB , immunoblot. C, bar graph showing average amiloride-sensitive currents at −80 mV for D54-MG cells pretreated with PcTX-1, control peptide, or benzamil for 24 h. Currents were recorded in the continued presence of drug in the bath solution n ≥6. D , summary I/V curves showing basal current and that following addition of amiloride to the bath solution.

    Journal: The Journal of Biological Chemistry

    Article Title: Glioma-specific Cation Conductance Regulates Migration and Cell Cycle Progression *

    doi: 10.1074/jbc.M111.311688

    Figure Lengend Snippet: Effect of inhibition of ASIC-1 on cell cycle progression and whole-cell current of D54-MG glioma cells. A , flow cytometric analysis of D54-MG cells treated with PcTX-1, control peptide ( Con_Pep ), and benzamil ( Benz ) for 24 h. DNA content is shown as 2 n and 4 n in the x axis where 2 n = cells in G 0 /G 1 phase and 4 n = cells in G 2 /M phase. The bar graph shows the percentage of cells in each cell cycle phase for the different experimental conditions, n ≥4. B , expression of p21 Cip1 and p27 Kip1 proteins in D54-MG cells treated with PcTX-1, control peptide, or benzamil for 24 h. β-Actin was used as a loading control. Accompanying bar graph shows the normalized density as compared with 2% FBS control n ≥6. IB , immunoblot. C, bar graph showing average amiloride-sensitive currents at −80 mV for D54-MG cells pretreated with PcTX-1, control peptide, or benzamil for 24 h. Currents were recorded in the continued presence of drug in the bath solution n ≥6. D , summary I/V curves showing basal current and that following addition of amiloride to the bath solution.

    Article Snippet: We analyzed whole-cell lysates obtained from both wild type D54-MG (D54MG-WT) cells and D54-MG cells stably transfected with the DN-eGFP-ASIC1 cDNA (D54MG-A1DN) for ASIC-1 protein expression.

    Techniques: Inhibition, Flow Cytometry, Expressing

    Inhibition of ERK1/2 phosphorylation. A , immunoblot ( IB ) analysis of lysates from D54-MG cells treated with PcTX-1, control peptide ( Con_Pep ), or benzamil ( Benz ) for 24 h. The blots were probed for phospho-ERK1/2 and then stripped and reprobed for total ERK1/2, n ≥3. Con , control. B , immunoblot analysis of lysates from D54MG-WT, D54MG-A1DN cells, and D54MG-WT + benzamil, n ≥6. C , knockdown of δENaC did not affect the phosphorylation of ERK1/2 compared with wild type D54MG cells, n ≥4. In each case, the bar graph illustrates the normalized density compared with untreated cells exposed to 2% FBS. D , low [Na + ] also down-regulated phosphorylation of ERK1/2 in D54-MG glioma cells following 24 h of incubation. Each bar represents the normalized density compared with cells normal Kreb's buffer ( n ≥4).

    Journal: The Journal of Biological Chemistry

    Article Title: Glioma-specific Cation Conductance Regulates Migration and Cell Cycle Progression *

    doi: 10.1074/jbc.M111.311688

    Figure Lengend Snippet: Inhibition of ERK1/2 phosphorylation. A , immunoblot ( IB ) analysis of lysates from D54-MG cells treated with PcTX-1, control peptide ( Con_Pep ), or benzamil ( Benz ) for 24 h. The blots were probed for phospho-ERK1/2 and then stripped and reprobed for total ERK1/2, n ≥3. Con , control. B , immunoblot analysis of lysates from D54MG-WT, D54MG-A1DN cells, and D54MG-WT + benzamil, n ≥6. C , knockdown of δENaC did not affect the phosphorylation of ERK1/2 compared with wild type D54MG cells, n ≥4. In each case, the bar graph illustrates the normalized density compared with untreated cells exposed to 2% FBS. D , low [Na + ] also down-regulated phosphorylation of ERK1/2 in D54-MG glioma cells following 24 h of incubation. Each bar represents the normalized density compared with cells normal Kreb's buffer ( n ≥4).

    Article Snippet: We analyzed whole-cell lysates obtained from both wild type D54-MG (D54MG-WT) cells and D54-MG cells stably transfected with the DN-eGFP-ASIC1 cDNA (D54MG-A1DN) for ASIC-1 protein expression.

    Techniques: Inhibition, Incubation

    Knockdown of ASIC1 expression inhibits amiloride-sensitive current in D54-MG glioma cells. A , lysates from untransfected wild type D54-MG ( D54MG-WT ) and D54-MG cells stably transfected with DN-eGFP-ASIC1 cDNA ( D54MG-A1DN ) were analyzed. The expression of ASIC-1 was significantly decreased in D54MG-A1DN cells compared with wild type cells, n ≥6. IB , immunoblot. B , average amiloride-sensitive currents at −80 mV, n ≥6. C, I/V curves show significant attenuation of amiloride-sensitive whole-cell current after knocking down ASIC1.

    Journal: The Journal of Biological Chemistry

    Article Title: Glioma-specific Cation Conductance Regulates Migration and Cell Cycle Progression *

    doi: 10.1074/jbc.M111.311688

    Figure Lengend Snippet: Knockdown of ASIC1 expression inhibits amiloride-sensitive current in D54-MG glioma cells. A , lysates from untransfected wild type D54-MG ( D54MG-WT ) and D54-MG cells stably transfected with DN-eGFP-ASIC1 cDNA ( D54MG-A1DN ) were analyzed. The expression of ASIC-1 was significantly decreased in D54MG-A1DN cells compared with wild type cells, n ≥6. IB , immunoblot. B , average amiloride-sensitive currents at −80 mV, n ≥6. C, I/V curves show significant attenuation of amiloride-sensitive whole-cell current after knocking down ASIC1.

    Article Snippet: We analyzed whole-cell lysates obtained from both wild type D54-MG (D54MG-WT) cells and D54-MG cells stably transfected with the DN-eGFP-ASIC1 cDNA (D54MG-A1DN) for ASIC-1 protein expression.

    Techniques: Expressing, Stable Transfection, Transfection