arabinoxylan Search Results


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  • 98
    Millipore arabinoxylan
    Arabinoxylan, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    BioActor arabinoxylan
    Effects of the wheat-derived <t>arabinoxylan</t> (AX) on colonic paracellular permeability in biopsies mounted in Ussing chambers. Stimulation with Compound (C) 48/80 (10 ng/ml) resulted in a significantly higher paracellular permeability compared to vehicle ( a , b ). Co-stimulation with AX (0.1 mg/ml) showed a significant decrease of C48/80-induced hyperpermeability on paracellular passage in only the healthy controls ( b ). Stimulation with AX only had no significant effect on neither paracellular nor transcellular permeability compared to vehicle. *p
    Arabinoxylan, supplied by BioActor, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Megazyme arabinoxylan
    Percentages of <t>arabinoxylan</t> conversion for nine surface-engineered yeast strains were compared after 17 h, 48 h, and 72 h. The differences between CipA1-based minihemicellulosomes and the unifunctional CipA3-based minihemicellulosomes reflect enzyme-enzyme synergy. The differences between the CipA3-based and the corresponding CipA1-based minihemicellulosomes reflect enzyme proximity synergy.
    Arabinoxylan, supplied by Megazyme, used in various techniques. Bioz Stars score: 89/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Megazyme insoluble arabinoxylan
    Percentages of <t>arabinoxylan</t> conversion for nine surface-engineered yeast strains were compared after 17 h, 48 h, and 72 h. The differences between CipA1-based minihemicellulosomes and the unifunctional CipA3-based minihemicellulosomes reflect enzyme-enzyme synergy. The differences between the CipA3-based and the corresponding CipA1-based minihemicellulosomes reflect enzyme proximity synergy.
    Insoluble Arabinoxylan, supplied by Megazyme, used in various techniques. Bioz Stars score: 85/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Megazyme azcl arabinoxylan
    Expression of r Tr XYNII in Y. lipolytica . a Screening of Y. lipolytica transformants on agar plates containing 0.2% w/v <t>AZCL-arabinoxylan:</t> lane 1, Y. lipolytica -control; lane 2 to 3, Y. lipolytica expressing r Tr XYNII and rh Tr XYNII respectively; L4, YLC8; b r Tr XYNII production on YTD medium versus time; c SDS-PAGE analysis of the culture supernatant of Y. lipolytica control strain expressing empty plasmid and the recombinant strain expressing rh Tr XYNII; d SDS-PAGE analysis of the rh Tr XYNII after purification, protein marker (lane 1) and rh Tr XYNII (lane 2)
    Azcl Arabinoxylan, supplied by Megazyme, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Megazyme wheat arabinoxylan
    Hydrolysis of water-soluble wheat <t>arabinoxylan</t> by purified rPcAxe. 1, Substrate incubated without enzyme for 24 h (control); 2, hydrolysate treated with rPcAxe for 24 h; 3, arabinose standard; 4, xylose standard. Hydrolysis reactions were performed at 37 °C in buffer consisting of mixtures of 0.1 M citric acid and 0.2 M sodium hydrogen phosphate (pH 7.0) for 24 h with 1% (w/v) water-soluble wheat arabinoxylan loading. The amount of arabinose and xylose released was determined using HPLC. Values are the means and standard deviations of triplicate experiments
    Wheat Arabinoxylan, supplied by Megazyme, used in various techniques. Bioz Stars score: 91/100, based on 304 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Novozymes arabinoxylan
    Hydrolysis of water-soluble wheat <t>arabinoxylan</t> by purified rPcAxe. 1, Substrate incubated without enzyme for 24 h (control); 2, hydrolysate treated with rPcAxe for 24 h; 3, arabinose standard; 4, xylose standard. Hydrolysis reactions were performed at 37 °C in buffer consisting of mixtures of 0.1 M citric acid and 0.2 M sodium hydrogen phosphate (pH 7.0) for 24 h with 1% (w/v) water-soluble wheat arabinoxylan loading. The amount of arabinose and xylose released was determined using HPLC. Values are the means and standard deviations of triplicate experiments
    Arabinoxylan, supplied by Novozymes, used in various techniques. Bioz Stars score: 89/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Megazyme soluble wheat arabinoxylan
    Schematic diagram of the domain architecture of XynB and its mutants. The specific activities of XynB and its mutants toward 30 mg/ml <t>arabinoxylan</t> or beechwood xylan were determined at 40°C and pH 7.0. The data shown in the graph are from triplicate experiments (means ± standard deviations [SD]). ND indicates that enzyme activity was not detectable.
    Soluble Wheat Arabinoxylan, supplied by Megazyme, used in various techniques. Bioz Stars score: 89/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Millipore wheat arabinoxylan
    Schematic diagram of the domain architecture of XynB and its mutants. The specific activities of XynB and its mutants toward 30 mg/ml <t>arabinoxylan</t> or beechwood xylan were determined at 40°C and pH 7.0. The data shown in the graph are from triplicate experiments (means ± standard deviations [SD]). ND indicates that enzyme activity was not detectable.
    Wheat Arabinoxylan, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Megazyme insoluble wheat arabinoxylan
    Release of ferulic acid from (A) 1% wheat <t>arabinoxylan</t> ( WAX ) and (B) 1% sugar beet pectin ( SBP ) using AtFaeD with and without pre‐treatment with commercial xylanase and endopolygalacturonase, respectively. WAX was also co‐incubated with AtFaeD and commercial xylanase. Mean values and standard deviations from three replicate experiments are presented.
    Insoluble Wheat Arabinoxylan, supplied by Megazyme, used in various techniques. Bioz Stars score: 89/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Megazyme arabinoxylan p waxyrs
    Release of ferulic acid from (A) 1% wheat <t>arabinoxylan</t> ( WAX ) and (B) 1% sugar beet pectin ( SBP ) using AtFaeD with and without pre‐treatment with commercial xylanase and endopolygalacturonase, respectively. WAX was also co‐incubated with AtFaeD and commercial xylanase. Mean values and standard deviations from three replicate experiments are presented.
    Arabinoxylan P Waxyrs, supplied by Megazyme, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Megazyme low viscosity wheat arabinoxylan
    HPAEC analysis of end products released by endo-acting P. anserina hemicellulases. (A) Ivory nut mannan hydrolysis by Pa Man5A and Pa Man26A: mannose (M1), mannobiose (M2), and mannotriose (M3). (B) Wheat <t>arabinoxylan</t> hydrolysis by Pa Xyn11A: xylose (X1),
    Low Viscosity Wheat Arabinoxylan, supplied by Megazyme, used in various techniques. Bioz Stars score: 88/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Megazyme water soluble wheat arabinoxylan
    HPAEC analysis of end products released by endo-acting P. anserina hemicellulases. (A) Ivory nut mannan hydrolysis by Pa Man5A and Pa Man26A: mannose (M1), mannobiose (M2), and mannotriose (M3). (B) Wheat <t>arabinoxylan</t> hydrolysis by Pa Xyn11A: xylose (X1),
    Water Soluble Wheat Arabinoxylan, supplied by Megazyme, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Megazyme azo functionalized arabinoxylans
    HPAEC analysis of end products released by endo-acting P. anserina hemicellulases. (A) Ivory nut mannan hydrolysis by Pa Man5A and Pa Man26A: mannose (M1), mannobiose (M2), and mannotriose (M3). (B) Wheat <t>arabinoxylan</t> hydrolysis by Pa Xyn11A: xylose (X1),
    Azo Functionalized Arabinoxylans, supplied by Megazyme, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Megazyme arabinoxylan p raxy
    HPAEC analysis of end products released by endo-acting P. anserina hemicellulases. (A) Ivory nut mannan hydrolysis by Pa Man5A and Pa Man26A: mannose (M1), mannobiose (M2), and mannotriose (M3). (B) Wheat <t>arabinoxylan</t> hydrolysis by Pa Xyn11A: xylose (X1),
    Arabinoxylan P Raxy, supplied by Megazyme, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore soluble insoluble wheat arabinoxylan
    HPAEC analysis of end products released by endo-acting P. anserina hemicellulases. (A) Ivory nut mannan hydrolysis by Pa Man5A and Pa Man26A: mannose (M1), mannobiose (M2), and mannotriose (M3). (B) Wheat <t>arabinoxylan</t> hydrolysis by Pa Xyn11A: xylose (X1),
    Soluble Insoluble Wheat Arabinoxylan, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Effects of the wheat-derived arabinoxylan (AX) on colonic paracellular permeability in biopsies mounted in Ussing chambers. Stimulation with Compound (C) 48/80 (10 ng/ml) resulted in a significantly higher paracellular permeability compared to vehicle ( a , b ). Co-stimulation with AX (0.1 mg/ml) showed a significant decrease of C48/80-induced hyperpermeability on paracellular passage in only the healthy controls ( b ). Stimulation with AX only had no significant effect on neither paracellular nor transcellular permeability compared to vehicle. *p

    Journal: Scientific Reports

    Article Title: Differential effects of dietary fibres on colonic barrier function in elderly individuals with gastrointestinal symptoms

    doi: 10.1038/s41598-018-31492-5

    Figure Lengend Snippet: Effects of the wheat-derived arabinoxylan (AX) on colonic paracellular permeability in biopsies mounted in Ussing chambers. Stimulation with Compound (C) 48/80 (10 ng/ml) resulted in a significantly higher paracellular permeability compared to vehicle ( a , b ). Co-stimulation with AX (0.1 mg/ml) showed a significant decrease of C48/80-induced hyperpermeability on paracellular passage in only the healthy controls ( b ). Stimulation with AX only had no significant effect on neither paracellular nor transcellular permeability compared to vehicle. *p

    Article Snippet: Soluble β-1,3/1,6-glucan (0.5 mg/ml) from Baker’s yeast (Biothera, Eagan, MN, USA) or arabinoxylan (0.1 mg/ml) (Bioactor BV, the Netherlands and Nofima, Norway) was added to the mucosal side of designated chambers, and after 20 min, C48/80 (10 ng/ml) was added to the serosal side.

    Techniques: Derivative Assay, Permeability

    Effects of the wheat-derived arabinoxylan (AX) on colonic transcellular permeability in biopsies mounted in Ussing chambers. Stimulation with Compound (C) 48/80 (10 ng/ml) resulted in a significantly higher transcellular passage of horseradish peroxidase (HRP) compared to vehicle ( a , b ). Co-stimulation with AX (0.1 mg/ml) showed a significant decrease of C48/80-induced transcellular permeability in both elderly with GI symptoms and healthy controls ( b ). Stimulation with AX only had no significant effect on neither paracellular nor transcellular permeability compared to vehicle. *p

    Journal: Scientific Reports

    Article Title: Differential effects of dietary fibres on colonic barrier function in elderly individuals with gastrointestinal symptoms

    doi: 10.1038/s41598-018-31492-5

    Figure Lengend Snippet: Effects of the wheat-derived arabinoxylan (AX) on colonic transcellular permeability in biopsies mounted in Ussing chambers. Stimulation with Compound (C) 48/80 (10 ng/ml) resulted in a significantly higher transcellular passage of horseradish peroxidase (HRP) compared to vehicle ( a , b ). Co-stimulation with AX (0.1 mg/ml) showed a significant decrease of C48/80-induced transcellular permeability in both elderly with GI symptoms and healthy controls ( b ). Stimulation with AX only had no significant effect on neither paracellular nor transcellular permeability compared to vehicle. *p

    Article Snippet: Soluble β-1,3/1,6-glucan (0.5 mg/ml) from Baker’s yeast (Biothera, Eagan, MN, USA) or arabinoxylan (0.1 mg/ml) (Bioactor BV, the Netherlands and Nofima, Norway) was added to the mucosal side of designated chambers, and after 20 min, C48/80 (10 ng/ml) was added to the serosal side.

    Techniques: Derivative Assay, Permeability

    Percentages of arabinoxylan conversion for nine surface-engineered yeast strains were compared after 17 h, 48 h, and 72 h. The differences between CipA1-based minihemicellulosomes and the unifunctional CipA3-based minihemicellulosomes reflect enzyme-enzyme synergy. The differences between the CipA3-based and the corresponding CipA1-based minihemicellulosomes reflect enzyme proximity synergy.

    Journal: Applied and Environmental Microbiology

    Article Title: Direct Conversion of Xylan to Ethanol by Recombinant Saccharomyces cerevisiae Strains Displaying an Engineered Minihemicellulosome

    doi: 10.1128/AEM.07679-11

    Figure Lengend Snippet: Percentages of arabinoxylan conversion for nine surface-engineered yeast strains were compared after 17 h, 48 h, and 72 h. The differences between CipA1-based minihemicellulosomes and the unifunctional CipA3-based minihemicellulosomes reflect enzyme-enzyme synergy. The differences between the CipA3-based and the corresponding CipA1-based minihemicellulosomes reflect enzyme proximity synergy.

    Article Snippet: The substrates, arabinoxylan (catalog number P-WAXYM) and xylooligosaccharides (xylobiose, xylotriose, and xylotetraose), were purchased from Megazyme (Bray Co., Wicklow, Ireland).

    Techniques:

    Functional analysis of surface-displayed CipA3-based minihemicellulosomes. (A) Hydrolysis of arabinoxylan from CipA3-based uni-, bi-, and trifunctional minihemicellulosomes. (B) Hydrolysis of birchwood xylan from CipA3-based uni-, bi-, and trifunctional minihemicellulosomes. The hydrolysis reactions were performed with 50 mM potassium acetate buffer (pH 5.0) with 1% (wt/vol) arabinoxylan or 0.1% (wt/vol) birchwood xylan to an OD 600 of ∼10. The experiments were carried out with serum bottles at 30°C at 200 rpm. All the experiments were done in triplicate, and the averages and standard deviations are plotted.

    Journal: Applied and Environmental Microbiology

    Article Title: Direct Conversion of Xylan to Ethanol by Recombinant Saccharomyces cerevisiae Strains Displaying an Engineered Minihemicellulosome

    doi: 10.1128/AEM.07679-11

    Figure Lengend Snippet: Functional analysis of surface-displayed CipA3-based minihemicellulosomes. (A) Hydrolysis of arabinoxylan from CipA3-based uni-, bi-, and trifunctional minihemicellulosomes. (B) Hydrolysis of birchwood xylan from CipA3-based uni-, bi-, and trifunctional minihemicellulosomes. The hydrolysis reactions were performed with 50 mM potassium acetate buffer (pH 5.0) with 1% (wt/vol) arabinoxylan or 0.1% (wt/vol) birchwood xylan to an OD 600 of ∼10. The experiments were carried out with serum bottles at 30°C at 200 rpm. All the experiments were done in triplicate, and the averages and standard deviations are plotted.

    Article Snippet: The substrates, arabinoxylan (catalog number P-WAXYM) and xylooligosaccharides (xylobiose, xylotriose, and xylotetraose), were purchased from Megazyme (Bray Co., Wicklow, Ireland).

    Techniques: Functional Assay

    Adsorption of rCBM to soluble xylans of different origins. Affinities of rCBM (lane 2) for rye arabinoxylan (B), birchwood xylan (C), and oat spelt xylan (D) were analyzed by native affinity gel electrophoresis. Lane 1 contains BSA as a control protein. (A) A gel without a polysaccharide served as a reference.

    Journal: Applied and Environmental Microbiology

    Article Title: Characterization of Paenibacillus curdlanolyticus B-6 Xyn10D, a Xylanase That Contains a Family 3 Carbohydrate-Binding Module ▿ B-6 Xyn10D, a Xylanase That Contains a Family 3 Carbohydrate-Binding Module ▿ †

    doi: 10.1128/AEM.00226-11

    Figure Lengend Snippet: Adsorption of rCBM to soluble xylans of different origins. Affinities of rCBM (lane 2) for rye arabinoxylan (B), birchwood xylan (C), and oat spelt xylan (D) were analyzed by native affinity gel electrophoresis. Lane 1 contains BSA as a control protein. (A) A gel without a polysaccharide served as a reference.

    Article Snippet: They were most active toward rye arabinoxylan.

    Techniques: Adsorption, Nucleic Acid Electrophoresis

    Adsorption of rCBM to Avicel and insoluble xylan preparations. rCBM was incubated with Avicel (A), an insoluble fraction of oat spelt xylan (B), and insoluble wheat arabinoxylan (C). After centrifugation, proteins in the supernatant (lane 1), wash (lane 2), and precipitate (lane 3) fractions were analyzed by SDS-PAGE. In panel D, BSA as a control protein was incubated with an insoluble fraction of oat spelt xylan, and each fraction was subjected to SDS-PAGE. In panels A to C, 15% polyacrylamide gels were used. In panel D, a 7.5% polyacrylamide gel was used.

    Journal: Applied and Environmental Microbiology

    Article Title: Characterization of Paenibacillus curdlanolyticus B-6 Xyn10D, a Xylanase That Contains a Family 3 Carbohydrate-Binding Module ▿ B-6 Xyn10D, a Xylanase That Contains a Family 3 Carbohydrate-Binding Module ▿ †

    doi: 10.1128/AEM.00226-11

    Figure Lengend Snippet: Adsorption of rCBM to Avicel and insoluble xylan preparations. rCBM was incubated with Avicel (A), an insoluble fraction of oat spelt xylan (B), and insoluble wheat arabinoxylan (C). After centrifugation, proteins in the supernatant (lane 1), wash (lane 2), and precipitate (lane 3) fractions were analyzed by SDS-PAGE. In panel D, BSA as a control protein was incubated with an insoluble fraction of oat spelt xylan, and each fraction was subjected to SDS-PAGE. In panels A to C, 15% polyacrylamide gels were used. In panel D, a 7.5% polyacrylamide gel was used.

    Article Snippet: They were most active toward rye arabinoxylan.

    Techniques: Adsorption, Incubation, Centrifugation, SDS Page

    Expression of r Tr XYNII in Y. lipolytica . a Screening of Y. lipolytica transformants on agar plates containing 0.2% w/v AZCL-arabinoxylan: lane 1, Y. lipolytica -control; lane 2 to 3, Y. lipolytica expressing r Tr XYNII and rh Tr XYNII respectively; L4, YLC8; b r Tr XYNII production on YTD medium versus time; c SDS-PAGE analysis of the culture supernatant of Y. lipolytica control strain expressing empty plasmid and the recombinant strain expressing rh Tr XYNII; d SDS-PAGE analysis of the rh Tr XYNII after purification, protein marker (lane 1) and rh Tr XYNII (lane 2)

    Journal: Biotechnology for Biofuels

    Article Title: Expressing accessory proteins in cellulolytic Yarrowia lipolytica to improve the conversion yield of recalcitrant cellulose

    doi: 10.1186/s13068-017-0990-y

    Figure Lengend Snippet: Expression of r Tr XYNII in Y. lipolytica . a Screening of Y. lipolytica transformants on agar plates containing 0.2% w/v AZCL-arabinoxylan: lane 1, Y. lipolytica -control; lane 2 to 3, Y. lipolytica expressing r Tr XYNII and rh Tr XYNII respectively; L4, YLC8; b r Tr XYNII production on YTD medium versus time; c SDS-PAGE analysis of the culture supernatant of Y. lipolytica control strain expressing empty plasmid and the recombinant strain expressing rh Tr XYNII; d SDS-PAGE analysis of the rh Tr XYNII after purification, protein marker (lane 1) and rh Tr XYNII (lane 2)

    Article Snippet: The detection of xylanase activity in solid YNB medium was achieved by incorporating 2.0% w/v AZCL-arabinoxylan (Megazyme).

    Techniques: Expressing, SDS Page, Plasmid Preparation, Recombinant, Purification, Marker

    Hydrolysis of water-soluble wheat arabinoxylan by purified rPcAxe. 1, Substrate incubated without enzyme for 24 h (control); 2, hydrolysate treated with rPcAxe for 24 h; 3, arabinose standard; 4, xylose standard. Hydrolysis reactions were performed at 37 °C in buffer consisting of mixtures of 0.1 M citric acid and 0.2 M sodium hydrogen phosphate (pH 7.0) for 24 h with 1% (w/v) water-soluble wheat arabinoxylan loading. The amount of arabinose and xylose released was determined using HPLC. Values are the means and standard deviations of triplicate experiments

    Journal: Microbial Cell Factories

    Article Title: A novel bifunctional acetyl xylan esterase/arabinofuranosidase from Penicillium chrysogenum P33 enhances enzymatic hydrolysis of lignocellulose

    doi: 10.1186/s12934-017-0777-7

    Figure Lengend Snippet: Hydrolysis of water-soluble wheat arabinoxylan by purified rPcAxe. 1, Substrate incubated without enzyme for 24 h (control); 2, hydrolysate treated with rPcAxe for 24 h; 3, arabinose standard; 4, xylose standard. Hydrolysis reactions were performed at 37 °C in buffer consisting of mixtures of 0.1 M citric acid and 0.2 M sodium hydrogen phosphate (pH 7.0) for 24 h with 1% (w/v) water-soluble wheat arabinoxylan loading. The amount of arabinose and xylose released was determined using HPLC. Values are the means and standard deviations of triplicate experiments

    Article Snippet: After 24 h of hydrolysis, rPcAxe released 3.16 μg/mL arabinose from wheat arabinoxylan, which was 0.13% of the total arabinose in the substrate.

    Techniques: Purification, Incubation, High Performance Liquid Chromatography

    Summary of the steps involved in CHD development and the proposed roles played by plant-based diets in preventing CHD. CHD development can be divided into the preocclusion and postocclusion steps. Traditionally, plant-based diets have been thought to delay artery occlusion through inhibition of atherosclerosis (preocclusion steps). Here, we demonstrated that plant-based diets protect the heart after the onset of occlusion (postocclusion steps); when we consume plant-based diets, we also ingest plant cell wall polysaccharides constituting the diets. Arabinose and xylose generated from the hydrolysis of polysaccharides by microbiota inhabiting the large intestine and absorbed into the body protect the heart against ischaemic injury. Subsequently, they may also be involved in inhibiting the development of heart failure through mitigation of adverse left-ventricular remodelling (See the text for more details). AGP, arabinogalactan-peptide; AX, arabinoxylan; XG, xyloglucan; DF, dietary fibres; SCFA, short-chain fatty acids; MI, myocardial infarction.

    Journal: Scientific Reports

    Article Title: Plant-based foods containing cell wall polysaccharides rich in specific active monosaccharides protect against myocardial injury in rat myocardial infarction models

    doi: 10.1038/srep38728

    Figure Lengend Snippet: Summary of the steps involved in CHD development and the proposed roles played by plant-based diets in preventing CHD. CHD development can be divided into the preocclusion and postocclusion steps. Traditionally, plant-based diets have been thought to delay artery occlusion through inhibition of atherosclerosis (preocclusion steps). Here, we demonstrated that plant-based diets protect the heart after the onset of occlusion (postocclusion steps); when we consume plant-based diets, we also ingest plant cell wall polysaccharides constituting the diets. Arabinose and xylose generated from the hydrolysis of polysaccharides by microbiota inhabiting the large intestine and absorbed into the body protect the heart against ischaemic injury. Subsequently, they may also be involved in inhibiting the development of heart failure through mitigation of adverse left-ventricular remodelling (See the text for more details). AGP, arabinogalactan-peptide; AX, arabinoxylan; XG, xyloglucan; DF, dietary fibres; SCFA, short-chain fatty acids; MI, myocardial infarction.

    Article Snippet: Arabinan (sugar beet) (arabinose:galactose:rhamnose: galacturonic acid = 88:3:2:7), galactan (potato) (arabinose:galactose:rhamnose: galacturonic acid = 87:3:3:7), galacturonan (citrus pectin) (galacturonic acid:xylose:galactose:rhamnose = 96:1:1:1.2), β-glucan (barley), arabinoxylan (wheat) (arabinose:xylose = 38:62), glucomannan (Konjac) (glucose:mannose = 40:60), and xyloglucan (tamarind seed) (xylose:glucose:arabinose:galactose = 34:45:3:18) were purchased from Megazyme.

    Techniques: Inhibition, Generated

    Foods containing specific monosaccharide-rich constituents protect against SISR injury. ( a ) Infarct size for various wheat constituents in the SISR model. The doses for arabinoxylan and β-glucan (10 mg/kg/day) were chosen from Supplementary Fig. S7 . The dose for cellulose (100 mg/kg/day) that accounts for 25% of 400 mg/kg/day WE was chosen as the excessive dose because cellulose was not soluble in water. The doses for starch and gluten were chosen by assuming that WE consists of only starch or gluten, based on the fact that starch and protein, which consists mainly of gluten, account for 60–75% and 10–18% of the wheat grains, respectively 63 . The dose of ferulic acid, the major phenolic acid in wheat, was chosen as the excessive dose because the content of ferulic acid in a WE dose of 400 mg/kg/day was calculated as 0.4 mg/kg/day by assuming that ferulic acid accounts for 0.1% of the wheat grains 24 . ( b ) Infarct size for arabinogalactan-peptide. The range of the doses was calculated to include arabinogalactan-peptide content in wheat flour (0.27–0.38%) 64 , which is less than 2 mg/kg/day of arabinogalactan-peptide in 400 mg/kg/day WE. ( c ) Infarct size for various plant cell wall monosaccharides and polysaccharides. The dose of galactose was chosen as the excessive dose by assuming that arabinogalactan-peptide at 100 mg/kg/day consists only of galactose. Data divisions according to polysaccharide and monosaccharide groups are shown as horizontal lines. ( d ) Design of the SISR experiments with samples administered through tail-vein injection 1 hour before LAD artery ligation. ( e ) Infarct size for arabinose, xylose, galactose, and cyclosporine A. * P

    Journal: Scientific Reports

    Article Title: Plant-based foods containing cell wall polysaccharides rich in specific active monosaccharides protect against myocardial injury in rat myocardial infarction models

    doi: 10.1038/srep38728

    Figure Lengend Snippet: Foods containing specific monosaccharide-rich constituents protect against SISR injury. ( a ) Infarct size for various wheat constituents in the SISR model. The doses for arabinoxylan and β-glucan (10 mg/kg/day) were chosen from Supplementary Fig. S7 . The dose for cellulose (100 mg/kg/day) that accounts for 25% of 400 mg/kg/day WE was chosen as the excessive dose because cellulose was not soluble in water. The doses for starch and gluten were chosen by assuming that WE consists of only starch or gluten, based on the fact that starch and protein, which consists mainly of gluten, account for 60–75% and 10–18% of the wheat grains, respectively 63 . The dose of ferulic acid, the major phenolic acid in wheat, was chosen as the excessive dose because the content of ferulic acid in a WE dose of 400 mg/kg/day was calculated as 0.4 mg/kg/day by assuming that ferulic acid accounts for 0.1% of the wheat grains 24 . ( b ) Infarct size for arabinogalactan-peptide. The range of the doses was calculated to include arabinogalactan-peptide content in wheat flour (0.27–0.38%) 64 , which is less than 2 mg/kg/day of arabinogalactan-peptide in 400 mg/kg/day WE. ( c ) Infarct size for various plant cell wall monosaccharides and polysaccharides. The dose of galactose was chosen as the excessive dose by assuming that arabinogalactan-peptide at 100 mg/kg/day consists only of galactose. Data divisions according to polysaccharide and monosaccharide groups are shown as horizontal lines. ( d ) Design of the SISR experiments with samples administered through tail-vein injection 1 hour before LAD artery ligation. ( e ) Infarct size for arabinose, xylose, galactose, and cyclosporine A. * P

    Article Snippet: Arabinan (sugar beet) (arabinose:galactose:rhamnose: galacturonic acid = 88:3:2:7), galactan (potato) (arabinose:galactose:rhamnose: galacturonic acid = 87:3:3:7), galacturonan (citrus pectin) (galacturonic acid:xylose:galactose:rhamnose = 96:1:1:1.2), β-glucan (barley), arabinoxylan (wheat) (arabinose:xylose = 38:62), glucomannan (Konjac) (glucose:mannose = 40:60), and xyloglucan (tamarind seed) (xylose:glucose:arabinose:galactose = 34:45:3:18) were purchased from Megazyme.

    Techniques: Injection, Ligation

    Thin-layer chromatogram of various arabinoxylans and an arabinan before (controls) and after exposure to purified ArfI. Lanes 1 and 19, xylose, xylobiose, xylotriose, xylotetraose, and xylopentaose xylooligosaccharide standards; lanes 2 and 18, arabinose standard; lane 3, rye arabinoxylan plus ArfI; lane 4, rye arabinoxylan control; lane 5, wheat arabinoxylan plus ArfI; lane 6, wheat arabinoxylan control; lane 7, sugar beet arabinan plus ArfI; lane 8, sugar beet arabinan control; lane 9, arabinose and xylose standards; lane 10, CCXA plus ArfI; lane 11, CCXA control; lane 12, CCXB plus ArfI; lane 13, CCXB control; lane 14, CCX plus ArfI; lane 15, CCX control; lane 16, oat spelt arabinoxylan plus ArfI; lane 17, oat spelt arabinoxylan control. A, arabinose; X 1 , xylose; X 2 , xylobiose; X 3 , xylotriose; X 4 , xylotetraose; X 5 , xylopentaose.

    Journal: Applied and Environmental Microbiology

    Article Title: Purification and Properties of ArfI, an ?-l-Arabinofuranosidase from Cytophaga xylanolytica †

    doi:

    Figure Lengend Snippet: Thin-layer chromatogram of various arabinoxylans and an arabinan before (controls) and after exposure to purified ArfI. Lanes 1 and 19, xylose, xylobiose, xylotriose, xylotetraose, and xylopentaose xylooligosaccharide standards; lanes 2 and 18, arabinose standard; lane 3, rye arabinoxylan plus ArfI; lane 4, rye arabinoxylan control; lane 5, wheat arabinoxylan plus ArfI; lane 6, wheat arabinoxylan control; lane 7, sugar beet arabinan plus ArfI; lane 8, sugar beet arabinan control; lane 9, arabinose and xylose standards; lane 10, CCXA plus ArfI; lane 11, CCXA control; lane 12, CCXB plus ArfI; lane 13, CCXB control; lane 14, CCX plus ArfI; lane 15, CCX control; lane 16, oat spelt arabinoxylan plus ArfI; lane 17, oat spelt arabinoxylan control. A, arabinose; X 1 , xylose; X 2 , xylobiose; X 3 , xylotriose; X 4 , xylotetraose; X 5 , xylopentaose.

    Article Snippet: Xylobiose, xylotriose, xylotetraose, xylopentaose, rye and wheat arabinoxylans, and sugar beet arabinan were obtained from Megazyme, Inc. (Sydney, Australia).

    Techniques: Purification

    Relative level of xylanase transcripts in P. cellulosa cultured on wheat arabinoxylan. (A) At various times RNA was extracted and subjected to Northern hybridization with probes that recognized mRNA derived from xyn10A (♦), xyn10B (□), xyn10C (•), xyn10D (▪), xyn11A (▾), and xyn11B (▴). The signal was quantified by using a phosphorimager. (B) Growth curve of the bacterium.

    Journal: Journal of Bacteriology

    Article Title: Evidence for Temporal Regulation of the Two Pseudomonas cellulosa Xylanases Belonging to Glycoside Hydrolase Family 11

    doi: 10.1128/JB.184.15.4124-4133.2002

    Figure Lengend Snippet: Relative level of xylanase transcripts in P. cellulosa cultured on wheat arabinoxylan. (A) At various times RNA was extracted and subjected to Northern hybridization with probes that recognized mRNA derived from xyn10A (♦), xyn10B (□), xyn10C (•), xyn10D (▪), xyn11A (▾), and xyn11B (▴). The signal was quantified by using a phosphorimager. (B) Growth curve of the bacterium.

    Article Snippet: The data revealed two transconjugants that displayed low levels of xylanase activity, as judged by clear haloes when grown on media containing Azo-wheat arabinoxylan.

    Techniques: Cell Culture, Northern Blot, Hybridization, Derivative Assay

    Physical map of xyn11A/xyn11B locus. The positions of the cleavage sites for the following restriction enzymes are as follows: Bgl II (B), Dra I (D), Eco RI (EI), Eco RV (EV), Hin dIII (H), Sma I (S), and Sac II (Sa). The solid arrows show the extent and orientation of the genes encoding Xyn11A ( xyn11A ) and Xyn11B ( xyn11B ). The lines denote the region of pseudomonad DNA present in the plasmids, which were constructed as described in Materials and Methods. The capacity of the plasmids to encode a functional xylanase was determined by plating E. coli containing these DNA molecules on either Blue-Xylan or Azo-wheat arabinoxylan. Clear haloes surrounding the colonies are indicative of xylanase activity (+); no halo indicates the enzyme is absent (−). The modules in the schematic of Xyn11A and Xyn11B are as follows: signal peptide (▥), catalytic domain (▨), linker sequence (▪), family 4 carbohydrate esterase module ( ), xylan-binding CBM (▤), and family 10 CBM (▧).

    Journal: Journal of Bacteriology

    Article Title: Evidence for Temporal Regulation of the Two Pseudomonas cellulosa Xylanases Belonging to Glycoside Hydrolase Family 11

    doi: 10.1128/JB.184.15.4124-4133.2002

    Figure Lengend Snippet: Physical map of xyn11A/xyn11B locus. The positions of the cleavage sites for the following restriction enzymes are as follows: Bgl II (B), Dra I (D), Eco RI (EI), Eco RV (EV), Hin dIII (H), Sma I (S), and Sac II (Sa). The solid arrows show the extent and orientation of the genes encoding Xyn11A ( xyn11A ) and Xyn11B ( xyn11B ). The lines denote the region of pseudomonad DNA present in the plasmids, which were constructed as described in Materials and Methods. The capacity of the plasmids to encode a functional xylanase was determined by plating E. coli containing these DNA molecules on either Blue-Xylan or Azo-wheat arabinoxylan. Clear haloes surrounding the colonies are indicative of xylanase activity (+); no halo indicates the enzyme is absent (−). The modules in the schematic of Xyn11A and Xyn11B are as follows: signal peptide (▥), catalytic domain (▨), linker sequence (▪), family 4 carbohydrate esterase module ( ), xylan-binding CBM (▤), and family 10 CBM (▧).

    Article Snippet: The data revealed two transconjugants that displayed low levels of xylanase activity, as judged by clear haloes when grown on media containing Azo-wheat arabinoxylan.

    Techniques: Construct, Functional Assay, Activity Assay, Sequencing, Binding Assay

    Northern hybridization of P. cellulosa RNA with xylanase-specific probes. An example of Northern hybridization of P. cellulosa RNA, derived from the bacterium cultured on wheat arabinoxylan minimal medium, is shown. The blots were probed with the region of xyn11A encoding the catalytic domain ( xyn11A CD) and xylan-binding CBM ( xyn11A XBD) of Xyn11A, the region of xyn11B that encodes the xylan binding CBM of Xyn11B ( xyn11B XBD), and full-length xyn10D. The time and optical density of the cultures from which the RNA was extracted are shown, as is the RNA electrophoresed on a 1.2% agarose gel and stained with ethidium bromide (RNA).

    Journal: Journal of Bacteriology

    Article Title: Evidence for Temporal Regulation of the Two Pseudomonas cellulosa Xylanases Belonging to Glycoside Hydrolase Family 11

    doi: 10.1128/JB.184.15.4124-4133.2002

    Figure Lengend Snippet: Northern hybridization of P. cellulosa RNA with xylanase-specific probes. An example of Northern hybridization of P. cellulosa RNA, derived from the bacterium cultured on wheat arabinoxylan minimal medium, is shown. The blots were probed with the region of xyn11A encoding the catalytic domain ( xyn11A CD) and xylan-binding CBM ( xyn11A XBD) of Xyn11A, the region of xyn11B that encodes the xylan binding CBM of Xyn11B ( xyn11B XBD), and full-length xyn10D. The time and optical density of the cultures from which the RNA was extracted are shown, as is the RNA electrophoresed on a 1.2% agarose gel and stained with ethidium bromide (RNA).

    Article Snippet: The data revealed two transconjugants that displayed low levels of xylanase activity, as judged by clear haloes when grown on media containing Azo-wheat arabinoxylan.

    Techniques: Northern Blot, Hybridization, Derivative Assay, Cell Culture, Binding Assay, Agarose Gel Electrophoresis, Staining

    Schematic diagram of the domain architecture of XynB and its mutants. The specific activities of XynB and its mutants toward 30 mg/ml arabinoxylan or beechwood xylan were determined at 40°C and pH 7.0. The data shown in the graph are from triplicate experiments (means ± standard deviations [SD]). ND indicates that enzyme activity was not detectable.

    Journal: Applied and Environmental Microbiology

    Article Title: A New Group of Modular Xylanases in Glycoside Hydrolase Family 8 from Marine Bacteria

    doi: 10.1128/AEM.01785-18

    Figure Lengend Snippet: Schematic diagram of the domain architecture of XynB and its mutants. The specific activities of XynB and its mutants toward 30 mg/ml arabinoxylan or beechwood xylan were determined at 40°C and pH 7.0. The data shown in the graph are from triplicate experiments (means ± standard deviations [SD]). ND indicates that enzyme activity was not detectable.

    Article Snippet: Insoluble beechwood xylan was purchased from Sigma (USA), and soluble wheat arabinoxylan (low viscosity) was obtained from Megazyme (Ireland).

    Techniques: Activity Assay

    Release of ferulic acid from (A) 1% wheat arabinoxylan ( WAX ) and (B) 1% sugar beet pectin ( SBP ) using AtFaeD with and without pre‐treatment with commercial xylanase and endopolygalacturonase, respectively. WAX was also co‐incubated with AtFaeD and commercial xylanase. Mean values and standard deviations from three replicate experiments are presented.

    Journal: Microbial Biotechnology

    Article Title: Characterization of a feruloyl esterase from Aspergillus terreus facilitates the division of fungal enzymes from Carbohydrate Esterase family 1 of the carbohydrate‐active enzymes (CAZy) database

    doi: 10.1111/1751-7915.13273

    Figure Lengend Snippet: Release of ferulic acid from (A) 1% wheat arabinoxylan ( WAX ) and (B) 1% sugar beet pectin ( SBP ) using AtFaeD with and without pre‐treatment with commercial xylanase and endopolygalacturonase, respectively. WAX was also co‐incubated with AtFaeD and commercial xylanase. Mean values and standard deviations from three replicate experiments are presented.

    Article Snippet: Hydrolytic activity of A. ferreus FaeD towards polysaccharides Aspergillus terreus FaeD activity was determined towards insoluble wheat arabinoxylan (WAX; Megazyme) and sugar beet pectin (SBP; Pectin Betapec RU301, Herbstreith & Fox KG).

    Techniques: Incubation

    Oligosaccharide content of wheat arabinoxylan ( WAX ) before and after treatment with commercial xylanase and AtFaeD. Vertical error bars represent standard deviations of three replicate experiments.

    Journal: Microbial Biotechnology

    Article Title: Characterization of a feruloyl esterase from Aspergillus terreus facilitates the division of fungal enzymes from Carbohydrate Esterase family 1 of the carbohydrate‐active enzymes (CAZy) database

    doi: 10.1111/1751-7915.13273

    Figure Lengend Snippet: Oligosaccharide content of wheat arabinoxylan ( WAX ) before and after treatment with commercial xylanase and AtFaeD. Vertical error bars represent standard deviations of three replicate experiments.

    Article Snippet: Hydrolytic activity of A. ferreus FaeD towards polysaccharides Aspergillus terreus FaeD activity was determined towards insoluble wheat arabinoxylan (WAX; Megazyme) and sugar beet pectin (SBP; Pectin Betapec RU301, Herbstreith & Fox KG).

    Techniques:

    HPAEC analysis of end products released by endo-acting P. anserina hemicellulases. (A) Ivory nut mannan hydrolysis by Pa Man5A and Pa Man26A: mannose (M1), mannobiose (M2), and mannotriose (M3). (B) Wheat arabinoxylan hydrolysis by Pa Xyn11A: xylose (X1),

    Journal: Applied and Environmental Microbiology

    Article Title: Podospora anserina Hemicellulases Potentiate the Trichoderma reesei Secretome for Saccharification of Lignocellulosic Biomass ▿

    doi: 10.1128/AEM.01761-10

    Figure Lengend Snippet: HPAEC analysis of end products released by endo-acting P. anserina hemicellulases. (A) Ivory nut mannan hydrolysis by Pa Man5A and Pa Man26A: mannose (M1), mannobiose (M2), and mannotriose (M3). (B) Wheat arabinoxylan hydrolysis by Pa Xyn11A: xylose (X1),

    Article Snippet: One unit of endo-glycosidase activity was defined as the amount of protein that released 1 μmol of sugar monomer per min. Low-viscosity wheat arabinoxylan, konjac glucomannan, carob galactomannan, ivory nut mannan, sugar beet arabinan, and debranched arabinan were purchased from Megazyme International (Wicklow, Ireland).

    Techniques: