arabidopsis seedlings Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Thermo Fisher transgenic arabidopsis seedlings
    The antioxidant enzyme activities and MDA contents in <t>transgenic</t> seedlings. The typical antioxidant enzymes activities (CAT, SOD) and MDA contents were examined. (A) Physiological activity of <t>Arabidopsis</t> seedlings under different treatments after 3 d chilling stress. (B) Physiological activity of Arabidopsis seedlings under different treatments after 2 d recovery period from chilling stress. *Significant difference from WT at α=0.05, LSD test.
    Transgenic Arabidopsis Seedlings, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transgenic arabidopsis seedlings/product/Thermo Fisher
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    transgenic arabidopsis seedlings - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    99
    Millipore arabidopsis seedlings
    CML42 elicitation and its functional role in S. littoralis herbivory. A, CML42 -treated leaves of <t>Arabidopsis</t> after 30, 45, 60, and 90 min of treatment. Leaves were elicited by pattern wheel wounding and subsequently treating the
    Arabidopsis Seedlings, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 390 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/arabidopsis seedlings/product/Millipore
    Average 99 stars, based on 390 article reviews
    Price from $9.99 to $1999.99
    arabidopsis seedlings - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    85
    Medicago frozen arabidopsis seedlings
    Two-dimensional 1 H- 13 C HSQC NMR spectrum of sucrose from the BMRB (red) overlaid onto an aqueous whole-plant extract from <t>Arabidopsis</t> thaliana (blue). Black boxes indicate long-range proton carbon couplings used to validate the assignment.
    Frozen Arabidopsis Seedlings, supplied by Medicago, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/frozen arabidopsis seedlings/product/Medicago
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    frozen arabidopsis seedlings - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    93
    Collaborative Drug Discovery Inc a thaliana seedlings
    Red lines represent endogenous biological chemiluminescence from germinating A. <t>thaliana</t> seedlings samples. Green line represents signal from agar. The lines are produced from the raw data using smoothed LOESS algorithm. Note that the photodetector noise (mean counts/s = 12.5) is included in the signals.
    A Thaliana Seedlings, supplied by Collaborative Drug Discovery Inc, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a thaliana seedlings/product/Collaborative Drug Discovery Inc
    Average 93 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    a thaliana seedlings - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    92
    Nikon arabidopsis seedlings
    Biphasic [Ca 2+ ] c transient induced by gravistimulation in <t>Arabidopsis</t> seedlings. A, Typical time course of changes in luminescence ratio induced by gravistimulation (±180° rotation). Approximately 40 Arabidopsis seedlings grown
    Arabidopsis Seedlings, supplied by Nikon, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/arabidopsis seedlings/product/Nikon
    Average 92 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    arabidopsis seedlings - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    90
    Duchefa arabidopsis seedlings
    Comparison of the root hair growth rate and root hair calcium oscillation frequencies present in wild-type <t>Arabidopsis</t> seedling grown in control conditions or in a medium supplemented with 50 nM NAA. Germinated seedlings (36–40 h after vernalization) were transferred from MS/2 standard plates to FEP tubes supplemented or not with 50 nM NAA, and seedlings were visualized after 6 d through the light sheet set-up. (A) Statistical analysis of root hair growth velocity. Values are means ± SD ( n CNT = 43 and n NAA = 31). The two main peaks in the PSD of the control (B) and NAA-treated samples (C) ( n CNT = 43 and n NAA = 31) were plotted and clustered through a k -mean algorithm with k = 2. The low frequency cluster is shown by blue circles, while the high frequency cluster is shown by orange triangles. Black crosses are used to visualize the computed barycenters. The mean and SE of the two main frequencies (LF, low frequency; HF, high frequency) of the two populations is presented in (D).
    Arabidopsis Seedlings, supplied by Duchefa, used in various techniques. Bioz Stars score: 90/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/arabidopsis seedlings/product/Duchefa
    Average 90 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    arabidopsis seedlings - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    93
    Carl Zeiss arabidopsis seedlings
    Identification of the NLS in NPR1 by Mutagenesis. (A) Subcellular localization of GFP, NPR1-GFP, and npr1Δ57-GFP (a mutant lacking the C-terminal 57 amino acids of NPR1). GFP fluorescence (top images) and differential interference contrast images (middle images) of onion epidermal cells were compared to show the subcellular localization of GFP (cytoplasmic and nuclear), NPR1-GFP (nuclear), and npr1Δ57-GFP (cytoplasmic). Confocal GFP images (bottom images) were captured from 7-day-old transgenic <t>Arabidopsis</t> seedlings expressing GFP, NPR1-GFP, or npr1Δ57-GFP grown on MS-INA. GFP fluorescence is shown in the green channel; differential interference contrast images are shown in the red channel. (B) Sequence of the C-terminal 57 amino acids of NPR1. The two potential NLSs are underlined. Point mutations in the amino acids shown in red had a marked effect on NPR1-GFP nuclear localization, whereas mutations in the amino acids shown in italics had no detectable effect on nuclear localization. Mutations in all five amino acids shown in red resulted in the exclusive cytoplasmic localization of the fusion protein npr1nls-GFP. (C) Cytoplasmic localization of npr1nls-GFP in leaf epidermal cells of transgenic seedlings.
    Arabidopsis Seedlings, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 93/100, based on 134 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/arabidopsis seedlings/product/Carl Zeiss
    Average 93 stars, based on 134 article reviews
    Price from $9.99 to $1999.99
    arabidopsis seedlings - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

    92
    Thermo Fisher arabidopsis seedlings
    Complementation and Cosuppression Effect of the 35S : FLAG-CAND1 Transgene. (A) Expression of the FLAG-CAND1 fusion protein. Flower protein extracts from wild-type <t>Arabidopsis,</t> cand1-1 mutant, and various 35S : FLAG-CAND1 transgenic Arabidopsis lines (line numbers labeled at the top) were subjected to immunoblot analysis with anti-CAND1 and anti-RPN6 antibodies. Lines marked as +/− are heterozygous for cand1-1 mutation, and lines marked as −/− are homozygous for the cand1-1 mutation. Arrowheads indicate protein positions. The asterisk marks a cross-reacting band. RPN6 is used as a loading control. (B) Comparison of partial complementation phenotypes and cosuppression phenotypes. An arrow is put at the top of each line with the name as labeled above. Plants shown next to the indicated lines are other transgenic Arabidopsis lines that are heterozygous for the cand1-1 mutation. Pictures were taken when the plants were 7 weeks old.
    Arabidopsis Seedlings, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 680 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/arabidopsis seedlings/product/Thermo Fisher
    Average 92 stars, based on 680 article reviews
    Price from $9.99 to $1999.99
    arabidopsis seedlings - by Bioz Stars, 2020-07
    92/100 stars
      Buy from Supplier

    90
    Beyotime arabidopsis seedlings
    Constitutively expressing TaBASS2 represses ABI4 expression. a , b The expression levels of ABI4 and HKT1;1 in 12-day-old wild-type and two 35S::TaBASS2 transgenic lines (OE1 and OE3). Error bars represent the standard errors ( n = 3), with each replicate comprising at least 12 plants. The expression levels were determined by RT-qPCR using AtACT2 in <t>Arabidopsis</t> as the internal control c , d The Na + contents in the shoot and root tissue from ten-day-old wild-type and OE lines. Error bars represent the standard errors ( n = 3), with each replicate comprising at least 200 plants. Columns labeled with an asterisk indicate means differing significantly from the WT result ( P
    Arabidopsis Seedlings, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/arabidopsis seedlings/product/Beyotime
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    arabidopsis seedlings - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    88
    Dualsystems Biotech 6 d old arabidopsis seedlings
    Constitutively expressing TaBASS2 represses ABI4 expression. a , b The expression levels of ABI4 and HKT1;1 in 12-day-old wild-type and two 35S::TaBASS2 transgenic lines (OE1 and OE3). Error bars represent the standard errors ( n = 3), with each replicate comprising at least 12 plants. The expression levels were determined by RT-qPCR using AtACT2 in <t>Arabidopsis</t> as the internal control c , d The Na + contents in the shoot and root tissue from ten-day-old wild-type and OE lines. Error bars represent the standard errors ( n = 3), with each replicate comprising at least 200 plants. Columns labeled with an asterisk indicate means differing significantly from the WT result ( P
    6 D Old Arabidopsis Seedlings, supplied by Dualsystems Biotech, used in various techniques. Bioz Stars score: 88/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/6 d old arabidopsis seedlings/product/Dualsystems Biotech
    Average 88 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    6 d old arabidopsis seedlings - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    88
    PhytoTechnology Laboratories a thaliana col 0 seedlings
    Constitutively expressing TaBASS2 represses ABI4 expression. a , b The expression levels of ABI4 and HKT1;1 in 12-day-old wild-type and two 35S::TaBASS2 transgenic lines (OE1 and OE3). Error bars represent the standard errors ( n = 3), with each replicate comprising at least 12 plants. The expression levels were determined by RT-qPCR using AtACT2 in <t>Arabidopsis</t> as the internal control c , d The Na + contents in the shoot and root tissue from ten-day-old wild-type and OE lines. Error bars represent the standard errors ( n = 3), with each replicate comprising at least 200 plants. Columns labeled with an asterisk indicate means differing significantly from the WT result ( P
    A Thaliana Col 0 Seedlings, supplied by PhytoTechnology Laboratories, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a thaliana col 0 seedlings/product/PhytoTechnology Laboratories
    Average 88 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    a thaliana col 0 seedlings - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    91
    Eppendorf AG arabidopsis thaliana seedling
    Constitutively expressing TaBASS2 represses ABI4 expression. a , b The expression levels of ABI4 and HKT1;1 in 12-day-old wild-type and two 35S::TaBASS2 transgenic lines (OE1 and OE3). Error bars represent the standard errors ( n = 3), with each replicate comprising at least 12 plants. The expression levels were determined by RT-qPCR using AtACT2 in <t>Arabidopsis</t> as the internal control c , d The Na + contents in the shoot and root tissue from ten-day-old wild-type and OE lines. Error bars represent the standard errors ( n = 3), with each replicate comprising at least 200 plants. Columns labeled with an asterisk indicate means differing significantly from the WT result ( P
    Arabidopsis Thaliana Seedling, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/arabidopsis thaliana seedling/product/Eppendorf AG
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    arabidopsis thaliana seedling - by Bioz Stars, 2020-07
    91/100 stars
      Buy from Supplier

    89
    Medicago arabidopsis thaliana seedlings
    Relative abundance of ACS and ACO multigene family. Relative abundance of ACS (A-B) and ACO (C-D) multigene family members in roots and leaves of 3 weeks old <t>Arabidopsis</t> <t>thaliana</t> plants exposed for 0, 24 or 72 h to either 5 or 10 μM CdSO 4 or grown under control conditions in a hydroponic culture system. Data represent mean abundance of at least 4 biological replicates relative to the control (0 h, 0 μM CdSO 4 ) and with the abundance of the most highly expressed family member set at 1 under the control condition. (A) Relative abundance of ACS multigene family members in roots. (B) Relative abundance of ACS multigene family members in leaves. (C) Relative abundance of ACO multigene family members in roots. (D) Relative abundance of ACO multigene family members in leaves.
    Arabidopsis Thaliana Seedlings, supplied by Medicago, used in various techniques. Bioz Stars score: 89/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/arabidopsis thaliana seedlings/product/Medicago
    Average 89 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    arabidopsis thaliana seedlings - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

    84
    Hybrigenics a thaliana seedling cdna library
    Relative abundance of ACS and ACO multigene family. Relative abundance of ACS (A-B) and ACO (C-D) multigene family members in roots and leaves of 3 weeks old <t>Arabidopsis</t> <t>thaliana</t> plants exposed for 0, 24 or 72 h to either 5 or 10 μM CdSO 4 or grown under control conditions in a hydroponic culture system. Data represent mean abundance of at least 4 biological replicates relative to the control (0 h, 0 μM CdSO 4 ) and with the abundance of the most highly expressed family member set at 1 under the control condition. (A) Relative abundance of ACS multigene family members in roots. (B) Relative abundance of ACS multigene family members in leaves. (C) Relative abundance of ACO multigene family members in roots. (D) Relative abundance of ACO multigene family members in leaves.
    A Thaliana Seedling Cdna Library, supplied by Hybrigenics, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/a thaliana seedling cdna library/product/Hybrigenics
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    a thaliana seedling cdna library - by Bioz Stars, 2020-07
    84/100 stars
      Buy from Supplier

    88
    Caisson Labs arabidopsis col 0 seedlings
    Relative abundance of ACS and ACO multigene family. Relative abundance of ACS (A-B) and ACO (C-D) multigene family members in roots and leaves of 3 weeks old <t>Arabidopsis</t> <t>thaliana</t> plants exposed for 0, 24 or 72 h to either 5 or 10 μM CdSO 4 or grown under control conditions in a hydroponic culture system. Data represent mean abundance of at least 4 biological replicates relative to the control (0 h, 0 μM CdSO 4 ) and with the abundance of the most highly expressed family member set at 1 under the control condition. (A) Relative abundance of ACS multigene family members in roots. (B) Relative abundance of ACS multigene family members in leaves. (C) Relative abundance of ACO multigene family members in roots. (D) Relative abundance of ACO multigene family members in leaves.
    Arabidopsis Col 0 Seedlings, supplied by Caisson Labs, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/arabidopsis col 0 seedlings/product/Caisson Labs
    Average 88 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    arabidopsis col 0 seedlings - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    84
    Hybrigenics ultimate y2h a thaliana seedling library
    Relative abundance of ACS and ACO multigene family. Relative abundance of ACS (A-B) and ACO (C-D) multigene family members in roots and leaves of 3 weeks old <t>Arabidopsis</t> <t>thaliana</t> plants exposed for 0, 24 or 72 h to either 5 or 10 μM CdSO 4 or grown under control conditions in a hydroponic culture system. Data represent mean abundance of at least 4 biological replicates relative to the control (0 h, 0 μM CdSO 4 ) and with the abundance of the most highly expressed family member set at 1 under the control condition. (A) Relative abundance of ACS multigene family members in roots. (B) Relative abundance of ACS multigene family members in leaves. (C) Relative abundance of ACO multigene family members in roots. (D) Relative abundance of ACO multigene family members in leaves.
    Ultimate Y2h A Thaliana Seedling Library, supplied by Hybrigenics, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ultimate y2h a thaliana seedling library/product/Hybrigenics
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ultimate y2h a thaliana seedling library - by Bioz Stars, 2020-07
    84/100 stars
      Buy from Supplier

    99
    Millipore luciferase assays arabidopsis seedlings
    Relative abundance of ACS and ACO multigene family. Relative abundance of ACS (A-B) and ACO (C-D) multigene family members in roots and leaves of 3 weeks old <t>Arabidopsis</t> <t>thaliana</t> plants exposed for 0, 24 or 72 h to either 5 or 10 μM CdSO 4 or grown under control conditions in a hydroponic culture system. Data represent mean abundance of at least 4 biological replicates relative to the control (0 h, 0 μM CdSO 4 ) and with the abundance of the most highly expressed family member set at 1 under the control condition. (A) Relative abundance of ACS multigene family members in roots. (B) Relative abundance of ACS multigene family members in leaves. (C) Relative abundance of ACO multigene family members in roots. (D) Relative abundance of ACO multigene family members in leaves.
    Luciferase Assays Arabidopsis Seedlings, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/luciferase assays arabidopsis seedlings/product/Millipore
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    luciferase assays arabidopsis seedlings - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    88
    Promega 6 d old arabidopsis seedlings
    Characterization of the <t>Arabidopsis</t> sig6 - 2 mutant. A, Genomic Sig6 region (At2g36990) showing exon/intron structure and T-DNA insertion site in exon 5, 1,022 nucleotides downstream from the ATG start codon (top line). The resulting cDNA with the fused exons, but without the 5′ and 3′ untranslated regions, is depicted below. Also given are features of the derived protein. TP, Transit peptide; UR, unconserved region; 1–4, conserved regions for sigma activity. Genomic sequence and cDNA, but not T-DNA, are drawn to scale (scale bar on top). LB, Left border; RB, right border; sul , sulfonamide (sulfadiazine) resistance gene. B, RT-PCR detection of sigma factor transcripts in wild type (WT) and sig6 - 2 mutant. Total RNA was prepared from <t>6-d</t> seedlings, reverse transcribed, and cDNA was amplified using the gene-specific primer pairs as described in “Materials and Methods.” C, Wild-type (WT) and sig6 - 2 mutant phenotype during development (4, 8, 10, 12, 21, and 28 d after sowing).
    6 D Old Arabidopsis Seedlings, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/6 d old arabidopsis seedlings/product/Promega
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    6 d old arabidopsis seedlings - by Bioz Stars, 2020-07
    88/100 stars
      Buy from Supplier

    85
    TaKaRa arabidopsis seedling cdna library
    Characterization of the <t>Arabidopsis</t> sig6 - 2 mutant. A, Genomic Sig6 region (At2g36990) showing exon/intron structure and T-DNA insertion site in exon 5, 1,022 nucleotides downstream from the ATG start codon (top line). The resulting cDNA with the fused exons, but without the 5′ and 3′ untranslated regions, is depicted below. Also given are features of the derived protein. TP, Transit peptide; UR, unconserved region; 1–4, conserved regions for sigma activity. Genomic sequence and cDNA, but not T-DNA, are drawn to scale (scale bar on top). LB, Left border; RB, right border; sul , sulfonamide (sulfadiazine) resistance gene. B, RT-PCR detection of sigma factor transcripts in wild type (WT) and sig6 - 2 mutant. Total RNA was prepared from <t>6-d</t> seedlings, reverse transcribed, and cDNA was amplified using the gene-specific primer pairs as described in “Materials and Methods.” C, Wild-type (WT) and sig6 - 2 mutant phenotype during development (4, 8, 10, 12, 21, and 28 d after sowing).
    Arabidopsis Seedling Cdna Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/arabidopsis seedling cdna library/product/TaKaRa
    Average 85 stars, based on 30 article reviews
    Price from $9.99 to $1999.99
    arabidopsis seedling cdna library - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    85
    Osram 14 d old arabidopsis seedlings
    Characterization of the <t>Arabidopsis</t> sig6 - 2 mutant. A, Genomic Sig6 region (At2g36990) showing exon/intron structure and T-DNA insertion site in exon 5, 1,022 nucleotides downstream from the ATG start codon (top line). The resulting cDNA with the fused exons, but without the 5′ and 3′ untranslated regions, is depicted below. Also given are features of the derived protein. TP, Transit peptide; UR, unconserved region; 1–4, conserved regions for sigma activity. Genomic sequence and cDNA, but not T-DNA, are drawn to scale (scale bar on top). LB, Left border; RB, right border; sul , sulfonamide (sulfadiazine) resistance gene. B, RT-PCR detection of sigma factor transcripts in wild type (WT) and sig6 - 2 mutant. Total RNA was prepared from <t>6-d</t> seedlings, reverse transcribed, and cDNA was amplified using the gene-specific primer pairs as described in “Materials and Methods.” C, Wild-type (WT) and sig6 - 2 mutant phenotype during development (4, 8, 10, 12, 21, and 28 d after sowing).
    14 D Old Arabidopsis Seedlings, supplied by Osram, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/14 d old arabidopsis seedlings/product/Osram
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    14 d old arabidopsis seedlings - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    99
    Qiagen arabidopsis seedlings
    Analysis of the Expression of the <t>Arabidopsis</t> BIO3-BIO1 Locus at the Protein Level and Subcellular Distribution. (A) Immunological detection of BIO3-BIO1 gene products in Arabidopsis . Soluble proteins (40 µg) from aboveground organs from Arabidopsis plants (lane 1) and Arabidopsis cultured cells (lane 2) were separated by SDS-PAGE and analyzed by immunoblotting using affinity-purified antibodies raised against recombinant BIO3-BIO1 (BIO3-BIO1 Ab) or preimmune serum (for negative control). (B) Immunolocalization of BIO3-BIO1 in Arabidopsis . Soluble proteins (50 µg) from total plant extracts (T), chloroplast stroma (St), mitochondrial matrix (Ma), and cytosolic enriched fraction (Cy) were separated by SDS-PAGE and analyzed by immunoblotting as in (A) . Arrows in (A) and (B) point to the BIO3-BIO1 polypeptide band. (C) in Arabidopsis fused to the C terminus of the small subunit of ribulose-1,5-bis-phosphate carboxylase/oxygenase transit peptide (a chloroplastic marker) from Arabidopsis fused to the C terminus of the dihydropterin pyrophosphokinase-dihydropteroate synthase transit peptide (a mitochondrial marker) from pea ( Pisum sativum fused to the C terminus of full-length BIO3-BIO1 (BIO3-BIO1) were introduced into Arabidopsis ; green pseudocolor), and chlorophyll fluorescence (Chlorophyll; red pseudocolor). Bar = 10 µm.
    Arabidopsis Seedlings, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 648 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/arabidopsis seedlings/product/Qiagen
    Average 99 stars, based on 648 article reviews
    Price from $9.99 to $1999.99
    arabidopsis seedlings - by Bioz Stars, 2020-07
    99/100 stars
      Buy from Supplier

    90
    Thermo Fisher d old arabidopsis thaliana seedling rna
    Analysis of the Expression of the <t>Arabidopsis</t> BIO3-BIO1 Locus at the Protein Level and Subcellular Distribution. (A) Immunological detection of BIO3-BIO1 gene products in Arabidopsis . Soluble proteins (40 µg) from aboveground organs from Arabidopsis plants (lane 1) and Arabidopsis cultured cells (lane 2) were separated by SDS-PAGE and analyzed by immunoblotting using affinity-purified antibodies raised against recombinant BIO3-BIO1 (BIO3-BIO1 Ab) or preimmune serum (for negative control). (B) Immunolocalization of BIO3-BIO1 in Arabidopsis . Soluble proteins (50 µg) from total plant extracts (T), chloroplast stroma (St), mitochondrial matrix (Ma), and cytosolic enriched fraction (Cy) were separated by SDS-PAGE and analyzed by immunoblotting as in (A) . Arrows in (A) and (B) point to the BIO3-BIO1 polypeptide band. (C) in Arabidopsis fused to the C terminus of the small subunit of ribulose-1,5-bis-phosphate carboxylase/oxygenase transit peptide (a chloroplastic marker) from Arabidopsis fused to the C terminus of the dihydropterin pyrophosphokinase-dihydropteroate synthase transit peptide (a mitochondrial marker) from pea ( Pisum sativum fused to the C terminus of full-length BIO3-BIO1 (BIO3-BIO1) were introduced into Arabidopsis ; green pseudocolor), and chlorophyll fluorescence (Chlorophyll; red pseudocolor). Bar = 10 µm.
    D Old Arabidopsis Thaliana Seedling Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/d old arabidopsis thaliana seedling rna/product/Thermo Fisher
    Average 90 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    d old arabidopsis thaliana seedling rna - by Bioz Stars, 2020-07
    90/100 stars
      Buy from Supplier

    85
    Duchefa preparation 10 day old arabidopsis col 0 seedlings
    Analysis of the Expression of the <t>Arabidopsis</t> BIO3-BIO1 Locus at the Protein Level and Subcellular Distribution. (A) Immunological detection of BIO3-BIO1 gene products in Arabidopsis . Soluble proteins (40 µg) from aboveground organs from Arabidopsis plants (lane 1) and Arabidopsis cultured cells (lane 2) were separated by SDS-PAGE and analyzed by immunoblotting using affinity-purified antibodies raised against recombinant BIO3-BIO1 (BIO3-BIO1 Ab) or preimmune serum (for negative control). (B) Immunolocalization of BIO3-BIO1 in Arabidopsis . Soluble proteins (50 µg) from total plant extracts (T), chloroplast stroma (St), mitochondrial matrix (Ma), and cytosolic enriched fraction (Cy) were separated by SDS-PAGE and analyzed by immunoblotting as in (A) . Arrows in (A) and (B) point to the BIO3-BIO1 polypeptide band. (C) in Arabidopsis fused to the C terminus of the small subunit of ribulose-1,5-bis-phosphate carboxylase/oxygenase transit peptide (a chloroplastic marker) from Arabidopsis fused to the C terminus of the dihydropterin pyrophosphokinase-dihydropteroate synthase transit peptide (a mitochondrial marker) from pea ( Pisum sativum fused to the C terminus of full-length BIO3-BIO1 (BIO3-BIO1) were introduced into Arabidopsis ; green pseudocolor), and chlorophyll fluorescence (Chlorophyll; red pseudocolor). Bar = 10 µm.
    Preparation 10 Day Old Arabidopsis Col 0 Seedlings, supplied by Duchefa, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/preparation 10 day old arabidopsis col 0 seedlings/product/Duchefa
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    preparation 10 day old arabidopsis col 0 seedlings - by Bioz Stars, 2020-07
    85/100 stars
      Buy from Supplier

    Image Search Results


    The antioxidant enzyme activities and MDA contents in transgenic seedlings. The typical antioxidant enzymes activities (CAT, SOD) and MDA contents were examined. (A) Physiological activity of Arabidopsis seedlings under different treatments after 3 d chilling stress. (B) Physiological activity of Arabidopsis seedlings under different treatments after 2 d recovery period from chilling stress. *Significant difference from WT at α=0.05, LSD test.

    Journal: Journal of Experimental Botany

    Article Title: Maize annexin genes ZmANN33 and ZmANN35 encode proteins that function in cell membrane recovery during seed germination

    doi: 10.1093/jxb/ery452

    Figure Lengend Snippet: The antioxidant enzyme activities and MDA contents in transgenic seedlings. The typical antioxidant enzymes activities (CAT, SOD) and MDA contents were examined. (A) Physiological activity of Arabidopsis seedlings under different treatments after 3 d chilling stress. (B) Physiological activity of Arabidopsis seedlings under different treatments after 2 d recovery period from chilling stress. *Significant difference from WT at α=0.05, LSD test.

    Article Snippet: Seven-day-old WT and transgenic Arabidopsis seedlings grown under normal conditions were chilled at 1 °C for 3 h followed by immediate incubation with 50 µM FM4-64 (a lipophilic probe binding to the outer surface of the PM; Life technologies, USA) for 5 min.

    Techniques: Multiple Displacement Amplification, Transgenic Assay, Activity Assay

    Ectopic expression of ZmANN33/35 promoted seedling recovery from chilling stress. (A) Phenotypes of Arabidopsis seedlings after 2 d of recovery from chilling stress (1 °C). ZmANN33 ectopic expression Arabidopsis seedlings (ZmANN33OE-1, ZmANN33OE-2), ZmANN35 ectopic expression seedlings (ZmANN35OE-7, ZmANN35OE-24) and WT (Col) were grown for 4 d at 23 °C on standard 1/2 MS medium and 1/2 MS medium supplemented with 5 mM EGTA. Then they were subjected to 1 °C for 3 d. After the chilling treatment, two kinds of transgenic seedlings and WT under different treatments were transferred to 23 °C for 2 d recovery. Bar=5 mm. (B, C) Root length and electrolyte leakage of different Arabidopsis seedlings corresponding to (A). *Significant difference from WT at α=0.05, LSD test. (This figure is available in color at JXB online.)

    Journal: Journal of Experimental Botany

    Article Title: Maize annexin genes ZmANN33 and ZmANN35 encode proteins that function in cell membrane recovery during seed germination

    doi: 10.1093/jxb/ery452

    Figure Lengend Snippet: Ectopic expression of ZmANN33/35 promoted seedling recovery from chilling stress. (A) Phenotypes of Arabidopsis seedlings after 2 d of recovery from chilling stress (1 °C). ZmANN33 ectopic expression Arabidopsis seedlings (ZmANN33OE-1, ZmANN33OE-2), ZmANN35 ectopic expression seedlings (ZmANN35OE-7, ZmANN35OE-24) and WT (Col) were grown for 4 d at 23 °C on standard 1/2 MS medium and 1/2 MS medium supplemented with 5 mM EGTA. Then they were subjected to 1 °C for 3 d. After the chilling treatment, two kinds of transgenic seedlings and WT under different treatments were transferred to 23 °C for 2 d recovery. Bar=5 mm. (B, C) Root length and electrolyte leakage of different Arabidopsis seedlings corresponding to (A). *Significant difference from WT at α=0.05, LSD test. (This figure is available in color at JXB online.)

    Article Snippet: Seven-day-old WT and transgenic Arabidopsis seedlings grown under normal conditions were chilled at 1 °C for 3 h followed by immediate incubation with 50 µM FM4-64 (a lipophilic probe binding to the outer surface of the PM; Life technologies, USA) for 5 min.

    Techniques: Expressing, Mass Spectrometry, Transgenic Assay

    CML42 elicitation and its functional role in S. littoralis herbivory. A, CML42 -treated leaves of Arabidopsis after 30, 45, 60, and 90 min of treatment. Leaves were elicited by pattern wheel wounding and subsequently treating the

    Journal: Plant Physiology

    Article Title: CML42-Mediated Calcium Signaling Coordinates Responses to Spodoptera Herbivory and Abiotic Stresses in Arabidopsis 1 Herbivory and Abiotic Stresses in Arabidopsis 1 [W] Herbivory and Abiotic Stresses in Arabidopsis 1 [W] [OA]

    doi: 10.1104/pp.112.198150

    Figure Lengend Snippet: CML42 elicitation and its functional role in S. littoralis herbivory. A, CML42 -treated leaves of Arabidopsis after 30, 45, 60, and 90 min of treatment. Leaves were elicited by pattern wheel wounding and subsequently treating the

    Article Snippet: The root growth inhibition assay was done by growing Arabidopsis seedlings on control, 10, 20, 30, 40, and 50 µ m (Sigma).

    Techniques: Functional Assay

    Multiple stress responses regulated by CML42 . A, Relative concentrations of flavonol glycosides in rosette leaves of 5-week-old untreated Arabidopsis wild-type (black bars) and cml42 (white bars) plants. Relative concentrations of flavonol glycosides

    Journal: Plant Physiology

    Article Title: CML42-Mediated Calcium Signaling Coordinates Responses to Spodoptera Herbivory and Abiotic Stresses in Arabidopsis 1 Herbivory and Abiotic Stresses in Arabidopsis 1 [W] Herbivory and Abiotic Stresses in Arabidopsis 1 [W] [OA]

    doi: 10.1104/pp.112.198150

    Figure Lengend Snippet: Multiple stress responses regulated by CML42 . A, Relative concentrations of flavonol glycosides in rosette leaves of 5-week-old untreated Arabidopsis wild-type (black bars) and cml42 (white bars) plants. Relative concentrations of flavonol glycosides

    Article Snippet: The root growth inhibition assay was done by growing Arabidopsis seedlings on control, 10, 20, 30, 40, and 50 µ m (Sigma).

    Techniques:

    Phytohormone elevation upon S. littoralis herbivory in cml42 plants. Mean ± se ( n (C), and salicylic acid (SA; D) in Arabidopsis wild-type (white bars) and cml42 (black bars) plants fed

    Journal: Plant Physiology

    Article Title: CML42-Mediated Calcium Signaling Coordinates Responses to Spodoptera Herbivory and Abiotic Stresses in Arabidopsis 1 Herbivory and Abiotic Stresses in Arabidopsis 1 [W] Herbivory and Abiotic Stresses in Arabidopsis 1 [W] [OA]

    doi: 10.1104/pp.112.198150

    Figure Lengend Snippet: Phytohormone elevation upon S. littoralis herbivory in cml42 plants. Mean ± se ( n (C), and salicylic acid (SA; D) in Arabidopsis wild-type (white bars) and cml42 (black bars) plants fed

    Article Snippet: The root growth inhibition assay was done by growing Arabidopsis seedlings on control, 10, 20, 30, 40, and 50 µ m (Sigma).

    Techniques:

    Subcellular CML42 localization. A, Visualization of CML42-GFP in leaves of transgenic Arabidopsis plants using confocal laser scanning microscopy. Green fluorescence of the fusion protein is visible in cytoplasm surrounding the chloroplasts (arrowheads)

    Journal: Plant Physiology

    Article Title: CML42-Mediated Calcium Signaling Coordinates Responses to Spodoptera Herbivory and Abiotic Stresses in Arabidopsis 1 Herbivory and Abiotic Stresses in Arabidopsis 1 [W] Herbivory and Abiotic Stresses in Arabidopsis 1 [W] [OA]

    doi: 10.1104/pp.112.198150

    Figure Lengend Snippet: Subcellular CML42 localization. A, Visualization of CML42-GFP in leaves of transgenic Arabidopsis plants using confocal laser scanning microscopy. Green fluorescence of the fusion protein is visible in cytoplasm surrounding the chloroplasts (arrowheads)

    Article Snippet: The root growth inhibition assay was done by growing Arabidopsis seedlings on control, 10, 20, 30, 40, and 50 µ m (Sigma).

    Techniques: Transgenic Assay, Confocal Laser Scanning Microscopy, Fluorescence

    S. littoralis -induced changes in cytosolic calcium concentration ([Ca 2+ ] cyt ) and phytohormones in Arabidopsis. A, Application of 40 µL of S. littoralis (1:1 diluted) to 5-week-old Arabidopsis leaf discs expressing aequorin. Mean ±

    Journal: Plant Physiology

    Article Title: CML42-Mediated Calcium Signaling Coordinates Responses to Spodoptera Herbivory and Abiotic Stresses in Arabidopsis 1 Herbivory and Abiotic Stresses in Arabidopsis 1 [W] Herbivory and Abiotic Stresses in Arabidopsis 1 [W] [OA]

    doi: 10.1104/pp.112.198150

    Figure Lengend Snippet: S. littoralis -induced changes in cytosolic calcium concentration ([Ca 2+ ] cyt ) and phytohormones in Arabidopsis. A, Application of 40 µL of S. littoralis (1:1 diluted) to 5-week-old Arabidopsis leaf discs expressing aequorin. Mean ±

    Article Snippet: The root growth inhibition assay was done by growing Arabidopsis seedlings on control, 10, 20, 30, 40, and 50 µ m (Sigma).

    Techniques: Concentration Assay, Expressing

    levels in cml42 plants. A, Mean ± se in rosette leaves of 5-week-old untreated Arabidopsis wild-type (black bars) and cml42 (white bars) plants. Statistically significant differences between Col-0

    Journal: Plant Physiology

    Article Title: CML42-Mediated Calcium Signaling Coordinates Responses to Spodoptera Herbivory and Abiotic Stresses in Arabidopsis 1 Herbivory and Abiotic Stresses in Arabidopsis 1 [W] Herbivory and Abiotic Stresses in Arabidopsis 1 [W] [OA]

    doi: 10.1104/pp.112.198150

    Figure Lengend Snippet: levels in cml42 plants. A, Mean ± se in rosette leaves of 5-week-old untreated Arabidopsis wild-type (black bars) and cml42 (white bars) plants. Statistically significant differences between Col-0

    Article Snippet: The root growth inhibition assay was done by growing Arabidopsis seedlings on control, 10, 20, 30, 40, and 50 µ m (Sigma).

    Techniques:

    Two-dimensional 1 H- 13 C HSQC NMR spectrum of sucrose from the BMRB (red) overlaid onto an aqueous whole-plant extract from Arabidopsis thaliana (blue). Black boxes indicate long-range proton carbon couplings used to validate the assignment.

    Journal: Analytical chemistry

    Article Title: Fast and Accurate Method for Determining Molar Concentrations of Metabolites in Complex Solutions from Two-Dimensional 1H-13C NMR Spectra

    doi: 10.1021/ac071583z

    Figure Lengend Snippet: Two-dimensional 1 H- 13 C HSQC NMR spectrum of sucrose from the BMRB (red) overlaid onto an aqueous whole-plant extract from Arabidopsis thaliana (blue). Black boxes indicate long-range proton carbon couplings used to validate the assignment.

    Article Snippet: Frozen Arabidopsis seedlings, Medicago sprouts, and Saccharomyces cells were lyophilized for 48 h and homogenized in a coffee grinder.

    Techniques: Nuclear Magnetic Resonance

    Number of observed metabolites in Arabidopsis, Medicago , and Saccharomyces extracts as a function of observed concentration (mM in the NMR tube; N=94). Bars indicate the total number of metabolite occurrences in the three extracts within consecutive 2

    Journal: Analytical chemistry

    Article Title: Fast and Accurate Method for Determining Molar Concentrations of Metabolites in Complex Solutions from Two-Dimensional 1H-13C NMR Spectra

    doi: 10.1021/ac071583z

    Figure Lengend Snippet: Number of observed metabolites in Arabidopsis, Medicago , and Saccharomyces extracts as a function of observed concentration (mM in the NMR tube; N=94). Bars indicate the total number of metabolite occurrences in the three extracts within consecutive 2

    Article Snippet: Frozen Arabidopsis seedlings, Medicago sprouts, and Saccharomyces cells were lyophilized for 48 h and homogenized in a coffee grinder.

    Techniques: Concentration Assay, Nuclear Magnetic Resonance

    Red lines represent endogenous biological chemiluminescence from germinating A. thaliana seedlings samples. Green line represents signal from agar. The lines are produced from the raw data using smoothed LOESS algorithm. Note that the photodetector noise (mean counts/s = 12.5) is included in the signals.

    Journal: Scientific Reports

    Article Title: Endogenous Chemiluminescence from Germinating Arabidopsis Thaliana Seeds

    doi: 10.1038/s41598-018-34485-6

    Figure Lengend Snippet: Red lines represent endogenous biological chemiluminescence from germinating A. thaliana seedlings samples. Green line represents signal from agar. The lines are produced from the raw data using smoothed LOESS algorithm. Note that the photodetector noise (mean counts/s = 12.5) is included in the signals.

    Article Snippet: For imaging of the A. thaliana seedlings and agar were kept at 30 cm away from the EM-CCD camera.

    Techniques: Produced

    Hydrogen peroxide (H 2 O 2 ) increases intensity of endogenous chemiluminescence of A. thaliana seedlings in a dose-dependent manner. H 2 O 2 was applied on germinating seeds after three days after a deposition on agar plate. There was always 60 s gap between injection of H 2 O 2 and start of the measurement due to sample manipulation. Standard error of mean is calculated for n = 3 measurements.

    Journal: Scientific Reports

    Article Title: Endogenous Chemiluminescence from Germinating Arabidopsis Thaliana Seeds

    doi: 10.1038/s41598-018-34485-6

    Figure Lengend Snippet: Hydrogen peroxide (H 2 O 2 ) increases intensity of endogenous chemiluminescence of A. thaliana seedlings in a dose-dependent manner. H 2 O 2 was applied on germinating seeds after three days after a deposition on agar plate. There was always 60 s gap between injection of H 2 O 2 and start of the measurement due to sample manipulation. Standard error of mean is calculated for n = 3 measurements.

    Article Snippet: For imaging of the A. thaliana seedlings and agar were kept at 30 cm away from the EM-CCD camera.

    Techniques: Injection

    ( a ) Agar dish, ( b ) A. thaliana seeds on agar dish just after deposition, ( c ) A. thaliana seeds germinated on agar dish after three days.

    Journal: Scientific Reports

    Article Title: Endogenous Chemiluminescence from Germinating Arabidopsis Thaliana Seeds

    doi: 10.1038/s41598-018-34485-6

    Figure Lengend Snippet: ( a ) Agar dish, ( b ) A. thaliana seeds on agar dish just after deposition, ( c ) A. thaliana seeds germinated on agar dish after three days.

    Article Snippet: For imaging of the A. thaliana seedlings and agar were kept at 30 cm away from the EM-CCD camera.

    Techniques:

    ( a ) A photograph of the A. thaliana seedlings and agar taken under weak light illumination. ( b ) Endogenous chemiluminescence from A. thaliana seedlings and agar. Imaging was performed using a highly sensitive EM-CCD camera with an integration time of 30 minutes.

    Journal: Scientific Reports

    Article Title: Endogenous Chemiluminescence from Germinating Arabidopsis Thaliana Seeds

    doi: 10.1038/s41598-018-34485-6

    Figure Lengend Snippet: ( a ) A photograph of the A. thaliana seedlings and agar taken under weak light illumination. ( b ) Endogenous chemiluminescence from A. thaliana seedlings and agar. Imaging was performed using a highly sensitive EM-CCD camera with an integration time of 30 minutes.

    Article Snippet: For imaging of the A. thaliana seedlings and agar were kept at 30 cm away from the EM-CCD camera.

    Techniques: Imaging

    Imaging of chemiluminescence signals from A. thaliana seedlings: ( a ) endogenous chemiluminescence with no treatment (control), and after treatment with spraying 250 μ L of ( b ) 1 mM H 2 O 2 , ( c ) 3 mM H 2 O 2 and ( d ) 6 mM H 2 O 2 Imaging was performed using a highly sensitive EM-CCD camera with an integration time of 30 minutes.

    Journal: Scientific Reports

    Article Title: Endogenous Chemiluminescence from Germinating Arabidopsis Thaliana Seeds

    doi: 10.1038/s41598-018-34485-6

    Figure Lengend Snippet: Imaging of chemiluminescence signals from A. thaliana seedlings: ( a ) endogenous chemiluminescence with no treatment (control), and after treatment with spraying 250 μ L of ( b ) 1 mM H 2 O 2 , ( c ) 3 mM H 2 O 2 and ( d ) 6 mM H 2 O 2 Imaging was performed using a highly sensitive EM-CCD camera with an integration time of 30 minutes.

    Article Snippet: For imaging of the A. thaliana seedlings and agar were kept at 30 cm away from the EM-CCD camera.

    Techniques: Imaging

    ( a ) and ( b ) compare the effect of spraying 100 μ L hydrogen peroxide (1 mM) and water (as a control treatment) on the chemiluminescence from A. thaliana seedlings and agar autochemiluminescence, respectively. The smooth solid lines show the best fit of the data using a polynomial function. The chemiluminescence measurement started at 30 minutes after completion of the test on the third day, and it was measured for 30 minutes using a photomultiplier detector. Samples were kept in the dark for three days before treatment and measurement. There was always a 60 s gap between injection of either H 2 O 2 or water and the start of the measurement due to sample manipulation.

    Journal: Scientific Reports

    Article Title: Endogenous Chemiluminescence from Germinating Arabidopsis Thaliana Seeds

    doi: 10.1038/s41598-018-34485-6

    Figure Lengend Snippet: ( a ) and ( b ) compare the effect of spraying 100 μ L hydrogen peroxide (1 mM) and water (as a control treatment) on the chemiluminescence from A. thaliana seedlings and agar autochemiluminescence, respectively. The smooth solid lines show the best fit of the data using a polynomial function. The chemiluminescence measurement started at 30 minutes after completion of the test on the third day, and it was measured for 30 minutes using a photomultiplier detector. Samples were kept in the dark for three days before treatment and measurement. There was always a 60 s gap between injection of either H 2 O 2 or water and the start of the measurement due to sample manipulation.

    Article Snippet: For imaging of the A. thaliana seedlings and agar were kept at 30 cm away from the EM-CCD camera.

    Techniques: Injection

    Biphasic [Ca 2+ ] c transient induced by gravistimulation in Arabidopsis seedlings. A, Typical time course of changes in luminescence ratio induced by gravistimulation (±180° rotation). Approximately 40 Arabidopsis seedlings grown

    Journal: Plant Physiology

    Article Title: Cytoplasmic Calcium Increases in Response to Changes in the Gravity Vector in Hypocotyls and Petioles of Arabidopsis Seedlings 1

    doi: 10.1104/pp.107.106450

    Figure Lengend Snippet: Biphasic [Ca 2+ ] c transient induced by gravistimulation in Arabidopsis seedlings. A, Typical time course of changes in luminescence ratio induced by gravistimulation (±180° rotation). Approximately 40 Arabidopsis seedlings grown

    Article Snippet: Bright-field images of Arabidopsis seedlings were taken with a digital camera (model D70s, Nikon) under approximately 80 μ mol m−2 s−1 white light at the end of each experiment.

    Techniques:

    Schematic diagrams of a device for gravistimulation and a photon-counting system. A, A plate of Arabidopsis seedlings was mounted under an ultrasensitive PCC or a PMT in a light-tight dark box connected to a stepping motor, enabling gravistimulation while

    Journal: Plant Physiology

    Article Title: Cytoplasmic Calcium Increases in Response to Changes in the Gravity Vector in Hypocotyls and Petioles of Arabidopsis Seedlings 1

    doi: 10.1104/pp.107.106450

    Figure Lengend Snippet: Schematic diagrams of a device for gravistimulation and a photon-counting system. A, A plate of Arabidopsis seedlings was mounted under an ultrasensitive PCC or a PMT in a light-tight dark box connected to a stepping motor, enabling gravistimulation while

    Article Snippet: Bright-field images of Arabidopsis seedlings were taken with a digital camera (model D70s, Nikon) under approximately 80 μ mol m−2 s−1 white light at the end of each experiment.

    Techniques: Periodic Counter-current Chromatography

    Comparison of the root hair growth rate and root hair calcium oscillation frequencies present in wild-type Arabidopsis seedling grown in control conditions or in a medium supplemented with 50 nM NAA. Germinated seedlings (36–40 h after vernalization) were transferred from MS/2 standard plates to FEP tubes supplemented or not with 50 nM NAA, and seedlings were visualized after 6 d through the light sheet set-up. (A) Statistical analysis of root hair growth velocity. Values are means ± SD ( n CNT = 43 and n NAA = 31). The two main peaks in the PSD of the control (B) and NAA-treated samples (C) ( n CNT = 43 and n NAA = 31) were plotted and clustered through a k -mean algorithm with k = 2. The low frequency cluster is shown by blue circles, while the high frequency cluster is shown by orange triangles. Black crosses are used to visualize the computed barycenters. The mean and SE of the two main frequencies (LF, low frequency; HF, high frequency) of the two populations is presented in (D).

    Journal: Plant and Cell Physiology

    Article Title: Light Sheet Fluorescence Microscopy Quantifies Calcium Oscillations in Root Hairs of Arabidopsis thaliana

    doi: 10.1093/pcp/pcx045

    Figure Lengend Snippet: Comparison of the root hair growth rate and root hair calcium oscillation frequencies present in wild-type Arabidopsis seedling grown in control conditions or in a medium supplemented with 50 nM NAA. Germinated seedlings (36–40 h after vernalization) were transferred from MS/2 standard plates to FEP tubes supplemented or not with 50 nM NAA, and seedlings were visualized after 6 d through the light sheet set-up. (A) Statistical analysis of root hair growth velocity. Values are means ± SD ( n CNT = 43 and n NAA = 31). The two main peaks in the PSD of the control (B) and NAA-treated samples (C) ( n CNT = 43 and n NAA = 31) were plotted and clustered through a k -mean algorithm with k = 2. The low frequency cluster is shown by blue circles, while the high frequency cluster is shown by orange triangles. Black crosses are used to visualize the computed barycenters. The mean and SE of the two main frequencies (LF, low frequency; HF, high frequency) of the two populations is presented in (D).

    Article Snippet: To carry out the measurement of root and root hair length in Petri dishes, Arabidopsis seedlings were grown on the standard medium jellified with 0.5% Phytagel™ (w/v) (Duchefa) or supplemented with the auxin NAA with a final concentration of 50 nM.

    Techniques: Mass Spectrometry

    Close to physiological mounting procedure for 3D imaging of Arabidopsis seedling roots. After cleaning, FEP tubes were coupled with the upper part of a 10 μl pipette tip and filled with a Phytagel™-based solution (A). A layer of agarose-based solution was then placed on top of the tube (B), where the seedlings were placed (D) after germination in Petri dishes (C). The roots grew inside the jelly matrix (E), and developing root hairs could be imaged thorough the FEP tube. Three-dimensional reconstructions of Arabidopsis root expressing the cytosolic-targeted Cameleon NES-YC3.6. Slices were acquired with a step of 1.5 μm. The single channel reconstruction (F) shows the root hairs three-dimensionally organized around the mature zone. Root hairs look straight, smooth and usually follow gravitropism, as shown in detail in (G). The same consideration can be made by looking at the FRET reconstruction (H). The arrow in (F–H) indicates the shoot position.

    Journal: Plant and Cell Physiology

    Article Title: Light Sheet Fluorescence Microscopy Quantifies Calcium Oscillations in Root Hairs of Arabidopsis thaliana

    doi: 10.1093/pcp/pcx045

    Figure Lengend Snippet: Close to physiological mounting procedure for 3D imaging of Arabidopsis seedling roots. After cleaning, FEP tubes were coupled with the upper part of a 10 μl pipette tip and filled with a Phytagel™-based solution (A). A layer of agarose-based solution was then placed on top of the tube (B), where the seedlings were placed (D) after germination in Petri dishes (C). The roots grew inside the jelly matrix (E), and developing root hairs could be imaged thorough the FEP tube. Three-dimensional reconstructions of Arabidopsis root expressing the cytosolic-targeted Cameleon NES-YC3.6. Slices were acquired with a step of 1.5 μm. The single channel reconstruction (F) shows the root hairs three-dimensionally organized around the mature zone. Root hairs look straight, smooth and usually follow gravitropism, as shown in detail in (G). The same consideration can be made by looking at the FRET reconstruction (H). The arrow in (F–H) indicates the shoot position.

    Article Snippet: To carry out the measurement of root and root hair length in Petri dishes, Arabidopsis seedlings were grown on the standard medium jellified with 0.5% Phytagel™ (w/v) (Duchefa) or supplemented with the auxin NAA with a final concentration of 50 nM.

    Techniques: Imaging, Transferring, Expressing

    Comparison between wild-type Arabidopsis seedling grown in a standard MS/2 medium or supplemented with 50 nM NAA. Primary root (A) and root hair length (B) of seedlings germinated and grown in Petri dishes in the presence or absence of 50 nM NAA. Statistical analysis shows that plants grown in the presence of NAA have, on average, a shorter primary root ( n CNT = 20 and n NAA = 18) (A) and longer root hairs ( n CNT = 23 and n NAA = 29) (B). Values are means ± SD.

    Journal: Plant and Cell Physiology

    Article Title: Light Sheet Fluorescence Microscopy Quantifies Calcium Oscillations in Root Hairs of Arabidopsis thaliana

    doi: 10.1093/pcp/pcx045

    Figure Lengend Snippet: Comparison between wild-type Arabidopsis seedling grown in a standard MS/2 medium or supplemented with 50 nM NAA. Primary root (A) and root hair length (B) of seedlings germinated and grown in Petri dishes in the presence or absence of 50 nM NAA. Statistical analysis shows that plants grown in the presence of NAA have, on average, a shorter primary root ( n CNT = 20 and n NAA = 18) (A) and longer root hairs ( n CNT = 23 and n NAA = 29) (B). Values are means ± SD.

    Article Snippet: To carry out the measurement of root and root hair length in Petri dishes, Arabidopsis seedlings were grown on the standard medium jellified with 0.5% Phytagel™ (w/v) (Duchefa) or supplemented with the auxin NAA with a final concentration of 50 nM.

    Techniques: Mass Spectrometry

    Identification of the NLS in NPR1 by Mutagenesis. (A) Subcellular localization of GFP, NPR1-GFP, and npr1Δ57-GFP (a mutant lacking the C-terminal 57 amino acids of NPR1). GFP fluorescence (top images) and differential interference contrast images (middle images) of onion epidermal cells were compared to show the subcellular localization of GFP (cytoplasmic and nuclear), NPR1-GFP (nuclear), and npr1Δ57-GFP (cytoplasmic). Confocal GFP images (bottom images) were captured from 7-day-old transgenic Arabidopsis seedlings expressing GFP, NPR1-GFP, or npr1Δ57-GFP grown on MS-INA. GFP fluorescence is shown in the green channel; differential interference contrast images are shown in the red channel. (B) Sequence of the C-terminal 57 amino acids of NPR1. The two potential NLSs are underlined. Point mutations in the amino acids shown in red had a marked effect on NPR1-GFP nuclear localization, whereas mutations in the amino acids shown in italics had no detectable effect on nuclear localization. Mutations in all five amino acids shown in red resulted in the exclusive cytoplasmic localization of the fusion protein npr1nls-GFP. (C) Cytoplasmic localization of npr1nls-GFP in leaf epidermal cells of transgenic seedlings.

    Journal: The Plant Cell

    Article Title: Nuclear Localization of NPR1 Is Required for Activation of PR Gene Expression

    doi:

    Figure Lengend Snippet: Identification of the NLS in NPR1 by Mutagenesis. (A) Subcellular localization of GFP, NPR1-GFP, and npr1Δ57-GFP (a mutant lacking the C-terminal 57 amino acids of NPR1). GFP fluorescence (top images) and differential interference contrast images (middle images) of onion epidermal cells were compared to show the subcellular localization of GFP (cytoplasmic and nuclear), NPR1-GFP (nuclear), and npr1Δ57-GFP (cytoplasmic). Confocal GFP images (bottom images) were captured from 7-day-old transgenic Arabidopsis seedlings expressing GFP, NPR1-GFP, or npr1Δ57-GFP grown on MS-INA. GFP fluorescence is shown in the green channel; differential interference contrast images are shown in the red channel. (B) Sequence of the C-terminal 57 amino acids of NPR1. The two potential NLSs are underlined. Point mutations in the amino acids shown in red had a marked effect on NPR1-GFP nuclear localization, whereas mutations in the amino acids shown in italics had no detectable effect on nuclear localization. Mutations in all five amino acids shown in red resulted in the exclusive cytoplasmic localization of the fusion protein npr1nls-GFP. (C) Cytoplasmic localization of npr1nls-GFP in leaf epidermal cells of transgenic seedlings.

    Article Snippet: Arabidopsis seedlings and leaf tissues were mounted in water and viewed with a Zeiss (Jena, Germany) LSM 410 inverted confocal microscope.

    Techniques: Mutagenesis, Fluorescence, Transgenic Assay, Expressing, Mass Spectrometry, Sequencing

    Complementation and Cosuppression Effect of the 35S : FLAG-CAND1 Transgene. (A) Expression of the FLAG-CAND1 fusion protein. Flower protein extracts from wild-type Arabidopsis, cand1-1 mutant, and various 35S : FLAG-CAND1 transgenic Arabidopsis lines (line numbers labeled at the top) were subjected to immunoblot analysis with anti-CAND1 and anti-RPN6 antibodies. Lines marked as +/− are heterozygous for cand1-1 mutation, and lines marked as −/− are homozygous for the cand1-1 mutation. Arrowheads indicate protein positions. The asterisk marks a cross-reacting band. RPN6 is used as a loading control. (B) Comparison of partial complementation phenotypes and cosuppression phenotypes. An arrow is put at the top of each line with the name as labeled above. Plants shown next to the indicated lines are other transgenic Arabidopsis lines that are heterozygous for the cand1-1 mutation. Pictures were taken when the plants were 7 weeks old.

    Journal: The Plant Cell

    Article Title: Arabidopsis CAND1, an Unmodified CUL1-Interacting Protein, Is Involved in Multiple Developmental Pathways Controlled by Ubiquitin/Proteasome-Mediated Protein Degradation

    doi: 10.1105/tpc.021949

    Figure Lengend Snippet: Complementation and Cosuppression Effect of the 35S : FLAG-CAND1 Transgene. (A) Expression of the FLAG-CAND1 fusion protein. Flower protein extracts from wild-type Arabidopsis, cand1-1 mutant, and various 35S : FLAG-CAND1 transgenic Arabidopsis lines (line numbers labeled at the top) were subjected to immunoblot analysis with anti-CAND1 and anti-RPN6 antibodies. Lines marked as +/− are heterozygous for cand1-1 mutation, and lines marked as −/− are homozygous for the cand1-1 mutation. Arrowheads indicate protein positions. The asterisk marks a cross-reacting band. RPN6 is used as a loading control. (B) Comparison of partial complementation phenotypes and cosuppression phenotypes. An arrow is put at the top of each line with the name as labeled above. Plants shown next to the indicated lines are other transgenic Arabidopsis lines that are heterozygous for the cand1-1 mutation. Pictures were taken when the plants were 7 weeks old.

    Article Snippet: To grow Arabidopsis seedlings, seeds were surface sterilized, put on MS plates (Gibco, Cleveland, OH) containing 1% sucrose, and cold treated at 4°C for 3 to 5 d before being placed in a standard, continuous white light growth chamber or in complete darkness at 22°C.

    Techniques: Expressing, Mutagenesis, Transgenic Assay, Labeling

    CAND1 Functions in Photomorphogenesis by Regulating HY5 Degradation. (A) and (B) Loss of CAND1 leads to constitutive photomorphogenesis in dark and enhances phenotypes of weak cop alleles. Different Arabidopsis lines (labeled at the top) were grown in complete darkness for the indicated number of days. All pictures were taken under the same magnification. (C) The cand1-1 mutation causes hyperaccumulation of HY5 in dark-grown seedlings. Seedling protein extracts from 4-d-old light-grown wild-type Arabidopsis, dark-grown wild-type Arabidopsis, and various dark-grown single mutants and double mutants (labeled at the top) were prepared and blotted by anti-HY5 and anti-RPN6 antibodies. (D) HY5 is degraded less efficiently in the cand1-1 mutant than in wild-type Arabidopsis. Four-day-old light-grown seedlings of wild-type Arabidopsis and cand1-1 mutant were transferred to complete darkness. Samples were collected at different time points starting from the transfer (indicated at the top) and blotted with anti-HY5 and anti-RPN6 antibodies. The arrowheads and asterisk in (C) and (D) indicate protein positions and a cross-reacting band, respectively. HY5 has two forms: unphosphorylated (bottom band) and phosphorylated (top band). RPN6 is used as a loading control.

    Journal: The Plant Cell

    Article Title: Arabidopsis CAND1, an Unmodified CUL1-Interacting Protein, Is Involved in Multiple Developmental Pathways Controlled by Ubiquitin/Proteasome-Mediated Protein Degradation

    doi: 10.1105/tpc.021949

    Figure Lengend Snippet: CAND1 Functions in Photomorphogenesis by Regulating HY5 Degradation. (A) and (B) Loss of CAND1 leads to constitutive photomorphogenesis in dark and enhances phenotypes of weak cop alleles. Different Arabidopsis lines (labeled at the top) were grown in complete darkness for the indicated number of days. All pictures were taken under the same magnification. (C) The cand1-1 mutation causes hyperaccumulation of HY5 in dark-grown seedlings. Seedling protein extracts from 4-d-old light-grown wild-type Arabidopsis, dark-grown wild-type Arabidopsis, and various dark-grown single mutants and double mutants (labeled at the top) were prepared and blotted by anti-HY5 and anti-RPN6 antibodies. (D) HY5 is degraded less efficiently in the cand1-1 mutant than in wild-type Arabidopsis. Four-day-old light-grown seedlings of wild-type Arabidopsis and cand1-1 mutant were transferred to complete darkness. Samples were collected at different time points starting from the transfer (indicated at the top) and blotted with anti-HY5 and anti-RPN6 antibodies. The arrowheads and asterisk in (C) and (D) indicate protein positions and a cross-reacting band, respectively. HY5 has two forms: unphosphorylated (bottom band) and phosphorylated (top band). RPN6 is used as a loading control.

    Article Snippet: To grow Arabidopsis seedlings, seeds were surface sterilized, put on MS plates (Gibco, Cleveland, OH) containing 1% sucrose, and cold treated at 4°C for 3 to 5 d before being placed in a standard, continuous white light growth chamber or in complete darkness at 22°C.

    Techniques: Labeling, Mutagenesis

    Jasmonate and Auxin Responses Are Affected in the cand1-1 Mutant. The root growth inhibitions conferred by jasmonate (A) and auxin (B) are reduced in the cand1-1 mutant. Arabidopsis lines used in the experiments are indicated at the right. Concentrations of hormones used in MS medium are indicated at the bottom. Root length on MS medium without jasmonates or auxin was set as 100%, which did not show any significant difference among the wild type and various mutant lines. Error bars represent standard deviation ( n > 10). MeJA, methyl jasmonate.

    Journal: The Plant Cell

    Article Title: Arabidopsis CAND1, an Unmodified CUL1-Interacting Protein, Is Involved in Multiple Developmental Pathways Controlled by Ubiquitin/Proteasome-Mediated Protein Degradation

    doi: 10.1105/tpc.021949

    Figure Lengend Snippet: Jasmonate and Auxin Responses Are Affected in the cand1-1 Mutant. The root growth inhibitions conferred by jasmonate (A) and auxin (B) are reduced in the cand1-1 mutant. Arabidopsis lines used in the experiments are indicated at the right. Concentrations of hormones used in MS medium are indicated at the bottom. Root length on MS medium without jasmonates or auxin was set as 100%, which did not show any significant difference among the wild type and various mutant lines. Error bars represent standard deviation ( n > 10). MeJA, methyl jasmonate.

    Article Snippet: To grow Arabidopsis seedlings, seeds were surface sterilized, put on MS plates (Gibco, Cleveland, OH) containing 1% sucrose, and cold treated at 4°C for 3 to 5 d before being placed in a standard, continuous white light growth chamber or in complete darkness at 22°C.

    Techniques: Mutagenesis, Mass Spectrometry, Standard Deviation

    CAND1 Selectively Interacts with Unmodified CUL1 in Vivo. (A) Unmodified CUL1 was precipitated together with FLAG-CAND1. Seedling protein extracts prepared from wild-type Arabidopsis and 35S : FLAG-CAND1 transgenic Arabidopsis (line 6-7) were incubated with anti-FLAG antibody conjugated agarose (α-FLAG). The precipitates and the total extracts were subjected to immunoblot analysis with antibodies against FLAG, CUL1, and TATA binding protein (TBP). (B) and (C) CAND1 associates with CUL1-TAP but not with TAP-CUL1 or ASK1-TAP in vivo. Total flower protein extracts prepared from wild-type Arabidopsis, 35S : CUL1-TAP , 35S : TAP-CUL1 , and 35S : ASK1-TAP transgenic Arabidopsis were incubated with IgG-coupled sepharose. The precipitates and the total extracts were subjected to immunoblot analysis with antibodies against CAND1, TATA binding protein (TBP), and CUL1 (B) or MYC (C) . In (A) to (C) , arrowheads indicate protein positions, and T indicates total protein extract. Anti-TBP (TATA binding protein) antibody is used as a pull-down control. (D) CAND1 interacts with RUB modification site mutated CUL1 more strongly than with wild-type CUL1 in yeast two-hybrid assays. β-gal activity resulted from CUL1(K682R) and CAND1 interaction is set to 100%. Error bars represent standard deviation ( n = 4). (E) Arabidopsis CUL1 is modified by RUB in yeast, which can be abolished by a point mutation at its RUB modification site. Yeast strains used in two-hybrid assays (labeled at the top) were subjected to immunoblot analysis with antibodies against CUL1 and CAND1. Arrowheads indicate protein positions. The asterisk marks a nonspecific band cross-reacting with anti-CUL1 antibodies, which is also used as a loading control of the total protein amount in each lane.

    Journal: The Plant Cell

    Article Title: Arabidopsis CAND1, an Unmodified CUL1-Interacting Protein, Is Involved in Multiple Developmental Pathways Controlled by Ubiquitin/Proteasome-Mediated Protein Degradation

    doi: 10.1105/tpc.021949

    Figure Lengend Snippet: CAND1 Selectively Interacts with Unmodified CUL1 in Vivo. (A) Unmodified CUL1 was precipitated together with FLAG-CAND1. Seedling protein extracts prepared from wild-type Arabidopsis and 35S : FLAG-CAND1 transgenic Arabidopsis (line 6-7) were incubated with anti-FLAG antibody conjugated agarose (α-FLAG). The precipitates and the total extracts were subjected to immunoblot analysis with antibodies against FLAG, CUL1, and TATA binding protein (TBP). (B) and (C) CAND1 associates with CUL1-TAP but not with TAP-CUL1 or ASK1-TAP in vivo. Total flower protein extracts prepared from wild-type Arabidopsis, 35S : CUL1-TAP , 35S : TAP-CUL1 , and 35S : ASK1-TAP transgenic Arabidopsis were incubated with IgG-coupled sepharose. The precipitates and the total extracts were subjected to immunoblot analysis with antibodies against CAND1, TATA binding protein (TBP), and CUL1 (B) or MYC (C) . In (A) to (C) , arrowheads indicate protein positions, and T indicates total protein extract. Anti-TBP (TATA binding protein) antibody is used as a pull-down control. (D) CAND1 interacts with RUB modification site mutated CUL1 more strongly than with wild-type CUL1 in yeast two-hybrid assays. β-gal activity resulted from CUL1(K682R) and CAND1 interaction is set to 100%. Error bars represent standard deviation ( n = 4). (E) Arabidopsis CUL1 is modified by RUB in yeast, which can be abolished by a point mutation at its RUB modification site. Yeast strains used in two-hybrid assays (labeled at the top) were subjected to immunoblot analysis with antibodies against CUL1 and CAND1. Arrowheads indicate protein positions. The asterisk marks a nonspecific band cross-reacting with anti-CUL1 antibodies, which is also used as a loading control of the total protein amount in each lane.

    Article Snippet: To grow Arabidopsis seedlings, seeds were surface sterilized, put on MS plates (Gibco, Cleveland, OH) containing 1% sucrose, and cold treated at 4°C for 3 to 5 d before being placed in a standard, continuous white light growth chamber or in complete darkness at 22°C.

    Techniques: In Vivo, Transgenic Assay, Incubation, Binding Assay, Modification, Activity Assay, Standard Deviation, Mutagenesis, Labeling

    Abnormal Development of Shoot Apex and Flower in the cand1-1 Mutant. (A) Flowering time of wild-type Arabidopsis and cand1-1 mutant, as indicated by average number of rosette leaves at the time of bolting. The identities of the plants are labeled at the bottom. Error bars represent standard deviation ( n = 6). (B) to (E) Aerial rosettes are formed in the axils of the cand1-1 mutant. Samples were taken from different parts of the same plant, including the bottom (B) , the middle (C) , and the tip of the axillary branch ( [D] and [E] ). (F) Comparison of wild-type and cand1-1 flowers. The flowers were dissected to reveal the floral organs. Pictures were taken under the same magnification. (G) Comparison of wild-type and cand1-1 siliques. (H) UFO-MYC protein level is greatly reduced in the cand1-1 mutant background. Protein samples were extracted from flowers of wild-type Arabidopsis, 35S : UFO-MYC transgenic Arabidopsis ( UM ), cand1-1 mutant expressing 35S : FLAG-CAND1 transgene ( UM/cand1-1 ), and cand1-1 mutant. The extracts were then subjected to immunoblot analysis with anti-CAND1, anti-MYC, and anti-RPN6 antibodies. Arrowheads indicate protein positions. The asterisks mark two cross-reacting bands. RPN6 is used as a loading control. (I) UFO overexpression phenotypes are overridden by cand1-1 mutation. The identities of the flowers are indicated at the bottom. The flowers were dissected to reveal the floral organs.

    Journal: The Plant Cell

    Article Title: Arabidopsis CAND1, an Unmodified CUL1-Interacting Protein, Is Involved in Multiple Developmental Pathways Controlled by Ubiquitin/Proteasome-Mediated Protein Degradation

    doi: 10.1105/tpc.021949

    Figure Lengend Snippet: Abnormal Development of Shoot Apex and Flower in the cand1-1 Mutant. (A) Flowering time of wild-type Arabidopsis and cand1-1 mutant, as indicated by average number of rosette leaves at the time of bolting. The identities of the plants are labeled at the bottom. Error bars represent standard deviation ( n = 6). (B) to (E) Aerial rosettes are formed in the axils of the cand1-1 mutant. Samples were taken from different parts of the same plant, including the bottom (B) , the middle (C) , and the tip of the axillary branch ( [D] and [E] ). (F) Comparison of wild-type and cand1-1 flowers. The flowers were dissected to reveal the floral organs. Pictures were taken under the same magnification. (G) Comparison of wild-type and cand1-1 siliques. (H) UFO-MYC protein level is greatly reduced in the cand1-1 mutant background. Protein samples were extracted from flowers of wild-type Arabidopsis, 35S : UFO-MYC transgenic Arabidopsis ( UM ), cand1-1 mutant expressing 35S : FLAG-CAND1 transgene ( UM/cand1-1 ), and cand1-1 mutant. The extracts were then subjected to immunoblot analysis with anti-CAND1, anti-MYC, and anti-RPN6 antibodies. Arrowheads indicate protein positions. The asterisks mark two cross-reacting bands. RPN6 is used as a loading control. (I) UFO overexpression phenotypes are overridden by cand1-1 mutation. The identities of the flowers are indicated at the bottom. The flowers were dissected to reveal the floral organs.

    Article Snippet: To grow Arabidopsis seedlings, seeds were surface sterilized, put on MS plates (Gibco, Cleveland, OH) containing 1% sucrose, and cold treated at 4°C for 3 to 5 d before being placed in a standard, continuous white light growth chamber or in complete darkness at 22°C.

    Techniques: Mutagenesis, Labeling, Standard Deviation, Transgenic Assay, Expressing, Over Expression

    Identification and Characterization of Arabidopsis CAND1 Gene and Its T-DNA Insertional Mutants. (A) A phylogenetic tree of CAND1 proteins from representative eukaryotic organisms as labeled at the right. (B) Schematic diagram of T-DNA insertions in the Arabidopsis CAND1 gene (At2g02560). Exons are represented by closed (coding region) and open (untranslated regions) boxes, whereas introns are represented by lines. The T-DNA insertion sites of the three mutant alleles are indicated by arrows, with the assigned allele names for each insertional mutation at the top. (C) The cand1 mutations abolish CAND1 expression but do not affect CUL1 accumulation. Flower protein extracts were prepared from wild-type Arabidopsis and three cand1 mutants and subjected to immunoblot analysis with anti-CAND1, anti-CUL1, and anti-RPN6 antibodies. Arrowheads indicate protein positions. RPN6 is used as a loading control. (D) All three cand1 mutants show similar phenotypes when grown under identical conditions. Numbers at the right of each row indicate the age of the plants when photographed.

    Journal: The Plant Cell

    Article Title: Arabidopsis CAND1, an Unmodified CUL1-Interacting Protein, Is Involved in Multiple Developmental Pathways Controlled by Ubiquitin/Proteasome-Mediated Protein Degradation

    doi: 10.1105/tpc.021949

    Figure Lengend Snippet: Identification and Characterization of Arabidopsis CAND1 Gene and Its T-DNA Insertional Mutants. (A) A phylogenetic tree of CAND1 proteins from representative eukaryotic organisms as labeled at the right. (B) Schematic diagram of T-DNA insertions in the Arabidopsis CAND1 gene (At2g02560). Exons are represented by closed (coding region) and open (untranslated regions) boxes, whereas introns are represented by lines. The T-DNA insertion sites of the three mutant alleles are indicated by arrows, with the assigned allele names for each insertional mutation at the top. (C) The cand1 mutations abolish CAND1 expression but do not affect CUL1 accumulation. Flower protein extracts were prepared from wild-type Arabidopsis and three cand1 mutants and subjected to immunoblot analysis with anti-CAND1, anti-CUL1, and anti-RPN6 antibodies. Arrowheads indicate protein positions. RPN6 is used as a loading control. (D) All three cand1 mutants show similar phenotypes when grown under identical conditions. Numbers at the right of each row indicate the age of the plants when photographed.

    Article Snippet: To grow Arabidopsis seedlings, seeds were surface sterilized, put on MS plates (Gibco, Cleveland, OH) containing 1% sucrose, and cold treated at 4°C for 3 to 5 d before being placed in a standard, continuous white light growth chamber or in complete darkness at 22°C.

    Techniques: Labeling, Mutagenesis, Expressing

    Proper GA Signaling Requires CAND1. (A) The GA pathway negative regulator RGA accumulates in the cand1-1 mutant. Flower protein extracts from wild-type Arabidopsis, cand1-1 , rga-24 (negative control), and sly1-10 mutants were subjected to immunoblot analysis with anti-RGA and anti-RPN6 antibodies. (B) The cand1-1 mutant fails to rapidly destabilize RGA upon GA treatment. Eight-day-old wild-type and cand1-1 seedlings were treated with GA 3 for 2 h. Subsequently, protein extracts were prepared from treated (+) and untreated (−) seedlings and subjected to immunoblot analysis with anti-RGA and anti-RPN6 antibodies. The arrowheads in (A) and (B) indicate protein positions. RPN6 is used as a loading control.

    Journal: The Plant Cell

    Article Title: Arabidopsis CAND1, an Unmodified CUL1-Interacting Protein, Is Involved in Multiple Developmental Pathways Controlled by Ubiquitin/Proteasome-Mediated Protein Degradation

    doi: 10.1105/tpc.021949

    Figure Lengend Snippet: Proper GA Signaling Requires CAND1. (A) The GA pathway negative regulator RGA accumulates in the cand1-1 mutant. Flower protein extracts from wild-type Arabidopsis, cand1-1 , rga-24 (negative control), and sly1-10 mutants were subjected to immunoblot analysis with anti-RGA and anti-RPN6 antibodies. (B) The cand1-1 mutant fails to rapidly destabilize RGA upon GA treatment. Eight-day-old wild-type and cand1-1 seedlings were treated with GA 3 for 2 h. Subsequently, protein extracts were prepared from treated (+) and untreated (−) seedlings and subjected to immunoblot analysis with anti-RGA and anti-RPN6 antibodies. The arrowheads in (A) and (B) indicate protein positions. RPN6 is used as a loading control.

    Article Snippet: To grow Arabidopsis seedlings, seeds were surface sterilized, put on MS plates (Gibco, Cleveland, OH) containing 1% sucrose, and cold treated at 4°C for 3 to 5 d before being placed in a standard, continuous white light growth chamber or in complete darkness at 22°C.

    Techniques: Mutagenesis, Negative Control

    Constitutively expressing TaBASS2 represses ABI4 expression. a , b The expression levels of ABI4 and HKT1;1 in 12-day-old wild-type and two 35S::TaBASS2 transgenic lines (OE1 and OE3). Error bars represent the standard errors ( n = 3), with each replicate comprising at least 12 plants. The expression levels were determined by RT-qPCR using AtACT2 in Arabidopsis as the internal control c , d The Na + contents in the shoot and root tissue from ten-day-old wild-type and OE lines. Error bars represent the standard errors ( n = 3), with each replicate comprising at least 200 plants. Columns labeled with an asterisk indicate means differing significantly from the WT result ( P

    Journal: BMC Plant Biology

    Article Title: A putative pyruvate transporter TaBASS2 positively regulates salinity tolerance in wheat via modulation of ABI4 expression

    doi: 10.1186/s12870-016-0795-3

    Figure Lengend Snippet: Constitutively expressing TaBASS2 represses ABI4 expression. a , b The expression levels of ABI4 and HKT1;1 in 12-day-old wild-type and two 35S::TaBASS2 transgenic lines (OE1 and OE3). Error bars represent the standard errors ( n = 3), with each replicate comprising at least 12 plants. The expression levels were determined by RT-qPCR using AtACT2 in Arabidopsis as the internal control c , d The Na + contents in the shoot and root tissue from ten-day-old wild-type and OE lines. Error bars represent the standard errors ( n = 3), with each replicate comprising at least 200 plants. Columns labeled with an asterisk indicate means differing significantly from the WT result ( P

    Article Snippet: Catalase activities were measured in ten-day-old Arabidopsis seedlings with a commercial kit purchased from Beyotime Institute of Biotechnology (Haimen, China).

    Techniques: Expressing, Transgenic Assay, Quantitative RT-PCR, Labeling

    The enhanced salinity tolerance in 35S::TaBASS2 Arabidopsis plants relies on ABI4 suppression. a - d The wild-type seedlings and two transgenic lines constitutively expressing both TaBASS2 and ABI4 (TaBASS2OE ABI4OE #17 and #19) after a ten-day treatment with 0, 50, 100 or 125 mM NaCl. Bar = 1 cm. e Relative root growth of the wild-type and TaBASS2OE ABI4OE seedlings. Error bars represent the standard errors ( n = 3), with each replicate comprising at least 30 seedlings. f , g The expression levels of ABI4 ( f ) and HKT1;1 ( g ) in the wild-type and TaBASS2OE ABI4OE seedlings. Error bars represent the standard errors ( n = 3), with each replicate comprising at least 12 seedlings. The expression levels were determined by RT-qPCR using AtACT2 in Arabidopsis as the internal control

    Journal: BMC Plant Biology

    Article Title: A putative pyruvate transporter TaBASS2 positively regulates salinity tolerance in wheat via modulation of ABI4 expression

    doi: 10.1186/s12870-016-0795-3

    Figure Lengend Snippet: The enhanced salinity tolerance in 35S::TaBASS2 Arabidopsis plants relies on ABI4 suppression. a - d The wild-type seedlings and two transgenic lines constitutively expressing both TaBASS2 and ABI4 (TaBASS2OE ABI4OE #17 and #19) after a ten-day treatment with 0, 50, 100 or 125 mM NaCl. Bar = 1 cm. e Relative root growth of the wild-type and TaBASS2OE ABI4OE seedlings. Error bars represent the standard errors ( n = 3), with each replicate comprising at least 30 seedlings. f , g The expression levels of ABI4 ( f ) and HKT1;1 ( g ) in the wild-type and TaBASS2OE ABI4OE seedlings. Error bars represent the standard errors ( n = 3), with each replicate comprising at least 12 seedlings. The expression levels were determined by RT-qPCR using AtACT2 in Arabidopsis as the internal control

    Article Snippet: Catalase activities were measured in ten-day-old Arabidopsis seedlings with a commercial kit purchased from Beyotime Institute of Biotechnology (Haimen, China).

    Techniques: Transgenic Assay, Expressing, Quantitative RT-PCR

    Constitutively expressing TaBASS2 enhances the salinity tolerance in Arabidopsis. a - d The wild-type seedlings and two 35S::TaBASS2 transgenic lines (OE1 and OE3) after a ten-day treatment with 0, 50, 100 or 125 mM NaCl. Bar = 1 cm. e Relative root growth of the wild-type and OE plants treated with 0, 50, 100 or 125 mM NaCl. f The four-week-old soil-grown wild-type and OE plants 0, 10 and 14 days after NaCl treatment. g Plant survival rates measured at 14 day after NaCl treatment. Error bars in ( e , g ) represent the standard errors ( n = 3), with each replicate comprising at least 30 plants. Columns labeled with an asterisk indicate means differing significantly from the WT result ( P

    Journal: BMC Plant Biology

    Article Title: A putative pyruvate transporter TaBASS2 positively regulates salinity tolerance in wheat via modulation of ABI4 expression

    doi: 10.1186/s12870-016-0795-3

    Figure Lengend Snippet: Constitutively expressing TaBASS2 enhances the salinity tolerance in Arabidopsis. a - d The wild-type seedlings and two 35S::TaBASS2 transgenic lines (OE1 and OE3) after a ten-day treatment with 0, 50, 100 or 125 mM NaCl. Bar = 1 cm. e Relative root growth of the wild-type and OE plants treated with 0, 50, 100 or 125 mM NaCl. f The four-week-old soil-grown wild-type and OE plants 0, 10 and 14 days after NaCl treatment. g Plant survival rates measured at 14 day after NaCl treatment. Error bars in ( e , g ) represent the standard errors ( n = 3), with each replicate comprising at least 30 plants. Columns labeled with an asterisk indicate means differing significantly from the WT result ( P

    Article Snippet: Catalase activities were measured in ten-day-old Arabidopsis seedlings with a commercial kit purchased from Beyotime Institute of Biotechnology (Haimen, China).

    Techniques: Expressing, Transgenic Assay, Labeling

    Relative abundance of ACS and ACO multigene family. Relative abundance of ACS (A-B) and ACO (C-D) multigene family members in roots and leaves of 3 weeks old Arabidopsis thaliana plants exposed for 0, 24 or 72 h to either 5 or 10 μM CdSO 4 or grown under control conditions in a hydroponic culture system. Data represent mean abundance of at least 4 biological replicates relative to the control (0 h, 0 μM CdSO 4 ) and with the abundance of the most highly expressed family member set at 1 under the control condition. (A) Relative abundance of ACS multigene family members in roots. (B) Relative abundance of ACS multigene family members in leaves. (C) Relative abundance of ACO multigene family members in roots. (D) Relative abundance of ACO multigene family members in leaves.

    Journal: BMC Plant Biology

    Article Title: Cadmium-induced ethylene production and responses in Arabidopsis thaliana rely on ACS2 and ACS6 gene expression

    doi: 10.1186/s12870-014-0214-6

    Figure Lengend Snippet: Relative abundance of ACS and ACO multigene family. Relative abundance of ACS (A-B) and ACO (C-D) multigene family members in roots and leaves of 3 weeks old Arabidopsis thaliana plants exposed for 0, 24 or 72 h to either 5 or 10 μM CdSO 4 or grown under control conditions in a hydroponic culture system. Data represent mean abundance of at least 4 biological replicates relative to the control (0 h, 0 μM CdSO 4 ) and with the abundance of the most highly expressed family member set at 1 under the control condition. (A) Relative abundance of ACS multigene family members in roots. (B) Relative abundance of ACS multigene family members in leaves. (C) Relative abundance of ACO multigene family members in roots. (D) Relative abundance of ACO multigene family members in leaves.

    Article Snippet: These results correspond to those of Montero-Palmero et al. [ ], who also observed a transient induction of ethylene responses in mercury (Hg) treated Medicago sativa and Arabidopsis thaliana seedlings.

    Techniques:

    Relative expression of ethylene responsive genes. A comparison of the relative expression of ACO2 (A-B) , ETR2 (C-D) and ERF1 (E-F) in roots and leaves of 3 weeks old wild-type or acs2-1acs6-1 mutant Arabidopsis thaliana plants exposed for 24 or 72 h to either 5 or 10 μM CdSO 4 or grown under control conditions in a hydroponic culture system. Data shows mean ± s.e. of at least 4 biological replicates relative to the control (24 h, 0 μM CdSO 4 ). The letters a-d represent groups with a significantly different gene expression (Tukey’s test: p

    Journal: BMC Plant Biology

    Article Title: Cadmium-induced ethylene production and responses in Arabidopsis thaliana rely on ACS2 and ACS6 gene expression

    doi: 10.1186/s12870-014-0214-6

    Figure Lengend Snippet: Relative expression of ethylene responsive genes. A comparison of the relative expression of ACO2 (A-B) , ETR2 (C-D) and ERF1 (E-F) in roots and leaves of 3 weeks old wild-type or acs2-1acs6-1 mutant Arabidopsis thaliana plants exposed for 24 or 72 h to either 5 or 10 μM CdSO 4 or grown under control conditions in a hydroponic culture system. Data shows mean ± s.e. of at least 4 biological replicates relative to the control (24 h, 0 μM CdSO 4 ). The letters a-d represent groups with a significantly different gene expression (Tukey’s test: p

    Article Snippet: These results correspond to those of Montero-Palmero et al. [ ], who also observed a transient induction of ethylene responses in mercury (Hg) treated Medicago sativa and Arabidopsis thaliana seedlings.

    Techniques: Expressing, Mutagenesis

    ACC content. ACC content (free and conjugated; pmol mg −1 FW −1 ) in roots (A) and leaves (B) of 3 weeks old Arabidopsis thaliana plants exposed for 24 or 72 h to either 5 or 10 μM CdSO 4 or grown under control conditions in a hydroponic culture system. Data are given as mean ± s.e. of at least 5 biological replicates. The letters a-d (A) a-c (B) represent groups with significantly different amounts of ACC (Tukey’s test: p

    Journal: BMC Plant Biology

    Article Title: Cadmium-induced ethylene production and responses in Arabidopsis thaliana rely on ACS2 and ACS6 gene expression

    doi: 10.1186/s12870-014-0214-6

    Figure Lengend Snippet: ACC content. ACC content (free and conjugated; pmol mg −1 FW −1 ) in roots (A) and leaves (B) of 3 weeks old Arabidopsis thaliana plants exposed for 24 or 72 h to either 5 or 10 μM CdSO 4 or grown under control conditions in a hydroponic culture system. Data are given as mean ± s.e. of at least 5 biological replicates. The letters a-d (A) a-c (B) represent groups with significantly different amounts of ACC (Tukey’s test: p

    Article Snippet: These results correspond to those of Montero-Palmero et al. [ ], who also observed a transient induction of ethylene responses in mercury (Hg) treated Medicago sativa and Arabidopsis thaliana seedlings.

    Techniques:

    Ethylene emission. A comparison of the ethylene emission (pL mg −1 FW −1 h −1 ) in 3 weeks old wild-type or acs2-1acs6-1 mutant Arabidopsis thaliana plants exposed for 24 or 72 h to 10, 25 or 100 μM CdSO 4 or grown under control conditions in a rockwool culture system. Data are shown as mean ± s.e. of at least 3 biological replicates. The letters a-b represent groups with a significantly different ethylene production (Tukey’s test: p

    Journal: BMC Plant Biology

    Article Title: Cadmium-induced ethylene production and responses in Arabidopsis thaliana rely on ACS2 and ACS6 gene expression

    doi: 10.1186/s12870-014-0214-6

    Figure Lengend Snippet: Ethylene emission. A comparison of the ethylene emission (pL mg −1 FW −1 h −1 ) in 3 weeks old wild-type or acs2-1acs6-1 mutant Arabidopsis thaliana plants exposed for 24 or 72 h to 10, 25 or 100 μM CdSO 4 or grown under control conditions in a rockwool culture system. Data are shown as mean ± s.e. of at least 3 biological replicates. The letters a-b represent groups with a significantly different ethylene production (Tukey’s test: p

    Article Snippet: These results correspond to those of Montero-Palmero et al. [ ], who also observed a transient induction of ethylene responses in mercury (Hg) treated Medicago sativa and Arabidopsis thaliana seedlings.

    Techniques: Mutagenesis

    Biomass growth inhibition. A comparison of the fresh weight biomass and growth inhibition (mg) of roots (A) and leaves (B) of 3 weeks old wild-type or acs2-1acs6-1 mutant Arabidopsis thaliana plants exposed for 24 or 72 h to either 5 or 10 μM CdSO 4 or grown under control conditions in a hydroponic culture system. Biomass: Data shows mean ± s.e. of at least 4 biological replicates. The letters a-c represent groups with a significantly different biomass (Tukey’s test: p

    Journal: BMC Plant Biology

    Article Title: Cadmium-induced ethylene production and responses in Arabidopsis thaliana rely on ACS2 and ACS6 gene expression

    doi: 10.1186/s12870-014-0214-6

    Figure Lengend Snippet: Biomass growth inhibition. A comparison of the fresh weight biomass and growth inhibition (mg) of roots (A) and leaves (B) of 3 weeks old wild-type or acs2-1acs6-1 mutant Arabidopsis thaliana plants exposed for 24 or 72 h to either 5 or 10 μM CdSO 4 or grown under control conditions in a hydroponic culture system. Biomass: Data shows mean ± s.e. of at least 4 biological replicates. The letters a-c represent groups with a significantly different biomass (Tukey’s test: p

    Article Snippet: These results correspond to those of Montero-Palmero et al. [ ], who also observed a transient induction of ethylene responses in mercury (Hg) treated Medicago sativa and Arabidopsis thaliana seedlings.

    Techniques: Inhibition, Mutagenesis

    Characterization of the Arabidopsis sig6 - 2 mutant. A, Genomic Sig6 region (At2g36990) showing exon/intron structure and T-DNA insertion site in exon 5, 1,022 nucleotides downstream from the ATG start codon (top line). The resulting cDNA with the fused exons, but without the 5′ and 3′ untranslated regions, is depicted below. Also given are features of the derived protein. TP, Transit peptide; UR, unconserved region; 1–4, conserved regions for sigma activity. Genomic sequence and cDNA, but not T-DNA, are drawn to scale (scale bar on top). LB, Left border; RB, right border; sul , sulfonamide (sulfadiazine) resistance gene. B, RT-PCR detection of sigma factor transcripts in wild type (WT) and sig6 - 2 mutant. Total RNA was prepared from 6-d seedlings, reverse transcribed, and cDNA was amplified using the gene-specific primer pairs as described in “Materials and Methods.” C, Wild-type (WT) and sig6 - 2 mutant phenotype during development (4, 8, 10, 12, 21, and 28 d after sowing).

    Journal: Plant Physiology

    Article Title: Dual Temporal Role of Plastid Sigma Factor 6 in Arabidopsis Development 1Dual Temporal Role of Plastid Sigma Factor 6 in Arabidopsis Development 1 [OA]

    doi: 10.1104/pp.106.085878

    Figure Lengend Snippet: Characterization of the Arabidopsis sig6 - 2 mutant. A, Genomic Sig6 region (At2g36990) showing exon/intron structure and T-DNA insertion site in exon 5, 1,022 nucleotides downstream from the ATG start codon (top line). The resulting cDNA with the fused exons, but without the 5′ and 3′ untranslated regions, is depicted below. Also given are features of the derived protein. TP, Transit peptide; UR, unconserved region; 1–4, conserved regions for sigma activity. Genomic sequence and cDNA, but not T-DNA, are drawn to scale (scale bar on top). LB, Left border; RB, right border; sul , sulfonamide (sulfadiazine) resistance gene. B, RT-PCR detection of sigma factor transcripts in wild type (WT) and sig6 - 2 mutant. Total RNA was prepared from 6-d seedlings, reverse transcribed, and cDNA was amplified using the gene-specific primer pairs as described in “Materials and Methods.” C, Wild-type (WT) and sig6 - 2 mutant phenotype during development (4, 8, 10, 12, 21, and 28 d after sowing).

    Article Snippet: Total RNA (2 μ g) from 6-d-old Arabidopsis seedlings was mixed with random primers (10 pM; Promega), incubated at 70°C for 10 min, and chilled on ice for 1 min. After addition of 6 μ L avian myeloblastosis virus-reverse transcriptase buffer (Promega), 1 μ L RNasin (40 units/ μ L; Promega), 3 μ L dNTPs (0.25 m m each), and 3 μ L avian myeloblastosis virus-reverse transcriptase (10 units/ μ L) to a final volume of 30 μ L, the reaction was incubated at 37°C for 90 min.

    Techniques: Mutagenesis, Derivative Assay, Activity Assay, Sequencing, Reverse Transcription Polymerase Chain Reaction, Amplification

    Analysis of the Expression of the Arabidopsis BIO3-BIO1 Locus at the Protein Level and Subcellular Distribution. (A) Immunological detection of BIO3-BIO1 gene products in Arabidopsis . Soluble proteins (40 µg) from aboveground organs from Arabidopsis plants (lane 1) and Arabidopsis cultured cells (lane 2) were separated by SDS-PAGE and analyzed by immunoblotting using affinity-purified antibodies raised against recombinant BIO3-BIO1 (BIO3-BIO1 Ab) or preimmune serum (for negative control). (B) Immunolocalization of BIO3-BIO1 in Arabidopsis . Soluble proteins (50 µg) from total plant extracts (T), chloroplast stroma (St), mitochondrial matrix (Ma), and cytosolic enriched fraction (Cy) were separated by SDS-PAGE and analyzed by immunoblotting as in (A) . Arrows in (A) and (B) point to the BIO3-BIO1 polypeptide band. (C) in Arabidopsis fused to the C terminus of the small subunit of ribulose-1,5-bis-phosphate carboxylase/oxygenase transit peptide (a chloroplastic marker) from Arabidopsis fused to the C terminus of the dihydropterin pyrophosphokinase-dihydropteroate synthase transit peptide (a mitochondrial marker) from pea ( Pisum sativum fused to the C terminus of full-length BIO3-BIO1 (BIO3-BIO1) were introduced into Arabidopsis ; green pseudocolor), and chlorophyll fluorescence (Chlorophyll; red pseudocolor). Bar = 10 µm.

    Journal: The Plant Cell

    Article Title: Biochemical and Structural Characterization of the Arabidopsis Bifunctional Enzyme Dethiobiotin Synthetase-Diaminopelargonic Acid Aminotransferase: Evidence for Substrate Channeling in Biotin Synthesis [C] Bifunctional Enzyme Dethiobiotin Synthetase-Diaminopelargonic Acid Aminotransferase: Evidence for Substrate Channeling in Biotin Synthesis [C] [W]

    doi: 10.1105/tpc.112.097675

    Figure Lengend Snippet: Analysis of the Expression of the Arabidopsis BIO3-BIO1 Locus at the Protein Level and Subcellular Distribution. (A) Immunological detection of BIO3-BIO1 gene products in Arabidopsis . Soluble proteins (40 µg) from aboveground organs from Arabidopsis plants (lane 1) and Arabidopsis cultured cells (lane 2) were separated by SDS-PAGE and analyzed by immunoblotting using affinity-purified antibodies raised against recombinant BIO3-BIO1 (BIO3-BIO1 Ab) or preimmune serum (for negative control). (B) Immunolocalization of BIO3-BIO1 in Arabidopsis . Soluble proteins (50 µg) from total plant extracts (T), chloroplast stroma (St), mitochondrial matrix (Ma), and cytosolic enriched fraction (Cy) were separated by SDS-PAGE and analyzed by immunoblotting as in (A) . Arrows in (A) and (B) point to the BIO3-BIO1 polypeptide band. (C) in Arabidopsis fused to the C terminus of the small subunit of ribulose-1,5-bis-phosphate carboxylase/oxygenase transit peptide (a chloroplastic marker) from Arabidopsis fused to the C terminus of the dihydropterin pyrophosphokinase-dihydropteroate synthase transit peptide (a mitochondrial marker) from pea ( Pisum sativum fused to the C terminus of full-length BIO3-BIO1 (BIO3-BIO1) were introduced into Arabidopsis ; green pseudocolor), and chlorophyll fluorescence (Chlorophyll; red pseudocolor). Bar = 10 µm.

    Article Snippet: Full-length monocistronic and bicistronic BIO3-BIO1 cDNAs and cDNA encoding mature BIO3-BIO1 protein (mBIO3-BIO1; residues 23 to 833) were obtained by PCR amplification of an RT reaction using total RNAs isolated from Arabidopsis seedlings (RNeasy plant mini kit from Qiagen).

    Techniques: Expressing, Cell Culture, SDS Page, Affinity Purification, Recombinant, Negative Control, Marker, Fluorescence

    View of the Overall Fold of the Dimer of mBIO3-BIO1 from Arabidopsis . is displayed as a yellow stick at the active sites.

    Journal: The Plant Cell

    Article Title: Biochemical and Structural Characterization of the Arabidopsis Bifunctional Enzyme Dethiobiotin Synthetase-Diaminopelargonic Acid Aminotransferase: Evidence for Substrate Channeling in Biotin Synthesis [C] Bifunctional Enzyme Dethiobiotin Synthetase-Diaminopelargonic Acid Aminotransferase: Evidence for Substrate Channeling in Biotin Synthesis [C] [W]

    doi: 10.1105/tpc.112.097675

    Figure Lengend Snippet: View of the Overall Fold of the Dimer of mBIO3-BIO1 from Arabidopsis . is displayed as a yellow stick at the active sites.

    Article Snippet: Full-length monocistronic and bicistronic BIO3-BIO1 cDNAs and cDNA encoding mature BIO3-BIO1 protein (mBIO3-BIO1; residues 23 to 833) were obtained by PCR amplification of an RT reaction using total RNAs isolated from Arabidopsis seedlings (RNeasy plant mini kit from Qiagen).

    Techniques:

    Physicochemical and Biochemical Properties of Recombinant Arabidopsis mBIO3-BIO1. (A) Documentation of mBIO3-BIO1 purification on nickel-nitrilotriacetic acid-agarose resin. Polypeptides were separated by SDS-PAGE and stained with Coomassie blue. Lane 1, soluble proteins (25 µg) from E. coli Rosetta cells producing mBIO3-BIO1; lane 2, proteins eluted from the column (10 µg); lanes M, molecular mass markers. (B) Purification and molecular mass estimation of native mBIO3-BIO1 by gel filtration. Purified protein was resolved by chromatography onto a Superdex 200 HiLoad column (2.6 × 60 cm; GE Healthcare). Eluted fractions (3 mL) were analyzed by SDS-PAGE (top panel). Standards proteins for column calibration (bottom panel) were as follows: thyroglobulin (669 kD), ferritin (443 kD), catalase (232 kD), γ-globulin (158 kD), aldolase (158 kD), ovalbumin (43 kD), and myoglobulin (17 kD). Ve, elution volume; V0, void volume. (C) Spectroscopy analysis of purified recombinant mBIO3-BIO1. Absorption spectrum was recorded at 30°C in 100 mM HEPES-KOH, pH 7.5, in the presence of 20 µM pure enzyme. (D) reaction were performed by measuring the formation of acid-stable [ 14 from acid-labile H 14 CO 3 reaction) enzyme, and reaction mixtures were incubated for 120 or 20 min, respectively. [ 14 ). Migration was compared with that of authentic [ 3 standard (Rf = 0.53). [See online article for color version of this figure.]

    Journal: The Plant Cell

    Article Title: Biochemical and Structural Characterization of the Arabidopsis Bifunctional Enzyme Dethiobiotin Synthetase-Diaminopelargonic Acid Aminotransferase: Evidence for Substrate Channeling in Biotin Synthesis [C] Bifunctional Enzyme Dethiobiotin Synthetase-Diaminopelargonic Acid Aminotransferase: Evidence for Substrate Channeling in Biotin Synthesis [C] [W]

    doi: 10.1105/tpc.112.097675

    Figure Lengend Snippet: Physicochemical and Biochemical Properties of Recombinant Arabidopsis mBIO3-BIO1. (A) Documentation of mBIO3-BIO1 purification on nickel-nitrilotriacetic acid-agarose resin. Polypeptides were separated by SDS-PAGE and stained with Coomassie blue. Lane 1, soluble proteins (25 µg) from E. coli Rosetta cells producing mBIO3-BIO1; lane 2, proteins eluted from the column (10 µg); lanes M, molecular mass markers. (B) Purification and molecular mass estimation of native mBIO3-BIO1 by gel filtration. Purified protein was resolved by chromatography onto a Superdex 200 HiLoad column (2.6 × 60 cm; GE Healthcare). Eluted fractions (3 mL) were analyzed by SDS-PAGE (top panel). Standards proteins for column calibration (bottom panel) were as follows: thyroglobulin (669 kD), ferritin (443 kD), catalase (232 kD), γ-globulin (158 kD), aldolase (158 kD), ovalbumin (43 kD), and myoglobulin (17 kD). Ve, elution volume; V0, void volume. (C) Spectroscopy analysis of purified recombinant mBIO3-BIO1. Absorption spectrum was recorded at 30°C in 100 mM HEPES-KOH, pH 7.5, in the presence of 20 µM pure enzyme. (D) reaction were performed by measuring the formation of acid-stable [ 14 from acid-labile H 14 CO 3 reaction) enzyme, and reaction mixtures were incubated for 120 or 20 min, respectively. [ 14 ). Migration was compared with that of authentic [ 3 standard (Rf = 0.53). [See online article for color version of this figure.]

    Article Snippet: Full-length monocistronic and bicistronic BIO3-BIO1 cDNAs and cDNA encoding mature BIO3-BIO1 protein (mBIO3-BIO1; residues 23 to 833) were obtained by PCR amplification of an RT reaction using total RNAs isolated from Arabidopsis seedlings (RNeasy plant mini kit from Qiagen).

    Techniques: Recombinant, Purification, SDS Page, Staining, Filtration, Chromatography, Spectroscopy, Incubation, Migration

    . (A) by two distinct enzymes encoded by bioA and bioD reactions. AdoMTOB, S -adenosyl-2-oxo-4-methylthiobutyric acid. (B) Representation of BIO3 (BioD ortholog) and BIO1 (BioA ortholog) domains of BIO3-BIO1 fusion protein and of separate BIO3 and BIO1 proteins, putatively encoded by Arabidopsis monocistronic and bicistronic BIO3-BIO1 transcripts, respectively. [See online article for color version of this figure.]

    Journal: The Plant Cell

    Article Title: Biochemical and Structural Characterization of the Arabidopsis Bifunctional Enzyme Dethiobiotin Synthetase-Diaminopelargonic Acid Aminotransferase: Evidence for Substrate Channeling in Biotin Synthesis [C] Bifunctional Enzyme Dethiobiotin Synthetase-Diaminopelargonic Acid Aminotransferase: Evidence for Substrate Channeling in Biotin Synthesis [C] [W]

    doi: 10.1105/tpc.112.097675

    Figure Lengend Snippet: . (A) by two distinct enzymes encoded by bioA and bioD reactions. AdoMTOB, S -adenosyl-2-oxo-4-methylthiobutyric acid. (B) Representation of BIO3 (BioD ortholog) and BIO1 (BioA ortholog) domains of BIO3-BIO1 fusion protein and of separate BIO3 and BIO1 proteins, putatively encoded by Arabidopsis monocistronic and bicistronic BIO3-BIO1 transcripts, respectively. [See online article for color version of this figure.]

    Article Snippet: Full-length monocistronic and bicistronic BIO3-BIO1 cDNAs and cDNA encoding mature BIO3-BIO1 protein (mBIO3-BIO1; residues 23 to 833) were obtained by PCR amplification of an RT reaction using total RNAs isolated from Arabidopsis seedlings (RNeasy plant mini kit from Qiagen).

    Techniques: