Journal: PLoS ONE
Article Title: Transforming Microbial Genotyping: A Robotic Pipeline for Genotyping Bacterial Strains
Figure Lengend Snippet: (A) Schematic layout of the equipment within the LHS1 cabinet. Positions A and B represent stack units where racks of 2D tubes and deep well plates are stored prior to operations. Position C designates the 5-position, XY-moveable stage for the 96-tip pipettor. Position D represents a rest position used for regripping of racks of 2D tubes before and after capping/decapping in position G. Positions E and F represent 2-D and 1-D bar-code readers. H is a SCARA robotic arm for moving between these positions. Further details of how LHS1 was used are presented in , / for sterile dispensing of media into 2D tubes, and / for aseptic sub-cultivation of bacteria into 2D tubes and deep well plates. (B) Improvements in pipetting parameters (right) which eliminated aerosol formation that led to cross-contamination between cultures (left).
Article Snippet: LHS1 consists of a 96 tip Apricot pipettor (Xpp-96H, FluidX, Cheshire, UK), a 96 tube capper/decapper (Xsd-96(F), FluidX), a SCARA robot arm (PAA, Farnborough, UK), a 1-D bar-code reader (MS7120, Orbit, Honeywell International, Morristown, New York, USA) and a 96 tube 2-D bar-code reader (Xtr-96 MKII, FluidX) ( ).