Journal: PLoS ONE

Article Title: Transforming Microbial Genotyping: A Robotic Pipeline for Genotyping Bacterial Strains

doi: 10.1371/journal.pone.0048022

Figure Lengend Snippet: (A) Schematic layout of the equipment within the LHS1 cabinet. Positions A and B represent stack units where racks of 2D tubes and deep well plates are stored prior to operations. Position C designates the 5-position, XY-moveable stage for the 96-tip pipettor. Position D represents a rest position used for regripping of racks of 2D tubes before and after capping/decapping in position G. Positions E and F represent 2-D and 1-D bar-code readers. H is a SCARA robotic arm for moving between these positions. Further details of how LHS1 was used are presented in , / for sterile dispensing of media into 2D tubes, and / for aseptic sub-cultivation of bacteria into 2D tubes and deep well plates. (B) Improvements in pipetting parameters (right) which eliminated aerosol formation that led to cross-contamination between cultures (left).

Article Snippet: LHS1 consists of a 96 tip Apricot pipettor (Xpp-96H, FluidX, Cheshire, UK), a 96 tube capper/decapper (Xsd-96(F), FluidX), a SCARA robot arm (PAA, Farnborough, UK), a 1-D bar-code reader (MS7120, Orbit, Honeywell International, Morristown, New York, USA) and a 96 tube 2-D bar-code reader (Xtr-96 MKII, FluidX) ( ).

Techniques:

Journal: bioRxiv

Article Title: Coupling comprehensive pesticide-wide association study to iPSC dopaminergic screening identifies and classifies Parkinson-relevant pesticides

doi: 10.1101/2022.02.06.479305

Figure Lengend Snippet: Direct treatment of mDA neurons and live imaging schematic. Upper . Assay design for custom PWAS in vitro dopaminergic neuron screen. Embryoid bodies/spheres were differentiated with a midbrain patterning protocol. Spheres containing THtdtomato+ dopamine neurons are dissociated for FACS between day 35-42 to purify THtdtomato+ cells which are then plated into multiwell assay plates using an Apricot Personal Pipettor, which was also utilized for media changes and toxicant treatment. Endogenous THtdtomato fluorescence was imaged on an IXM high content microscope fitted with a live cell chamber. Lower : timeline and sequencing of treatments, media changes, and imaging. Asterisks show imaging time points.

Article Snippet: Media wash and transition to Fluorobrite (GIBCO) live imaging media was performed the following day using an Apricot Personal Pipettor (Apricot Designs) leaving 90uL of fresh media per well.

Techniques: Imaging, In Vitro, Fluorescence, Microscopy, Sequencing