Journal: International Journal of Nanomedicine

Article Title: Nanoparticle BAF312@CaP-NP Overcomes Sphingosine-1-Phosphate Receptor-1-Mediated Chemoresistance Through Inhibiting S1PR1/P-STAT3 Axis in Ovarian Carcinoma

doi: 10.2147/IJN.S248667

Figure Lengend Snippet: BAF312 decreases S1PR1 and P-STAT3 levels and promotes apoptosis in SKOV3DR cells. Notes: ( A ) The chemical structure of BAF312. ( B ) MTT assay analysis of the viability of SKOV3DR cell lines after treatment with BAF312 for 72 h. ( C ) qPCR analysis of S1PR1 expression in SKOV3DR cells following treatment with 10 µM BAF312 for 2 days. Mean ± SEM, n = 3, ***P < 0.001. ( D ) Western blot analysis of the protein levels of S1PR1 and P-STAT3 in SKOV3DR cells following treatment with 10 µM BAF312 for 2 days. ( E ) MTT assay analysis of the viability of SKOV3DR-siRNA cells treated with BAF312 for 72 h. ( F ) MTT assay analysis of the viability of SKOV3DR cells treated with the indicated concentrations of DDP for 72 h. Mean ± SEM, n=5, ns P>0.05. ( G ) Apoptosis assay analysis of the apoptotic ratio of SKOV3DR cell lines after incubation with 10 µM BAF312, 10 µM DDP, and 5 µM BAF312 + 5 µM DDP for 2 days. ( H ) The statistical results of the apoptotic ratio for SKOV3DR cells. Mean ± SEM, n = 3, **P < 0.01, ***P < 0.001.

Article Snippet: Cells were seeded in 6-well plates at a density of 5×10 5 cells per well overnight and treated with the indicated concentrations of the indicated drugs for 48 h. Cells were collected and detected by an Annexin V-PE apoptosis detection kit (Vazyme, China).

Techniques: MTT Assay, Expressing, Western Blot, Apoptosis Assay, Incubation

Journal: International Journal of Nanomedicine

Article Title: Nanoparticle BAF312@CaP-NP Overcomes Sphingosine-1-Phosphate Receptor-1-Mediated Chemoresistance Through Inhibiting S1PR1/P-STAT3 Axis in Ovarian Carcinoma

doi: 10.2147/IJN.S248667

Figure Lengend Snippet: BAF312@CaP-NPs dramatically boost the apoptosis of SKOV3DR cells by inhibiting S1PR1 and downregulating P-STAT3. Notes: ( A ) Fluorescence microscopy analysis of the cellular uptake of tumor-targeted shell-core nanoparticles in SKOV3DR cell lines. ( B ) Apoptosis assay analysis of the apoptotic ratio of SKOV3DR cell lines after incubation with 10 µM BAF312, 10 µM BAF312@CaP-NPs and an equal amount of control CaP-NPs for 2 days. ( C ) The statistical results of the apoptotic ratio for SKOV3DR cells. Mean ± SEM, n = 3, ns P > 0.05, *P < 0.05, ***P < 0.001. ( D ) Calcium indicator and PI staining analysis of apoptosis in SKOV3DR cells following incubation with 10 µM BAF312, 10 µM BAF312@CaP-NPs and an equal amount of control CaP-NPs for 3 days. The red represents PI positivity, the green represents calcium positivity, and the yellow represents both PI and calcium positivity. ( E ) MTT assay analysis of the viability of SKOV3DR cells treated with BAF312 for 12 h and 24 h in pH 6.0 and pH 7.4 medium, respectively. ( F ) Western blot analysis of the protein levels of S1PR1, P-STAT3, and survivin in SKOV3DR cells following treatment with 10 µM BAF312, 10 µM BAF312@CaP-NPs and an equal amount of control CaP-NPs for 2 days. ( G ) Schematic diagram of BAF312@CaP-NPs killing ovarian cancer cells.

Article Snippet: Cells were seeded in 6-well plates at a density of 5×10 5 cells per well overnight and treated with the indicated concentrations of the indicated drugs for 48 h. Cells were collected and detected by an Annexin V-PE apoptosis detection kit (Vazyme, China).

Techniques: Fluorescence, Microscopy, Apoptosis Assay, Incubation, Staining, MTT Assay, Western Blot

Journal: Biomolecules

Article Title: Crinamine Induces Apoptosis and Inhibits Proliferation, Migration, and Angiogenesis in Cervical Cancer SiHa Cells

doi: 10.3390/biom9090494

Figure Lengend Snippet: Crinamine-mediated apoptosis activation in cervical cancer cells. ( a ) Annexin V and PI staining of SiHa cells treated with cisplatin, crinamine, or DMSO for 16 h. ( b ) Fluorescent intensity of Annexin V staining from ( a ); results are shown as means ± SD from triplicate measurements. ( c ) Caspase-3/7 activity of crinamine in SiHa cells non-treated or treated with the indicated concentrations of cisplatin or crinamine for 24 h. Caspase activity is normalized to the DMSO control and is reported as means ± SD from triplicate experiments. ( d ) Immunofluorescence staining of histone γ-H2AX in SiHa cells treated with the indicated concentrations of cisplatin, crinamine, or DMSO for 4 and 24 h. Panels represent phase contrast (PhL) images, DAPI nuclear staining (blue), γ-H2AX foci (red), and merged images. ( e ) Percentage of γ-H2AX foci-positive cells quantified from five fields per treatment obtained from duplicate measurements. Results are representative of data observed on two separate occasions. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: SiHa cells were seeded in 24-well plates and treated with the indicated concentration of cisplatin, crinamine, or 0.16% DMSO for 16 h. Cells were stained with Annexin V-fluorescein isothiocyanate (FITC) using the Annexin V-FITC Early Apoptosis Detection Kit (#6592; Cell Signaling Technology, Danvers, MA, USA).

Techniques: Activation Assay, Staining, Activity Assay, Immunofluorescence

Journal: Drug Delivery

Article Title: A recombinant scFv antibody-based fusion protein that targets EGFR associated with IMPDH2 downregulation and its drug conjugate show therapeutic efficacy against esophageal cancer

doi: 10.1080/10717544.2022.2063454

Figure Lengend Snippet: DNA damage, cell cycle arrest analysis, and apoptosis of EC cells. (A,B) Flow cytometry analyses of apoptosis in KYSE150 and Eca109 cells treated with Fv-LDP-D3 and Fv-LDP-D3-AE, respectively. (C) Western blot analysis of apoptosis-related proteins in KYSE150 cells treated with Fv-LDP-D3. (D) Western blot analysis of apoptosis- and DNA damage-related protein expression in KYSE150 and Eca109 cells treated with Fv-LDP-D3-AE. (E) Cell cycle arrest in KYSE150 and Eca109 cells treated with Fv-LDP-D3-AE was determined by flow cytometry. Results are reported as means ± standard deviation (SD) ( n = 3). ** p < 0.01, *** p < 0.001 versus control group.

Article Snippet: Cells were collected according to the instructions of the Annexin V FITC Apoptosis Detection Kit (Dojindo).

Techniques: Flow Cytometry, Western Blot, Expressing, Standard Deviation