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  • 97
    Vazyme Biotech Co annexin v
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    TaKaRa dutp biotin nick end labeling tunel
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    Cell Signaling Technology Inc annexin v fitc early apoptosis detection kit
    Crinamine-mediated <t>apoptosis</t> activation in cervical cancer cells. ( a ) <t>Annexin</t> <t>V</t> and PI staining of SiHa cells treated with cisplatin, crinamine, or DMSO for 16 h. ( b ) Fluorescent intensity of Annexin V staining from ( a ); results are shown as means ± SD from triplicate measurements. ( c ) Caspase-3/7 activity of crinamine in SiHa cells non-treated or treated with the indicated concentrations of cisplatin or crinamine for 24 h. Caspase activity is normalized to the DMSO control and is reported as means ± SD from triplicate experiments. ( d ) Immunofluorescence staining of histone γ-H2AX in SiHa cells treated with the indicated concentrations of cisplatin, crinamine, or DMSO for 4 and 24 h. Panels represent phase contrast (PhL) images, DAPI nuclear staining (blue), γ-H2AX foci (red), and merged images. ( e ) Percentage of γ-H2AX foci-positive cells quantified from five fields per treatment obtained from duplicate measurements. Results are representative of data observed on two separate occasions. * p < 0.05; ** p < 0.01; *** p < 0.001.
    Annexin V Fitc Early Apoptosis Detection Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/annexin v fitc early apoptosis detection kit/product/Cell Signaling Technology Inc
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    Dojindo Labs annexin v fitc apoptosis detection kit
    Crinamine-mediated <t>apoptosis</t> activation in cervical cancer cells. ( a ) <t>Annexin</t> <t>V</t> and PI staining of SiHa cells treated with cisplatin, crinamine, or DMSO for 16 h. ( b ) Fluorescent intensity of Annexin V staining from ( a ); results are shown as means ± SD from triplicate measurements. ( c ) Caspase-3/7 activity of crinamine in SiHa cells non-treated or treated with the indicated concentrations of cisplatin or crinamine for 24 h. Caspase activity is normalized to the DMSO control and is reported as means ± SD from triplicate experiments. ( d ) Immunofluorescence staining of histone γ-H2AX in SiHa cells treated with the indicated concentrations of cisplatin, crinamine, or DMSO for 4 and 24 h. Panels represent phase contrast (PhL) images, DAPI nuclear staining (blue), γ-H2AX foci (red), and merged images. ( e ) Percentage of γ-H2AX foci-positive cells quantified from five fields per treatment obtained from duplicate measurements. Results are representative of data observed on two separate occasions. * p < 0.05; ** p < 0.01; *** p < 0.001.
    Annexin V Fitc Apoptosis Detection Kit, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Crinamine-mediated apoptosis activation in cervical cancer cells. ( a ) Annexin V and PI staining of SiHa cells treated with cisplatin, crinamine, or DMSO for 16 h. ( b ) Fluorescent intensity of Annexin V staining from ( a ); results are shown as means ± SD from triplicate measurements. ( c ) Caspase-3/7 activity of crinamine in SiHa cells non-treated or treated with the indicated concentrations of cisplatin or crinamine for 24 h. Caspase activity is normalized to the DMSO control and is reported as means ± SD from triplicate experiments. ( d ) Immunofluorescence staining of histone γ-H2AX in SiHa cells treated with the indicated concentrations of cisplatin, crinamine, or DMSO for 4 and 24 h. Panels represent phase contrast (PhL) images, DAPI nuclear staining (blue), γ-H2AX foci (red), and merged images. ( e ) Percentage of γ-H2AX foci-positive cells quantified from five fields per treatment obtained from duplicate measurements. Results are representative of data observed on two separate occasions. * p < 0.05; ** p < 0.01; *** p < 0.001.

    Journal: Biomolecules

    Article Title: Crinamine Induces Apoptosis and Inhibits Proliferation, Migration, and Angiogenesis in Cervical Cancer SiHa Cells

    doi: 10.3390/biom9090494

    Figure Lengend Snippet: Crinamine-mediated apoptosis activation in cervical cancer cells. ( a ) Annexin V and PI staining of SiHa cells treated with cisplatin, crinamine, or DMSO for 16 h. ( b ) Fluorescent intensity of Annexin V staining from ( a ); results are shown as means ± SD from triplicate measurements. ( c ) Caspase-3/7 activity of crinamine in SiHa cells non-treated or treated with the indicated concentrations of cisplatin or crinamine for 24 h. Caspase activity is normalized to the DMSO control and is reported as means ± SD from triplicate experiments. ( d ) Immunofluorescence staining of histone γ-H2AX in SiHa cells treated with the indicated concentrations of cisplatin, crinamine, or DMSO for 4 and 24 h. Panels represent phase contrast (PhL) images, DAPI nuclear staining (blue), γ-H2AX foci (red), and merged images. ( e ) Percentage of γ-H2AX foci-positive cells quantified from five fields per treatment obtained from duplicate measurements. Results are representative of data observed on two separate occasions. * p < 0.05; ** p < 0.01; *** p < 0.001.

    Article Snippet: SiHa cells were seeded in 24-well plates and treated with the indicated concentration of cisplatin, crinamine, or 0.16% DMSO for 16 h. Cells were stained with Annexin V-fluorescein isothiocyanate (FITC) using the Annexin V-FITC Early Apoptosis Detection Kit (#6592; Cell Signaling Technology, Danvers, MA, USA).

    Techniques: Activation Assay, Staining, Activity Assay, Immunofluorescence