Journal: Frontiers in Oncology

Article Title: CD47 Enhances Cell Viability and Migration Ability but Inhibits Apoptosis in Endometrial Carcinoma Cells via the PI3K/Akt/mTOR Signaling Pathway

doi: 10.3389/fonc.2020.01525

Figure Lengend Snippet: CD47 up-regulation inhibited apoptosis in HEC-1A and Ishikawa cells. (A,B) Up-regulation of CD47 inhibited cell apoptosis in Ishikawa cell line. (C,D) CD47 overexpression inhibited cell apoptosis in HEC-1A cell line. * p < 0.05.

Article Snippet: The cell cycle of transfected HEC-1A and Ishikawa cells were analyzed using the Cell Cycle and Apoptosis Analysis Kit (Beyotime Biotechnology, Shanghai, China), according to the manufacturer's protocol.

Techniques: Over Expression

Journal: Journal of Nanobiotechnology

Article Title: Targeting delivery of miR-146a via IMTP modified milk exosomes exerted cardioprotective effects by inhibiting NF-κB signaling pathway after myocardial ischemia-reperfusion injury

doi: 10.1186/s12951-024-02631-0

Figure Lengend Snippet: MEs-miR-146a protects H9c2 cells from OGD/R induced damage. ( A ) After 24 h of incubation, PKH26 labeled exosomes could be uptaken up by H9c2. Scale bar = 20 μm. ( B ) MEs-miR-146a decreased OGD/R induced cell apoptosis of H9c2 according to TUNEL assay. Scale bar = 50 μm. * P < 0.05, ** P < 0.01 versus the control group; & P < 0.05, && P < 0.01 versus the OGD/R group; ▲ P < 0.05 versus the OGD/R-MEs group. n = 6

Article Snippet: The one-step TUNEL Apoptosis Detection Kit (C1088, Green Fluorescence, Beyotime) was used to incubate for 60 min at RT, following by nuclear labeling with DAPI (H-1200-10, Vector Laboratories, Burlingame, CA, USA), both in the dark.

Techniques: Incubation, Labeling, TUNEL Assay, Control

Journal: Journal of Nanobiotechnology

Article Title: Targeting delivery of miR-146a via IMTP modified milk exosomes exerted cardioprotective effects by inhibiting NF-κB signaling pathway after myocardial ischemia-reperfusion injury

doi: 10.1186/s12951-024-02631-0

Figure Lengend Snippet: MEs-miR-146a protects NRCM cells from OGD/R induced damage. ( A ) Representative fluorescence images indicated that PKH26 labeled exosomes were taken up by NRCM cells after 24 h of incubation. Scale bar = 20 μm. ( B ) MEs-miR-146a decreased OGD/R induced cell apoptosis of NRCM according to TUNEL assay. Scale bar = 50 μm. ** P < 0.01 versus the control group; & P < 0.05, && P < 0.01 versus the OGD/R group; ▲ P < 0.05 versus the OGD/R-MEs group. n = 6

Article Snippet: The one-step TUNEL Apoptosis Detection Kit (C1088, Green Fluorescence, Beyotime) was used to incubate for 60 min at RT, following by nuclear labeling with DAPI (H-1200-10, Vector Laboratories, Burlingame, CA, USA), both in the dark.

Techniques: Fluorescence, Labeling, Incubation, TUNEL Assay, Control

Journal: Journal of Nanobiotechnology

Article Title: Targeting delivery of miR-146a via IMTP modified milk exosomes exerted cardioprotective effects by inhibiting NF-κB signaling pathway after myocardial ischemia-reperfusion injury

doi: 10.1186/s12951-024-02631-0

Figure Lengend Snippet: MEs-miR-146a exhibited better therapeutic efficacy in decreasing apoptotic cells and limiting inflammation than MEs. ( A - B ) Representative TUNEL staining images and quantification of apoptotic radio. ** P < 0.01 versus the Sham group; & P < 0.05 and && P < 0.01 versus the MIRI group. ( C - F ) The expression of inflammatory factors of rat serum were detected by ELISA. * P < 0.05, ** P < 0.01 versus the Sham group; & P < 0.05, && P < 0.01 versus the MIRI group; ▲ P < 0.05, ▲▲ P < 0.01 versus the MIRI-MEs-miR-146a group. n = 5

Article Snippet: The one-step TUNEL Apoptosis Detection Kit (C1088, Green Fluorescence, Beyotime) was used to incubate for 60 min at RT, following by nuclear labeling with DAPI (H-1200-10, Vector Laboratories, Burlingame, CA, USA), both in the dark.

Techniques: Drug discovery, TUNEL Assay, Staining, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Journal of Nanobiotechnology

Article Title: Targeting delivery of miR-146a via IMTP modified milk exosomes exerted cardioprotective effects by inhibiting NF-κB signaling pathway after myocardial ischemia-reperfusion injury

doi: 10.1186/s12951-024-02631-0

Figure Lengend Snippet: Tail vein injection of IMTP-MEs-miR-146a significantly ameliorated myocardium apoptosis and attenuated inflammation at the early stage of MIRI. ( A ) Representative H&E images of heart tissue from each group. Scale bar = 50 μm. ( B - C ) Representative images of TUNEL staining 24 h after MIRI and quantitative analysis of apoptotic radio. * P < 0.05 and ** P < 0.01 versus the MIRI group; & P < 0.05 versus the MIRI-MEs-miR-146a group. n = 8. ( D - G ) The expression of inflammatory factors of rat serum were detected by ELISA. ∆∆ P < 0.01 versus the MIRI group; ▲▲ P < 0.01 versus the MIRI-MEs-miR-146a group. n = 8

Article Snippet: The one-step TUNEL Apoptosis Detection Kit (C1088, Green Fluorescence, Beyotime) was used to incubate for 60 min at RT, following by nuclear labeling with DAPI (H-1200-10, Vector Laboratories, Burlingame, CA, USA), both in the dark.

Techniques: Injection, TUNEL Assay, Staining, Expressing, Enzyme-linked Immunosorbent Assay

Journal: The Kaohsiung Journal of Medical Sciences

Article Title: lncRNA NEAT1 promotes hypoxia‐induced inflammation and fibrosis of alveolar epithelial cells via targeting miR ‐29a/ NFATc3 axis

doi: 10.1002/kjm2.12535

Figure Lengend Snippet: Knockdown of NEAT1 alleviated hypoxia‐stimulated inflammatory responses, apoptosis and fibrosis in pulmonary alveolar epithelial cells. HPAEpic and A549 cells were transfected with NEAT1 shRNA (sh‐NEAT1) and negative control shRNA (sh‐NC) and then exposed to hypoxic stimulation. (A) qRT–PCR analysis of NEAT1 levels. (B) Cell apoptosis was analyzed by a TUNEL assay. (C) The levels of inflammatory factors, including TNF‐α, IL‐1β, and IL‐6, in HPAEpic and A549 cells were analyzed by ELISAs. (D) Western blot analysis of the expression of the fibrosis‐associated proteins, including α‐SMA, collagen I and collagen III. Data represent the mean ± SD ( n = 3 independent experiments). * p < 0.05; ** p < 0.01; *** p < 0.001. Hx, Hypoxia; Nx, Normoxia

Article Snippet: Cell apoptosis was monitored by TUNEL Apoptosis Assay Kits (Beyotime Biotech).

Techniques: Knockdown, Transfection, shRNA, Negative Control, Quantitative RT-PCR, TUNEL Assay, Western Blot, Expressing

Journal: The Kaohsiung Journal of Medical Sciences

Article Title: lncRNA NEAT1 promotes hypoxia‐induced inflammation and fibrosis of alveolar epithelial cells via targeting miR ‐29a/ NFATc3 axis

doi: 10.1002/kjm2.12535

Figure Lengend Snippet: miR‐29a/NFATc3 axis was participated in the regulation of NEAT1 in hypoxia‐induced inflammation and fibrosis. HPAEpic and A549 cells were transfected with sh‐NEAT1 + miR‐29a inhibitor or sh‐NEAT1 + pcDNA‐NFATc3 and further exposed to hypoxic stimulation. (A,B) qRT–PCR analyzed the expression of miR‐29a and NFATc3 in HPAEpic and A549 cells. (C) TUNEL assays detected the cell apoptosis. (D) The levels of inflammatory factors, including TNF‐α, IL‐1β, and IL‐6, in HPAEpic and A549 cells were detected by ELISAs. (E) Western blot analysis of the expression of the fibrosis‐associated proteins, including α‐SMA, collagen I and collagen III. Data represent the mean ± SD ( n = 3 independent experiments). * p < 0.05; ** p < 0.01; *** p < 0.001. Hx, Hypoxia; Nx, Normoxia

Article Snippet: Cell apoptosis was monitored by TUNEL Apoptosis Assay Kits (Beyotime Biotech).

Techniques: Transfection, Quantitative RT-PCR, Expressing, TUNEL Assay, Western Blot