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  • 95
    New England Biolabs apo i
    c idA _IV gene polymorphic regions and <t>PCR-RFLP</t> tests for specific variants. a Schematic representation of the architecture of cidA _IV polymorphism. The black line represents the cidA_IV sequence with a focus on the two polymorphic regions. Non-polymorphic regions were shortened and represented as a dashed line. Numbers in red under the line represent nucleotide positions, and the numbers in blue indicate amino-acid positions. The overlapping oligonucleotides used for PCR amplification are represented by arrows numbered as in Supplementary Table 2 . Both polymorphic regions were studied, but only the upstream matched the compatibility profile; its four different sequences (α, β γ, and δ) were followed by one of the two possible sequences (1 or 2) in the downstream polymorphic region. A different color code was used for α (purple), β (light blue), γ (light green), and δ (yellow) sequences in the upstream part of the cidA gene. b The repertoire of cidA _IV variants is different in “compatible” and “incompatible” lines. cidA _IV(δ) is present only in lines with “incompatible” crossing type. The names of the C . pipiens lines used to set-up the PCR-RFLP ( c ) test are highlighted in red. c PCR-RFLP tests for distinguishing between cidA _IV variants on the basis of the upstream polymorphic region. A 778 bp fragment was amplified with primers 1/13. Double digestion with <t>Apo</t> I and Hpy 188I distinguished between cidA _IV(α) (six fragments: 471; 122; 57; 53; 51; and 24 bp), cidA _IV(β) and cidA _IV(γ) (six fragments: 441; 122; 83; 57; 51; and 24 bp), and cidA _IV(δ) (five fragments: 524; 122; 57; 51; and 24 bp). Panel to the left of the electrophoresis gel: PCR-RFLP on DNA from clones; right panel: PCR-RFLP on DNA from the Istanbul, Harash, Ichkeul 13, and Ichkeul 09 lines. On the right of the gel, a schematic representation of the PCR-RFLP profiles of these lines. Digestion bands that are specific of the variants are represented with the color code established in a . Bands that are not used to discriminate between the variants are represented in black on the bottom of the schematic gel
    Apo I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 130 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apo i/product/New England Biolabs
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    91
    Thermo Fisher xapi apoi
    c idA _IV gene polymorphic regions and <t>PCR-RFLP</t> tests for specific variants. a Schematic representation of the architecture of cidA _IV polymorphism. The black line represents the cidA_IV sequence with a focus on the two polymorphic regions. Non-polymorphic regions were shortened and represented as a dashed line. Numbers in red under the line represent nucleotide positions, and the numbers in blue indicate amino-acid positions. The overlapping oligonucleotides used for PCR amplification are represented by arrows numbered as in Supplementary Table 2 . Both polymorphic regions were studied, but only the upstream matched the compatibility profile; its four different sequences (α, β γ, and δ) were followed by one of the two possible sequences (1 or 2) in the downstream polymorphic region. A different color code was used for α (purple), β (light blue), γ (light green), and δ (yellow) sequences in the upstream part of the cidA gene. b The repertoire of cidA _IV variants is different in “compatible” and “incompatible” lines. cidA _IV(δ) is present only in lines with “incompatible” crossing type. The names of the C . pipiens lines used to set-up the PCR-RFLP ( c ) test are highlighted in red. c PCR-RFLP tests for distinguishing between cidA _IV variants on the basis of the upstream polymorphic region. A 778 bp fragment was amplified with primers 1/13. Double digestion with <t>Apo</t> I and Hpy 188I distinguished between cidA _IV(α) (six fragments: 471; 122; 57; 53; 51; and 24 bp), cidA _IV(β) and cidA _IV(γ) (six fragments: 441; 122; 83; 57; 51; and 24 bp), and cidA _IV(δ) (five fragments: 524; 122; 57; 51; and 24 bp). Panel to the left of the electrophoresis gel: PCR-RFLP on DNA from clones; right panel: PCR-RFLP on DNA from the Istanbul, Harash, Ichkeul 13, and Ichkeul 09 lines. On the right of the gel, a schematic representation of the PCR-RFLP profiles of these lines. Digestion bands that are specific of the variants are represented with the color code established in a . Bands that are not used to discriminate between the variants are represented in black on the bottom of the schematic gel
    Xapi Apoi, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Thermo Fisher apo i
    c idA _IV gene polymorphic regions and <t>PCR-RFLP</t> tests for specific variants. a Schematic representation of the architecture of cidA _IV polymorphism. The black line represents the cidA_IV sequence with a focus on the two polymorphic regions. Non-polymorphic regions were shortened and represented as a dashed line. Numbers in red under the line represent nucleotide positions, and the numbers in blue indicate amino-acid positions. The overlapping oligonucleotides used for PCR amplification are represented by arrows numbered as in Supplementary Table 2 . Both polymorphic regions were studied, but only the upstream matched the compatibility profile; its four different sequences (α, β γ, and δ) were followed by one of the two possible sequences (1 or 2) in the downstream polymorphic region. A different color code was used for α (purple), β (light blue), γ (light green), and δ (yellow) sequences in the upstream part of the cidA gene. b The repertoire of cidA _IV variants is different in “compatible” and “incompatible” lines. cidA _IV(δ) is present only in lines with “incompatible” crossing type. The names of the C . pipiens lines used to set-up the PCR-RFLP ( c ) test are highlighted in red. c PCR-RFLP tests for distinguishing between cidA _IV variants on the basis of the upstream polymorphic region. A 778 bp fragment was amplified with primers 1/13. Double digestion with <t>Apo</t> I and Hpy 188I distinguished between cidA _IV(α) (six fragments: 471; 122; 57; 53; 51; and 24 bp), cidA _IV(β) and cidA _IV(γ) (six fragments: 441; 122; 83; 57; 51; and 24 bp), and cidA _IV(δ) (five fragments: 524; 122; 57; 51; and 24 bp). Panel to the left of the electrophoresis gel: PCR-RFLP on DNA from clones; right panel: PCR-RFLP on DNA from the Istanbul, Harash, Ichkeul 13, and Ichkeul 09 lines. On the right of the gel, a schematic representation of the PCR-RFLP profiles of these lines. Digestion bands that are specific of the variants are represented with the color code established in a . Bands that are not used to discriminate between the variants are represented in black on the bottom of the schematic gel
    Apo I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apo i/product/Thermo Fisher
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    85
    Thermo Fisher apo i afl iii digested pzero 2
    c idA _IV gene polymorphic regions and <t>PCR-RFLP</t> tests for specific variants. a Schematic representation of the architecture of cidA _IV polymorphism. The black line represents the cidA_IV sequence with a focus on the two polymorphic regions. Non-polymorphic regions were shortened and represented as a dashed line. Numbers in red under the line represent nucleotide positions, and the numbers in blue indicate amino-acid positions. The overlapping oligonucleotides used for PCR amplification are represented by arrows numbered as in Supplementary Table 2 . Both polymorphic regions were studied, but only the upstream matched the compatibility profile; its four different sequences (α, β γ, and δ) were followed by one of the two possible sequences (1 or 2) in the downstream polymorphic region. A different color code was used for α (purple), β (light blue), γ (light green), and δ (yellow) sequences in the upstream part of the cidA gene. b The repertoire of cidA _IV variants is different in “compatible” and “incompatible” lines. cidA _IV(δ) is present only in lines with “incompatible” crossing type. The names of the C . pipiens lines used to set-up the PCR-RFLP ( c ) test are highlighted in red. c PCR-RFLP tests for distinguishing between cidA _IV variants on the basis of the upstream polymorphic region. A 778 bp fragment was amplified with primers 1/13. Double digestion with <t>Apo</t> I and Hpy 188I distinguished between cidA _IV(α) (six fragments: 471; 122; 57; 53; 51; and 24 bp), cidA _IV(β) and cidA _IV(γ) (six fragments: 441; 122; 83; 57; 51; and 24 bp), and cidA _IV(δ) (five fragments: 524; 122; 57; 51; and 24 bp). Panel to the left of the electrophoresis gel: PCR-RFLP on DNA from clones; right panel: PCR-RFLP on DNA from the Istanbul, Harash, Ichkeul 13, and Ichkeul 09 lines. On the right of the gel, a schematic representation of the PCR-RFLP profiles of these lines. Digestion bands that are specific of the variants are represented with the color code established in a . Bands that are not used to discriminate between the variants are represented in black on the bottom of the schematic gel
    Apo I Afl Iii Digested Pzero 2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Thermo Fisher apo i restriction enzyme
    Identification of the four haplotypes by enzyme digestion. Lanes 1 , 3 , 5 and 7 were the nested PCR product of the four haplotypes. Lanes 2 , 4 , 6 and 8 were digested by <t>Apo</t> I. M, molecular marker; lanes 1 and 2 , CVMNK; lanes 3 and 4 , CVM/I N/E/D/K T/K; lanes 5 and 6 , CVIET; lanes 7 and 8 , CVIEK
    Apo I Restriction Enzyme, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore ns3694 apo i
    Identification of the four haplotypes by enzyme digestion. Lanes 1 , 3 , 5 and 7 were the nested PCR product of the four haplotypes. Lanes 2 , 4 , 6 and 8 were digested by <t>Apo</t> I. M, molecular marker; lanes 1 and 2 , CVMNK; lanes 3 and 4 , CVM/I N/E/D/K T/K; lanes 5 and 6 , CVIET; lanes 7 and 8 , CVIEK
    Ns3694 Apo I, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    c idA _IV gene polymorphic regions and PCR-RFLP tests for specific variants. a Schematic representation of the architecture of cidA _IV polymorphism. The black line represents the cidA_IV sequence with a focus on the two polymorphic regions. Non-polymorphic regions were shortened and represented as a dashed line. Numbers in red under the line represent nucleotide positions, and the numbers in blue indicate amino-acid positions. The overlapping oligonucleotides used for PCR amplification are represented by arrows numbered as in Supplementary Table 2 . Both polymorphic regions were studied, but only the upstream matched the compatibility profile; its four different sequences (α, β γ, and δ) were followed by one of the two possible sequences (1 or 2) in the downstream polymorphic region. A different color code was used for α (purple), β (light blue), γ (light green), and δ (yellow) sequences in the upstream part of the cidA gene. b The repertoire of cidA _IV variants is different in “compatible” and “incompatible” lines. cidA _IV(δ) is present only in lines with “incompatible” crossing type. The names of the C . pipiens lines used to set-up the PCR-RFLP ( c ) test are highlighted in red. c PCR-RFLP tests for distinguishing between cidA _IV variants on the basis of the upstream polymorphic region. A 778 bp fragment was amplified with primers 1/13. Double digestion with Apo I and Hpy 188I distinguished between cidA _IV(α) (six fragments: 471; 122; 57; 53; 51; and 24 bp), cidA _IV(β) and cidA _IV(γ) (six fragments: 441; 122; 83; 57; 51; and 24 bp), and cidA _IV(δ) (five fragments: 524; 122; 57; 51; and 24 bp). Panel to the left of the electrophoresis gel: PCR-RFLP on DNA from clones; right panel: PCR-RFLP on DNA from the Istanbul, Harash, Ichkeul 13, and Ichkeul 09 lines. On the right of the gel, a schematic representation of the PCR-RFLP profiles of these lines. Digestion bands that are specific of the variants are represented with the color code established in a . Bands that are not used to discriminate between the variants are represented in black on the bottom of the schematic gel

    Journal: Nature Communications

    Article Title: Culex pipiens crossing type diversity is governed by an amplified and polymorphic operon of Wolbachia

    doi: 10.1038/s41467-017-02749-w

    Figure Lengend Snippet: c idA _IV gene polymorphic regions and PCR-RFLP tests for specific variants. a Schematic representation of the architecture of cidA _IV polymorphism. The black line represents the cidA_IV sequence with a focus on the two polymorphic regions. Non-polymorphic regions were shortened and represented as a dashed line. Numbers in red under the line represent nucleotide positions, and the numbers in blue indicate amino-acid positions. The overlapping oligonucleotides used for PCR amplification are represented by arrows numbered as in Supplementary Table 2 . Both polymorphic regions were studied, but only the upstream matched the compatibility profile; its four different sequences (α, β γ, and δ) were followed by one of the two possible sequences (1 or 2) in the downstream polymorphic region. A different color code was used for α (purple), β (light blue), γ (light green), and δ (yellow) sequences in the upstream part of the cidA gene. b The repertoire of cidA _IV variants is different in “compatible” and “incompatible” lines. cidA _IV(δ) is present only in lines with “incompatible” crossing type. The names of the C . pipiens lines used to set-up the PCR-RFLP ( c ) test are highlighted in red. c PCR-RFLP tests for distinguishing between cidA _IV variants on the basis of the upstream polymorphic region. A 778 bp fragment was amplified with primers 1/13. Double digestion with Apo I and Hpy 188I distinguished between cidA _IV(α) (six fragments: 471; 122; 57; 53; 51; and 24 bp), cidA _IV(β) and cidA _IV(γ) (six fragments: 441; 122; 83; 57; 51; and 24 bp), and cidA _IV(δ) (five fragments: 524; 122; 57; 51; and 24 bp). Panel to the left of the electrophoresis gel: PCR-RFLP on DNA from clones; right panel: PCR-RFLP on DNA from the Istanbul, Harash, Ichkeul 13, and Ichkeul 09 lines. On the right of the gel, a schematic representation of the PCR-RFLP profiles of these lines. Digestion bands that are specific of the variants are represented with the color code established in a . Bands that are not used to discriminate between the variants are represented in black on the bottom of the schematic gel

    Article Snippet: Double digestion of the PCR products with Apo I and Hpy 188I (New England Biolabs) identified three groups of sequencing: cid A_IV(α) (six fragments: 471; 122; 57; 53; 51; and 24 bp); cidA _IV(β) and cidA _IV(γ) (six fragments: 441; 123; 83; 57; 51; and 24 bp); and cidA _IV(δ) (five fragments: 524; 122; 57; 51; and 24 bp).

    Techniques: Polymerase Chain Reaction, Sequencing, Amplification, Electrophoresis, Clone Assay

    Identification of the four haplotypes by enzyme digestion. Lanes 1 , 3 , 5 and 7 were the nested PCR product of the four haplotypes. Lanes 2 , 4 , 6 and 8 were digested by Apo I. M, molecular marker; lanes 1 and 2 , CVMNK; lanes 3 and 4 , CVM/I N/E/D/K T/K; lanes 5 and 6 , CVIET; lanes 7 and 8 , CVIEK

    Journal: Malaria Journal

    Article Title: Molecular mutation profile of pfcrt in Plasmodium falciparum isolates imported from Africa in Henan province

    doi: 10.1186/s12936-016-1306-6

    Figure Lengend Snippet: Identification of the four haplotypes by enzyme digestion. Lanes 1 , 3 , 5 and 7 were the nested PCR product of the four haplotypes. Lanes 2 , 4 , 6 and 8 were digested by Apo I. M, molecular marker; lanes 1 and 2 , CVMNK; lanes 3 and 4 , CVM/I N/E/D/K T/K; lanes 5 and 6 , CVIET; lanes 7 and 8 , CVIEK

    Article Snippet: Restriction digest of pfcrt gene amplicons Enzymatic digestion of the resulting 145 base pair fragment of the pfcrt amplicons was done using Apo I restriction enzyme (Fermentas Life Sciences) according to the manufacturer’s instructions.

    Techniques: Nested PCR, Marker