Journal: The American Journal of Pathology
Article Title: Genetic Susceptibility to Experimental Autoimmune Glomerulonephritis in the Wistar Kyoto Rat
Figure Lengend Snippet: Analysis of Fc receptor–mediated macrophage activation and function in WKY, LEW, and WKY. LCrgn1 congenic rats, showing ( A ) Fc oxyburst assay, ( B ) bead phagocytosis assay, ( C ) MCP-1 production after stimulation by LPS, and ( D ) iNOS mRNA expression after stimulation by LPS Results shown represent the mean ± SD for each group. (* P
Article Snippet: Briefly, cells (1 × 106 macrophages) were harvested and resuspended in Krebs-Ringer PBS (KRP buffer) with 1.0 mmol/L Ca2+ , 1.5 mmol/L Mg2+ , and 5.5 mmol/L glucose, warmed to 37°C for 10 minutes, then stimulated with the Fc OxyBURST immune complex (120 μg/mL; Invitrogen).
Techniques: Activation Assay, Phagocytosis Assay, Expressing