antigen-antibody complexes Search Results


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  • 91
    Millipore α ha immune complexes
    Validation of subcellular localization predictions using <t>α-HA</t> immunofluorescence The indicated bait proteins fused at their C-terminus with an HA tag were expressed after transient infection of lentiviruses at low multiplicity of infection and after 2 days, cells were fixed and subjected to α-HA-based immunofluorescence (red). Nuclei were stained with Hoechst. For baits with predicted mitochondrial localization, cells were co-stained with α-TOMM20 antibodies (green). Z-series optical sections were acquired via spinning disk confocal microscopy; maximum intensity projections are shown. Scale bar=20 μm.
    α Ha Immune Complexes, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Alpha Diagnostics immune complexes cic
    Validation of subcellular localization predictions using <t>α-HA</t> immunofluorescence The indicated bait proteins fused at their C-terminus with an HA tag were expressed after transient infection of lentiviruses at low multiplicity of infection and after 2 days, cells were fixed and subjected to α-HA-based immunofluorescence (red). Nuclei were stained with Hoechst. For baits with predicted mitochondrial localization, cells were co-stained with α-TOMM20 antibodies (green). Z-series optical sections were acquired via spinning disk confocal microscopy; maximum intensity projections are shown. Scale bar=20 μm.
    Immune Complexes Cic, supplied by Alpha Diagnostics, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    IGAN Bio mesangial immune complexes
    Validation of subcellular localization predictions using <t>α-HA</t> immunofluorescence The indicated bait proteins fused at their C-terminus with an HA tag were expressed after transient infection of lentiviruses at low multiplicity of infection and after 2 days, cells were fixed and subjected to α-HA-based immunofluorescence (red). Nuclei were stained with Hoechst. For baits with predicted mitochondrial localization, cells were co-stained with α-TOMM20 antibodies (green). Z-series optical sections were acquired via spinning disk confocal microscopy; maximum intensity projections are shown. Scale bar=20 μm.
    Mesangial Immune Complexes, supplied by IGAN Bio, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher fc oxyburst immune complex
    Analysis of Fc receptor–mediated macrophage activation and function in WKY, LEW, and WKY. LCrgn1 congenic rats, showing ( A ) Fc <t>oxyburst</t> assay, ( B ) bead phagocytosis assay, ( C ) MCP-1 production after stimulation by LPS, and ( D ) iNOS mRNA expression after stimulation by LPS Results shown represent the mean ± SD for each group. (* P
    Fc Oxyburst Immune Complex, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Millipore preformed pap immune complexes
    Analysis of Fc receptor–mediated macrophage activation and function in WKY, LEW, and WKY. LCrgn1 congenic rats, showing ( A ) Fc <t>oxyburst</t> assay, ( B ) bead phagocytosis assay, ( C ) MCP-1 production after stimulation by LPS, and ( D ) iNOS mRNA expression after stimulation by LPS Results shown represent the mean ± SD for each group. (* P
    Preformed Pap Immune Complexes, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Leica Biosystems antigen antibody universal immuno peroxidase polymer complex
    Analysis of Fc receptor–mediated macrophage activation and function in WKY, LEW, and WKY. LCrgn1 congenic rats, showing ( A ) Fc <t>oxyburst</t> assay, ( B ) bead phagocytosis assay, ( C ) MCP-1 production after stimulation by LPS, and ( D ) iNOS mRNA expression after stimulation by LPS Results shown represent the mean ± SD for each group. (* P
    Antigen Antibody Universal Immuno Peroxidase Polymer Complex, supplied by Leica Biosystems, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    GE Healthcare antigen antibody complexes
    Analysis of Fc receptor–mediated macrophage activation and function in WKY, LEW, and WKY. LCrgn1 congenic rats, showing ( A ) Fc <t>oxyburst</t> assay, ( B ) bead phagocytosis assay, ( C ) MCP-1 production after stimulation by LPS, and ( D ) iNOS mRNA expression after stimulation by LPS Results shown represent the mean ± SD for each group. (* P
    Antigen Antibody Complexes, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 2946 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Upstate Biotechnology Inc licl immune complex wash buffer
    Analysis of Fc receptor–mediated macrophage activation and function in WKY, LEW, and WKY. LCrgn1 congenic rats, showing ( A ) Fc <t>oxyburst</t> assay, ( B ) bead phagocytosis assay, ( C ) MCP-1 production after stimulation by LPS, and ( D ) iNOS mRNA expression after stimulation by LPS Results shown represent the mean ± SD for each group. (* P
    Licl Immune Complex Wash Buffer, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 88/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Millipore salt immune complex wash buffer
    Analysis of Fc receptor–mediated macrophage activation and function in WKY, LEW, and WKY. LCrgn1 congenic rats, showing ( A ) Fc <t>oxyburst</t> assay, ( B ) bead phagocytosis assay, ( C ) MCP-1 production after stimulation by LPS, and ( D ) iNOS mRNA expression after stimulation by LPS Results shown represent the mean ± SD for each group. (* P
    Salt Immune Complex Wash Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Trinity Biotech mab ift immune complexes
    Administration of <t>mAb-</t> <t>iFt</t> immune complexes reverses the anti-inflammatory properties of LVS in mouse PECs ex vivo . C57BL/6 mice were immunized i.n. with PBS, i Ft (2x10 7 CFUs), or mAb- iFt , boosted on day 21 and challenged on day 35 with 10,000 CFUs of Ft LVS. After 48 hours post—LVS challenge, the PECs of immunized mice were harvested and cultured in the presence or absence of Ft . LVS at 1:10 and 1:100 MOI for 24 hrs. The cytokine production was measured as previously described. Results are representative of three independent experiments. (*) P-value
    Mab Ift Immune Complexes, supplied by Trinity Biotech, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    ALPCO immune complexes eia kits
    Administration of <t>mAb-</t> <t>iFt</t> immune complexes reverses the anti-inflammatory properties of LVS in mouse PECs ex vivo . C57BL/6 mice were immunized i.n. with PBS, i Ft (2x10 7 CFUs), or mAb- iFt , boosted on day 21 and challenged on day 35 with 10,000 CFUs of Ft LVS. After 48 hours post—LVS challenge, the PECs of immunized mice were harvested and cultured in the presence or absence of Ft . LVS at 1:10 and 1:100 MOI for 24 hrs. The cytokine production was measured as previously described. Results are representative of three independent experiments. (*) P-value
    Immune Complexes Eia Kits, supplied by ALPCO, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Merck KGaA licl immune complex wash buffer
    Administration of <t>mAb-</t> <t>iFt</t> immune complexes reverses the anti-inflammatory properties of LVS in mouse PECs ex vivo . C57BL/6 mice were immunized i.n. with PBS, i Ft (2x10 7 CFUs), or mAb- iFt , boosted on day 21 and challenged on day 35 with 10,000 CFUs of Ft LVS. After 48 hours post—LVS challenge, the PECs of immunized mice were harvested and cultured in the presence or absence of Ft . LVS at 1:10 and 1:100 MOI for 24 hrs. The cytokine production was measured as previously described. Results are representative of three independent experiments. (*) P-value
    Licl Immune Complex Wash Buffer, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Agilent technologies monoclonal pap immune complex
    Administration of <t>mAb-</t> <t>iFt</t> immune complexes reverses the anti-inflammatory properties of LVS in mouse PECs ex vivo . C57BL/6 mice were immunized i.n. with PBS, i Ft (2x10 7 CFUs), or mAb- iFt , boosted on day 21 and challenged on day 35 with 10,000 CFUs of Ft LVS. After 48 hours post—LVS challenge, the PECs of immunized mice were harvested and cultured in the presence or absence of Ft . LVS at 1:10 and 1:100 MOI for 24 hrs. The cytokine production was measured as previously described. Results are representative of three independent experiments. (*) P-value
    Monoclonal Pap Immune Complex, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Zoetis immune complex vaccine bursa plex
    Administration of <t>mAb-</t> <t>iFt</t> immune complexes reverses the anti-inflammatory properties of LVS in mouse PECs ex vivo . C57BL/6 mice were immunized i.n. with PBS, i Ft (2x10 7 CFUs), or mAb- iFt , boosted on day 21 and challenged on day 35 with 10,000 CFUs of Ft LVS. After 48 hours post—LVS challenge, the PECs of immunized mice were harvested and cultured in the presence or absence of Ft . LVS at 1:10 and 1:100 MOI for 24 hrs. The cytokine production was measured as previously described. Results are representative of three independent experiments. (*) P-value
    Immune Complex Vaccine Bursa Plex, supplied by Zoetis, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Plexxikon insoluble immune complexes
    Administration of <t>mAb-</t> <t>iFt</t> immune complexes reverses the anti-inflammatory properties of LVS in mouse PECs ex vivo . C57BL/6 mice were immunized i.n. with PBS, i Ft (2x10 7 CFUs), or mAb- iFt , boosted on day 21 and challenged on day 35 with 10,000 CFUs of Ft LVS. After 48 hours post—LVS challenge, the PECs of immunized mice were harvested and cultured in the presence or absence of Ft . LVS at 1:10 and 1:100 MOI for 24 hrs. The cytokine production was measured as previously described. Results are representative of three independent experiments. (*) P-value
    Insoluble Immune Complexes, supplied by Plexxikon, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Syntaxin 1a immune complexes
    Administration of <t>mAb-</t> <t>iFt</t> immune complexes reverses the anti-inflammatory properties of LVS in mouse PECs ex vivo . C57BL/6 mice were immunized i.n. with PBS, i Ft (2x10 7 CFUs), or mAb- iFt , boosted on day 21 and challenged on day 35 with 10,000 CFUs of Ft LVS. After 48 hours post—LVS challenge, the PECs of immunized mice were harvested and cultured in the presence or absence of Ft . LVS at 1:10 and 1:100 MOI for 24 hrs. The cytokine production was measured as previously described. Results are representative of three independent experiments. (*) P-value
    1a Immune Complexes, supplied by Syntaxin, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Upstate Biotechnology Inc low salt immune complex wash buffer
    Administration of <t>mAb-</t> <t>iFt</t> immune complexes reverses the anti-inflammatory properties of LVS in mouse PECs ex vivo . C57BL/6 mice were immunized i.n. with PBS, i Ft (2x10 7 CFUs), or mAb- iFt , boosted on day 21 and challenged on day 35 with 10,000 CFUs of Ft LVS. After 48 hours post—LVS challenge, the PECs of immunized mice were harvested and cultured in the presence or absence of Ft . LVS at 1:10 and 1:100 MOI for 24 hrs. The cytokine production was measured as previously described. Results are representative of three independent experiments. (*) P-value
    Low Salt Immune Complex Wash Buffer, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 88/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MiraVista Diagnostics immune complex formation
    Administration of <t>mAb-</t> <t>iFt</t> immune complexes reverses the anti-inflammatory properties of LVS in mouse PECs ex vivo . C57BL/6 mice were immunized i.n. with PBS, i Ft (2x10 7 CFUs), or mAb- iFt , boosted on day 21 and challenged on day 35 with 10,000 CFUs of Ft LVS. After 48 hours post—LVS challenge, the PECs of immunized mice were harvested and cultured in the presence or absence of Ft . LVS at 1:10 and 1:100 MOI for 24 hrs. The cytokine production was measured as previously described. Results are representative of three independent experiments. (*) P-value
    Immune Complex Formation, supplied by MiraVista Diagnostics, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Thermo Fisher immune complex stimulated
    Administration of <t>mAb-</t> <t>iFt</t> immune complexes reverses the anti-inflammatory properties of LVS in mouse PECs ex vivo . C57BL/6 mice were immunized i.n. with PBS, i Ft (2x10 7 CFUs), or mAb- iFt , boosted on day 21 and challenged on day 35 with 10,000 CFUs of Ft LVS. After 48 hours post—LVS challenge, the PECs of immunized mice were harvested and cultured in the presence or absence of Ft . LVS at 1:10 and 1:100 MOI for 24 hrs. The cytokine production was measured as previously described. Results are representative of three independent experiments. (*) P-value
    Immune Complex Stimulated, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    IGAN Bio immune complex type
    Administration of <t>mAb-</t> <t>iFt</t> immune complexes reverses the anti-inflammatory properties of LVS in mouse PECs ex vivo . C57BL/6 mice were immunized i.n. with PBS, i Ft (2x10 7 CFUs), or mAb- iFt , boosted on day 21 and challenged on day 35 with 10,000 CFUs of Ft LVS. After 48 hours post—LVS challenge, the PECs of immunized mice were harvested and cultured in the presence or absence of Ft . LVS at 1:10 and 1:100 MOI for 24 hrs. The cytokine production was measured as previously described. Results are representative of three independent experiments. (*) P-value
    Immune Complex Type, supplied by IGAN Bio, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Merck KGaA high salt immune complex wash buffer
    Administration of <t>mAb-</t> <t>iFt</t> immune complexes reverses the anti-inflammatory properties of LVS in mouse PECs ex vivo . C57BL/6 mice were immunized i.n. with PBS, i Ft (2x10 7 CFUs), or mAb- iFt , boosted on day 21 and challenged on day 35 with 10,000 CFUs of Ft LVS. After 48 hours post—LVS challenge, the PECs of immunized mice were harvested and cultured in the presence or absence of Ft . LVS at 1:10 and 1:100 MOI for 24 hrs. The cytokine production was measured as previously described. Results are representative of three independent experiments. (*) P-value
    High Salt Immune Complex Wash Buffer, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Cell Signaling Technology Inc phospho immune complex
    Administration of <t>mAb-</t> <t>iFt</t> immune complexes reverses the anti-inflammatory properties of LVS in mouse PECs ex vivo . C57BL/6 mice were immunized i.n. with PBS, i Ft (2x10 7 CFUs), or mAb- iFt , boosted on day 21 and challenged on day 35 with 10,000 CFUs of Ft LVS. After 48 hours post—LVS challenge, the PECs of immunized mice were harvested and cultured in the presence or absence of Ft . LVS at 1:10 and 1:100 MOI for 24 hrs. The cytokine production was measured as previously described. Results are representative of three independent experiments. (*) P-value
    Phospho Immune Complex, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Validation of subcellular localization predictions using α-HA immunofluorescence The indicated bait proteins fused at their C-terminus with an HA tag were expressed after transient infection of lentiviruses at low multiplicity of infection and after 2 days, cells were fixed and subjected to α-HA-based immunofluorescence (red). Nuclei were stained with Hoechst. For baits with predicted mitochondrial localization, cells were co-stained with α-TOMM20 antibodies (green). Z-series optical sections were acquired via spinning disk confocal microscopy; maximum intensity projections are shown. Scale bar=20 μm.

    Journal: Nature

    Article Title: Architecture of the human interactome defines protein communities and disease networks

    doi: 10.1038/nature22366

    Figure Lengend Snippet: Validation of subcellular localization predictions using α-HA immunofluorescence The indicated bait proteins fused at their C-terminus with an HA tag were expressed after transient infection of lentiviruses at low multiplicity of infection and after 2 days, cells were fixed and subjected to α-HA-based immunofluorescence (red). Nuclei were stained with Hoechst. For baits with predicted mitochondrial localization, cells were co-stained with α-TOMM20 antibodies (green). Z-series optical sections were acquired via spinning disk confocal microscopy; maximum intensity projections are shown. Scale bar=20 μm.

    Article Snippet: Affinity purification was performed essentially as described previously , but eluted α-HA immune complexes (Sigma-Aldrich, clone HA-7) were analyzed in two ways.

    Techniques: Immunofluorescence, Infection, Staining, Confocal Microscopy

    BioPlex network coverage and validation of interactions for a set of poorly studied proteins in BioPlex 2.0 using HCT116 cells a , BioPlex network coverage of selected protein classes. Light shades represent total proteins, while dark shades represent baits targeted for AP-MS. BioPlex 1.0 is depicted in blue shades while BioPlex 2.0 is highlighted in red. b – m , The indicated bait proteins (teal) were expressed in HCT116 cells and α-HA immune complexes analyzed by mass spectrometry. HCIPs were determined using CompPASS-Plus . Interactions observed in both HCT116 and HEK293T cells are indicated with blue edges and nodes. Interactions seen in HEK293T but not HCT116 are shown in grey edges and nodes. b , TMEM111; c , ZNHIT3; d , RMND5A; e , SMTNL2; f , FBXO28; g , C3orf75; h , c9orf41; i , MPP2; j , ZNF219; k , ZNF483; l , WDR37; m , LRCH3.

    Journal: Nature

    Article Title: Architecture of the human interactome defines protein communities and disease networks

    doi: 10.1038/nature22366

    Figure Lengend Snippet: BioPlex network coverage and validation of interactions for a set of poorly studied proteins in BioPlex 2.0 using HCT116 cells a , BioPlex network coverage of selected protein classes. Light shades represent total proteins, while dark shades represent baits targeted for AP-MS. BioPlex 1.0 is depicted in blue shades while BioPlex 2.0 is highlighted in red. b – m , The indicated bait proteins (teal) were expressed in HCT116 cells and α-HA immune complexes analyzed by mass spectrometry. HCIPs were determined using CompPASS-Plus . Interactions observed in both HCT116 and HEK293T cells are indicated with blue edges and nodes. Interactions seen in HEK293T but not HCT116 are shown in grey edges and nodes. b , TMEM111; c , ZNHIT3; d , RMND5A; e , SMTNL2; f , FBXO28; g , C3orf75; h , c9orf41; i , MPP2; j , ZNF219; k , ZNF483; l , WDR37; m , LRCH3.

    Article Snippet: Affinity purification was performed essentially as described previously , but eluted α-HA immune complexes (Sigma-Aldrich, clone HA-7) were analyzed in two ways.

    Techniques: Mass Spectrometry

    Analysis of Fc receptor–mediated macrophage activation and function in WKY, LEW, and WKY. LCrgn1 congenic rats, showing ( A ) Fc oxyburst assay, ( B ) bead phagocytosis assay, ( C ) MCP-1 production after stimulation by LPS, and ( D ) iNOS mRNA expression after stimulation by LPS Results shown represent the mean ± SD for each group. (* P

    Journal: The American Journal of Pathology

    Article Title: Genetic Susceptibility to Experimental Autoimmune Glomerulonephritis in the Wistar Kyoto Rat

    doi: 10.1016/j.ajpath.2012.01.029

    Figure Lengend Snippet: Analysis of Fc receptor–mediated macrophage activation and function in WKY, LEW, and WKY. LCrgn1 congenic rats, showing ( A ) Fc oxyburst assay, ( B ) bead phagocytosis assay, ( C ) MCP-1 production after stimulation by LPS, and ( D ) iNOS mRNA expression after stimulation by LPS Results shown represent the mean ± SD for each group. (* P

    Article Snippet: Briefly, cells (1 × 106 macrophages) were harvested and resuspended in Krebs-Ringer PBS (KRP buffer) with 1.0 mmol/L Ca2+ , 1.5 mmol/L Mg2+ , and 5.5 mmol/L glucose, warmed to 37°C for 10 minutes, then stimulated with the Fc OxyBURST immune complex (120 μg/mL; Invitrogen).

    Techniques: Activation Assay, Phagocytosis Assay, Expressing

    Administration of mAb- iFt immune complexes reverses the anti-inflammatory properties of LVS in mouse PECs ex vivo . C57BL/6 mice were immunized i.n. with PBS, i Ft (2x10 7 CFUs), or mAb- iFt , boosted on day 21 and challenged on day 35 with 10,000 CFUs of Ft LVS. After 48 hours post—LVS challenge, the PECs of immunized mice were harvested and cultured in the presence or absence of Ft . LVS at 1:10 and 1:100 MOI for 24 hrs. The cytokine production was measured as previously described. Results are representative of three independent experiments. (*) P-value

    Journal: PLoS ONE

    Article Title: Targeting of a Fixed Bacterial Immunogen to Fc Receptors Reverses the Anti-Inflammatory Properties of the Gram-Negative Bacterium, Francisella tularensis, during the Early Stages of Infection

    doi: 10.1371/journal.pone.0129981

    Figure Lengend Snippet: Administration of mAb- iFt immune complexes reverses the anti-inflammatory properties of LVS in mouse PECs ex vivo . C57BL/6 mice were immunized i.n. with PBS, i Ft (2x10 7 CFUs), or mAb- iFt , boosted on day 21 and challenged on day 35 with 10,000 CFUs of Ft LVS. After 48 hours post—LVS challenge, the PECs of immunized mice were harvested and cultured in the presence or absence of Ft . LVS at 1:10 and 1:100 MOI for 24 hrs. The cytokine production was measured as previously described. Results are representative of three independent experiments. (*) P-value

    Article Snippet: LPS mAb used to generate mAb-iFt immune complexes was purchased from Fitzgerald (Cat# 10-F02, clone#M023621, Acton, MA).

    Techniques: Ex Vivo, Mouse Assay, Cell Culture