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  • 85
    Millipore soluble antigen
    Soluble Antigen, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/soluble antigen/product/Millipore
    Average 85 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    soluble antigen - by Bioz Stars, 2020-08
    85/100 stars
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    88
    Millipore β 2gp1 antigen
    OD values of C3c (a) bound to anti- <t>β</t> 2GP1 and OD values of anti- β 2GP1-IgM (b) in sera of patients with APS and healthy children. Anti- β 2GP1 of healthy children displayed significantly more bound C3c than that of patients with APS ( p = 0.00007). This suggests a more efficient clearance of β 2GP1-immune complexes in the healthy children. Anti- β 2GP1-IgM OD values of patients with APS were significantly higher than those of healthy children. Horizontal lines represent the median OD value of the cohort. APS = antiphospholipid syndrome; OD = optical density.
    β 2gp1 Antigen, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β 2gp1 antigen/product/Millipore
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    β 2gp1 antigen - by Bioz Stars, 2020-08
    88/100 stars
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    93
    Cocalico antigen
    OD values of C3c (a) bound to anti- <t>β</t> 2GP1 and OD values of anti- β 2GP1-IgM (b) in sera of patients with APS and healthy children. Anti- β 2GP1 of healthy children displayed significantly more bound C3c than that of patients with APS ( p = 0.00007). This suggests a more efficient clearance of β 2GP1-immune complexes in the healthy children. Anti- β 2GP1-IgM OD values of patients with APS were significantly higher than those of healthy children. Horizontal lines represent the median OD value of the cohort. APS = antiphospholipid syndrome; OD = optical density.
    Antigen, supplied by Cocalico, used in various techniques. Bioz Stars score: 93/100, based on 96 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antigen/product/Cocalico
    Average 93 stars, based on 96 article reviews
    Price from $9.99 to $1999.99
    antigen - by Bioz Stars, 2020-08
    93/100 stars
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    92
    Epitomics antigen
    OD values of C3c (a) bound to anti- <t>β</t> 2GP1 and OD values of anti- β 2GP1-IgM (b) in sera of patients with APS and healthy children. Anti- β 2GP1 of healthy children displayed significantly more bound C3c than that of patients with APS ( p = 0.00007). This suggests a more efficient clearance of β 2GP1-immune complexes in the healthy children. Anti- β 2GP1-IgM OD values of patients with APS were significantly higher than those of healthy children. Horizontal lines represent the median OD value of the cohort. APS = antiphospholipid syndrome; OD = optical density.
    Antigen, supplied by Epitomics, used in various techniques. Bioz Stars score: 92/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antigen/product/Epitomics
    Average 92 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    antigen - by Bioz Stars, 2020-08
    92/100 stars
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    92
    Kaneka Corp antigen
    OD values of C3c (a) bound to anti- <t>β</t> 2GP1 and OD values of anti- β 2GP1-IgM (b) in sera of patients with APS and healthy children. Anti- β 2GP1 of healthy children displayed significantly more bound C3c than that of patients with APS ( p = 0.00007). This suggests a more efficient clearance of β 2GP1-immune complexes in the healthy children. Anti- β 2GP1-IgM OD values of patients with APS were significantly higher than those of healthy children. Horizontal lines represent the median OD value of the cohort. APS = antiphospholipid syndrome; OD = optical density.
    Antigen, supplied by Kaneka Corp, used in various techniques. Bioz Stars score: 92/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antigen/product/Kaneka Corp
    Average 92 stars, based on 37 article reviews
    Price from $9.99 to $1999.99
    antigen - by Bioz Stars, 2020-08
    92/100 stars
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    92
    Mine Safety Appliances antigen
    OD values of C3c (a) bound to anti- <t>β</t> 2GP1 and OD values of anti- β 2GP1-IgM (b) in sera of patients with APS and healthy children. Anti- β 2GP1 of healthy children displayed significantly more bound C3c than that of patients with APS ( p = 0.00007). This suggests a more efficient clearance of β 2GP1-immune complexes in the healthy children. Anti- β 2GP1-IgM OD values of patients with APS were significantly higher than those of healthy children. Horizontal lines represent the median OD value of the cohort. APS = antiphospholipid syndrome; OD = optical density.
    Antigen, supplied by Mine Safety Appliances, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antigen/product/Mine Safety Appliances
    Average 92 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    antigen - by Bioz Stars, 2020-08
    92/100 stars
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    92
    Abfrontier antigen
    OD values of C3c (a) bound to anti- <t>β</t> 2GP1 and OD values of anti- β 2GP1-IgM (b) in sera of patients with APS and healthy children. Anti- β 2GP1 of healthy children displayed significantly more bound C3c than that of patients with APS ( p = 0.00007). This suggests a more efficient clearance of β 2GP1-immune complexes in the healthy children. Anti- β 2GP1-IgM OD values of patients with APS were significantly higher than those of healthy children. Horizontal lines represent the median OD value of the cohort. APS = antiphospholipid syndrome; OD = optical density.
    Antigen, supplied by Abfrontier, used in various techniques. Bioz Stars score: 92/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antigen/product/Abfrontier
    Average 92 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    antigen - by Bioz Stars, 2020-08
    92/100 stars
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    92
    GenScript antigen
    OD values of C3c (a) bound to anti- <t>β</t> 2GP1 and OD values of anti- β 2GP1-IgM (b) in sera of patients with APS and healthy children. Anti- β 2GP1 of healthy children displayed significantly more bound C3c than that of patients with APS ( p = 0.00007). This suggests a more efficient clearance of β 2GP1-immune complexes in the healthy children. Anti- β 2GP1-IgM OD values of patients with APS were significantly higher than those of healthy children. Horizontal lines represent the median OD value of the cohort. APS = antiphospholipid syndrome; OD = optical density.
    Antigen, supplied by GenScript, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antigen/product/GenScript
    Average 92 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    antigen - by Bioz Stars, 2020-08
    92/100 stars
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    92
    Agilent technologies antigen
    OD values of C3c (a) bound to anti- <t>β</t> 2GP1 and OD values of anti- β 2GP1-IgM (b) in sera of patients with APS and healthy children. Anti- β 2GP1 of healthy children displayed significantly more bound C3c than that of patients with APS ( p = 0.00007). This suggests a more efficient clearance of β 2GP1-immune complexes in the healthy children. Anti- β 2GP1-IgM OD values of patients with APS were significantly higher than those of healthy children. Horizontal lines represent the median OD value of the cohort. APS = antiphospholipid syndrome; OD = optical density.
    Antigen, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antigen/product/Agilent technologies
    Average 92 stars, based on 65 article reviews
    Price from $9.99 to $1999.99
    antigen - by Bioz Stars, 2020-08
    92/100 stars
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    92
    Alerchek antigen
    OD values of C3c (a) bound to anti- <t>β</t> 2GP1 and OD values of anti- β 2GP1-IgM (b) in sera of patients with APS and healthy children. Anti- β 2GP1 of healthy children displayed significantly more bound C3c than that of patients with APS ( p = 0.00007). This suggests a more efficient clearance of β 2GP1-immune complexes in the healthy children. Anti- β 2GP1-IgM OD values of patients with APS were significantly higher than those of healthy children. Horizontal lines represent the median OD value of the cohort. APS = antiphospholipid syndrome; OD = optical density.
    Antigen, supplied by Alerchek, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antigen/product/Alerchek
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antigen - by Bioz Stars, 2020-08
    92/100 stars
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    92
    Alpha Diagnostics antigen
    OD values of C3c (a) bound to anti- <t>β</t> 2GP1 and OD values of anti- β 2GP1-IgM (b) in sera of patients with APS and healthy children. Anti- β 2GP1 of healthy children displayed significantly more bound C3c than that of patients with APS ( p = 0.00007). This suggests a more efficient clearance of β 2GP1-immune complexes in the healthy children. Anti- β 2GP1-IgM OD values of patients with APS were significantly higher than those of healthy children. Horizontal lines represent the median OD value of the cohort. APS = antiphospholipid syndrome; OD = optical density.
    Antigen, supplied by Alpha Diagnostics, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antigen/product/Alpha Diagnostics
    Average 92 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    antigen - by Bioz Stars, 2020-08
    92/100 stars
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    92
    DYNEX tech antigen
    OD values of C3c (a) bound to anti- <t>β</t> 2GP1 and OD values of anti- β 2GP1-IgM (b) in sera of patients with APS and healthy children. Anti- β 2GP1 of healthy children displayed significantly more bound C3c than that of patients with APS ( p = 0.00007). This suggests a more efficient clearance of β 2GP1-immune complexes in the healthy children. Anti- β 2GP1-IgM OD values of patients with APS were significantly higher than those of healthy children. Horizontal lines represent the median OD value of the cohort. APS = antiphospholipid syndrome; OD = optical density.
    Antigen, supplied by DYNEX tech, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antigen/product/DYNEX tech
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    antigen - by Bioz Stars, 2020-08
    92/100 stars
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    92
    GenWay antigen
    OD values of C3c (a) bound to anti- <t>β</t> 2GP1 and OD values of anti- β 2GP1-IgM (b) in sera of patients with APS and healthy children. Anti- β 2GP1 of healthy children displayed significantly more bound C3c than that of patients with APS ( p = 0.00007). This suggests a more efficient clearance of β 2GP1-immune complexes in the healthy children. Anti- β 2GP1-IgM OD values of patients with APS were significantly higher than those of healthy children. Horizontal lines represent the median OD value of the cohort. APS = antiphospholipid syndrome; OD = optical density.
    Antigen, supplied by GenWay, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antigen/product/GenWay
    Average 92 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    antigen - by Bioz Stars, 2020-08
    92/100 stars
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    92
    R&D Systems antigen
    OD values of C3c (a) bound to anti- <t>β</t> 2GP1 and OD values of anti- β 2GP1-IgM (b) in sera of patients with APS and healthy children. Anti- β 2GP1 of healthy children displayed significantly more bound C3c than that of patients with APS ( p = 0.00007). This suggests a more efficient clearance of β 2GP1-immune complexes in the healthy children. Anti- β 2GP1-IgM OD values of patients with APS were significantly higher than those of healthy children. Horizontal lines represent the median OD value of the cohort. APS = antiphospholipid syndrome; OD = optical density.
    Antigen, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 57 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antigen/product/R&D Systems
    Average 92 stars, based on 57 article reviews
    Price from $9.99 to $1999.99
    antigen - by Bioz Stars, 2020-08
    92/100 stars
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    93
    Santa Cruz Biotechnology antigen
    OD values of C3c (a) bound to anti- <t>β</t> 2GP1 and OD values of anti- β 2GP1-IgM (b) in sera of patients with APS and healthy children. Anti- β 2GP1 of healthy children displayed significantly more bound C3c than that of patients with APS ( p = 0.00007). This suggests a more efficient clearance of β 2GP1-immune complexes in the healthy children. Anti- β 2GP1-IgM OD values of patients with APS were significantly higher than those of healthy children. Horizontal lines represent the median OD value of the cohort. APS = antiphospholipid syndrome; OD = optical density.
    Antigen, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antigen/product/Santa Cruz Biotechnology
    Average 93 stars, based on 112 article reviews
    Price from $9.99 to $1999.99
    antigen - by Bioz Stars, 2020-08
    93/100 stars
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    93
    Thermo Fisher antigen
    OD values of C3c (a) bound to anti- <t>β</t> 2GP1 and OD values of anti- β 2GP1-IgM (b) in sera of patients with APS and healthy children. Anti- β 2GP1 of healthy children displayed significantly more bound C3c than that of patients with APS ( p = 0.00007). This suggests a more efficient clearance of β 2GP1-immune complexes in the healthy children. Anti- β 2GP1-IgM OD values of patients with APS were significantly higher than those of healthy children. Horizontal lines represent the median OD value of the cohort. APS = antiphospholipid syndrome; OD = optical density.
    Antigen, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 223 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antigen/product/Thermo Fisher
    Average 93 stars, based on 223 article reviews
    Price from $9.99 to $1999.99
    antigen - by Bioz Stars, 2020-08
    93/100 stars
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    92
    Virusys antigen
    OD values of C3c (a) bound to anti- <t>β</t> 2GP1 and OD values of anti- β 2GP1-IgM (b) in sera of patients with APS and healthy children. Anti- β 2GP1 of healthy children displayed significantly more bound C3c than that of patients with APS ( p = 0.00007). This suggests a more efficient clearance of β 2GP1-immune complexes in the healthy children. Anti- β 2GP1-IgM OD values of patients with APS were significantly higher than those of healthy children. Horizontal lines represent the median OD value of the cohort. APS = antiphospholipid syndrome; OD = optical density.
    Antigen, supplied by Virusys, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antigen/product/Virusys
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    antigen - by Bioz Stars, 2020-08
    92/100 stars
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    93
    Millipore prest antigen pappa2
    The SPR response curves of the carboxyl-GO-based SPR chip detection immunoassay during <t>PAPPA2</t> and anti-PAPPA2 binding reactions in spiked human plasma. ( A ) The SPR sensorgrams of non-specific interference in different dilutions of human plasma. ( B ) Comparison of the efficiency of different concentrations of anti-PAPPA2 with PAPPA2 interactions. ( C ) Calibration curve of the average SPR response to various PAPPA2 protein concentrations. ( D ) Molecular binding affinity and kinetic analysis of the interaction factors. Abbreviations: SPR, surface plasmon resonance; PAPPA2, pregnancy-associated plasma protein A2; Carboxyl-GO, carboxyl-graphene oxide; K D , dissociation constant; k a , association rate.
    Prest Antigen Pappa2, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prest antigen pappa2/product/Millipore
    Average 93 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    prest antigen pappa2 - by Bioz Stars, 2020-08
    93/100 stars
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    85
    Millipore srgap2 antigen
    PAX-captured proteins are MS identifiable. ( A ) PAX captures a known interacting protein following over-expression in FreeStyle 293 cells. An array of peptides consisting of a proline-rich sequence of FMNL1 and a control sequence were incubated with FreeStyle 293 cell lysate overexpressing <t>srGAP2,</t> photo-crosslinked and washed at high stringency. Immunoblotting with srGAP2 antibody detected phototrapping of srGAP2 by PAX. pBpa in the bait peptides is indicated in blue. ( B ) Comparative immunoblotting of srGAP2 antigen dot-blot. Different amounts of srGAP2 were spotted onto nitrocellulose membrane and simultaneously immunoblotted with srGAP2 antibody. ( C ) Identification of srGAP2 by mass spectrometry. The five spots with the highest amount of crosslinked srGAP2 from a duplicate blot of (A) were subjected to MS/MS. 3 unique peptides (underlined) covering 8% of the entire sequence of srGAP2 protein were identified. ( D ) PAX captures a known interacting protein from mouse brain lysate. Peptide array comprising mGluR5 sequences with a PPXXF motif was incubated with mouse brain lysate, photo-crosslinked and subjected to high stringency washes. Immunoblotting with Homer1 antibody indicates photo-trapping of Homer1 by PAX. pBpa in the bait peptides is indicated in blue.
    Srgap2 Antigen, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/srgap2 antigen/product/Millipore
    Average 85 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    srgap2 antigen - by Bioz Stars, 2020-08
    85/100 stars
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    90
    JPT Peptide Technologies GmbH antigen
    PAX-captured proteins are MS identifiable. ( A ) PAX captures a known interacting protein following over-expression in FreeStyle 293 cells. An array of peptides consisting of a proline-rich sequence of FMNL1 and a control sequence were incubated with FreeStyle 293 cell lysate overexpressing <t>srGAP2,</t> photo-crosslinked and washed at high stringency. Immunoblotting with srGAP2 antibody detected phototrapping of srGAP2 by PAX. pBpa in the bait peptides is indicated in blue. ( B ) Comparative immunoblotting of srGAP2 antigen dot-blot. Different amounts of srGAP2 were spotted onto nitrocellulose membrane and simultaneously immunoblotted with srGAP2 antibody. ( C ) Identification of srGAP2 by mass spectrometry. The five spots with the highest amount of crosslinked srGAP2 from a duplicate blot of (A) were subjected to MS/MS. 3 unique peptides (underlined) covering 8% of the entire sequence of srGAP2 protein were identified. ( D ) PAX captures a known interacting protein from mouse brain lysate. Peptide array comprising mGluR5 sequences with a PPXXF motif was incubated with mouse brain lysate, photo-crosslinked and subjected to high stringency washes. Immunoblotting with Homer1 antibody indicates photo-trapping of Homer1 by PAX. pBpa in the bait peptides is indicated in blue.
    Antigen, supplied by JPT Peptide Technologies GmbH, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antigen/product/JPT Peptide Technologies GmbH
    Average 90 stars, based on 2 article reviews
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    antigen - by Bioz Stars, 2020-08
    90/100 stars
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    92
    Biocon antigen
    PAX-captured proteins are MS identifiable. ( A ) PAX captures a known interacting protein following over-expression in FreeStyle 293 cells. An array of peptides consisting of a proline-rich sequence of FMNL1 and a control sequence were incubated with FreeStyle 293 cell lysate overexpressing <t>srGAP2,</t> photo-crosslinked and washed at high stringency. Immunoblotting with srGAP2 antibody detected phototrapping of srGAP2 by PAX. pBpa in the bait peptides is indicated in blue. ( B ) Comparative immunoblotting of srGAP2 antigen dot-blot. Different amounts of srGAP2 were spotted onto nitrocellulose membrane and simultaneously immunoblotted with srGAP2 antibody. ( C ) Identification of srGAP2 by mass spectrometry. The five spots with the highest amount of crosslinked srGAP2 from a duplicate blot of (A) were subjected to MS/MS. 3 unique peptides (underlined) covering 8% of the entire sequence of srGAP2 protein were identified. ( D ) PAX captures a known interacting protein from mouse brain lysate. Peptide array comprising mGluR5 sequences with a PPXXF motif was incubated with mouse brain lysate, photo-crosslinked and subjected to high stringency washes. Immunoblotting with Homer1 antibody indicates photo-trapping of Homer1 by PAX. pBpa in the bait peptides is indicated in blue.
    Antigen, supplied by Biocon, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antigen/product/Biocon
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    antigen - by Bioz Stars, 2020-08
    92/100 stars
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    92
    BioLegend antigen
    PAX-captured proteins are MS identifiable. ( A ) PAX captures a known interacting protein following over-expression in FreeStyle 293 cells. An array of peptides consisting of a proline-rich sequence of FMNL1 and a control sequence were incubated with FreeStyle 293 cell lysate overexpressing <t>srGAP2,</t> photo-crosslinked and washed at high stringency. Immunoblotting with srGAP2 antibody detected phototrapping of srGAP2 by PAX. pBpa in the bait peptides is indicated in blue. ( B ) Comparative immunoblotting of srGAP2 antigen dot-blot. Different amounts of srGAP2 were spotted onto nitrocellulose membrane and simultaneously immunoblotted with srGAP2 antibody. ( C ) Identification of srGAP2 by mass spectrometry. The five spots with the highest amount of crosslinked srGAP2 from a duplicate blot of (A) were subjected to MS/MS. 3 unique peptides (underlined) covering 8% of the entire sequence of srGAP2 protein were identified. ( D ) PAX captures a known interacting protein from mouse brain lysate. Peptide array comprising mGluR5 sequences with a PPXXF motif was incubated with mouse brain lysate, photo-crosslinked and subjected to high stringency washes. Immunoblotting with Homer1 antibody indicates photo-trapping of Homer1 by PAX. pBpa in the bait peptides is indicated in blue.
    Antigen, supplied by BioLegend, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antigen/product/BioLegend
    Average 92 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    antigen - by Bioz Stars, 2020-08
    92/100 stars
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    92
    EUROIMMUN antigen
    PAX-captured proteins are MS identifiable. ( A ) PAX captures a known interacting protein following over-expression in FreeStyle 293 cells. An array of peptides consisting of a proline-rich sequence of FMNL1 and a control sequence were incubated with FreeStyle 293 cell lysate overexpressing <t>srGAP2,</t> photo-crosslinked and washed at high stringency. Immunoblotting with srGAP2 antibody detected phototrapping of srGAP2 by PAX. pBpa in the bait peptides is indicated in blue. ( B ) Comparative immunoblotting of srGAP2 antigen dot-blot. Different amounts of srGAP2 were spotted onto nitrocellulose membrane and simultaneously immunoblotted with srGAP2 antibody. ( C ) Identification of srGAP2 by mass spectrometry. The five spots with the highest amount of crosslinked srGAP2 from a duplicate blot of (A) were subjected to MS/MS. 3 unique peptides (underlined) covering 8% of the entire sequence of srGAP2 protein were identified. ( D ) PAX captures a known interacting protein from mouse brain lysate. Peptide array comprising mGluR5 sequences with a PPXXF motif was incubated with mouse brain lysate, photo-crosslinked and subjected to high stringency washes. Immunoblotting with Homer1 antibody indicates photo-trapping of Homer1 by PAX. pBpa in the bait peptides is indicated in blue.
    Antigen, supplied by EUROIMMUN, used in various techniques. Bioz Stars score: 92/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antigen/product/EUROIMMUN
    Average 92 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    antigen - by Bioz Stars, 2020-08
    92/100 stars
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    92
    Lifespan Biosciences antigen
    PAX-captured proteins are MS identifiable. ( A ) PAX captures a known interacting protein following over-expression in FreeStyle 293 cells. An array of peptides consisting of a proline-rich sequence of FMNL1 and a control sequence were incubated with FreeStyle 293 cell lysate overexpressing <t>srGAP2,</t> photo-crosslinked and washed at high stringency. Immunoblotting with srGAP2 antibody detected phototrapping of srGAP2 by PAX. pBpa in the bait peptides is indicated in blue. ( B ) Comparative immunoblotting of srGAP2 antigen dot-blot. Different amounts of srGAP2 were spotted onto nitrocellulose membrane and simultaneously immunoblotted with srGAP2 antibody. ( C ) Identification of srGAP2 by mass spectrometry. The five spots with the highest amount of crosslinked srGAP2 from a duplicate blot of (A) were subjected to MS/MS. 3 unique peptides (underlined) covering 8% of the entire sequence of srGAP2 protein were identified. ( D ) PAX captures a known interacting protein from mouse brain lysate. Peptide array comprising mGluR5 sequences with a PPXXF motif was incubated with mouse brain lysate, photo-crosslinked and subjected to high stringency washes. Immunoblotting with Homer1 antibody indicates photo-trapping of Homer1 by PAX. pBpa in the bait peptides is indicated in blue.
    Antigen, supplied by Lifespan Biosciences, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antigen/product/Lifespan Biosciences
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    antigen - by Bioz Stars, 2020-08
    92/100 stars
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    91
    MyBiosource antigen
    PAX-captured proteins are MS identifiable. ( A ) PAX captures a known interacting protein following over-expression in FreeStyle 293 cells. An array of peptides consisting of a proline-rich sequence of FMNL1 and a control sequence were incubated with FreeStyle 293 cell lysate overexpressing <t>srGAP2,</t> photo-crosslinked and washed at high stringency. Immunoblotting with srGAP2 antibody detected phototrapping of srGAP2 by PAX. pBpa in the bait peptides is indicated in blue. ( B ) Comparative immunoblotting of srGAP2 antigen dot-blot. Different amounts of srGAP2 were spotted onto nitrocellulose membrane and simultaneously immunoblotted with srGAP2 antibody. ( C ) Identification of srGAP2 by mass spectrometry. The five spots with the highest amount of crosslinked srGAP2 from a duplicate blot of (A) were subjected to MS/MS. 3 unique peptides (underlined) covering 8% of the entire sequence of srGAP2 protein were identified. ( D ) PAX captures a known interacting protein from mouse brain lysate. Peptide array comprising mGluR5 sequences with a PPXXF motif was incubated with mouse brain lysate, photo-crosslinked and subjected to high stringency washes. Immunoblotting with Homer1 antibody indicates photo-trapping of Homer1 by PAX. pBpa in the bait peptides is indicated in blue.
    Antigen, supplied by MyBiosource, used in various techniques. Bioz Stars score: 91/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    OD values of C3c (a) bound to anti- β 2GP1 and OD values of anti- β 2GP1-IgM (b) in sera of patients with APS and healthy children. Anti- β 2GP1 of healthy children displayed significantly more bound C3c than that of patients with APS ( p = 0.00007). This suggests a more efficient clearance of β 2GP1-immune complexes in the healthy children. Anti- β 2GP1-IgM OD values of patients with APS were significantly higher than those of healthy children. Horizontal lines represent the median OD value of the cohort. APS = antiphospholipid syndrome; OD = optical density.

    Journal: Journal of Immunology Research

    Article Title: The Pathogenicity of Anti-β2GP1-IgG Autoantibodies Depends on Fc Glycosylation

    doi: 10.1155/2015/638129

    Figure Lengend Snippet: OD values of C3c (a) bound to anti- β 2GP1 and OD values of anti- β 2GP1-IgM (b) in sera of patients with APS and healthy children. Anti- β 2GP1 of healthy children displayed significantly more bound C3c than that of patients with APS ( p = 0.00007). This suggests a more efficient clearance of β 2GP1-immune complexes in the healthy children. Anti- β 2GP1-IgM OD values of patients with APS were significantly higher than those of healthy children. Horizontal lines represent the median OD value of the cohort. APS = antiphospholipid syndrome; OD = optical density.

    Article Snippet: Before coating, the β 2GP1 antigen was treated with 1% of periodic acid (PIA, Sigma-Aldrich, Steinheim, Germany) for 48 hours at 43°C to remove its sugar residues to exclude unspecific binding of Sambucus nigra lectin (SNA) to naive β 2GP1.

    Techniques:

    SNA/anti- β 2GP1-IgG ratio in PAPS, SAPS, SLE + aPL, aaPL, and children. Healthy children show a significantly higher SNA/anti- β 2GP1-IgG ratio than PAPS, SAPS, SLE + aPL, and aaPL ( ∗∗ p

    Journal: Journal of Immunology Research

    Article Title: The Pathogenicity of Anti-β2GP1-IgG Autoantibodies Depends on Fc Glycosylation

    doi: 10.1155/2015/638129

    Figure Lengend Snippet: SNA/anti- β 2GP1-IgG ratio in PAPS, SAPS, SLE + aPL, aaPL, and children. Healthy children show a significantly higher SNA/anti- β 2GP1-IgG ratio than PAPS, SAPS, SLE + aPL, and aaPL ( ∗∗ p

    Article Snippet: Before coating, the β 2GP1 antigen was treated with 1% of periodic acid (PIA, Sigma-Aldrich, Steinheim, Germany) for 48 hours at 43°C to remove its sugar residues to exclude unspecific binding of Sambucus nigra lectin (SNA) to naive β 2GP1.

    Techniques: Papanicolaou Stain

    Total anti- β 2GP1 IgG in the sera of NHD, PAPS, SLE/APS, and healthy children and the corresponding p values. NHD showed significantly lower mOD values than all other cohorts whereas healthy children represented the highest mOD values. Short horizontal lines: mean mOD values of all samples of the cohort. Long broken line: 97.5% quantile of NHD (=738 mOD); NHD = normal healthy donor; PAPS = primary antiphospholipid syndrome; SLE/APS = antiphospholipid syndrome with systemic lupus erythematosus; OD = optical density.

    Journal: Journal of Immunology Research

    Article Title: The Pathogenicity of Anti-β2GP1-IgG Autoantibodies Depends on Fc Glycosylation

    doi: 10.1155/2015/638129

    Figure Lengend Snippet: Total anti- β 2GP1 IgG in the sera of NHD, PAPS, SLE/APS, and healthy children and the corresponding p values. NHD showed significantly lower mOD values than all other cohorts whereas healthy children represented the highest mOD values. Short horizontal lines: mean mOD values of all samples of the cohort. Long broken line: 97.5% quantile of NHD (=738 mOD); NHD = normal healthy donor; PAPS = primary antiphospholipid syndrome; SLE/APS = antiphospholipid syndrome with systemic lupus erythematosus; OD = optical density.

    Article Snippet: Before coating, the β 2GP1 antigen was treated with 1% of periodic acid (PIA, Sigma-Aldrich, Steinheim, Germany) for 48 hours at 43°C to remove its sugar residues to exclude unspecific binding of Sambucus nigra lectin (SNA) to naive β 2GP1.

    Techniques: Papanicolaou Stain

    mOD values of anti- β 2GP1-IgG1 (a), anti- β 2GP1-IgG2 (b), anti- β 2GP1-IgG3 (c), and anti- β 2GP1-IgG4 (d) in NHD, PAPS, SLE/APS, and children. The horizontal lines represent the cut-off for seropositivity of the corresponding anti- β 2GP IgG-subclass ( > mean mOD [NHD]  + 2 ×  SD [NHD] ); NHD = normal healthy donor; PAPS = primary antiphospholipid syndrome; SLE/APS = antiphospholipid syndrome with systemic lupus erythematosus; OD = optical density. Median OD values of the anti- β 2GP1-IgG subclasses in the study cohorts (e). Regarding the anti- β 2GP1-IgG subclass-distribution within the cohorts, children showed a significantly higher contribution of IgG3 than IgG1 and IgG2. Patients with antiphospholipid syndrome (APS) showed a higher content of anti- β 2GP1-IgG2 than -IgG1 and -IgG3. NHD showed a higher contribution of anti- β 2GP1-IgG2 than -IgG1, -IgG3, and -IgG4. The columns represent the median OD values of IgG1, IgG2, IgG3, and IgG4 in the cohorts. OD = optical density; NHD = normal healthy donor. Percentage distribution of anti- β 2GP1-IgG subclasses in children and patients with APS (f). Children harbour significantly less anti- β 2GP1-IgG2 than patients with APS but more anti- β 2GP1-IgG3. APS = antiphospholipid syndrome.

    Journal: Journal of Immunology Research

    Article Title: The Pathogenicity of Anti-β2GP1-IgG Autoantibodies Depends on Fc Glycosylation

    doi: 10.1155/2015/638129

    Figure Lengend Snippet: mOD values of anti- β 2GP1-IgG1 (a), anti- β 2GP1-IgG2 (b), anti- β 2GP1-IgG3 (c), and anti- β 2GP1-IgG4 (d) in NHD, PAPS, SLE/APS, and children. The horizontal lines represent the cut-off for seropositivity of the corresponding anti- β 2GP IgG-subclass ( > mean mOD [NHD] + 2 ×  SD [NHD] ); NHD = normal healthy donor; PAPS = primary antiphospholipid syndrome; SLE/APS = antiphospholipid syndrome with systemic lupus erythematosus; OD = optical density. Median OD values of the anti- β 2GP1-IgG subclasses in the study cohorts (e). Regarding the anti- β 2GP1-IgG subclass-distribution within the cohorts, children showed a significantly higher contribution of IgG3 than IgG1 and IgG2. Patients with antiphospholipid syndrome (APS) showed a higher content of anti- β 2GP1-IgG2 than -IgG1 and -IgG3. NHD showed a higher contribution of anti- β 2GP1-IgG2 than -IgG1, -IgG3, and -IgG4. The columns represent the median OD values of IgG1, IgG2, IgG3, and IgG4 in the cohorts. OD = optical density; NHD = normal healthy donor. Percentage distribution of anti- β 2GP1-IgG subclasses in children and patients with APS (f). Children harbour significantly less anti- β 2GP1-IgG2 than patients with APS but more anti- β 2GP1-IgG3. APS = antiphospholipid syndrome.

    Article Snippet: Before coating, the β 2GP1 antigen was treated with 1% of periodic acid (PIA, Sigma-Aldrich, Steinheim, Germany) for 48 hours at 43°C to remove its sugar residues to exclude unspecific binding of Sambucus nigra lectin (SNA) to naive β 2GP1.

    Techniques: Papanicolaou Stain

    The SPR response curves of the carboxyl-GO-based SPR chip detection immunoassay during PAPPA2 and anti-PAPPA2 binding reactions in spiked human plasma. ( A ) The SPR sensorgrams of non-specific interference in different dilutions of human plasma. ( B ) Comparison of the efficiency of different concentrations of anti-PAPPA2 with PAPPA2 interactions. ( C ) Calibration curve of the average SPR response to various PAPPA2 protein concentrations. ( D ) Molecular binding affinity and kinetic analysis of the interaction factors. Abbreviations: SPR, surface plasmon resonance; PAPPA2, pregnancy-associated plasma protein A2; Carboxyl-GO, carboxyl-graphene oxide; K D , dissociation constant; k a , association rate.

    Journal: International Journal of Nanomedicine

    Article Title: Development of a bioaffinity SPR immunosensor based on functionalized graphene oxide for the detection of pregnancy-associated plasma protein A2 in human plasma

    doi: 10.2147/IJN.S213653

    Figure Lengend Snippet: The SPR response curves of the carboxyl-GO-based SPR chip detection immunoassay during PAPPA2 and anti-PAPPA2 binding reactions in spiked human plasma. ( A ) The SPR sensorgrams of non-specific interference in different dilutions of human plasma. ( B ) Comparison of the efficiency of different concentrations of anti-PAPPA2 with PAPPA2 interactions. ( C ) Calibration curve of the average SPR response to various PAPPA2 protein concentrations. ( D ) Molecular binding affinity and kinetic analysis of the interaction factors. Abbreviations: SPR, surface plasmon resonance; PAPPA2, pregnancy-associated plasma protein A2; Carboxyl-GO, carboxyl-graphene oxide; K D , dissociation constant; k a , association rate.

    Article Snippet: Preparation of the carboxyl-go-based SPR chip to detect PAPPA2 protein in spiked human plasma When detecting antigen PAPPA2 (molecular weight of approximately 27 kDa, APREST73992, Sigma-Aldrich, USA) protein in spiked human plasma, it is important to consider SPR angle errors and deviations caused by non-specific and free protein interference.

    Techniques: SPR Assay, Chromatin Immunoprecipitation, Binding Assay

    Analysis of the sensing material surface chemical structure with regards to FTIR spectra and zeta potential properties. ( A ) Comparison of FTIR spectra of covalent bonds between the GO and carboxyl-GO with anti-PAPPA2 proteins. ( B ) Comparison of zeta potential of GO and carboxyl-GO sheets dispersed in water and graphene sheets dispersed in ethanol solvent. Abbreviations: FTIR, Fourier transform infrared spectroscopy; GO, graphene oxide; carboxyl-GO, carboxyl-graphene oxide; PAPPA2, pregnancy-associated plasma protein A2.

    Journal: International Journal of Nanomedicine

    Article Title: Development of a bioaffinity SPR immunosensor based on functionalized graphene oxide for the detection of pregnancy-associated plasma protein A2 in human plasma

    doi: 10.2147/IJN.S213653

    Figure Lengend Snippet: Analysis of the sensing material surface chemical structure with regards to FTIR spectra and zeta potential properties. ( A ) Comparison of FTIR spectra of covalent bonds between the GO and carboxyl-GO with anti-PAPPA2 proteins. ( B ) Comparison of zeta potential of GO and carboxyl-GO sheets dispersed in water and graphene sheets dispersed in ethanol solvent. Abbreviations: FTIR, Fourier transform infrared spectroscopy; GO, graphene oxide; carboxyl-GO, carboxyl-graphene oxide; PAPPA2, pregnancy-associated plasma protein A2.

    Article Snippet: Preparation of the carboxyl-go-based SPR chip to detect PAPPA2 protein in spiked human plasma When detecting antigen PAPPA2 (molecular weight of approximately 27 kDa, APREST73992, Sigma-Aldrich, USA) protein in spiked human plasma, it is important to consider SPR angle errors and deviations caused by non-specific and free protein interference.

    Techniques: Spectroscopy

    Quantitative analysis of the SPR sensorgram revealed the immune responses to anti-PAPPA2 proteins at different concentrations. ( A ) Sensorgram of different concentrations of PAPPA2 antibodies. ( B ) Binding affinity and kinetic analysis of the immune response interactions of the dissociation constant (K D ) and association rate (k a ). ( C ) Plots of SPR angle shift versus five different interfering proteins and PAPPA2. The assay had high specificity with PAPPA2 against bio-interferences. ( D ) Calibration curves for the detection of PAPPA2 concentrations and the five spiked interfering proteins. Abbreviations: SPR, surface plasmon resonance; PAPPA2, pregnancy-associated plasma protein A2.

    Journal: International Journal of Nanomedicine

    Article Title: Development of a bioaffinity SPR immunosensor based on functionalized graphene oxide for the detection of pregnancy-associated plasma protein A2 in human plasma

    doi: 10.2147/IJN.S213653

    Figure Lengend Snippet: Quantitative analysis of the SPR sensorgram revealed the immune responses to anti-PAPPA2 proteins at different concentrations. ( A ) Sensorgram of different concentrations of PAPPA2 antibodies. ( B ) Binding affinity and kinetic analysis of the immune response interactions of the dissociation constant (K D ) and association rate (k a ). ( C ) Plots of SPR angle shift versus five different interfering proteins and PAPPA2. The assay had high specificity with PAPPA2 against bio-interferences. ( D ) Calibration curves for the detection of PAPPA2 concentrations and the five spiked interfering proteins. Abbreviations: SPR, surface plasmon resonance; PAPPA2, pregnancy-associated plasma protein A2.

    Article Snippet: Preparation of the carboxyl-go-based SPR chip to detect PAPPA2 protein in spiked human plasma When detecting antigen PAPPA2 (molecular weight of approximately 27 kDa, APREST73992, Sigma-Aldrich, USA) protein in spiked human plasma, it is important to consider SPR angle errors and deviations caused by non-specific and free protein interference.

    Techniques: SPR Assay, Binding Assay

    PAX-captured proteins are MS identifiable. ( A ) PAX captures a known interacting protein following over-expression in FreeStyle 293 cells. An array of peptides consisting of a proline-rich sequence of FMNL1 and a control sequence were incubated with FreeStyle 293 cell lysate overexpressing srGAP2, photo-crosslinked and washed at high stringency. Immunoblotting with srGAP2 antibody detected phototrapping of srGAP2 by PAX. pBpa in the bait peptides is indicated in blue. ( B ) Comparative immunoblotting of srGAP2 antigen dot-blot. Different amounts of srGAP2 were spotted onto nitrocellulose membrane and simultaneously immunoblotted with srGAP2 antibody. ( C ) Identification of srGAP2 by mass spectrometry. The five spots with the highest amount of crosslinked srGAP2 from a duplicate blot of (A) were subjected to MS/MS. 3 unique peptides (underlined) covering 8% of the entire sequence of srGAP2 protein were identified. ( D ) PAX captures a known interacting protein from mouse brain lysate. Peptide array comprising mGluR5 sequences with a PPXXF motif was incubated with mouse brain lysate, photo-crosslinked and subjected to high stringency washes. Immunoblotting with Homer1 antibody indicates photo-trapping of Homer1 by PAX. pBpa in the bait peptides is indicated in blue.

    Journal: PLoS ONE

    Article Title: Peptide Array X-Linking (PAX): A New Peptide-Protein Identification Approach

    doi: 10.1371/journal.pone.0037035

    Figure Lengend Snippet: PAX-captured proteins are MS identifiable. ( A ) PAX captures a known interacting protein following over-expression in FreeStyle 293 cells. An array of peptides consisting of a proline-rich sequence of FMNL1 and a control sequence were incubated with FreeStyle 293 cell lysate overexpressing srGAP2, photo-crosslinked and washed at high stringency. Immunoblotting with srGAP2 antibody detected phototrapping of srGAP2 by PAX. pBpa in the bait peptides is indicated in blue. ( B ) Comparative immunoblotting of srGAP2 antigen dot-blot. Different amounts of srGAP2 were spotted onto nitrocellulose membrane and simultaneously immunoblotted with srGAP2 antibody. ( C ) Identification of srGAP2 by mass spectrometry. The five spots with the highest amount of crosslinked srGAP2 from a duplicate blot of (A) were subjected to MS/MS. 3 unique peptides (underlined) covering 8% of the entire sequence of srGAP2 protein were identified. ( D ) PAX captures a known interacting protein from mouse brain lysate. Peptide array comprising mGluR5 sequences with a PPXXF motif was incubated with mouse brain lysate, photo-crosslinked and subjected to high stringency washes. Immunoblotting with Homer1 antibody indicates photo-trapping of Homer1 by PAX. pBpa in the bait peptides is indicated in blue.

    Article Snippet: The dot blot of srGAP2 antigen was made using S & S Manifold I dot-blot array system (Sigma-Aldrich).

    Techniques: Mass Spectrometry, Over Expression, Sequencing, Incubation, Dot Blot, Peptide Microarray