Journal: Journal of Immunology Research
Article Title: The Pathogenicity of Anti-β2GP1-IgG Autoantibodies Depends on Fc Glycosylation
Figure Lengend Snippet: mOD values of anti- β 2GP1-IgG1 (a), anti- β 2GP1-IgG2 (b), anti- β 2GP1-IgG3 (c), and anti- β 2GP1-IgG4 (d) in NHD, PAPS, SLE/APS, and children. The horizontal lines represent the cut-off for seropositivity of the corresponding anti- β 2GP IgG-subclass ( > mean mOD [NHD] + 2 × SD [NHD] ); NHD = normal healthy donor; PAPS = primary antiphospholipid syndrome; SLE/APS = antiphospholipid syndrome with systemic lupus erythematosus; OD = optical density. Median OD values of the anti- β 2GP1-IgG subclasses in the study cohorts (e). Regarding the anti- β 2GP1-IgG subclass-distribution within the cohorts, children showed a significantly higher contribution of IgG3 than IgG1 and IgG2. Patients with antiphospholipid syndrome (APS) showed a higher content of anti- β 2GP1-IgG2 than -IgG1 and -IgG3. NHD showed a higher contribution of anti- β 2GP1-IgG2 than -IgG1, -IgG3, and -IgG4. The columns represent the median OD values of IgG1, IgG2, IgG3, and IgG4 in the cohorts. OD = optical density; NHD = normal healthy donor. Percentage distribution of anti- β 2GP1-IgG subclasses in children and patients with APS (f). Children harbour significantly less anti- β 2GP1-IgG2 than patients with APS but more anti- β 2GP1-IgG3. APS = antiphospholipid syndrome.
Article Snippet: Before coating, the β 2GP1 antigen was treated with 1% of periodic acid (PIA, Sigma-Aldrich, Steinheim, Germany) for 48 hours at 43°C to remove its sugar residues to exclude unspecific binding of Sambucus nigra lectin (SNA) to naive β 2GP1.
Techniques: Papanicolaou Stain