antibody against ripk3 Search Results


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  • 86
    Proteintech antibodies against ripk3
    TRAF5 knockdown enhances necroptosis and attenuates the proliferation of VSMCs induced by oxidized-low density lipoprotein (ox-LDL). A RT-qPCR to confirming TRAF5 expression in VSMCs treated with TRAF5 siRNAs. B - E Western blotting detecting the levels of <t>RIPK3,</t> MLKL, and p-MLKL in ox-LDL-induced VSMCs with TRAF5 knockdown ( n = 3, normalized to GAPDH). F Cell counting kit 8 assay (CCK8) evaluating cell proliferation ( n = 3). G , H EdU assay evaluating cell proliferation ( n = 3). I , J Necroptosis was detected by flow cytometry after Annexin V/PI staining. Annexin V-/PI- represents living cells, Annexin V + /PI- represents early necroptotic cells, and Annexin V + /PI + represents necroptotic cells ( n = 3). (ns: no significance; * p < 0.05; ** p < 0.01; *** p < 0.001)
    Antibodies Against Ripk3, supplied by Proteintech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against ripk3/product/Proteintech
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against ripk3 - by Bioz Stars, 2024-07
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    86
    Cell Signaling Technology Inc antibodies against ripk3
    Adjuvant chemotherapy regimen in <t> RIPK3 </t> protein high- and low- expression groups
    Antibodies Against Ripk3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against ripk3/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against ripk3 - by Bioz Stars, 2024-07
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    86
    Abcam antibodies against ripk3
    Immunofluorescence for <t>RIPK3.</t> (A) Immunofluorescence staining of RIPK3 in the cerebral cortex at 24 h after CA/CPR. Scale bar: 20 μm. The light color granules detected were positive cells. Scale bar (White short bar): 20 μm. (B) RIPK3-positive rate (RIPK3-positive area/DAPI-positive area) per group. All data are mean ± SEM. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. sham; ## p < 0.01 vs. CA; and & p < 0.05 vs. Gly. RIPK3: receptor-interacting serine/threonine kinase 3; CA: cardiac arrest/0.9% saline group; CPR: cardiopulmonary resuscitation; DAPI: 4′,6-diamidino-2-phenylindole; SEM: standard error of the mean; sham: sham-operated group; Gly: 10% glycerol group.
    Antibodies Against Ripk3, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against ripk3/product/Abcam
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against ripk3 - by Bioz Stars, 2024-07
    86/100 stars
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    86
    Genentech inc antibody against ripk3
    Immunofluorescence for <t>RIPK3.</t> (A) Immunofluorescence staining of RIPK3 in the cerebral cortex at 24 h after CA/CPR. Scale bar: 20 μm. The light color granules detected were positive cells. Scale bar (White short bar): 20 μm. (B) RIPK3-positive rate (RIPK3-positive area/DAPI-positive area) per group. All data are mean ± SEM. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. sham; ## p < 0.01 vs. CA; and & p < 0.05 vs. Gly. RIPK3: receptor-interacting serine/threonine kinase 3; CA: cardiac arrest/0.9% saline group; CPR: cardiopulmonary resuscitation; DAPI: 4′,6-diamidino-2-phenylindole; SEM: standard error of the mean; sham: sham-operated group; Gly: 10% glycerol group.
    Antibody Against Ripk3, supplied by Genentech inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody against ripk3/product/Genentech inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibody against ripk3 - by Bioz Stars, 2024-07
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    86
    WuXi AppTec antibodies against ripk3
    STING -/- mice display increased necroptosis during early S.aureus induced pneumonia. WT and STING -/- mice were infected intranasally with S.aureus (1 × 10 8 CFU/mouse) and then were euthanized at 6 hpi. (A, B) TUNEL staining of dead cells in the lung tissues. (C) LDH released in BALF. (D, E) The numbers of neutrophils and macrophages in the BALF were enumerated. (F, G) Propidium iodide positive (PI + ) macrophages in the BALF were analyzed by flow cytometry. (H) The lung tissue lysate was analyzed for p-MLKL, MLKL, and <t>RIPK3</t> by western blotting. GAPDH was used as a loading control. Student’s t-test was performed. Statistical significance is indicated by *p < 0.05 and **p < 0.01.
    Antibodies Against Ripk3, supplied by WuXi AppTec, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against ripk3/product/WuXi AppTec
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against ripk3 - by Bioz Stars, 2024-07
    86/100 stars
      Buy from Supplier

    Image Search Results


    TRAF5 knockdown enhances necroptosis and attenuates the proliferation of VSMCs induced by oxidized-low density lipoprotein (ox-LDL). A RT-qPCR to confirming TRAF5 expression in VSMCs treated with TRAF5 siRNAs. B - E Western blotting detecting the levels of RIPK3, MLKL, and p-MLKL in ox-LDL-induced VSMCs with TRAF5 knockdown ( n = 3, normalized to GAPDH). F Cell counting kit 8 assay (CCK8) evaluating cell proliferation ( n = 3). G , H EdU assay evaluating cell proliferation ( n = 3). I , J Necroptosis was detected by flow cytometry after Annexin V/PI staining. Annexin V-/PI- represents living cells, Annexin V + /PI- represents early necroptotic cells, and Annexin V + /PI + represents necroptotic cells ( n = 3). (ns: no significance; * p < 0.05; ** p < 0.01; *** p < 0.001)

    Journal: BMC Medical Genomics

    Article Title: Identification of necroptosis-related gene TRAF5 as potential target of diagnosing atherosclerosis and assessing its stability

    doi: 10.1186/s12920-023-01573-0

    Figure Lengend Snippet: TRAF5 knockdown enhances necroptosis and attenuates the proliferation of VSMCs induced by oxidized-low density lipoprotein (ox-LDL). A RT-qPCR to confirming TRAF5 expression in VSMCs treated with TRAF5 siRNAs. B - E Western blotting detecting the levels of RIPK3, MLKL, and p-MLKL in ox-LDL-induced VSMCs with TRAF5 knockdown ( n = 3, normalized to GAPDH). F Cell counting kit 8 assay (CCK8) evaluating cell proliferation ( n = 3). G , H EdU assay evaluating cell proliferation ( n = 3). I , J Necroptosis was detected by flow cytometry after Annexin V/PI staining. Annexin V-/PI- represents living cells, Annexin V + /PI- represents early necroptotic cells, and Annexin V + /PI + represents necroptotic cells ( n = 3). (ns: no significance; * p < 0.05; ** p < 0.01; *** p < 0.001)

    Article Snippet: The membrane was then incubated with primary antibodies against RIPK3 (1:3000; 17,563–1-AP; Proteintech Group, Inc., Wuhan, China), MLKL (1:800; 21,066–1-AP; Proteintech Group), and phospho-MLKL (S358) (1:800; T57146; Abmart Pharmaceutical Co., Ltd, Shanghai, China) at 4 °C overnight.

    Techniques: Quantitative RT-PCR, Expressing, Western Blot, Cell Counting, EdU Assay, Flow Cytometry, Staining

    Adjuvant chemotherapy regimen in  RIPK3  protein high- and low- expression groups

    Journal: Annals of Translational Medicine

    Article Title: Receptor-interacting protein kinase 3 as a predictive adjuvant chemotherapy marker after lung adenocarcinoma resection

    doi: 10.21037/atm.2018.12.65

    Figure Lengend Snippet: Adjuvant chemotherapy regimen in RIPK3 protein high- and low- expression groups

    Article Snippet: The membranes were blocked with 5% skimmed milk in tris-buffered saline containing Tween-20 and were incubated with primary antibodies against RIPK3 (Cell Signaling Technology, Danvers, MA) and β-actin (Santa Cruz Biotechnology, Dallas, TX).

    Techniques: Expressing

    RIPK3 expression in human LUAD tissues and adjacent normal epithelial tissues. (A) Western blotting analysis of RIPK3 expression levels. Proteins obtained from 11 adenocarcinoma tissue (T) specimens and pair-matched normal tissue (N) specimens were examined by performing Western blotting analysis. β-Actin was used as an internal control. The experiment was repeated three times independently and provided similar results. Values are shown as mean ± SD (n=11 replicates). RIPK3 expression levels in the N and T specimens are shown in a box-whisker plot. Significance was calculated using Mann-Whitney U test (P=0.877). (B): (a) representative image showing high RIPK3 expression, which was observed in the cytoplasm and nucleus of cancer cells; (b) RIPK3 staining was absent or minimal in the tissues showing low RIPK3 expression. Magnification: ×100 in the left panels and ×200 in the right panels. LUAD, lung adenocarcinoma.

    Journal: Annals of Translational Medicine

    Article Title: Receptor-interacting protein kinase 3 as a predictive adjuvant chemotherapy marker after lung adenocarcinoma resection

    doi: 10.21037/atm.2018.12.65

    Figure Lengend Snippet: RIPK3 expression in human LUAD tissues and adjacent normal epithelial tissues. (A) Western blotting analysis of RIPK3 expression levels. Proteins obtained from 11 adenocarcinoma tissue (T) specimens and pair-matched normal tissue (N) specimens were examined by performing Western blotting analysis. β-Actin was used as an internal control. The experiment was repeated three times independently and provided similar results. Values are shown as mean ± SD (n=11 replicates). RIPK3 expression levels in the N and T specimens are shown in a box-whisker plot. Significance was calculated using Mann-Whitney U test (P=0.877). (B): (a) representative image showing high RIPK3 expression, which was observed in the cytoplasm and nucleus of cancer cells; (b) RIPK3 staining was absent or minimal in the tissues showing low RIPK3 expression. Magnification: ×100 in the left panels and ×200 in the right panels. LUAD, lung adenocarcinoma.

    Article Snippet: The membranes were blocked with 5% skimmed milk in tris-buffered saline containing Tween-20 and were incubated with primary antibodies against RIPK3 (Cell Signaling Technology, Danvers, MA) and β-actin (Santa Cruz Biotechnology, Dallas, TX).

    Techniques: Expressing, Western Blot, Whisker Assay, MANN-WHITNEY, Staining

    RIP3 immunohistochemical data for lung adeno-NSCLC: association of  RIPK3  score with clinical characteristics

    Journal: Annals of Translational Medicine

    Article Title: Receptor-interacting protein kinase 3 as a predictive adjuvant chemotherapy marker after lung adenocarcinoma resection

    doi: 10.21037/atm.2018.12.65

    Figure Lengend Snippet: RIP3 immunohistochemical data for lung adeno-NSCLC: association of RIPK3 score with clinical characteristics

    Article Snippet: The membranes were blocked with 5% skimmed milk in tris-buffered saline containing Tween-20 and were incubated with primary antibodies against RIPK3 (Cell Signaling Technology, Danvers, MA) and β-actin (Santa Cruz Biotechnology, Dallas, TX).

    Techniques: Immunohistochemical staining

    Clinical characteristics of patients for  RIPK3  protein expression

    Journal: Annals of Translational Medicine

    Article Title: Receptor-interacting protein kinase 3 as a predictive adjuvant chemotherapy marker after lung adenocarcinoma resection

    doi: 10.21037/atm.2018.12.65

    Figure Lengend Snippet: Clinical characteristics of patients for RIPK3 protein expression

    Article Snippet: The membranes were blocked with 5% skimmed milk in tris-buffered saline containing Tween-20 and were incubated with primary antibodies against RIPK3 (Cell Signaling Technology, Danvers, MA) and β-actin (Santa Cruz Biotechnology, Dallas, TX).

    Techniques:

    The Kaplan-Meier curves obtained using the TMA specimens. The OS (A) and DFS (B) curves of the patients showing high and low RIPK3 expression levels who underwent the cisplatin doublet chemotherapy; (C) the OS curves obtained using the TCGA mRNA sequencing data. OS, overall survival; DFS, disease-free survival.

    Journal: Annals of Translational Medicine

    Article Title: Receptor-interacting protein kinase 3 as a predictive adjuvant chemotherapy marker after lung adenocarcinoma resection

    doi: 10.21037/atm.2018.12.65

    Figure Lengend Snippet: The Kaplan-Meier curves obtained using the TMA specimens. The OS (A) and DFS (B) curves of the patients showing high and low RIPK3 expression levels who underwent the cisplatin doublet chemotherapy; (C) the OS curves obtained using the TCGA mRNA sequencing data. OS, overall survival; DFS, disease-free survival.

    Article Snippet: The membranes were blocked with 5% skimmed milk in tris-buffered saline containing Tween-20 and were incubated with primary antibodies against RIPK3 (Cell Signaling Technology, Danvers, MA) and β-actin (Santa Cruz Biotechnology, Dallas, TX).

    Techniques: Expressing, Sequencing

    The relationship between  RIPK3  protein expression and treatment responses

    Journal: Annals of Translational Medicine

    Article Title: Receptor-interacting protein kinase 3 as a predictive adjuvant chemotherapy marker after lung adenocarcinoma resection

    doi: 10.21037/atm.2018.12.65

    Figure Lengend Snippet: The relationship between RIPK3 protein expression and treatment responses

    Article Snippet: The membranes were blocked with 5% skimmed milk in tris-buffered saline containing Tween-20 and were incubated with primary antibodies against RIPK3 (Cell Signaling Technology, Danvers, MA) and β-actin (Santa Cruz Biotechnology, Dallas, TX).

    Techniques: Expressing

    Univariate analysis of clinical variables and  RIPK3  in relation to the OS and DFS

    Journal: Annals of Translational Medicine

    Article Title: Receptor-interacting protein kinase 3 as a predictive adjuvant chemotherapy marker after lung adenocarcinoma resection

    doi: 10.21037/atm.2018.12.65

    Figure Lengend Snippet: Univariate analysis of clinical variables and RIPK3 in relation to the OS and DFS

    Article Snippet: The membranes were blocked with 5% skimmed milk in tris-buffered saline containing Tween-20 and were incubated with primary antibodies against RIPK3 (Cell Signaling Technology, Danvers, MA) and β-actin (Santa Cruz Biotechnology, Dallas, TX).

    Techniques:

    Immunofluorescence for RIPK3. (A) Immunofluorescence staining of RIPK3 in the cerebral cortex at 24 h after CA/CPR. Scale bar: 20 μm. The light color granules detected were positive cells. Scale bar (White short bar): 20 μm. (B) RIPK3-positive rate (RIPK3-positive area/DAPI-positive area) per group. All data are mean ± SEM. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. sham; ## p < 0.01 vs. CA; and & p < 0.05 vs. Gly. RIPK3: receptor-interacting serine/threonine kinase 3; CA: cardiac arrest/0.9% saline group; CPR: cardiopulmonary resuscitation; DAPI: 4′,6-diamidino-2-phenylindole; SEM: standard error of the mean; sham: sham-operated group; Gly: 10% glycerol group.

    Journal: Pharmaceutical Biology

    Article Title: Pomelo peel oil suppresses TNF-α-induced necroptosis and cerebral ischaemia–reperfusion injury in a rat model of cardiac arrest

    doi: 10.1080/13880209.2021.1903046

    Figure Lengend Snippet: Immunofluorescence for RIPK3. (A) Immunofluorescence staining of RIPK3 in the cerebral cortex at 24 h after CA/CPR. Scale bar: 20 μm. The light color granules detected were positive cells. Scale bar (White short bar): 20 μm. (B) RIPK3-positive rate (RIPK3-positive area/DAPI-positive area) per group. All data are mean ± SEM. * p < 0.05, ** p < 0.01 and *** p < 0.001 vs. sham; ## p < 0.01 vs. CA; and & p < 0.05 vs. Gly. RIPK3: receptor-interacting serine/threonine kinase 3; CA: cardiac arrest/0.9% saline group; CPR: cardiopulmonary resuscitation; DAPI: 4′,6-diamidino-2-phenylindole; SEM: standard error of the mean; sham: sham-operated group; Gly: 10% glycerol group.

    Article Snippet: After the slides were dewaxed, repaired and sealed, the sections were incubated overnight at 4 °C with monoclonal antibodies against RIPK3 (1:3000; Abcam, Cambridge, UK).

    Techniques: Immunofluorescence, Staining

    STING -/- mice display increased necroptosis during early S.aureus induced pneumonia. WT and STING -/- mice were infected intranasally with S.aureus (1 × 10 8 CFU/mouse) and then were euthanized at 6 hpi. (A, B) TUNEL staining of dead cells in the lung tissues. (C) LDH released in BALF. (D, E) The numbers of neutrophils and macrophages in the BALF were enumerated. (F, G) Propidium iodide positive (PI + ) macrophages in the BALF were analyzed by flow cytometry. (H) The lung tissue lysate was analyzed for p-MLKL, MLKL, and RIPK3 by western blotting. GAPDH was used as a loading control. Student’s t-test was performed. Statistical significance is indicated by *p < 0.05 and **p < 0.01.

    Journal: Frontiers in Immunology

    Article Title: STING Contributes to Host Defense Against Staphylococcus aureus Pneumonia Through Suppressing Necroptosis

    doi: 10.3389/fimmu.2021.636861

    Figure Lengend Snippet: STING -/- mice display increased necroptosis during early S.aureus induced pneumonia. WT and STING -/- mice were infected intranasally with S.aureus (1 × 10 8 CFU/mouse) and then were euthanized at 6 hpi. (A, B) TUNEL staining of dead cells in the lung tissues. (C) LDH released in BALF. (D, E) The numbers of neutrophils and macrophages in the BALF were enumerated. (F, G) Propidium iodide positive (PI + ) macrophages in the BALF were analyzed by flow cytometry. (H) The lung tissue lysate was analyzed for p-MLKL, MLKL, and RIPK3 by western blotting. GAPDH was used as a loading control. Student’s t-test was performed. Statistical significance is indicated by *p < 0.05 and **p < 0.01.

    Article Snippet: After blocking with 5% milk, the membranes were incubated with primary antibodies against RIPK3 (AP7819b, ABGENT, San Diego, CA, USA), p-MLKL (ab196436, Abcam, Cambridge, MA, USA), MLKL (MABC604, Millipore, Billerica, MA, USA), and GAPDH (10494-1-AP, Proteintech, Wuhan, Hubei, China).

    Techniques: Infection, TUNEL Assay, Staining, Flow Cytometry, Western Blot

    STING reduces activation of necroptosis in macrophages following S.aureus challenge. (A, C) WT and STING -/- BMDMs were untreated or exposed to S.aureus at MOI=50 for the indicated time. BMDMs from WT and STING -/- mice were pre-treated Nec-1 (50 μM) or an equivalent amount of DMSO for 1 h prior to infection with S.aureus . The cellular lysate was analyzed for RIPK3, p-MLKL, MLKL, and GAPDH by western blotting. (B) Cell death was measured by PI staining in BMDMs infected with S.aureus at MOI=50 for 4 h. (D) BMDMs from WT and STING -/- mice were infected with S. aureus (MOI=50) for 4 hours, p-MLKL activation was observed through fluorescence microscopy. All figures are representative of three independent experiments. Student’s t-test was performed. Statistical significance is indicated by *p < 0.05.

    Journal: Frontiers in Immunology

    Article Title: STING Contributes to Host Defense Against Staphylococcus aureus Pneumonia Through Suppressing Necroptosis

    doi: 10.3389/fimmu.2021.636861

    Figure Lengend Snippet: STING reduces activation of necroptosis in macrophages following S.aureus challenge. (A, C) WT and STING -/- BMDMs were untreated or exposed to S.aureus at MOI=50 for the indicated time. BMDMs from WT and STING -/- mice were pre-treated Nec-1 (50 μM) or an equivalent amount of DMSO for 1 h prior to infection with S.aureus . The cellular lysate was analyzed for RIPK3, p-MLKL, MLKL, and GAPDH by western blotting. (B) Cell death was measured by PI staining in BMDMs infected with S.aureus at MOI=50 for 4 h. (D) BMDMs from WT and STING -/- mice were infected with S. aureus (MOI=50) for 4 hours, p-MLKL activation was observed through fluorescence microscopy. All figures are representative of three independent experiments. Student’s t-test was performed. Statistical significance is indicated by *p < 0.05.

    Article Snippet: After blocking with 5% milk, the membranes were incubated with primary antibodies against RIPK3 (AP7819b, ABGENT, San Diego, CA, USA), p-MLKL (ab196436, Abcam, Cambridge, MA, USA), MLKL (MABC604, Millipore, Billerica, MA, USA), and GAPDH (10494-1-AP, Proteintech, Wuhan, Hubei, China).

    Techniques: Activation Assay, Infection, Western Blot, Staining, Fluorescence, Microscopy