antibody against aqp3 Search Results


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  • 88
    Millipore antibodies against aqp3
    CXCL12-induced directional migration requires <t>AQP3</t> and H 2 O 2 uptake. (A to F) MDA-MB-231 and DU4475 cells were transfected with control or AQP3 siRNA. The error bars indicate SE. (A) The chemotaxis efficiency of control or AQP3 KD MDA-MB-231 and DU4475
    Antibodies Against Aqp3, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against aqp3/product/Millipore
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against aqp3 - by Bioz Stars, 2022-08
    88/100 stars
      Buy from Supplier

    97
    Abcam antibodies against aqp3
    CXCL12-induced directional migration requires <t>AQP3</t> and H 2 O 2 uptake. (A to F) MDA-MB-231 and DU4475 cells were transfected with control or AQP3 siRNA. The error bars indicate SE. (A) The chemotaxis efficiency of control or AQP3 KD MDA-MB-231 and DU4475
    Antibodies Against Aqp3, supplied by Abcam, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against aqp3/product/Abcam
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against aqp3 - by Bioz Stars, 2022-08
    97/100 stars
      Buy from Supplier

    95
    Santa Cruz Biotechnology antibodies against aqp3
    Aquaporin 3 <t>(AQP3)</t> enhances autophagy in gastric cancer cells. ( a ) AQP3 overexpression induced the conversion of LC3-I to LC3-II in AGS cells, and AQP3 knockdown inhibited this conversion in MGC803 and SGC7901 cells ( b , * P
    Antibodies Against Aqp3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against aqp3/product/Santa Cruz Biotechnology
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against aqp3 - by Bioz Stars, 2022-08
    95/100 stars
      Buy from Supplier

    93
    Alomone Labs antibody against aqp3
    A549 cell aggregation is an active process. ( A ) The effect of ROCK on A549 cell aggregation. The phase-contrast micrographs show the morphologies of the A549 cells following treatment with a ROCK inhibitor, Y-27632, and the Rho activator Ⅱ. ( B ) The effects of <t>AQP3</t> knockdown with siRNA on actomyosin cytoskeleton remodeling. A549 cells were stained with anti-AQP3 antibody, followed by Fluorescein-conjugated antibody (green). The actin microfilaments were stained with rhodamine-conjugated phalloidin (red), and the nuclei were stained with DAPI (blue). ( C ) The effects of AQP3 knockdown with siRNA on the apoptosis signaling pathway. Twenty-four h following siRNA transfection in 2D culture condition, the cells were further incubated in the 2D or 3D culture condition. Then, cells were harvested to confirm the effect of AQP3 knockdown on the apoptotic molecular signatures using Western blotting. α-tubulin was used as an internal control.
    Antibody Against Aqp3, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibody against aqp3/product/Alomone Labs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibody against aqp3 - by Bioz Stars, 2022-08
    93/100 stars
      Buy from Supplier

    Image Search Results


    CXCL12-induced directional migration requires AQP3 and H 2 O 2 uptake. (A to F) MDA-MB-231 and DU4475 cells were transfected with control or AQP3 siRNA. The error bars indicate SE. (A) The chemotaxis efficiency of control or AQP3 KD MDA-MB-231 and DU4475

    Journal: Molecular and Cellular Biology

    Article Title: Aquaporin-3 Controls Breast Cancer Cell Migration by Regulating Hydrogen Peroxide Transport and Its Downstream Cell Signaling

    doi: 10.1128/MCB.00971-15

    Figure Lengend Snippet: CXCL12-induced directional migration requires AQP3 and H 2 O 2 uptake. (A to F) MDA-MB-231 and DU4475 cells were transfected with control or AQP3 siRNA. The error bars indicate SE. (A) The chemotaxis efficiency of control or AQP3 KD MDA-MB-231 and DU4475

    Article Snippet: For AQP3 staining and visualization of macrophage infiltration, frozen or paraffin-embedded sections were stained with antibodies against AQP3 (Millipore), human EpCAM (eFluor 660; eBioscience), turbo-GFP (Origene), or F4/80 (allophycocyanin [APC]; Biolegend).

    Techniques: Migration, Multiple Displacement Amplification, Transfection, Chemotaxis Assay

    AQP3-dependent PTEN/PTP1B oxidation and Akt phosphorylation. (A to E) MDA-MB-231 cells were infected with lentiviral control or AQP3 shRNA, and DU4475 cells were transfected with control or AQP3 siRNA. (A) Immunoblot analysis of PTEN oxidation. Control

    Journal: Molecular and Cellular Biology

    Article Title: Aquaporin-3 Controls Breast Cancer Cell Migration by Regulating Hydrogen Peroxide Transport and Its Downstream Cell Signaling

    doi: 10.1128/MCB.00971-15

    Figure Lengend Snippet: AQP3-dependent PTEN/PTP1B oxidation and Akt phosphorylation. (A to E) MDA-MB-231 cells were infected with lentiviral control or AQP3 shRNA, and DU4475 cells were transfected with control or AQP3 siRNA. (A) Immunoblot analysis of PTEN oxidation. Control

    Article Snippet: For AQP3 staining and visualization of macrophage infiltration, frozen or paraffin-embedded sections were stained with antibodies against AQP3 (Millipore), human EpCAM (eFluor 660; eBioscience), turbo-GFP (Origene), or F4/80 (allophycocyanin [APC]; Biolegend).

    Techniques: Multiple Displacement Amplification, Infection, shRNA, Transfection

    Involvement of AQP3 expression in breast cancer cell migration in vivo . The error bars indicate SE. (A to D) MDA-MB-231 cells (8.0 × 10 5 cells) were intravenously injected into SCID mice, and the numbers of cells in the lungs after 24 h were analyzed

    Journal: Molecular and Cellular Biology

    Article Title: Aquaporin-3 Controls Breast Cancer Cell Migration by Regulating Hydrogen Peroxide Transport and Its Downstream Cell Signaling

    doi: 10.1128/MCB.00971-15

    Figure Lengend Snippet: Involvement of AQP3 expression in breast cancer cell migration in vivo . The error bars indicate SE. (A to D) MDA-MB-231 cells (8.0 × 10 5 cells) were intravenously injected into SCID mice, and the numbers of cells in the lungs after 24 h were analyzed

    Article Snippet: For AQP3 staining and visualization of macrophage infiltration, frozen or paraffin-embedded sections were stained with antibodies against AQP3 (Millipore), human EpCAM (eFluor 660; eBioscience), turbo-GFP (Origene), or F4/80 (allophycocyanin [APC]; Biolegend).

    Techniques: Expressing, Migration, In Vivo, Multiple Displacement Amplification, Injection, Mouse Assay

    CXCL12-induced H 2 O 2 regulates PTP1B/PTEN oxidation and Akt activation. (A to C, E, and F) MDA-MB-231 cells were infected with lentiviral control or AQP3 shRNA. (A and B) The effects of DPI (20 μM; 30 min) or catalase (2,000 U/ml) on PTEN (A) or

    Journal: Molecular and Cellular Biology

    Article Title: Aquaporin-3 Controls Breast Cancer Cell Migration by Regulating Hydrogen Peroxide Transport and Its Downstream Cell Signaling

    doi: 10.1128/MCB.00971-15

    Figure Lengend Snippet: CXCL12-induced H 2 O 2 regulates PTP1B/PTEN oxidation and Akt activation. (A to C, E, and F) MDA-MB-231 cells were infected with lentiviral control or AQP3 shRNA. (A and B) The effects of DPI (20 μM; 30 min) or catalase (2,000 U/ml) on PTEN (A) or

    Article Snippet: For AQP3 staining and visualization of macrophage infiltration, frozen or paraffin-embedded sections were stained with antibodies against AQP3 (Millipore), human EpCAM (eFluor 660; eBioscience), turbo-GFP (Origene), or F4/80 (allophycocyanin [APC]; Biolegend).

    Techniques: Activation Assay, Multiple Displacement Amplification, Infection, shRNA

    Transport of CXCL12-induced H 2 O 2 into breast cancer cells through AQP3. (A) The mRNA levels of AQP3 in control (si-control)- or AQP3-siRNA (si-AQP3)-transfected MDA-MB-231 (top) and DU4475 (bottom) cells and in lentiviral control (sh-control)- or AQP3-shRNA

    Journal: Molecular and Cellular Biology

    Article Title: Aquaporin-3 Controls Breast Cancer Cell Migration by Regulating Hydrogen Peroxide Transport and Its Downstream Cell Signaling

    doi: 10.1128/MCB.00971-15

    Figure Lengend Snippet: Transport of CXCL12-induced H 2 O 2 into breast cancer cells through AQP3. (A) The mRNA levels of AQP3 in control (si-control)- or AQP3-siRNA (si-AQP3)-transfected MDA-MB-231 (top) and DU4475 (bottom) cells and in lentiviral control (sh-control)- or AQP3-shRNA

    Article Snippet: For AQP3 staining and visualization of macrophage infiltration, frozen or paraffin-embedded sections were stained with antibodies against AQP3 (Millipore), human EpCAM (eFluor 660; eBioscience), turbo-GFP (Origene), or F4/80 (allophycocyanin [APC]; Biolegend).

    Techniques: Transfection, Multiple Displacement Amplification, shRNA

    AQP3 knockdown abrogates spontaneous metastasis to the lungs. (A to F) Control or AQP3 KD MDA-MB-231 cells (turbo-GFP-expressing control or AQP3 shRNA; 1.0 × 10 6 cells) were orthotopically injected into SCID mice. Primary tumors and lungs were

    Journal: Molecular and Cellular Biology

    Article Title: Aquaporin-3 Controls Breast Cancer Cell Migration by Regulating Hydrogen Peroxide Transport and Its Downstream Cell Signaling

    doi: 10.1128/MCB.00971-15

    Figure Lengend Snippet: AQP3 knockdown abrogates spontaneous metastasis to the lungs. (A to F) Control or AQP3 KD MDA-MB-231 cells (turbo-GFP-expressing control or AQP3 shRNA; 1.0 × 10 6 cells) were orthotopically injected into SCID mice. Primary tumors and lungs were

    Article Snippet: For AQP3 staining and visualization of macrophage infiltration, frozen or paraffin-embedded sections were stained with antibodies against AQP3 (Millipore), human EpCAM (eFluor 660; eBioscience), turbo-GFP (Origene), or F4/80 (allophycocyanin [APC]; Biolegend).

    Techniques: Multiple Displacement Amplification, Expressing, shRNA, Injection, Mouse Assay

    AQP3 overexpression increases H 2 O 2 uptake and cell migration upon CXCL12 stimulation. The error bars indicate SE. (A to F) MDA-MB-231 cells were transfected with the vector pCMV6 (empty [Emp]) or human AQP3-expressing pCMV6 (AQP3; 1 to 10 ng for 1.5 ×

    Journal: Molecular and Cellular Biology

    Article Title: Aquaporin-3 Controls Breast Cancer Cell Migration by Regulating Hydrogen Peroxide Transport and Its Downstream Cell Signaling

    doi: 10.1128/MCB.00971-15

    Figure Lengend Snippet: AQP3 overexpression increases H 2 O 2 uptake and cell migration upon CXCL12 stimulation. The error bars indicate SE. (A to F) MDA-MB-231 cells were transfected with the vector pCMV6 (empty [Emp]) or human AQP3-expressing pCMV6 (AQP3; 1 to 10 ng for 1.5 ×

    Article Snippet: For AQP3 staining and visualization of macrophage infiltration, frozen or paraffin-embedded sections were stained with antibodies against AQP3 (Millipore), human EpCAM (eFluor 660; eBioscience), turbo-GFP (Origene), or F4/80 (allophycocyanin [APC]; Biolegend).

    Techniques: Over Expression, Migration, Multiple Displacement Amplification, Transfection, Plasmid Preparation, Expressing

    CXCL12-induced directional migration requires AQP3 and H 2 O 2 uptake. (A to F) MDA-MB-231 and DU4475 cells were transfected with control or AQP3 siRNA. The error bars indicate SE. (A) The chemotaxis efficiency of control or AQP3 KD MDA-MB-231 and DU4475

    Journal: Molecular and Cellular Biology

    Article Title: Aquaporin-3 Controls Breast Cancer Cell Migration by Regulating Hydrogen Peroxide Transport and Its Downstream Cell Signaling

    doi: 10.1128/MCB.00971-15

    Figure Lengend Snippet: CXCL12-induced directional migration requires AQP3 and H 2 O 2 uptake. (A to F) MDA-MB-231 and DU4475 cells were transfected with control or AQP3 siRNA. The error bars indicate SE. (A) The chemotaxis efficiency of control or AQP3 KD MDA-MB-231 and DU4475

    Article Snippet: The supernatant (10,000 rpm; 10 min; 4°C) was used for immunoblotting with antibodies against AQP3 (Abcam), Na+ /K+-ATPase (Millipore), CD98 (Abcam), or Nox2 (Millipore).

    Techniques: Migration, Multiple Displacement Amplification, Transfection, Chemotaxis Assay

    AQP3-dependent PTEN/PTP1B oxidation and Akt phosphorylation. (A to E) MDA-MB-231 cells were infected with lentiviral control or AQP3 shRNA, and DU4475 cells were transfected with control or AQP3 siRNA. (A) Immunoblot analysis of PTEN oxidation. Control

    Journal: Molecular and Cellular Biology

    Article Title: Aquaporin-3 Controls Breast Cancer Cell Migration by Regulating Hydrogen Peroxide Transport and Its Downstream Cell Signaling

    doi: 10.1128/MCB.00971-15

    Figure Lengend Snippet: AQP3-dependent PTEN/PTP1B oxidation and Akt phosphorylation. (A to E) MDA-MB-231 cells were infected with lentiviral control or AQP3 shRNA, and DU4475 cells were transfected with control or AQP3 siRNA. (A) Immunoblot analysis of PTEN oxidation. Control

    Article Snippet: The supernatant (10,000 rpm; 10 min; 4°C) was used for immunoblotting with antibodies against AQP3 (Abcam), Na+ /K+-ATPase (Millipore), CD98 (Abcam), or Nox2 (Millipore).

    Techniques: Multiple Displacement Amplification, Infection, shRNA, Transfection

    Involvement of AQP3 expression in breast cancer cell migration in vivo . The error bars indicate SE. (A to D) MDA-MB-231 cells (8.0 × 10 5 cells) were intravenously injected into SCID mice, and the numbers of cells in the lungs after 24 h were analyzed

    Journal: Molecular and Cellular Biology

    Article Title: Aquaporin-3 Controls Breast Cancer Cell Migration by Regulating Hydrogen Peroxide Transport and Its Downstream Cell Signaling

    doi: 10.1128/MCB.00971-15

    Figure Lengend Snippet: Involvement of AQP3 expression in breast cancer cell migration in vivo . The error bars indicate SE. (A to D) MDA-MB-231 cells (8.0 × 10 5 cells) were intravenously injected into SCID mice, and the numbers of cells in the lungs after 24 h were analyzed

    Article Snippet: The supernatant (10,000 rpm; 10 min; 4°C) was used for immunoblotting with antibodies against AQP3 (Abcam), Na+ /K+-ATPase (Millipore), CD98 (Abcam), or Nox2 (Millipore).

    Techniques: Expressing, Migration, In Vivo, Multiple Displacement Amplification, Injection, Mouse Assay

    CXCL12-induced H 2 O 2 regulates PTP1B/PTEN oxidation and Akt activation. (A to C, E, and F) MDA-MB-231 cells were infected with lentiviral control or AQP3 shRNA. (A and B) The effects of DPI (20 μM; 30 min) or catalase (2,000 U/ml) on PTEN (A) or

    Journal: Molecular and Cellular Biology

    Article Title: Aquaporin-3 Controls Breast Cancer Cell Migration by Regulating Hydrogen Peroxide Transport and Its Downstream Cell Signaling

    doi: 10.1128/MCB.00971-15

    Figure Lengend Snippet: CXCL12-induced H 2 O 2 regulates PTP1B/PTEN oxidation and Akt activation. (A to C, E, and F) MDA-MB-231 cells were infected with lentiviral control or AQP3 shRNA. (A and B) The effects of DPI (20 μM; 30 min) or catalase (2,000 U/ml) on PTEN (A) or

    Article Snippet: The supernatant (10,000 rpm; 10 min; 4°C) was used for immunoblotting with antibodies against AQP3 (Abcam), Na+ /K+-ATPase (Millipore), CD98 (Abcam), or Nox2 (Millipore).

    Techniques: Activation Assay, Multiple Displacement Amplification, Infection, shRNA

    Transport of CXCL12-induced H 2 O 2 into breast cancer cells through AQP3. (A) The mRNA levels of AQP3 in control (si-control)- or AQP3-siRNA (si-AQP3)-transfected MDA-MB-231 (top) and DU4475 (bottom) cells and in lentiviral control (sh-control)- or AQP3-shRNA

    Journal: Molecular and Cellular Biology

    Article Title: Aquaporin-3 Controls Breast Cancer Cell Migration by Regulating Hydrogen Peroxide Transport and Its Downstream Cell Signaling

    doi: 10.1128/MCB.00971-15

    Figure Lengend Snippet: Transport of CXCL12-induced H 2 O 2 into breast cancer cells through AQP3. (A) The mRNA levels of AQP3 in control (si-control)- or AQP3-siRNA (si-AQP3)-transfected MDA-MB-231 (top) and DU4475 (bottom) cells and in lentiviral control (sh-control)- or AQP3-shRNA

    Article Snippet: The supernatant (10,000 rpm; 10 min; 4°C) was used for immunoblotting with antibodies against AQP3 (Abcam), Na+ /K+-ATPase (Millipore), CD98 (Abcam), or Nox2 (Millipore).

    Techniques: Transfection, Multiple Displacement Amplification, shRNA

    AQP3 knockdown abrogates spontaneous metastasis to the lungs. (A to F) Control or AQP3 KD MDA-MB-231 cells (turbo-GFP-expressing control or AQP3 shRNA; 1.0 × 10 6 cells) were orthotopically injected into SCID mice. Primary tumors and lungs were

    Journal: Molecular and Cellular Biology

    Article Title: Aquaporin-3 Controls Breast Cancer Cell Migration by Regulating Hydrogen Peroxide Transport and Its Downstream Cell Signaling

    doi: 10.1128/MCB.00971-15

    Figure Lengend Snippet: AQP3 knockdown abrogates spontaneous metastasis to the lungs. (A to F) Control or AQP3 KD MDA-MB-231 cells (turbo-GFP-expressing control or AQP3 shRNA; 1.0 × 10 6 cells) were orthotopically injected into SCID mice. Primary tumors and lungs were

    Article Snippet: The supernatant (10,000 rpm; 10 min; 4°C) was used for immunoblotting with antibodies against AQP3 (Abcam), Na+ /K+-ATPase (Millipore), CD98 (Abcam), or Nox2 (Millipore).

    Techniques: Multiple Displacement Amplification, Expressing, shRNA, Injection, Mouse Assay

    AQP3 overexpression increases H 2 O 2 uptake and cell migration upon CXCL12 stimulation. The error bars indicate SE. (A to F) MDA-MB-231 cells were transfected with the vector pCMV6 (empty [Emp]) or human AQP3-expressing pCMV6 (AQP3; 1 to 10 ng for 1.5 ×

    Journal: Molecular and Cellular Biology

    Article Title: Aquaporin-3 Controls Breast Cancer Cell Migration by Regulating Hydrogen Peroxide Transport and Its Downstream Cell Signaling

    doi: 10.1128/MCB.00971-15

    Figure Lengend Snippet: AQP3 overexpression increases H 2 O 2 uptake and cell migration upon CXCL12 stimulation. The error bars indicate SE. (A to F) MDA-MB-231 cells were transfected with the vector pCMV6 (empty [Emp]) or human AQP3-expressing pCMV6 (AQP3; 1 to 10 ng for 1.5 ×

    Article Snippet: The supernatant (10,000 rpm; 10 min; 4°C) was used for immunoblotting with antibodies against AQP3 (Abcam), Na+ /K+-ATPase (Millipore), CD98 (Abcam), or Nox2 (Millipore).

    Techniques: Over Expression, Migration, Multiple Displacement Amplification, Transfection, Plasmid Preparation, Expressing

    Aquaporin 3 (AQP3) enhances autophagy in gastric cancer cells. ( a ) AQP3 overexpression induced the conversion of LC3-I to LC3-II in AGS cells, and AQP3 knockdown inhibited this conversion in MGC803 and SGC7901 cells ( b , * P

    Journal: Cell Death Discovery

    Article Title: Aquaporin 3 facilitates chemoresistance in gastric cancer cells to cisplatin via autophagy

    doi: 10.1038/cddiscovery.2016.87

    Figure Lengend Snippet: Aquaporin 3 (AQP3) enhances autophagy in gastric cancer cells. ( a ) AQP3 overexpression induced the conversion of LC3-I to LC3-II in AGS cells, and AQP3 knockdown inhibited this conversion in MGC803 and SGC7901 cells ( b , * P

    Article Snippet: Western blotting Western blotting was performed to analyze the expression of proteins according to our published methods., We used antibodies against AQP3 (Santa Cruz Biotechnology, Santa Cruz, CA, USA), LC3 A/B (Cell Signaling Technology) and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (Beyotime Institute of Biotechnology).

    Techniques: Over Expression

    A549 cell aggregation is an active process. ( A ) The effect of ROCK on A549 cell aggregation. The phase-contrast micrographs show the morphologies of the A549 cells following treatment with a ROCK inhibitor, Y-27632, and the Rho activator Ⅱ. ( B ) The effects of AQP3 knockdown with siRNA on actomyosin cytoskeleton remodeling. A549 cells were stained with anti-AQP3 antibody, followed by Fluorescein-conjugated antibody (green). The actin microfilaments were stained with rhodamine-conjugated phalloidin (red), and the nuclei were stained with DAPI (blue). ( C ) The effects of AQP3 knockdown with siRNA on the apoptosis signaling pathway. Twenty-four h following siRNA transfection in 2D culture condition, the cells were further incubated in the 2D or 3D culture condition. Then, cells were harvested to confirm the effect of AQP3 knockdown on the apoptotic molecular signatures using Western blotting. α-tubulin was used as an internal control.

    Journal: International Journal of Molecular Sciences

    Article Title: AQP3 Increases Intercellular Cohesion in NSCLC A549 Cell Spheroids through Exploratory Cell Protrusions

    doi: 10.3390/ijms22084287

    Figure Lengend Snippet: A549 cell aggregation is an active process. ( A ) The effect of ROCK on A549 cell aggregation. The phase-contrast micrographs show the morphologies of the A549 cells following treatment with a ROCK inhibitor, Y-27632, and the Rho activator Ⅱ. ( B ) The effects of AQP3 knockdown with siRNA on actomyosin cytoskeleton remodeling. A549 cells were stained with anti-AQP3 antibody, followed by Fluorescein-conjugated antibody (green). The actin microfilaments were stained with rhodamine-conjugated phalloidin (red), and the nuclei were stained with DAPI (blue). ( C ) The effects of AQP3 knockdown with siRNA on the apoptosis signaling pathway. Twenty-four h following siRNA transfection in 2D culture condition, the cells were further incubated in the 2D or 3D culture condition. Then, cells were harvested to confirm the effect of AQP3 knockdown on the apoptotic molecular signatures using Western blotting. α-tubulin was used as an internal control.

    Article Snippet: In short, the cells on coverslips were fixed with 4% paraformaldehyde for 10 min and permeabilized with 0.15% Triton-X 100 for 5 min. Then, the cells were blocked for 1 h with the blocking solution of 3% bovine serum albumin in PBS and incubated with the primary antibody against AQP3 for 2 h at room temperature.

    Techniques: Staining, Transfection, Incubation, Western Blot

    The effect of AQP3 on spatiotemporal dynamics of protrusions. ( A ) Polymerase chain reaction and ( B ) Quantitative real-time reverse transcription-polymerase chain reaction of the transcript levels of the organic hydroxyl transport genes. The data shown here represent three independent experiments, and the values represent the mean ± SEM of triplicate samples. The level of each mRNA was normalized to that of the GAPDH mRNA in the same sample and presented as the fold-change over that of the 2D culture control cells. The differences in expression levels were evaluated for significance using two-tailed t-tests with unequal variance. * p

    Journal: International Journal of Molecular Sciences

    Article Title: AQP3 Increases Intercellular Cohesion in NSCLC A549 Cell Spheroids through Exploratory Cell Protrusions

    doi: 10.3390/ijms22084287

    Figure Lengend Snippet: The effect of AQP3 on spatiotemporal dynamics of protrusions. ( A ) Polymerase chain reaction and ( B ) Quantitative real-time reverse transcription-polymerase chain reaction of the transcript levels of the organic hydroxyl transport genes. The data shown here represent three independent experiments, and the values represent the mean ± SEM of triplicate samples. The level of each mRNA was normalized to that of the GAPDH mRNA in the same sample and presented as the fold-change over that of the 2D culture control cells. The differences in expression levels were evaluated for significance using two-tailed t-tests with unequal variance. * p

    Article Snippet: In short, the cells on coverslips were fixed with 4% paraformaldehyde for 10 min and permeabilized with 0.15% Triton-X 100 for 5 min. Then, the cells were blocked for 1 h with the blocking solution of 3% bovine serum albumin in PBS and incubated with the primary antibody against AQP3 for 2 h at room temperature.

    Techniques: Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Expressing, Two Tailed Test