antibodies against kir6 1 Search Results


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  • 91
    Alomone Labs antibodies against kir6 1
    The expression of K ATP subtypes in ECFC s. ( A ) RT ‐ PCR showed the expression of <t>Kir6.1,</t> Kir6.2 and SUR 2b, but not SUR 2a and SUR 1 in mRNA level. HPAEC s and mouse brain were used as positive controls, water (no template) as a negative control ( n = 3). ( B ) Western blotting confirmed the expression of Kir6.1 (48 kD), Kir6.2 (44 kD) and SUR 2b (140–150 kD), but not SUR 2a (140–150 kD) and SUR 1 (175 kD), using HPAEC s and mouse brain as positive controls ( n = 3). ( C ) Confocal images showed the subcellular localization of K ATP subunits in ECFC s co‐stained with a endothelial specific marker ( CD 31 or VE ‐cadherin), revealing the extensive distribution of Kir6.1, Kir6.2 and SUR 2b. DAPI staining for nuclear labelling ( n = 3), scale bar: 20 μm. M: marker, MB : mouse brain.
    Antibodies Against Kir6 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against kir6 1/product/Alomone Labs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against kir6 1 - by Bioz Stars, 2022-10
    91/100 stars
      Buy from Supplier

    80
    Santa Cruz Biotechnology antibodies against kir6 1
    The expression of K ATP subtypes in ECFC s. ( A ) RT ‐ PCR showed the expression of <t>Kir6.1,</t> Kir6.2 and SUR 2b, but not SUR 2a and SUR 1 in mRNA level. HPAEC s and mouse brain were used as positive controls, water (no template) as a negative control ( n = 3). ( B ) Western blotting confirmed the expression of Kir6.1 (48 kD), Kir6.2 (44 kD) and SUR 2b (140–150 kD), but not SUR 2a (140–150 kD) and SUR 1 (175 kD), using HPAEC s and mouse brain as positive controls ( n = 3). ( C ) Confocal images showed the subcellular localization of K ATP subunits in ECFC s co‐stained with a endothelial specific marker ( CD 31 or VE ‐cadherin), revealing the extensive distribution of Kir6.1, Kir6.2 and SUR 2b. DAPI staining for nuclear labelling ( n = 3), scale bar: 20 μm. M: marker, MB : mouse brain.
    Antibodies Against Kir6 1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against kir6 1/product/Santa Cruz Biotechnology
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against kir6 1 - by Bioz Stars, 2022-10
    80/100 stars
      Buy from Supplier

    93
    Millipore antibodies against kir6 1
    Effects of MGO and miR-9a-3p on functional ATP-sensitive K + (K ATP ) currents. One day after <t>Kir6.1/SUR2B</t> channels were expressed in HEK293 cells, the cells transfected with anti-9 were treated with 300 μM MGO and cultured for 12–24 h. Cells transfected with scmiR were used as negative control. A : K ATP currents were recorded using symmetric K + concentrations of internal and bath solutions. Inward K + currents were elicited with voltage commands from 0 to −80 mV every 3 s. K ATP currents were strongly activated by 10 μM pinacidil (Pin) and were inhibited by 10 μM glibenclamide (Glib). BL, baseline. B : MGO treatment significantly suppressed K ATP current in HEK cells. C : MGO-induced reduction of K ATP currents was reversed in anti-9-transfected cells. D : current density is represented in the bar graph. ** P
    Antibodies Against Kir6 1, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against kir6 1/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against kir6 1 - by Bioz Stars, 2022-10
    93/100 stars
      Buy from Supplier

    Image Search Results


    The expression of K ATP subtypes in ECFC s. ( A ) RT ‐ PCR showed the expression of Kir6.1, Kir6.2 and SUR 2b, but not SUR 2a and SUR 1 in mRNA level. HPAEC s and mouse brain were used as positive controls, water (no template) as a negative control ( n = 3). ( B ) Western blotting confirmed the expression of Kir6.1 (48 kD), Kir6.2 (44 kD) and SUR 2b (140–150 kD), but not SUR 2a (140–150 kD) and SUR 1 (175 kD), using HPAEC s and mouse brain as positive controls ( n = 3). ( C ) Confocal images showed the subcellular localization of K ATP subunits in ECFC s co‐stained with a endothelial specific marker ( CD 31 or VE ‐cadherin), revealing the extensive distribution of Kir6.1, Kir6.2 and SUR 2b. DAPI staining for nuclear labelling ( n = 3), scale bar: 20 μm. M: marker, MB : mouse brain.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Activation of ATP‐sensitive potassium channels facilitates the function of human endothelial colony‐forming cells via Ca2+/Akt/ eNOS pathway

    doi: 10.1111/jcmm.13006

    Figure Lengend Snippet: The expression of K ATP subtypes in ECFC s. ( A ) RT ‐ PCR showed the expression of Kir6.1, Kir6.2 and SUR 2b, but not SUR 2a and SUR 1 in mRNA level. HPAEC s and mouse brain were used as positive controls, water (no template) as a negative control ( n = 3). ( B ) Western blotting confirmed the expression of Kir6.1 (48 kD), Kir6.2 (44 kD) and SUR 2b (140–150 kD), but not SUR 2a (140–150 kD) and SUR 1 (175 kD), using HPAEC s and mouse brain as positive controls ( n = 3). ( C ) Confocal images showed the subcellular localization of K ATP subunits in ECFC s co‐stained with a endothelial specific marker ( CD 31 or VE ‐cadherin), revealing the extensive distribution of Kir6.1, Kir6.2 and SUR 2b. DAPI staining for nuclear labelling ( n = 3), scale bar: 20 μm. M: marker, MB : mouse brain.

    Article Snippet: Confocal microscopy In brief, after fixation and blocking, ECFCs were permeabilized with 0.5% Triton X‐100 (Sigma‐Aldrich) in PBS, blocked with 5% BSA for 1 hr at room temperature and incubated with antibodies against Kir6.1 (Alomone Labs), Kir6.2 (Abcam), SUR2b (Santa Cruz), CD31 (Santa Cruz) and VE‐cadherin (Abcam) at 4°C overnight.

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Negative Control, Western Blot, Staining, Marker

    Effects of MGO and miR-9a-3p on functional ATP-sensitive K + (K ATP ) currents. One day after Kir6.1/SUR2B channels were expressed in HEK293 cells, the cells transfected with anti-9 were treated with 300 μM MGO and cultured for 12–24 h. Cells transfected with scmiR were used as negative control. A : K ATP currents were recorded using symmetric K + concentrations of internal and bath solutions. Inward K + currents were elicited with voltage commands from 0 to −80 mV every 3 s. K ATP currents were strongly activated by 10 μM pinacidil (Pin) and were inhibited by 10 μM glibenclamide (Glib). BL, baseline. B : MGO treatment significantly suppressed K ATP current in HEK cells. C : MGO-induced reduction of K ATP currents was reversed in anti-9-transfected cells. D : current density is represented in the bar graph. ** P

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: The SUR2B subunit of rat vascular KATP channel is targeted by miR-9a-3p induced by prolonged exposure to methylglyoxal

    doi: 10.1152/ajpcell.00311.2014

    Figure Lengend Snippet: Effects of MGO and miR-9a-3p on functional ATP-sensitive K + (K ATP ) currents. One day after Kir6.1/SUR2B channels were expressed in HEK293 cells, the cells transfected with anti-9 were treated with 300 μM MGO and cultured for 12–24 h. Cells transfected with scmiR were used as negative control. A : K ATP currents were recorded using symmetric K + concentrations of internal and bath solutions. Inward K + currents were elicited with voltage commands from 0 to −80 mV every 3 s. K ATP currents were strongly activated by 10 μM pinacidil (Pin) and were inhibited by 10 μM glibenclamide (Glib). BL, baseline. B : MGO treatment significantly suppressed K ATP current in HEK cells. C : MGO-induced reduction of K ATP currents was reversed in anti-9-transfected cells. D : current density is represented in the bar graph. ** P

    Article Snippet: Antibodies against Kir6.1 and GAPDH were purchased from Sigma-Aldrich (St. Louis, MO).

    Techniques: Functional Assay, Transfection, Cell Culture, Negative Control

    Profiling of methylglyoxal (MGO)-regulated microRNAs (miRs) in A10 cells. A10 cells were treated with 300 μM MGO for 6 h. Real-time quantitative PCR (qPCR) analysis was then performed to show changes in expression levels of candidate miRs targeting Kir6.1 ( A ) and SUR2B mRNA ( B ). RNU6 (U6) was used for normalization. ** P

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: The SUR2B subunit of rat vascular KATP channel is targeted by miR-9a-3p induced by prolonged exposure to methylglyoxal

    doi: 10.1152/ajpcell.00311.2014

    Figure Lengend Snippet: Profiling of methylglyoxal (MGO)-regulated microRNAs (miRs) in A10 cells. A10 cells were treated with 300 μM MGO for 6 h. Real-time quantitative PCR (qPCR) analysis was then performed to show changes in expression levels of candidate miRs targeting Kir6.1 ( A ) and SUR2B mRNA ( B ). RNU6 (U6) was used for normalization. ** P

    Article Snippet: Antibodies against Kir6.1 and GAPDH were purchased from Sigma-Aldrich (St. Louis, MO).

    Techniques: Real-time Polymerase Chain Reaction, Expressing

    Mutation sites in Kir6.1 and SUR2B mRNA. A : evolutionary conservation of miR-9a-3p among mammals. B: sequences of SUR2B mRNA (SUR). C : sequences of mutated SUR2B mRNA (M-SUR). Mutated sites were numbered in SUR2B mRNA. Some omitted sequences are represented with “N”. Underlined letters are targeting sites for miR-9a-3p.

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: The SUR2B subunit of rat vascular KATP channel is targeted by miR-9a-3p induced by prolonged exposure to methylglyoxal

    doi: 10.1152/ajpcell.00311.2014

    Figure Lengend Snippet: Mutation sites in Kir6.1 and SUR2B mRNA. A : evolutionary conservation of miR-9a-3p among mammals. B: sequences of SUR2B mRNA (SUR). C : sequences of mutated SUR2B mRNA (M-SUR). Mutated sites were numbered in SUR2B mRNA. Some omitted sequences are represented with “N”. Underlined letters are targeting sites for miR-9a-3p.

    Article Snippet: Antibodies against Kir6.1 and GAPDH were purchased from Sigma-Aldrich (St. Louis, MO).

    Techniques: Mutagenesis

    Effects of miR-9a-3p on MGO-induced inhibition of SUR2B/Kir6.1 expression at the protein level. After transfection with m-9, A10 cells were cultured for 12–24 h. Cells transfected with anti-9 were also treated with 300 μM MGO and cultured under the same conditions. Cells transfected with scmiR were used as negative control, and GAPDH was used for normalization. A and C : Western blot assay of expression of protein levels of SUR2B ( A ) and Kir6.1 ( C ). B and D : bar graphs represent the photodensity of each protein. * P

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: The SUR2B subunit of rat vascular KATP channel is targeted by miR-9a-3p induced by prolonged exposure to methylglyoxal

    doi: 10.1152/ajpcell.00311.2014

    Figure Lengend Snippet: Effects of miR-9a-3p on MGO-induced inhibition of SUR2B/Kir6.1 expression at the protein level. After transfection with m-9, A10 cells were cultured for 12–24 h. Cells transfected with anti-9 were also treated with 300 μM MGO and cultured under the same conditions. Cells transfected with scmiR were used as negative control, and GAPDH was used for normalization. A and C : Western blot assay of expression of protein levels of SUR2B ( A ) and Kir6.1 ( C ). B and D : bar graphs represent the photodensity of each protein. * P

    Article Snippet: Antibodies against Kir6.1 and GAPDH were purchased from Sigma-Aldrich (St. Louis, MO).

    Techniques: Inhibition, Expressing, Transfection, Cell Culture, Negative Control, Western Blot