antibodies against il 27 Search Results


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  • 93
    Thermo Fisher antibodies against il 27
    Mice lacking <t>IL-27/WSX-1</t> signalling still develop mechanical hypersensitivity after inflammation and peripheral nerve injury. Mice were subjected to the ( a – c) inflammatory or ( d – g ) neuropathic pain model. Fifty percent paw withdrawal threshold before and after ( a ) CFA injection or ( d ) spinal nerve injury. ( b , e ) The percent change in paw withdrawal threshold calculated from a and d, respectively. Each value after CFA injection or nerve injury was normalised by the control value (pre) in each mouse. ( c ) Hind paw weight, as a measure of oedema, in mice 14 days after i.pl. CFA injection. ( f ) Representative immunofluorescence labelling for Iba1 (green) with nuclear staining with TO-PRO-3 (blue) in a transverse section of the dorsal horns from the L4 segment of WT, WSX-1 −/− , EBI3 −/− , and p28 −/− mice 7 days after spinal nerve injury. ( g ) Number of Iba1-positive cells in the ipsilateral or contralateral dorsal horn of L4 spinal segments dissected from WT, WSX-1 −/− , EBI3 −/− , and p28 −/− mice 7 days after spinal nerve injury. n = 5 animals/group. All data are expressed as means ± SEM.
    Antibodies Against Il 27, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against il 27/product/Thermo Fisher
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against il 27 - by Bioz Stars, 2021-09
    93/100 stars
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    90
    Santa Cruz Biotechnology antibodies against il 27
    The effect of <t>IL-27</t> treatment on T lymphocyte differentiation in the spleen after 28 days. A . Representative figures from flow cytometry analyses; CD4 + IL-4 + , CD4 + IL-10 + , CD4 + IL-17 + , CD4 + Foxp3 + and CD4 + IFN-γ + cells from the spleen were analyzed on day 28. B . Analyzed data. Values are expressed as means ± SEM (n = 3). *p
    Antibodies Against Il 27, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies against il 27/product/Santa Cruz Biotechnology
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    antibodies against il 27 - by Bioz Stars, 2021-09
    90/100 stars
      Buy from Supplier

    92
    R&D Systems mabs against human il 27 il 35 ebi3 subunit
    The effect of <t>IL-27</t> treatment on T lymphocyte differentiation in the spleen after 28 days. A . Representative figures from flow cytometry analyses; CD4 + IL-4 + , CD4 + IL-10 + , CD4 + IL-17 + , CD4 + Foxp3 + and CD4 + IFN-γ + cells from the spleen were analyzed on day 28. B . Analyzed data. Values are expressed as means ± SEM (n = 3). *p
    Mabs Against Human Il 27 Il 35 Ebi3 Subunit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mabs against human il 27 il 35 ebi3 subunit/product/R&D Systems
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mabs against human il 27 il 35 ebi3 subunit - by Bioz Stars, 2021-09
    92/100 stars
      Buy from Supplier

    Image Search Results


    Mice lacking IL-27/WSX-1 signalling still develop mechanical hypersensitivity after inflammation and peripheral nerve injury. Mice were subjected to the ( a – c) inflammatory or ( d – g ) neuropathic pain model. Fifty percent paw withdrawal threshold before and after ( a ) CFA injection or ( d ) spinal nerve injury. ( b , e ) The percent change in paw withdrawal threshold calculated from a and d, respectively. Each value after CFA injection or nerve injury was normalised by the control value (pre) in each mouse. ( c ) Hind paw weight, as a measure of oedema, in mice 14 days after i.pl. CFA injection. ( f ) Representative immunofluorescence labelling for Iba1 (green) with nuclear staining with TO-PRO-3 (blue) in a transverse section of the dorsal horns from the L4 segment of WT, WSX-1 −/− , EBI3 −/− , and p28 −/− mice 7 days after spinal nerve injury. ( g ) Number of Iba1-positive cells in the ipsilateral or contralateral dorsal horn of L4 spinal segments dissected from WT, WSX-1 −/− , EBI3 −/− , and p28 −/− mice 7 days after spinal nerve injury. n = 5 animals/group. All data are expressed as means ± SEM.

    Journal: Scientific Reports

    Article Title: Interleukin-27 controls basal pain threshold in physiological and pathological conditions

    doi: 10.1038/s41598-018-29398-3

    Figure Lengend Snippet: Mice lacking IL-27/WSX-1 signalling still develop mechanical hypersensitivity after inflammation and peripheral nerve injury. Mice were subjected to the ( a – c) inflammatory or ( d – g ) neuropathic pain model. Fifty percent paw withdrawal threshold before and after ( a ) CFA injection or ( d ) spinal nerve injury. ( b , e ) The percent change in paw withdrawal threshold calculated from a and d, respectively. Each value after CFA injection or nerve injury was normalised by the control value (pre) in each mouse. ( c ) Hind paw weight, as a measure of oedema, in mice 14 days after i.pl. CFA injection. ( f ) Representative immunofluorescence labelling for Iba1 (green) with nuclear staining with TO-PRO-3 (blue) in a transverse section of the dorsal horns from the L4 segment of WT, WSX-1 −/− , EBI3 −/− , and p28 −/− mice 7 days after spinal nerve injury. ( g ) Number of Iba1-positive cells in the ipsilateral or contralateral dorsal horn of L4 spinal segments dissected from WT, WSX-1 −/− , EBI3 −/− , and p28 −/− mice 7 days after spinal nerve injury. n = 5 animals/group. All data are expressed as means ± SEM.

    Article Snippet: To test whether neutralising antibodies against IL-27 could mimic phenotypes observed in mice lacking IL-27/WSX-1 signalling, WT mice received an i.p. or i.pl. injection of anti-mouse IL-27 (p28) neutralising antibody (1.6 mg/kg or 16 µg/kg, respectively, mouse IgG2a κ isotype, Affymetrix, Santa Clara, CA) or the same dose of an isotype-matched control antibody (mouse IgG2a κ isotype, Affymetrix).

    Techniques: Mouse Assay, Injection, Immunofluorescence, Staining

    Facilitated mechanical sensitivity of C-fibre nociceptors in mice lacking IL-27/WSX-1 signalling. ( a ) Sample recordings of peri-stimulus time histogram. Abscissa: time in seconds. Ordinate: discharge rate of C-nociceptors. Lowest trace: force output of the mechanical stimulus. ( b ) Summarised mechanical response patterns. The response magnitude was significantly greater in WSX-1 −/− , EBI3 −/− , and p28 −/− mice compared with WT. ( a , b ) WT (n = 28), WSX-1 −/− (n = 29), EBI3 −/− (n = 25), p28 −/− (n = 19). P

    Journal: Scientific Reports

    Article Title: Interleukin-27 controls basal pain threshold in physiological and pathological conditions

    doi: 10.1038/s41598-018-29398-3

    Figure Lengend Snippet: Facilitated mechanical sensitivity of C-fibre nociceptors in mice lacking IL-27/WSX-1 signalling. ( a ) Sample recordings of peri-stimulus time histogram. Abscissa: time in seconds. Ordinate: discharge rate of C-nociceptors. Lowest trace: force output of the mechanical stimulus. ( b ) Summarised mechanical response patterns. The response magnitude was significantly greater in WSX-1 −/− , EBI3 −/− , and p28 −/− mice compared with WT. ( a , b ) WT (n = 28), WSX-1 −/− (n = 29), EBI3 −/− (n = 25), p28 −/− (n = 19). P

    Article Snippet: To test whether neutralising antibodies against IL-27 could mimic phenotypes observed in mice lacking IL-27/WSX-1 signalling, WT mice received an i.p. or i.pl. injection of anti-mouse IL-27 (p28) neutralising antibody (1.6 mg/kg or 16 µg/kg, respectively, mouse IgG2a κ isotype, Affymetrix, Santa Clara, CA) or the same dose of an isotype-matched control antibody (mouse IgG2a κ isotype, Affymetrix).

    Techniques: Mouse Assay

    Lack of IL-27/WSX-1 signalling results in enhanced nociceptive behaviours. Reaction latencies to heat measured with the ( a ) hot-plate and ( b ) tail-flick tests. ( c ) Acetone test, in which values represent reaction scores after acetone application to a hind paw. ( d ) Fifty percent paw withdrawal threshold measured with the von Frey test. ( e ) Duration of licking and biting during the first (0–10 min) and second (10–60 min) phases after formalin injection into a hind paw. ( f ) Duration of licking and biting after capsaicin injection into a hind paw. n = 5–10. * P

    Journal: Scientific Reports

    Article Title: Interleukin-27 controls basal pain threshold in physiological and pathological conditions

    doi: 10.1038/s41598-018-29398-3

    Figure Lengend Snippet: Lack of IL-27/WSX-1 signalling results in enhanced nociceptive behaviours. Reaction latencies to heat measured with the ( a ) hot-plate and ( b ) tail-flick tests. ( c ) Acetone test, in which values represent reaction scores after acetone application to a hind paw. ( d ) Fifty percent paw withdrawal threshold measured with the von Frey test. ( e ) Duration of licking and biting during the first (0–10 min) and second (10–60 min) phases after formalin injection into a hind paw. ( f ) Duration of licking and biting after capsaicin injection into a hind paw. n = 5–10. * P

    Article Snippet: To test whether neutralising antibodies against IL-27 could mimic phenotypes observed in mice lacking IL-27/WSX-1 signalling, WT mice received an i.p. or i.pl. injection of anti-mouse IL-27 (p28) neutralising antibody (1.6 mg/kg or 16 µg/kg, respectively, mouse IgG2a κ isotype, Affymetrix, Santa Clara, CA) or the same dose of an isotype-matched control antibody (mouse IgG2a κ isotype, Affymetrix).

    Techniques: Tail Flick Test, Injection

    Intraplantar blockade of IL-27 decreased paw withdrawal threshold in naïve WT mice. Fifty percent paw withdrawal threshold before and after i.pl. injection of a neutralising antibody against p28 or control IgG into WT mice. n = 3–5 animals/group. * P

    Journal: Scientific Reports

    Article Title: Interleukin-27 controls basal pain threshold in physiological and pathological conditions

    doi: 10.1038/s41598-018-29398-3

    Figure Lengend Snippet: Intraplantar blockade of IL-27 decreased paw withdrawal threshold in naïve WT mice. Fifty percent paw withdrawal threshold before and after i.pl. injection of a neutralising antibody against p28 or control IgG into WT mice. n = 3–5 animals/group. * P

    Article Snippet: To test whether neutralising antibodies against IL-27 could mimic phenotypes observed in mice lacking IL-27/WSX-1 signalling, WT mice received an i.p. or i.pl. injection of anti-mouse IL-27 (p28) neutralising antibody (1.6 mg/kg or 16 µg/kg, respectively, mouse IgG2a κ isotype, Affymetrix, Santa Clara, CA) or the same dose of an isotype-matched control antibody (mouse IgG2a κ isotype, Affymetrix).

    Techniques: Mouse Assay, Injection

    IL-27 is an endogenous regulator of thermal and mechanical nociceptive responses. ( a ) Single i.p. injection of rIL-27 dose-dependently prolonged hot-plate latency in EBI3 −/− mice. ( b ) Quick restoration of hot-plate latencies in p28 −/− , but not WSX-1 −/− and WT mice following a single i.p. injection of rIL-27 (16 µg/kg). ( c ) A single i.p. injection of rIL-27 (16 µg/kg) also significantly alleviated mechanical hypersensitivity in EBI3 −/− mice. ( d ) A single i.p. injection of neutralising antibody against p28 (1.6 mg/kg) decreased the paw withdrawal threshold in naïve WT mice. n = 5 animals/group. * P

    Journal: Scientific Reports

    Article Title: Interleukin-27 controls basal pain threshold in physiological and pathological conditions

    doi: 10.1038/s41598-018-29398-3

    Figure Lengend Snippet: IL-27 is an endogenous regulator of thermal and mechanical nociceptive responses. ( a ) Single i.p. injection of rIL-27 dose-dependently prolonged hot-plate latency in EBI3 −/− mice. ( b ) Quick restoration of hot-plate latencies in p28 −/− , but not WSX-1 −/− and WT mice following a single i.p. injection of rIL-27 (16 µg/kg). ( c ) A single i.p. injection of rIL-27 (16 µg/kg) also significantly alleviated mechanical hypersensitivity in EBI3 −/− mice. ( d ) A single i.p. injection of neutralising antibody against p28 (1.6 mg/kg) decreased the paw withdrawal threshold in naïve WT mice. n = 5 animals/group. * P

    Article Snippet: To test whether neutralising antibodies against IL-27 could mimic phenotypes observed in mice lacking IL-27/WSX-1 signalling, WT mice received an i.p. or i.pl. injection of anti-mouse IL-27 (p28) neutralising antibody (1.6 mg/kg or 16 µg/kg, respectively, mouse IgG2a κ isotype, Affymetrix, Santa Clara, CA) or the same dose of an isotype-matched control antibody (mouse IgG2a κ isotype, Affymetrix).

    Techniques: Injection, Mouse Assay

    The effect of IL-27 treatment on T lymphocyte differentiation in the spleen after 28 days. A . Representative figures from flow cytometry analyses; CD4 + IL-4 + , CD4 + IL-10 + , CD4 + IL-17 + , CD4 + Foxp3 + and CD4 + IFN-γ + cells from the spleen were analyzed on day 28. B . Analyzed data. Values are expressed as means ± SEM (n = 3). *p

    Journal: BMC Pulmonary Medicine

    Article Title: IL-27 alleviates the bleomycin-induced pulmonary fibrosis by regulating the Th17 cell differentiation

    doi: 10.1186/s12890-015-0012-4

    Figure Lengend Snippet: The effect of IL-27 treatment on T lymphocyte differentiation in the spleen after 28 days. A . Representative figures from flow cytometry analyses; CD4 + IL-4 + , CD4 + IL-10 + , CD4 + IL-17 + , CD4 + Foxp3 + and CD4 + IFN-γ + cells from the spleen were analyzed on day 28. B . Analyzed data. Values are expressed as means ± SEM (n = 3). *p

    Article Snippet: Antibodies against IL-27 (C-8), COL1A2 (M-19), p-Stat1 (Tyr 701), p-Stat5 (Tyr 694/Tyr 699), p-Smad3 (Ser 208), TGF-βR1 (V-22), p-Stat3 (B-7), and p-Smad1 (Ser 465) were purchased from Santa Cruz Biotechnology.

    Techniques: Flow Cytometry, Cytometry

    IL-27/IL-27R expression increases in bleomycin-induced pulmonary fibrosis. A . Real-time PCR for IL-27 and IL-27R, at days 3, 7, 14, and 28 in the BLM and control groups. The expression of the CT values for real-time PCR were normalized by 2 -∆∆ct . B . IL-27 and IL-27R mRNA expression in the different groups. C , D . Western blot analysis; band intensities were measured using Image J. For each group, n = 3. Data are expressed as the mean ± SEM. *p

    Journal: BMC Pulmonary Medicine

    Article Title: IL-27 alleviates the bleomycin-induced pulmonary fibrosis by regulating the Th17 cell differentiation

    doi: 10.1186/s12890-015-0012-4

    Figure Lengend Snippet: IL-27/IL-27R expression increases in bleomycin-induced pulmonary fibrosis. A . Real-time PCR for IL-27 and IL-27R, at days 3, 7, 14, and 28 in the BLM and control groups. The expression of the CT values for real-time PCR were normalized by 2 -∆∆ct . B . IL-27 and IL-27R mRNA expression in the different groups. C , D . Western blot analysis; band intensities were measured using Image J. For each group, n = 3. Data are expressed as the mean ± SEM. *p

    Article Snippet: Antibodies against IL-27 (C-8), COL1A2 (M-19), p-Stat1 (Tyr 701), p-Stat5 (Tyr 694/Tyr 699), p-Smad3 (Ser 208), TGF-βR1 (V-22), p-Stat3 (B-7), and p-Smad1 (Ser 465) were purchased from Santa Cruz Biotechnology.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot

    Exogenous IL-27 treatment can suppress BLM-induced pulmonary fibrosis. A . Hematoxylin and eosin (HE) staining (100x) and Masson’s trichrome staining (100x) depicting the effects of exogenous IL-27 application on lung histological changes at days 7 and 28 in various treated mice. B . The alveolitis and fibrosis scores for the lungs of mice in the different groups at days 7 and 28. Values are means ± SEM (n = 5). C , D , E . Real-time PCR and Western blot analysis for COL1 and COL3, respectively, in the different groups. CT values for the real-time PCR were normalized by 2 -∆∆ct . Western band intensities were measured using Image J software. For each group, n = 3. F . The lung hydroxyproline content at days 7 and 28 for mice in the various treatment groups. Hydroxyproline content was measured using an ELISA, n = 5. G . Survival rate of mice with BLM-induced pulmonary fibrosis. The percentage of survival over time is presented, n = 5. Data are expressed as means ± SEM. *p

    Journal: BMC Pulmonary Medicine

    Article Title: IL-27 alleviates the bleomycin-induced pulmonary fibrosis by regulating the Th17 cell differentiation

    doi: 10.1186/s12890-015-0012-4

    Figure Lengend Snippet: Exogenous IL-27 treatment can suppress BLM-induced pulmonary fibrosis. A . Hematoxylin and eosin (HE) staining (100x) and Masson’s trichrome staining (100x) depicting the effects of exogenous IL-27 application on lung histological changes at days 7 and 28 in various treated mice. B . The alveolitis and fibrosis scores for the lungs of mice in the different groups at days 7 and 28. Values are means ± SEM (n = 5). C , D , E . Real-time PCR and Western blot analysis for COL1 and COL3, respectively, in the different groups. CT values for the real-time PCR were normalized by 2 -∆∆ct . Western band intensities were measured using Image J software. For each group, n = 3. F . The lung hydroxyproline content at days 7 and 28 for mice in the various treatment groups. Hydroxyproline content was measured using an ELISA, n = 5. G . Survival rate of mice with BLM-induced pulmonary fibrosis. The percentage of survival over time is presented, n = 5. Data are expressed as means ± SEM. *p

    Article Snippet: Antibodies against IL-27 (C-8), COL1A2 (M-19), p-Stat1 (Tyr 701), p-Stat5 (Tyr 694/Tyr 699), p-Smad3 (Ser 208), TGF-βR1 (V-22), p-Stat3 (B-7), and p-Smad1 (Ser 465) were purchased from Santa Cruz Biotechnology.

    Techniques: Staining, Mouse Assay, Real-time Polymerase Chain Reaction, Western Blot, Software, Enzyme-linked Immunosorbent Assay