antibodies against caspase-9 Search Results


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  • 99
    Cell Signaling Technology Inc antibodies against caspase 9
    Apoptosis induced during later stages of rotavirus infection (SA11) is partly dependent on p53 expression. (A and B) Inhibition of p53 function during SA11 infection inhibits upregulation of the PUMA level and Bax activation. MA104 cells were transfected with pCMV-p53-mt135 (A) or a nonspecific vector, pACGFP (B), for 24 h and then infected with SA11 for the indicated time points, followed by immunoblot analysis of whole-cell lysates (anti-PUMA, anti-β-actin) and the mitochondrial fraction (anti-Bax, anti-Cox4). (C and D) In the absence of functional p53, rotavirus-induced caspase activation was decreased. MA104 cells were either infected with SA11 in the presence or absence of <t>caspase-9</t> inhibitor benzyloxycarbonyl-Leu-Glu-His-Asp-methyl-fluoromethylketone (Z-LEHD-FMK) (C) or caspase-3 inhibitor benzyloxycarbonyl-Asp-Glu-Val-Asp-fluoromethylketone (Z-DEVD-FMK) (D) or transfected with pCMV-p53-mt135 or a nonspecific vector, pACGFP, for 24 h and then infected with SA11 for the indicated time points, followed by caspase activity assay using synthetic substrates for caspase-9 (LEHD-AMC) and caspase-3 (DEVD-AFC). Results are representative of three independent experiments. Values represent the means ± SDs of one experiment with three measurements taken.
    Antibodies Against Caspase 9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 148 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc primary antibodies against caspase 9
    Functional antagonism of Smac mimetics to XIAP protein (residues 120–356) in cell-free ( A ) <t>caspase-9</t> and ( B ) caspase-3/-7 activity assays.
    Primary Antibodies Against Caspase 9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam antibodies against caspase 9
    Upregulation of Btg2 and <t>caspase</t> 9 by RXR ligands is not affected by an RAR antagonist
    Antibodies Against Caspase 9, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology antibodies against caspase 9
    Elevation of Srx-1 attenuated SI/R-triggered mitochondrial apoptosis pathway After transfection with Ad-Srx-1, cells were then exposure to SI/R. ( A ) Following staining with rhodamine 123 reagent, the loss of Δ ψ m was analysed by flow cytometry. ( B ) The specific substrates of DEVD-AFC (for caspase-3) or LEHD-AMC (for <t>caspase-9)</t> were introduced, and the caspase-9 and caspase-3 activity was assessed by fluorimetry. ( C ) Western blotting was performed to detect the expression of cytochrome c , caspase-9, caspase-3, PARP, Bax and Bcl-2. ( D ) Densitometry of western blot band intensities by Quantity One. * P
    Antibodies Against Caspase 9, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 89/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc antibodies against cleaved caspase 9
    AWRK6 inhibited LPS-induced liver cell apoptosis in HepG2 cells. ( A ) The viabilities of HepG2 liver cells treated with LPS (40 μg/mL) with/without AWRK6 for 24 h, examined by MTT assay. ( B ) The cells treated with LPS and AWRK6 (200 μg/mL) were observed under phase contrast microscope. ( C ) The cell apoptosis was detected by Annexin V-FITC/PI staining followed by fluorescence microscopy. ( D ) The apoptotic cell number in the results of Annexin V-FITC/PI staining was analyzed by ImageJ. ( E ) The protein levels of cleaved-caspase 9, BAX and Bcl-2 were analyzed by western blotting. ( F ) The results of western blotting were quantified using ImageJ. Bar indicates 100 μm. *  p
    Antibodies Against Cleaved Caspase 9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Cell Signaling Technology Inc rabbit polyclonal antibody against caspase 9
    AWRK6 inhibited LPS-induced liver cell apoptosis in HepG2 cells. ( A ) The viabilities of HepG2 liver cells treated with LPS (40 μg/mL) with/without AWRK6 for 24 h, examined by MTT assay. ( B ) The cells treated with LPS and AWRK6 (200 μg/mL) were observed under phase contrast microscope. ( C ) The cell apoptosis was detected by Annexin V-FITC/PI staining followed by fluorescence microscopy. ( D ) The apoptotic cell number in the results of Annexin V-FITC/PI staining was analyzed by ImageJ. ( E ) The protein levels of cleaved-caspase 9, BAX and Bcl-2 were analyzed by western blotting. ( F ) The results of western blotting were quantified using ImageJ. Bar indicates 100 μm. *  p
    Rabbit Polyclonal Antibody Against Caspase 9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 89/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology rabbit polyclonal antibody against caspase 9
    AWRK6 inhibited LPS-induced liver cell apoptosis in HepG2 cells. ( A ) The viabilities of HepG2 liver cells treated with LPS (40 μg/mL) with/without AWRK6 for 24 h, examined by MTT assay. ( B ) The cells treated with LPS and AWRK6 (200 μg/mL) were observed under phase contrast microscope. ( C ) The cell apoptosis was detected by Annexin V-FITC/PI staining followed by fluorescence microscopy. ( D ) The apoptotic cell number in the results of Annexin V-FITC/PI staining was analyzed by ImageJ. ( E ) The protein levels of cleaved-caspase 9, BAX and Bcl-2 were analyzed by western blotting. ( F ) The results of western blotting were quantified using ImageJ. Bar indicates 100 μm. *  p
    Rabbit Polyclonal Antibody Against Caspase 9, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 89/100, based on 109 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Epitomics antibodies against caspase 9
    AWRK6 inhibited LPS-induced liver cell apoptosis in HepG2 cells. ( A ) The viabilities of HepG2 liver cells treated with LPS (40 μg/mL) with/without AWRK6 for 24 h, examined by MTT assay. ( B ) The cells treated with LPS and AWRK6 (200 μg/mL) were observed under phase contrast microscope. ( C ) The cell apoptosis was detected by Annexin V-FITC/PI staining followed by fluorescence microscopy. ( D ) The apoptotic cell number in the results of Annexin V-FITC/PI staining was analyzed by ImageJ. ( E ) The protein levels of cleaved-caspase 9, BAX and Bcl-2 were analyzed by western blotting. ( F ) The results of western blotting were quantified using ImageJ. Bar indicates 100 μm. *  p
    Antibodies Against Caspase 9, supplied by Epitomics, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam rabbit polyclonal antibody against caspase 9
    AWRK6 inhibited LPS-induced liver cell apoptosis in HepG2 cells. ( A ) The viabilities of HepG2 liver cells treated with LPS (40 μg/mL) with/without AWRK6 for 24 h, examined by MTT assay. ( B ) The cells treated with LPS and AWRK6 (200 μg/mL) were observed under phase contrast microscope. ( C ) The cell apoptosis was detected by Annexin V-FITC/PI staining followed by fluorescence microscopy. ( D ) The apoptotic cell number in the results of Annexin V-FITC/PI staining was analyzed by ImageJ. ( E ) The protein levels of cleaved-caspase 9, BAX and Bcl-2 were analyzed by western blotting. ( F ) The results of western blotting were quantified using ImageJ. Bar indicates 100 μm. *  p
    Rabbit Polyclonal Antibody Against Caspase 9, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology mouse monoclonal antibody against caspase 9
    HOCl-MSA promoted binding of apaf-1 and caspase 9, which was prevented by SS-31 in cultured podocyte. Data are expressed as Mean ± SD. ANOVA,  p
    Mouse Monoclonal Antibody Against Caspase 9, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam primary antibodies against caspase 9
    RS downregulates <t>caspase-9</t> and cyt c expression, and upregulates UCP2 and PPAR-γ expression. A total of 48 rabbits were randomly divided into four groups, including the sham group, MI/R group, RS group and RS + ATR group. mRNA and protein expression levels of caspase-9, cyt c, UCP2 and PPAR-γ were detected by (A) reverse transcription-quantitative polymerase chain reaction and (B) western blotting. (C) The results of western blotting were quantitatively analyzed. ***P
    Primary Antibodies Against Caspase 9, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc antibodies against caspase 3 7 8 9
    RS downregulates <t>caspase-9</t> and cyt c expression, and upregulates UCP2 and PPAR-γ expression. A total of 48 rabbits were randomly divided into four groups, including the sham group, MI/R group, RS group and RS + ATR group. mRNA and protein expression levels of caspase-9, cyt c, UCP2 and PPAR-γ were detected by (A) reverse transcription-quantitative polymerase chain reaction and (B) western blotting. (C) The results of western blotting were quantitatively analyzed. ***P
    Antibodies Against Caspase 3 7 8 9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc antibodies against caspase 3
    Effect of FA-DM1-NPs and S-DM1 on expression levels of apoptosis-related proteins in MCF-7/HER2 cells. Following the treatment of breast cancer cells with therapeutic concentrations of drugs (5.2 µg/mL S-DM1 and 10 µg/mL FA-DM1-NPs equivalent DM1 5.0 µg/mL, respectively), the expression levels of <t>procaspase-3/-8/-9</t> and Bcl-2 were analyzed by Western blot (A) and Gel-Pro Analyzer 4.0 software (B) . ** p
    Antibodies Against Caspase 3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 1029 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Assay Designs Inc antibodies against caspase 9
    Effect of FA-DM1-NPs and S-DM1 on expression levels of apoptosis-related proteins in MCF-7/HER2 cells. Following the treatment of breast cancer cells with therapeutic concentrations of drugs (5.2 µg/mL S-DM1 and 10 µg/mL FA-DM1-NPs equivalent DM1 5.0 µg/mL, respectively), the expression levels of <t>procaspase-3/-8/-9</t> and Bcl-2 were analyzed by Western blot (A) and Gel-Pro Analyzer 4.0 software (B) . ** p
    Antibodies Against Caspase 9, supplied by Assay Designs Inc, used in various techniques. Bioz Stars score: 89/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson antibodies against caspase 9
    CDK1 mediates paclitaxel-induced chemoresistance through <t>caspase-9</t> ( A ) Total caspase-9 and phospho-caspase-9 levels were analyzed by western blot. Actin was used as a loading control. CS, chemosenstive; CR, chemoresistant. ( B ) HeyA8-MDR and SKOV3-TR cells were treated with nocodazole to arrest cells in their mitosis. Noco, nocodazole; Als, alsterpaullone; PTX, paclitaxel. ( C ) Cell death was compared between three experimental groups: alsterpaullone-pretreated cells (upper left), alsterpaullonepretreated cells with added nocodazole (upper right), and alsterpaullone-pretreated cells with added paclitaxel and nocodazole (lower left). Administration of paclitaxel was followed by nocodazole treatment. ( D ) Populations of adherent (blue) and floating (green) cells are shown. Dead cells were stained with red dye.
    Antibodies Against Caspase 9, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MBL International antibodies against caspase 9
    tRNA block apoptosome formation and <t>caspase-9</t> activation
    Antibodies Against Caspase 9, supplied by MBL International, used in various techniques. Bioz Stars score: 89/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam antibody against murine caspase 9
    Effects of putative MCL-1 inhibitors on Bax/Bak- and <t>caspase-9-deficient</t> MEFs. MEFs deficient in either Bax and Bak (dotted lines) or caspase-9 (dashed lines) along with their wild-type counterparts (continuous bold lines) were exposed for 24 h
    Antibody Against Murine Caspase 9, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology polyclonal antibody against caspase 9 p18 subunit
    Effects of putative MCL-1 inhibitors on Bax/Bak- and <t>caspase-9-deficient</t> MEFs. MEFs deficient in either Bax and Bak (dotted lines) or caspase-9 (dashed lines) along with their wild-type counterparts (continuous bold lines) were exposed for 24 h
    Polyclonal Antibody Against Caspase 9 P18 Subunit, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Novus Biologicals polyclonal antibody against caspase 9
    Effects of putative MCL-1 inhibitors on Bax/Bak- and <t>caspase-9-deficient</t> MEFs. MEFs deficient in either Bax and Bak (dotted lines) or caspase-9 (dashed lines) along with their wild-type counterparts (continuous bold lines) were exposed for 24 h
    Polyclonal Antibody Against Caspase 9, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc polyclonal antibodies against procaspase cleaved caspase 9
    Effects of putative MCL-1 inhibitors on Bax/Bak- and <t>caspase-9-deficient</t> MEFs. MEFs deficient in either Bax and Bak (dotted lines) or caspase-9 (dashed lines) along with their wild-type counterparts (continuous bold lines) were exposed for 24 h
    Polyclonal Antibodies Against Procaspase Cleaved Caspase 9, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    WuXi AppTec rabbit polyclonal antibody against phospho caspase 9 ser196
    Effects of putative MCL-1 inhibitors on Bax/Bak- and <t>caspase-9-deficient</t> MEFs. MEFs deficient in either Bax and Bak (dotted lines) or caspase-9 (dashed lines) along with their wild-type counterparts (continuous bold lines) were exposed for 24 h
    Rabbit Polyclonal Antibody Against Phospho Caspase 9 Ser196, supplied by WuXi AppTec, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Apoptosis induced during later stages of rotavirus infection (SA11) is partly dependent on p53 expression. (A and B) Inhibition of p53 function during SA11 infection inhibits upregulation of the PUMA level and Bax activation. MA104 cells were transfected with pCMV-p53-mt135 (A) or a nonspecific vector, pACGFP (B), for 24 h and then infected with SA11 for the indicated time points, followed by immunoblot analysis of whole-cell lysates (anti-PUMA, anti-β-actin) and the mitochondrial fraction (anti-Bax, anti-Cox4). (C and D) In the absence of functional p53, rotavirus-induced caspase activation was decreased. MA104 cells were either infected with SA11 in the presence or absence of caspase-9 inhibitor benzyloxycarbonyl-Leu-Glu-His-Asp-methyl-fluoromethylketone (Z-LEHD-FMK) (C) or caspase-3 inhibitor benzyloxycarbonyl-Asp-Glu-Val-Asp-fluoromethylketone (Z-DEVD-FMK) (D) or transfected with pCMV-p53-mt135 or a nonspecific vector, pACGFP, for 24 h and then infected with SA11 for the indicated time points, followed by caspase activity assay using synthetic substrates for caspase-9 (LEHD-AMC) and caspase-3 (DEVD-AFC). Results are representative of three independent experiments. Values represent the means ± SDs of one experiment with three measurements taken.

    Journal: Journal of Virology

    Article Title: Rotavirus-Encoded Nonstructural Protein 1 Modulates Cellular Apoptotic Machinery by Targeting Tumor Suppressor Protein p53

    doi: 10.1128/JVI.00734-13

    Figure Lengend Snippet: Apoptosis induced during later stages of rotavirus infection (SA11) is partly dependent on p53 expression. (A and B) Inhibition of p53 function during SA11 infection inhibits upregulation of the PUMA level and Bax activation. MA104 cells were transfected with pCMV-p53-mt135 (A) or a nonspecific vector, pACGFP (B), for 24 h and then infected with SA11 for the indicated time points, followed by immunoblot analysis of whole-cell lysates (anti-PUMA, anti-β-actin) and the mitochondrial fraction (anti-Bax, anti-Cox4). (C and D) In the absence of functional p53, rotavirus-induced caspase activation was decreased. MA104 cells were either infected with SA11 in the presence or absence of caspase-9 inhibitor benzyloxycarbonyl-Leu-Glu-His-Asp-methyl-fluoromethylketone (Z-LEHD-FMK) (C) or caspase-3 inhibitor benzyloxycarbonyl-Asp-Glu-Val-Asp-fluoromethylketone (Z-DEVD-FMK) (D) or transfected with pCMV-p53-mt135 or a nonspecific vector, pACGFP, for 24 h and then infected with SA11 for the indicated time points, followed by caspase activity assay using synthetic substrates for caspase-9 (LEHD-AMC) and caspase-3 (DEVD-AFC). Results are representative of three independent experiments. Values represent the means ± SDs of one experiment with three measurements taken.

    Article Snippet: Antibodies against caspase-9 (catalog no. 9501 and 9502), caspase-3 (catalog no. 9662 and 9664), poly(ADP-ribose) polymerase (PARP (catalog no. 9541 and 9542), Cox4 (catalog no. 4844), GAPDH (glyceraldehyde-3-phosphate dehydrogenase; catalog no. 2118), PUMA (catalog no. 4976), and β-actin (catalog no. 4967) were from Cell Signaling Technology.

    Techniques: Infection, Expressing, Inhibition, Activation Assay, Transfection, Plasmid Preparation, Functional Assay, Caspase Activity Assay

    Functional antagonism of Smac mimetics to XIAP protein (residues 120–356) in cell-free ( A ) caspase-9 and ( B ) caspase-3/-7 activity assays.

    Journal: Journal of the American Chemical Society

    Article Title: Design, Synthesis and Characterization of A Potent, Non-Peptide, Cell-Permeable, Bivalent Smac Mimetic that Concurrently Targets both the BIR2 and BIR3 Domains in XIAP

    doi: 10.1021/ja074725f

    Figure Lengend Snippet: Functional antagonism of Smac mimetics to XIAP protein (residues 120–356) in cell-free ( A ) caspase-9 and ( B ) caspase-3/-7 activity assays.

    Article Snippet: Primary antibodies against caspase-9, cleaved caspase 3 and β-actin were purchased from Cell Signaling Technology; primary antibody against cleaved PARP was purchased from Epitomics.

    Techniques: Functional Assay, Activity Assay

    ( A ). A schematic, simplified apoptosis pathway. XIAP inhibits apoptosis by directly binding to and inhibition of caspase-9, caspase-3 and -7. Smac protein binds to XIAP and antagonizes XIAP to promote activation of caspases and apoptosis. ( B ). Design

    Journal: Journal of the American Chemical Society

    Article Title: Design, Synthesis and Characterization of A Potent, Non-Peptide, Cell-Permeable, Bivalent Smac Mimetic that Concurrently Targets both the BIR2 and BIR3 Domains in XIAP

    doi: 10.1021/ja074725f

    Figure Lengend Snippet: ( A ). A schematic, simplified apoptosis pathway. XIAP inhibits apoptosis by directly binding to and inhibition of caspase-9, caspase-3 and -7. Smac protein binds to XIAP and antagonizes XIAP to promote activation of caspases and apoptosis. ( B ). Design

    Article Snippet: Primary antibodies against caspase-9, cleaved caspase 3 and β-actin were purchased from Cell Signaling Technology; primary antibody against cleaved PARP was purchased from Epitomics.

    Techniques: Binding Assay, Inhibition, Activation Assay

    Upregulation of Btg2 and caspase 9 by RXR ligands is not affected by an RAR antagonist

    Journal: Journal of molecular biology

    Article Title: INHIBITION OF MAMMARY CARCINOMA CELL GROWTH BY RXR IS MEDIATED BY THE RECEPTOR'S OLIGOMERIC SWITCH

    doi: 10.1016/j.jmb.2010.02.030

    Figure Lengend Snippet: Upregulation of Btg2 and caspase 9 by RXR ligands is not affected by an RAR antagonist

    Article Snippet: Antibodies against caspase 9 and β-tubulin were obtained from Abcam (Cambridge, MA) and Sigma Chemical Co., respectively.

    Techniques:

    An RXR antagonist induces tetramer dissociation and upregulates Btg2 and caspase 9 expression

    Journal: Journal of molecular biology

    Article Title: INHIBITION OF MAMMARY CARCINOMA CELL GROWTH BY RXR IS MEDIATED BY THE RECEPTOR'S OLIGOMERIC SWITCH

    doi: 10.1016/j.jmb.2010.02.030

    Figure Lengend Snippet: An RXR antagonist induces tetramer dissociation and upregulates Btg2 and caspase 9 expression

    Article Snippet: Antibodies against caspase 9 and β-tubulin were obtained from Abcam (Cambridge, MA) and Sigma Chemical Co., respectively.

    Techniques: Expressing

    Expression of Btg2 and caspase 9 is regulated by the tetramer/dimer switch of RXR

    Journal: Journal of molecular biology

    Article Title: INHIBITION OF MAMMARY CARCINOMA CELL GROWTH BY RXR IS MEDIATED BY THE RECEPTOR'S OLIGOMERIC SWITCH

    doi: 10.1016/j.jmb.2010.02.030

    Figure Lengend Snippet: Expression of Btg2 and caspase 9 is regulated by the tetramer/dimer switch of RXR

    Article Snippet: Antibodies against caspase 9 and β-tubulin were obtained from Abcam (Cambridge, MA) and Sigma Chemical Co., respectively.

    Techniques: Expressing

    Elevation of Srx-1 attenuated SI/R-triggered mitochondrial apoptosis pathway After transfection with Ad-Srx-1, cells were then exposure to SI/R. ( A ) Following staining with rhodamine 123 reagent, the loss of Δ ψ m was analysed by flow cytometry. ( B ) The specific substrates of DEVD-AFC (for caspase-3) or LEHD-AMC (for caspase-9) were introduced, and the caspase-9 and caspase-3 activity was assessed by fluorimetry. ( C ) Western blotting was performed to detect the expression of cytochrome c , caspase-9, caspase-3, PARP, Bax and Bcl-2. ( D ) Densitometry of western blot band intensities by Quantity One. * P

    Journal: Bioscience Reports

    Article Title: Sulfiredoxin-1 protects against simulated ischaemia/reperfusion injury in cardiomyocyte by inhibiting PI3K/AKT-regulated mitochondrial apoptotic pathways

    doi: 10.1042/BSR20160076

    Figure Lengend Snippet: Elevation of Srx-1 attenuated SI/R-triggered mitochondrial apoptosis pathway After transfection with Ad-Srx-1, cells were then exposure to SI/R. ( A ) Following staining with rhodamine 123 reagent, the loss of Δ ψ m was analysed by flow cytometry. ( B ) The specific substrates of DEVD-AFC (for caspase-3) or LEHD-AMC (for caspase-9) were introduced, and the caspase-9 and caspase-3 activity was assessed by fluorimetry. ( C ) Western blotting was performed to detect the expression of cytochrome c , caspase-9, caspase-3, PARP, Bax and Bcl-2. ( D ) Densitometry of western blot band intensities by Quantity One. * P

    Article Snippet: The antibodies against caspase-9, Bax and Bcl-2 were acquired from Santa Cruz Biotechnology.

    Techniques: Transfection, Staining, Flow Cytometry, Cytometry, Activity Assay, Western Blot, Expressing

    Involvement of the PI3K/AKT pathway in Srx-1-mediated cardiomyocyte resistance to mitochondrial apoptotic pathway triggered by SI/R condition Before transfection with Ad-Srx-1, H9c2 cells were pretreated with LY294002. Then, cells were incubated under 10 h hypoxia and 3 h reoxygenation (SI/R). ( A ) The effect on Δ ψ m loss was analysed by flow cytometry. ( B ) The activity of caspase-9 and caspase-3 were evaluated by a fluorometer. ( C ) Western blotting assessed the expression of cytochrome c , Bax and Bcl-2. ( D ) The corresponding quantitative analysis of Bcl-2 protein. ( E ) The quantified band intensities of cytochrome c and Bax. * P

    Journal: Bioscience Reports

    Article Title: Sulfiredoxin-1 protects against simulated ischaemia/reperfusion injury in cardiomyocyte by inhibiting PI3K/AKT-regulated mitochondrial apoptotic pathways

    doi: 10.1042/BSR20160076

    Figure Lengend Snippet: Involvement of the PI3K/AKT pathway in Srx-1-mediated cardiomyocyte resistance to mitochondrial apoptotic pathway triggered by SI/R condition Before transfection with Ad-Srx-1, H9c2 cells were pretreated with LY294002. Then, cells were incubated under 10 h hypoxia and 3 h reoxygenation (SI/R). ( A ) The effect on Δ ψ m loss was analysed by flow cytometry. ( B ) The activity of caspase-9 and caspase-3 were evaluated by a fluorometer. ( C ) Western blotting assessed the expression of cytochrome c , Bax and Bcl-2. ( D ) The corresponding quantitative analysis of Bcl-2 protein. ( E ) The quantified band intensities of cytochrome c and Bax. * P

    Article Snippet: The antibodies against caspase-9, Bax and Bcl-2 were acquired from Santa Cruz Biotechnology.

    Techniques: Transfection, Incubation, Flow Cytometry, Cytometry, Activity Assay, Western Blot, Expressing

    AWRK6 inhibited LPS-induced liver cell apoptosis in HepG2 cells. ( A ) The viabilities of HepG2 liver cells treated with LPS (40 μg/mL) with/without AWRK6 for 24 h, examined by MTT assay. ( B ) The cells treated with LPS and AWRK6 (200 μg/mL) were observed under phase contrast microscope. ( C ) The cell apoptosis was detected by Annexin V-FITC/PI staining followed by fluorescence microscopy. ( D ) The apoptotic cell number in the results of Annexin V-FITC/PI staining was analyzed by ImageJ. ( E ) The protein levels of cleaved-caspase 9, BAX and Bcl-2 were analyzed by western blotting. ( F ) The results of western blotting were quantified using ImageJ. Bar indicates 100 μm. *  p

    Journal: International Journal of Molecular Sciences

    Article Title: A Synthetic Peptide AWRK6 Alleviates Lipopolysaccharide-Induced Liver Injury

    doi: 10.3390/ijms19092661

    Figure Lengend Snippet: AWRK6 inhibited LPS-induced liver cell apoptosis in HepG2 cells. ( A ) The viabilities of HepG2 liver cells treated with LPS (40 μg/mL) with/without AWRK6 for 24 h, examined by MTT assay. ( B ) The cells treated with LPS and AWRK6 (200 μg/mL) were observed under phase contrast microscope. ( C ) The cell apoptosis was detected by Annexin V-FITC/PI staining followed by fluorescence microscopy. ( D ) The apoptotic cell number in the results of Annexin V-FITC/PI staining was analyzed by ImageJ. ( E ) The protein levels of cleaved-caspase 9, BAX and Bcl-2 were analyzed by western blotting. ( F ) The results of western blotting were quantified using ImageJ. Bar indicates 100 μm. * p

    Article Snippet: The membranes were incubated overnight at 4 °C with antibodies against cleaved-caspase 9 (1:2000, 9509s, CST, Shanghai, China), Bax (1:2000, TA810334, OriGene, Beijing, China), Bcl-2 (1:2000, UM870117, OriGene), GAPDH (1:8000, TA-08, ZSGB Bio, Beijing, China), phospho-ERK (1:1500, 4370T, CST), ERK (1:1500, 4695T, CST), phosphor-JNK (1:1500, 4668T, CST), JNK (1:1500, 9252T, CST), phosphor-p38 MAPK (1:1500, 4511T, CST) and p38 MAPK (1:1500, 8690T, CST), followed by incubation with HRP-conjugated secondary antibodies (1:8000, ZDR-5306 and ZDR-5307, ZSGB Bio).

    Techniques: MTT Assay, Microscopy, Staining, Fluorescence, Western Blot

    AWRK6 inhibited LPS-induced apoptosis in mice liver. ( A ) AWRK6 (10 mg/kg) treatment for 24 h reduced DNA fragmentation induced by LPS (50 mg/kg), assayed by TUNEL assay. ( B ) The results of TUNEL assay were analyzed by ImageJ. ( C ) The protein levels of cleaved-caspase 9, BAX and Bcl-2 were analyzed by western blotting. ( D ) The quantification of western blotting results was carried out using ImageJ. *  p

    Journal: International Journal of Molecular Sciences

    Article Title: A Synthetic Peptide AWRK6 Alleviates Lipopolysaccharide-Induced Liver Injury

    doi: 10.3390/ijms19092661

    Figure Lengend Snippet: AWRK6 inhibited LPS-induced apoptosis in mice liver. ( A ) AWRK6 (10 mg/kg) treatment for 24 h reduced DNA fragmentation induced by LPS (50 mg/kg), assayed by TUNEL assay. ( B ) The results of TUNEL assay were analyzed by ImageJ. ( C ) The protein levels of cleaved-caspase 9, BAX and Bcl-2 were analyzed by western blotting. ( D ) The quantification of western blotting results was carried out using ImageJ. * p

    Article Snippet: The membranes were incubated overnight at 4 °C with antibodies against cleaved-caspase 9 (1:2000, 9509s, CST, Shanghai, China), Bax (1:2000, TA810334, OriGene, Beijing, China), Bcl-2 (1:2000, UM870117, OriGene), GAPDH (1:8000, TA-08, ZSGB Bio, Beijing, China), phospho-ERK (1:1500, 4370T, CST), ERK (1:1500, 4695T, CST), phosphor-JNK (1:1500, 4668T, CST), JNK (1:1500, 9252T, CST), phosphor-p38 MAPK (1:1500, 4511T, CST) and p38 MAPK (1:1500, 8690T, CST), followed by incubation with HRP-conjugated secondary antibodies (1:8000, ZDR-5306 and ZDR-5307, ZSGB Bio).

    Techniques: Mouse Assay, TUNEL Assay, Western Blot

    HOCl-MSA promoted binding of apaf-1 and caspase 9, which was prevented by SS-31 in cultured podocyte. Data are expressed as Mean ± SD. ANOVA,  p

    Journal: Journal of Enzyme Inhibition and Medicinal Chemistry

    Article Title: A mitochondrial-targeted peptide ameliorated podocyte apoptosis through a HOCl-alb-enhanced and mitochondria-dependent signalling pathway in diabetic rats and in vitro

    doi: 10.1080/14756366.2018.1488697

    Figure Lengend Snippet: HOCl-MSA promoted binding of apaf-1 and caspase 9, which was prevented by SS-31 in cultured podocyte. Data are expressed as Mean ± SD. ANOVA, p

    Article Snippet: In brief, the cell lysates were preabsorbed with protein A/G agarose beads (Santa Cruz Biotechnology) and incubated with a mouse monoclonal antibody against caspase-9 (2 μg; Santa Cruz Biotechnology).

    Techniques: Binding Assay, Cell Culture

    RS downregulates caspase-9 and cyt c expression, and upregulates UCP2 and PPAR-γ expression. A total of 48 rabbits were randomly divided into four groups, including the sham group, MI/R group, RS group and RS + ATR group. mRNA and protein expression levels of caspase-9, cyt c, UCP2 and PPAR-γ were detected by (A) reverse transcription-quantitative polymerase chain reaction and (B) western blotting. (C) The results of western blotting were quantitatively analyzed. ***P

    Journal: Molecular Medicine Reports

    Article Title: Rosuvastatin relieves myocardial ischemia/reperfusion injury by upregulating PPAR-γ and UCP2

    doi: 10.3892/mmr.2018.9062

    Figure Lengend Snippet: RS downregulates caspase-9 and cyt c expression, and upregulates UCP2 and PPAR-γ expression. A total of 48 rabbits were randomly divided into four groups, including the sham group, MI/R group, RS group and RS + ATR group. mRNA and protein expression levels of caspase-9, cyt c, UCP2 and PPAR-γ were detected by (A) reverse transcription-quantitative polymerase chain reaction and (B) western blotting. (C) The results of western blotting were quantitatively analyzed. ***P

    Article Snippet: Proteins were blocked with 5% skimmed milk at room temperature for 2 h. Primary antibodies against caspase-9 (1:5,000; cat. no. ab2324), cyt c (1:5,000; cat. no. ab28146), UCP2 (1:1,000; cat. no. ab97931), PPAR-γ (1:1,000; cat. no. ab223137) and GAPDH (1:2,500; cat. no. ab9485) were from Abcam (Cambridge, MA) and were incubated with the membranes at 4°C overnight.

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot

    RS downregulates protein expression levels of caspase-9 and cyt c, and upregulates protein expression levels of UCP2 and PPAR-γ following OGD/R damage. Cardiomyocytes were divided into the following groups: Control, control + siPPAR-γ, OGD/R, OGD/R + siPPAR-γ, RS + OGD/R, RS + OGD/R + siPPAR-γ, ATR + RS + OGD/R, ATR + RS + OGD/R + siPPAR-γ. (A) Western blot analysis was used to detect the protein expression levels of caspase-9, cyt c, UCP2 and PPAR-γ in cardiomyocytes. (B) The protein expression levels were quantitatively analyzed according to the protein gray values. **P

    Journal: Molecular Medicine Reports

    Article Title: Rosuvastatin relieves myocardial ischemia/reperfusion injury by upregulating PPAR-γ and UCP2

    doi: 10.3892/mmr.2018.9062

    Figure Lengend Snippet: RS downregulates protein expression levels of caspase-9 and cyt c, and upregulates protein expression levels of UCP2 and PPAR-γ following OGD/R damage. Cardiomyocytes were divided into the following groups: Control, control + siPPAR-γ, OGD/R, OGD/R + siPPAR-γ, RS + OGD/R, RS + OGD/R + siPPAR-γ, ATR + RS + OGD/R, ATR + RS + OGD/R + siPPAR-γ. (A) Western blot analysis was used to detect the protein expression levels of caspase-9, cyt c, UCP2 and PPAR-γ in cardiomyocytes. (B) The protein expression levels were quantitatively analyzed according to the protein gray values. **P

    Article Snippet: Proteins were blocked with 5% skimmed milk at room temperature for 2 h. Primary antibodies against caspase-9 (1:5,000; cat. no. ab2324), cyt c (1:5,000; cat. no. ab28146), UCP2 (1:1,000; cat. no. ab97931), PPAR-γ (1:1,000; cat. no. ab223137) and GAPDH (1:2,500; cat. no. ab9485) were from Abcam (Cambridge, MA) and were incubated with the membranes at 4°C overnight.

    Techniques: Expressing, Western Blot

    Pretreatment with RS decreases caspase-9 and cyt c mRNA expression, and increases UCP2 and PPAR-γ mRNA expression following OGD/R damage. Cardiomyocytes were divided into the following groups: Control, control + siPPAR-γ, OGD/R, OGD/R + siPPAR-γ, RS + OGD/R, RS + OGD/R + siPPAR-γ, ATR + RS + OGD/R, ATR + RS + OGD/R + siPPAR-γ. Reverse transcription-quantitative polymerase chain reaction was performed to analyze the mRNA expression levels of (A) caspase-9, (B) cyt c, (C) UCP2 and (D) PPAR-γ in cardiomyocytes. *P

    Journal: Molecular Medicine Reports

    Article Title: Rosuvastatin relieves myocardial ischemia/reperfusion injury by upregulating PPAR-γ and UCP2

    doi: 10.3892/mmr.2018.9062

    Figure Lengend Snippet: Pretreatment with RS decreases caspase-9 and cyt c mRNA expression, and increases UCP2 and PPAR-γ mRNA expression following OGD/R damage. Cardiomyocytes were divided into the following groups: Control, control + siPPAR-γ, OGD/R, OGD/R + siPPAR-γ, RS + OGD/R, RS + OGD/R + siPPAR-γ, ATR + RS + OGD/R, ATR + RS + OGD/R + siPPAR-γ. Reverse transcription-quantitative polymerase chain reaction was performed to analyze the mRNA expression levels of (A) caspase-9, (B) cyt c, (C) UCP2 and (D) PPAR-γ in cardiomyocytes. *P

    Article Snippet: Proteins were blocked with 5% skimmed milk at room temperature for 2 h. Primary antibodies against caspase-9 (1:5,000; cat. no. ab2324), cyt c (1:5,000; cat. no. ab28146), UCP2 (1:1,000; cat. no. ab97931), PPAR-γ (1:1,000; cat. no. ab223137) and GAPDH (1:2,500; cat. no. ab9485) were from Abcam (Cambridge, MA) and were incubated with the membranes at 4°C overnight.

    Techniques: Expressing, Real-time Polymerase Chain Reaction

    Effect of FA-DM1-NPs and S-DM1 on expression levels of apoptosis-related proteins in MCF-7/HER2 cells. Following the treatment of breast cancer cells with therapeutic concentrations of drugs (5.2 µg/mL S-DM1 and 10 µg/mL FA-DM1-NPs equivalent DM1 5.0 µg/mL, respectively), the expression levels of procaspase-3/-8/-9 and Bcl-2 were analyzed by Western blot (A) and Gel-Pro Analyzer 4.0 software (B) . ** p

    Journal: Nanoscale Research Letters

    Article Title: Enhanced anticancer activity of DM1-loaded star-shaped folate-core PLA-TPGS nanoparticles

    doi: 10.1186/1556-276X-9-563

    Figure Lengend Snippet: Effect of FA-DM1-NPs and S-DM1 on expression levels of apoptosis-related proteins in MCF-7/HER2 cells. Following the treatment of breast cancer cells with therapeutic concentrations of drugs (5.2 µg/mL S-DM1 and 10 µg/mL FA-DM1-NPs equivalent DM1 5.0 µg/mL, respectively), the expression levels of procaspase-3/-8/-9 and Bcl-2 were analyzed by Western blot (A) and Gel-Pro Analyzer 4.0 software (B) . ** p

    Article Snippet: Antibodies against caspase-3/-8/-9 were obtained from Cell Signaling Technology (Beverly, MA, USA).

    Techniques: Expressing, Western Blot, Software

    CDK1 mediates paclitaxel-induced chemoresistance through caspase-9 ( A ) Total caspase-9 and phospho-caspase-9 levels were analyzed by western blot. Actin was used as a loading control. CS, chemosenstive; CR, chemoresistant. ( B ) HeyA8-MDR and SKOV3-TR cells were treated with nocodazole to arrest cells in their mitosis. Noco, nocodazole; Als, alsterpaullone; PTX, paclitaxel. ( C ) Cell death was compared between three experimental groups: alsterpaullone-pretreated cells (upper left), alsterpaullonepretreated cells with added nocodazole (upper right), and alsterpaullone-pretreated cells with added paclitaxel and nocodazole (lower left). Administration of paclitaxel was followed by nocodazole treatment. ( D ) Populations of adherent (blue) and floating (green) cells are shown. Dead cells were stained with red dye.

    Journal: Oncotarget

    Article Title: Phosphorylation of caspase-9 at Thr125 directs paclitaxel resistance in ovarian cancer

    doi: 10.18632/oncotarget.23133

    Figure Lengend Snippet: CDK1 mediates paclitaxel-induced chemoresistance through caspase-9 ( A ) Total caspase-9 and phospho-caspase-9 levels were analyzed by western blot. Actin was used as a loading control. CS, chemosenstive; CR, chemoresistant. ( B ) HeyA8-MDR and SKOV3-TR cells were treated with nocodazole to arrest cells in their mitosis. Noco, nocodazole; Als, alsterpaullone; PTX, paclitaxel. ( C ) Cell death was compared between three experimental groups: alsterpaullone-pretreated cells (upper left), alsterpaullonepretreated cells with added nocodazole (upper right), and alsterpaullone-pretreated cells with added paclitaxel and nocodazole (lower left). Administration of paclitaxel was followed by nocodazole treatment. ( D ) Populations of adherent (blue) and floating (green) cells are shown. Dead cells were stained with red dye.

    Article Snippet: The membranes were incubated with antibodies against caspase-9 (BD Biosciences), phospho-caspase-9/Thr125 (Abcam), CDK1 and β-actin (Santa Cruz Biotechnology).

    Techniques: Western Blot, Staining

    Correlation between caspase-9 phosphorylation level and resistance to cell death ( A ) The colonies with different phosphorylation level were selected after low-dose paclitaxel treatment and cultured for 7 additional days. The ID number was conferred based on the phosphorylation level detected with western blot analysis. Relative density of phospho-caspase-9 was analyzed by Image J 3.0. ( B ) Different cells were treated with cisplatin for 12 h and cell viability assessed using MTT assay. ( C ) Cells were treated with paclitaxel and cell death assessed using MTT assay.

    Journal: Oncotarget

    Article Title: Phosphorylation of caspase-9 at Thr125 directs paclitaxel resistance in ovarian cancer

    doi: 10.18632/oncotarget.23133

    Figure Lengend Snippet: Correlation between caspase-9 phosphorylation level and resistance to cell death ( A ) The colonies with different phosphorylation level were selected after low-dose paclitaxel treatment and cultured for 7 additional days. The ID number was conferred based on the phosphorylation level detected with western blot analysis. Relative density of phospho-caspase-9 was analyzed by Image J 3.0. ( B ) Different cells were treated with cisplatin for 12 h and cell viability assessed using MTT assay. ( C ) Cells were treated with paclitaxel and cell death assessed using MTT assay.

    Article Snippet: The membranes were incubated with antibodies against caspase-9 (BD Biosciences), phospho-caspase-9/Thr125 (Abcam), CDK1 and β-actin (Santa Cruz Biotechnology).

    Techniques: Cell Culture, Western Blot, MTT Assay

    Caspase-9-dependent cell death ( A – B ) siRNA against caspase-9 was administrated in chemoresistant paired cell lines, HeyA8 and SKOV3. A mixture of random siRNAs was used as a control. ( C – D ) Cells were transfected with T125Amutant caspase-9, followed by treatment with alsterpaullone and paclitaxel. * , p

    Journal: Oncotarget

    Article Title: Phosphorylation of caspase-9 at Thr125 directs paclitaxel resistance in ovarian cancer

    doi: 10.18632/oncotarget.23133

    Figure Lengend Snippet: Caspase-9-dependent cell death ( A – B ) siRNA against caspase-9 was administrated in chemoresistant paired cell lines, HeyA8 and SKOV3. A mixture of random siRNAs was used as a control. ( C – D ) Cells were transfected with T125Amutant caspase-9, followed by treatment with alsterpaullone and paclitaxel. * , p

    Article Snippet: The membranes were incubated with antibodies against caspase-9 (BD Biosciences), phospho-caspase-9/Thr125 (Abcam), CDK1 and β-actin (Santa Cruz Biotechnology).

    Techniques: Transfection

    Schematic diagram explaining the mode of action of alsterpaullone Phosphorylation of caspase-9 indicates its inactive form. In the lower panel, the light color of CDK1 is intended to express the relatively reduced activity of CDK1.

    Journal: Oncotarget

    Article Title: Phosphorylation of caspase-9 at Thr125 directs paclitaxel resistance in ovarian cancer

    doi: 10.18632/oncotarget.23133

    Figure Lengend Snippet: Schematic diagram explaining the mode of action of alsterpaullone Phosphorylation of caspase-9 indicates its inactive form. In the lower panel, the light color of CDK1 is intended to express the relatively reduced activity of CDK1.

    Article Snippet: The membranes were incubated with antibodies against caspase-9 (BD Biosciences), phospho-caspase-9/Thr125 (Abcam), CDK1 and β-actin (Santa Cruz Biotechnology).

    Techniques: Activity Assay

    tRNA block apoptosome formation and caspase-9 activation

    Journal: Molecular cell

    Article Title: tRNA binds to cytochrome c and inhibits caspase activation

    doi: 10.1016/j.molcel.2010.01.023

    Figure Lengend Snippet: tRNA block apoptosome formation and caspase-9 activation

    Article Snippet: The following reagents were obtained from the indicated sources: bovine cytochrome c , yeast rRNA and tRNA, doxorubicin, proteinase K, formaldehyde, RNase A, Empigen BB, zVAD-FMK, and anti-Flag M2 beads (Sigma); protein A agarose and Texas Red-labeled dextran (Invitrogen); RNase Inhibitor (Promega); antibodies against caspase-9 (MBL International Corporation), caspase-3 (Santa Cruz Biotechnology), Smac (Cell Signaling), and actin (Sigma).

    Techniques: Blocking Assay, Activation Assay

    Interaction of cytochrome c with tRNA both in vivo and in vitro and the effect of tRNA on caspase-9 activation

    Journal: Molecular cell

    Article Title: tRNA binds to cytochrome c and inhibits caspase activation

    doi: 10.1016/j.molcel.2010.01.023

    Figure Lengend Snippet: Interaction of cytochrome c with tRNA both in vivo and in vitro and the effect of tRNA on caspase-9 activation

    Article Snippet: The following reagents were obtained from the indicated sources: bovine cytochrome c , yeast rRNA and tRNA, doxorubicin, proteinase K, formaldehyde, RNase A, Empigen BB, zVAD-FMK, and anti-Flag M2 beads (Sigma); protein A agarose and Texas Red-labeled dextran (Invitrogen); RNase Inhibitor (Promega); antibodies against caspase-9 (MBL International Corporation), caspase-3 (Santa Cruz Biotechnology), Smac (Cell Signaling), and actin (Sigma).

    Techniques: In Vivo, In Vitro, Activation Assay

    Effects of putative MCL-1 inhibitors on Bax/Bak- and caspase-9-deficient MEFs. MEFs deficient in either Bax and Bak (dotted lines) or caspase-9 (dashed lines) along with their wild-type counterparts (continuous bold lines) were exposed for 24 h

    Journal: Cell Death and Differentiation

    Article Title: Evaluation and critical assessment of putative MCL-1 inhibitors

    doi: 10.1038/cdd.2013.79

    Figure Lengend Snippet: Effects of putative MCL-1 inhibitors on Bax/Bak- and caspase-9-deficient MEFs. MEFs deficient in either Bax and Bak (dotted lines) or caspase-9 (dashed lines) along with their wild-type counterparts (continuous bold lines) were exposed for 24 h

    Article Snippet: MIM-1 was synthesised as previously described.21 Antibody against murine caspase-9 was obtained from Abcam (Cambridge, UK) and antibodies against NOXA and tubulin from Calbiochem, MCL-1 from Santa Cruz Biotechnology (Santa Cruz, CA, USA), BAX and BAK from Millipore (Watford, UK), PARP from Enzo Life Sciences (Exeter, UK) and human caspase-9 antiserum (a gift from Dr. X.

    Techniques: