anti-tgfα gf10 Search Results


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  • 86
    Thermo Fisher anti phosphorylated egfr tyr1173
    Anti Phosphorylated Egfr Tyr1173, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 86 stars, based on 1 article reviews
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    93
    Millipore tgf α
    (A) Parathyroid AP2 expression correlates directly with PCNA in human SH. (Top) Western blot analysis of AP2, PCNA, and GAPDH content in whole-cell extracts from 10 human parathyroid glands with no pathologic diagnosis of diffuse or nodular hyperplasia. (Bottom) Correlation between AP2 and PCNA content, as measured by densitometric analysis of AP2/GAPDH and PCNA/GAPDH ratios in whole-cell extracts from 10 hyperplastic human parathyroid glands depicted in the Western blot. (B) Parathyroid AP2 expression correlates directly with <t>TGF-α</t> content in human SH. Representative photomicrographs of immunofluorescence staining for AP2 and TGF-α in diffuse and nodular human parathyroid glands. Magnification, ×200.
    Tgf α, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 203 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Santa Cruz Biotechnology rabbit anti egfr
    Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and <t>EGFR,</t> <t>pEGFR,</t> VEGFR2, or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for VEGFR1 and CD31 (endothelial cells) and F4/80 (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.
    Rabbit Anti Egfr, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 303 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Santa Cruz Biotechnology rabbit anti vegfr2
    Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and EGFR, pEGFR, <t>VEGFR2,</t> or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for VEGFR1 and CD31 (endothelial cells) and F4/80 (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.
    Rabbit Anti Vegfr2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Agilent technologies hrp
    Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and EGFR, pEGFR, <t>VEGFR2,</t> or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for VEGFR1 and CD31 (endothelial cells) and F4/80 (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.
    Hrp, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 1698 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Cell Signaling Technology Inc anti phosphorylated egfr tyrl068
    Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and EGFR, pEGFR, <t>VEGFR2,</t> or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for VEGFR1 and CD31 (endothelial cells) and F4/80 (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.
    Anti Phosphorylated Egfr Tyrl068, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson anti cd31 mek13 3
    Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and EGFR, pEGFR, <t>VEGFR2,</t> or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for VEGFR1 and CD31 (endothelial cells) and F4/80 (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.
    Anti Cd31 Mek13 3, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology anti vascular endothelial growth factor vegf a
    Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and EGFR, pEGFR, <t>VEGFR2,</t> or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for VEGFR1 and CD31 (endothelial cells) and F4/80 (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.
    Anti Vascular Endothelial Growth Factor Vegf A, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Trinity Biotech anti lymphatic vessel endothelial hyaluronic acid receptor lyve1 103pa50ag and 10350pa50s
    Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and EGFR, pEGFR, <t>VEGFR2,</t> or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for VEGFR1 and CD31 (endothelial cells) and F4/80 (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.
    Anti Lymphatic Vessel Endothelial Hyaluronic Acid Receptor Lyve1 103pa50ag And 10350pa50s, supplied by Trinity Biotech, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Santa Cruz Biotechnology anti egfr
    Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and EGFR, pEGFR, <t>VEGFR2,</t> or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for VEGFR1 and CD31 (endothelial cells) and F4/80 (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.
    Anti Egfr, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1518 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology anti vegfc sc9047
    Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and EGFR, pEGFR, <t>VEGFR2,</t> or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for VEGFR1 and CD31 (endothelial cells) and F4/80 (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.
    Anti Vegfc Sc9047, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies anti α smooth muscle actin α sma ah1
    Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and EGFR, pEGFR, <t>VEGFR2,</t> or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for VEGFR1 and CD31 (endothelial cells) and F4/80 (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.
    Anti α Smooth Muscle Actin α Sma Ah1, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Bio-Rad anti f4 80 mcap497
    Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and EGFR, pEGFR, <t>VEGFR2,</t> or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for VEGFR1 and CD31 (endothelial cells) and F4/80 (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.
    Anti F4 80 Mcap497, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore anti matrix metalloproteinase mmp 2
    Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and EGFR, pEGFR, <t>VEGFR2,</t> or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for VEGFR1 and CD31 (endothelial cells) and F4/80 (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.
    Anti Matrix Metalloproteinase Mmp 2, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biocare Medical biotinylated goat anti rabbit igg
    Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and EGFR, pEGFR, <t>VEGFR2,</t> or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for VEGFR1 and CD31 (endothelial cells) and F4/80 (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.
    Biotinylated Goat Anti Rabbit Igg, supplied by Biocare Medical, used in various techniques. Bioz Stars score: 93/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno chrompure rabbit igg
    Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and EGFR, pEGFR, <t>VEGFR2,</t> or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for VEGFR1 and CD31 (endothelial cells) and F4/80 (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.
    Chrompure Rabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 98/100, based on 563 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno chrompure mouse igg
    Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and EGFR, pEGFR, <t>VEGFR2,</t> or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for VEGFR1 and CD31 (endothelial cells) and F4/80 (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.
    Chrompure Mouse Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 97/100, based on 423 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Jackson Immuno chrompure rat igg
    Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and EGFR, pEGFR, <t>VEGFR2,</t> or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for VEGFR1 and CD31 (endothelial cells) and F4/80 (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.
    Chrompure Rat Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 95/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher cxcl8 polyclonal antibody
    Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and EGFR, pEGFR, <t>VEGFR2,</t> or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for VEGFR1 and CD31 (endothelial cells) and F4/80 (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.
    Cxcl8 Polyclonal Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam human ptg
    Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and EGFR, pEGFR, <t>VEGFR2,</t> or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for VEGFR1 and CD31 (endothelial cells) and F4/80 (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.
    Human Ptg, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Cell Signaling Technology Inc rabbit anti pegfr
    Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and <t>EGFR,</t> <t>pEGFR,</t> VEGFR2, or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for VEGFR1 and CD31 (endothelial cells) and F4/80 (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.
    Rabbit Anti Pegfr, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 88/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology rabbit anti vegfr1
    Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and EGFR, pEGFR, VEGFR2, or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for <t>VEGFR1</t> and CD31 (endothelial cells) and F4/80 (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.
    Rabbit Anti Vegfr1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 88/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Bio-Rad rabbit anti mouse f4 80
    Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and EGFR, pEGFR, VEGFR2, or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for VEGFR1 and CD31 (endothelial cells) and <t>F4/80</t> (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.
    Rabbit Anti Mouse F4 80, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 84/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Agilent technologies citrate buffer
    Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and EGFR, pEGFR, VEGFR2, or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for VEGFR1 and CD31 (endothelial cells) and <t>F4/80</t> (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.
    Citrate Buffer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 7834 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore streptavidin biotin method
    Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and EGFR, pEGFR, VEGFR2, or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for VEGFR1 and CD31 (endothelial cells) and <t>F4/80</t> (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.
    Streptavidin Biotin Method, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 5 article reviews
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    streptavidin biotin method - by Bioz Stars, 2020-09
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    Image Search Results


    (A) Parathyroid AP2 expression correlates directly with PCNA in human SH. (Top) Western blot analysis of AP2, PCNA, and GAPDH content in whole-cell extracts from 10 human parathyroid glands with no pathologic diagnosis of diffuse or nodular hyperplasia. (Bottom) Correlation between AP2 and PCNA content, as measured by densitometric analysis of AP2/GAPDH and PCNA/GAPDH ratios in whole-cell extracts from 10 hyperplastic human parathyroid glands depicted in the Western blot. (B) Parathyroid AP2 expression correlates directly with TGF-α content in human SH. Representative photomicrographs of immunofluorescence staining for AP2 and TGF-α in diffuse and nodular human parathyroid glands. Magnification, ×200.

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: Activator Protein 2? Mediates Parathyroid TGF-? Self-Induction in Secondary Hyperparathyroidism

    doi: 10.1681/ASN.2007111216

    Figure Lengend Snippet: (A) Parathyroid AP2 expression correlates directly with PCNA in human SH. (Top) Western blot analysis of AP2, PCNA, and GAPDH content in whole-cell extracts from 10 human parathyroid glands with no pathologic diagnosis of diffuse or nodular hyperplasia. (Bottom) Correlation between AP2 and PCNA content, as measured by densitometric analysis of AP2/GAPDH and PCNA/GAPDH ratios in whole-cell extracts from 10 hyperplastic human parathyroid glands depicted in the Western blot. (B) Parathyroid AP2 expression correlates directly with TGF-α content in human SH. Representative photomicrographs of immunofluorescence staining for AP2 and TGF-α in diffuse and nodular human parathyroid glands. Magnification, ×200.

    Article Snippet: Sections from human parathyroid glands were incubated overnight at 4°C simultaneously with primary antibodies against AP2 (SC184, 1:1000 dilution; Santa Cruz Biotechnology) and TGF-α (GF10, 1:20 dilution; Calbiochem, San Diego, CA).

    Techniques: Expressing, Western Blot, Immunofluorescence, Staining

    AP2 contribution to TGF-α self-induction in A431 cells. (A) Erlotinib dosage-dependent inhibition of TGF-α/EGFR–driven growth as measured by progressive reduction in cell number (top) and MTT assay (bottom). Bars and error bars indicate means ± SEM of erlotinib-induced reduction in viable cells compared with untreated controls (untreated, erlotinib 0 μM) from four independent experiments. (B) Western blot analysis of changes in AP2 expression in response to increasing dosages of erlotinib in A431 cells treated as in A. (C) Quantification of changes in nuclear AP2 expression using immunofluorescent staining for AP2, in response to erlotinib. Bars and error bars represent means ± SEM of the number of AP2-positive nuclei per 1000 cells from three independent experiments. Results are expressed as percentage of nuclear AP2 content relative to untreated (erlotinib 0 μM) cells. (D) Erlotinib dosage-dependent inhibition of TGF-α mRNA levels in cells treated as in A. (Top) Representative RT-PCR of TGF-α mRNA expression in response to erlotinib. (Bottom) Bars and error bars represent the means ± SEM of TGF-α mRNA levels, corrected per GAPDH, from two independent experiments. Results are expressed as percentage of untreated A431 cells (0). (E, top) Partial sequence of the human TGF-α promoter containing an AP2 binding site (wild-type) and the insertion of a 10-bp mutation at the core AP2 consensus (mutant). (E, middle) Promoter activity in response to TGF-α treatment in A431 cells transfected with wild-type (WT; □) or mutant (□) TGF-α promoter/luciferase reporter. Bars and error bars indicate means ± SEM from triplicate luciferase/β-gal determinations per experimental condition from two independent experiments ( P

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: Activator Protein 2? Mediates Parathyroid TGF-? Self-Induction in Secondary Hyperparathyroidism

    doi: 10.1681/ASN.2007111216

    Figure Lengend Snippet: AP2 contribution to TGF-α self-induction in A431 cells. (A) Erlotinib dosage-dependent inhibition of TGF-α/EGFR–driven growth as measured by progressive reduction in cell number (top) and MTT assay (bottom). Bars and error bars indicate means ± SEM of erlotinib-induced reduction in viable cells compared with untreated controls (untreated, erlotinib 0 μM) from four independent experiments. (B) Western blot analysis of changes in AP2 expression in response to increasing dosages of erlotinib in A431 cells treated as in A. (C) Quantification of changes in nuclear AP2 expression using immunofluorescent staining for AP2, in response to erlotinib. Bars and error bars represent means ± SEM of the number of AP2-positive nuclei per 1000 cells from three independent experiments. Results are expressed as percentage of nuclear AP2 content relative to untreated (erlotinib 0 μM) cells. (D) Erlotinib dosage-dependent inhibition of TGF-α mRNA levels in cells treated as in A. (Top) Representative RT-PCR of TGF-α mRNA expression in response to erlotinib. (Bottom) Bars and error bars represent the means ± SEM of TGF-α mRNA levels, corrected per GAPDH, from two independent experiments. Results are expressed as percentage of untreated A431 cells (0). (E, top) Partial sequence of the human TGF-α promoter containing an AP2 binding site (wild-type) and the insertion of a 10-bp mutation at the core AP2 consensus (mutant). (E, middle) Promoter activity in response to TGF-α treatment in A431 cells transfected with wild-type (WT; □) or mutant (□) TGF-α promoter/luciferase reporter. Bars and error bars indicate means ± SEM from triplicate luciferase/β-gal determinations per experimental condition from two independent experiments ( P

    Article Snippet: Sections from human parathyroid glands were incubated overnight at 4°C simultaneously with primary antibodies against AP2 (SC184, 1:1000 dilution; Santa Cruz Biotechnology) and TGF-α (GF10, 1:20 dilution; Calbiochem, San Diego, CA).

    Techniques: Inhibition, MTT Assay, Western Blot, Expressing, Staining, Reverse Transcription Polymerase Chain Reaction, Sequencing, Binding Assay, Mutagenesis, Activity Assay, Transfection, Luciferase

    Model for AP2 regulation of TGF-α self-induction. The release of the mature form of TGF-α from its cell membrane precursor binds and activates its receptor, the EGFR, thereby inducing increases in AP2 expression, nuclear translocation, and transactivation of the TGF-α gene. Inhibition of EGFR activation with erlotinib is sufficient to simultaneously arrest increases in AP2 and AP2-mediated TGF-α gene transcription.

    Journal: Journal of the American Society of Nephrology : JASN

    Article Title: Activator Protein 2? Mediates Parathyroid TGF-? Self-Induction in Secondary Hyperparathyroidism

    doi: 10.1681/ASN.2007111216

    Figure Lengend Snippet: Model for AP2 regulation of TGF-α self-induction. The release of the mature form of TGF-α from its cell membrane precursor binds and activates its receptor, the EGFR, thereby inducing increases in AP2 expression, nuclear translocation, and transactivation of the TGF-α gene. Inhibition of EGFR activation with erlotinib is sufficient to simultaneously arrest increases in AP2 and AP2-mediated TGF-α gene transcription.

    Article Snippet: Sections from human parathyroid glands were incubated overnight at 4°C simultaneously with primary antibodies against AP2 (SC184, 1:1000 dilution; Santa Cruz Biotechnology) and TGF-α (GF10, 1:20 dilution; Calbiochem, San Diego, CA).

    Techniques: Expressing, Translocation Assay, Inhibition, Activation Assay

    Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and EGFR, pEGFR, VEGFR2, or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for VEGFR1 and CD31 (endothelial cells) and F4/80 (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.

    Journal: Neoplasia (New York, N.Y.)

    Article Title: Inhibition of Epidermal Growth Factor Receptor and Vascular Endothelial Growth Factor Receptor Phosphorylation on Tumor-Associated Endothelial Cells Leads to Treatment of Orthotopic Human Colon Cancer in Nude Mice 1

    doi:

    Figure Lengend Snippet: Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and EGFR, pEGFR, VEGFR2, or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for VEGFR1 and CD31 (endothelial cells) and F4/80 (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.

    Article Snippet: The following primary antibodies were purchased: rabbit anti-EGFR (SC03; Santa Cruz Biotechnology, Santa Cruz, CA); rabbit anti-phosphorylated EGFR (pEGFR) (Tyr1173; Biosource, Camarillo, CA); rabbit anti-VEGFR2 (Flk-1, C1158; Santa Cruz Biotechnology); rabbit anti-phosphorylated VEGFR2 (pVEGFR2) (Flk-1, PC460; Oncogene, Boston, MA); rabbit anti-VEGFR1 (C17; Santa Cruz Biotechnology); rabbit anti-mouse F4/80 (MCAP497; Serotec, Raleigh, NC) and rat anti-mouse CD31 (BD PharMingen, San Diego, CA) for frozen samples; mouse anti-human TGF-α (GF10; Oncogene); mouse anti-human VEGF (23071D; BD Phar-Mingen) and Ki-67 (MIB-1; DakoCytomation, Carpinteria, CA) for paraffin-embedded samples; and rabbit anti-EGFR (SC03; Santa Cruz Biotechnology), rabbit anti-pEGFR (Tyr1068; Cell Signaling Technology, Danvers, MA), and rabbit anti-VEGFR2 (Flk-1, C1158; Santa Cruz Biotechnology) for Western blot analysis.

    Techniques: Immunohistochemistry, Mouse Assay, Double Immunofluorescence Staining, Expressing

    Double immunofluorescence staining for expression of EGFR, pEGFR, VEGFR, or pVEGFR in tumor-associated endothelial cells. (A) Tissue sections were stained with anti -CD31/PECAM1 antibody (red) and with anti-EGFR, pEGFR, VEGFR, or pVEGFR (green) as described in the Materials and Methods section. Colocalization of CD31 and EGFR, pEGFR, VEGFR, or pVEGFR appears as yellow fluorescence. Expression of EGFR and VEGFR by tumor-associated endothelial cells was found in tumors from all treatment groups. Phosphorylation of EGFR and VEGFR on endothelial cells was inhibited by treatment with AEE788 or AEE788 plus CPT-11. Note that treatment with AEE788 alone decreased the number and diameter of blood vessels and that this effect was even more pronounced with combined AEE788 and CPT-11.

    Journal: Neoplasia (New York, N.Y.)

    Article Title: Inhibition of Epidermal Growth Factor Receptor and Vascular Endothelial Growth Factor Receptor Phosphorylation on Tumor-Associated Endothelial Cells Leads to Treatment of Orthotopic Human Colon Cancer in Nude Mice 1

    doi:

    Figure Lengend Snippet: Double immunofluorescence staining for expression of EGFR, pEGFR, VEGFR, or pVEGFR in tumor-associated endothelial cells. (A) Tissue sections were stained with anti -CD31/PECAM1 antibody (red) and with anti-EGFR, pEGFR, VEGFR, or pVEGFR (green) as described in the Materials and Methods section. Colocalization of CD31 and EGFR, pEGFR, VEGFR, or pVEGFR appears as yellow fluorescence. Expression of EGFR and VEGFR by tumor-associated endothelial cells was found in tumors from all treatment groups. Phosphorylation of EGFR and VEGFR on endothelial cells was inhibited by treatment with AEE788 or AEE788 plus CPT-11. Note that treatment with AEE788 alone decreased the number and diameter of blood vessels and that this effect was even more pronounced with combined AEE788 and CPT-11.

    Article Snippet: The following primary antibodies were purchased: rabbit anti-EGFR (SC03; Santa Cruz Biotechnology, Santa Cruz, CA); rabbit anti-phosphorylated EGFR (pEGFR) (Tyr1173; Biosource, Camarillo, CA); rabbit anti-VEGFR2 (Flk-1, C1158; Santa Cruz Biotechnology); rabbit anti-phosphorylated VEGFR2 (pVEGFR2) (Flk-1, PC460; Oncogene, Boston, MA); rabbit anti-VEGFR1 (C17; Santa Cruz Biotechnology); rabbit anti-mouse F4/80 (MCAP497; Serotec, Raleigh, NC) and rat anti-mouse CD31 (BD PharMingen, San Diego, CA) for frozen samples; mouse anti-human TGF-α (GF10; Oncogene); mouse anti-human VEGF (23071D; BD Phar-Mingen) and Ki-67 (MIB-1; DakoCytomation, Carpinteria, CA) for paraffin-embedded samples; and rabbit anti-EGFR (SC03; Santa Cruz Biotechnology), rabbit anti-pEGFR (Tyr1068; Cell Signaling Technology, Danvers, MA), and rabbit anti-VEGFR2 (Flk-1, C1158; Santa Cruz Biotechnology) for Western blot analysis.

    Techniques: Double Immunofluorescence Staining, Expressing, Staining, Fluorescence, Cycling Probe Technology

    Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and EGFR, pEGFR, VEGFR2, or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for VEGFR1 and CD31 (endothelial cells) and F4/80 (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.

    Journal: Neoplasia (New York, N.Y.)

    Article Title: Inhibition of Epidermal Growth Factor Receptor and Vascular Endothelial Growth Factor Receptor Phosphorylation on Tumor-Associated Endothelial Cells Leads to Treatment of Orthotopic Human Colon Cancer in Nude Mice 1

    doi:

    Figure Lengend Snippet: Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and EGFR, pEGFR, VEGFR2, or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for VEGFR1 and CD31 (endothelial cells) and F4/80 (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.

    Article Snippet: The following primary antibodies were purchased: rabbit anti-EGFR (SC03; Santa Cruz Biotechnology, Santa Cruz, CA); rabbit anti-phosphorylated EGFR (pEGFR) (Tyr1173; Biosource, Camarillo, CA); rabbit anti-VEGFR2 (Flk-1, C1158; Santa Cruz Biotechnology); rabbit anti-phosphorylated VEGFR2 (pVEGFR2) (Flk-1, PC460; Oncogene, Boston, MA); rabbit anti-VEGFR1 (C17; Santa Cruz Biotechnology); rabbit anti-mouse F4/80 (MCAP497; Serotec, Raleigh, NC) and rat anti-mouse CD31 (BD PharMingen, San Diego, CA) for frozen samples; mouse anti-human TGF-α (GF10; Oncogene); mouse anti-human VEGF (23071D; BD Phar-Mingen) and Ki-67 (MIB-1; DakoCytomation, Carpinteria, CA) for paraffin-embedded samples; and rabbit anti-EGFR (SC03; Santa Cruz Biotechnology), rabbit anti-pEGFR (Tyr1068; Cell Signaling Technology, Danvers, MA), and rabbit anti-VEGFR2 (Flk-1, C1158; Santa Cruz Biotechnology) for Western blot analysis.

    Techniques: Immunohistochemistry, Mouse Assay, Double Immunofluorescence Staining, Expressing

    Expression of TGF-α, EGFR, VEGFR2, and HER2 by SW620CE2 cells growing in culture. Western blot and RT-PCR analyses reveal that the SW620CE2 cells do not express (A) EGFR, (B) HER2, or (C) VEGFR2 protein. KM12C human colon cancer cells served as a positive control for EGFR expression, and SKOV3ip1 human ovarian cancer cells served as a positive control for VEGFR2 and HER2 expression. The SW620CE2 cells produced high levels of (D) TGF-α but not (E) EGFR or VEGFR2 mRNA.

    Journal: Neoplasia (New York, N.Y.)

    Article Title: Inhibition of Epidermal Growth Factor Receptor and Vascular Endothelial Growth Factor Receptor Phosphorylation on Tumor-Associated Endothelial Cells Leads to Treatment of Orthotopic Human Colon Cancer in Nude Mice 1

    doi:

    Figure Lengend Snippet: Expression of TGF-α, EGFR, VEGFR2, and HER2 by SW620CE2 cells growing in culture. Western blot and RT-PCR analyses reveal that the SW620CE2 cells do not express (A) EGFR, (B) HER2, or (C) VEGFR2 protein. KM12C human colon cancer cells served as a positive control for EGFR expression, and SKOV3ip1 human ovarian cancer cells served as a positive control for VEGFR2 and HER2 expression. The SW620CE2 cells produced high levels of (D) TGF-α but not (E) EGFR or VEGFR2 mRNA.

    Article Snippet: The following primary antibodies were purchased: rabbit anti-EGFR (SC03; Santa Cruz Biotechnology, Santa Cruz, CA); rabbit anti-phosphorylated EGFR (pEGFR) (Tyr1173; Biosource, Camarillo, CA); rabbit anti-VEGFR2 (Flk-1, C1158; Santa Cruz Biotechnology); rabbit anti-phosphorylated VEGFR2 (pVEGFR2) (Flk-1, PC460; Oncogene, Boston, MA); rabbit anti-VEGFR1 (C17; Santa Cruz Biotechnology); rabbit anti-mouse F4/80 (MCAP497; Serotec, Raleigh, NC) and rat anti-mouse CD31 (BD PharMingen, San Diego, CA) for frozen samples; mouse anti-human TGF-α (GF10; Oncogene); mouse anti-human VEGF (23071D; BD Phar-Mingen) and Ki-67 (MIB-1; DakoCytomation, Carpinteria, CA) for paraffin-embedded samples; and rabbit anti-EGFR (SC03; Santa Cruz Biotechnology), rabbit anti-pEGFR (Tyr1068; Cell Signaling Technology, Danvers, MA), and rabbit anti-VEGFR2 (Flk-1, C1158; Santa Cruz Biotechnology) for Western blot analysis.

    Techniques: Expressing, Western Blot, Reverse Transcription Polymerase Chain Reaction, Positive Control, Produced

    Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and EGFR, pEGFR, VEGFR2, or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for VEGFR1 and CD31 (endothelial cells) and F4/80 (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.

    Journal: Neoplasia (New York, N.Y.)

    Article Title: Inhibition of Epidermal Growth Factor Receptor and Vascular Endothelial Growth Factor Receptor Phosphorylation on Tumor-Associated Endothelial Cells Leads to Treatment of Orthotopic Human Colon Cancer in Nude Mice 1

    doi:

    Figure Lengend Snippet: Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and EGFR, pEGFR, VEGFR2, or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for VEGFR1 and CD31 (endothelial cells) and F4/80 (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.

    Article Snippet: The following primary antibodies were purchased: rabbit anti-EGFR (SC03; Santa Cruz Biotechnology, Santa Cruz, CA); rabbit anti-phosphorylated EGFR (pEGFR) (Tyr1173; Biosource, Camarillo, CA); rabbit anti-VEGFR2 (Flk-1, C1158; Santa Cruz Biotechnology); rabbit anti-phosphorylated VEGFR2 (pVEGFR2) (Flk-1, PC460; Oncogene, Boston, MA); rabbit anti-VEGFR1 (C17; Santa Cruz Biotechnology); rabbit anti-mouse F4/80 (MCAP497; Serotec, Raleigh, NC) and rat anti-mouse CD31 (BD PharMingen, San Diego, CA) for frozen samples; mouse anti-human TGF-α (GF10; Oncogene); mouse anti-human VEGF (23071D; BD Phar-Mingen) and Ki-67 (MIB-1; DakoCytomation, Carpinteria, CA) for paraffin-embedded samples; and rabbit anti-EGFR (SC03; Santa Cruz Biotechnology), rabbit anti-pEGFR (Tyr1068; Cell Signaling Technology, Danvers, MA), and rabbit anti-VEGFR2 (Flk-1, C1158; Santa Cruz Biotechnology) for Western blot analysis.

    Techniques: Immunohistochemistry, Mouse Assay, Double Immunofluorescence Staining, Expressing

    Double immunofluorescence staining for expression of EGFR, pEGFR, VEGFR, or pVEGFR in tumor-associated endothelial cells. (A) Tissue sections were stained with anti -CD31/PECAM1 antibody (red) and with anti-EGFR, pEGFR, VEGFR, or pVEGFR (green) as described in the Materials and Methods section. Colocalization of CD31 and EGFR, pEGFR, VEGFR, or pVEGFR appears as yellow fluorescence. Expression of EGFR and VEGFR by tumor-associated endothelial cells was found in tumors from all treatment groups. Phosphorylation of EGFR and VEGFR on endothelial cells was inhibited by treatment with AEE788 or AEE788 plus CPT-11. Note that treatment with AEE788 alone decreased the number and diameter of blood vessels and that this effect was even more pronounced with combined AEE788 and CPT-11.

    Journal: Neoplasia (New York, N.Y.)

    Article Title: Inhibition of Epidermal Growth Factor Receptor and Vascular Endothelial Growth Factor Receptor Phosphorylation on Tumor-Associated Endothelial Cells Leads to Treatment of Orthotopic Human Colon Cancer in Nude Mice 1

    doi:

    Figure Lengend Snippet: Double immunofluorescence staining for expression of EGFR, pEGFR, VEGFR, or pVEGFR in tumor-associated endothelial cells. (A) Tissue sections were stained with anti -CD31/PECAM1 antibody (red) and with anti-EGFR, pEGFR, VEGFR, or pVEGFR (green) as described in the Materials and Methods section. Colocalization of CD31 and EGFR, pEGFR, VEGFR, or pVEGFR appears as yellow fluorescence. Expression of EGFR and VEGFR by tumor-associated endothelial cells was found in tumors from all treatment groups. Phosphorylation of EGFR and VEGFR on endothelial cells was inhibited by treatment with AEE788 or AEE788 plus CPT-11. Note that treatment with AEE788 alone decreased the number and diameter of blood vessels and that this effect was even more pronounced with combined AEE788 and CPT-11.

    Article Snippet: The following primary antibodies were purchased: rabbit anti-EGFR (SC03; Santa Cruz Biotechnology, Santa Cruz, CA); rabbit anti-phosphorylated EGFR (pEGFR) (Tyr1173; Biosource, Camarillo, CA); rabbit anti-VEGFR2 (Flk-1, C1158; Santa Cruz Biotechnology); rabbit anti-phosphorylated VEGFR2 (pVEGFR2) (Flk-1, PC460; Oncogene, Boston, MA); rabbit anti-VEGFR1 (C17; Santa Cruz Biotechnology); rabbit anti-mouse F4/80 (MCAP497; Serotec, Raleigh, NC) and rat anti-mouse CD31 (BD PharMingen, San Diego, CA) for frozen samples; mouse anti-human TGF-α (GF10; Oncogene); mouse anti-human VEGF (23071D; BD Phar-Mingen) and Ki-67 (MIB-1; DakoCytomation, Carpinteria, CA) for paraffin-embedded samples; and rabbit anti-EGFR (SC03; Santa Cruz Biotechnology), rabbit anti-pEGFR (Tyr1068; Cell Signaling Technology, Danvers, MA), and rabbit anti-VEGFR2 (Flk-1, C1158; Santa Cruz Biotechnology) for Western blot analysis.

    Techniques: Double Immunofluorescence Staining, Expressing, Staining, Fluorescence, Cycling Probe Technology

    Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and EGFR, pEGFR, VEGFR2, or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for VEGFR1 and CD31 (endothelial cells) and F4/80 (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.

    Journal: Neoplasia (New York, N.Y.)

    Article Title: Inhibition of Epidermal Growth Factor Receptor and Vascular Endothelial Growth Factor Receptor Phosphorylation on Tumor-Associated Endothelial Cells Leads to Treatment of Orthotopic Human Colon Cancer in Nude Mice 1

    doi:

    Figure Lengend Snippet: Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and EGFR, pEGFR, VEGFR2, or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for VEGFR1 and CD31 (endothelial cells) and F4/80 (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.

    Article Snippet: The following primary antibodies were purchased: rabbit anti-EGFR (SC03; Santa Cruz Biotechnology, Santa Cruz, CA); rabbit anti-phosphorylated EGFR (pEGFR) (Tyr1173; Biosource, Camarillo, CA); rabbit anti-VEGFR2 (Flk-1, C1158; Santa Cruz Biotechnology); rabbit anti-phosphorylated VEGFR2 (pVEGFR2) (Flk-1, PC460; Oncogene, Boston, MA); rabbit anti-VEGFR1 (C17; Santa Cruz Biotechnology); rabbit anti-mouse F4/80 (MCAP497; Serotec, Raleigh, NC) and rat anti-mouse CD31 (BD PharMingen, San Diego, CA) for frozen samples; mouse anti-human TGF-α (GF10; Oncogene); mouse anti-human VEGF (23071D; BD Phar-Mingen) and Ki-67 (MIB-1; DakoCytomation, Carpinteria, CA) for paraffin-embedded samples; and rabbit anti-EGFR (SC03; Santa Cruz Biotechnology), rabbit anti-pEGFR (Tyr1068; Cell Signaling Technology, Danvers, MA), and rabbit anti-VEGFR2 (Flk-1, C1158; Santa Cruz Biotechnology) for Western blot analysis.

    Techniques: Immunohistochemistry, Mouse Assay, Double Immunofluorescence Staining, Expressing

    Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and EGFR, pEGFR, VEGFR2, or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for VEGFR1 and CD31 (endothelial cells) and F4/80 (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.

    Journal: Neoplasia (New York, N.Y.)

    Article Title: Inhibition of Epidermal Growth Factor Receptor and Vascular Endothelial Growth Factor Receptor Phosphorylation on Tumor-Associated Endothelial Cells Leads to Treatment of Orthotopic Human Colon Cancer in Nude Mice 1

    doi:

    Figure Lengend Snippet: Immunohistochemical analysis of SW620CE2 cells growing in the cecum of nude mice. Double immunofluorescence staining was used for CD31/PECAM-1 and EGFR, pEGFR, VEGFR2, or pVEGFR2. (A) The SW620CE2 cells expressed TGF-α and VEGF but not EGFR or VEGFR. The KM12C cells expressed TGF-α and EGFR (green), and the SKOV3ip1 cells (growing in the peritoneal cavity) expressed VEGF and VEGFR2 (green). In all tumors, the endothelial cells expressed EGFR and VEGFR2 (yellow). (B) Colocalization for VEGFR1 and CD31 (endothelial cells) and F4/80 (macrophages) in SW620CE2 cecal tumors. (C) Expression of HER2 (green) in SKOV3ipl but not SW620CE2 tumors.

    Article Snippet: The following primary antibodies were purchased: rabbit anti-EGFR (SC03; Santa Cruz Biotechnology, Santa Cruz, CA); rabbit anti-phosphorylated EGFR (pEGFR) (Tyr1173; Biosource, Camarillo, CA); rabbit anti-VEGFR2 (Flk-1, C1158; Santa Cruz Biotechnology); rabbit anti-phosphorylated VEGFR2 (pVEGFR2) (Flk-1, PC460; Oncogene, Boston, MA); rabbit anti-VEGFR1 (C17; Santa Cruz Biotechnology); rabbit anti-mouse F4/80 (MCAP497; Serotec, Raleigh, NC) and rat anti-mouse CD31 (BD PharMingen, San Diego, CA) for frozen samples; mouse anti-human TGF-α (GF10; Oncogene); mouse anti-human VEGF (23071D; BD Phar-Mingen) and Ki-67 (MIB-1; DakoCytomation, Carpinteria, CA) for paraffin-embedded samples; and rabbit anti-EGFR (SC03; Santa Cruz Biotechnology), rabbit anti-pEGFR (Tyr1068; Cell Signaling Technology, Danvers, MA), and rabbit anti-VEGFR2 (Flk-1, C1158; Santa Cruz Biotechnology) for Western blot analysis.

    Techniques: Immunohistochemistry, Mouse Assay, Double Immunofluorescence Staining, Expressing