Journal: Cancer Management and Research
Article Title: Uridine-cytidine kinase 2 promotes metastasis of hepatocellular carcinoma cells via the Stat3 pathway
Figure Lengend Snippet: Uck2 regulated the expression of MMPs and EMT progression via the Stat3 signaling pathway. Notes: ( A ) Raw photos and densitometry analysis of Western blotting analysis: treatment with the Stat3 inhibitor WP1066 neutralized the upregulation of MMP2 and MMP9 and reversed the EMT progression caused by the overexpression of Uck2 in Bel-7402 cells. ( B and C ) After treatment with a Stat3 inhibitor (3 µM WP1066) for 24 hours, Bel-7402/Uck2 cells showed decreased migration ability and decreased invasion ability by a transwell assay. β-actin was used as the housekeeping gene to normalize expression levels in the densitometry analysis by ImageJ software (1.48 version; NIH, Bethesda, MD, USA). The experiments were repeated three times, and the results are shown as the mean ± SD; * P
Article Snippet: The following anti-Homo sapiens primary antibodies were used for Western blotting: mouse anti-β-actin (1:5,000 dilution, Proteintech, 60008-1-Ig); rabbit anti-Uck2 (1:1,000 dilution, Proteintech, 10511-1-AP); rabbit anti-MMP2 (1:1,000 dilution, ImmunoWay, YT2798); rabbit anti-MMP7 (1:1,000 dilution, Immuno-Way, YT2663); rabbit anti-MMP9 (1:1,000 dilution, Immu-noWay, YT1892); rabbit anti-E-cadherin (1:500 dilution, Abcam, ab15148); rabbit anti-N-cadherin (1:1,000 dilution, Abcam, ab76057); mouse anti-Viomentin (1:1,000 dilution, Abcam, ab8978); rabbit anti-fibronectin (1:1,000 dilution, Abcam, ab32419); rabbit anti-p44/42 MAPK (Erk1/2) (1:2,000 dilution, CST, #4695); rabbit anti-phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (1:2,000 dilution, CST, #4370); mouse anti-Stat3 (1:2,000 dilution, CST, #9139); rabbit anti-phospho-Stat3 (Try705) (1:2,000 dilution, CST, #9145); mouse anti-Akt (1:2,000 dilution, CST, #2920); and rabbit anti-phospho-Akt (Ser473) (1:2,000 dilution, CST, #4060).
Techniques: Expressing, Western Blot, Over Expression, Migration, Transwell Assay, Software