Journal: Cerebral Cortex (New York, NY)
Article Title: Gene Expression Profiling of Preplate Neurons Destined for the Subplate: Genes Involved in Transcription, Axon Extension, Neurotransmitter Regulation, Steroid Hormone Signaling, and Neuronal Survival
Figure Lengend Snippet: Future subplate genes are expressed in RELN-negative preplate and subplate neurons. ( a ) Genes expressed in future subplate neurons categorized by function. ( b – h ) Double immunofluorescent staining for RELN (red) and marker proteins for future subplate candidate genes (green) in coronal cryostat sections of wild-type E12.5 ( b , c ) and E14.5 ( c – h ) brains. Hpca ( c , e ) and EAAC1 ( b , d ) are expressed in RELN-negative preplate cells and in subplate cell bodies. At E14.5, SRF ( f ) and RyR1 ( h ) are expressed in RELN-positive marginal zone neurons, as well as in intermediate zone fibers originating in the subplate, whereas Sez6 -driven EGFP ( g ) is expressed in subplate and cortical plate neurons, but not in RELN-positive cells. Scale bars = 50 μm.
Article Snippet: Primary antibodies used were: mouse anti-Cajal–Retzius cell marker reelin (RELN) (1:1000, MAB5364, Chemicon, Billerica, MA), rabbit anti-GFP (1:2000, Molecular Probes, Carlsbad, CA), rabbit anti-calretinin (1:2000, Swant, Bellinzona, Switzerland), rabbit anti-hippocalcin (1:2000, Abcam, Cambridge, MA), goat anti-EAAC1 (1:2000, Chemicon), rabbit anti-Ryr1 (1:1000, Chemicon), rabbit anti-serum response factor (SRF) (1:100, Abcam), rat anti-L1 (1:20, a gift from Dr J. Trotter), mouse IgM anti-TAG1 (1:2, gift from Dr Jane Dodd, Columbia University, NY).
Techniques: Staining, Marker