anti-rabbit igg Search Results


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  • 99
    Vector Laboratories antirabbit igg
    Antirabbit Igg, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 99/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antirabbit igg/product/Vector Laboratories
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    94
    Thermo Fisher donkey antirabbit igg
    Representative images of immuno-fluorescence staining of the microbeads at day 20. The images show that there were cell follicular structures inside the microbeads, which stained positive for both sodium-iodide symporter (NIS) (a) and thyroglobulin (Tg) (b). Cells were stained with DAPI only (c). Composite image including NIS, Tg and DAPI (d). Negative control of antigoat <t>IgG</t> (e). Negative control of <t>antirabbit</t> IgG (f). Thyroxine was detected in the lumen of follicular spheres (g). Negative controls of antimouse IgG (h). Scale bars on the images depict 20 µm. (A color version of this figure is available in the online journal.)
    Donkey Antirabbit Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/donkey antirabbit igg/product/Thermo Fisher
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    99
    Bio-Rad antirabbit igg
    Representative images of immuno-fluorescence staining of the microbeads at day 20. The images show that there were cell follicular structures inside the microbeads, which stained positive for both sodium-iodide symporter (NIS) (a) and thyroglobulin (Tg) (b). Cells were stained with DAPI only (c). Composite image including NIS, Tg and DAPI (d). Negative control of antigoat <t>IgG</t> (e). Negative control of <t>antirabbit</t> IgG (f). Thyroxine was detected in the lumen of follicular spheres (g). Negative controls of antimouse IgG (h). Scale bars on the images depict 20 µm. (A color version of this figure is available in the online journal.)
    Antirabbit Igg, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antirabbit igg/product/Bio-Rad
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    88
    Millipore antirabbit igg
    Representative images of immuno-fluorescence staining of the microbeads at day 20. The images show that there were cell follicular structures inside the microbeads, which stained positive for both sodium-iodide symporter (NIS) (a) and thyroglobulin (Tg) (b). Cells were stained with DAPI only (c). Composite image including NIS, Tg and DAPI (d). Negative control of antigoat <t>IgG</t> (e). Negative control of <t>antirabbit</t> IgG (f). Thyroxine was detected in the lumen of follicular spheres (g). Negative controls of antimouse IgG (h). Scale bars on the images depict 20 µm. (A color version of this figure is available in the online journal.)
    Antirabbit Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antirabbit igg/product/Millipore
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    95
    Abcam antirabbit igg antibody
    Representative images of immuno-fluorescence staining of the microbeads at day 20. The images show that there were cell follicular structures inside the microbeads, which stained positive for both sodium-iodide symporter (NIS) (a) and thyroglobulin (Tg) (b). Cells were stained with DAPI only (c). Composite image including NIS, Tg and DAPI (d). Negative control of antigoat <t>IgG</t> (e). Negative control of <t>antirabbit</t> IgG (f). Thyroxine was detected in the lumen of follicular spheres (g). Negative controls of antimouse IgG (h). Scale bars on the images depict 20 µm. (A color version of this figure is available in the online journal.)
    Antirabbit Igg Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antirabbit igg antibody/product/Abcam
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    92
    GE Healthcare anti rabbit igg antibodies
    Representative images of immuno-fluorescence staining of the microbeads at day 20. The images show that there were cell follicular structures inside the microbeads, which stained positive for both sodium-iodide symporter (NIS) (a) and thyroglobulin (Tg) (b). Cells were stained with DAPI only (c). Composite image including NIS, Tg and DAPI (d). Negative control of antigoat <t>IgG</t> (e). Negative control of <t>antirabbit</t> IgG (f). Thyroxine was detected in the lumen of follicular spheres (g). Negative controls of antimouse IgG (h). Scale bars on the images depict 20 µm. (A color version of this figure is available in the online journal.)
    Anti Rabbit Igg Antibodies, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 356 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Cell Signaling Technology Inc normal igg rabbit ab
    Representative images of immuno-fluorescence staining of the microbeads at day 20. The images show that there were cell follicular structures inside the microbeads, which stained positive for both sodium-iodide symporter (NIS) (a) and thyroglobulin (Tg) (b). Cells were stained with DAPI only (c). Composite image including NIS, Tg and DAPI (d). Negative control of antigoat <t>IgG</t> (e). Negative control of <t>antirabbit</t> IgG (f). Thyroxine was detected in the lumen of follicular spheres (g). Negative controls of antimouse IgG (h). Scale bars on the images depict 20 µm. (A color version of this figure is available in the online journal.)
    Normal Igg Rabbit Ab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    TaKaRa anti rabbit igg
    Representative images of immuno-fluorescence staining of the microbeads at day 20. The images show that there were cell follicular structures inside the microbeads, which stained positive for both sodium-iodide symporter (NIS) (a) and thyroglobulin (Tg) (b). Cells were stained with DAPI only (c). Composite image including NIS, Tg and DAPI (d). Negative control of antigoat <t>IgG</t> (e). Negative control of <t>antirabbit</t> IgG (f). Thyroxine was detected in the lumen of follicular spheres (g). Negative controls of antimouse IgG (h). Scale bars on the images depict 20 µm. (A color version of this figure is available in the online journal.)
    Anti Rabbit Igg, supplied by TaKaRa, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Nordic-Mubio anti rabbit igg
    Representative images of immuno-fluorescence staining of the microbeads at day 20. The images show that there were cell follicular structures inside the microbeads, which stained positive for both sodium-iodide symporter (NIS) (a) and thyroglobulin (Tg) (b). Cells were stained with DAPI only (c). Composite image including NIS, Tg and DAPI (d). Negative control of antigoat <t>IgG</t> (e). Negative control of <t>antirabbit</t> IgG (f). Thyroxine was detected in the lumen of follicular spheres (g). Negative controls of antimouse IgG (h). Scale bars on the images depict 20 µm. (A color version of this figure is available in the online journal.)
    Anti Rabbit Igg, supplied by Nordic-Mubio, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Santa Cruz Biotechnology anti rabbit igg antibody
    Representative images of immuno-fluorescence staining of the microbeads at day 20. The images show that there were cell follicular structures inside the microbeads, which stained positive for both sodium-iodide symporter (NIS) (a) and thyroglobulin (Tg) (b). Cells were stained with DAPI only (c). Composite image including NIS, Tg and DAPI (d). Negative control of antigoat <t>IgG</t> (e). Negative control of <t>antirabbit</t> IgG (f). Thyroxine was detected in the lumen of follicular spheres (g). Negative controls of antimouse IgG (h). Scale bars on the images depict 20 µm. (A color version of this figure is available in the online journal.)
    Anti Rabbit Igg Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 348 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Jackson Immuno antirabbit igg
    Representative images of immuno-fluorescence staining of the microbeads at day 20. The images show that there were cell follicular structures inside the microbeads, which stained positive for both sodium-iodide symporter (NIS) (a) and thyroglobulin (Tg) (b). Cells were stained with DAPI only (c). Composite image including NIS, Tg and DAPI (d). Negative control of antigoat <t>IgG</t> (e). Negative control of <t>antirabbit</t> IgG (f). Thyroxine was detected in the lumen of follicular spheres (g). Negative controls of antimouse IgG (h). Scale bars on the images depict 20 µm. (A color version of this figure is available in the online journal.)
    Antirabbit Igg, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 97/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore goat anti rabbit igg
    Association of leureptin with hemocyte membranes Hemocytes collected 24 h after injection of saline or M. luteus were fixed on glass slides. Anti-leureptin <t>IgG</t> and <t>FITC-labeled</t> goat-anti-rabbit antibody were used to detect leureptin as described in materials and methods. The upper panels show hemocytes viewed by phase contrast, and the lower panels show hemocytes viewed by fluorescence microscopy. Both plasmatocytes (indicated by arrows ) and granulocytes stained more intensely in bacteria-challenged insects. Oenocytoids had similar labeling intensity in control and treated samples.
    Goat Anti Rabbit Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 3875 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Cell Signaling Technology Inc antirabbit igg
    Association of leureptin with hemocyte membranes Hemocytes collected 24 h after injection of saline or M. luteus were fixed on glass slides. Anti-leureptin <t>IgG</t> and <t>FITC-labeled</t> goat-anti-rabbit antibody were used to detect leureptin as described in materials and methods. The upper panels show hemocytes viewed by phase contrast, and the lower panels show hemocytes viewed by fluorescence microscopy. Both plasmatocytes (indicated by arrows ) and granulocytes stained more intensely in bacteria-challenged insects. Oenocytoids had similar labeling intensity in control and treated samples.
    Antirabbit Igg, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 179 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antirabbit igg/product/Cell Signaling Technology Inc
    Average 94 stars, based on 179 article reviews
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    antirabbit igg - by Bioz Stars, 2020-07
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    92
    Bio-Rad antibodies goat antirabbit igg
    Association of leureptin with hemocyte membranes Hemocytes collected 24 h after injection of saline or M. luteus were fixed on glass slides. Anti-leureptin <t>IgG</t> and <t>FITC-labeled</t> goat-anti-rabbit antibody were used to detect leureptin as described in materials and methods. The upper panels show hemocytes viewed by phase contrast, and the lower panels show hemocytes viewed by fluorescence microscopy. Both plasmatocytes (indicated by arrows ) and granulocytes stained more intensely in bacteria-challenged insects. Oenocytoids had similar labeling intensity in control and treated samples.
    Antibodies Goat Antirabbit Igg, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/antibodies goat antirabbit igg/product/Bio-Rad
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    99
    Millipore antirabbit igg hrp
    Association of leureptin with hemocyte membranes Hemocytes collected 24 h after injection of saline or M. luteus were fixed on glass slides. Anti-leureptin <t>IgG</t> and <t>FITC-labeled</t> goat-anti-rabbit antibody were used to detect leureptin as described in materials and methods. The upper panels show hemocytes viewed by phase contrast, and the lower panels show hemocytes viewed by fluorescence microscopy. Both plasmatocytes (indicated by arrows ) and granulocytes stained more intensely in bacteria-challenged insects. Oenocytoids had similar labeling intensity in control and treated samples.
    Antirabbit Igg Hrp, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Thermo Fisher anti rabbit igg
    Physical exercise and intracarotid injection of IGF-I produce similar effects in the brain. A , The same brain areas show labeling of neurons with IGF-I after treadmill running ( a–c ) and intracarotid injection of IGF-I ( d–f ). Three representative areas are shown. Nonexercised, saline-injected rats show almost undetectable brain IGF-I staining ( B ). Str , Striatum; Cx , cerebral cortex; RN , red nucleus. <t>Biotinylated</t> anti-rabbit <t>IgG</t> followed by Cy3-streptavidin was used after incubation with a polyclonal anti-IGF-I antibody. B , Digoxigenin ( DIG ) and IGF-I colocalize within the same neurons after intracarotid injection of DIG–IGF-I. A representative field in the brainstem is shown. a , Low magnification (10×) of IGF-I staining in the inferior olive nucleus ( IO ) of a saline-injected rat. Note the absence of signal. Inset , Higher magnification (40×) of the IO field. b , The same field showing IGF-I staining in an IGF-I-injected rat. Inset , High magnification showing IGF-I-positive cells. c , High magnification (40×) of IO neurons stained with a monoclonal anti-DIG antibody ( green ). d , The same field stained with a polyclonal anti-IGF-I antibody ( red ). e , Colocalization of DIG and IGF-I within the same IO neurons. Scale bars: a , b , 500 μm; c – e , 50 μm. Primary antibody incubation was followed by an anti-rabbit Cy2 and anti-mouse Cy5, respectively. C , Exercise or intracarotid injection of IGF-I elicits a similar pattern of increased c-Fos staining throughout the brain. Only the piriform cortex ( Pir ) is shown as a representative area. a , Control animals show no c-Fos staining. b , c-Fos staining after 1 hr of intracarotid injection of IGF-I. c , c-Fos staining after 1 hr of running. Scale bar ( a – c ): 500 μm. d , Higher magnification of the field in c showing nuclear localization of the c-Fos signal. Scale bar, 50 μm. Arrows indicate immunoreactive cells. A monoclonal anti-c-Fos antibody followed by a biotinylated anti-mouse IgG and Cy3-streptavidin was used. D , Blockade of the exercise-induced capture of IGF-I by brain cells results in absence of c-Fos labeling after exercise. a , IGF-I labeling in the hippocampus of a rat that ran for 1 hr. c , Chronic intracerebroventricular delivery of a combination of an anti-IGF-I antibody and an IGF-I receptor antagonist results in absence of IGF-I staining after 1 hr of running exercise. Scale bar ( a , c ): 50 μm. b , c-Fos staining is induced in the hippocampus by 1 hr of running. c , No c-Fos labeling is seen in exercised animals in which brain uptake of IGF-I is blocked. Scale bar ( b , d ): 500 μm. The hippocampus is shown as a representative area, but absence of labeling for IGF-I and c-Fos was found in all brain areas. E , Expression of BDNF in the hippocampus is increased by running and by intracarotid injection of IGF-I. Control: background BDNF RNA staining in brain slices incubated with excess unlabeled probe. Saline: animals injected with saline show weak BDNF expression in the hippocampus. Exercise: running induces increased expression of BDNF in the hippocampus. IGF-I: injection of IGF-I produces a similar increase in hippocampal expression of BDNF. Cx , Cortex; Hy , hippocampus.
    Anti Rabbit Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 7812 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti rabbit igg/product/Thermo Fisher
    Average 95 stars, based on 7812 article reviews
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    Image Search Results


    Representative images of immuno-fluorescence staining of the microbeads at day 20. The images show that there were cell follicular structures inside the microbeads, which stained positive for both sodium-iodide symporter (NIS) (a) and thyroglobulin (Tg) (b). Cells were stained with DAPI only (c). Composite image including NIS, Tg and DAPI (d). Negative control of antigoat IgG (e). Negative control of antirabbit IgG (f). Thyroxine was detected in the lumen of follicular spheres (g). Negative controls of antimouse IgG (h). Scale bars on the images depict 20 µm. (A color version of this figure is available in the online journal.)

    Journal: Experimental Biology and Medicine

    Article Title: Microencapsulation of porcine thyroid cell organoids within a polymer microcapsule construct

    doi: 10.1177/1535370216673746

    Figure Lengend Snippet: Representative images of immuno-fluorescence staining of the microbeads at day 20. The images show that there were cell follicular structures inside the microbeads, which stained positive for both sodium-iodide symporter (NIS) (a) and thyroglobulin (Tg) (b). Cells were stained with DAPI only (c). Composite image including NIS, Tg and DAPI (d). Negative control of antigoat IgG (e). Negative control of antirabbit IgG (f). Thyroxine was detected in the lumen of follicular spheres (g). Negative controls of antimouse IgG (h). Scale bars on the images depict 20 µm. (A color version of this figure is available in the online journal.)

    Article Snippet: The cells were then incubated with a mixed solution of donkey antigoat IgG (Alexa Fluor® 594-conjugated; Invitrogen, Grand Island, NY) and donkey antirabbit IgG (Alexa Fluor® 488-conjugated; Invitrogen) for 60 min in the dark.

    Techniques: Fluorescence, Staining, Negative Control

    Representative images of light microscopic observation and immuno-fluorescence staining of primary cultured porcine thyroid cells. (a) The monolayer porcine thyroid cells in culture after two days of incubation. (b) The thyroid cells with the hemisphere-like domes formed in culture after four days of incubation. (a and b) Scale bar = 100 µm. (c) Negative control for antirabbit IgG. (d) Negative control for antigoat IgG. (e) Positive staining of sodium-iodide symporter (NIS). (f) Positive staining of thyroglobulin (Tg). (g) Composite picture of NIS, Tg, and DAPI. (c to g) Scale bar = 50 µm. (A color version of this figure is available in the online journal.)

    Journal: Experimental Biology and Medicine

    Article Title: Microencapsulation of porcine thyroid cell organoids within a polymer microcapsule construct

    doi: 10.1177/1535370216673746

    Figure Lengend Snippet: Representative images of light microscopic observation and immuno-fluorescence staining of primary cultured porcine thyroid cells. (a) The monolayer porcine thyroid cells in culture after two days of incubation. (b) The thyroid cells with the hemisphere-like domes formed in culture after four days of incubation. (a and b) Scale bar = 100 µm. (c) Negative control for antirabbit IgG. (d) Negative control for antigoat IgG. (e) Positive staining of sodium-iodide symporter (NIS). (f) Positive staining of thyroglobulin (Tg). (g) Composite picture of NIS, Tg, and DAPI. (c to g) Scale bar = 50 µm. (A color version of this figure is available in the online journal.)

    Article Snippet: The cells were then incubated with a mixed solution of donkey antigoat IgG (Alexa Fluor® 594-conjugated; Invitrogen, Grand Island, NY) and donkey antirabbit IgG (Alexa Fluor® 488-conjugated; Invitrogen) for 60 min in the dark.

    Techniques: Fluorescence, Staining, Cell Culture, Incubation, Negative Control

    Association of leureptin with hemocyte membranes Hemocytes collected 24 h after injection of saline or M. luteus were fixed on glass slides. Anti-leureptin IgG and FITC-labeled goat-anti-rabbit antibody were used to detect leureptin as described in materials and methods. The upper panels show hemocytes viewed by phase contrast, and the lower panels show hemocytes viewed by fluorescence microscopy. Both plasmatocytes (indicated by arrows ) and granulocytes stained more intensely in bacteria-challenged insects. Oenocytoids had similar labeling intensity in control and treated samples.

    Journal: Insect biochemistry and molecular biology

    Article Title: Leureptin: a soluble, extracellular leucine-rich repeat protein from Manduca sexta that binds lipopolysaccharide

    doi: 10.1016/j.ibmb.2010.07.002

    Figure Lengend Snippet: Association of leureptin with hemocyte membranes Hemocytes collected 24 h after injection of saline or M. luteus were fixed on glass slides. Anti-leureptin IgG and FITC-labeled goat-anti-rabbit antibody were used to detect leureptin as described in materials and methods. The upper panels show hemocytes viewed by phase contrast, and the lower panels show hemocytes viewed by fluorescence microscopy. Both plasmatocytes (indicated by arrows ) and granulocytes stained more intensely in bacteria-challenged insects. Oenocytoids had similar labeling intensity in control and treated samples.

    Article Snippet: Slides were then washed with 40 μl TBS/well three times for 5 min each at room temperature, followed by adding 30 μl of the secondary antibody, fluorescein isothiocyanate (FITC) labeled goat-anti-rabbit- IgG (Sigma), diluted at 1:400 in TBS containing 1% BSA.

    Techniques: Injection, Labeling, Fluorescence, Microscopy, Staining

    Physical exercise and intracarotid injection of IGF-I produce similar effects in the brain. A , The same brain areas show labeling of neurons with IGF-I after treadmill running ( a–c ) and intracarotid injection of IGF-I ( d–f ). Three representative areas are shown. Nonexercised, saline-injected rats show almost undetectable brain IGF-I staining ( B ). Str , Striatum; Cx , cerebral cortex; RN , red nucleus. Biotinylated anti-rabbit IgG followed by Cy3-streptavidin was used after incubation with a polyclonal anti-IGF-I antibody. B , Digoxigenin ( DIG ) and IGF-I colocalize within the same neurons after intracarotid injection of DIG–IGF-I. A representative field in the brainstem is shown. a , Low magnification (10×) of IGF-I staining in the inferior olive nucleus ( IO ) of a saline-injected rat. Note the absence of signal. Inset , Higher magnification (40×) of the IO field. b , The same field showing IGF-I staining in an IGF-I-injected rat. Inset , High magnification showing IGF-I-positive cells. c , High magnification (40×) of IO neurons stained with a monoclonal anti-DIG antibody ( green ). d , The same field stained with a polyclonal anti-IGF-I antibody ( red ). e , Colocalization of DIG and IGF-I within the same IO neurons. Scale bars: a , b , 500 μm; c – e , 50 μm. Primary antibody incubation was followed by an anti-rabbit Cy2 and anti-mouse Cy5, respectively. C , Exercise or intracarotid injection of IGF-I elicits a similar pattern of increased c-Fos staining throughout the brain. Only the piriform cortex ( Pir ) is shown as a representative area. a , Control animals show no c-Fos staining. b , c-Fos staining after 1 hr of intracarotid injection of IGF-I. c , c-Fos staining after 1 hr of running. Scale bar ( a – c ): 500 μm. d , Higher magnification of the field in c showing nuclear localization of the c-Fos signal. Scale bar, 50 μm. Arrows indicate immunoreactive cells. A monoclonal anti-c-Fos antibody followed by a biotinylated anti-mouse IgG and Cy3-streptavidin was used. D , Blockade of the exercise-induced capture of IGF-I by brain cells results in absence of c-Fos labeling after exercise. a , IGF-I labeling in the hippocampus of a rat that ran for 1 hr. c , Chronic intracerebroventricular delivery of a combination of an anti-IGF-I antibody and an IGF-I receptor antagonist results in absence of IGF-I staining after 1 hr of running exercise. Scale bar ( a , c ): 50 μm. b , c-Fos staining is induced in the hippocampus by 1 hr of running. c , No c-Fos labeling is seen in exercised animals in which brain uptake of IGF-I is blocked. Scale bar ( b , d ): 500 μm. The hippocampus is shown as a representative area, but absence of labeling for IGF-I and c-Fos was found in all brain areas. E , Expression of BDNF in the hippocampus is increased by running and by intracarotid injection of IGF-I. Control: background BDNF RNA staining in brain slices incubated with excess unlabeled probe. Saline: animals injected with saline show weak BDNF expression in the hippocampus. Exercise: running induces increased expression of BDNF in the hippocampus. IGF-I: injection of IGF-I produces a similar increase in hippocampal expression of BDNF. Cx , Cortex; Hy , hippocampus.

    Journal: The Journal of Neuroscience

    Article Title: Circulating Insulin-Like Growth Factor I Mediates Effects of Exercise on the Brain

    doi: 10.1523/JNEUROSCI.20-08-02926.2000

    Figure Lengend Snippet: Physical exercise and intracarotid injection of IGF-I produce similar effects in the brain. A , The same brain areas show labeling of neurons with IGF-I after treadmill running ( a–c ) and intracarotid injection of IGF-I ( d–f ). Three representative areas are shown. Nonexercised, saline-injected rats show almost undetectable brain IGF-I staining ( B ). Str , Striatum; Cx , cerebral cortex; RN , red nucleus. Biotinylated anti-rabbit IgG followed by Cy3-streptavidin was used after incubation with a polyclonal anti-IGF-I antibody. B , Digoxigenin ( DIG ) and IGF-I colocalize within the same neurons after intracarotid injection of DIG–IGF-I. A representative field in the brainstem is shown. a , Low magnification (10×) of IGF-I staining in the inferior olive nucleus ( IO ) of a saline-injected rat. Note the absence of signal. Inset , Higher magnification (40×) of the IO field. b , The same field showing IGF-I staining in an IGF-I-injected rat. Inset , High magnification showing IGF-I-positive cells. c , High magnification (40×) of IO neurons stained with a monoclonal anti-DIG antibody ( green ). d , The same field stained with a polyclonal anti-IGF-I antibody ( red ). e , Colocalization of DIG and IGF-I within the same IO neurons. Scale bars: a , b , 500 μm; c – e , 50 μm. Primary antibody incubation was followed by an anti-rabbit Cy2 and anti-mouse Cy5, respectively. C , Exercise or intracarotid injection of IGF-I elicits a similar pattern of increased c-Fos staining throughout the brain. Only the piriform cortex ( Pir ) is shown as a representative area. a , Control animals show no c-Fos staining. b , c-Fos staining after 1 hr of intracarotid injection of IGF-I. c , c-Fos staining after 1 hr of running. Scale bar ( a – c ): 500 μm. d , Higher magnification of the field in c showing nuclear localization of the c-Fos signal. Scale bar, 50 μm. Arrows indicate immunoreactive cells. A monoclonal anti-c-Fos antibody followed by a biotinylated anti-mouse IgG and Cy3-streptavidin was used. D , Blockade of the exercise-induced capture of IGF-I by brain cells results in absence of c-Fos labeling after exercise. a , IGF-I labeling in the hippocampus of a rat that ran for 1 hr. c , Chronic intracerebroventricular delivery of a combination of an anti-IGF-I antibody and an IGF-I receptor antagonist results in absence of IGF-I staining after 1 hr of running exercise. Scale bar ( a , c ): 50 μm. b , c-Fos staining is induced in the hippocampus by 1 hr of running. c , No c-Fos labeling is seen in exercised animals in which brain uptake of IGF-I is blocked. Scale bar ( b , d ): 500 μm. The hippocampus is shown as a representative area, but absence of labeling for IGF-I and c-Fos was found in all brain areas. E , Expression of BDNF in the hippocampus is increased by running and by intracarotid injection of IGF-I. Control: background BDNF RNA staining in brain slices incubated with excess unlabeled probe. Saline: animals injected with saline show weak BDNF expression in the hippocampus. Exercise: running induces increased expression of BDNF in the hippocampus. IGF-I: injection of IGF-I produces a similar increase in hippocampal expression of BDNF. Cx , Cortex; Hy , hippocampus.

    Article Snippet: The secondary antibodies that were used were biotinylated goat anti-mouse IgG (1:1000) (Jackson ImmunoResearch, West Grove, PA) or anti-rabbit IgG (1:250–1:1000) (Pierce, Rockford, IL).

    Techniques: Injection, Labeling, Staining, Incubation, Expressing