anti-ova antibodies Search Results


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  • 92
    Thermo Fisher rabbit anti ovalbumin ova antibody
    Rabbit Anti Ovalbumin Ova Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore polyclonal rabbit anti ova anti ova antibody
    Polyclonal Rabbit Anti Ova Anti Ova Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Abcam anti ovalbumin antibody
    Anti Ovalbumin Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Biomol GmbH anti ovalbumin anti ova antibody
    Anti Ovalbumin Anti Ova Antibody, supplied by Biomol GmbH, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Abcam anti ova secondary antibodies
    Fructus Arctii extract increased TEER value and decreased <t>OVA</t> influx in Caco-2 cells. The monolayers of Caco-2 cells were preincubated with HBSS for 30 min at 37°C in a CO 2 incubator to stabilize the cell monolayers. The cell monolayers were treated with each sample and bile salts for 60 min, and OVA was added for 3 h at 37°C. (a) Changes of TEER were measured by treating the cell monolayers with nine extracts (400 μ g/mL) and (b) Fructus Arctii extract (FAE) at various doses (100, 200, and 400 μ g/mL) for 3 h. (c and d) Changes in TEER value and OVA influx induced by FAE were detected by <t>ELISA.</t> Each value is presented as mean ± SD ( n = 3). Bars are significantly different from the control at ∗ P
    Anti Ova Secondary Antibodies, supplied by Abcam, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Abcam anti ova
    Antibody and T cell responses following vaccination with <t>OVA</t> targeted to MHC class II molecules or CCR1/3/5. (a,b) Supernatants of 293E cells transfected with the indicated plasmids were tested for secreted proteins in ELISA (a) and examined by Western blotting with anti-OVA mAb under reducing (+ME) or non-reducing (-ME) conditions (b). Vaccine proteins are indicated below lanes. (c-f) Mice were immunized once i.d. with 25 µg DNA/EP, as indicated. (c-e) Sera were assayed for total <t>IgG</t> (c), IgG1 (d) or IgG2a (e) against OVA. (f) Splenocytes collected at day 14 post immunization were stimulated in vitro with OVA protein or controls as indicated, and analyzed by an IFNγ EliSpot. *indicates p
    Anti Ova, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Lifespan Biosciences anti ova antibody
    Antibody and T cell responses following vaccination with <t>OVA</t> targeted to MHC class II molecules or CCR1/3/5. (a,b) Supernatants of 293E cells transfected with the indicated plasmids were tested for secreted proteins in ELISA (a) and examined by Western blotting with anti-OVA mAb under reducing (+ME) or non-reducing (-ME) conditions (b). Vaccine proteins are indicated below lanes. (c-f) Mice were immunized once i.d. with 25 µg DNA/EP, as indicated. (c-e) Sera were assayed for total <t>IgG</t> (c), IgG1 (d) or IgG2a (e) against OVA. (f) Splenocytes collected at day 14 post immunization were stimulated in vitro with OVA protein or controls as indicated, and analyzed by an IFNγ EliSpot. *indicates p
    Anti Ova Antibody, supplied by Lifespan Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Delta Biolabs anti ova antibodies
    Antibody and T cell responses following vaccination with <t>OVA</t> targeted to MHC class II molecules or CCR1/3/5. (a,b) Supernatants of 293E cells transfected with the indicated plasmids were tested for secreted proteins in ELISA (a) and examined by Western blotting with anti-OVA mAb under reducing (+ME) or non-reducing (-ME) conditions (b). Vaccine proteins are indicated below lanes. (c-f) Mice were immunized once i.d. with 25 µg DNA/EP, as indicated. (c-e) Sera were assayed for total <t>IgG</t> (c), IgG1 (d) or IgG2a (e) against OVA. (f) Splenocytes collected at day 14 post immunization were stimulated in vitro with OVA protein or controls as indicated, and analyzed by an IFNγ EliSpot. *indicates p
    Anti Ova Antibodies, supplied by Delta Biolabs, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Bio-Rad mouse anti ova capture antibody
    Antibody and T cell responses following vaccination with <t>OVA</t> targeted to MHC class II molecules or CCR1/3/5. (a,b) Supernatants of 293E cells transfected with the indicated plasmids were tested for secreted proteins in ELISA (a) and examined by Western blotting with anti-OVA mAb under reducing (+ME) or non-reducing (-ME) conditions (b). Vaccine proteins are indicated below lanes. (c-f) Mice were immunized once i.d. with 25 µg DNA/EP, as indicated. (c-e) Sera were assayed for total <t>IgG</t> (c), IgG1 (d) or IgG2a (e) against OVA. (f) Splenocytes collected at day 14 post immunization were stimulated in vitro with OVA protein or controls as indicated, and analyzed by an IFNγ EliSpot. *indicates p
    Mouse Anti Ova Capture Antibody, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Chondrex inc anti ovalbumin ova ige antibody
    Bone marrow-derived cultured mast cells (BMMCs) produce and secrete interleukin (IL)-33 through <t>IgE-mediated</t> activation. (a) Four-week BMMCs were stained with FcɛRI, c-kit, and CD49b, and then detected by flow cytometry. Ninety-five percent of BMMCs were FcɛRI + c-kit + CD49b − . (b) Toluidine blue staining of 4-week mast cells. (c) IL-33 mRNA kinetic expression. Ovalbumin <t>(OVA)-specific</t> IgE Ab (E-C1) sensitized mast cells stimulated with 10 μg/mL OVA for different time periods. (d) IL-33 mRNA dose response. OVA-specific IgE Ab (E-C1) sensitized mast cells stimulated with different dose of OVA for 3 h. (e) Intracellular IL-33 expression was measured by flow cytometry for the following comparison: with or without antigen challenge and with or without antigen plus monensin. (f) Western blotting analysis of IL-33 protein expression at 8 h and 24 h following OVA challenge. Below, band intensities normalized to actin and relative to the medium (M) control group were calculated with Quantity One software (Bio-Rad). (g) IL-33 secretions from E-C1 sensitized mast cells with or without OVA stimulation detected by cell-based enzyme-linked immunosorbent assay (ELISA). Data are representative of 4 independent experiments. * P
    Anti Ovalbumin Ova Ige Antibody, supplied by Chondrex inc, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore anti ova antibody
    Concurrent exposure to IGF2 and specific antigens induces IL-10 promoter demethylation. OVAsBCs were prepared and cultured in the presence of IGF2 and/or <t>OVA</t> for 72 h in the presence or absence of <t>5-azacytidine</t> ( 5-aza ; a DNA methyltransferase inhibitor) or saline (control). The treatment is denoted in each panel. A and B , the immunoblots indicate the levels of IL-10 in OVAsBCs. C , IL-10 promoter methylation in OVAsBCs was assessed by methylation-specific PCR. D , the bars indicate the percentage of demethylated DNA (calculated with the data of C ). E , the immunoblots show the STAT5 gene silencing. M , methylated; U , unmethylated. shRNA STAT5 , OVAsBCs treated by STAT5 gene silencing. cshRNA , OVAsBCs treated by control shRNA. The data represent three separate experiments. Error bars represent S.D. *, p
    Anti Ova Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Nordic BioSite mouse anti ova antibody
    Concurrent exposure to IGF2 and specific antigens induces IL-10 promoter demethylation. OVAsBCs were prepared and cultured in the presence of IGF2 and/or <t>OVA</t> for 72 h in the presence or absence of <t>5-azacytidine</t> ( 5-aza ; a DNA methyltransferase inhibitor) or saline (control). The treatment is denoted in each panel. A and B , the immunoblots indicate the levels of IL-10 in OVAsBCs. C , IL-10 promoter methylation in OVAsBCs was assessed by methylation-specific PCR. D , the bars indicate the percentage of demethylated DNA (calculated with the data of C ). E , the immunoblots show the STAT5 gene silencing. M , methylated; U , unmethylated. shRNA STAT5 , OVAsBCs treated by STAT5 gene silencing. cshRNA , OVAsBCs treated by control shRNA. The data represent three separate experiments. Error bars represent S.D. *, p
    Mouse Anti Ova Antibody, supplied by Nordic BioSite, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore anti ova sigma antibodies
    Induction of autoantibodies and proteinuria. BALB/c mice were repeatedly injected i.p. with 25 µg SEB, 500 µg <t>OVA</t> or PBS every 5 d. (A) Serum <t>IgG-</t> and IgM-RFs, anti-galactose-deficient IgG and anti-Sm antibodies were measured using ELISA. The arbitrary unit (AU) of 1.0 is equivalent to the titer obtained from sera of prototypic autoimmune MRL/lpr mice. Data from each mouse are connected by dotted lines. (B) Adoptive transfer of splenic B, T, CD4 + T or CD8 + T cells of SEB-, OVA- or PBS-immunized BALB/c mice into naïve BALB/c mice. The recipient mice were given single i.p. injection of 25 µg SEB or 500 µg OVA 24 h after cell transfer, and autoantibodies were measured 2 weeks later. (C) Adoptive transfer of cells from OVA-immunized BALB/c mice into β 2 m-deficient mice.
    Anti Ova Sigma Antibodies, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Rockland Immunochemicals rabbit anti ova antibody
    Induction of autoantibodies and proteinuria. BALB/c mice were repeatedly injected i.p. with 25 µg SEB, 500 µg <t>OVA</t> or PBS every 5 d. (A) Serum <t>IgG-</t> and IgM-RFs, anti-galactose-deficient IgG and anti-Sm antibodies were measured using ELISA. The arbitrary unit (AU) of 1.0 is equivalent to the titer obtained from sera of prototypic autoimmune MRL/lpr mice. Data from each mouse are connected by dotted lines. (B) Adoptive transfer of splenic B, T, CD4 + T or CD8 + T cells of SEB-, OVA- or PBS-immunized BALB/c mice into naïve BALB/c mice. The recipient mice were given single i.p. injection of 25 µg SEB or 500 µg OVA 24 h after cell transfer, and autoantibodies were measured 2 weeks later. (C) Adoptive transfer of cells from OVA-immunized BALB/c mice into β 2 m-deficient mice.
    Rabbit Anti Ova Antibody, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Antibody Research Corporation rabbit anti ova antibody
    Induction of autoantibodies and proteinuria. BALB/c mice were repeatedly injected i.p. with 25 µg SEB, 500 µg <t>OVA</t> or PBS every 5 d. (A) Serum <t>IgG-</t> and IgM-RFs, anti-galactose-deficient IgG and anti-Sm antibodies were measured using ELISA. The arbitrary unit (AU) of 1.0 is equivalent to the titer obtained from sera of prototypic autoimmune MRL/lpr mice. Data from each mouse are connected by dotted lines. (B) Adoptive transfer of splenic B, T, CD4 + T or CD8 + T cells of SEB-, OVA- or PBS-immunized BALB/c mice into naïve BALB/c mice. The recipient mice were given single i.p. injection of 25 µg SEB or 500 µg OVA 24 h after cell transfer, and autoantibodies were measured 2 weeks later. (C) Adoptive transfer of cells from OVA-immunized BALB/c mice into β 2 m-deficient mice.
    Rabbit Anti Ova Antibody, supplied by Antibody Research Corporation, used in various techniques. Bioz Stars score: 88/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore polyclonal anti ova antibody
    Induction of autoantibodies and proteinuria. BALB/c mice were repeatedly injected i.p. with 25 µg SEB, 500 µg <t>OVA</t> or PBS every 5 d. (A) Serum <t>IgG-</t> and IgM-RFs, anti-galactose-deficient IgG and anti-Sm antibodies were measured using ELISA. The arbitrary unit (AU) of 1.0 is equivalent to the titer obtained from sera of prototypic autoimmune MRL/lpr mice. Data from each mouse are connected by dotted lines. (B) Adoptive transfer of splenic B, T, CD4 + T or CD8 + T cells of SEB-, OVA- or PBS-immunized BALB/c mice into naïve BALB/c mice. The recipient mice were given single i.p. injection of 25 µg SEB or 500 µg OVA 24 h after cell transfer, and autoantibodies were measured 2 weeks later. (C) Adoptive transfer of cells from OVA-immunized BALB/c mice into β 2 m-deficient mice.
    Polyclonal Anti Ova Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Santa Cruz Biotechnology anti ova monoclonal antibody 3g2e1d9
    Cyclic peptide CsA mediated inhibition of <t>OVA</t> amyloid synthesis as revealed by fluoresense microscopy. Green fluorescence associated with OVA amyloid bound ThT was visualized employing epifluoresense microscopy. The epi-fluoprescence micrographs of the as-synthesized OVA amyloid formed in absenccse ( A ) or presense ( B ) of 500 nM CsA. The panel (C) corresponds to Western blot profile of amyloid intermediates formed during fibril formation. Western blot analysis of OVA-amyloid probed with OVA specific <t>3G2E1D9</t> monoclonal antibodies reveals presence of monomeric, dimeric as well as oligomeric OVA (lane i) formed at the beginning of exponential phase of OVA aggregation process (without CsA). Lane ii shows inhibitory effect CsA (500 nM) on the formation of monomeric and dimeric intermediates of the OVA.
    Anti Ova Monoclonal Antibody 3g2e1d9, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Fructus Arctii extract increased TEER value and decreased OVA influx in Caco-2 cells. The monolayers of Caco-2 cells were preincubated with HBSS for 30 min at 37°C in a CO 2 incubator to stabilize the cell monolayers. The cell monolayers were treated with each sample and bile salts for 60 min, and OVA was added for 3 h at 37°C. (a) Changes of TEER were measured by treating the cell monolayers with nine extracts (400 μ g/mL) and (b) Fructus Arctii extract (FAE) at various doses (100, 200, and 400 μ g/mL) for 3 h. (c and d) Changes in TEER value and OVA influx induced by FAE were detected by ELISA. Each value is presented as mean ± SD ( n = 3). Bars are significantly different from the control at ∗ P

    Journal: Evidence-based Complementary and Alternative Medicine : eCAM

    Article Title: Arctigenin from Fructus Arctii (Seed of Burdock) Reinforces Intestinal Barrier Function in Caco-2 Cell Monolayers

    doi: 10.1155/2015/368105

    Figure Lengend Snippet: Fructus Arctii extract increased TEER value and decreased OVA influx in Caco-2 cells. The monolayers of Caco-2 cells were preincubated with HBSS for 30 min at 37°C in a CO 2 incubator to stabilize the cell monolayers. The cell monolayers were treated with each sample and bile salts for 60 min, and OVA was added for 3 h at 37°C. (a) Changes of TEER were measured by treating the cell monolayers with nine extracts (400 μ g/mL) and (b) Fructus Arctii extract (FAE) at various doses (100, 200, and 400 μ g/mL) for 3 h. (c and d) Changes in TEER value and OVA influx induced by FAE were detected by ELISA. Each value is presented as mean ± SD ( n = 3). Bars are significantly different from the control at ∗ P

    Article Snippet: ELISA Coating anti-OVA primary antibodies (ab17290) and horseradish peroxidase- (HRP-) conjugated anti-OVA secondary antibodies (ab20415) for ELISA were purchased from Abcam (Cambridge, MA, USA).

    Techniques: Enzyme-linked Immunosorbent Assay

    Antibody and T cell responses following vaccination with OVA targeted to MHC class II molecules or CCR1/3/5. (a,b) Supernatants of 293E cells transfected with the indicated plasmids were tested for secreted proteins in ELISA (a) and examined by Western blotting with anti-OVA mAb under reducing (+ME) or non-reducing (-ME) conditions (b). Vaccine proteins are indicated below lanes. (c-f) Mice were immunized once i.d. with 25 µg DNA/EP, as indicated. (c-e) Sera were assayed for total IgG (c), IgG1 (d) or IgG2a (e) against OVA. (f) Splenocytes collected at day 14 post immunization were stimulated in vitro with OVA protein or controls as indicated, and analyzed by an IFNγ EliSpot. *indicates p

    Journal: PLoS ONE

    Article Title: The Specificity of Targeted Vaccines for APC Surface Molecules Influences the Immune Response Phenotype

    doi: 10.1371/journal.pone.0080008

    Figure Lengend Snippet: Antibody and T cell responses following vaccination with OVA targeted to MHC class II molecules or CCR1/3/5. (a,b) Supernatants of 293E cells transfected with the indicated plasmids were tested for secreted proteins in ELISA (a) and examined by Western blotting with anti-OVA mAb under reducing (+ME) or non-reducing (-ME) conditions (b). Vaccine proteins are indicated below lanes. (c-f) Mice were immunized once i.d. with 25 µg DNA/EP, as indicated. (c-e) Sera were assayed for total IgG (c), IgG1 (d) or IgG2a (e) against OVA. (f) Splenocytes collected at day 14 post immunization were stimulated in vitro with OVA protein or controls as indicated, and analyzed by an IFNγ EliSpot. *indicates p

    Article Snippet: Prior to assaying, the harvested supernatants were centrifuged at 13 000 rpm for 4 min. For Western blotting, vaccine proteins were run on a Novex 4–12% Tris-Glycine gel (Invitrogen) together with a SeeBlue Plus2 Prestained Standard (LC5925, Invitrogen), blotted (Immun-Blot PVDF membrane, 162–0177, BioRad) and incubated with a biotinylated anti-HA antibody (H36-4-52, kind gift from Siegfried Weiss) and Streptavidin-HRP (RPN1231V, GE Healthcare), or anti-OVA (ab17293, Abcam) and anti-mouse IgG-HRP (1030-05, Southern Biotech).

    Techniques: Transfection, Enzyme-linked Immunosorbent Assay, Western Blot, Mouse Assay, In Vitro, Enzyme-linked Immunospot

    Bone marrow-derived cultured mast cells (BMMCs) produce and secrete interleukin (IL)-33 through IgE-mediated activation. (a) Four-week BMMCs were stained with FcɛRI, c-kit, and CD49b, and then detected by flow cytometry. Ninety-five percent of BMMCs were FcɛRI + c-kit + CD49b − . (b) Toluidine blue staining of 4-week mast cells. (c) IL-33 mRNA kinetic expression. Ovalbumin (OVA)-specific IgE Ab (E-C1) sensitized mast cells stimulated with 10 μg/mL OVA for different time periods. (d) IL-33 mRNA dose response. OVA-specific IgE Ab (E-C1) sensitized mast cells stimulated with different dose of OVA for 3 h. (e) Intracellular IL-33 expression was measured by flow cytometry for the following comparison: with or without antigen challenge and with or without antigen plus monensin. (f) Western blotting analysis of IL-33 protein expression at 8 h and 24 h following OVA challenge. Below, band intensities normalized to actin and relative to the medium (M) control group were calculated with Quantity One software (Bio-Rad). (g) IL-33 secretions from E-C1 sensitized mast cells with or without OVA stimulation detected by cell-based enzyme-linked immunosorbent assay (ELISA). Data are representative of 4 independent experiments. * P

    Journal: Journal of Interferon & Cytokine Research

    Article Title: Murine Mast Cells Secrete and Respond to Interleukin-33

    doi: 10.1089/jir.2012.0066

    Figure Lengend Snippet: Bone marrow-derived cultured mast cells (BMMCs) produce and secrete interleukin (IL)-33 through IgE-mediated activation. (a) Four-week BMMCs were stained with FcɛRI, c-kit, and CD49b, and then detected by flow cytometry. Ninety-five percent of BMMCs were FcɛRI + c-kit + CD49b − . (b) Toluidine blue staining of 4-week mast cells. (c) IL-33 mRNA kinetic expression. Ovalbumin (OVA)-specific IgE Ab (E-C1) sensitized mast cells stimulated with 10 μg/mL OVA for different time periods. (d) IL-33 mRNA dose response. OVA-specific IgE Ab (E-C1) sensitized mast cells stimulated with different dose of OVA for 3 h. (e) Intracellular IL-33 expression was measured by flow cytometry for the following comparison: with or without antigen challenge and with or without antigen plus monensin. (f) Western blotting analysis of IL-33 protein expression at 8 h and 24 h following OVA challenge. Below, band intensities normalized to actin and relative to the medium (M) control group were calculated with Quantity One software (Bio-Rad). (g) IL-33 secretions from E-C1 sensitized mast cells with or without OVA stimulation detected by cell-based enzyme-linked immunosorbent assay (ELISA). Data are representative of 4 independent experiments. * P

    Article Snippet: Bone marrow-derived cultured mast cells (BMMCs) were sensitized with 1 μg/mL of anti-ovalbumin (OVA) IgE antibody (E-C1, Chondrex INC) overnight, and then challenged with 10 μg/mL OVA (Sigma) for 8 h, with or without the addition of 2 μM monensin for the last 5 h after OVA challenge.

    Techniques: Derivative Assay, Cell Culture, Activation Assay, Staining, Flow Cytometry, Cytometry, Expressing, Western Blot, Software, Enzyme-linked Immunosorbent Assay

    Concurrent exposure to IGF2 and specific antigens induces IL-10 promoter demethylation. OVAsBCs were prepared and cultured in the presence of IGF2 and/or OVA for 72 h in the presence or absence of 5-azacytidine ( 5-aza ; a DNA methyltransferase inhibitor) or saline (control). The treatment is denoted in each panel. A and B , the immunoblots indicate the levels of IL-10 in OVAsBCs. C , IL-10 promoter methylation in OVAsBCs was assessed by methylation-specific PCR. D , the bars indicate the percentage of demethylated DNA (calculated with the data of C ). E , the immunoblots show the STAT5 gene silencing. M , methylated; U , unmethylated. shRNA STAT5 , OVAsBCs treated by STAT5 gene silencing. cshRNA , OVAsBCs treated by control shRNA. The data represent three separate experiments. Error bars represent S.D. *, p

    Journal: The Journal of Biological Chemistry

    Article Title: Insulin-like Growth Factor-2 Enhances Functions of Antigen (Ag)-specific Regulatory B Cells *

    doi: 10.1074/jbc.M113.515262

    Figure Lengend Snippet: Concurrent exposure to IGF2 and specific antigens induces IL-10 promoter demethylation. OVAsBCs were prepared and cultured in the presence of IGF2 and/or OVA for 72 h in the presence or absence of 5-azacytidine ( 5-aza ; a DNA methyltransferase inhibitor) or saline (control). The treatment is denoted in each panel. A and B , the immunoblots indicate the levels of IL-10 in OVAsBCs. C , IL-10 promoter methylation in OVAsBCs was assessed by methylation-specific PCR. D , the bars indicate the percentage of demethylated DNA (calculated with the data of C ). E , the immunoblots show the STAT5 gene silencing. M , methylated; U , unmethylated. shRNA STAT5 , OVAsBCs treated by STAT5 gene silencing. cshRNA , OVAsBCs treated by control shRNA. The data represent three separate experiments. Error bars represent S.D. *, p

    Article Snippet: 5-Azacytidine and anti-OVA antibody were purchased from Sigma-Aldrich.

    Techniques: Cell Culture, Western Blot, Methylation, Polymerase Chain Reaction, shRNA

    Induction of autoantibodies and proteinuria. BALB/c mice were repeatedly injected i.p. with 25 µg SEB, 500 µg OVA or PBS every 5 d. (A) Serum IgG- and IgM-RFs, anti-galactose-deficient IgG and anti-Sm antibodies were measured using ELISA. The arbitrary unit (AU) of 1.0 is equivalent to the titer obtained from sera of prototypic autoimmune MRL/lpr mice. Data from each mouse are connected by dotted lines. (B) Adoptive transfer of splenic B, T, CD4 + T or CD8 + T cells of SEB-, OVA- or PBS-immunized BALB/c mice into naïve BALB/c mice. The recipient mice were given single i.p. injection of 25 µg SEB or 500 µg OVA 24 h after cell transfer, and autoantibodies were measured 2 weeks later. (C) Adoptive transfer of cells from OVA-immunized BALB/c mice into β 2 m-deficient mice.

    Journal: PLoS ONE

    Article Title: Self-Organized Criticality Theory of Autoimmunity

    doi: 10.1371/journal.pone.0008382

    Figure Lengend Snippet: Induction of autoantibodies and proteinuria. BALB/c mice were repeatedly injected i.p. with 25 µg SEB, 500 µg OVA or PBS every 5 d. (A) Serum IgG- and IgM-RFs, anti-galactose-deficient IgG and anti-Sm antibodies were measured using ELISA. The arbitrary unit (AU) of 1.0 is equivalent to the titer obtained from sera of prototypic autoimmune MRL/lpr mice. Data from each mouse are connected by dotted lines. (B) Adoptive transfer of splenic B, T, CD4 + T or CD8 + T cells of SEB-, OVA- or PBS-immunized BALB/c mice into naïve BALB/c mice. The recipient mice were given single i.p. injection of 25 µg SEB or 500 µg OVA 24 h after cell transfer, and autoantibodies were measured 2 weeks later. (C) Adoptive transfer of cells from OVA-immunized BALB/c mice into β 2 m-deficient mice.

    Article Snippet: Serum IgG was quantified by ELISA (Bethyl Laboratories), and anti-OVA antibody was quantified using mouse anti-OVA monoclonal antibody (OVA-14; Sigma) as reference. (B) BALB/c mice were immunized i.p. with 100 µg KLH every 5 d. Serum RF and anti-Sm antibodies were measured by ELISA 2 d after respective immunization, AU 1.0 = equivalent detected in sera of MRL/lpr mice. (1.00 MB TIF) Click here for additional data file.

    Techniques: Mouse Assay, Injection, Enzyme-linked Immunosorbent Assay, Adoptive Transfer Assay

    Induction of autoimmune tissue injury. BALB/c mice were injected i.p. with 500 µg OVA every 5 d. (A) Serum IC measured 2 d after final immunization, expressed as AU. Proteinuria assessed 9 d after final immunization: grades 1, 2 and 3 represent 30–100 mg/dl, 100–300 mg/dl and 300–1000 mg/dl of urinary protein, respectively (upper left). Representative histopathology of kidneys from mice immunized 12× with PBS or OVA (lower left) (H E staining, bar = 20 µm; original magnification ×400): glomerular expansion with cellular infiltration including eosinophils seen under the same magnification. Immunohistochemistry for deposited IC, IgG, C3 and OVA (upper right) (bar = 50 µm; original magnification ×200), and cells infiltrated into glomeruli (bar = 20 µm; original magnification ×300), stained in serial tissue sections using anti-CD8α (53–6.7) and anti-IFNγ (R4-6A2) monoclonal antibodies, in the specimens of mice immunized 12× with OVA (lower right). (B) Lupus band test stained with anti-IgG and anti-C3 antibodies (bar = 20 µm; original magnification ×400).

    Journal: PLoS ONE

    Article Title: Self-Organized Criticality Theory of Autoimmunity

    doi: 10.1371/journal.pone.0008382

    Figure Lengend Snippet: Induction of autoimmune tissue injury. BALB/c mice were injected i.p. with 500 µg OVA every 5 d. (A) Serum IC measured 2 d after final immunization, expressed as AU. Proteinuria assessed 9 d after final immunization: grades 1, 2 and 3 represent 30–100 mg/dl, 100–300 mg/dl and 300–1000 mg/dl of urinary protein, respectively (upper left). Representative histopathology of kidneys from mice immunized 12× with PBS or OVA (lower left) (H E staining, bar = 20 µm; original magnification ×400): glomerular expansion with cellular infiltration including eosinophils seen under the same magnification. Immunohistochemistry for deposited IC, IgG, C3 and OVA (upper right) (bar = 50 µm; original magnification ×200), and cells infiltrated into glomeruli (bar = 20 µm; original magnification ×300), stained in serial tissue sections using anti-CD8α (53–6.7) and anti-IFNγ (R4-6A2) monoclonal antibodies, in the specimens of mice immunized 12× with OVA (lower right). (B) Lupus band test stained with anti-IgG and anti-C3 antibodies (bar = 20 µm; original magnification ×400).

    Article Snippet: Serum IgG was quantified by ELISA (Bethyl Laboratories), and anti-OVA antibody was quantified using mouse anti-OVA monoclonal antibody (OVA-14; Sigma) as reference. (B) BALB/c mice were immunized i.p. with 100 µg KLH every 5 d. Serum RF and anti-Sm antibodies were measured by ELISA 2 d after respective immunization, AU 1.0 = equivalent detected in sera of MRL/lpr mice. (1.00 MB TIF) Click here for additional data file.

    Techniques: Mouse Assay, Injection, Histopathology, Staining, Immunohistochemistry

    Expansion of CD8 + T cells and antigen cross-presentation. (A) Spleen cells stimulated with 50 ng/ml phorbol myristate acetate (PMA) and 500 ng/ml ionomycin for 4 h in the presence of brefeldin A (10 µg/ml) and stained for intracellular IFNγ (upper). Subsets of CD8 + T cells categorized into naïve (CD44 low CD62L high ), effector (CD44 high CD62L low ), and memory (CD44 high CD62L high ) fractions (middle). Flow cytometry of IFNγ + cells within naïve or effector/memory CD8 + T cell populations. Spleen cells were separated into naïve (CD44 low ) and effector/memory (CD44 high ) cells using CD44 MACS beads, and IFNγ + cells within the CD8 + T population was evaluated (lower). (B) Adoptive transfer of splenocytes of OVA-immunized BALB/c mice into naïve recipients. The recipients were injected with 500 µg OVA 24 h after cell transfer, and proteinuria examined 2 weeks later. (C) Cross-presentation of OVA to CD8 + T cells. Splenic CD11c + DC from OVA-immunized or control mice were incubated in the presence (OVA(+)) or absence (OVA(−)) of 1 mg/ml OVA with or without chloroquine (CQ) (20 µg/ml) for 3 h, followed by a co-culture with KJ1-26 + CD8 + T cells of DO11.10 transgenic mice for 24 h to examine surface expression of CD69 (upper). Inhibition of cross-presentation in vivo by administration of 250 µg CQ per mouse 3 h prior to immunization with OVA or PBS. IFNγ + CD8 + T cells (middle), autoantibodies and proteinuria (lower) after 12× immunization. (D) Requirement of autoantibody-inducing CD4 + T cells for CD8 + T cell-mediated autoimmune tissue injury. BALB/c mice were immunized 12× with KLH, and CD4 + T cells were isolated using MACS beads. Cells were transferred into the anti-CD4 antibody-treated recipient mice immunized 8× with OVA. Percent matured CTL, i.e., IFNγ + CD8 + T cells, and proteinuria were measured 2 weeks after booster immunization 1× with KLH.

    Journal: PLoS ONE

    Article Title: Self-Organized Criticality Theory of Autoimmunity

    doi: 10.1371/journal.pone.0008382

    Figure Lengend Snippet: Expansion of CD8 + T cells and antigen cross-presentation. (A) Spleen cells stimulated with 50 ng/ml phorbol myristate acetate (PMA) and 500 ng/ml ionomycin for 4 h in the presence of brefeldin A (10 µg/ml) and stained for intracellular IFNγ (upper). Subsets of CD8 + T cells categorized into naïve (CD44 low CD62L high ), effector (CD44 high CD62L low ), and memory (CD44 high CD62L high ) fractions (middle). Flow cytometry of IFNγ + cells within naïve or effector/memory CD8 + T cell populations. Spleen cells were separated into naïve (CD44 low ) and effector/memory (CD44 high ) cells using CD44 MACS beads, and IFNγ + cells within the CD8 + T population was evaluated (lower). (B) Adoptive transfer of splenocytes of OVA-immunized BALB/c mice into naïve recipients. The recipients were injected with 500 µg OVA 24 h after cell transfer, and proteinuria examined 2 weeks later. (C) Cross-presentation of OVA to CD8 + T cells. Splenic CD11c + DC from OVA-immunized or control mice were incubated in the presence (OVA(+)) or absence (OVA(−)) of 1 mg/ml OVA with or without chloroquine (CQ) (20 µg/ml) for 3 h, followed by a co-culture with KJ1-26 + CD8 + T cells of DO11.10 transgenic mice for 24 h to examine surface expression of CD69 (upper). Inhibition of cross-presentation in vivo by administration of 250 µg CQ per mouse 3 h prior to immunization with OVA or PBS. IFNγ + CD8 + T cells (middle), autoantibodies and proteinuria (lower) after 12× immunization. (D) Requirement of autoantibody-inducing CD4 + T cells for CD8 + T cell-mediated autoimmune tissue injury. BALB/c mice were immunized 12× with KLH, and CD4 + T cells were isolated using MACS beads. Cells were transferred into the anti-CD4 antibody-treated recipient mice immunized 8× with OVA. Percent matured CTL, i.e., IFNγ + CD8 + T cells, and proteinuria were measured 2 weeks after booster immunization 1× with KLH.

    Article Snippet: Serum IgG was quantified by ELISA (Bethyl Laboratories), and anti-OVA antibody was quantified using mouse anti-OVA monoclonal antibody (OVA-14; Sigma) as reference. (B) BALB/c mice were immunized i.p. with 100 µg KLH every 5 d. Serum RF and anti-Sm antibodies were measured by ELISA 2 d after respective immunization, AU 1.0 = equivalent detected in sera of MRL/lpr mice. (1.00 MB TIF) Click here for additional data file.

    Techniques: Staining, Flow Cytometry, Cytometry, Magnetic Cell Separation, Adoptive Transfer Assay, Mouse Assay, Injection, Incubation, Co-Culture Assay, Transgenic Assay, Expressing, Inhibition, In Vivo, Isolation, CTL Assay

    Inflammatory cell infiltrates in the lungs. Groups of wild type or B cell KO mice challenged with saline or OVA-PM were analyzed. Groups of OVA-PM challenged B cell KO mice were either controls [injected with control antibody (open circles) or given no injections (filled circles)] or injected with anti-OVA IgG1 antibody. (A) Legend. (B,C) Bar graphs show mean ± SEM and individual data for inflammatory cell infiltrates (scores, U) accumulating (B) perivascularly / peribronchially or in the (C) alveoli / interstitium of the lungs. Pairs of letters above the bars indicate the pairs of groups that showed significant differences (p

    Journal: PLoS ONE

    Article Title: The Effects of Antigen-Specific IgG1 Antibody for the Pulmonary-Hypertension-Phenotype and B Cells for Inflammation in Mice Exposed to Antigen and Fine Particles from Air Pollution

    doi: 10.1371/journal.pone.0129910

    Figure Lengend Snippet: Inflammatory cell infiltrates in the lungs. Groups of wild type or B cell KO mice challenged with saline or OVA-PM were analyzed. Groups of OVA-PM challenged B cell KO mice were either controls [injected with control antibody (open circles) or given no injections (filled circles)] or injected with anti-OVA IgG1 antibody. (A) Legend. (B,C) Bar graphs show mean ± SEM and individual data for inflammatory cell infiltrates (scores, U) accumulating (B) perivascularly / peribronchially or in the (C) alveoli / interstitium of the lungs. Pairs of letters above the bars indicate the pairs of groups that showed significant differences (p

    Article Snippet: Antibody Injections B cell KO mice were injected intraperitoneally with monoclonal mouse-anti-OVA (Sigma clone OVA-14), or mouse IgG1 isotype antibody (BioXCell) at 400 μg/dose prior to the intranasal challenges.

    Techniques: Mouse Assay, Injection

    IL-13 IL-17A response to exposure with OVA-PM 2.5 in wild type and B cell KO mice. Lymph node and lung tissues from groups of wild type or B cell KO mice challenged with saline or OVA-PM were analyzed. Groups of OVA-PM challenged B cell KO mice were either controls [injected with control antibody (open circles) or given no injections (filled circles)] or injected with anti-OVA IgG1 antibody. (A) Legend. (B) Representative dot plots were generated by flow cytometry of CD4+ T cells (CD3-CD4-dual-positive) showing staining for IL-13 vs. IL-17A. (C-E) The flow cytometry data were numerically analyzed to calculate the numbers for each cell type (mean ± SEM) per lung draining lymph node. (F-H) Gene expression in the lungs of IL-13, IL-17A, IL-17F is indicated (mean ± SEM) as fold-increase over the means of the wild type saline group. Pairs of letters above the bars indicate the pairs of groups that showed significant differences (p

    Journal: PLoS ONE

    Article Title: The Effects of Antigen-Specific IgG1 Antibody for the Pulmonary-Hypertension-Phenotype and B Cells for Inflammation in Mice Exposed to Antigen and Fine Particles from Air Pollution

    doi: 10.1371/journal.pone.0129910

    Figure Lengend Snippet: IL-13 IL-17A response to exposure with OVA-PM 2.5 in wild type and B cell KO mice. Lymph node and lung tissues from groups of wild type or B cell KO mice challenged with saline or OVA-PM were analyzed. Groups of OVA-PM challenged B cell KO mice were either controls [injected with control antibody (open circles) or given no injections (filled circles)] or injected with anti-OVA IgG1 antibody. (A) Legend. (B) Representative dot plots were generated by flow cytometry of CD4+ T cells (CD3-CD4-dual-positive) showing staining for IL-13 vs. IL-17A. (C-E) The flow cytometry data were numerically analyzed to calculate the numbers for each cell type (mean ± SEM) per lung draining lymph node. (F-H) Gene expression in the lungs of IL-13, IL-17A, IL-17F is indicated (mean ± SEM) as fold-increase over the means of the wild type saline group. Pairs of letters above the bars indicate the pairs of groups that showed significant differences (p

    Article Snippet: Antibody Injections B cell KO mice were injected intraperitoneally with monoclonal mouse-anti-OVA (Sigma clone OVA-14), or mouse IgG1 isotype antibody (BioXCell) at 400 μg/dose prior to the intranasal challenges.

    Techniques: Mouse Assay, Injection, Generated, Flow Cytometry, Cytometry, Staining, Expressing

    Right ventricular systolic pressures in wild type and B cell KO mice. Wild type and B cell KO mice were challenged with saline, or antigen and PM 2.5 (OVA-PM 2.5 ) intranasally. Data were pooled from 3 experiments; circles represent the data from individual mice, n = 7–8 per group. OVA-PM 2.5 challenged B cell KO control mice were injected with control antibody (open circles) or given no injections (filled circles). Another group of B cell KO mice was injected with antigen specific IgG1 (anti-OVA monoclonal). Right ventricular systolic pressure (RVSP, mmHg) data are shown as box plot of the medians (A) or as bar graph of the numbers of mice with RVSP less or greater than 26 mmHg (B) . The left-most beginning of each horizontal line indicates the group with which pairwise comparisons were made using the Mann-Whitney U test (A) or Fisher’s exact test (B) . Significant P values (P

    Journal: PLoS ONE

    Article Title: The Effects of Antigen-Specific IgG1 Antibody for the Pulmonary-Hypertension-Phenotype and B Cells for Inflammation in Mice Exposed to Antigen and Fine Particles from Air Pollution

    doi: 10.1371/journal.pone.0129910

    Figure Lengend Snippet: Right ventricular systolic pressures in wild type and B cell KO mice. Wild type and B cell KO mice were challenged with saline, or antigen and PM 2.5 (OVA-PM 2.5 ) intranasally. Data were pooled from 3 experiments; circles represent the data from individual mice, n = 7–8 per group. OVA-PM 2.5 challenged B cell KO control mice were injected with control antibody (open circles) or given no injections (filled circles). Another group of B cell KO mice was injected with antigen specific IgG1 (anti-OVA monoclonal). Right ventricular systolic pressure (RVSP, mmHg) data are shown as box plot of the medians (A) or as bar graph of the numbers of mice with RVSP less or greater than 26 mmHg (B) . The left-most beginning of each horizontal line indicates the group with which pairwise comparisons were made using the Mann-Whitney U test (A) or Fisher’s exact test (B) . Significant P values (P

    Article Snippet: Antibody Injections B cell KO mice were injected intraperitoneally with monoclonal mouse-anti-OVA (Sigma clone OVA-14), or mouse IgG1 isotype antibody (BioXCell) at 400 μg/dose prior to the intranasal challenges.

    Techniques: Mouse Assay, Injection, MANN-WHITNEY

    Markers of Th17 inflammation in the lungs. Groups of wild type or B cell KO mice challenged with saline or OVA-PM were analyzed. Groups of OVA-PM challenged B cell KO mice were either controls [injected with control antibody (open circles) or given no injections (filled circles)] or injected with anti-OVA IgG1 antibody. (A) Legend. (B-E) Bar graphs show mean ± SEM and individual data points for (B) numbers of BAL neutrophils; (C) S100a8; (D) S100a9; and (E) IL-6 gene expression. Gene expression in the lungs is shown as fold-increase over the means of the wild type saline group. Pairs of letters above the bars indicate the pairs of groups that showed significant differences (p

    Journal: PLoS ONE

    Article Title: The Effects of Antigen-Specific IgG1 Antibody for the Pulmonary-Hypertension-Phenotype and B Cells for Inflammation in Mice Exposed to Antigen and Fine Particles from Air Pollution

    doi: 10.1371/journal.pone.0129910

    Figure Lengend Snippet: Markers of Th17 inflammation in the lungs. Groups of wild type or B cell KO mice challenged with saline or OVA-PM were analyzed. Groups of OVA-PM challenged B cell KO mice were either controls [injected with control antibody (open circles) or given no injections (filled circles)] or injected with anti-OVA IgG1 antibody. (A) Legend. (B-E) Bar graphs show mean ± SEM and individual data points for (B) numbers of BAL neutrophils; (C) S100a8; (D) S100a9; and (E) IL-6 gene expression. Gene expression in the lungs is shown as fold-increase over the means of the wild type saline group. Pairs of letters above the bars indicate the pairs of groups that showed significant differences (p

    Article Snippet: Antibody Injections B cell KO mice were injected intraperitoneally with monoclonal mouse-anti-OVA (Sigma clone OVA-14), or mouse IgG1 isotype antibody (BioXCell) at 400 μg/dose prior to the intranasal challenges.

    Techniques: Mouse Assay, Injection, Expressing

    Right ventricular (RV) weight and RV-gene expression. Groups of wild type and B cell KO mice challenged with saline or OVA-PM were analyzed. Groups of OVA-PM challenged B cell KO mice were either controls [injected with control antibody (open circles) or given no injections (filled circles)] or injected with anti-OVA IgG1 antibody. (A) Legend. (B-G) Bar graphs show mean ± SEM and individual data for right ventricular weight calculated relative to (B) the weight of the left ventricle and septum (RV/LV+S), or (C) body weight (RV/BW); and gene expression in the right ventricle of [BNP (D) , IL-33 (E) , RELMα (F) , RELMγ (G) ]. Gene expression in the lungs is shown as fold-increase over the means of the wild type saline group. Pairs of letters above the bars indicate the pairs of groups that showed significant differences (p

    Journal: PLoS ONE

    Article Title: The Effects of Antigen-Specific IgG1 Antibody for the Pulmonary-Hypertension-Phenotype and B Cells for Inflammation in Mice Exposed to Antigen and Fine Particles from Air Pollution

    doi: 10.1371/journal.pone.0129910

    Figure Lengend Snippet: Right ventricular (RV) weight and RV-gene expression. Groups of wild type and B cell KO mice challenged with saline or OVA-PM were analyzed. Groups of OVA-PM challenged B cell KO mice were either controls [injected with control antibody (open circles) or given no injections (filled circles)] or injected with anti-OVA IgG1 antibody. (A) Legend. (B-G) Bar graphs show mean ± SEM and individual data for right ventricular weight calculated relative to (B) the weight of the left ventricle and septum (RV/LV+S), or (C) body weight (RV/BW); and gene expression in the right ventricle of [BNP (D) , IL-33 (E) , RELMα (F) , RELMγ (G) ]. Gene expression in the lungs is shown as fold-increase over the means of the wild type saline group. Pairs of letters above the bars indicate the pairs of groups that showed significant differences (p

    Article Snippet: Antibody Injections B cell KO mice were injected intraperitoneally with monoclonal mouse-anti-OVA (Sigma clone OVA-14), or mouse IgG1 isotype antibody (BioXCell) at 400 μg/dose prior to the intranasal challenges.

    Techniques: Expressing, Mouse Assay, Injection

    OVA-specific IgG1 serum levels in sensitized wild type mice and in B cell KO mice following reconstitution with monoclonal anti-OVA IgG1 antibody. OVA-specific IgG1 levels were measured in sera of sensitized wild type mice that were either challenged intranasally with saline or OVA-PM, and in sera of sensitized B cell KO mice that were either controls or injected with anti-OVA IgG1 antibody. (A) Correlation of IgG1 serum titers with right ventricular systolic pressures in sensitized wild type mice. Values for P and rs (tie corrected, Spearman’s rank correlation test) are indicated. (B) Bar graphs show (mean ± SEM) and individual data points of OVA-specific IgG1 levels. Pairs of letters above the bars indicate the pairs of groups that showed significant differences (p

    Journal: PLoS ONE

    Article Title: The Effects of Antigen-Specific IgG1 Antibody for the Pulmonary-Hypertension-Phenotype and B Cells for Inflammation in Mice Exposed to Antigen and Fine Particles from Air Pollution

    doi: 10.1371/journal.pone.0129910

    Figure Lengend Snippet: OVA-specific IgG1 serum levels in sensitized wild type mice and in B cell KO mice following reconstitution with monoclonal anti-OVA IgG1 antibody. OVA-specific IgG1 levels were measured in sera of sensitized wild type mice that were either challenged intranasally with saline or OVA-PM, and in sera of sensitized B cell KO mice that were either controls or injected with anti-OVA IgG1 antibody. (A) Correlation of IgG1 serum titers with right ventricular systolic pressures in sensitized wild type mice. Values for P and rs (tie corrected, Spearman’s rank correlation test) are indicated. (B) Bar graphs show (mean ± SEM) and individual data points of OVA-specific IgG1 levels. Pairs of letters above the bars indicate the pairs of groups that showed significant differences (p

    Article Snippet: Antibody Injections B cell KO mice were injected intraperitoneally with monoclonal mouse-anti-OVA (Sigma clone OVA-14), or mouse IgG1 isotype antibody (BioXCell) at 400 μg/dose prior to the intranasal challenges.

    Techniques: Mouse Assay, Injection

    Markers of T (helper)h2 inflammation in the lungs. Groups of wild type or B cell KO mice challenged with saline or OVA-PM were analyzed. Groups of OVA-PM challenged B cell KO mice were either controls [injected with control antibody (open circles) or given no injections (filled circles)] or injected with anti-OVA IgG1 antibody. (A) Legend. (B-F) Bar graphs show mean ± SEM and individual data points for (B) numbers of bronchoalveolar lavage (BAL) eosinophils; (C) mean fluorescent intensity (MFI) of major histocompatibility complex class II (MHCII) of BAL CD11c+ cells; (D) RELMβ; (E) IL-33; and (F) miR-135a expression in the lungs. Gene expression in the lungs is shown as fold-increase over the means of the wild type saline group. Pairs of letters above the bars indicate the pairs of groups that showed significant differences (p

    Journal: PLoS ONE

    Article Title: The Effects of Antigen-Specific IgG1 Antibody for the Pulmonary-Hypertension-Phenotype and B Cells for Inflammation in Mice Exposed to Antigen and Fine Particles from Air Pollution

    doi: 10.1371/journal.pone.0129910

    Figure Lengend Snippet: Markers of T (helper)h2 inflammation in the lungs. Groups of wild type or B cell KO mice challenged with saline or OVA-PM were analyzed. Groups of OVA-PM challenged B cell KO mice were either controls [injected with control antibody (open circles) or given no injections (filled circles)] or injected with anti-OVA IgG1 antibody. (A) Legend. (B-F) Bar graphs show mean ± SEM and individual data points for (B) numbers of bronchoalveolar lavage (BAL) eosinophils; (C) mean fluorescent intensity (MFI) of major histocompatibility complex class II (MHCII) of BAL CD11c+ cells; (D) RELMβ; (E) IL-33; and (F) miR-135a expression in the lungs. Gene expression in the lungs is shown as fold-increase over the means of the wild type saline group. Pairs of letters above the bars indicate the pairs of groups that showed significant differences (p

    Article Snippet: Antibody Injections B cell KO mice were injected intraperitoneally with monoclonal mouse-anti-OVA (Sigma clone OVA-14), or mouse IgG1 isotype antibody (BioXCell) at 400 μg/dose prior to the intranasal challenges.

    Techniques: Mouse Assay, Injection, Expressing

    Severe thickening of pulmonary arteries and expression of pro-remodeling genes in the lungs. Groups of wild type or B cell KO mice challenged with saline or OVA-PM were analyzed. Groups of OVA-PM challenged B cell KO mice were either controls [injected with control antibody (open circles) or given no injections (filled circles)] or injected with anti-OVA IgG1 antibody. (A) Legend. (B-D) Bar graphs show mean ± SEM and individual data for (B) severe arterial thickening by histological analysis and for expression of pro-remodeling genes ( C RELMα, D MMP12) in the lungs. Gene expression in the lungs is shown as fold-increase over the means of the wild type saline group. Pairs of letters above the bars indicate the pairs of groups that showed significant differences (p

    Journal: PLoS ONE

    Article Title: The Effects of Antigen-Specific IgG1 Antibody for the Pulmonary-Hypertension-Phenotype and B Cells for Inflammation in Mice Exposed to Antigen and Fine Particles from Air Pollution

    doi: 10.1371/journal.pone.0129910

    Figure Lengend Snippet: Severe thickening of pulmonary arteries and expression of pro-remodeling genes in the lungs. Groups of wild type or B cell KO mice challenged with saline or OVA-PM were analyzed. Groups of OVA-PM challenged B cell KO mice were either controls [injected with control antibody (open circles) or given no injections (filled circles)] or injected with anti-OVA IgG1 antibody. (A) Legend. (B-D) Bar graphs show mean ± SEM and individual data for (B) severe arterial thickening by histological analysis and for expression of pro-remodeling genes ( C RELMα, D MMP12) in the lungs. Gene expression in the lungs is shown as fold-increase over the means of the wild type saline group. Pairs of letters above the bars indicate the pairs of groups that showed significant differences (p

    Article Snippet: Antibody Injections B cell KO mice were injected intraperitoneally with monoclonal mouse-anti-OVA (Sigma clone OVA-14), or mouse IgG1 isotype antibody (BioXCell) at 400 μg/dose prior to the intranasal challenges.

    Techniques: Expressing, Mouse Assay, Injection

    Induction of autoantibodies and proteinuria. BALB/c mice were repeatedly injected i.p. with 25 µg SEB, 500 µg OVA or PBS every 5 d. (A) Serum IgG- and IgM-RFs, anti-galactose-deficient IgG and anti-Sm antibodies were measured using ELISA. The arbitrary unit (AU) of 1.0 is equivalent to the titer obtained from sera of prototypic autoimmune MRL/lpr mice. Data from each mouse are connected by dotted lines. (B) Adoptive transfer of splenic B, T, CD4 + T or CD8 + T cells of SEB-, OVA- or PBS-immunized BALB/c mice into naïve BALB/c mice. The recipient mice were given single i.p. injection of 25 µg SEB or 500 µg OVA 24 h after cell transfer, and autoantibodies were measured 2 weeks later. (C) Adoptive transfer of cells from OVA-immunized BALB/c mice into β 2 m-deficient mice.

    Journal: PLoS ONE

    Article Title: Self-Organized Criticality Theory of Autoimmunity

    doi: 10.1371/journal.pone.0008382

    Figure Lengend Snippet: Induction of autoantibodies and proteinuria. BALB/c mice were repeatedly injected i.p. with 25 µg SEB, 500 µg OVA or PBS every 5 d. (A) Serum IgG- and IgM-RFs, anti-galactose-deficient IgG and anti-Sm antibodies were measured using ELISA. The arbitrary unit (AU) of 1.0 is equivalent to the titer obtained from sera of prototypic autoimmune MRL/lpr mice. Data from each mouse are connected by dotted lines. (B) Adoptive transfer of splenic B, T, CD4 + T or CD8 + T cells of SEB-, OVA- or PBS-immunized BALB/c mice into naïve BALB/c mice. The recipient mice were given single i.p. injection of 25 µg SEB or 500 µg OVA 24 h after cell transfer, and autoantibodies were measured 2 weeks later. (C) Adoptive transfer of cells from OVA-immunized BALB/c mice into β 2 m-deficient mice.

    Article Snippet: Mice (8 weeks-old) were immunized with 25 µg SEB (Toxin Technologies, Sarasota, FL), 500 µg OVA (grade V; Sigma, St. Louis, MO), 100 µg KLH (Sigma) or PBS by means of i.p. injection every 5 d. Frozen sections of kidney and dermis were stained for C3, IgG or OVA using goat anti-C3 (Bethyl laboratories, Inc., Montgomery, TX) and Alexa Fluor 488-conjugated anti-goat IgG antibodies (Molecular Probes, Eugene, OR), Alexa Fluor 594-conjugated anti-mouse IgG antibody (Molecular Probes), or rabbit anti-OVA antibody (Sigma).

    Techniques: Mouse Assay, Injection, Enzyme-linked Immunosorbent Assay, Adoptive Transfer Assay

    Induction of autoimmune tissue injury. BALB/c mice were injected i.p. with 500 µg OVA every 5 d. (A) Serum IC measured 2 d after final immunization, expressed as AU. Proteinuria assessed 9 d after final immunization: grades 1, 2 and 3 represent 30–100 mg/dl, 100–300 mg/dl and 300–1000 mg/dl of urinary protein, respectively (upper left). Representative histopathology of kidneys from mice immunized 12× with PBS or OVA (lower left) (H E staining, bar = 20 µm; original magnification ×400): glomerular expansion with cellular infiltration including eosinophils seen under the same magnification. Immunohistochemistry for deposited IC, IgG, C3 and OVA (upper right) (bar = 50 µm; original magnification ×200), and cells infiltrated into glomeruli (bar = 20 µm; original magnification ×300), stained in serial tissue sections using anti-CD8α (53–6.7) and anti-IFNγ (R4-6A2) monoclonal antibodies, in the specimens of mice immunized 12× with OVA (lower right). (B) Lupus band test stained with anti-IgG and anti-C3 antibodies (bar = 20 µm; original magnification ×400).

    Journal: PLoS ONE

    Article Title: Self-Organized Criticality Theory of Autoimmunity

    doi: 10.1371/journal.pone.0008382

    Figure Lengend Snippet: Induction of autoimmune tissue injury. BALB/c mice were injected i.p. with 500 µg OVA every 5 d. (A) Serum IC measured 2 d after final immunization, expressed as AU. Proteinuria assessed 9 d after final immunization: grades 1, 2 and 3 represent 30–100 mg/dl, 100–300 mg/dl and 300–1000 mg/dl of urinary protein, respectively (upper left). Representative histopathology of kidneys from mice immunized 12× with PBS or OVA (lower left) (H E staining, bar = 20 µm; original magnification ×400): glomerular expansion with cellular infiltration including eosinophils seen under the same magnification. Immunohistochemistry for deposited IC, IgG, C3 and OVA (upper right) (bar = 50 µm; original magnification ×200), and cells infiltrated into glomeruli (bar = 20 µm; original magnification ×300), stained in serial tissue sections using anti-CD8α (53–6.7) and anti-IFNγ (R4-6A2) monoclonal antibodies, in the specimens of mice immunized 12× with OVA (lower right). (B) Lupus band test stained with anti-IgG and anti-C3 antibodies (bar = 20 µm; original magnification ×400).

    Article Snippet: Mice (8 weeks-old) were immunized with 25 µg SEB (Toxin Technologies, Sarasota, FL), 500 µg OVA (grade V; Sigma, St. Louis, MO), 100 µg KLH (Sigma) or PBS by means of i.p. injection every 5 d. Frozen sections of kidney and dermis were stained for C3, IgG or OVA using goat anti-C3 (Bethyl laboratories, Inc., Montgomery, TX) and Alexa Fluor 488-conjugated anti-goat IgG antibodies (Molecular Probes, Eugene, OR), Alexa Fluor 594-conjugated anti-mouse IgG antibody (Molecular Probes), or rabbit anti-OVA antibody (Sigma).

    Techniques: Mouse Assay, Injection, Histopathology, Staining, Immunohistochemistry

    Cyclic peptide CsA mediated inhibition of OVA amyloid synthesis as revealed by fluoresense microscopy. Green fluorescence associated with OVA amyloid bound ThT was visualized employing epifluoresense microscopy. The epi-fluoprescence micrographs of the as-synthesized OVA amyloid formed in absenccse ( A ) or presense ( B ) of 500 nM CsA. The panel (C) corresponds to Western blot profile of amyloid intermediates formed during fibril formation. Western blot analysis of OVA-amyloid probed with OVA specific 3G2E1D9 monoclonal antibodies reveals presence of monomeric, dimeric as well as oligomeric OVA (lane i) formed at the beginning of exponential phase of OVA aggregation process (without CsA). Lane ii shows inhibitory effect CsA (500 nM) on the formation of monomeric and dimeric intermediates of the OVA.

    Journal: Scientific Reports

    Article Title: Cyclic undecapeptide Cyclosporin A mediated inhibition of amyloid synthesis: Implications in alleviation of amyloid induced neurotoxicity

    doi: 10.1038/s41598-018-35645-4

    Figure Lengend Snippet: Cyclic peptide CsA mediated inhibition of OVA amyloid synthesis as revealed by fluoresense microscopy. Green fluorescence associated with OVA amyloid bound ThT was visualized employing epifluoresense microscopy. The epi-fluoprescence micrographs of the as-synthesized OVA amyloid formed in absenccse ( A ) or presense ( B ) of 500 nM CsA. The panel (C) corresponds to Western blot profile of amyloid intermediates formed during fibril formation. Western blot analysis of OVA-amyloid probed with OVA specific 3G2E1D9 monoclonal antibodies reveals presence of monomeric, dimeric as well as oligomeric OVA (lane i) formed at the beginning of exponential phase of OVA aggregation process (without CsA). Lane ii shows inhibitory effect CsA (500 nM) on the formation of monomeric and dimeric intermediates of the OVA.

    Article Snippet: Anti-OVA monoclonal antibody 3G2E1D9 was purchased from Santa Cruz Biotechnology, Inc.3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) was obtained from Amresco,USA.

    Techniques: Inhibition, Microscopy, Fluorescence, Synthesized, Western Blot