Journal: Journal of Biological Chemistry

Article Title: Taste of a Pill

doi: 10.1074/jbc.m114.570564

Figure Lengend Snippet: FIGURE 4. Localization of OCT3/Oct3 in salivary gland epithelial cells. A, detection of OCT3 (panel i, green) in human submandibular glands. Staining of Na/K-ATPase (panel ii), a basolateral marker, and nuclei (panel iii) is shown in red and blue, respectively. B, detection of Oct3 (panel i, green) and nuclei (panel ii, blue) in salivary gland sections from Oct3/ (upper panels) and Oct3/ (lower panels) mice. In overlays (A, panel iv, and B, panel iii), the arrow and arrowhead indicate basolateral and apical membranes of salivary gland epithelial cells, respectively.

Article Snippet: The sections were blocked in goat serum in PBS, incu- bated overnight at 4 °C with rabbit anti-OCT3 polyclonal antibody (1:125 dilution; Genway), and co-labeled with anti-human Na /K -ATPase -subunit monoclonal antibody (1:500 dilution; Sigma).

Techniques: Staining, Marker

Journal: Journal of Biological Chemistry

Article Title: Taste of a Pill

doi: 10.1074/jbc.m114.570564

Figure Lengend Snippet: FIGURE 7. Model proposed for OCT3-mediated organic cation (OC) transport in salivary gland epithelial cells. OCT3 on the basolateral membrane of epithelial cells mediates metformin uptake from the blood into the cells. Once metformin is highly concentrated inside the cells, OCT3 on the apical membrane facilitateseffluxofmetforminintothesaliva.Thesolidarrowsindicatethepreferreddirectionofmetformintransportwhendrugconcentrationsinthesystemic circulation are high. The dashed arrows indicate the possible transport direction when systemic drug concentrations decline.

Article Snippet: The sections were blocked in goat serum in PBS, incu- bated overnight at 4 °C with rabbit anti-OCT3 polyclonal antibody (1:125 dilution; Genway), and co-labeled with anti-human Na /K -ATPase -subunit monoclonal antibody (1:500 dilution; Sigma).

Techniques: Membrane

Journal: Molecular Medicine Reports

Article Title: Knockdown of OCT4 suppresses the growth and invasion of pancreatic cancer cells through inhibition of the AKT pathway

doi: 10.3892/mmr.2014.2367

Figure Lengend Snippet: Expression of OCT4 protein in pancreatic cancer tissues (magnification, ×200). Pancreatic cancer tissues and ANCT were immunohistochemically stained with an anti-OCT4 antibody and classified as (−) and (+). (A) Positive expression in pancreatic cancer. (B) Negative expression in pancreatic cancer. (C) Positive expression in ANCT. (D) Negative expression in ANCT. Positive immunostaining of OCT4 was mainly localized in the nucleus of the tumor and ANCT cells. OCT4, octamer-binding transcription factor 4; ANCT, adjacent non-cancer tissues.

Article Snippet: Normal serum or phosphate-buffered saline (PBS; Wuhan Boster Biological Engineering Co., Ltd.) was used to replace anti-OCT4 antibody in the negative controls.

Techniques: Expressing, Staining, Immunostaining, Binding Assay

Journal: Molecular Medicine Reports

Article Title: Knockdown of OCT4 suppresses the growth and invasion of pancreatic cancer cells through inhibition of the AKT pathway

doi: 10.3892/mmr.2014.2367

Figure Lengend Snippet: Expression of OCT4 protein in pancreatic cancer tissues.

Article Snippet: Normal serum or phosphate-buffered saline (PBS; Wuhan Boster Biological Engineering Co., Ltd.) was used to replace anti-OCT4 antibody in the negative controls.

Techniques: Expressing

Journal: Molecular Medicine Reports

Article Title: Knockdown of OCT4 suppresses the growth and invasion of pancreatic cancer cells through inhibition of the AKT pathway

doi: 10.3892/mmr.2014.2367

Figure Lengend Snippet: Expression of OCT4 in pancreatic cancer cells with different degrees of differentiation. The expression of OCT4 in pancreatic cancer cells with different degrees of differentiation (Bxpc3, Panc-1 and Mia PaCa-2) was examined by (A) real-time PCR and (B and C) western blot assays, of which OCT4 was highly expressed in the Panc-1 cell line compared with the other two cell lines (P<0.01). OCT4, octamer binding transcription factor 4.

Article Snippet: Normal serum or phosphate-buffered saline (PBS; Wuhan Boster Biological Engineering Co., Ltd.) was used to replace anti-OCT4 antibody in the negative controls.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Binding Assay

Journal: Molecular Medicine Reports

Article Title: Knockdown of OCT4 suppresses the growth and invasion of pancreatic cancer cells through inhibition of the AKT pathway

doi: 10.3892/mmr.2014.2367

Figure Lengend Snippet: Correlation of OCT4 expression with the clinicopathological characteristics of patients with pancreatic cancer.

Article Snippet: Normal serum or phosphate-buffered saline (PBS; Wuhan Boster Biological Engineering Co., Ltd.) was used to replace anti-OCT4 antibody in the negative controls.

Techniques: Expressing

Journal: Molecular Medicine Reports

Article Title: Knockdown of OCT4 suppresses the growth and invasion of pancreatic cancer cells through inhibition of the AKT pathway

doi: 10.3892/mmr.2014.2367

Figure Lengend Snippet: Effect of OCT4 knockdown on the expression of AKT in pancreatic cancer cells. After pancreatic cancer cells were transfected with the Lv-shOCT4 for 24 h, the expression levels of OCT4 and AKT were detected by (A and B) real-time PCR and (C–F) western blot analysis. The expression of OCT4 and AKT was significantly decreased in the Lv-shOCT4 group compared with the CON and NC groups ( ** P<0.01). OCT4, octamer binding transcription factor 4; Lv-shOCT4, lentivirus-mediated OCT4 shRNA vector; CON, control vector; NC, negative control vector.

Article Snippet: Normal serum or phosphate-buffered saline (PBS; Wuhan Boster Biological Engineering Co., Ltd.) was used to replace anti-OCT4 antibody in the negative controls.

Techniques: Expressing, Transfection, Real-time Polymerase Chain Reaction, Western Blot, Binding Assay, shRNA, Plasmid Preparation, Negative Control

Journal: Molecular Medicine Reports

Article Title: Knockdown of OCT4 suppresses the growth and invasion of pancreatic cancer cells through inhibition of the AKT pathway

doi: 10.3892/mmr.2014.2367

Figure Lengend Snippet: Effect of OCT4 knockdown on cell proliferation. (A) Cell proliferative activity, indicated by MTT assay, markedly decreased in a time-dependent manner in the Lv-shOCT4 group compared with the CON and NC groups ( ** P<0.01). (B and C) Endogenous expression of PCNA, indicated by western blot analysis, was significantly decreased in the Lv-shOCT4 group compared with the NC and CON groups ( ** P<0.01). OCT4, octamer binding transcription factor 4; Lv-shOCT4, lentivirus-mediated OCT4 shRNA vector; PCNA, proliferating cell nuclear antigen; CON, control vector; NC, negative control vector.

Article Snippet: Normal serum or phosphate-buffered saline (PBS; Wuhan Boster Biological Engineering Co., Ltd.) was used to replace anti-OCT4 antibody in the negative controls.

Techniques: Activity Assay, MTT Assay, Expressing, Western Blot, Binding Assay, shRNA, Plasmid Preparation, Negative Control

Journal: Molecular Medicine Reports

Article Title: Knockdown of OCT4 suppresses the growth and invasion of pancreatic cancer cells through inhibition of the AKT pathway

doi: 10.3892/mmr.2014.2367

Figure Lengend Snippet: Effect of OCT4 knockdown on cell invasion (magnification, ×200). (A and B) Cell invasive potential, indicated by Transwell assay, was markedly weakened in the Lv-shOCT4 group compared with the CON and NC groups ( ** P<0.01). (C and D) Endogenous expression of MMP-2, indicated by western blot analysis, was significantly decreased in the Lv-shOCT4 group compared with the NC and CON groups ( ** P<0.01). OCT4, octamer binding transcription factor 4; Lv-shOCT4, lentivirus-mediated OCT4 shRNA vector; CON, control vector; NC, negative control vector; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MMP-2, matrix metalloproteinase-2.

Article Snippet: Normal serum or phosphate-buffered saline (PBS; Wuhan Boster Biological Engineering Co., Ltd.) was used to replace anti-OCT4 antibody in the negative controls.

Techniques: Transwell Assay, Expressing, Western Blot, Binding Assay, shRNA, Plasmid Preparation, Negative Control

Journal: Oncotarget

Article Title: Graded expression of microRNA-371a-3p in tumor tissues, contralateral testes, and in serum of patients with testicular germ cell tumor

doi: 10.18632/oncotarget.27565

Figure Lengend Snippet: ( A ) In situ hybridization with a probe against miR-371a-3p causes blue staining in cells. ( B ) Section from A. ( C ) Immunohistochemical staining of the same area with an OCT4 antibody for identification of EC cells. ( D ) H&E staining of the same area.

Article Snippet: OCT4 staining for identification of embryonal carcinomas (EC), PLAP staining for seminomas (SE) and glypican 3 for yolk-sac tumors (YST) (Diagnostic BioSystems, Pleasanton, CA, USA) were then conducted according to institutional standard operating procedures [ , ].

Techniques: In Situ Hybridization, Staining, Immunohistochemical staining