Journal: Nutrition & Metabolism
Article Title: An excess dietary vitamin E concentration does not influence Nrf2 signaling in the liver of rats fed either soybean oil or salmon oil
Figure Lengend Snippet: Effect on protein expression of Nrf2 targets. Relative Protein expression of GPX ( a ), HO-1 ( b ) and NQO1 ( c ) in the liver of rats fed diets with either soybean oil or salmon oil with various vitamin E concentrations. Bars represent relative protein level expressed as fold of control (diet with soybean oil and 25 mg/kg vitamin E) and are means ± SD from 12 animals per group. Representative immunoblots specific to GPX ( a ), HO-1 ( b ), NQO1 ( c ) and ß-actin as respective loading control are shown for one animal per group
Article Snippet: After that, the membranes were washed and blocked for 1 h at room temperature with 5% nonfat dry milk in TBS-T (w /v ) following incubations with primary antibodies against BIP (rabbit polyclonal anti-GRP78 antibody, Thermo Fisher Scientific Cat# PA5–29705, RRID:AB_2547179, Schwerte, Germany), p-eIF2α (rabbit polyclonal anti-pSer51 antibody, Cell Signaling Technology Cat# 9721, RRID:AB_330951, Frankfurt/Main, Germany), eIF2α (rabbit polyclonal anti-eIF2α antibody, Cell Signaling Technology Cat# 9722, RRID:AB_2230924), GPX (rabbit polyclonal anti-GPX antibody, Abcam Cat# ab22604, RRID:AB_2112120, Cambridge, UK), HO1 (rabbit polyclonal anti-HO1; Abcam Cat# ab68477, RRID:AB_11156457), Abcam), NQO1 (goat polyclonal, Santa Cruz Biotechnology Cat# sc-16,464, RRID:AB_2154339, Heidelberg, Germany) and β-actin (mouse monoclonal anti-β-actin, Abcam Cat# ab6276, RRID:AB_2223210, Abcam) or α-tubulin (rabbit monoclonal anti-α-tubulin, Cell Signaling Technology Cat# 2125, RRID:AB_2619646) as a reference protein for adequate normalization.
Techniques: Expressing, Western Blot