Journal: Current Alzheimer Research

Article Title: Anti-α4β1 Integrin Antibodies Attenuated Brain Inflammatory Changes in a Mouse Model of Alzheimer’s Disease

doi: 10.2174/1567205015666180801111033

Figure Lengend Snippet: Both the isotype control and anti-CD49d antibody injections affected brain CD4 immunoreactivity. C57BL/6 and APP/PS1 mice were injected intravenously (tail-vein) with Saline, IgG isotype control (purified NA/LE Rat IgG2b) (I.C.) or 2 mg/kg purified NA/LE rat anti-mouse CD49d (anti-CD49d) once a week for 4 weeks. Right brain hemispheres from all the mice were fixed and serially sectioned. The presence of IgG2b antibody in the brain was assessed by immunostaining using biotinylated anti-mouse IgG2b antibody (8A) . The presence of T cells in the brain was visualized using anti-CD4 antibody and immunoreactivity observed in the striatum (8B) and parietal cortex (8C) was imaged. Representative images from 3-6 animals per group are shown at 20X magnification with 63X magnification insets.

Article Snippet: Serial sections (960µm apart) were immunostained using anti-Aβ (4G8), anti-TMEM119 and anti-GFAP antibodies at a dilution of 1:1000 or anti-CD68, anti-CD4 and anti-Iba-1antibodies at a dilution of 1:500 followed by incubation with biotinylated secondary antibodies (1:2000 dilution) (Vector Laboratories Inc., Burlingame, CA) and avidin/biotin solution (Vector ABC kit).

Techniques: Mouse Assay, Injection, Purification, Immunostaining