anti-mouse secondary antibodies Search Results


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  • 95
    Thermo Fisher antimouse secondary antibodies
    Phospho-JNK localization during VACV infection. BSC-40 cells were transfected with pcDNA3-cMyc-JNK2-MKK7 for 48 h, followed by infection with VACV for 7 h. At this time, cells were processed for fluorescence analyses and simultaneously stained with anti-c-Myc and antiviral protein I3 antibodies, followed by FITC-conjugated <t>antimouse</t> and rhodamine-conjugated antirabbit antibodies (green and red, respectively). To detect cellular and viral DNA, cells were stained with DAPI (blue).
    Antimouse Secondary Antibodies, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher goat antimouse secondary antibody
    Phospho-JNK localization during VACV infection. BSC-40 cells were transfected with pcDNA3-cMyc-JNK2-MKK7 for 48 h, followed by infection with VACV for 7 h. At this time, cells were processed for fluorescence analyses and simultaneously stained with anti-c-Myc and antiviral protein I3 antibodies, followed by FITC-conjugated <t>antimouse</t> and rhodamine-conjugated antirabbit antibodies (green and red, respectively). To detect cellular and viral DNA, cells were stained with DAPI (blue).
    Goat Antimouse Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher antimouse igg secondary antibodies
    Phospho-JNK localization during VACV infection. BSC-40 cells were transfected with pcDNA3-cMyc-JNK2-MKK7 for 48 h, followed by infection with VACV for 7 h. At this time, cells were processed for fluorescence analyses and simultaneously stained with anti-c-Myc and antiviral protein I3 antibodies, followed by FITC-conjugated <t>antimouse</t> and rhodamine-conjugated antirabbit antibodies (green and red, respectively). To detect cellular and viral DNA, cells were stained with DAPI (blue).
    Antimouse Igg Secondary Antibodies, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher rabbit antimouse igg secondary antibody
    Phospho-JNK localization during VACV infection. BSC-40 cells were transfected with pcDNA3-cMyc-JNK2-MKK7 for 48 h, followed by infection with VACV for 7 h. At this time, cells were processed for fluorescence analyses and simultaneously stained with anti-c-Myc and antiviral protein I3 antibodies, followed by FITC-conjugated <t>antimouse</t> and rhodamine-conjugated antirabbit antibodies (green and red, respectively). To detect cellular and viral DNA, cells were stained with DAPI (blue).
    Rabbit Antimouse Igg Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher antimouse secondary antibodies conjugated
    Phospho-JNK localization during VACV infection. BSC-40 cells were transfected with pcDNA3-cMyc-JNK2-MKK7 for 48 h, followed by infection with VACV for 7 h. At this time, cells were processed for fluorescence analyses and simultaneously stained with anti-c-Myc and antiviral protein I3 antibodies, followed by FITC-conjugated <t>antimouse</t> and rhodamine-conjugated antirabbit antibodies (green and red, respectively). To detect cellular and viral DNA, cells were stained with DAPI (blue).
    Antimouse Secondary Antibodies Conjugated, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher antimouse igg antibodies
    Phospho-JNK localization during VACV infection. BSC-40 cells were transfected with pcDNA3-cMyc-JNK2-MKK7 for 48 h, followed by infection with VACV for 7 h. At this time, cells were processed for fluorescence analyses and simultaneously stained with anti-c-Myc and antiviral protein I3 antibodies, followed by FITC-conjugated <t>antimouse</t> and rhodamine-conjugated antirabbit antibodies (green and red, respectively). To detect cellular and viral DNA, cells were stained with DAPI (blue).
    Antimouse Igg Antibodies, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher rabbit antimouse secondary antibodies
    Phospho-JNK localization during VACV infection. BSC-40 cells were transfected with pcDNA3-cMyc-JNK2-MKK7 for 48 h, followed by infection with VACV for 7 h. At this time, cells were processed for fluorescence analyses and simultaneously stained with anti-c-Myc and antiviral protein I3 antibodies, followed by FITC-conjugated <t>antimouse</t> and rhodamine-conjugated antirabbit antibodies (green and red, respectively). To detect cellular and viral DNA, cells were stained with DAPI (blue).
    Rabbit Antimouse Secondary Antibodies, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Thermo Fisher secondary antimouse antibody
    Phospho-JNK localization during VACV infection. BSC-40 cells were transfected with pcDNA3-cMyc-JNK2-MKK7 for 48 h, followed by infection with VACV for 7 h. At this time, cells were processed for fluorescence analyses and simultaneously stained with anti-c-Myc and antiviral protein I3 antibodies, followed by FITC-conjugated <t>antimouse</t> and rhodamine-conjugated antirabbit antibodies (green and red, respectively). To detect cellular and viral DNA, cells were stained with DAPI (blue).
    Secondary Antimouse Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher goat antimouse
    Phospho-JNK localization during VACV infection. BSC-40 cells were transfected with pcDNA3-cMyc-JNK2-MKK7 for 48 h, followed by infection with VACV for 7 h. At this time, cells were processed for fluorescence analyses and simultaneously stained with anti-c-Myc and antiviral protein I3 antibodies, followed by FITC-conjugated <t>antimouse</t> and rhodamine-conjugated antirabbit antibodies (green and red, respectively). To detect cellular and viral DNA, cells were stained with DAPI (blue).
    Goat Antimouse, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 363 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Thermo Fisher goat antimouse igg secondary antibodies
    Phospho-JNK localization during VACV infection. BSC-40 cells were transfected with pcDNA3-cMyc-JNK2-MKK7 for 48 h, followed by infection with VACV for 7 h. At this time, cells were processed for fluorescence analyses and simultaneously stained with anti-c-Myc and antiviral protein I3 antibodies, followed by FITC-conjugated <t>antimouse</t> and rhodamine-conjugated antirabbit antibodies (green and red, respectively). To detect cellular and viral DNA, cells were stained with DAPI (blue).
    Goat Antimouse Igg Secondary Antibodies, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher goat antimouse igg h l secondary antibody
    Phospho-JNK localization during VACV infection. BSC-40 cells were transfected with pcDNA3-cMyc-JNK2-MKK7 for 48 h, followed by infection with VACV for 7 h. At this time, cells were processed for fluorescence analyses and simultaneously stained with anti-c-Myc and antiviral protein I3 antibodies, followed by FITC-conjugated <t>antimouse</t> and rhodamine-conjugated antirabbit antibodies (green and red, respectively). To detect cellular and viral DNA, cells were stained with DAPI (blue).
    Goat Antimouse Igg H L Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher anti mouse igm secondary antibody
    Phospho-JNK localization during VACV infection. BSC-40 cells were transfected with pcDNA3-cMyc-JNK2-MKK7 for 48 h, followed by infection with VACV for 7 h. At this time, cells were processed for fluorescence analyses and simultaneously stained with anti-c-Myc and antiviral protein I3 antibodies, followed by FITC-conjugated <t>antimouse</t> and rhodamine-conjugated antirabbit antibodies (green and red, respectively). To detect cellular and viral DNA, cells were stained with DAPI (blue).
    Anti Mouse Igm Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher immunoglobulin g polyclonal rabbit antimouse antibody
    Phospho-JNK localization during VACV infection. BSC-40 cells were transfected with pcDNA3-cMyc-JNK2-MKK7 for 48 h, followed by infection with VACV for 7 h. At this time, cells were processed for fluorescence analyses and simultaneously stained with anti-c-Myc and antiviral protein I3 antibodies, followed by FITC-conjugated <t>antimouse</t> and rhodamine-conjugated antirabbit antibodies (green and red, respectively). To detect cellular and viral DNA, cells were stained with DAPI (blue).
    Immunoglobulin G Polyclonal Rabbit Antimouse Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Thermo Fisher secondary rabbit antimouse igg antibody
    Phospho-JNK localization during VACV infection. BSC-40 cells were transfected with pcDNA3-cMyc-JNK2-MKK7 for 48 h, followed by infection with VACV for 7 h. At this time, cells were processed for fluorescence analyses and simultaneously stained with anti-c-Myc and antiviral protein I3 antibodies, followed by FITC-conjugated <t>antimouse</t> and rhodamine-conjugated antirabbit antibodies (green and red, respectively). To detect cellular and viral DNA, cells were stained with DAPI (blue).
    Secondary Rabbit Antimouse Igg Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Bioworld Antibodies anti mouse secondary antibodies
    Phospho-JNK localization during VACV infection. BSC-40 cells were transfected with pcDNA3-cMyc-JNK2-MKK7 for 48 h, followed by infection with VACV for 7 h. At this time, cells were processed for fluorescence analyses and simultaneously stained with anti-c-Myc and antiviral protein I3 antibodies, followed by FITC-conjugated <t>antimouse</t> and rhodamine-conjugated antirabbit antibodies (green and red, respectively). To detect cellular and viral DNA, cells were stained with DAPI (blue).
    Anti Mouse Secondary Antibodies, supplied by Bioworld Antibodies, used in various techniques. Bioz Stars score: 90/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    GE Healthcare anti mouse secondary antibodies
    Colocalization of Nramp2 and transferrin in early endosomes was determined by double immunofluorescence and confocal microscopy in RAW macrophages transfected with a c-myc –tagged Nramp2 (A–C) and in untransfected control cells (D–F). Cells were cultured in the presence of FITC–conjugated transferrin before fixation and immunostaining with the primary anti– c-myc tag antibody (9E10) and a secondary rhodamine-conjugated, <t>anti–mouse</t> antibody. The slides were then examined by confocal microscopy, and the FITC (green; B and E) and rhodamine (red; A and D) images were overlaid to identify colocalization (C and F). The image in C shows colocalization of Nramp2 and FITC–transferrin in several of the cells in the field.
    Anti Mouse Secondary Antibodies, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 837 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Jackson Immuno anti mouse secondary antibodies
    Effect of DR6 deficiency on B cell proliferation, cell division, and survival. B cells isolated from spleens of 8–10-wk-old DR6-deficient (DR6 −/− ) and WT littermate control mice were stimulated with 20 μg/ml anti-IgM (A), 10 μg/ml anti-CD40 (B), or 5 μg/ml LPS (C) as described in Materials and Methods. Cells were cultured in triplicates in 96-well plates for 72 h and B cell proliferation was measured by [ 3 H]thymidine incorporation during the final 12 h of culture. Values shown represent the mean and error bars represent the SD. *, P
    Anti Mouse Secondary Antibodies, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 92/100, based on 901 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Promega anti mouse secondary antibody
    Effect of DR6 deficiency on B cell proliferation, cell division, and survival. B cells isolated from spleens of 8–10-wk-old DR6-deficient (DR6 −/− ) and WT littermate control mice were stimulated with 20 μg/ml anti-IgM (A), 10 μg/ml anti-CD40 (B), or 5 μg/ml LPS (C) as described in Materials and Methods. Cells were cultured in triplicates in 96-well plates for 72 h and B cell proliferation was measured by [ 3 H]thymidine incorporation during the final 12 h of culture. Values shown represent the mean and error bars represent the SD. *, P
    Anti Mouse Secondary Antibody, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Sangon Biotech anti mouse secondary antibody
    Effect of DR6 deficiency on B cell proliferation, cell division, and survival. B cells isolated from spleens of 8–10-wk-old DR6-deficient (DR6 −/− ) and WT littermate control mice were stimulated with 20 μg/ml anti-IgM (A), 10 μg/ml anti-CD40 (B), or 5 μg/ml LPS (C) as described in Materials and Methods. Cells were cultured in triplicates in 96-well plates for 72 h and B cell proliferation was measured by [ 3 H]thymidine incorporation during the final 12 h of culture. Values shown represent the mean and error bars represent the SD. *, P
    Anti Mouse Secondary Antibody, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Agilent technologies anti mouse secondary antibodies
    Effect of DR6 deficiency on B cell proliferation, cell division, and survival. B cells isolated from spleens of 8–10-wk-old DR6-deficient (DR6 −/− ) and WT littermate control mice were stimulated with 20 μg/ml anti-IgM (A), 10 μg/ml anti-CD40 (B), or 5 μg/ml LPS (C) as described in Materials and Methods. Cells were cultured in triplicates in 96-well plates for 72 h and B cell proliferation was measured by [ 3 H]thymidine incorporation during the final 12 h of culture. Values shown represent the mean and error bars represent the SD. *, P
    Anti Mouse Secondary Antibodies, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 420 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Becton Dickinson anti mouse secondary antibodies
    Effect of DR6 deficiency on B cell proliferation, cell division, and survival. B cells isolated from spleens of 8–10-wk-old DR6-deficient (DR6 −/− ) and WT littermate control mice were stimulated with 20 μg/ml anti-IgM (A), 10 μg/ml anti-CD40 (B), or 5 μg/ml LPS (C) as described in Materials and Methods. Cells were cultured in triplicates in 96-well plates for 72 h and B cell proliferation was measured by [ 3 H]thymidine incorporation during the final 12 h of culture. Values shown represent the mean and error bars represent the SD. *, P
    Anti Mouse Secondary Antibodies, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Phospho-JNK localization during VACV infection. BSC-40 cells were transfected with pcDNA3-cMyc-JNK2-MKK7 for 48 h, followed by infection with VACV for 7 h. At this time, cells were processed for fluorescence analyses and simultaneously stained with anti-c-Myc and antiviral protein I3 antibodies, followed by FITC-conjugated antimouse and rhodamine-conjugated antirabbit antibodies (green and red, respectively). To detect cellular and viral DNA, cells were stained with DAPI (blue).

    Journal: Journal of Virology

    Article Title: A Vaccinia Virus-Driven Interplay between the MKK4/7-JNK1/2 Pathway and Cytoskeleton Reorganization

    doi: 10.1128/JVI.05638-11

    Figure Lengend Snippet: Phospho-JNK localization during VACV infection. BSC-40 cells were transfected with pcDNA3-cMyc-JNK2-MKK7 for 48 h, followed by infection with VACV for 7 h. At this time, cells were processed for fluorescence analyses and simultaneously stained with anti-c-Myc and antiviral protein I3 antibodies, followed by FITC-conjugated antimouse and rhodamine-conjugated antirabbit antibodies (green and red, respectively). To detect cellular and viral DNA, cells were stained with DAPI (blue).

    Article Snippet: Rhodamine-conjugated phalloidin, rhodamine-conjugated antirabbit and antimouse secondary antibodies, and fluorescein isothiocyanate (FITC)-conjugated antimouse secondary antibody were purchased from Invitrogen-Molecular Probes (Carlsbad, CA).

    Techniques: Infection, Transfection, Fluorescence, Staining

    VACV-infected JNK1/2 −/− MEFs exhibit reduced cell motility. (A) WT and JNK1/2-KO MEFs were infected with VACV at an MOI of 5 for 3, 6, or 12 h. Cells were then fixed, permeabilized, blocked, and stained with mouse anti-β-tubulin, followed by FITC-conjugated antimouse secondary antibody and rhodamine-conjugated phalloidin. (Panels a to c) Representative image of the migratory morphotype 7; (panel b) arrow, the trailing edge; bracket, the lamellipodia. The graphic representation of the relative abundance of this phenotype in WT or JNK1/2 −/− VACV-infected cells is shown at the bottom of panel A ( n > 100). (B) Phase-contrast images of scratched confluent monolayers of WT or JNK1/2 −/− MEFs infected with VACV (MOI, 5). Cells were fixed at 0, 6, 12, and 24 hpi and processed for microscopic analysis. (C) Graphic representations of the number of migrating cells per 5 mm in the wounded area of confluent monolayers of WT, JNK1/2 −/− , or MKK4/7 −/− MEFs as well as WT cells treated with JNKi (4 μM) and then infected with VACV at an MOI of 5. *, P

    Journal: Journal of Virology

    Article Title: A Vaccinia Virus-Driven Interplay between the MKK4/7-JNK1/2 Pathway and Cytoskeleton Reorganization

    doi: 10.1128/JVI.05638-11

    Figure Lengend Snippet: VACV-infected JNK1/2 −/− MEFs exhibit reduced cell motility. (A) WT and JNK1/2-KO MEFs were infected with VACV at an MOI of 5 for 3, 6, or 12 h. Cells were then fixed, permeabilized, blocked, and stained with mouse anti-β-tubulin, followed by FITC-conjugated antimouse secondary antibody and rhodamine-conjugated phalloidin. (Panels a to c) Representative image of the migratory morphotype 7; (panel b) arrow, the trailing edge; bracket, the lamellipodia. The graphic representation of the relative abundance of this phenotype in WT or JNK1/2 −/− VACV-infected cells is shown at the bottom of panel A ( n > 100). (B) Phase-contrast images of scratched confluent monolayers of WT or JNK1/2 −/− MEFs infected with VACV (MOI, 5). Cells were fixed at 0, 6, 12, and 24 hpi and processed for microscopic analysis. (C) Graphic representations of the number of migrating cells per 5 mm in the wounded area of confluent monolayers of WT, JNK1/2 −/− , or MKK4/7 −/− MEFs as well as WT cells treated with JNKi (4 μM) and then infected with VACV at an MOI of 5. *, P

    Article Snippet: Rhodamine-conjugated phalloidin, rhodamine-conjugated antirabbit and antimouse secondary antibodies, and fluorescein isothiocyanate (FITC)-conjugated antimouse secondary antibody were purchased from Invitrogen-Molecular Probes (Carlsbad, CA).

    Techniques: Infection, Staining

    Viral trafficking to the cell periphery is deregulated in JNK1/2-KO cells. (A) Detection of CEV in the cell surface by confocal microscopy. WT and JNK1/2-KO MEFs were infected with VACV vF13L-GFP at an MOI of 5 for 9 h. Cells were fixed and blocked without permeabilization and then incubated with mouse anti-B5 antibody, followed by staining with rhodamine-conjugated antimouse secondary antibody. White arrows indicate CEVs. Micrographs are shown with their scales indicated by the bars. (B) Absence of JNK1/2 affects the release of EEV from the infected cells. Culture supernatants of VACV-infected WT and JNK1/2/KO cells at an MOI of 10 for 48 h were incubated with the anti-IMV-specific L1R antibody (1:1,000) at 37°C for 1 h and then titrated in BSC-40 cells. Data are representative of at least three independent experiments with similar results (**, P

    Journal: Journal of Virology

    Article Title: A Vaccinia Virus-Driven Interplay between the MKK4/7-JNK1/2 Pathway and Cytoskeleton Reorganization

    doi: 10.1128/JVI.05638-11

    Figure Lengend Snippet: Viral trafficking to the cell periphery is deregulated in JNK1/2-KO cells. (A) Detection of CEV in the cell surface by confocal microscopy. WT and JNK1/2-KO MEFs were infected with VACV vF13L-GFP at an MOI of 5 for 9 h. Cells were fixed and blocked without permeabilization and then incubated with mouse anti-B5 antibody, followed by staining with rhodamine-conjugated antimouse secondary antibody. White arrows indicate CEVs. Micrographs are shown with their scales indicated by the bars. (B) Absence of JNK1/2 affects the release of EEV from the infected cells. Culture supernatants of VACV-infected WT and JNK1/2/KO cells at an MOI of 10 for 48 h were incubated with the anti-IMV-specific L1R antibody (1:1,000) at 37°C for 1 h and then titrated in BSC-40 cells. Data are representative of at least three independent experiments with similar results (**, P

    Article Snippet: Rhodamine-conjugated phalloidin, rhodamine-conjugated antirabbit and antimouse secondary antibodies, and fluorescein isothiocyanate (FITC)-conjugated antimouse secondary antibody were purchased from Invitrogen-Molecular Probes (Carlsbad, CA).

    Techniques: Confocal Microscopy, Infection, Incubation, Staining

    VACV-infected JNK1/2 −/− MEFS exhibit reduced early cell contractility. WT (a to l) and JNK1/2-KO (m to x) MEFs were infected with VACV WR (MOI, 5). At 3, 6, or 12 hpi, cells were fixed and stained for the microtubule and actin networks. β-Tubulin, F-actin, and the nucleus are stained with FITC-conjugated antimouse secondary antibody (green), rhodamine conjugated-phalloidin (red), and DAPI (blue), respectively. Fluorescently labeled cells were visualized using a Zeiss (LSM 510 META) confocal microscope. The graphic representations of the relative abundance of each phenotype found in WT-infected cells (graphs 1 to 3, f, i, and l) or JNK1/2 −/− -infected cells (graphs 4 to 6, r, u, and x) are shown on the right ( n > 100). (Left column) Thin arrows, microtubule projections (j); large arrows, microtubule long protrusions (v); (middle column) insets, cortical F-actin in detail, highlighting the presence (b, h, n, q, and t) or absence (e, k, and w) of stress fibers; dashed arrows, actin tails (h and t); (right column) insets, the edge of cell protrusions in detail, highlighting the protraction (c, i, l, o, r, u, and x) or retraction (f) of microtubule. Insets represent enlargement of the area indicated by the white box in the panel.

    Journal: Journal of Virology

    Article Title: A Vaccinia Virus-Driven Interplay between the MKK4/7-JNK1/2 Pathway and Cytoskeleton Reorganization

    doi: 10.1128/JVI.05638-11

    Figure Lengend Snippet: VACV-infected JNK1/2 −/− MEFS exhibit reduced early cell contractility. WT (a to l) and JNK1/2-KO (m to x) MEFs were infected with VACV WR (MOI, 5). At 3, 6, or 12 hpi, cells were fixed and stained for the microtubule and actin networks. β-Tubulin, F-actin, and the nucleus are stained with FITC-conjugated antimouse secondary antibody (green), rhodamine conjugated-phalloidin (red), and DAPI (blue), respectively. Fluorescently labeled cells were visualized using a Zeiss (LSM 510 META) confocal microscope. The graphic representations of the relative abundance of each phenotype found in WT-infected cells (graphs 1 to 3, f, i, and l) or JNK1/2 −/− -infected cells (graphs 4 to 6, r, u, and x) are shown on the right ( n > 100). (Left column) Thin arrows, microtubule projections (j); large arrows, microtubule long protrusions (v); (middle column) insets, cortical F-actin in detail, highlighting the presence (b, h, n, q, and t) or absence (e, k, and w) of stress fibers; dashed arrows, actin tails (h and t); (right column) insets, the edge of cell protrusions in detail, highlighting the protraction (c, i, l, o, r, u, and x) or retraction (f) of microtubule. Insets represent enlargement of the area indicated by the white box in the panel.

    Article Snippet: Rhodamine-conjugated phalloidin, rhodamine-conjugated antirabbit and antimouse secondary antibodies, and fluorescein isothiocyanate (FITC)-conjugated antimouse secondary antibody were purchased from Invitrogen-Molecular Probes (Carlsbad, CA).

    Techniques: Infection, Staining, Labeling, Microscopy

    Colocalization of Nramp2 and transferrin in early endosomes was determined by double immunofluorescence and confocal microscopy in RAW macrophages transfected with a c-myc –tagged Nramp2 (A–C) and in untransfected control cells (D–F). Cells were cultured in the presence of FITC–conjugated transferrin before fixation and immunostaining with the primary anti– c-myc tag antibody (9E10) and a secondary rhodamine-conjugated, anti–mouse antibody. The slides were then examined by confocal microscopy, and the FITC (green; B and E) and rhodamine (red; A and D) images were overlaid to identify colocalization (C and F). The image in C shows colocalization of Nramp2 and FITC–transferrin in several of the cells in the field.

    Journal: The Journal of Experimental Medicine

    Article Title: The Iron Transport Protein NRAMP2 Is an Integral Membrane Glycoprotein That Colocalizes with Transferrin in Recycling Endosomes

    doi:

    Figure Lengend Snippet: Colocalization of Nramp2 and transferrin in early endosomes was determined by double immunofluorescence and confocal microscopy in RAW macrophages transfected with a c-myc –tagged Nramp2 (A–C) and in untransfected control cells (D–F). Cells were cultured in the presence of FITC–conjugated transferrin before fixation and immunostaining with the primary anti– c-myc tag antibody (9E10) and a secondary rhodamine-conjugated, anti–mouse antibody. The slides were then examined by confocal microscopy, and the FITC (green; B and E) and rhodamine (red; A and D) images were overlaid to identify colocalization (C and F). The image in C shows colocalization of Nramp2 and FITC–transferrin in several of the cells in the field.

    Article Snippet: Anti–rabbit, anti–rat, and anti–mouse secondary antibodies conjugated to horseradish peroxidase were used at 1:10,000 ( Amersham ).

    Techniques: Immunofluorescence, Confocal Microscopy, Transfection, Cell Culture, Immunostaining

    Effect of DR6 deficiency on B cell proliferation, cell division, and survival. B cells isolated from spleens of 8–10-wk-old DR6-deficient (DR6 −/− ) and WT littermate control mice were stimulated with 20 μg/ml anti-IgM (A), 10 μg/ml anti-CD40 (B), or 5 μg/ml LPS (C) as described in Materials and Methods. Cells were cultured in triplicates in 96-well plates for 72 h and B cell proliferation was measured by [ 3 H]thymidine incorporation during the final 12 h of culture. Values shown represent the mean and error bars represent the SD. *, P

    Journal: The Journal of Experimental Medicine

    Article Title: Enhanced B Cell Expansion, Survival, and Humoral Responses by Targeting Death Receptor 6

    doi: 10.1084/jem.20020617

    Figure Lengend Snippet: Effect of DR6 deficiency on B cell proliferation, cell division, and survival. B cells isolated from spleens of 8–10-wk-old DR6-deficient (DR6 −/− ) and WT littermate control mice were stimulated with 20 μg/ml anti-IgM (A), 10 μg/ml anti-CD40 (B), or 5 μg/ml LPS (C) as described in Materials and Methods. Cells were cultured in triplicates in 96-well plates for 72 h and B cell proliferation was measured by [ 3 H]thymidine incorporation during the final 12 h of culture. Values shown represent the mean and error bars represent the SD. *, P

    Article Snippet: C-Rel, Bcl-xL , and β actin proteins were detected with horseradish peroxidase–conjugated anti–rabbit or anti–mouse secondary antibodies (Jackson ImmunoResearch Laboratories) and developed by SuperSignal® West Pico Chemiluminescent Substrate kit (Pierce Chemical Co.).

    Techniques: Isolation, Mouse Assay, Cell Culture

    Increased nuclear levels and activity of c-Rel and elevated Bcl-x L expression in activated DR6 −/− B cells. (A) Western blot analysis of c-Rel in cytoplasmic and nuclear extracts from DR6-deficient (−/−) and WT (wt) B cell cultures. Purified splenic B cells were stimulated with or without 10 μg/ml anti-IgM or 1 μg/ml anti-CD40 for 30 min or 4 h as indicated. Cytoplasmic (C) and nuclear (N) extracts were prepared as described in Materials and Methods. C-Rel was detected with specific anti–c-Rel Ab after SDS-PAGE. Equal amounts of either cytoplasmic or nuclear protein extracts were loaded into wells and data shown are representative of two independent experiments. (B) GMSA with nuclear fractions of DR6 −/− and WT B cells activated with anti-IgM or anti-CD40 for 0.5 or 4 h as described in Materials and Methods. Nuclear c-Rel from activated DR6 −/− B cells bound to its specific DNA probe (top) and supershifted with the addition of anti–c-Rel antibody (bottom). Middle bands were nonspecific binding, as they were not competed by cold homologous oligonucleotide. (C) B cells from DR6-deficient (−/−) or WT (wt) were either untreated or treated with anti-IgM or anti-CD40 for 4 h and total cell lysates were prepared in 1× RIPA buffer as described in Materials and Methods. After SDS-PAGE and Western transfer, Bcl-x L in anti-CD40–stimulated samples was detected with rabbit polyclonal anti–Bcl-x L , whereas mouse monoclonal anti–Bcl-x L was used to detect the protein in anti-IgM–treated samples as the stimulus was a rabbit anti–mouse IgM antibody that was detected by the original secondary, anti–rabbit antibody. All blots were probed with mouse anti–β actin as a loading control. Data shown are representative of two independent experiments.

    Journal: The Journal of Experimental Medicine

    Article Title: Enhanced B Cell Expansion, Survival, and Humoral Responses by Targeting Death Receptor 6

    doi: 10.1084/jem.20020617

    Figure Lengend Snippet: Increased nuclear levels and activity of c-Rel and elevated Bcl-x L expression in activated DR6 −/− B cells. (A) Western blot analysis of c-Rel in cytoplasmic and nuclear extracts from DR6-deficient (−/−) and WT (wt) B cell cultures. Purified splenic B cells were stimulated with or without 10 μg/ml anti-IgM or 1 μg/ml anti-CD40 for 30 min or 4 h as indicated. Cytoplasmic (C) and nuclear (N) extracts were prepared as described in Materials and Methods. C-Rel was detected with specific anti–c-Rel Ab after SDS-PAGE. Equal amounts of either cytoplasmic or nuclear protein extracts were loaded into wells and data shown are representative of two independent experiments. (B) GMSA with nuclear fractions of DR6 −/− and WT B cells activated with anti-IgM or anti-CD40 for 0.5 or 4 h as described in Materials and Methods. Nuclear c-Rel from activated DR6 −/− B cells bound to its specific DNA probe (top) and supershifted with the addition of anti–c-Rel antibody (bottom). Middle bands were nonspecific binding, as they were not competed by cold homologous oligonucleotide. (C) B cells from DR6-deficient (−/−) or WT (wt) were either untreated or treated with anti-IgM or anti-CD40 for 4 h and total cell lysates were prepared in 1× RIPA buffer as described in Materials and Methods. After SDS-PAGE and Western transfer, Bcl-x L in anti-CD40–stimulated samples was detected with rabbit polyclonal anti–Bcl-x L , whereas mouse monoclonal anti–Bcl-x L was used to detect the protein in anti-IgM–treated samples as the stimulus was a rabbit anti–mouse IgM antibody that was detected by the original secondary, anti–rabbit antibody. All blots were probed with mouse anti–β actin as a loading control. Data shown are representative of two independent experiments.

    Article Snippet: C-Rel, Bcl-xL , and β actin proteins were detected with horseradish peroxidase–conjugated anti–rabbit or anti–mouse secondary antibodies (Jackson ImmunoResearch Laboratories) and developed by SuperSignal® West Pico Chemiluminescent Substrate kit (Pierce Chemical Co.).

    Techniques: Activity Assay, Expressing, Western Blot, Purification, SDS Page, Binding Assay