Journal: Nature Communications

Article Title: Pericyte loss influences Alzheimer-like neurodegeneration in mice

doi: 10.1038/ncomms3932

Figure Lengend Snippet: Accelerated blood–brain barrier breakdown and microvascular reductions in pericyte-deficient APP sw/0 Pdgfrβ +/− mice. ( a , b ) Western blot analysis of serum IgG in capillary-depleted cortical homogenates relative to β-actin ( a ) and quantification of IgG extravascular levels ( b ) in 1-month-old Pdgfrβ +/+ , Pdgfrβ +/− , APP sw/0 ; Pdgfrβ +/+ and APP sw/0 ; Pdgfrβ +/− mice. Full-size blots can be found in Supplementary Fig. S5 . ( c ) Total length of lectin-positive capillary profiles in 1-month-old Pdgfrβ +/+ , Pdgfrβ +/− , APP sw/0 ; Pdgfrβ +/+ and APP sw/0 ; Pdgfrβ +/− mice. In b , values are means±s.e.m., n =4 mice per group. In c , values are means±s.e.m., n =6 mice per group. P

Article Snippet: The following antibodies were used: anti-tau phospho Threonine 231, Millipore), anti-LRP-85 (Abcam), anti-RAGE (Santa Cruz Biotechnology Inc.), anti-neprilysin (R & D Systems), anti-IDE (Millipore, AB9210), anti-APP (Millipore) and horse radish peroxidase-conjugated goat anti-mouse IgG (Bio-Rad).

Techniques: Mouse Assay, Western Blot

Journal: Avicenna Journal of Medical Biotechnology

Article Title: Evaluation of Immune Responses Induced by GRA7 and ROP2 Genes by DNA Vaccine Cocktails Against Acute Toxoplasmosis in BALB/c Mice

doi:

Figure Lengend Snippet: Results for level of immunoglubin G subtypes evaluation, IgG1 and IgG2a subtypes in mice serum samples of different experimental groups assayed by ELISA.

Article Snippet: Briefly, microtiter plates coated with 10 μg/ml solution of total T. gondii antigens were used to capture antibodies in the mouse sera (sera were diluted 1/100 in 5% dried skimmed milk) and incubated for 2 hr at 37°C ., which were then detected with horse radish peroxidase-conjugated goat anti-mouse IgG, IgG1, or IgG2a (DAKO, Denmark) as secondary antibodies for isotype analysis.

Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay