anti-mouse igg Search Results


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  • 94
    Thermo Fisher antimouse igg antibodies
    Antimouse Igg Antibodies, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Millipore antimouse igg conjugate
    Antimouse Igg Conjugate, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Bio-Rad antimouse igg
    Antimouse Igg, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore antimouse igg
    Inhibition of angiogenesis by APN inhibitors. A , mice developing hypoxia-induced retinal neovasculature were treated i.v. with PBS ( Vehicle ), <t>antimouse</t> APN antibodies or normal rat <t>IgG</t> (250 μ g/mouse), or bestatin (200 μ g/mouse). The number of retinal neovessels in mice treated with PBS was set at 100%. Data represent means; n = 3; bars, SE. The reduction in blood vessel number was statistically significant for the anti-APN antibodies and bestatin ( P
    Antimouse Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore goat antimouse igg
    Expression of human leucocyte antigen (HLA)-B27 (a) and monomorphic major histocompatibility complex (MHC) class I (b) molecules on monocytes from seven patients with Salmonella -triggered reactive arthritis (ReA) who were positive for the HLA-B27 gene during the acute phase of infection (1–4 weeks, patient 3 after 8 weeks) and during the follow-up. *Patients ◊ and ▪ did not recover during the follow-up. The cells were incubated with monoclonal antibodies (mAbs) specific for HLA-B27 (HLA-ABC-m3) and monomorphic MHC class I (w6/32) and then with fluorescein isothiocyanate (FITC)-labelled F(ab′) 2 fragments of goat <t>antimouse</t> <t>IgG.</t> The y -axis is the mean fluorescence intensity (MFI) of the specific mAb. The MFI of the negative control mAb was subtracted from each value before plotting.
    Goat Antimouse Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 106 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad antimouse igg antibodies
    Expression of human leucocyte antigen (HLA)-B27 (a) and monomorphic major histocompatibility complex (MHC) class I (b) molecules on monocytes from seven patients with Salmonella -triggered reactive arthritis (ReA) who were positive for the HLA-B27 gene during the acute phase of infection (1–4 weeks, patient 3 after 8 weeks) and during the follow-up. *Patients ◊ and ▪ did not recover during the follow-up. The cells were incubated with monoclonal antibodies (mAbs) specific for HLA-B27 (HLA-ABC-m3) and monomorphic MHC class I (w6/32) and then with fluorescein isothiocyanate (FITC)-labelled F(ab′) 2 fragments of goat <t>antimouse</t> <t>IgG.</t> The y -axis is the mean fluorescence intensity (MFI) of the specific mAb. The MFI of the negative control mAb was subtracted from each value before plotting.
    Antimouse Igg Antibodies, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 91 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    GE Healthcare anti mouse igg2a antibodies
    Analysis of IgG1 (A) and <t>IgG2a</t> (B) antibody responses of BALB/c mice to the recombinant BFR and P39 antigens and to B. abortus 544 extract. Mice were immunized three times with BFR or P39 in different ODN combinations. Sera from four mice per group collected 3 weeks after the last immunization were assayed individually by ELISA. Antibody levels are expressed as mean titer.
    Anti Mouse Igg2a Antibodies, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad anti mouse igg isotype ab
    Analysis of IgG1 (A) and <t>IgG2a</t> (B) antibody responses of BALB/c mice to the recombinant BFR and P39 antigens and to B. abortus 544 extract. Mice were immunized three times with BFR or P39 in different ODN combinations. Sera from four mice per group collected 3 weeks after the last immunization were assayed individually by ELISA. Antibody levels are expressed as mean titer.
    Anti Mouse Igg Isotype Ab, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioLegend rat antimouse igg
    Analysis of IgG1 (A) and <t>IgG2a</t> (B) antibody responses of BALB/c mice to the recombinant BFR and P39 antigens and to B. abortus 544 extract. Mice were immunized three times with BFR or P39 in different ODN combinations. Sera from four mice per group collected 3 weeks after the last immunization were assayed individually by ELISA. Antibody levels are expressed as mean titer.
    Rat Antimouse Igg, supplied by BioLegend, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Abcam goat antimouse igg
    Dye-labeled <t>IgG</t> can be used as a probe to detect dilute protein in solution. Strips of PVDF were submerged in solutions of varying concentrations of human serum albumin in PBS buffer. Albumin was then probed for using mouse antihuman albumin, followed by D0 dimer-labeled goat <t>antimouse</t> IgG. The PVDF strips were exposed to PROTOGOLD and LI silver stain solutions, and analyzed by Raman spectroscopy at 532 nm. The resulting spectra were baseline corrected and vector normalized to equivalent scales using OPUS 7.5 software.
    Goat Antimouse Igg, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cell Signaling Technology Inc antimouse igg
    Dye-labeled <t>IgG</t> can be used as a probe to detect dilute protein in solution. Strips of PVDF were submerged in solutions of varying concentrations of human serum albumin in PBS buffer. Albumin was then probed for using mouse antihuman albumin, followed by D0 dimer-labeled goat <t>antimouse</t> IgG. The PVDF strips were exposed to PROTOGOLD and LI silver stain solutions, and analyzed by Raman spectroscopy at 532 nm. The resulting spectra were baseline corrected and vector normalized to equivalent scales using OPUS 7.5 software.
    Antimouse Igg, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher antimouse igg dynabeads
    Dye-labeled <t>IgG</t> can be used as a probe to detect dilute protein in solution. Strips of PVDF were submerged in solutions of varying concentrations of human serum albumin in PBS buffer. Albumin was then probed for using mouse antihuman albumin, followed by D0 dimer-labeled goat <t>antimouse</t> IgG. The PVDF strips were exposed to PROTOGOLD and LI silver stain solutions, and analyzed by Raman spectroscopy at 532 nm. The resulting spectra were baseline corrected and vector normalized to equivalent scales using OPUS 7.5 software.
    Antimouse Igg Dynabeads, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Abcam antimouse igg
    (a) Schematic diagram of the enzyme-linked immunosorbent assay (ELISA) performed on the PONB nanofiber meshes using streptavidin bridges to connect the biotinylated surface to a biotinylated <t>antimouse</t> <t>IgG.</t> A generic mouse IgG was used as the target molecule,
    Antimouse Igg, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    R&D Systems goat anti mouse igg ab
    (a) Schematic diagram of the enzyme-linked immunosorbent assay (ELISA) performed on the PONB nanofiber meshes using streptavidin bridges to connect the biotinylated surface to a biotinylated <t>antimouse</t> <t>IgG.</t> A generic mouse IgG was used as the target molecule,
    Goat Anti Mouse Igg Ab, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Jackson Immuno anti mouse igg antibodies
    Binding and blocking of BNeV VLPs to synthetic HBGAs. (A) Ninety-six-well plates were coated with 50 μg/ml BNeV VLPs, GST-tagged-VP8* protein and GST-tagged P particles (10 μg/ml), supernatant of wild-type baculovirus-infected Sf9 cells lysate, and GST and then incubated with each of the synthetic HBGAs (10 μg/ml). The binding of each HBGA to target viral proteins and the control was determined by addition of horseradish peroxidase-conjugated <t>streptavidin</t> as described in Materials and Methods. (B) α1,2-Linked fucose, α1,3/4-linked fucose, αGal, and GalNAc epitopes were removed from each synthetic HBGA coated in each well using the corresponding enzyme. After incubation of BNeV VLPs at 50 μg/ml, the binding of BNeV VLPs was determined using hyperimmune serum against BNeV capsid protein, followed by addition of horseradish peroxidase-conjugated goat anti-rabbit <t>IgG</t> antibody. The signal intensities for graphs in panels A and B were visualized using TMB at 450 nm in three independent experiments. Error bars indicate SD from triplicate samples.
    Anti Mouse Igg Antibodies, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 99/100, based on 305 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore anti mouse igg antibodies
    Expression of TCI gene in 293T cells. Transfected cells were incubated with Mabs against the HIV‐1 p24 protein and HRP‐conjugated rabbit <t>anti‐mouse</t> <t>IgG;</t> complex was visualized by staining with a substrate solution containing 3.3′‐diaminobenzidine tetrahydrochloride: (A) 293T cells transfected with pcDNA‐TCI and (B) 293T cells transfected with pcDNA3.1. Cells were visualized by microscopy with an Axioskop 2 (Carl Zeiss).
    Anti Mouse Igg Antibodies, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 324 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Inhibition of angiogenesis by APN inhibitors. A , mice developing hypoxia-induced retinal neovasculature were treated i.v. with PBS ( Vehicle ), antimouse APN antibodies or normal rat IgG (250 μ g/mouse), or bestatin (200 μ g/mouse). The number of retinal neovessels in mice treated with PBS was set at 100%. Data represent means; n = 3; bars, SE. The reduction in blood vessel number was statistically significant for the anti-APN antibodies and bestatin ( P

    Journal: Cancer research

    Article Title: Aminopeptidase N Is a Receptor for Tumor-homing Peptides and a Target for Inhibiting Angiogenesis

    doi:

    Figure Lengend Snippet: Inhibition of angiogenesis by APN inhibitors. A , mice developing hypoxia-induced retinal neovasculature were treated i.v. with PBS ( Vehicle ), antimouse APN antibodies or normal rat IgG (250 μ g/mouse), or bestatin (200 μ g/mouse). The number of retinal neovessels in mice treated with PBS was set at 100%. Data represent means; n = 3; bars, SE. The reduction in blood vessel number was statistically significant for the anti-APN antibodies and bestatin ( P

    Article Snippet: Alternatively, fluorescently labeled antimouse IgG (Sigma) was used for detecting the primary antibody.

    Techniques: Inhibition, Mouse Assay

    Expression of human leucocyte antigen (HLA)-B27 (a) and monomorphic major histocompatibility complex (MHC) class I (b) molecules on monocytes from seven patients with Salmonella -triggered reactive arthritis (ReA) who were positive for the HLA-B27 gene during the acute phase of infection (1–4 weeks, patient 3 after 8 weeks) and during the follow-up. *Patients ◊ and ▪ did not recover during the follow-up. The cells were incubated with monoclonal antibodies (mAbs) specific for HLA-B27 (HLA-ABC-m3) and monomorphic MHC class I (w6/32) and then with fluorescein isothiocyanate (FITC)-labelled F(ab′) 2 fragments of goat antimouse IgG. The y -axis is the mean fluorescence intensity (MFI) of the specific mAb. The MFI of the negative control mAb was subtracted from each value before plotting.

    Journal: Immunology

    Article Title: Enterobacterial infection modulates major histocompatibility complex class I expression on mononuclear cells

    doi: 10.1046/j.1365-2567.1999.00803.x

    Figure Lengend Snippet: Expression of human leucocyte antigen (HLA)-B27 (a) and monomorphic major histocompatibility complex (MHC) class I (b) molecules on monocytes from seven patients with Salmonella -triggered reactive arthritis (ReA) who were positive for the HLA-B27 gene during the acute phase of infection (1–4 weeks, patient 3 after 8 weeks) and during the follow-up. *Patients ◊ and ▪ did not recover during the follow-up. The cells were incubated with monoclonal antibodies (mAbs) specific for HLA-B27 (HLA-ABC-m3) and monomorphic MHC class I (w6/32) and then with fluorescein isothiocyanate (FITC)-labelled F(ab′) 2 fragments of goat antimouse IgG. The y -axis is the mean fluorescence intensity (MFI) of the specific mAb. The MFI of the negative control mAb was subtracted from each value before plotting.

    Article Snippet: Cells were then washed twice and incubated with fluorescein isothiocyanate (FITC)-labelled F(ab′)2 fragments of goat antimouse IgG (1:200 v/v) at +4° for 15 min (Sigma Chemical Company, St Louis, MO).

    Techniques: Expressing, Infection, Incubation, Fluorescence, Negative Control

    (a) Expression of human leucocyte antigen (HLA)-B27 and major histocompatibility complex (MHC) class I molecules on monocytes from the patient with uncomplicated Salmonella infection. The cells were incubated with monoclonal antibodies (mAbs) specific for HLA-B27 or with negative control mAbs, and then with fluorescein isothiocyanate (FITC)-labelled F(ab′) 2 fragments of goat antimouse IgG. The expression of HLA-B27 detected with mAb HLA-ABC-m3 is shown as black histograms and corresponding negative controls as white histograms. The x -axis is the mean fluorescence intensity (MFI) on a log scale and the y -axis is the relative number of the cells. (b) Expression of monomorphic MHC class I on monocytes from patients with S. enteritidis infection without reactive arthritis (ReA). The cells were incubated with mAb recognizing monomorphic MHC class I and then with FITC-labelled F(ab′) 2 fragments of goat antimouse IgG. The y -axis is the MFI of the specific mAb. The MFI of the negative control mAb was subtracted from each value before plotting. (c) Expression of HLA-ABC-m3 and FD705 epitopes of HLA-B27 on monocytes from a healthy control subject (A, B and C). The second sample (B) was taken 5 weeks and the third sample (C) 1 year after the first sample (A).

    Journal: Immunology

    Article Title: Enterobacterial infection modulates major histocompatibility complex class I expression on mononuclear cells

    doi: 10.1046/j.1365-2567.1999.00803.x

    Figure Lengend Snippet: (a) Expression of human leucocyte antigen (HLA)-B27 and major histocompatibility complex (MHC) class I molecules on monocytes from the patient with uncomplicated Salmonella infection. The cells were incubated with monoclonal antibodies (mAbs) specific for HLA-B27 or with negative control mAbs, and then with fluorescein isothiocyanate (FITC)-labelled F(ab′) 2 fragments of goat antimouse IgG. The expression of HLA-B27 detected with mAb HLA-ABC-m3 is shown as black histograms and corresponding negative controls as white histograms. The x -axis is the mean fluorescence intensity (MFI) on a log scale and the y -axis is the relative number of the cells. (b) Expression of monomorphic MHC class I on monocytes from patients with S. enteritidis infection without reactive arthritis (ReA). The cells were incubated with mAb recognizing monomorphic MHC class I and then with FITC-labelled F(ab′) 2 fragments of goat antimouse IgG. The y -axis is the MFI of the specific mAb. The MFI of the negative control mAb was subtracted from each value before plotting. (c) Expression of HLA-ABC-m3 and FD705 epitopes of HLA-B27 on monocytes from a healthy control subject (A, B and C). The second sample (B) was taken 5 weeks and the third sample (C) 1 year after the first sample (A).

    Article Snippet: Cells were then washed twice and incubated with fluorescein isothiocyanate (FITC)-labelled F(ab′)2 fragments of goat antimouse IgG (1:200 v/v) at +4° for 15 min (Sigma Chemical Company, St Louis, MO).

    Techniques: Expressing, Infection, Incubation, Negative Control, Fluorescence

    (a) Expression of human leucocyte antigen (HLA)-B27, HLA-A2, HLA-B7 and major histocompatibility complex (MHC) class I molecules on monocytes from patient 5 (P5) with Salmonella -triggered reactive arthritis (ReA) at the start of the infection and during the follow-up. The cells were incubated with monoclonal antibodies (mAbs) specific for HLA-B27 (three mAbs against different epitopes of HLA-B27), HLA-A2, HLA-B7 and monomorphic MHC class I and then with fluorescein isothiocyanate (FITC)-labelled F(ab′) 2 fragments of goat antimouse IgG. The expression of MHC class I epitopes recognized with mAbs is shown as black histograms and corresponding negative controls as white histograms. The x -axis is the mean fluorescence intensity (MFI) on a log scale, and the y -axis is the relative number of the cells. (b) Expression of HLA-B27 and monomorphic MHC class I epitopes on monocytes from a patient with Yersinia -triggered ReA. The cells were incubated with mAbs specific for HLA-B27 (four mAbs against different epitopes of HLA-B27) and monomorphic MHC class I and then with FITC-labelled F(ab′) 2 fragments of goat antimouse IgG.

    Journal: Immunology

    Article Title: Enterobacterial infection modulates major histocompatibility complex class I expression on mononuclear cells

    doi: 10.1046/j.1365-2567.1999.00803.x

    Figure Lengend Snippet: (a) Expression of human leucocyte antigen (HLA)-B27, HLA-A2, HLA-B7 and major histocompatibility complex (MHC) class I molecules on monocytes from patient 5 (P5) with Salmonella -triggered reactive arthritis (ReA) at the start of the infection and during the follow-up. The cells were incubated with monoclonal antibodies (mAbs) specific for HLA-B27 (three mAbs against different epitopes of HLA-B27), HLA-A2, HLA-B7 and monomorphic MHC class I and then with fluorescein isothiocyanate (FITC)-labelled F(ab′) 2 fragments of goat antimouse IgG. The expression of MHC class I epitopes recognized with mAbs is shown as black histograms and corresponding negative controls as white histograms. The x -axis is the mean fluorescence intensity (MFI) on a log scale, and the y -axis is the relative number of the cells. (b) Expression of HLA-B27 and monomorphic MHC class I epitopes on monocytes from a patient with Yersinia -triggered ReA. The cells were incubated with mAbs specific for HLA-B27 (four mAbs against different epitopes of HLA-B27) and monomorphic MHC class I and then with FITC-labelled F(ab′) 2 fragments of goat antimouse IgG.

    Article Snippet: Cells were then washed twice and incubated with fluorescein isothiocyanate (FITC)-labelled F(ab′)2 fragments of goat antimouse IgG (1:200 v/v) at +4° for 15 min (Sigma Chemical Company, St Louis, MO).

    Techniques: Expressing, Infection, Incubation, Fluorescence

    Mitochondrial CA (mCA) in the primary cultured PC derived from mouse brain microvessels. A, Cerebral PC stained for α-SMA are green , nuclei stained with 4′,6-diamidino-2-phenylindole (DAPI) are blue . PC (1 × 10 4 ) were fixed on chamber slides with 4% paraformaldehyde in PBS, permeabilized, blocked with 0.3% Triton, 10% normal donkey serum (NDS) in PBS, and incubated with mouse antihuman α-SMA antibody at 10 μg/ml in 1% NDS in PBS. Secondary antibody was goat antimouse fluorescein isothiocyanate-conjugated IgG (1:200). After incubation and washing, slides were counterstained with DAPI (10 μg/ml) in mounting media. B, Transcripts of CA VA and VB in the PC and the brain. RNA isolated from PC was reversed transcribed and PCR amplified by mitochondrial CA VA-specific ( top ) and CA VB-specific ( bottom ) primers. Mouse brain RNA was used as a positive control. C, Mitochondrial CA VA and VB polypeptides in the PC and the brain. Proteins (50 μg) isolated from the PC were separated on polyacrylamide gels and probed with antimouse CA VA or VB antibody at 1:3000 dilution. Secondary antibody was goat antirabbit horseradish peroxidase (1:5000)-conjugated IgG. Proteins from the mouse brain were used as a positive control.

    Journal: Endocrinology

    Article Title: Topiramate Treatment Protects Blood-Brain Barrier Pericytes from Hyperglycemia-Induced Oxidative Damage in Diabetic Mice

    doi: 10.1210/en.2011-1638

    Figure Lengend Snippet: Mitochondrial CA (mCA) in the primary cultured PC derived from mouse brain microvessels. A, Cerebral PC stained for α-SMA are green , nuclei stained with 4′,6-diamidino-2-phenylindole (DAPI) are blue . PC (1 × 10 4 ) were fixed on chamber slides with 4% paraformaldehyde in PBS, permeabilized, blocked with 0.3% Triton, 10% normal donkey serum (NDS) in PBS, and incubated with mouse antihuman α-SMA antibody at 10 μg/ml in 1% NDS in PBS. Secondary antibody was goat antimouse fluorescein isothiocyanate-conjugated IgG (1:200). After incubation and washing, slides were counterstained with DAPI (10 μg/ml) in mounting media. B, Transcripts of CA VA and VB in the PC and the brain. RNA isolated from PC was reversed transcribed and PCR amplified by mitochondrial CA VA-specific ( top ) and CA VB-specific ( bottom ) primers. Mouse brain RNA was used as a positive control. C, Mitochondrial CA VA and VB polypeptides in the PC and the brain. Proteins (50 μg) isolated from the PC were separated on polyacrylamide gels and probed with antimouse CA VA or VB antibody at 1:3000 dilution. Secondary antibody was goat antirabbit horseradish peroxidase (1:5000)-conjugated IgG. Proteins from the mouse brain were used as a positive control.

    Article Snippet: The antihuman FVIII antibody (MAB3440) and goat antimouse IgG conjugated with fluorescein isothiocyanate or Rhodamine Red were from Millipore (Billerica, MA).

    Techniques: Cell Culture, Derivative Assay, Staining, Incubation, Isolation, Polymerase Chain Reaction, Amplification, Positive Control

    Analysis of IgG1 (A) and IgG2a (B) antibody responses of BALB/c mice to the recombinant BFR and P39 antigens and to B. abortus 544 extract. Mice were immunized three times with BFR or P39 in different ODN combinations. Sera from four mice per group collected 3 weeks after the last immunization were assayed individually by ELISA. Antibody levels are expressed as mean titer.

    Journal: Infection and Immunity

    Article Title: Protection of BALB/c Mice against Brucella abortus 544 Challenge by Vaccination with Bacterioferritin or P39 Recombinant Proteins with CpG Oligodeoxynucleotides as Adjuvant

    doi: 10.1128/IAI.69.8.4816-4822.2001

    Figure Lengend Snippet: Analysis of IgG1 (A) and IgG2a (B) antibody responses of BALB/c mice to the recombinant BFR and P39 antigens and to B. abortus 544 extract. Mice were immunized three times with BFR or P39 in different ODN combinations. Sera from four mice per group collected 3 weeks after the last immunization were assayed individually by ELISA. Antibody levels are expressed as mean titer.

    Article Snippet: After five washes with washing buffer (PBS–0.05% Tween 20), biotinylated goat anti-mouse IgG1 or anti-mouse IgG2a antibodies (Amersham) were added at an optimal dilution for 1 h at RT.

    Techniques: Mouse Assay, Recombinant, Enzyme-linked Immunosorbent Assay

    Dye-labeled IgG can be used as a probe to detect dilute protein in solution. Strips of PVDF were submerged in solutions of varying concentrations of human serum albumin in PBS buffer. Albumin was then probed for using mouse antihuman albumin, followed by D0 dimer-labeled goat antimouse IgG. The PVDF strips were exposed to PROTOGOLD and LI silver stain solutions, and analyzed by Raman spectroscopy at 532 nm. The resulting spectra were baseline corrected and vector normalized to equivalent scales using OPUS 7.5 software.

    Journal: ACS Omega

    Article Title: Multimeric Rhodamine Dye-Induced Aggregation of Silver Nanoparticles for Surface-Enhanced Raman Scattering

    doi: 10.1021/acsomega.8b02970

    Figure Lengend Snippet: Dye-labeled IgG can be used as a probe to detect dilute protein in solution. Strips of PVDF were submerged in solutions of varying concentrations of human serum albumin in PBS buffer. Albumin was then probed for using mouse antihuman albumin, followed by D0 dimer-labeled goat antimouse IgG. The PVDF strips were exposed to PROTOGOLD and LI silver stain solutions, and analyzed by Raman spectroscopy at 532 nm. The resulting spectra were baseline corrected and vector normalized to equivalent scales using OPUS 7.5 software.

    Article Snippet: One hundred microliters of goat antimouse IgG (2.12 mg/mL; Abcam, Plc.) was transferred to an Amicron Ultra 0.5 mL spin tube (MilliporeSigma), followed by 300 μL of 50 mM NaHCO3 (pH 9.0).

    Techniques: Labeling, Silver Staining, Raman Spectroscopy, Plasmid Preparation, Software

    (a) Schematic diagram of the enzyme-linked immunosorbent assay (ELISA) performed on the PONB nanofiber meshes using streptavidin bridges to connect the biotinylated surface to a biotinylated antimouse IgG. A generic mouse IgG was used as the target molecule,

    Journal: Analytical chemistry

    Article Title: Functionalized Nanofiber Meshes Enhance Immunosorbent Assays

    doi: 10.1021/acs.analchem.5b03386

    Figure Lengend Snippet: (a) Schematic diagram of the enzyme-linked immunosorbent assay (ELISA) performed on the PONB nanofiber meshes using streptavidin bridges to connect the biotinylated surface to a biotinylated antimouse IgG. A generic mouse IgG was used as the target molecule,

    Article Snippet: A generic ELISA assay was performed on the electrospun mesh substrates and a streptavidin coated 96-well plate (Pierce, cat. no. 15125) through the sequential addition of the following solutions (each 100 µL): (1) 500 nM streptavidin (Sigma, S3762) (18 h); (2) 3.125 nM antimouse IgG from goat (abcam, ab6788) (2 h); (3) varying concentrations of mouse IgG (abcam, ab37355) ranging from 0 to 250 pM (0 to 32 ng/mL) (2 h); (4) 2 nM antimouse IgG functionalized with the enzyme horseradish peroxidase (HRP) (abcam, ab6789) (2 h); (5) a solution of 3,3′,5,5′-tetramethylbenzidine (TMB) (abcam, ab171522); and (6) a 450 nm stop solution for TMB substrates (abcam, ab171529).

    Techniques: Enzyme-linked Immunosorbent Assay

    Binding and blocking of BNeV VLPs to synthetic HBGAs. (A) Ninety-six-well plates were coated with 50 μg/ml BNeV VLPs, GST-tagged-VP8* protein and GST-tagged P particles (10 μg/ml), supernatant of wild-type baculovirus-infected Sf9 cells lysate, and GST and then incubated with each of the synthetic HBGAs (10 μg/ml). The binding of each HBGA to target viral proteins and the control was determined by addition of horseradish peroxidase-conjugated streptavidin as described in Materials and Methods. (B) α1,2-Linked fucose, α1,3/4-linked fucose, αGal, and GalNAc epitopes were removed from each synthetic HBGA coated in each well using the corresponding enzyme. After incubation of BNeV VLPs at 50 μg/ml, the binding of BNeV VLPs was determined using hyperimmune serum against BNeV capsid protein, followed by addition of horseradish peroxidase-conjugated goat anti-rabbit IgG antibody. The signal intensities for graphs in panels A and B were visualized using TMB at 450 nm in three independent experiments. Error bars indicate SD from triplicate samples.

    Journal: Journal of Virology

    Article Title: Bovine Nebovirus Interacts with a Wide Spectrum of Histo-Blood Group Antigens

    doi: 10.1128/JVI.02160-17

    Figure Lengend Snippet: Binding and blocking of BNeV VLPs to synthetic HBGAs. (A) Ninety-six-well plates were coated with 50 μg/ml BNeV VLPs, GST-tagged-VP8* protein and GST-tagged P particles (10 μg/ml), supernatant of wild-type baculovirus-infected Sf9 cells lysate, and GST and then incubated with each of the synthetic HBGAs (10 μg/ml). The binding of each HBGA to target viral proteins and the control was determined by addition of horseradish peroxidase-conjugated streptavidin as described in Materials and Methods. (B) α1,2-Linked fucose, α1,3/4-linked fucose, αGal, and GalNAc epitopes were removed from each synthetic HBGA coated in each well using the corresponding enzyme. After incubation of BNeV VLPs at 50 μg/ml, the binding of BNeV VLPs was determined using hyperimmune serum against BNeV capsid protein, followed by addition of horseradish peroxidase-conjugated goat anti-rabbit IgG antibody. The signal intensities for graphs in panels A and B were visualized using TMB at 450 nm in three independent experiments. Error bars indicate SD from triplicate samples.

    Article Snippet: Horseradish peroxidase (HRP)-conjugated streptavidin and HRP-conjugated goat anti-rabbit immunoglobulin G (IgG) and anti-mouse IgG antibodies were obtained from Jackson ImmunoResearch Laboratories or Dako.

    Techniques: Binding Assay, Blocking Assay, Infection, Incubation

    Expression of TCI gene in 293T cells. Transfected cells were incubated with Mabs against the HIV‐1 p24 protein and HRP‐conjugated rabbit anti‐mouse IgG; complex was visualized by staining with a substrate solution containing 3.3′‐diaminobenzidine tetrahydrochloride: (A) 293T cells transfected with pcDNA‐TCI and (B) 293T cells transfected with pcDNA3.1. Cells were visualized by microscopy with an Axioskop 2 (Carl Zeiss).

    Journal: Microbial biotechnology

    Article Title: Attenuated Salmonella enteritidis E23 as a vehicle for the rectal delivery of DNA vaccine coding for HIV-1 polyepitope CTL immunogen

    doi: 10.1111/j.1751-7915.2011.00291.x

    Figure Lengend Snippet: Expression of TCI gene in 293T cells. Transfected cells were incubated with Mabs against the HIV‐1 p24 protein and HRP‐conjugated rabbit anti‐mouse IgG; complex was visualized by staining with a substrate solution containing 3.3′‐diaminobenzidine tetrahydrochloride: (A) 293T cells transfected with pcDNA‐TCI and (B) 293T cells transfected with pcDNA3.1. Cells were visualized by microscopy with an Axioskop 2 (Carl Zeiss).

    Article Snippet: The anti‐mouse IgG antibodies conjugated to horseradish peroxidase (Sigma) were used as secondary antibodies and 3,3′,5,5′‐tetramethylbenzidine (TMB) as a substrate.

    Techniques: Expressing, Transfection, Incubation, Staining, Microscopy