Journal: Journal of Immunology Research
Article Title: Stabilized β-Catenin Ameliorates ALPS-Like Symptoms of B6/lpr Mice
Figure Lengend Snippet: β -cat Tg potentiated the apoptosis of T cells of lpr / lpr mice. WT, β -cat Tg , lpr / lpr , and β -cat Tg lpr / lpr female mice were sacrificed at age 8–12 weeks and splenocytes were isolated. The CD4 + T cells obtained from splenocytes were expanded in IL-2, IL-7, and IL-15 for 5 days. The T cell apoptosis then was induced by using different concentrations of anti-CD3 Ab ( μ g/ml). (a) The representative FACS results of T cells apoptosis with anti-CD3 Ab (10 μ g/ml). The cell pellets were stained with anti-CD3-APC, Annexin V, and 7-AAD surface markers, then the flow cytometry was used to analyze the apoptotic of T cells. (b) The representative statistical analysis of the apoptotic percentages of T cells. Data are shown as mean ± SD ( n = 3). ∗ p
Article Snippet: Antibodies and Flow Cytometry Anti-mouse CD3 (clone PC-61) (QuantoBio), anti-mouse TCRβ chain (clone H75-597) (eBioscience, San Diego, CA, USA), anti-mouse CD4 (clone RM4-5) (eBioscience), anti-mouse CD8a (clone 53-6.7) (eBioscience), rat anti-mouse CD44 (clone IM7) (BD Biosciences, Franklin Lakes, NJ, USA), and rat anti-mouse CD62L (MEL-14) (BD Biosciences) fluorochrome-labeled antibodies were used for flow cytometry.
Techniques: Mouse Assay, Isolation, FACS, Staining, Flow Cytometry, Cytometry