anti-mmp-9 ab13458 Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Millipore anti mmp 9
    Stimulation of <t>MMP-9</t> protein expression and inflammatory markers via pMCAO. a The expression levels of both the active (86 kDa) and the inactive (92 kDa) forms of MMP-9 were determined at different times after surgery. b The ischaemic-induced phosphorylation of p38 and CaMKII as well as the expression levels of nNOS and GFAP were evaluated 8 days after surgery. The enhancing effects of pMCAO ( light grey ) were reduced by S1RA administration ( dark grey ). Each bar represents the means ± SEM of the data from three determinations performed using different gels and blots. Groups of 8–10 mice were used for each interval. Immunosignals (average optical density of the pixels within the object area/mm 2 ; Quantity One Software, Bio-Rad, Madrid, Spain) were expressed as the change relative to the sham-operated group (attributed an arbitrary value of 1; white bars ). Asterisk denotes the significant difference from the sham-operated mice, θ from the pMCAO group, all pairwise Holm-Sidak multiple comparison test following ANOVA, p
    Anti Mmp 9, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 321 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mmp 9/product/Millipore
    Average 95 stars, based on 321 article reviews
    Price from $9.99 to $1999.99
    anti mmp 9 - by Bioz Stars, 2020-09
    95/100 stars
      Buy from Supplier

    99
    Abcam anti bcl 2 antibody
    The P2X7R inhibitor suppressed PA-induced inflammation, fibrosis and apoptosis in cardiomyocytes. (A) Cell viability was assessed by the CCK8 assay. H9c2 myocytes were incubated in a medium with A438079 at 0–80 μM for 12 h, and cells without A438079 treatment served as the control. (B) H9c2 myocytes were incubated in a medium with PA at 0–800 μM for 24 h, and cells without PA treatment served as the control. (C) Concentrations of IL-1β in cell culture supernatants were detected by ELISA. (D) Representative Western blot analysis of P2X7R, NLRP3 inflammasome, collagen I, MMP9, TGF-β, caspase-1, caspase-3, Bax, and <t>Bcl-2.</t> (E) The protein semiquantification is shown for P2X7R, NLRP3 and caspase-1. (F) The protein semiquantification is shown for collagen I, MMP9 and TGF-β. (G) The protein semiquantification is shown for caspase-3, Bax, and Bcl-2. (H) The percentages of TUNEL-positive cells are shown. (I) Representative images of TUNEL stained H9c2 myocytes. CON, H9c2 myocytes without any treatment; PA, H9c2 myocytes treated with 200 μM PA for 24 h; PA + A438079, H9c2 myocytes pretreated with A438079 for 12 h, and then treated with 200 μM PA for 24 h. ∗ p
    Anti Bcl 2 Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 1493 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti bcl 2 antibody/product/Abcam
    Average 99 stars, based on 1493 article reviews
    Price from $9.99 to $1999.99
    anti bcl 2 antibody - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Abcam anti superoxide dismutase 1 antibody
    The P2X7R inhibitor suppressed PA-induced inflammation, fibrosis and apoptosis in cardiomyocytes. (A) Cell viability was assessed by the CCK8 assay. H9c2 myocytes were incubated in a medium with A438079 at 0–80 μM for 12 h, and cells without A438079 treatment served as the control. (B) H9c2 myocytes were incubated in a medium with PA at 0–800 μM for 24 h, and cells without PA treatment served as the control. (C) Concentrations of IL-1β in cell culture supernatants were detected by ELISA. (D) Representative Western blot analysis of P2X7R, NLRP3 inflammasome, collagen I, MMP9, TGF-β, caspase-1, caspase-3, Bax, and <t>Bcl-2.</t> (E) The protein semiquantification is shown for P2X7R, NLRP3 and caspase-1. (F) The protein semiquantification is shown for collagen I, MMP9 and TGF-β. (G) The protein semiquantification is shown for caspase-3, Bax, and Bcl-2. (H) The percentages of TUNEL-positive cells are shown. (I) Representative images of TUNEL stained H9c2 myocytes. CON, H9c2 myocytes without any treatment; PA, H9c2 myocytes treated with 200 μM PA for 24 h; PA + A438079, H9c2 myocytes pretreated with A438079 for 12 h, and then treated with 200 μM PA for 24 h. ∗ p
    Anti Superoxide Dismutase 1 Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti superoxide dismutase 1 antibody/product/Abcam
    Average 99 stars, based on 97 article reviews
    Price from $9.99 to $1999.99
    anti superoxide dismutase 1 antibody - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Abcam β actin
    The P2X7R inhibitor suppressed PA-induced inflammation, fibrosis and apoptosis in cardiomyocytes. (A) Cell viability was assessed by the CCK8 assay. H9c2 myocytes were incubated in a medium with A438079 at 0–80 μM for 12 h, and cells without A438079 treatment served as the control. (B) H9c2 myocytes were incubated in a medium with PA at 0–800 μM for 24 h, and cells without PA treatment served as the control. (C) Concentrations of IL-1β in cell culture supernatants were detected by ELISA. (D) Representative Western blot analysis of P2X7R, NLRP3 inflammasome, collagen I, MMP9, TGF-β, caspase-1, caspase-3, Bax, and <t>Bcl-2.</t> (E) The protein semiquantification is shown for P2X7R, NLRP3 and caspase-1. (F) The protein semiquantification is shown for collagen I, MMP9 and TGF-β. (G) The protein semiquantification is shown for caspase-3, Bax, and Bcl-2. (H) The percentages of TUNEL-positive cells are shown. (I) Representative images of TUNEL stained H9c2 myocytes. CON, H9c2 myocytes without any treatment; PA, H9c2 myocytes treated with 200 μM PA for 24 h; PA + A438079, H9c2 myocytes pretreated with A438079 for 12 h, and then treated with 200 μM PA for 24 h. ∗ p
    β Actin, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 25356 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/β actin/product/Abcam
    Average 99 stars, based on 25356 article reviews
    Price from $9.99 to $1999.99
    β actin - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    akt  (Abcam)
    99
    Abcam akt
    Analysis of TNF-α-induced <t>ERK/ATK</t> signaling pathway in hPMCs isolated from patients with MTB. Expression levels of (A) ERK and (B) <t>Akt</t> were evaluated in hPMCs isolated from patients with MTB. Phosphorylation levels of (C) ERK and (D) Akt in hPMCs isolated from patients with MTB. (E) Expression levels and (F) phosphorylation levels of ERK and Akt were evaluated in si-TNF-α-treated hPMCs. Expression levels of (G) MMP-1 and (H) MMP-9 were evaluated in si-TNF-α-treated hPMCs. Data are expressed as the mean ± standard deviation of three independent experiments. **P
    Akt, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 2501 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/akt/product/Abcam
    Average 99 stars, based on 2501 article reviews
    Price from $9.99 to $1999.99
    akt - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Abcam anti pan akt phospho t308 antibody
    Analysis of TNF-α-induced <t>ERK/ATK</t> signaling pathway in hPMCs isolated from patients with MTB. Expression levels of (A) ERK and (B) <t>Akt</t> were evaluated in hPMCs isolated from patients with MTB. Phosphorylation levels of (C) ERK and (D) Akt in hPMCs isolated from patients with MTB. (E) Expression levels and (F) phosphorylation levels of ERK and Akt were evaluated in si-TNF-α-treated hPMCs. Expression levels of (G) MMP-1 and (H) MMP-9 were evaluated in si-TNF-α-treated hPMCs. Data are expressed as the mean ± standard deviation of three independent experiments. **P
    Anti Pan Akt Phospho T308 Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 205 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti pan akt phospho t308 antibody/product/Abcam
    Average 99 stars, based on 205 article reviews
    Price from $9.99 to $1999.99
    anti pan akt phospho t308 antibody - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Abcam anti mmp9 antibody
    Expression levels of TNF-α and MMPs in patients with MTB. Serum levels of (A) TNF-α, (B) MMP-1 and (C) <t>MMP-9</t> were evaluated in patients with MTB. Protein expression levels of (D) TNF-α, (E) MMP-1 and (F) MMP-9 in human pleural mesothelial cells isolated from patients with MTB. Data are expressed as the mean ± standard deviation of three independent experiments. **P
    Anti Mmp9 Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 607 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mmp9 antibody/product/Abcam
    Average 99 stars, based on 607 article reviews
    Price from $9.99 to $1999.99
    anti mmp9 antibody - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    92
    OriGene goat anti rabbit igg mab
    Expression levels of TNF-α and MMPs in patients with MTB. Serum levels of (A) TNF-α, (B) MMP-1 and (C) <t>MMP-9</t> were evaluated in patients with MTB. Protein expression levels of (D) TNF-α, (E) MMP-1 and (F) MMP-9 in human pleural mesothelial cells isolated from patients with MTB. Data are expressed as the mean ± standard deviation of three independent experiments. **P
    Goat Anti Rabbit Igg Mab, supplied by OriGene, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti rabbit igg mab/product/OriGene
    Average 92 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    goat anti rabbit igg mab - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    95
    Abcam anti mmp 1
    Expression levels of TNF-α and MMPs in patients with MTB. Serum levels of (A) TNF-α, (B) <t>MMP-1</t> and (C) MMP-9 were evaluated in patients with MTB. Protein expression levels of (D) TNF-α, (E) MMP-1 and (F) MMP-9 in human pleural mesothelial cells isolated from patients with MTB. Data are expressed as the mean ± standard deviation of three independent experiments. **P
    Anti Mmp 1, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 79 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti mmp 1/product/Abcam
    Average 95 stars, based on 79 article reviews
    Price from $9.99 to $1999.99
    anti mmp 1 - by Bioz Stars, 2020-09
    95/100 stars
      Buy from Supplier

    erk  (Abcam)
    99
    Abcam erk
    Expression levels of TNF-α and MMPs in patients with MTB. Serum levels of (A) TNF-α, (B) <t>MMP-1</t> and (C) MMP-9 were evaluated in patients with MTB. Protein expression levels of (D) TNF-α, (E) MMP-1 and (F) MMP-9 in human pleural mesothelial cells isolated from patients with MTB. Data are expressed as the mean ± standard deviation of three independent experiments. **P
    Erk, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 616 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/erk/product/Abcam
    Average 99 stars, based on 616 article reviews
    Price from $9.99 to $1999.99
    erk - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    92
    Abcam rabbit anti mouse nuclear factor κb nf κb
    Expression levels of TNF-α and MMPs in patients with MTB. Serum levels of (A) TNF-α, (B) <t>MMP-1</t> and (C) MMP-9 were evaluated in patients with MTB. Protein expression levels of (D) TNF-α, (E) MMP-1 and (F) MMP-9 in human pleural mesothelial cells isolated from patients with MTB. Data are expressed as the mean ± standard deviation of three independent experiments. **P
    Rabbit Anti Mouse Nuclear Factor κb Nf κb, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti mouse nuclear factor κb nf κb/product/Abcam
    Average 92 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    rabbit anti mouse nuclear factor κb nf κb - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    99
    Abcam rabbit anti mouse interleukin 1 β il 1β polyclonal antibody polyclonal antibody 1 1000
    Expression levels of TNF-α and MMPs in patients with MTB. Serum levels of (A) TNF-α, (B) <t>MMP-1</t> and (C) MMP-9 were evaluated in patients with MTB. Protein expression levels of (D) TNF-α, (E) MMP-1 and (F) MMP-9 in human pleural mesothelial cells isolated from patients with MTB. Data are expressed as the mean ± standard deviation of three independent experiments. **P
    Rabbit Anti Mouse Interleukin 1 β Il 1β Polyclonal Antibody Polyclonal Antibody 1 1000, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti mouse interleukin 1 β il 1β polyclonal antibody polyclonal antibody 1 1000/product/Abcam
    Average 99 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    rabbit anti mouse interleukin 1 β il 1β polyclonal antibody polyclonal antibody 1 1000 - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Abcam rabbit anti mouse tumor necrosis factor α tnf α polyclonal antibody polyclonal antibody 1 1000
    Expression levels of TNF-α and MMPs in patients with MTB. Serum levels of (A) TNF-α, (B) <t>MMP-1</t> and (C) MMP-9 were evaluated in patients with MTB. Protein expression levels of (D) TNF-α, (E) MMP-1 and (F) MMP-9 in human pleural mesothelial cells isolated from patients with MTB. Data are expressed as the mean ± standard deviation of three independent experiments. **P
    Rabbit Anti Mouse Tumor Necrosis Factor α Tnf α Polyclonal Antibody Polyclonal Antibody 1 1000, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti mouse tumor necrosis factor α tnf α polyclonal antibody polyclonal antibody 1 1000/product/Abcam
    Average 99 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    rabbit anti mouse tumor necrosis factor α tnf α polyclonal antibody polyclonal antibody 1 1000 - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Abcam rabbit anti mouse superoxide dismutase 1 sod1 polyclonal antibody polyclonal antibody 1 1000
    Expression levels of TNF-α and MMPs in patients with MTB. Serum levels of (A) TNF-α, (B) <t>MMP-1</t> and (C) MMP-9 were evaluated in patients with MTB. Protein expression levels of (D) TNF-α, (E) MMP-1 and (F) MMP-9 in human pleural mesothelial cells isolated from patients with MTB. Data are expressed as the mean ± standard deviation of three independent experiments. **P
    Rabbit Anti Mouse Superoxide Dismutase 1 Sod1 Polyclonal Antibody Polyclonal Antibody 1 1000, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti mouse superoxide dismutase 1 sod1 polyclonal antibody polyclonal antibody 1 1000/product/Abcam
    Average 99 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    rabbit anti mouse superoxide dismutase 1 sod1 polyclonal antibody polyclonal antibody 1 1000 - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    95
    Abcam rabbit anti mouse glutamate cysteine ligase catalytic subunit gclc polyclonal antibody
    Expression levels of TNF-α and MMPs in patients with MTB. Serum levels of (A) TNF-α, (B) <t>MMP-1</t> and (C) MMP-9 were evaluated in patients with MTB. Protein expression levels of (D) TNF-α, (E) MMP-1 and (F) MMP-9 in human pleural mesothelial cells isolated from patients with MTB. Data are expressed as the mean ± standard deviation of three independent experiments. **P
    Rabbit Anti Mouse Glutamate Cysteine Ligase Catalytic Subunit Gclc Polyclonal Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti mouse glutamate cysteine ligase catalytic subunit gclc polyclonal antibody/product/Abcam
    Average 95 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    rabbit anti mouse glutamate cysteine ligase catalytic subunit gclc polyclonal antibody - by Bioz Stars, 2020-09
    95/100 stars
      Buy from Supplier

    92
    Abcam rat anti human anti tnf α
    Expression levels of <t>TNF-α</t> and MMPs in patients with MTB. Serum levels of (A) TNF-α, (B) MMP-1 and (C) MMP-9 were evaluated in patients with MTB. Protein expression levels of (D) TNF-α, (E) MMP-1 and (F) MMP-9 in human pleural mesothelial cells isolated from patients with MTB. Data are expressed as the mean ± standard deviation of three independent experiments. **P
    Rat Anti Human Anti Tnf α, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rat anti human anti tnf α/product/Abcam
    Average 92 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    rat anti human anti tnf α - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    99
    Abcam p65 polyclonal antibody
    Expression levels of <t>TNF-α</t> and MMPs in patients with MTB. Serum levels of (A) TNF-α, (B) MMP-1 and (C) MMP-9 were evaluated in patients with MTB. Protein expression levels of (D) TNF-α, (E) MMP-1 and (F) MMP-9 in human pleural mesothelial cells isolated from patients with MTB. Data are expressed as the mean ± standard deviation of three independent experiments. **P
    P65 Polyclonal Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/p65 polyclonal antibody/product/Abcam
    Average 99 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
    p65 polyclonal antibody - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    99
    Abcam nfe2l2 polyclonal antibody
    Expression levels of <t>TNF-α</t> and MMPs in patients with MTB. Serum levels of (A) TNF-α, (B) MMP-1 and (C) MMP-9 were evaluated in patients with MTB. Protein expression levels of (D) TNF-α, (E) MMP-1 and (F) MMP-9 in human pleural mesothelial cells isolated from patients with MTB. Data are expressed as the mean ± standard deviation of three independent experiments. **P
    Nfe2l2 Polyclonal Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nfe2l2 polyclonal antibody/product/Abcam
    Average 99 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    nfe2l2 polyclonal antibody - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    pakt  (Abcam)
    97
    Abcam pakt
    Expression levels of <t>TNF-α</t> and MMPs in patients with MTB. Serum levels of (A) TNF-α, (B) MMP-1 and (C) MMP-9 were evaluated in patients with MTB. Protein expression levels of (D) TNF-α, (E) MMP-1 and (F) MMP-9 in human pleural mesothelial cells isolated from patients with MTB. Data are expressed as the mean ± standard deviation of three independent experiments. **P
    Pakt, supplied by Abcam, used in various techniques. Bioz Stars score: 97/100, based on 305 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pakt/product/Abcam
    Average 97 stars, based on 305 article reviews
    Price from $9.99 to $1999.99
    pakt - by Bioz Stars, 2020-09
    97/100 stars
      Buy from Supplier

    Image Search Results


    Stimulation of MMP-9 protein expression and inflammatory markers via pMCAO. a The expression levels of both the active (86 kDa) and the inactive (92 kDa) forms of MMP-9 were determined at different times after surgery. b The ischaemic-induced phosphorylation of p38 and CaMKII as well as the expression levels of nNOS and GFAP were evaluated 8 days after surgery. The enhancing effects of pMCAO ( light grey ) were reduced by S1RA administration ( dark grey ). Each bar represents the means ± SEM of the data from three determinations performed using different gels and blots. Groups of 8–10 mice were used for each interval. Immunosignals (average optical density of the pixels within the object area/mm 2 ; Quantity One Software, Bio-Rad, Madrid, Spain) were expressed as the change relative to the sham-operated group (attributed an arbitrary value of 1; white bars ). Asterisk denotes the significant difference from the sham-operated mice, θ from the pMCAO group, all pairwise Holm-Sidak multiple comparison test following ANOVA, p

    Journal: Molecular Neurobiology

    Article Title: The Sigma-1 Receptor Antagonist, S1RA, Reduces Stroke Damage, Ameliorates Post-Stroke Neurological Deficits and Suppresses the Overexpression of MMP-9

    doi: 10.1007/s12035-017-0697-x

    Figure Lengend Snippet: Stimulation of MMP-9 protein expression and inflammatory markers via pMCAO. a The expression levels of both the active (86 kDa) and the inactive (92 kDa) forms of MMP-9 were determined at different times after surgery. b The ischaemic-induced phosphorylation of p38 and CaMKII as well as the expression levels of nNOS and GFAP were evaluated 8 days after surgery. The enhancing effects of pMCAO ( light grey ) were reduced by S1RA administration ( dark grey ). Each bar represents the means ± SEM of the data from three determinations performed using different gels and blots. Groups of 8–10 mice were used for each interval. Immunosignals (average optical density of the pixels within the object area/mm 2 ; Quantity One Software, Bio-Rad, Madrid, Spain) were expressed as the change relative to the sham-operated group (attributed an arbitrary value of 1; white bars ). Asterisk denotes the significant difference from the sham-operated mice, θ from the pMCAO group, all pairwise Holm-Sidak multiple comparison test following ANOVA, p

    Article Snippet: The primary antibodies were as follows: anti-MMP-9 (1:1000, Millipore AB13458), PhosphoDetect anti-p38 MAP kinase which specifically recognises the dual-phosphorylated (on residues Thr180 and Tyr182) active form of the enzyme (1:1000, Millipore 506119), Phospho-CaMKII (Thr286) antibody (1:1000, Cell Signalling #12716), anti-nNOS (1:1000) and anti-GFAP (1:1000).

    Techniques: Expressing, Mouse Assay, Software

    The P2X7R inhibitor suppressed PA-induced inflammation, fibrosis and apoptosis in cardiomyocytes. (A) Cell viability was assessed by the CCK8 assay. H9c2 myocytes were incubated in a medium with A438079 at 0–80 μM for 12 h, and cells without A438079 treatment served as the control. (B) H9c2 myocytes were incubated in a medium with PA at 0–800 μM for 24 h, and cells without PA treatment served as the control. (C) Concentrations of IL-1β in cell culture supernatants were detected by ELISA. (D) Representative Western blot analysis of P2X7R, NLRP3 inflammasome, collagen I, MMP9, TGF-β, caspase-1, caspase-3, Bax, and Bcl-2. (E) The protein semiquantification is shown for P2X7R, NLRP3 and caspase-1. (F) The protein semiquantification is shown for collagen I, MMP9 and TGF-β. (G) The protein semiquantification is shown for caspase-3, Bax, and Bcl-2. (H) The percentages of TUNEL-positive cells are shown. (I) Representative images of TUNEL stained H9c2 myocytes. CON, H9c2 myocytes without any treatment; PA, H9c2 myocytes treated with 200 μM PA for 24 h; PA + A438079, H9c2 myocytes pretreated with A438079 for 12 h, and then treated with 200 μM PA for 24 h. ∗ p

    Journal: Frontiers in Physiology

    Article Title: Aerobic Exercise Ameliorates Myocardial Inflammation, Fibrosis and Apoptosis in High-Fat-Diet Rats by Inhibiting P2X7 Purinergic Receptors

    doi: 10.3389/fphys.2019.01286

    Figure Lengend Snippet: The P2X7R inhibitor suppressed PA-induced inflammation, fibrosis and apoptosis in cardiomyocytes. (A) Cell viability was assessed by the CCK8 assay. H9c2 myocytes were incubated in a medium with A438079 at 0–80 μM for 12 h, and cells without A438079 treatment served as the control. (B) H9c2 myocytes were incubated in a medium with PA at 0–800 μM for 24 h, and cells without PA treatment served as the control. (C) Concentrations of IL-1β in cell culture supernatants were detected by ELISA. (D) Representative Western blot analysis of P2X7R, NLRP3 inflammasome, collagen I, MMP9, TGF-β, caspase-1, caspase-3, Bax, and Bcl-2. (E) The protein semiquantification is shown for P2X7R, NLRP3 and caspase-1. (F) The protein semiquantification is shown for collagen I, MMP9 and TGF-β. (G) The protein semiquantification is shown for caspase-3, Bax, and Bcl-2. (H) The percentages of TUNEL-positive cells are shown. (I) Representative images of TUNEL stained H9c2 myocytes. CON, H9c2 myocytes without any treatment; PA, H9c2 myocytes treated with 200 μM PA for 24 h; PA + A438079, H9c2 myocytes pretreated with A438079 for 12 h, and then treated with 200 μM PA for 24 h. ∗ p

    Article Snippet: Collagen 1 (ab34710), matrix metalloproteinases (MMP9) (ab38898) and Bcl-2 (ab196495) antibodies were obtained from Abcam (Cambridge, United Kingdom).

    Techniques: CCK-8 Assay, Incubation, Cell Culture, Enzyme-linked Immunosorbent Assay, Western Blot, TUNEL Assay, Staining

    Regular aerobic exercise inhibited myocardial apoptosis in HFD rats. (A) Representative TUNEL stained images of rat left ventricles. (B) The percentage of TUNEL-positive cells are shown. (C) Representative Western blot analysis of caspase-3, Bcl-2, and Bax. (D) The mRNA levels and the protein semiquantification are shown for caspase-3. (E) The mRNA levels and the protein semiquantification are shown for Bcl-2. (F) The mRNA levels and the protein semiquantification are shown for Bax. ∗ p

    Journal: Frontiers in Physiology

    Article Title: Aerobic Exercise Ameliorates Myocardial Inflammation, Fibrosis and Apoptosis in High-Fat-Diet Rats by Inhibiting P2X7 Purinergic Receptors

    doi: 10.3389/fphys.2019.01286

    Figure Lengend Snippet: Regular aerobic exercise inhibited myocardial apoptosis in HFD rats. (A) Representative TUNEL stained images of rat left ventricles. (B) The percentage of TUNEL-positive cells are shown. (C) Representative Western blot analysis of caspase-3, Bcl-2, and Bax. (D) The mRNA levels and the protein semiquantification are shown for caspase-3. (E) The mRNA levels and the protein semiquantification are shown for Bcl-2. (F) The mRNA levels and the protein semiquantification are shown for Bax. ∗ p

    Article Snippet: Collagen 1 (ab34710), matrix metalloproteinases (MMP9) (ab38898) and Bcl-2 (ab196495) antibodies were obtained from Abcam (Cambridge, United Kingdom).

    Techniques: TUNEL Assay, Staining, Western Blot

    The expression of p-AMPK, Bcl-2 and Bax protein. (A) The protein band; (B) the expression level of p-AMPK; (C) the expression level of Bcl-2/Bax. The serum levels of p-AMPK and Bcl-2/Bax in the intervention group and the craniotomy group were significantly higher than those in control group, **P

    Journal: Experimental and Therapeutic Medicine

    Article Title: The effect of intravascular interventional embolization and craniotomy on MMP-2, MMP-9 and caspase3 in serum of intracranial aneurysm patients

    doi: 10.3892/etm.2018.6740

    Figure Lengend Snippet: The expression of p-AMPK, Bcl-2 and Bax protein. (A) The protein band; (B) the expression level of p-AMPK; (C) the expression level of Bcl-2/Bax. The serum levels of p-AMPK and Bcl-2/Bax in the intervention group and the craniotomy group were significantly higher than those in control group, **P

    Article Snippet: The wet transfer method was performed to transfer the protein to a PVDF membrane, and closed for 1 h. The purpose band was cut and incubated with the corresponding primary rabbit anti-human polyclonal antibodies: MMP-2 (dilution, 1:1,000; cat. no. ab37150; Abcam, Cambridge, UK), MMP-9 (dilution, 1:1,000; cat. no. ab73734; Abcam), caspase3 (dilution, 1:1,000; cat. no. ab13847; Abcam), p-AMPK (dilution, 1:1,000; cat. no. ab3760; Abcam), Bcl-2 (dilution, 1:1,000; cat. no. ab196495; Abcam), Bax (dilution, 1:1,000; cat. no. ab53154; Abcam) and β-actin (dilution, 1:1,000; cat. no. ab8227; Abcam) overnight.

    Techniques: Expressing

    The effect of TUG1 on the expression of proteins involved in cell proliferation, cell cycle, and cell apoptosis via regulating the AEG1 level. ( A ) Upregulation of TUG1 enhanced Bcl-2, MMP-9, and cyclin D1 expression, and depressed the expression of cleaved caspase 3. Sh-AEG1 plasmids reversed the function of pcDNA-TUG1 in these protein expressions. ( B ) Downregulation of TUG1 inhibited the expression of Bcl-2, MMP-9, and cyclin D1, and elevated the level of cleaved caspase 3, but the function of sh-TUG1 was abrogated by the cotransfection of pcDNA-AEG1 in A375 cells. * p

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Long Noncoding RNA Taurine-Upregulated Gene1 (TUG1) Promotes Tumor Growth and Metastasis Through TUG1/Mir-129-5p/Astrocyte-Elevated Gene-1 (AEG-1) Axis in Malignant Melanoma

    doi: 10.12659/MSM.906616

    Figure Lengend Snippet: The effect of TUG1 on the expression of proteins involved in cell proliferation, cell cycle, and cell apoptosis via regulating the AEG1 level. ( A ) Upregulation of TUG1 enhanced Bcl-2, MMP-9, and cyclin D1 expression, and depressed the expression of cleaved caspase 3. Sh-AEG1 plasmids reversed the function of pcDNA-TUG1 in these protein expressions. ( B ) Downregulation of TUG1 inhibited the expression of Bcl-2, MMP-9, and cyclin D1, and elevated the level of cleaved caspase 3, but the function of sh-TUG1 was abrogated by the cotransfection of pcDNA-AEG1 in A375 cells. * p

    Article Snippet: The following antibodies as primary antibody were applied to detect the targeting proteins expression: anti-AEG1 (Rabbit 1: 500; MyBioSource, USA), anti-Bcl-2 (1: 1000; Abcam, UK), anti-MMP-9 (1: 1000; Abcam, UK), anti-cleaved caspase 3 (1: 500; Abcam, UK), anti-cyclin D1 (1: 2000; Abcam, UK) and anti-β-actin (1: 2000; Abcam, UK).

    Techniques: Expressing, Cotransfection

    miR-378a-3p suppresses the expression of associated proteins. Expression levels of caspase-3, caspase-9, Bcl-2, Bax were analyzed in esophageal squamous cell carcinoma cell lines. For each experiment, n=3 ( * P

    Journal: International Journal of Molecular Medicine

    Article Title: miR-378a-3p exerts tumor suppressive function on the tumorigenesis of esophageal squamous cell carcinoma by targeting Rab10

    doi: 10.3892/ijmm.2018.3639

    Figure Lengend Snippet: miR-378a-3p suppresses the expression of associated proteins. Expression levels of caspase-3, caspase-9, Bcl-2, Bax were analyzed in esophageal squamous cell carcinoma cell lines. For each experiment, n=3 ( * P

    Article Snippet: The antibodies used were as follows: Anti-human GAPDH antibody (cat no. ab9485; 1:5,000, Abcam, Cambridge, UK), anti-human B-cell lymphoma (Bcl-2) antibody (cat. no. ab196495; 1:2,000, Abcam), anti-human Bcl-2-associated X protein (Bax) antibody (cat. no. ab32503; 1:2,000, Abcam), anti-human caspase-3 antibody (cat. no. ab13847; 1:2,000, Abcam), anti-human caspase-9 antibody (cat. no. ab202068, 1:2,000, Abcam), anti-human MMP-2 antibody (cat. no. ab37150; 1:2,000, Abcam), anti-human MMP-9 antibody (cat. no. ab73734; 1:2,000, Abcam,).

    Techniques: Expressing

    Analysis of TNF-α-induced ERK/ATK signaling pathway in hPMCs isolated from patients with MTB. Expression levels of (A) ERK and (B) Akt were evaluated in hPMCs isolated from patients with MTB. Phosphorylation levels of (C) ERK and (D) Akt in hPMCs isolated from patients with MTB. (E) Expression levels and (F) phosphorylation levels of ERK and Akt were evaluated in si-TNF-α-treated hPMCs. Expression levels of (G) MMP-1 and (H) MMP-9 were evaluated in si-TNF-α-treated hPMCs. Data are expressed as the mean ± standard deviation of three independent experiments. **P

    Journal: Experimental and Therapeutic Medicine

    Article Title: Inflammation responses in patients with pulmonary tuberculosis in an intensive care unit

    doi: 10.3892/etm.2018.5775

    Figure Lengend Snippet: Analysis of TNF-α-induced ERK/ATK signaling pathway in hPMCs isolated from patients with MTB. Expression levels of (A) ERK and (B) Akt were evaluated in hPMCs isolated from patients with MTB. Phosphorylation levels of (C) ERK and (D) Akt in hPMCs isolated from patients with MTB. (E) Expression levels and (F) phosphorylation levels of ERK and Akt were evaluated in si-TNF-α-treated hPMCs. Expression levels of (G) MMP-1 and (H) MMP-9 were evaluated in si-TNF-α-treated hPMCs. Data are expressed as the mean ± standard deviation of three independent experiments. **P

    Article Snippet: The primary antibodies of rat anti-human anti-TNF-α (1:1,000 dilution, ab667), anti-MMP-1 (1:1,000 dilution, ab137332), anti-MMP-9 (1:1,000 dilution, ab73734), high mobility group box-1 protein (HMGB1, 1:1,000 dilution, ab18256), ERK (1:2,000 dilution, ab196883, Abcam), pERK (1:2,000 dilution, ab214362, Abcam), AKT (1:1,000 dilution, ab8805, Abcam), pAKT (1:1,000 dilution, ab133458, Abcam), and β-actin (1:1,000 dilution, ab8226; all Abcam, Cambridge, UK) were used to incubate with the membranes for 120 min at 37°C.

    Techniques: Isolation, Expressing, Standard Deviation

    Expression levels of TNF-α and MMPs in patients with MTB. Serum levels of (A) TNF-α, (B) MMP-1 and (C) MMP-9 were evaluated in patients with MTB. Protein expression levels of (D) TNF-α, (E) MMP-1 and (F) MMP-9 in human pleural mesothelial cells isolated from patients with MTB. Data are expressed as the mean ± standard deviation of three independent experiments. **P

    Journal: Experimental and Therapeutic Medicine

    Article Title: Inflammation responses in patients with pulmonary tuberculosis in an intensive care unit

    doi: 10.3892/etm.2018.5775

    Figure Lengend Snippet: Expression levels of TNF-α and MMPs in patients with MTB. Serum levels of (A) TNF-α, (B) MMP-1 and (C) MMP-9 were evaluated in patients with MTB. Protein expression levels of (D) TNF-α, (E) MMP-1 and (F) MMP-9 in human pleural mesothelial cells isolated from patients with MTB. Data are expressed as the mean ± standard deviation of three independent experiments. **P

    Article Snippet: The primary antibodies of rat anti-human anti-TNF-α (1:1,000 dilution, ab667), anti-MMP-1 (1:1,000 dilution, ab137332), anti-MMP-9 (1:1,000 dilution, ab73734), high mobility group box-1 protein (HMGB1, 1:1,000 dilution, ab18256), ERK (1:2,000 dilution, ab196883, Abcam), pERK (1:2,000 dilution, ab214362, Abcam), AKT (1:1,000 dilution, ab8805, Abcam), pAKT (1:1,000 dilution, ab133458, Abcam), and β-actin (1:1,000 dilution, ab8226; all Abcam, Cambridge, UK) were used to incubate with the membranes for 120 min at 37°C.

    Techniques: Expressing, Isolation, Standard Deviation

    Analysis of TNF-α-induced ERK/ATK signaling pathway in hPMCs isolated from patients with MTB. Expression levels of (A) ERK and (B) Akt were evaluated in hPMCs isolated from patients with MTB. Phosphorylation levels of (C) ERK and (D) Akt in hPMCs isolated from patients with MTB. (E) Expression levels and (F) phosphorylation levels of ERK and Akt were evaluated in si-TNF-α-treated hPMCs. Expression levels of (G) MMP-1 and (H) MMP-9 were evaluated in si-TNF-α-treated hPMCs. Data are expressed as the mean ± standard deviation of three independent experiments. **P

    Journal: Experimental and Therapeutic Medicine

    Article Title: Inflammation responses in patients with pulmonary tuberculosis in an intensive care unit

    doi: 10.3892/etm.2018.5775

    Figure Lengend Snippet: Analysis of TNF-α-induced ERK/ATK signaling pathway in hPMCs isolated from patients with MTB. Expression levels of (A) ERK and (B) Akt were evaluated in hPMCs isolated from patients with MTB. Phosphorylation levels of (C) ERK and (D) Akt in hPMCs isolated from patients with MTB. (E) Expression levels and (F) phosphorylation levels of ERK and Akt were evaluated in si-TNF-α-treated hPMCs. Expression levels of (G) MMP-1 and (H) MMP-9 were evaluated in si-TNF-α-treated hPMCs. Data are expressed as the mean ± standard deviation of three independent experiments. **P

    Article Snippet: The primary antibodies of rat anti-human anti-TNF-α (1:1,000 dilution, ab667), anti-MMP-1 (1:1,000 dilution, ab137332), anti-MMP-9 (1:1,000 dilution, ab73734), high mobility group box-1 protein (HMGB1, 1:1,000 dilution, ab18256), ERK (1:2,000 dilution, ab196883, Abcam), pERK (1:2,000 dilution, ab214362, Abcam), AKT (1:1,000 dilution, ab8805, Abcam), pAKT (1:1,000 dilution, ab133458, Abcam), and β-actin (1:1,000 dilution, ab8226; all Abcam, Cambridge, UK) were used to incubate with the membranes for 120 min at 37°C.

    Techniques: Isolation, Expressing, Standard Deviation

    Reverse transcription-polymerase chain reaction (RT-PCR) analysis shows the expression of mRNA for tumor necrosis factor α (TNF-α), IL-1β, superoxide dismutase-1 (SOD1), glutamate cysteine ligase catalytic subunit (GCLC) and matrix metalloproteinase-9 (MMP9). ( A ) Superoxide dismutase-1 (SOD1). ( B ) Glutamate cysteine ligase catalytic subunit (GCLC). ( C ) Tumor necrosis factor α (TNF-α). ( D ) Interleukin (IL)-1β. ( E ) Matrix metalloproteinase-9 (MMP9). * P

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Mercaptoethanol Protects the Aorta from Dissection by Inhibiting Oxidative Stress, Inflammation, and Extracellular Matrix Degeneration in a Mouse Model

    doi: 10.12659/MSM.905151

    Figure Lengend Snippet: Reverse transcription-polymerase chain reaction (RT-PCR) analysis shows the expression of mRNA for tumor necrosis factor α (TNF-α), IL-1β, superoxide dismutase-1 (SOD1), glutamate cysteine ligase catalytic subunit (GCLC) and matrix metalloproteinase-9 (MMP9). ( A ) Superoxide dismutase-1 (SOD1). ( B ) Glutamate cysteine ligase catalytic subunit (GCLC). ( C ) Tumor necrosis factor α (TNF-α). ( D ) Interleukin (IL)-1β. ( E ) Matrix metalloproteinase-9 (MMP9). * P

    Article Snippet: The aortic tissue sections were incubated with the following primary antibodies: rabbit anti-mouse nuclear factor (erythroid-derived 2)-like 2 (NFE2L2) polyclonal antibody (1: 1000) (Catalogue No. ab137550) (Abcam Biotech., Cambridge, MA, USA); rabbit anti-mouse nuclear factor κB (NF-κB) and p65 polyclonal antibody (1: 1000) (Catalogue No. ab16502) (Abcam Biotech., Cambridge, MA, USA); rabbit anti-mouse superoxide dismutase 1 (SOD1) polyclonal antibody (1: 1000) (Catalogue No. ab13498) (Abcam Biotech., Cambridge, MA, USA); rabbit anti-mouse glutamate cysteine ligase catalytic subunit (GCLC) polyclonal antibody (Catalogue No. ab53179, 1: 1000; Abcam Biotech.), rabbit anti-mouse tumor necrosis factor α (TNF-α) polyclonal antibody (1: 1000) (Catalogue No. ab6671) (Abcam Biotech., Cambridge, MA, USA); rabbit anti-mouse interleukin-1 β (IL-1β) polyclonal antibody (1: 1000) (Catalogue No. ab2105) (Abcam Biotech., Cambridge, MA, USA); and rabbit anti-mouse matrix metalloproteinase-9 (MMP9) polyclonal antibody (1: 1000) (Catalogue No. ab38898) (Abcam Biotech., Cambridge, MA, USA).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing

    Photomicrographs of representative immunohistochemical images showing the expression of matrix metalloproteinase-9 (MMP9). ( A ) Representative light microscopic images stained immunohistochemically for matrix metalloproteinase-9 (MMP9). ( B ) Statistical analysis for the representative immunohistochemical images. ** P

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Mercaptoethanol Protects the Aorta from Dissection by Inhibiting Oxidative Stress, Inflammation, and Extracellular Matrix Degeneration in a Mouse Model

    doi: 10.12659/MSM.905151

    Figure Lengend Snippet: Photomicrographs of representative immunohistochemical images showing the expression of matrix metalloproteinase-9 (MMP9). ( A ) Representative light microscopic images stained immunohistochemically for matrix metalloproteinase-9 (MMP9). ( B ) Statistical analysis for the representative immunohistochemical images. ** P

    Article Snippet: The aortic tissue sections were incubated with the following primary antibodies: rabbit anti-mouse nuclear factor (erythroid-derived 2)-like 2 (NFE2L2) polyclonal antibody (1: 1000) (Catalogue No. ab137550) (Abcam Biotech., Cambridge, MA, USA); rabbit anti-mouse nuclear factor κB (NF-κB) and p65 polyclonal antibody (1: 1000) (Catalogue No. ab16502) (Abcam Biotech., Cambridge, MA, USA); rabbit anti-mouse superoxide dismutase 1 (SOD1) polyclonal antibody (1: 1000) (Catalogue No. ab13498) (Abcam Biotech., Cambridge, MA, USA); rabbit anti-mouse glutamate cysteine ligase catalytic subunit (GCLC) polyclonal antibody (Catalogue No. ab53179, 1: 1000; Abcam Biotech.), rabbit anti-mouse tumor necrosis factor α (TNF-α) polyclonal antibody (1: 1000) (Catalogue No. ab6671) (Abcam Biotech., Cambridge, MA, USA); rabbit anti-mouse interleukin-1 β (IL-1β) polyclonal antibody (1: 1000) (Catalogue No. ab2105) (Abcam Biotech., Cambridge, MA, USA); and rabbit anti-mouse matrix metalloproteinase-9 (MMP9) polyclonal antibody (1: 1000) (Catalogue No. ab38898) (Abcam Biotech., Cambridge, MA, USA).

    Techniques: Immunohistochemistry, Expressing, Staining

    Expression levels of TNF-α and MMPs in patients with MTB. Serum levels of (A) TNF-α, (B) MMP-1 and (C) MMP-9 were evaluated in patients with MTB. Protein expression levels of (D) TNF-α, (E) MMP-1 and (F) MMP-9 in human pleural mesothelial cells isolated from patients with MTB. Data are expressed as the mean ± standard deviation of three independent experiments. **P

    Journal: Experimental and Therapeutic Medicine

    Article Title: Inflammation responses in patients with pulmonary tuberculosis in an intensive care unit

    doi: 10.3892/etm.2018.5775

    Figure Lengend Snippet: Expression levels of TNF-α and MMPs in patients with MTB. Serum levels of (A) TNF-α, (B) MMP-1 and (C) MMP-9 were evaluated in patients with MTB. Protein expression levels of (D) TNF-α, (E) MMP-1 and (F) MMP-9 in human pleural mesothelial cells isolated from patients with MTB. Data are expressed as the mean ± standard deviation of three independent experiments. **P

    Article Snippet: The primary antibodies of rat anti-human anti-TNF-α (1:1,000 dilution, ab667), anti-MMP-1 (1:1,000 dilution, ab137332), anti-MMP-9 (1:1,000 dilution, ab73734), high mobility group box-1 protein (HMGB1, 1:1,000 dilution, ab18256), ERK (1:2,000 dilution, ab196883, Abcam), pERK (1:2,000 dilution, ab214362, Abcam), AKT (1:1,000 dilution, ab8805, Abcam), pAKT (1:1,000 dilution, ab133458, Abcam), and β-actin (1:1,000 dilution, ab8226; all Abcam, Cambridge, UK) were used to incubate with the membranes for 120 min at 37°C.

    Techniques: Expressing, Isolation, Standard Deviation

    Analysis of TNF-α-induced ERK/ATK signaling pathway in hPMCs isolated from patients with MTB. Expression levels of (A) ERK and (B) Akt were evaluated in hPMCs isolated from patients with MTB. Phosphorylation levels of (C) ERK and (D) Akt in hPMCs isolated from patients with MTB. (E) Expression levels and (F) phosphorylation levels of ERK and Akt were evaluated in si-TNF-α-treated hPMCs. Expression levels of (G) MMP-1 and (H) MMP-9 were evaluated in si-TNF-α-treated hPMCs. Data are expressed as the mean ± standard deviation of three independent experiments. **P

    Journal: Experimental and Therapeutic Medicine

    Article Title: Inflammation responses in patients with pulmonary tuberculosis in an intensive care unit

    doi: 10.3892/etm.2018.5775

    Figure Lengend Snippet: Analysis of TNF-α-induced ERK/ATK signaling pathway in hPMCs isolated from patients with MTB. Expression levels of (A) ERK and (B) Akt were evaluated in hPMCs isolated from patients with MTB. Phosphorylation levels of (C) ERK and (D) Akt in hPMCs isolated from patients with MTB. (E) Expression levels and (F) phosphorylation levels of ERK and Akt were evaluated in si-TNF-α-treated hPMCs. Expression levels of (G) MMP-1 and (H) MMP-9 were evaluated in si-TNF-α-treated hPMCs. Data are expressed as the mean ± standard deviation of three independent experiments. **P

    Article Snippet: The primary antibodies of rat anti-human anti-TNF-α (1:1,000 dilution, ab667), anti-MMP-1 (1:1,000 dilution, ab137332), anti-MMP-9 (1:1,000 dilution, ab73734), high mobility group box-1 protein (HMGB1, 1:1,000 dilution, ab18256), ERK (1:2,000 dilution, ab196883, Abcam), pERK (1:2,000 dilution, ab214362, Abcam), AKT (1:1,000 dilution, ab8805, Abcam), pAKT (1:1,000 dilution, ab133458, Abcam), and β-actin (1:1,000 dilution, ab8226; all Abcam, Cambridge, UK) were used to incubate with the membranes for 120 min at 37°C.

    Techniques: Isolation, Expressing, Standard Deviation

    Expression levels of TNF-α and MMPs in patients with MTB. Serum levels of (A) TNF-α, (B) MMP-1 and (C) MMP-9 were evaluated in patients with MTB. Protein expression levels of (D) TNF-α, (E) MMP-1 and (F) MMP-9 in human pleural mesothelial cells isolated from patients with MTB. Data are expressed as the mean ± standard deviation of three independent experiments. **P

    Journal: Experimental and Therapeutic Medicine

    Article Title: Inflammation responses in patients with pulmonary tuberculosis in an intensive care unit

    doi: 10.3892/etm.2018.5775

    Figure Lengend Snippet: Expression levels of TNF-α and MMPs in patients with MTB. Serum levels of (A) TNF-α, (B) MMP-1 and (C) MMP-9 were evaluated in patients with MTB. Protein expression levels of (D) TNF-α, (E) MMP-1 and (F) MMP-9 in human pleural mesothelial cells isolated from patients with MTB. Data are expressed as the mean ± standard deviation of three independent experiments. **P

    Article Snippet: The primary antibodies of rat anti-human anti-TNF-α (1:1,000 dilution, ab667), anti-MMP-1 (1:1,000 dilution, ab137332), anti-MMP-9 (1:1,000 dilution, ab73734), high mobility group box-1 protein (HMGB1, 1:1,000 dilution, ab18256), ERK (1:2,000 dilution, ab196883, Abcam), pERK (1:2,000 dilution, ab214362, Abcam), AKT (1:1,000 dilution, ab8805, Abcam), pAKT (1:1,000 dilution, ab133458, Abcam), and β-actin (1:1,000 dilution, ab8226; all Abcam, Cambridge, UK) were used to incubate with the membranes for 120 min at 37°C.

    Techniques: Expressing, Isolation, Standard Deviation

    Analysis of TNF-α-induced ERK/ATK signaling pathway in hPMCs isolated from patients with MTB. Expression levels of (A) ERK and (B) Akt were evaluated in hPMCs isolated from patients with MTB. Phosphorylation levels of (C) ERK and (D) Akt in hPMCs isolated from patients with MTB. (E) Expression levels and (F) phosphorylation levels of ERK and Akt were evaluated in si-TNF-α-treated hPMCs. Expression levels of (G) MMP-1 and (H) MMP-9 were evaluated in si-TNF-α-treated hPMCs. Data are expressed as the mean ± standard deviation of three independent experiments. **P

    Journal: Experimental and Therapeutic Medicine

    Article Title: Inflammation responses in patients with pulmonary tuberculosis in an intensive care unit

    doi: 10.3892/etm.2018.5775

    Figure Lengend Snippet: Analysis of TNF-α-induced ERK/ATK signaling pathway in hPMCs isolated from patients with MTB. Expression levels of (A) ERK and (B) Akt were evaluated in hPMCs isolated from patients with MTB. Phosphorylation levels of (C) ERK and (D) Akt in hPMCs isolated from patients with MTB. (E) Expression levels and (F) phosphorylation levels of ERK and Akt were evaluated in si-TNF-α-treated hPMCs. Expression levels of (G) MMP-1 and (H) MMP-9 were evaluated in si-TNF-α-treated hPMCs. Data are expressed as the mean ± standard deviation of three independent experiments. **P

    Article Snippet: The primary antibodies of rat anti-human anti-TNF-α (1:1,000 dilution, ab667), anti-MMP-1 (1:1,000 dilution, ab137332), anti-MMP-9 (1:1,000 dilution, ab73734), high mobility group box-1 protein (HMGB1, 1:1,000 dilution, ab18256), ERK (1:2,000 dilution, ab196883, Abcam), pERK (1:2,000 dilution, ab214362, Abcam), AKT (1:1,000 dilution, ab8805, Abcam), pAKT (1:1,000 dilution, ab133458, Abcam), and β-actin (1:1,000 dilution, ab8226; all Abcam, Cambridge, UK) were used to incubate with the membranes for 120 min at 37°C.

    Techniques: Isolation, Expressing, Standard Deviation